LOCUS       DAZKPO010000001        93137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000001 DAZKPO010000000
VERSION     DAZKPO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.001"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACG8NZ_000001"
     CDS             <1..420
                     /locus_tag="ACG8NZ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0537712.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="ACG8NZ_000002"
     CDS             complement(504..1226)
                     /locus_tag="ACG8NZ_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0537713.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="ACG8NZ_000003"
     CDS             complement(1216..1953)
                     /locus_tag="ACG8NZ_000003"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0537714.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="ACG8NZ_000004"
     CDS             complement(1965..2957)
                     /locus_tag="ACG8NZ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0537715.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="ACG8NZ_000005"
     CDS             complement(2972..3715)
                     /locus_tag="ACG8NZ_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0537716.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="ACG8NZ_000006"
     CDS             complement(3956..5278)
                     /locus_tag="ACG8NZ_000006"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0537717.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="ACG8NZ_000007"
     CDS             complement(5265..6230)
                     /locus_tag="ACG8NZ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0537718.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="ACG8NZ_000008"
     CDS             complement(6227..7765)
                     /locus_tag="ACG8NZ_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0537719.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="ACG8NZ_000009"
     CDS             complement(8181..8468)
                     /locus_tag="ACG8NZ_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537720.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="ACG8NZ_000010"
     CDS             8602..9270
                     /locus_tag="ACG8NZ_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0537721.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="ACG8NZ_000011"
     CDS             complement(9561..10301)
                     /locus_tag="ACG8NZ_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537722.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="ACG8NZ_000012"
     CDS             complement(10431..11378)
                     /locus_tag="ACG8NZ_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0537723.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="ACG8NZ_000013"
     CDS             complement(11507..12271)
                     /locus_tag="ACG8NZ_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0537724.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="ACG8NZ_000014"
     CDS             complement(12275..12664)
                     /locus_tag="ACG8NZ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537725.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="ACG8NZ_000015"
     CDS             12799..13341
                     /locus_tag="ACG8NZ_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0537726.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="ACG8NZ_000016"
     CDS             13489..14121
                     /locus_tag="ACG8NZ_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537727.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="ACG8NZ_000017"
     CDS             complement(14255..14380)
                     /locus_tag="ACG8NZ_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0537728.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="ACG8NZ_000018"
     CDS             complement(14514..15407)
                     /locus_tag="ACG8NZ_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0537729.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="ACG8NZ_000019"
     CDS             complement(15589..16515)
                     /locus_tag="ACG8NZ_000019"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0537730.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG8NZ_000020"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG8NZ_000020"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0537731.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG8NZ_000021"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG8NZ_000021"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0537732.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG8NZ_000022"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG8NZ_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0537733.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG8NZ_000023"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG8NZ_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0537734.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="ACG8NZ_000024"
     CDS             complement(24786..25220)
                     /locus_tag="ACG8NZ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0537735.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="ACG8NZ_000025"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="ACG8NZ_000025"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0537736.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="ACG8NZ_000026"
     CDS             complement(26117..26992)
                     /locus_tag="ACG8NZ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0537737.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG8NZ_000027"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG8NZ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0537738.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG8NZ_000028"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG8NZ_000028"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0537739.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG8NZ_000029"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG8NZ_000029"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0537740.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG8NZ_000030"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG8NZ_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0537741.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG8NZ_000031"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG8NZ_000031"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0537742.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG8NZ_000032"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG8NZ_000032"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0537743.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACG8NZ_000033"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACG8NZ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0537744.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="ACG8NZ_000034"
     CDS             complement(35443..36210)
                     /locus_tag="ACG8NZ_000034"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0537745.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACG8NZ_000035"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACG8NZ_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0537746.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="ACG8NZ_000036"
     CDS             37323..37721
                     /locus_tag="ACG8NZ_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0537747.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37958..38500
                     /locus_tag="ACG8NZ_000037"
     CDS             37958..38500
                     /locus_tag="ACG8NZ_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0537748.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACG8NZ_000038"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACG8NZ_000038"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0537749.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="ACG8NZ_000039"
     CDS             complement(39286..39513)
                     /locus_tag="ACG8NZ_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0537750.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACG8NZ_000040"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACG8NZ_000040"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0537751.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="ACG8NZ_000041"
     CDS             complement(40048..40761)
                     /locus_tag="ACG8NZ_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0537752.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="ACG8NZ_000042"
     CDS             complement(40764..41660)
                     /locus_tag="ACG8NZ_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0537753.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="ACG8NZ_000043"
     CDS             complement(41818..42660)
                     /locus_tag="ACG8NZ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0537754.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="ACG8NZ_000044"
     CDS             42792..43445
                     /locus_tag="ACG8NZ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0537755.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="ACG8NZ_000045"
     CDS             complement(43508..44035)
                     /locus_tag="ACG8NZ_000045"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0537756.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="ACG8NZ_000046"
     CDS             complement(44055..45002)
                     /locus_tag="ACG8NZ_000046"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0537757.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46897)
                     /locus_tag="ACG8NZ_000047"
     CDS             complement(45014..46897)
                     /locus_tag="ACG8NZ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0537758.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(47060..49138)
                     /locus_tag="ACG8NZ_000048"
     CDS             complement(47060..49138)
                     /locus_tag="ACG8NZ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0537759.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="ACG8NZ_000049"
     CDS             complement(49151..49426)
                     /locus_tag="ACG8NZ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537760.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="ACG8NZ_000050"
     CDS             complement(49788..50243)
                     /locus_tag="ACG8NZ_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537761.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="ACG8NZ_000051"
     CDS             complement(50397..51605)
                     /locus_tag="ACG8NZ_000051"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0537762.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="ACG8NZ_000052"
     CDS             complement(51717..52049)
                     /locus_tag="ACG8NZ_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0537763.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="ACG8NZ_000053"
     CDS             52197..53072
                     /locus_tag="ACG8NZ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0537764.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="ACG8NZ_000054"
     CDS             complement(53154..53735)
                     /locus_tag="ACG8NZ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0537765.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="ACG8NZ_000055"
     CDS             complement(53741..55000)
                     /locus_tag="ACG8NZ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0537766.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="ACG8NZ_000056"
     CDS             complement(55002..56048)
                     /locus_tag="ACG8NZ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537767.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="ACG8NZ_000057"
     CDS             complement(56283..56558)
                     /locus_tag="ACG8NZ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0537768.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /gene="der"
                     /locus_tag="ACG8NZ_000058"
     CDS             complement(56801..58111)
                     /gene="der"
                     /locus_tag="ACG8NZ_000058"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0537769.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="ACG8NZ_000059"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="ACG8NZ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0537770.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /gene="cmk"
                     /locus_tag="ACG8NZ_000060"
     CDS             complement(59657..60337)
                     /gene="cmk"
                     /locus_tag="ACG8NZ_000060"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0537771.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="ACG8NZ_000061"
     CDS             complement(60386..61021)
                     /locus_tag="ACG8NZ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0537772.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="ACG8NZ_000062"
     CDS             complement(61087..62490)
                     /locus_tag="ACG8NZ_000062"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0537773.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="ACG8NZ_000063"
     CDS             complement(62477..63508)
                     /locus_tag="ACG8NZ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0537774.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="ACG8NZ_000064"
     CDS             63539..63760
                     /locus_tag="ACG8NZ_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0537775.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="ACG8NZ_000065"
     CDS             complement(63925..64662)
                     /locus_tag="ACG8NZ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0537776.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="ACG8NZ_000066"
     CDS             complement(64815..66155)
                     /locus_tag="ACG8NZ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0537777.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="ACG8NZ_000067"
     CDS             complement(66392..67915)
                     /locus_tag="ACG8NZ_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0537778.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="ACG8NZ_000068"
     CDS             complement(68293..68904)
                     /locus_tag="ACG8NZ_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0537779.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="ACG8NZ_000069"
     CDS             complement(69234..69950)
                     /locus_tag="ACG8NZ_000069"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0537780.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="ACG8NZ_000070"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="ACG8NZ_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0537781.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71279)
                     /locus_tag="ACG8NZ_000071"
     CDS             complement(70509..71279)
                     /locus_tag="ACG8NZ_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0537782.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="ACG8NZ_000072"
     CDS             complement(71276..71647)
                     /locus_tag="ACG8NZ_000072"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0537783.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="ACG8NZ_000073"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="ACG8NZ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0537784.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="ACG8NZ_000074"
     CDS             complement(72649..73122)
                     /locus_tag="ACG8NZ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0537785.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="ACG8NZ_000075"
     CDS             complement(73227..74090)
                     /locus_tag="ACG8NZ_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0537786.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="ACG8NZ_000076"
     CDS             complement(74286..76385)
                     /locus_tag="ACG8NZ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0537787.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="ACG8NZ_000077"
     CDS             complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="ACG8NZ_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0537788.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77738..79579)
                     /gene="dnaG"
                     /locus_tag="ACG8NZ_000078"
     CDS             complement(77738..79579)
                     /gene="dnaG"
                     /locus_tag="ACG8NZ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0537789.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79761..82076)
                     /locus_tag="ACG8NZ_000079"
     CDS             complement(79761..82076)
                     /locus_tag="ACG8NZ_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0537790.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82261..83121)
                     /locus_tag="ACG8NZ_000080"
     CDS             complement(82261..83121)
                     /locus_tag="ACG8NZ_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0537791.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83180..83812)
                     /locus_tag="ACG8NZ_000081"
                     /pseudo
     CDS             complement(83180..83812)
                     /locus_tag="ACG8NZ_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            83979..84740
                     /locus_tag="ACG8NZ_000082"
     CDS             83979..84740
                     /locus_tag="ACG8NZ_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0537792.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            84766..87357
                     /gene="mprF"
                     /locus_tag="ACG8NZ_000083"
     CDS             84766..87357
                     /gene="mprF"
                     /locus_tag="ACG8NZ_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0537793.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="ACG8NZ_000084"
     CDS             complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="ACG8NZ_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0537794.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            89100..89456
                     /locus_tag="ACG8NZ_000085"
     CDS             89100..89456
                     /locus_tag="ACG8NZ_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0537795.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(89697..90701)
                     /locus_tag="ACG8NZ_000086"
     CDS             complement(89697..90701)
                     /locus_tag="ACG8NZ_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0537796.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            90867..91718
                     /locus_tag="ACG8NZ_000087"
     CDS             90867..91718
                     /locus_tag="ACG8NZ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0537797.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            91735..91980
                     /locus_tag="ACG8NZ_000088"
     CDS             91735..91980
                     /locus_tag="ACG8NZ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537798.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            92167..92871
                     /locus_tag="ACG8NZ_000089"
     CDS             92167..92871
                     /locus_tag="ACG8NZ_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537799.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        25865 a        19521 c        15980 g        31771 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    76501 ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa
    76561 accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct
    76621 tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga
    76681 cgaatacgtt cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg
    76741 ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct
    76801 agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt
    76861 gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt
    76921 tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga
    76981 tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga
    77041 cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta
    77101 cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt
    77161 gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt
    77221 ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt
    77281 actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg
    77341 attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct
    77401 ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct
    77461 gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca
    77521 ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca
    77581 tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt
    77641 cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct
    77701 gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt
    77761 tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc
    77821 tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga
    77881 tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt
    77941 cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa
    78001 ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg
    78061 catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa
    78121 atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc
    78181 gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt
    78241 gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa
    78301 actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa
    78361 gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc
    78421 cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt
    78481 cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa
    78541 cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg
    78601 ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt
    78661 ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga
    78721 taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt
    78781 ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca
    78841 gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg
    78901 caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca
    78961 ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc
    79021 gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct
    79081 cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt
    79141 gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt
    79201 ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga tttcggacag
    79261 ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta
    79321 ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc
    79381 ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg
    79441 tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga
    79501 catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt
    79561 gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca
    79621 gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt
    79681 aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca
    79741 catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca
    79801 gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct
    79861 acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca
    79921 tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa
    79981 gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt
    80041 tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact
    80101 acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt
    80161 tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca
    80221 atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc
    80281 gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg
    80341 ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac
    80401 aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa
    80461 tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg
    80521 ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa
    80581 accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg
    80641 acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga
    80701 ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct
    80761 tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct
    80821 ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca
    80881 ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc
    80941 gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa
    81001 gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata
    81061 gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg
    81121 atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca
    81181 gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa
    81241 tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca
    81301 atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca
    81361 ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc
    81421 cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt
    81481 tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct
    81541 gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc
    81601 aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt
    81661 gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt
    81721 tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg
    81781 atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt
    81841 atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg
    81901 gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac
    81961 gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca
    82021 ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc
    82081 tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc
    82141 aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa
    82201 aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct
    82261 ttacttatat tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa
    82321 aagaacagga caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat
    82381 cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt
    82441 tgagaaaatg taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc
    82501 gatcggcaca atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa
    82561 tcccatatac accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat
    82621 caatccgccg gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg
    82681 agcgccgtat aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc
    82741 agaagtatct cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat
    82801 acatccgaca aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa
    82861 aaaagaaagt agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac
    82921 attttctttg aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc
    82981 aaagacagct gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa
    83041 cattcccttc acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc
    83101 cattttttgc cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga
    83161 ttgaacattt cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat
    83221 tcattttgac aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat
    83281 tgtaaagcaa ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa
    83341 gtgggctcag cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat
    83401 gattttttac ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata
    83461 aatcgcatcc ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat
    83521 caatactgtc agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct
    83581 gcatgagatc tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct
    83641 cgtagttttc catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca
    83701 ctcgttttac cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca
    83761 gaatgatcat gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac
    83821 ctagttattt tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc
    83881 cttgatttct gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac
    83941 gaaaatgatc gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac
    84001 tttacacagt aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga
    84061 gcgaaattta ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg
    84121 acggcagcgg ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt
    84181 atctagtaga tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc
    84241 agttaatggc cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt
    84301 ttcttggatt tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa
    84361 aagttcatcg attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg
    84421 ccagagtcat cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa
    84481 gtcttcgcaa aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa
    84541 atgatttaaa aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca
    84601 agctaaaaca ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga
    84661 aagaacaagg acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc
    84721 aatttctttc tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc
    84781 atacaatggc tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt
    84841 gtcattattg tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga
    84901 gaaacgtttg ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc
    84961 tctgtgttac ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag
    85021 aatcctcgtt acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt
    85081 ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc
    85141 agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt
    85201 tacagtctgc tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa
    85261 tattggatat ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact
    85321 ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta
    85381 gtttcattgt tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg
    85441 gaaatccgga ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga
    85501 atcgtgtcta tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg
    85561 tccgctattg gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc
    85621 tactacatca ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact
    85681 tttgatcagc gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc
    85741 gtggttgtct tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa
    85801 gcttttacag agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc
    85861 caatttccta gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca
    85921 cgtgtgaaac gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt
    85981 ttattaagtg attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt
    86041 cttgcttcta aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg
    86101 atcgatggaa tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat
    86161 ctgccggatt tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa
    86221 aagatttggt tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt
    86281 gttcatttcc ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc
    86341 ctgaagatcc ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat
    86401 aagcggatgt ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc
    86461 ttcaataata aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa
    86521 gcaatcgaag cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa
    86581 acaagcgaag agattgttat gattttgcat gaattcggct acgatttcat taagatgggg
    86641 gaagaagctt atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact
    86701 cgtgctgtct tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca
    86761 ttttctactg agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga
    86821 aaggaaaaag ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt
    86881 gcagttgtca aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac
    86941 acgaacaata aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct
    87001 ggcagtatgg atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa
    87061 tggttcaatt tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta
    87121 caggaaagga ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga
    87181 ttaagagaat ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct
    87241 agagatagtt ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt
    87301 gagcagacac aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa
    87361 ctgcataccg atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag
    87421 ttcatgcttc tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa
    87481 tcctgtttgg accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg
    87541 tttcttatgc ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa
    87601 ttgagaatgt cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca
    87661 cgttgtcacc aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat
    87721 cagttgtgcg gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac
    87781 cttcttcttt tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac
    87841 ctggtttagc aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca
    87901 cgcctacgtt atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg
    87961 ttacaactgc tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac
    88021 gaacagcacc acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga
    88081 cgtcttccac tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt
    88141 catctactgt atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg
    88201 cttttaatgc ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca
    88261 ataactcgcg aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa
    88321 ctttgttcaa gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt
    88381 cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt
    88441 gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg
    88501 cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt
    88561 cacgttcttc tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt
    88621 tgcctaatac tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa
    88681 tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca
    88741 aacctcctaa ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct
    88801 ttttttgact aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc
    88861 ttattatacg tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa
    88921 aaaagtacct tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt
    88981 aactttcttt tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca
    89041 acaactttca atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca
    89101 tgaaaaaatt aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag
    89161 cttatcatta tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa
    89221 tgtcagctac ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg
    89281 cttataataa agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat
    89341 taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg
    89401 aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa
    89461 tggtcttaaa attttattag ataaaaagag gctgggacag aagcgtttaa ctccaagaaa
    89521 taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg
    89581 ttctttactt ttgtctcacg ctcttttttt gttatattcg tcttttccga tccttctctt
    89641 gacacaattc atttaactga tcactggtac ttgaacacct aatattctag tcatttttat
    89701 aaacgattca atacttcatc cacatttttg atcatcacgg atatcgtacc atccggatta
    89761 ttcacaaatt caatcaaatc tgggttgcga tatacatcta ctggtacgat cagttcgatc
    89821 ccgtttgata gcttgaattt ttgttttcca aattttttct cagaaatttc ttgtacttct
    89881 tttacaggag gtgcttctgg gacaaatcct tgctcttgta cttcttcttt aaaagccatt
    89941 tttgccgtga tgttttcctt gaagactttt tccgcaatca tctcatgatc caaccgacct
    90001 ttttcttcta tcgtatcata gacagcttct ttcaaatcag ccatgatatc aaattcttct
    90061 gtctcaaatt tttttccaag atgcttggct actttttgga tcacttttac attttcttct
    90121 aaagaaggtg caggaacact ttcgatcact cttccagaaa aataaagctc ttttttgcca
    90181 gaaaactcat attttttttc aattaattcg taggtccaat cagtcaaatt cacagcgaat
    90241 gcttcatctg ctttttgtgt tttcgcccct agaattgctc gattcaaaat caagcggttg
    90301 tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag cctcacgata attgactttc
    90361 aataaagata agtacatcac tgtatctaat tcatacaaga caacaaatac atctgcacta
    90421 ggtgcttctt cactttcttg gtagatatcg aaccaccgct gaacgattgc ttcacttgct
    90481 tccacaaaat tttcttgagc gattttaagt gattcaacga tcatgctttc ttctgctagt
    90541 tgtccagttt ttgtttgagc agaagagatt ttttggatct tcttttgaag aaaatcacga
    90601 atatattctt ttgtcagatc cagctctttt tgtgaaaaga ctggagaacc agtttctctg
    90661 tcaataatat gcagaattgc tttttttaaa tagatatcca ttttctcatt cccttctgtc
    90721 ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa tcattttctt ttaaacaaaa
    90781 atttcaagag tagaaacaaa atggattctt tttttcgaaa aatgcgctac acttatagtt
    90841 gaacgaataa aaaaggagct gaaagtatga attcattaac aagcacttat cgtctatcaa
    90901 atggttacga aatcccggta gtaggattcg gcacatggca aacacctgat ggggatgtcg
    90961 ctgtttcctc tgttaaagaa gcgctggctg caggttatcg ccatatcgat actgcccaag
    91021 gatacaagaa tgaagaaagc gtcggccaag cgattaagga aagtggaatt cctagagaag
    91081 agatattctt gacaacaaaa ttatggaatg caaaccacag ctatgaactt gtgatgtctt
    91141 cttttgaaga atcattgaag aagctccaaa cagattattt agatctgttt ttgatccact
    91201 ggccaaaccc tgttgctttt cgtgataatt gggaacaagc aaatgcagat acatggcgag
    91261 catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat cggtgtgagc aatttcttac
    91321 cccaccatct agatacacta gcaaaaacag caaaaattat gccgatggtc aatcaagttt
    91381 tccttgcacc aggcgaatta cagacaactg tagttgagta tgcaaaaaaa catgatatga
    91441 tcctcgaagc atatagtcca ttaggcactg gtaaaatctt cgatgtgcct gaaatgaaac
    91501 aaatcgctga agcacatgac aagacgattg cacaagttgc tttgcgttgg tcgctgcaac
    91561 acgaattcct tcctcttcct aagtcagtga caccaagcag aatcaaagaa aatactgaat
    91621 tatttgattt tgaattaaca gaagaagaaa tgaaacaaat cgatcagtta gatggtgtag
    91681 tcggcaaagc aaaagaccca gatactaccc aattttaagt cgaaaggtga ttttatggat
    91741 ccaatcgaac gcttgaattc tctctcggaa gaagttaccc agacttttca ttccgatttt
    91801 gtctttttga tcgatgcaga gaaaatccaa cattttcctg caagaaactg gacacatgat
    91861 caaatcatcg aagaactgaa aaaacggttc gatcattcgc taatggtcaa accctggcac
    91921 gaacatgaag tcatttattc acctgaacta cctgtttttg ccttgatacc gaaaaaatag
    91981 aaagaatgga acggattatg tatatataca taatccgttc cattttttat ttttctttca
    92041 aaaaacggga aaatacgcaa atttttctgt tgtaatttgc gtatacgcaa gtttttttgc
    92101 attactcctt ttcatttggc tgtttttttc ttataatcga aagggtatga gcgaaggaga
    92161 gaacatatga agaaaaaaca gaaacaaaaa ttattaaatc aattccgtcc ttcattggat
    92221 ggtgtaagag gacaattatt ccgttctcta gaagaagaga gcttgcgacg ttatggtttg
    92281 ccgttacaag tcatgcttga tcccaaaaac cgtcaaatct taacgatcgg attagctggg
    92341 aaaacaaatg aagatacacg gatcaacgtg ccaatcgatg acaacttcaa tactgtatta
    92401 aagagaattc gttcaggaga aaacgggatt ttcgaacgat tccgtgacaa tcttcttatt
    92461 gaaatcgtgt cttactggaa tgatcaacga ttgaaaaacg atgaaccaac tgcgcaagct
    92521 gttgcgacac cagttacaga agaaacagtc agtgtgccag aagaaaaaac ggaaaaacca
    92581 aaagcggaca caactgaaaa tacacgctca actgatacga atgccgcttt aagttttgct
    92641 gatttttcaa aagccgttgc tgagtatcct aaattttaca cagaacaaaa tgaacatgaa
    92701 gtagttatta atgaaaaaaa tgctgatgaa cctcgtaaat tagcaactat ttctatgact
    92761 actgaaaatg agttcgttat cgaaaaagca ctcgaacgga aatacaaagt caaattgact
    92821 ttgattcctt tgatcgaaca atttgcagcg acagcgatta ctgatcgata aaaattctat
    92881 ttgtcataaa aggctgggat ttaaaatccc agccttttta gtcatataat aatttctaca
    92941 gcaagtaaaa agaaattcct tctaacatca ggctcaaata tcagcttgac ctgtccaaca
    93001 aatcacctat gatcctacaa tgttaggcga cttctcttct acctgaagat atcttggagt
    93061 tgcctgattt cttaagatgc ctaaccttaa ctataaaaaa ttctaataat aaacatattt
    93121 tctgatagag aaaaaag
//
LOCUS       DAZKPO010000002        87772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000002 DAZKPO010000000
VERSION     DAZKPO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.002"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..1107
                     /locus_tag="ACG8NZ_000090"
     CDS             106..1107
                     /locus_tag="ACG8NZ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0537800.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1126..2145
                     /locus_tag="ACG8NZ_000091"
     CDS             1126..2145
                     /locus_tag="ACG8NZ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0537801.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2247..2963)
                     /locus_tag="ACG8NZ_000092"
     CDS             complement(2247..2963)
                     /locus_tag="ACG8NZ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0537802.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2932..3717)
                     /locus_tag="ACG8NZ_000093"
     CDS             complement(2932..3717)
                     /locus_tag="ACG8NZ_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0537803.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3708..4457)
                     /locus_tag="ACG8NZ_000094"
     CDS             complement(3708..4457)
                     /locus_tag="ACG8NZ_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0537804.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4755..5279
                     /locus_tag="ACG8NZ_000095"
     CDS             4755..5279
                     /locus_tag="ACG8NZ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0537805.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5295..6794
                     /locus_tag="ACG8NZ_000096"
     CDS             5295..6794
                     /locus_tag="ACG8NZ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0537806.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6882..7484)
                     /locus_tag="ACG8NZ_000097"
     CDS             complement(6882..7484)
                     /locus_tag="ACG8NZ_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0537807.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7588..8544
                     /locus_tag="ACG8NZ_000098"
     CDS             7588..8544
                     /locus_tag="ACG8NZ_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0537808.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8849..9805)
                     /locus_tag="ACG8NZ_000099"
     CDS             complement(8849..9805)
                     /locus_tag="ACG8NZ_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0537809.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9979..10539
                     /locus_tag="ACG8NZ_000100"
     CDS             9979..10539
                     /locus_tag="ACG8NZ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537810.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10532..11356
                     /locus_tag="ACG8NZ_000101"
     CDS             10532..11356
                     /locus_tag="ACG8NZ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0537811.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11366..11983
                     /locus_tag="ACG8NZ_000102"
     CDS             11366..11983
                     /locus_tag="ACG8NZ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0537812.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12001..12684
                     /locus_tag="ACG8NZ_000103"
     CDS             12001..12684
                     /locus_tag="ACG8NZ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0537813.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13367..14650
                     /locus_tag="ACG8NZ_000104"
     CDS             13367..14650
                     /locus_tag="ACG8NZ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0537814.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14666..15553
                     /locus_tag="ACG8NZ_000105"
     CDS             14666..15553
                     /locus_tag="ACG8NZ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0537815.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15570..16406
                     /locus_tag="ACG8NZ_000106"
     CDS             15570..16406
                     /locus_tag="ACG8NZ_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0537816.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16409..17032
                     /locus_tag="ACG8NZ_000107"
     CDS             16409..17032
                     /locus_tag="ACG8NZ_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537817.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17029..18321
                     /locus_tag="ACG8NZ_000108"
     CDS             17029..18321
                     /locus_tag="ACG8NZ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0537818.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18680..18777
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18893..19474
                     /locus_tag="ACG8NZ_000109"
     CDS             18893..19474
                     /locus_tag="ACG8NZ_000109"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0537819.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19477..20784
                     /locus_tag="ACG8NZ_000110"
     CDS             19477..20784
                     /locus_tag="ACG8NZ_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0537820.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21200..21688
                     /gene="purE"
                     /locus_tag="ACG8NZ_000111"
     CDS             21200..21688
                     /gene="purE"
                     /locus_tag="ACG8NZ_000111"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0537821.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21681..22805
                     /gene="purK"
                     /locus_tag="ACG8NZ_000112"
     CDS             21681..22805
                     /gene="purK"
                     /locus_tag="ACG8NZ_000112"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0537822.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22818..24113
                     /gene="purB"
                     /locus_tag="ACG8NZ_000113"
     CDS             22818..24113
                     /gene="purB"
                     /locus_tag="ACG8NZ_000113"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0537823.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24235..25623)
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_000114"
     CDS             complement(24235..25623)
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_000114"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0537824.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26192..26569)
                     /locus_tag="ACG8NZ_000115"
     CDS             complement(26192..26569)
                     /locus_tag="ACG8NZ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537825.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26825..27553
                     /gene="purC"
                     /locus_tag="ACG8NZ_000116"
     CDS             26825..27553
                     /gene="purC"
                     /locus_tag="ACG8NZ_000116"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0537826.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27553..27807
                     /gene="purS"
                     /locus_tag="ACG8NZ_000117"
     CDS             27553..27807
                     /gene="purS"
                     /locus_tag="ACG8NZ_000117"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0537827.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27809..28480
                     /gene="purQ"
                     /locus_tag="ACG8NZ_000118"
     CDS             27809..28480
                     /gene="purQ"
                     /locus_tag="ACG8NZ_000118"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0537828.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28481..30703
                     /gene="purL"
                     /locus_tag="ACG8NZ_000119"
     CDS             28481..30703
                     /gene="purL"
                     /locus_tag="ACG8NZ_000119"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0537829.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30688..32127
                     /gene="purF"
                     /locus_tag="ACG8NZ_000120"
     CDS             30688..32127
                     /gene="purF"
                     /locus_tag="ACG8NZ_000120"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0537830.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32150..33202
                     /gene="purM"
                     /locus_tag="ACG8NZ_000121"
     CDS             32150..33202
                     /gene="purM"
                     /locus_tag="ACG8NZ_000121"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0537831.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33199..33777
                     /gene="purN"
                     /locus_tag="ACG8NZ_000122"
     CDS             33199..33777
                     /gene="purN"
                     /locus_tag="ACG8NZ_000122"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0537832.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33774..35309
                     /gene="purH"
                     /locus_tag="ACG8NZ_000123"
     CDS             33774..35309
                     /gene="purH"
                     /locus_tag="ACG8NZ_000123"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0537833.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35325..36599
                     /gene="purD"
                     /locus_tag="ACG8NZ_000124"
     CDS             35325..36599
                     /gene="purD"
                     /locus_tag="ACG8NZ_000124"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0537834.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36721..37635
                     /locus_tag="ACG8NZ_000125"
     CDS             36721..37635
                     /locus_tag="ACG8NZ_000125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0537835.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37768..39333)
                     /locus_tag="ACG8NZ_000126"
     CDS             complement(37768..39333)
                     /locus_tag="ACG8NZ_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0537836.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39326..40777)
                     /locus_tag="ACG8NZ_000127"
     CDS             complement(39326..40777)
                     /locus_tag="ACG8NZ_000127"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0537837.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40791..41807)
                     /locus_tag="ACG8NZ_000128"
     CDS             complement(40791..41807)
                     /locus_tag="ACG8NZ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0537838.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42027..43115
                     /gene="chvE"
                     /locus_tag="ACG8NZ_000129"
     CDS             42027..43115
                     /gene="chvE"
                     /locus_tag="ACG8NZ_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0537839.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43169..44695
                     /gene="mmsA"
                     /locus_tag="ACG8NZ_000130"
     CDS             43169..44695
                     /gene="mmsA"
                     /locus_tag="ACG8NZ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0537840.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44697..45902
                     /gene="mmsB"
                     /locus_tag="ACG8NZ_000131"
     CDS             44697..45902
                     /gene="mmsB"
                     /locus_tag="ACG8NZ_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0537841.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46159..47136
                     /gene="guaC"
                     /locus_tag="ACG8NZ_000132"
     CDS             46159..47136
                     /gene="guaC"
                     /locus_tag="ACG8NZ_000132"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0537842.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47400..48824
                     /locus_tag="ACG8NZ_000133"
     CDS             47400..48824
                     /locus_tag="ACG8NZ_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0537843.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(48866..49123)
                     /locus_tag="ACG8NZ_000134"
     CDS             complement(48866..49123)
                     /locus_tag="ACG8NZ_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537844.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            49285..50394
                     /gene="pdhA"
                     /locus_tag="ACG8NZ_000135"
     CDS             49285..50394
                     /gene="pdhA"
                     /locus_tag="ACG8NZ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0537845.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50397..51374
                     /locus_tag="ACG8NZ_000136"
     CDS             50397..51374
                     /locus_tag="ACG8NZ_000136"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0537846.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51399..53042
                     /locus_tag="ACG8NZ_000137"
     CDS             51399..53042
                     /locus_tag="ACG8NZ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0537847.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            53050..54456
                     /gene="lpdA"
                     /locus_tag="ACG8NZ_000138"
     CDS             53050..54456
                     /gene="lpdA"
                     /locus_tag="ACG8NZ_000138"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0537848.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            54618..54893
                     /locus_tag="ACG8NZ_000139"
     CDS             54618..54893
                     /locus_tag="ACG8NZ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0537849.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            54899..55666
                     /locus_tag="ACG8NZ_000140"
     CDS             54899..55666
                     /locus_tag="ACG8NZ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0537850.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            55834..57663
                     /gene="typA"
                     /locus_tag="ACG8NZ_000141"
     CDS             55834..57663
                     /gene="typA"
                     /locus_tag="ACG8NZ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0537851.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            57924..59696
                     /gene="recQ"
                     /locus_tag="ACG8NZ_000142"
     CDS             57924..59696
                     /gene="recQ"
                     /locus_tag="ACG8NZ_000142"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0537852.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            59810..60112
                     /locus_tag="ACG8NZ_000143"
     CDS             59810..60112
                     /locus_tag="ACG8NZ_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0537853.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            60115..61278
                     /locus_tag="ACG8NZ_000144"
     CDS             60115..61278
                     /locus_tag="ACG8NZ_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0537854.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            61304..64732
                     /locus_tag="ACG8NZ_000145"
     CDS             61304..64732
                     /locus_tag="ACG8NZ_000145"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0537855.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            64753..65877
                     /locus_tag="ACG8NZ_000146"
     CDS             64753..65877
                     /locus_tag="ACG8NZ_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0537856.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            65858..66304
                     /locus_tag="ACG8NZ_000147"
     CDS             65858..66304
                     /locus_tag="ACG8NZ_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0537857.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            66288..66626
                     /locus_tag="ACG8NZ_000148"
     CDS             66288..66626
                     /locus_tag="ACG8NZ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0537858.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            66745..67305
                     /gene="rsmD"
                     /locus_tag="ACG8NZ_000149"
     CDS             66745..67305
                     /gene="rsmD"
                     /locus_tag="ACG8NZ_000149"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0537859.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            67302..67793
                     /gene="coaD"
                     /locus_tag="ACG8NZ_000150"
     CDS             67302..67793
                     /gene="coaD"
                     /locus_tag="ACG8NZ_000150"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0537860.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            67783..68826
                     /locus_tag="ACG8NZ_000151"
     CDS             67783..68826
                     /locus_tag="ACG8NZ_000151"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0537861.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            68985..69713
                     /locus_tag="ACG8NZ_000152"
     CDS             68985..69713
                     /locus_tag="ACG8NZ_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0537862.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            69829..70074
                     /locus_tag="ACG8NZ_000153"
     CDS             69829..70074
                     /locus_tag="ACG8NZ_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0537863.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            70071..70289
                     /locus_tag="ACG8NZ_000154"
     CDS             70071..70289
                     /locus_tag="ACG8NZ_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0537864.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            70425..70964
                     /locus_tag="ACG8NZ_000155"
     CDS             70425..70964
                     /locus_tag="ACG8NZ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0537865.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            70957..73230
                     /locus_tag="ACG8NZ_000156"
     CDS             70957..73230
                     /locus_tag="ACG8NZ_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0537866.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            73268..74314
                     /gene="holA"
                     /locus_tag="ACG8NZ_000157"
     CDS             73268..74314
                     /gene="holA"
                     /locus_tag="ACG8NZ_000157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0537867.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(74403..74657)
                     /gene="rpsT"
                     /locus_tag="ACG8NZ_000158"
     CDS             complement(74403..74657)
                     /gene="rpsT"
                     /locus_tag="ACG8NZ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0537868.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(75057..75326)
                     /locus_tag="ACG8NZ_000159"
     CDS             complement(75057..75326)
                     /locus_tag="ACG8NZ_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537869.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(75588..75716)
                     /locus_tag="ACG8NZ_000160"
     CDS             complement(75588..75716)
                     /locus_tag="ACG8NZ_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537870.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(75796..76251)
                     /locus_tag="ACG8NZ_000161"
     CDS             complement(75796..76251)
                     /locus_tag="ACG8NZ_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0537871.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(76389..78317)
                     /locus_tag="ACG8NZ_000162"
     CDS             complement(76389..78317)
                     /locus_tag="ACG8NZ_000162"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0537872.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(78514..79347)
                     /locus_tag="ACG8NZ_000163"
     CDS             complement(78514..79347)
                     /locus_tag="ACG8NZ_000163"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0537873.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(79539..80243)
                     /locus_tag="ACG8NZ_000164"
     CDS             complement(79539..80243)
                     /locus_tag="ACG8NZ_000164"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0537874.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(80266..80892)
                     /locus_tag="ACG8NZ_000165"
     CDS             complement(80266..80892)
                     /locus_tag="ACG8NZ_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0537875.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            81218..83593
                     /locus_tag="ACG8NZ_000166"
     CDS             81218..83593
                     /locus_tag="ACG8NZ_000166"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0537876.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(83617..84108)
                     /locus_tag="ACG8NZ_000167"
     CDS             complement(83617..84108)
                     /locus_tag="ACG8NZ_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0537877.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            84295..85215
                     /locus_tag="ACG8NZ_000168"
     CDS             84295..85215
                     /locus_tag="ACG8NZ_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0537878.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            85369..85713
                     /locus_tag="ACG8NZ_000169"
     CDS             85369..85713
                     /locus_tag="ACG8NZ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0537879.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(85903..86403)
                     /locus_tag="ACG8NZ_000170"
     CDS             complement(85903..86403)
                     /locus_tag="ACG8NZ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0537880.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            86931..87014
                     /locus_tag="ACG8NZ_000171"
     tRNA            86931..87014
                     /locus_tag="ACG8NZ_000171"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:86965..86967,aa:Leu,seq:cag)
     gene            complement(87338..>87772)
                     /locus_tag="ACG8NZ_000172"
     CDS             complement(87338..>87772)
                     /locus_tag="ACG8NZ_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0537881.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        28251 a        15332 c        18785 g        25404 t
ORIGIN      
        1 aaaagctttt tttattgtga ataaatgaac ataattagga ggagaaagat gaacttattt
       61 gacatttttc gatcgacttt gacagatatg tcgattgtca gtgcaatcac ttcaacagtt
      121 tttattatat tattagggtt tgtttgtaga aaaaagggga ttttttctga atctgttgga
      181 aaaattctat cgaaagtcgt cttaacagtg gctttgccag cactggcatt tcgagctttc
      241 atgcaagata tcaatacaga attactgatg caaggaatca atgtcttgat ttggggatta
      301 gttatttata ttattcttat tctagtttca aaaccttttt tcttaagcta taaaggggat
      361 aagcaaacaa cgttgcgtgt cctgacgatc tttggttcta cgaccttttt cggaacgcct
      421 atcgtcagtg cgatttatgg tcctgtcggc gtgatgtatt cctcaatttt caatattggc
      481 taccgaatct ttttgtattc ttatggatac ataaaaatga gtggcttgaa gatggaagct
      541 aaaaatatca aagcgatgtt cgtcaatcca atcattatcg ctacttttgc cggccttttc
      601 atttggctgt tccaagggta tctgccacaa gtgtcagtta caggtgcaga aggagagata
      661 agccaagtag cgtttttgcg aattgaccag actgcaccgt ggctatttca gtcgatggat
      721 tatttagctg gtttaagttc accattggcg tggctagcaa tcggcgcaac attaggtgaa
      781 gtaagcttca aaaaagctgc atctgaaaaa acttcatggt attacagtct agtcaaagtg
      841 gtatttgtac ctgcattgaa tatcgtgatt ctagcagttt tgacggcaac gaatattcta
      901 ccagtcaatt ttgaagcact ggcaacaatc gttatcatga tggctacacc agctgcaaca
      961 gtggcagcag cctatgctat cagttttgat agggaggcac ttttagcctc caatgcatcc
     1021 ttactgtcaa ctatttttgc tgtggtactg acacctgtat ggatcgttgt attacaaatg
     1081 attcatggca taggtttatt ttcataagaa atggaggaag aaagtatgag aatcgcatgt
     1141 tacggtgttc gtccgaacga gatagatttt tttgaaaaat tgaatattta ccattatgat
     1201 ctcagtcttt atgaagaact gttgacacat gacaatatcg tgtcagcaaa ggaccatgat
     1261 gctgtattgt tgaggggaaa ttgtatagca gatgagacca atcttgcaaa aatgcaggaa
     1321 tatgggattc gttatgtttt tacacggact gtgggagtca atcatatcga tctacaagcg
     1381 gcagctgatt ttggtatgac agtagcccgt gtaccatcgt attcgccaaa tgcgatagca
     1441 gagttatctt tgacttttgc catgatgctt ttgagaaata ctgcgtatac gacgatccga
     1501 acatctttca aagattttcg agtggatgag caaatgttta gtcgtgaaat cagaaattgc
     1561 acagtaggga tcataggaac aggcaggata gggttgacgg aagctgtttt gtttaaggga
     1621 ctaggagcca aagtgatcgg ctacgatatt catcagacag aagctgctaa aaaagtattg
     1681 acgttccact cgcttgaaga gttattagcc aaaagcgata ttgtcagtat tcatgtacct
     1741 catatcccag gtgaaaacga tcaaatgatc aatgaaggat ttttagctca aatgaaaaaa
     1801 ggtagcatct tgattaatac agcccgtgga gagcttcaag ataatgaagc tattttaaga
     1861 gctttgaaaa gcaatcattt agctggtttt gcaaccgatg tttttgcaaa tgaaacagag
     1921 gtttttttcc gatccttcaa gccatgggag acgattccag accctgctat ccagcaatta
     1981 gtagaaatgt accctagagt tcttgtaacc ccgcatgtag gttcaaatac agatgaggca
     2041 ttatcaaata tgatttcgac aagtttcgag aactttcgtg aaataataga aactggaaaa
     2101 acgaaaaatg aggtatctct tcctaaagcc cgtcaattaa gataagaaaa catggatatt
     2161 cgtcaataaa atgtttaaaa atagtccaag taaacagttg ttacttctgt atacttggac
     2221 tattttgtat gggataattc gatgactcaa cagtaatctg tgagcttttt ccgcccattc
     2281 tttgtcaatt cataacaggt tagcggccgg ccgatttcca aatatttgat tctttcgttc
     2341 aatatatctt gttctactaa aaaattcaaa tatttcttga cggatatacg tgacagtgag
     2401 cttttttgag ccagttcttt tgttgaaaaa atagtatctt cttttagaat aaatgcactt
     2461 atttttttta aagtcagttt tgacagacct ttaggcaaat cgatgaatga tggtatttcg
     2521 tgaatgggag tggtgtggtt gaatacttga tcgatcaatt tctgatcggc tttttcagtc
     2581 gtttggattg tctgagagaa agcaagatac ttttcgatcg ccagctgaaa acgttgcaaa
     2641 gtgaacggtt tgatcaaata atctaaggca ccgtagcgga tcacattctt tatggtttcg
     2701 atatcatctg cagcagtgat caaaatcact gggatatcat ttccttgctg tctaagccaa
     2761 gataaaaaat ggataccact ttcgccaggc atgtatacat caagcagcag cagatctgcg
     2821 tttttttgtc tgaaatattt ttttccttct tctacatttc tgaaactttc gattacgttc
     2881 atattaggga tgctttccac aaaagtacga ttcaattctg caaccattgg atcatcttcg
     2941 attatcagga catttgtcat cattttgatc ttcctttttt ggtaattcta gatagacgat
     3001 tgtaccgcca tttgtttgtt tggaaaattc taaaaatcca ccgtggcgtt tgacgattga
     3061 atggatgaga ttcaatccaa acccacgatt gattcccttc gtcgaaaaac ctagcgtaaa
     3121 tattttttct gagatttctt ctgatatccc gacaccgtca tcgcagacgg tcacgatgaa
     3181 tacttcagct tctgtgtcat agtttaaatg aatccggacc tgtccgtttc tttcatgttt
     3241 ttgtagcacg gcttcttttg cattatcaag taagttccct aacacttgca aaagtgaatg
     3301 gatggagtca tccatctcca ttttagggat aaaagaatcg ctttctaaca gcatttcaac
     3361 agattgttct ttggcttcgt ttattttgcc taataaaaaa ccggcaattg caggtacttt
     3421 gatcagactt gagacttggc caatctcttt tttataattt tgatttagct gctggataaa
     3481 atgggtgact tcttcatact tcttcaaatc aatcaaacca gaaatgacat gaagtttatt
     3541 catgaattca tgggtttgag ctctcatgga gtttatatat tgtttcgtcc ccccaagctc
     3601 ctccattaat ttcattactt ctgatcgatt tcgtaaagtt gcgactgctc ctaaaaaacg
     3661 gtcattgcta aaaagaggtg taacgctaga aatagtttct atcccattta aatacatgga
     3721 ttgagtccga acggtcgttt gatgctcaat cgtctcttta aacaaaacgt catatagatt
     3781 atcactgatt tttttcctac ttcgaaatcc aatagctgct ctgtttggta gaaaatgttt
     3841 tttgcaggtg tattgatcaa gaggatagtt ttatcagaag agatggctaa gattccttca
     3901 tctacttcat tttcgataat cgttttttca cttaactgtt ctgctatttc ttgtggttca
     3961 agtccaagca gtactttttt cattttttgg gcaagaaaaa cggcgcctat gatgcctacg
     4021 ctaagtccaa taatcaatga aaaaacaatc attttttgtc catctttgac ttgattgtca
     4081 atcgtgttgt ttgttaagcc aacacaaact gcaccaataa tctcttgatc ataataaata
     4141 gggacgaaat agcggaagcc gaaccctaac attccttctt tttcagagaa aaattcttgt
     4201 cctccaatca cttttttcac atcttcatga cttgagaaag gaagaccgat gtaacttggg
     4261 tctgcgtgag aatatcgaat gccttcaggg tccatgacaa cgataaaatc taaattcgat
     4321 gcaatcatga tttcatttgt tattccttga atcgcatgtg tcgggtcacc agtctgcagc
     4381 tttttttgga tgagtttatc attggcaacg attttagcaa tgctagagat tttatctgac
     4441 acactagatc ggttcatcga aagaataaaa ttcctgatca atatggcaga aacaatcaaa
     4501 gaaacgaatg tggagaaaca aacgacaagg atgatcgtgt tctggagctt taatggtttt
     4561 ttcttaaaaa aaggaaacat aggctacctc gaaagaattt attgtgaaaa ttttaacatg
     4621 aaaacaaaaa tttaacaaat tgtcaacgat aaacacaacc ttcctataaa aaacaccact
     4681 gagtcggtaa aagaagctat acagaatttt caaacattgg tatgatagac gtagaaatgg
     4741 aaggaggcgt gtatatgata gggggaatcg cacttgagac gatttattgg tatacattgc
     4801 tgatttgtgc aggattggca gtcctattga ttatttttgg agatgtattc gattttgatg
     4861 gcccgcttga tccgatgctt attatccctt ggttaacttt cacaagctta tttggttttc
     4921 taggtgagcg tttaacagat caatcatctt tatttttatt tattgctagt ggaggcatat
     4981 cgatagtgtt ggtatttttg cttaatttct atatattgat gccgatgaaa catgccgaat
     5041 caacgatgtc gtcttctgaa aaaactctgg aaggacaagt agctgttgtg atcacaccga
     5101 tccctgtacg aggtatggga gaaattcagt tgaaaagtgt taccggttcc attagtcgtc
     5161 cagcttgttt ttacagcccc caagaacaga cagctgagcg gggaacgaaa gtattgatca
     5221 ttgagatcaa ggaaagaatc tgttatgtca tgccttatga aagcatgata aaagcttaaa
     5281 ggagcgtgtg ttaaatggaa ataccagttg tttttatcgc gatcgcaatc gcagctttga
     5341 ttttactgat gttgctgatc gtctttgtag cgaagtacca gacagcaaaa ccggatgaag
     5401 cgttgatcat cagtggaagt tatttaggaa gcaaaaatgt gcacattgat gaaagcaata
     5461 acaaaatcaa aattgtgcgt ggaggtggcg cattcgtctt gccagttttt caacgttcga
     5521 atcggatcag tctattatcg agtaaattgg acgtctccac ccctgaagtg tatacggaac
     5581 aaggcgtacc ggtgatgtgt gatggtactt cgatcatcaa aatcggttca tctgtagaag
     5641 agattgctac agcagcagaa cagtttttag gcaagacaag agaagaactg gaaaatgaag
     5701 caagagaagt attagaagga cacttgcgct cgattcttgg ttctatgact gtagaagaaa
     5761 tctatcaaaa tcgcgataag ttcagtcaaa gcgtacaaga agtcgcttct gtagaccttg
     5821 caaaaatggg gttgatcatc gtttctttca ctatcaaaga ggtcagagac aaaaacggtt
     5881 atcttgactc tttagggaaa ccaagaatcg cccaagtcaa aagagatgca gatatagcag
     5941 aagcggaagc tctaaaagaa acgcgtatca aaaaagctca agcagaaaaa gaatcacaag
     6001 cagctgaatt acagcgacaa acagagatcg cagaatcatt aaaagaaaaa gaactgaaat
     6061 tagcaaccta caaacaagaa caagatgttg ctaaagcaaa agcagaccaa gcctacaatt
     6121 tagaaagtgc ccgagcacaa caacaagtaa tcgaacagga aatgcagatt aaaatcgtag
     6181 aacgtcaaaa acaaatcgaa ctggaagaaa aagaaatcac acgtcgtgaa aagcaatatg
     6241 attcggaagt gaagaaaaaa gcagatgccg atcggtacgc taaagaacaa gaagcacaag
     6301 ctcaaaaagt caaagaagtg actgaagccg aagcagaacg attccgtgtg gaagcactgg
     6361 cagaagcgga agccaataag acacgtttag ctggacaagc agaagccgaa gcagcactag
     6421 caaaaggaaa agcagaagcc gaagcaaaac aaaagatcgc caatgccttc aaggaatatg
     6481 gcgaagcagc tgtcttgagt atggttatcg atatgctacc gcaattgatg cgcgaagcag
     6541 cacagccgct aggaaatatc gaaaaaatct ctgttgtcga tacaggaagc agttcaggag
     6601 aaaccagtgg agcgaaccga gtgacaaatt atgccacaaa tttgctttct tctactcaag
     6661 aaacactaaa agcgacaaca ggacttgact tgaaggaaat cgtagagaat ttgtcgacac
     6721 gtgggactac tagccaagta aatatgtatg aagcgccaaa agaggcagaa caaaactcga
     6781 tgaaggaaga ataaagtagt acacttcacc gttcaatcaa aaatggagga agacttttta
     6841 aagttcttct ctccattttt ttattttatt gcaaaagaaa attagtcctc gctatttttc
     6901 ttttgttttt ggatcttagc ttctatctct tgtaaatttg ttcttagcaa tcgttctttc
     6961 ttttggagtt cgaccaaatc ttctgatagt tcttccttct tttctgtttc aggaggacga
     7021 agaaagaaat tgataccgat tgcaatgaat gtccctataa aagtatctag tacacgttgg
     7081 atagcataca aagcggattc gccttgaggg atgctcaacg caatcagcaa caacgtagct
     7141 attgcggaaa tgataccgga attattgtta ataccatctg acacgacgat cacaataata
     7201 actagaatag gaagcaagat caattctacc aaaaaatcat tgtgaaaata gcttttgaca
     7261 aaaaaataaa tgatagctaa tgtaccacct aatgtattgc cgatgatcct agactttcca
     7321 aaagaaacgg ttgtagtcaa gtcctgacgc aaagaaaaaa cagcagataa tgcagcaatc
     7381 atcgggattc ctcgttgagt caaatgaaaa aacaagatac ataaaaaaac agctaaggca
     7441 cttttgatcg tacgcatacc tagtcgaaaa cggccaaatt gcatagagat tctccttttt
     7501 ggtcttttta ctatcatacc taaacttttg tctgatagct agtttttcat tattcaagta
     7561 taattaaaat aaaaaagggg gaagtaaatg cgtataggaa tgcgaacagt caaaacagtg
     7621 atcagtgtct tcttttcgat ggtcacagcg ggtagcttat cgctgttgta ttggccagct
     7681 gcagggatca ttgcattatt aagtgtagga aataccaagc gctctacatt gcgtacgggc
     7741 atgaatcgaa tcgcagcctt tatattagct acggcaacag cactgttcag tttttttgct
     7801 gttgggtata ctatttttgg cttcagtctt tttttattcc tgttcatccc gcttgcctct
     7861 cgcttcaaat taaccgaagg gatagtagta aattcggttt tggtcactca ttatttagtg
     7921 gaggaaaaca tgtcttggca gctgatcggc aatgaagcgg gattaatggc gattggatta
     7981 gtatttgctt tgcttgcaaa tgtttacatg ccagatcgta cgaagcagct gaaggaaaac
     8041 caaatacaga tcgaaaaaga atttcgaaat attttgagac aaatggcaga atttctttta
     8101 tcagaaaata aagaagatgt gcagatcaaa tgcgaacatt tactgacatt cattcgcgaa
     8161 agccaagaag atgccagaga acatcaagaa aactactggc tcagacagcc gctatactac
     8221 gaaacttact tctctatgag aagagcacag gccaatgtga ttaaagacat gctggaaaat
     8281 ctagagagaa tccagcaacc agcttattat ggaaaacata tctatggttt attgatttat
     8341 actgcagaaa cattttcaga gtcaaatgat ggcagacaaa ttttaacacg tattgaagaa
     8401 gtttatgtat tgtatcggca aatgcctctg ccgactagtc gccctgaatt cgaggatcga
     8461 gcagaattgt tccagttttt gcagtctttt aaaagtttta ttgaaatcaa ggcagaattc
     8521 tcccaacaaa aaatccaaaa atagaggttg tgaattcagc taatattcag gtcaaaaagc
     8581 tgatttcaca acctcttctt taaacggtgt tctcctgtca actcctcggt cacaagtcac
     8641 aatattcaac aaacatgaga agctgttttt tgaaattgct ccttgtgact cggagctgaa
     8701 cggtaggctc acaacctctt tcttaaacgg tgttcaaaag ctttcctctg cggtaataag
     8761 tccagccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     8821 agaacagctt tttcacaacc ttttaaaatt acatattttt ccatagaact tgcatttgat
     8881 ccaagatttc gatgacatct tttgtcaaga agagagagcg attgggcaag tcaccttcaa
     8941 tcatgtgtac catattcttt atttcgtaat tcattgcttc agatgtattc ccgctttcga
     9001 tgaattcctt tgtaccatct gtaaataaga tctccgcacg atcagcacgc gggtaatcag
     9061 caatcgtcac ataaccgttc tcaaaggcaa cgataccgac ttttggcatt tttgcctgaa
     9121 aagtcaagct gacagcagca agttcatttt ctttgttacg caagatagtg acggattgtt
     9181 cgtccacgcc tgtttcaaaa ggaaccattg tagaggcaac tacttcaggt tgagagctca
     9241 aaaagaaacg agcgaaagat acagcatacg taccgatatc caacagagct cctccagcta
     9301 attctggatt gaagaaccgg tttttagggt ctggttcttt gtaactgccg aaaggtgctt
     9361 ggatcatttt taaagccccc agttttcctg tatccattat tgagcgtaac tgctgataaa
     9421 gtggcatgtt gaagattgtc atcgcttctg ctaagatcaa attcttttct tctgctaagc
     9481 gcatagcgtc agccagttct tttttattca tcgtgatggc tttttcacat agaacgtgtt
     9541 tgcctgcttc caatgctgcc aagatatggt cgatatgctg tctatttggt acagcaatgt
     9601 acacgatgtc gatttcagca tcgtctaaca tttcttgata gcttccgtaa gcttttggaa
     9661 tattgtaacg gtgagcgaag ttttccgctt tttctgaagt tctggaagca actgctgtta
     9721 gttcgctcgt ctcttgattg aagctttcgg caaactcatg tgcgatggta ccaagaccaa
     9781 cgattcccca ttgatatttt ttcatctgaa aacgctcctt catagttgga ttgagtttct
     9841 tgtctgtcta ttattataac aaaaatcacg aagaataaag aggagaggac ttgaaacatt
     9901 aaaaaaaatg aaagcagcga tttaagaggt gctttttgat caaaacttaa gtagaatagt
     9961 taaaaggagg ggataaacgt gcatcaagta tatattgatc ttattatgag tgtattatta
    10021 gaacagtttg aaactgaaaa aaatttttta gatgaatact tgctgatcag tccagaacaa
    10081 tggcaaaaat ggaaagatgg cgagaaaaat ctgccatctg aagcgatgca gaaagtaaaa
    10141 aatcttttca ctgattatga atggatgcta acgcaaaaga ttttgcgtca aacgattatt
    10201 tttcccgaaa aacgaaacgt tgccgttgca gagtacaagc aaatcaaaac acgcatcgcg
    10261 caaaaatggc ttagcagcaa agcagcaaaa gtcgagctga tccctttgaa aaataatcag
    10321 caggctggta gataccttga cttgaaagta agtattcaat atgatgaatg gggctatgat
    10381 gatattttga attttcgtct gccagcagat gttcaaaaac agatcgaagg agaaaaaatc
    10441 gaacttctcg attgggtgaa tgaaaatctg gaagaaactt acgttcatga ggcgaatggg
    10501 gaacgtgaag aacaacgaaa ggatttttca agtgaataaa tttttaggat ttggagcagt
    10561 tgtgattatg atcgcagcaa tgggctatgt tatttttagc gatgatgatt cgcatgttca
    10621 agccggcaat caagcagtcg aacgaacaag caccaaaaca actgctagta aagaaaagaa
    10681 cagtcaaaca accagcagca aggtaaagga caaagaaaca aaagatcgta cgtccaaaca
    10741 attgccagat gttaaaacag acgattggaa gctagttttg gttggtccag atcacaaatt
    10801 agaaaaagaa attgatgagt ctactcaatt ggcatcatta agtaacggat acttgatcga
    10861 caaacgcatt gctccagaat atgaagcatt tgccaaggct gctgaagcag ctggatttcc
    10921 attagtgatg gtctcagctt accggtctgt atcatcccaa caacaagttt tcgaacaaaa
    10981 tgtccaagat gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa agattactaa
    11041 agaaacgatt acggagccag gatacagcga acaccacaca ggtctggcag tcgatgtcgt
    11101 cgatcaaaac tggtacaaca gttatccttc tactgtattg gatgcaagtt acggagatca
    11161 gcctggagct aaatggatcg cagataatgc tgcgcagtat ggatttatcg ttcgttatcc
    11221 aaaaggacac gaagatataa ctaagatcac atatgaacct tggcacttac gttatgtcgg
    11281 taaagaaaac gctgagtata tcaccaaaca ccaattgacg ctagaagaat atctgaatga
    11341 attaaatgaa aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc aaaagcgcaa
    11401 gaatatccgt tggccagcag tggttgccgg tgtagcagtg atactcttag cctttgtttt
    11461 ttccttgaag aatctgtctt ctccttttac tgataatcag caagaaaatg aagcgcaatc
    11521 ccatcagcaa tttattgaac ggcttgttcc gcatgcccaa gaactacaag atggttatgg
    11581 agtgcttcct agtatcattt taggacaagc tatattagaa tcaaattggg gaaaaagtac
    11641 cttggctagc aaatacaata atcttttcgg gatcaaggct tatggggatc agaagaaagt
    11701 aagcctggaa acaaaggaat ttgttaacga agaatggata actatccaag gagacttcaa
    11761 agtgtatgat tcatgggaac agtccatgga tgaccataca caactgttcg tccaaggagt
    11821 agattggaat cctgcgctgt atgaaaaagt gattacggcc accaactatc aggaagccgc
    11881 acaagcattg caagatgcag gttatgcgac agaccccggt tatgcgcaaa aaatcatcca
    11941 ggtcatcgag acgtatcaat tgaatcagta tgaccggaga tagcaaagga gaagtggctg
    12001 atggcagaac aatttacgcc agaaatcatg acggaattga gaaaagatat cgtaagagta
    12061 ccggaaatca ttttagaagc aagcgggatc aagatatttg gaaaattgat ccgttcgatc
    12121 atctttacta cggatattgc gatcatacgt aatacaaatg cgaatgcagt aattgctgtg
    12181 tatcctttta cacctcatcc agcgattact aaaagcatta ttgaggcagc tgacattcca
    12241 gttttctccg gtgtaggtgg tggtttgaca caaggtgctc gttcttccta catgagcatg
    12301 ttcgcagaag cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc tttagagaca
    12361 gtaaaagaag tttgtcgagt agttgatatt ccagttatca gtacggtaac gtcgaaatat
    12421 acagaaatag atgaaaagtt agcagctggt atttcgatta tcaatatcag tgcaggtaaa
    12481 gaaactgcag aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc tatcattgcg
    12541 acaggtggtc caacggaaga aagtattgct gagaccattc aagcaggagc aaatgccatc
    12601 acatatacgc caccaagtag cggagagctt tttagtcgta aaatggaaaa atatcgggaa
    12661 actgagaaaa agaatcatga ataacaggtg agaatcaaaa gacgaggaag agcagatagt
    12721 ggctcttcct cgtcttttgt actccagtac tatttgttca gaattcaaaa aaaagagaaa
    12781 tttttttgtt tttttacctg taatgtacat aagaagagtt aaaacctttt gttattatta
    12841 gcattctaac aaaaaaatgt cttgatttat tgaagcgctt tctatagaat gaaaaacgaa
    12901 taaaaactaa aaaaggcacc cgacaattta gtagcagcta aattgtcggg gcttgaaaag
    12961 tcagcgggaa ctgaccaatc aagttgccat agtcaatgct tgtgcatcga cgtgtatatt
    13021 gtacctaatt tcatacagaa ttactagtta tttttctgtt ttttagggaa gagcgcacac
    13081 gaagaagcat gcttatttag ctatgtcaag gatattgggc agttgtctga tatccttttt
    13141 tctttgcgta attatcctcc catcattgaa gcaagaagtg tttttagcga tgaaagaaga
    13201 atcagcacta tctaattttt cttgaaagta ttgagttgtt catcctaggc agaacgataa
    13261 ggcaagtctt ggtttttatt ccccttgaaa aagatcgttt aataaatatg ttctgtataa
    13321 aaatacagta tattgaaaaa taacaatgga agtagaggtg cagagaatga agaaaagaat
    13381 gagaatcatc ttagctttaa gtgttatgtt attcttcttg acggcttgtg ggcaagaaga
    13441 tgacaaaaaa atcacgctga acatggcttg gtggggttcg caagtccgac acgatgcaac
    13501 agtaaaagta atcgagcttt atgaagaaaa aaatccccat gtgacgatcg attatgaatt
    13561 ttatgattat gaaggatatt acacaaaatt aggtacacta gcagcatcag atgatatctg
    13621 ggacattttc caactgaaca atcaatttcc tcaatacatc aatcagattg aaccattaga
    13681 tgaatatgtg gagcaaggga taattgatat ttctaatgca gatgaatcct atttagcaac
    13741 gacgacttac gacgggcaat tagttggact gtctaacggg gtgaattcgt ttgcgattgc
    13801 ttacaataaa aatatatttg aacaattaaa tatccctgaa ccagcttcaa attggacatg
    13861 ggatgaattt gaacaaatca gctatcaaat cactgaagaa ttaggaatct atgctatttc
    13921 aagattcgaa gattttatag ctggctgtat tattcaaata ccacaagttg agaaaggatt
    13981 gaatttttat aatcgggaag atctatctaa cagcttggga tttgaaaatc cagactatct
    14041 aacagatttt ttccagctgc gtaaaaatct ggtagacaaa ggggcccatc cagaaccagg
    14101 tggtggagct gcaagtacga cggatctgtc ttttcaggcg gttagggata gagaagcggc
    14161 aatggtgttt ctatcaagta accagttgac agaaatattg tcaggggctc ctgaagatat
    14221 ggagattcag ttgattaatc cacctcgcag aaaagcagat ggagaatctg gtatcccttt
    14281 acgttcttcg caaatgctga gtatggcaaa aagctcagcc cataaagaag aagctgctaa
    14341 gtttattgat ttctttcaaa acagtgaaga agccaatgaa attttaaaag gagaacgtgg
    14401 tgtaccaatc atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg caactttgca
    14461 agcgacattt gattatttag aaatgatttc cgatatggac aacggcgaag tgaatgtctt
    14521 tgatagtgct ttgaatccgg aaattcaaga ccaatacaat cttttagttg aaaaagtcat
    14581 ttatgaagaa gtaacagcag aagaagcagc agagagtctt tatgagtttg cgtcaaacat
    14641 tctaggatag cagcaggagg aaggcatgaa aaaagaaaaa ctaaaacggt tcttggatca
    14701 agacaatgtg gtcggttacg tatttgcttc tccattcatc ctaggatttc tattgataac
    14761 agtgataccg atgatcttgt cactttatta ttcgttcacc gattatcgtt tgggaaatcc
    14821 agtctcatgg atcggcttgg ataattatat aaagatgctg agtgatgccc gttttttgaa
    14881 ctccatccga gtcacttttc tctatgtatt tgcatctgta cctgtgaaac tgatctttgg
    14941 tctattgatt gcttatcttt taacgaggaa tatcaaagga acgactttct ttcgttcttt
    15001 gtattatgtg cctagtttga tcggtggcag tatttctatc gctctagttt ggcgggagtt
    15061 gttttctaaa aacgggttag tgaatatttt cctggaacga ttaggcgcac ctgcgctttc
    15121 ctggtttagc gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg cttggcaatt
    15181 cggttcggca atgatcatct ttgctgcagg tatcaaagag atcccaggct cgtattatga
    15241 agcggcagag atcgatggag cgaacaaatg gcagacgttt tacaagatca cgttacctct
    15301 tctttcgccg atcattttgt ataacttggt tatgcagaca atcgcggcgt ttatgacttt
    15361 tacgcaggca tttatcatta cccaaggtgg tccgaatgat gccacgaact tttatgctct
    15421 ttatgtgtat aaccaagctt tttcttttaa ttctatgggg tatgctagtg ctatttcgtg
    15481 gatcatgctg ttgattatgg gagctgtcac ctttctgatt ttcaaatcat caaaaaaatg
    15541 ggtcgttgtg tgaatgagag gaggaagaaa tgaataatac agtaacgaaa tggtttcaat
    15601 acggttttat tttgctatta ggtctagtca tgatttatcc ggtcttatgg atgatcgcag
    15661 gttcttttaa aacggatcaa gagattatga gtggttcgct gaatttgatt ccagagactt
    15721 ttcaatggga aaattacgca aaaggatggg cggggtttgc tggaatttca ttttttactt
    15781 tctttaggaa ttcgtttgtc atctctgcag tttcgacgat agggacagtt ctgagctcga
    15841 cttgtattgc ttacgcatta tcgagaatcg atttcaaagg aaaaagattc tggttcgtcg
    15901 tcatgttagt aacgatgatg attcctgcac aagtgatttt gattccgcag tttattattt
    15961 acaatcggct gaatttggtt gggacgtatg tgccgctgat tttaccacat ttttttggac
    16021 aagcattttt catttaccag atcatgcaat tcatggtcag catccccaaa gagttagatg
    16081 aatcagcgat tattgacgga tgcagcaagt atagtgtctt taccaaaatt atttttcctt
    16141 tgctgaaacc ttcgatcatc acgacgatca tcatccagtt ttattggaaa tgggacgact
    16201 tcatgggacc attgatttat ttaaacaagc cgagatctta cacggtatcg attgccatca
    16261 aattatttgc agacgctgga tctagtacga cttacgcgag tatgtttgcg atgtctacgt
    16321 tgtcgctgct accggttttt ctgattttct tattcttcaa taaatatctt gtccagggga
    16381 tcagtacaag cggtctgaag ggctgaatat gaaaaagcca atagaagaga tgtttcttta
    16441 caagctcaac tactatgtct gtcggatttt tttaaccagt cttatcagta atgctctttt
    16501 cgctttagtt ctaggatgtt tgttcacttt aggattagtg ctgtctgctc gtgttgtcta
    16561 tctgatttat tttttgatga tgtatccttc ttattatcta tctgtccatt acttggcaga
    16621 aaaaaaggta tgttggagga aaggaaaaat caaacggatg tgtttcatca ttacgttgtt
    16681 tcagctgttt gtcattattt ttgcagttga ttggtattta ttagtgggaa agattggagc
    16741 ttggtatctg attccttttc tattgtgtct gttgcttttt gtatcccata gtttttttag
    16801 tacgctaatt gatatctgca aagaagataa agcagatagt cttgtttatt tgatcaaaag
    16861 gaatatctgg ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat gccttttgta
    16921 tggactgttc tggtgtatac agcatgattt tttactcact gcattactgt atccagggct
    16981 gccatttgtc acagtatttt gcttaaaaga aaaggagaat gactttaaat gaagaaaaaa
    17041 tatgtacaag tcggacttgg tgggagagcg cgtttttttt atgaggcaat tgcctctacg
    17101 tatcatcaaa cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag gatggattat
    17161 gcaaatcgga tacttgtgaa caagtatgga cagaaaaaag taaagacatg tatgccatca
    17221 caatttgagc aactactgga tgacgtacgg ccggctgaag tcattattac gtcagtagat
    17281 cgaacgcatc atgattatat cattcgtgca atgaaaaaag gatatgatgt tatttgcgaa
    17341 aaacctctga ctatcgatga ggaaaaagct caagaaataa taacggtcca aaaacagacg
    17401 ggaagaagcc tgcgagtcac ttttaattat cgttatgcac cgtatcacac gaagataaag
    17461 gaactgatag aaagtgatgt gattggtgat gtgttttcta tccacttcga atggctgtta
    17521 aatacgcaac acggggcaga ttatttcaga agatggcatc gaaataagca caatagcggc
    17581 gggcttcttg ttcataaatc tacccatcat tttgatctag tgaatttttg gttgaacagc
    17641 cgtccccaaa aggtatttgc attcggtgga ttaaactttt atgggatgca taatgcggag
    17701 aaacgcggag tagaaatatt ttacagtcga tgctatggaa gcgaactagc taaagctgat
    17761 ccatttgcta tagacctgtc agccaatgag gaattgcgag aaatgtattt agaagcagaa
    17821 aaagacagtg gatatattcg tgaccgcagt gtattttccg atgatatctc tattgaagat
    17881 acactaggcg taacggtcaa atatgaaact ggtgcgattt tgacgtattc gctaaacgcg
    17941 tatatgccgt gggaaggatt caatgtttct ttcaatggca gcaaaggacg gatcgaaatg
    18001 aaagtgatcg aaaagtctta catcaatgca ggtggagata aaaaggatga aggagcagcc
    18061 gttcttcagt ccatccatgt ctatccgatg ttcggtgagc catatgaggt aatcgtcgaa
    18121 caagaagcgg gcggacatgg cggaggagat ccgcgtttat tagatgatct atttggtgaa
    18181 gaaaaagaag aagatccgtg gaatcgggca gcaactcacg tagatggtat tttgtcgatt
    18241 ttgacaggaa tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt gattgacgag
    18301 cttgtcagct taaaagaata agaatagatt tacggaaatt acttcccgta gtttataaaa
    18361 tcttgcctta ttgccgatag gctgtctttt gaacactatt tattcggaaa aaggggctat
    18421 gacatgcttt ttgtcatgcc ttctttttat gtgggctgct tttttagata ggagaaaata
    18481 aaaataacta tttaaacccg aacaaaaagg aataatgcat taaaaatcat tagattttac
    18541 aaaaaaataa gtaaaccacg ttgtcagttt cagaaagtag tgttagactg ttttcaatat
    18601 ttgaaacaac attttttatt caaatatggc aaactgcctt cacgtgcaaa acagttgcaa
    18661 gaaaatgaat ttacactatt attagtttta catacctact tatatatcgt cataatatgg
    18721 atgacagttt ctagccagta ccgtaaatgc tggactataa gtaaaagatt ggctatttca
    18781 cccttctttt cttatgataa agtggagggt gtttttcttt aacagaaaca tagtaggttt
    18841 ttgcacaaaa gcggttattt cggtattaat aaatgataaa ggagcaaata aagtgaaaga
    18901 attagttgaa cgtattcaaa aagacggacg tatattaggc gagggcgtat taaaagtcga
    18961 cagcttcgtc actcaccaag tagaccctgt attgatggaa caaatgggaa aacggtttgc
    19021 agaagtattt aaagaacaag gaatcacgaa agttgtgacc attgaagctt ctgggatcgc
    19081 accagcttta tttgctgcaa gagaattaga tgttccaatg atcttcgcta gaaaagcaaa
    19141 aagtttgacg atggatgaag aattattgac agcttctgtt tattctttta cgaaacaagt
    19201 gacaagtacg atttctattt ctcgtaaatt tttatcttca gaagacaaag tattgattat
    19261 cgacgatttc ttggcaaacg gacaagcggc aaaaggattg atcgaattat gccagcaagc
    19321 tggagcgcaa gtagaaggaa tcgggatcgt catcgaaaag tctttccaag atggacgtca
    19381 gttacttgaa gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt ccttgacgaa
    19441 tggacaagtt gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac acaaaatgga
    19501 aaagctgcgg ttttagggct gcagcatctg ctagcgatgt atgcaggagc agtagcggtg
    19561 cctcttttga tcggtacagg actaggcttt aatcaagaac agatgactta tttgatttca
    19621 attgatatct ttatgtgtgg attagcgaca ttattgcaat tgacagttaa ccgatttttc
    19681 ggaattggct tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc actgatcttg
    19741 atcggaacca atgaaggagt aggagctatc tacggatcta ttatcgcttc tgggatcttt
    19801 gttgtgttag tttctggtgt cttttctaaa attaaaaaac tattccctcc tatagtaaca
    19861 ggaacggtta tcaccgttat tggtttgact ttgattcctg tagcaatcga gaaaatgggt
    19921 ggaggtaatg caacagcagc tgattttggt gacaaaacaa atctgctact tgcatttgtg
    19981 acgattctcc taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg ttcaatcgcg
    20041 gtattgattg gtttagtcgg aggaagcatc ttagctgctt tcatgggaat gattgatctg
    20101 agcgcgattg gagatgcgcc gttgttccat gttccacaac ccttttattt tggtaaacca
    20161 actttcgacg tatggtcgat tttactaatg atcatcattt ccatggtcag catggtagaa
    20221 tcaacaggtg tatattttgc acttggtgac attactggga aaaatgttgg agaagaagaa
    20281 ttgaaaaagg gatatcgtgc agaaggactt gccgttatct taggcggtat tttcaataca
    20341 ttcccttata ctggcttttc tcaaaatgtc ggattggttc aactttcggg aatcaaaaca
    20401 agaaaaccca tctatttctc tgcattcttc ttgattgttt taggattatt ccctaaaatc
    20461 ggtgcgttag cacaaatcat ccctgagccg gtacttggtg gaggaatgtt agtcatgttc
    20521 gggatggtgg cagtccaagg aatccgtatg ctgttggaag ttgatttcag caatgataaa
    20581 aatctattga ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt tacgccgaca
    20641 ttattcagtc aaatgccgca aacattgcaa atgttcactg gaaatggcat cgtaatgagt
    20701 agcattacgg ctatcatatt gaatttgatc tttaatggat tgaaaaaaga tgaagaaata
    20761 ttggaagaag ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc agcgttttga
    20821 cctgattgtt agagaaacaa acggaaaaat agttcctcgt attttgccgt ttgtttgagc
    20881 taataccgag ggtcagctga ttgaacaccg ttgaaggaag aggtcgtgag cctgccgttc
    20941 atctccgagt cacaaggaac aatttcaaaa aaaagcttct catgtttgtt gaatattgtt
    21001 ccttgtgacc gaggagttgg caggagaaca ccgtttatcc ataaaaagga atgtgaccta
    21061 acttttgtca cattcctttt ttttatcttt aataaacacg aacaataaaa tgtttttctt
    21121 tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata tgaatgtaga
    21181 aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga gtacttcaga
    21241 ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt cttatgaaaa
    21301 aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac aaaacgctcg
    21361 aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc tgccgggtat
    21421 ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac gaacattaaa
    21481 tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg tagctactac
    21541 agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga tgctttccat
    21601 gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg ctaaaactgt
    21661 tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg atcgggatcg
    21721 tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg ggctttaaag
    21781 taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat tggcacattg
    21841 aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca gatgtcatta
    21901 cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc atgtcattta
    21961 ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa aaatcttttt
    22021 tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg acggatatcc
    22081 aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga ggcggctatg
    22141 atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc atggaccttt
    22201 taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag atttcgatca
    22261 tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa aacatccacc
    22321 acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat gtgatcgagg
    22381 aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt gttttagggg
    22441 tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct cctcgtcctc
    22501 acaattctgg acattattct attgaagctt gttctatgag tcagttcgat gcccatattc
    22561 gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat gcagtaatgg
    22621 tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat aagccagact
    22681 ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg ggacatatca
    22741 ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg aatatttggg
    22801 attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg aatatctgga
    22861 cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat gaagcttggg
    22921 cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat gcatcatttg
    22981 atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt gcgtttacac
    23041 gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt ctaacaagta
    23101 cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat attttacggg
    23161 aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac aaatatacag
    23221 tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga ttgaaacttg
    23281 ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg gcaaaaggag
    23341 tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg ccatttattg
    23401 aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatctctact caagttctcc
    23461 ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca agtattgaac
    23521 gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta gaagaatttt
    23581 ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg atcggttcag
    23641 aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc tatgaaaatg
    23701 tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc atcttgcctg
    23761 ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt aaaaatctaa
    23821 cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg atttatagcc
    23881 agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct tatgatcttg
    23941 ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg ctgttagaag
    24001 aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc tttgattatc
    24061 atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa taaagaagag
    24121 tgagttgcaa aattcaatta aaatagaata gcaaaaataa tcgagtagag gggaaagcca
    24181 actgaccttc cgcccctctc acaccaccgt acgtacggac ccgtatacgg cggttcaata
    24241 agttgagtgt acgaactggt acttcaagga gatgtcctca tatccttggc gggccagttc
    24301 tttgtctgtt aatgatcgat gaagaatatg acttttggag actgaccaaa taccttttct
    24361 tgtatttgac cattcatacg ctttcgcttt atcgatccct aaacgaatca gattttttct
    24421 tttggttctt ggattcttcc attgtttcca aatatattgt ctgattcttc gcttcaacca
    24481 accgtttaac tccctcatga attctttcat ctcaccaatt ccatagtagt tgatccaacc
    24541 agtcattagt tgcttgattt cttttaaaat aagttggata cttcttccac ggtttctttt
    24601 agtgagttgt ttgagcttgc ttttcacacg ctgttttgct tttgcgtgag ggcgaatttt
    24661 aatacctttc tttgttggca gaatgcagaa tccaagaaac tttcgtttca atggacttcc
    24721 taccgcactc ttttctcgat ttaccgtcaa ctttagttgt tcttcgagaa attgaatgga
    24781 tttcttcatc acacgctgtc ctgctcgttt gcttctaaca tagatattgc agtcatcggc
    24841 gtatcgaaca aatttatgtc ctagattttc tagcacttga tcaaattcgt tcaggtagat
    24901 gttacttaac aggggagaga gattgcctcc ttggggcgtt ccttcttcag ttgttgtgat
    24961 gagtccattt tccattacac cactcattaa gtattgacgg atcagtttta ggacttgttt
    25021 gtcctttact ttccgctcaa tgaaatacat cagcttatcg tgatttacgg tgtcaaagta
    25081 ggctttcata tcaatgtcca ccacatatcg gtaaccttgt tcataataag attttgcttt
    25141 cttcactgcg tcatgtgcgc tgcgatttgg gcgaaaccca taactattat ctgagaattc
    25201 tggttcaaat agcggttgta acacttgatt gattgcctgt tgaagcatac gatcaataac
    25261 cgtgggaatt cctagttttc ttacaccgcc gtttggtttc ggtatttcta ctcgcaaaac
    25321 aggctgaggt ttatagttcc cttggcgtaa attggatagt aattcctcgc ggtgctcttt
    25381 caagtaagga agcagttgat cgacggtcat cccatctgca ccagccgccc ctttatttct
    25441 gactacttga agatacgctt gattgagatt attccgatct agaattctcc caaacagatt
    25501 ttctacacca tcttgtttcc tctgttcacc gcaagaagtg ctgcgcactt catcataccc
    25561 ttcgtgttcc acactagctc tttgatgata gccaagtttt cttgttttct gcggtcgtcg
    25621 catcttctca cacctcctaa attattcaag attcttattg ttcagtcctt cgtaacggtc
    25681 agtgtcgtta ctactatgac ctcggctgac ttctcatgat tcaccatcat gtcgccatga
    25741 tcgttgtgct ggtttgtttt cattcaacgc ccacaacacc ctcatgagat ctcccccggt
    25801 aagagtgaaa actttcctcc catgtaactg ctagatttac tgtataggtt tcgggcagta
    25861 ttggactttg tcttgtgatg aagacttatc cgacctaatt cagccttgta tctagtttct
    25921 gttcgtcagt tcaggagttt gcgtccgact tccttcagcc acaacctcac gattgccgcc
    25981 ttgtctttcg ctaacagttc ctactgccaa gcctgtagtg gactttcacc accaagtttt
    26041 cacccatgca gggcgcacac caaaaaagaa gcagatccgt tagcggttct gcttctttta
    26101 gtttattata ttttcttatc aataatagga acctgatttt aaaagcggct acctctttac
    26161 cgtgcctctt tacatacaaa aaatagagga attaagcgta aatggcttgg ctattataac
    26221 taaaatgctg gttatggaga tgttgcagca agttttcgag aaaagcgtat tccatctctt
    26281 gtccattgaa taagatctgg atacgataat gatttttgaa acgcttctgt tcatgaatct
    26341 gactgattga aagttcgcct ttgtgttctg tctgataaag aaaagtcctt ttacggcatt
    26401 cagcacagcg cttggttgct cggcggattt ttcggaatgt ataaaaacta gtatctttat
    26461 tgcataaata aacgtctttg atcgacagca aatcattata tagacaggaa cgttcttcag
    26521 ctgttaattc tgataaaaaa agctgacctt gttttattgc atcttccatc ggcaagtgcc
    26581 tcctcgaacc cattatccaa atttttagtt aattctaata taccatcatt aagtataaat
    26641 gtaaacgctt gttgatggta taagattttc gtttctgata aaacacgaac attaaaagaa
    26701 tctaatgcct taaatgtaca tatttttgtt gatttataaa aagatgattg ctaaaattta
    26761 aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa aaggagagac
    26821 gtacatggag aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat atcttacttc
    26881 tgatgagaac ttgatttgga tagaatattt agatcaagca actgcgttga atggcgcgag
    26941 gaaagaccag ataagtggaa aaggagaatt gaataatcaa atcacaagtg caattttccg
    27001 ttatctgact caaagaggaa taaaaaatca ttttattgaa caattgtcta aaaccgaaca
    27061 attagcaaaa aaagtacaaa ttatccctct ggaagtggtt atccgaaata ttgcagcagg
    27121 aagtttctca aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac caatcattga
    27181 attctactat aaggatgacc agttggatga tccatttatc aatgaagagc atatctattt
    27241 cttacaactt gcaaagccgg aagaaatcag tgagctgaaa gcccaagcgt tggctattaa
    27301 tgaaattttg accgaattat ttgctaaggt cggtttggtt ttagtagatt tcaagctaga
    27361 atttggccgg tcgaatggac aaattttgct tgcggatgaa gtaacaccgg atacttgccg
    27421 attatgggat gaacgtacga atgaacatat ggacaaagac gtttaccgca gagatctagg
    27481 tgatttgatc ccagtctatc aagaagtata taaccgtcta tcggaactaa ctgaagaaaa
    27541 ggagcgagta tgatgtattt cgtaaaagta tatgtaacct ataaagattc agtgttagat
    27601 cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac aatcgaagat
    27661 atccgaatag gaaaatactt tgagatacaa cttgctgatg cagaagaacc aatcgataca
    27721 gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa atatggaaac ataccgatat
    27781 gaaatcaaaa aaatggagga agtataacat gaaatttgcg gtcatcgttt ttccaggttc
    27841 aaattgtgac gcagatatgt tatgggcagt tcgcgagatc atgggcgctg atgccgaatt
    27901 tgtacggcat gatgagtcct ctttagaagg atttgatggg gttttactgc cgggaggatt
    27961 ctcctatggt gactatttga gatgcggtgc gattgctcgg ttttctacaa tcatggaaga
    28021 agtgattcgt tttgctgaag aagggaaacc agtattcggt acatgcaatg gattccagat
    28081 attgacagaa gcaggacttt tgccaggtgt actgagacga aatgagtctt tgcattttat
    28141 ttgcaaaact gtaccattga aagtcaaccc caagacggcc tttacttcag agtatgaagc
    28201 cgacgaaata atctatcttc cagttgctca tggggaagga aattatttct gtgacgaaga
    28261 aacacttcaa gaactaaaaa aaaataatca gattgccttt acttataatg acaacatcaa
    28321 cggcagcgtg gaaaacattg cagggattac gaacaaagca gggaatgtgt taggaatgat
    28381 gcctcatcca gaacgtgcag tagaagactt gctaggatca gaagatggga aacggttctt
    28441 tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc
    28501 gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa tggggattga ctgatgaaga
    28561 atatcgaatg atcgaagaag atatcttagg aagaatgcct aattatacgg aaactggact
    28621 gttttctgtt atgtggagcg aacattgttc ctataaaaat tctaaaccgg ttctacgcaa
    28681 atttcctact agtggacctc atgtgttgca aggccctgga gaaggcgcag gcatcgtcga
    28741 catcggtgat ggactagcag ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt
    28801 ggagccttat gaaggagctg cgacaggagt tggcggaatc atcagagata ttttcagcat
    28861 gggggctcgt ccaattgcgt tgctagattc attgaggttc ggcgaattag ataatccaag
    28921 aacgaaatat ttactagaag aagtagttgc aggtatcggc ggatacggca attgtatcgg
    28981 cataccgact gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa acccattagt
    29041 caatgcaatg tgcgtaggtt tgattgaaca caaacatatc cagaaagggc aagccgctgg
    29101 cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta tccacggagc
    29161 aacttttgcc tcagaagaat ttaatcaaga agaagaacag caacgttctg ctgtacaagt
    29221 aggcgatcct tttatggaaa agctgctgct tgaagcgtgt ttggaattaa tcttgcatca
    29281 ttcggattct ctaataggta tacaggatat gggagcagca ggacttgttt catctagtgc
    29341 ggaaatggct tcaaaagctg gctcaggact tatattgaca ttagatgaag tcccacaaag
    29401 agaaactggg atgacacctt atgaaatgat gttatcggaa tcacaagaac ggatgttgat
    29461 ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta tttcagaagt atgatctaga
    29521 tgcagtcact atcggtaaag taacagacga cgggcaatat cggctatatc atcaaggaga
    29581 agaagtagca aatctgccag tcgatgcatt agctgaagat gcaccagttt atcataaaga
    29641 aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta gctccttatc agccagttat
    29701 cgaagaacct ggacaagtat tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg
    29761 ttcaatctac gaaacttatg attcacaagt acaaaccaat acagttgtcc gtccaggaag
    29821 cgatgctgct gtgatgaggg ttagagggac gaataaagcg ttagctatga ccacagactg
    29881 caacgcgcgc tatctgtatt tggatccaaa aatcggagga caaatagcag tggcagaagc
    29941 tgctagaaat attattgcta gcggtggtaa gcccttggcg atcaccgact gcctgaacta
    30001 tggttctcct gataaaccag aagttttttg ggaattatcg acttcagctg acggaattgc
    30061 tgctgcgtgt gaaacgttag gaactcctgt tatttcagga aatgtctctt tatataatga
    30121 aacggatggt caagcaatct atccaacacc aatgattggt atggtcggat tgattgagga
    30181 tcataaacat atcactactc aggaattcaa aaaatcaggg gatctaatct atatattagg
    30241 taagacgttt gcagattttg atggcagtga attacaaaag atgcagcttg gaagaatcga
    30301 aggtgtgatc cgaaactttg atctatccat tgaaaagaga aaccaagagt tagtattgac
    30361 agccatccaa aacggattga ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat
    30421 tgcactggca gaatcagctt tcaaacatca gctagggata tctgtacagt ttgaattgtc
    30481 ctctgctcaa ttatttgcag aaactcaatc aagatttgtg ttgacagtcg caccggaaaa
    30541 taagacacga ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta aggtgacgga
    30601 tgaagcgata atcgaaattt cagcaactga cggtcagatc aaaatagaaa ctgcagtagc
    30661 aagaaagtgc tgggaggatg caatcgtatg tcttatgaag tgaagagttt aaatgaagaa
    30721 tgcgggatct tcggtgtttg gggtcatcca gacgccgcta gtgtggcata cttcgggctc
    30781 catagtcttc agcatcgagg acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag
    30841 cttaaggggc atcgagattt ggggctgctt tctgaggtat tcaaagatca aagaaaattg
    30901 cagcagttaa caggagaagc tgcaatcgga catgtacgtt atgcaacagc aggcaatgga
    30961 agtgtggaca atattcagcc ttttctattc aaatttcatg atcagtcgat cggcttagcg
    31021 cataatggta acttgatcaa tgctcgttcc cttagaaaag agttagagga aaatggggcg
    31081 atcttccatt ctaactccga tacggagata ttgatgcatt tgatccgacg aagtgaagaa
    31141 acagattttt taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat
    31201 ttattaatga cagaaacggc aatgattgcc gctttggacc caaatggttt tcgtccatta
    31261 gcaatcggac aaatgaaaaa cggagcatac gtgattgcct cggaaacttg tgcattagaa
    31321 gttattgggg cacgttttat aagagatgtc cagcctggtg agatagtgat catcaatgac
    31381 gaaggaatcc agattgattg ctttacgaag gacacgcagc tttctatttg ttcgatggaa
    31441 tatatctatt ttgccagacc agattcaaac atcgcaggga tcaatgtgca tactgcacgg
    31501 aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc
    31561 gtacctaatt cttctttgtc agccgcaagc ggttatgcgg aagctagtgg aatcccttat
    31621 gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc aacgcctgag
    31681 ctaagagagc aaggtgttcg aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa
    31741 aaggtgatta tggtcgatga ttcaattgtt cggggtacga caagcagacg gatcgtccat
    31801 cttttgaaag aagcaggggc aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa
    31861 tatccatgtt tttacgggat tgatattcaa acgcgtaaag aactcattgc ggcaaatcac
    31921 acgattgatg aaatcaaaga atgtatcggt gcagattctt taggtttttt aagtgaagag
    31981 ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt attcaggatt gtgtatggct
    32041 tatttcaacg gagattatcc gacgccgtta tatgactatg aagaaaaata tcaggaatca
    32101 ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga agggagtata tgaattcaat
    32161 ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg
    32221 gatcaagaag catgtcaaaa aaacggaacg tttaggtatg atgggcgcta taggaggatt
    32281 cggcggttgt tttgatttat cggtctttca attaaaagag ccagtccttg tctcggggac
    32341 cgatggcgtg gggacgaaat tagcagtggc aatcgaagca gacaaacacg atacaatcgg
    32401 gatcgactgc gtagcgatgt gtgtcaatga tatcttagca caaggcgcag agccgttata
    32461 tttcttagac tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa
    32521 aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg attggcggag aaacagctga
    32581 aatgcctggt atgtactcag gtaatgatta tgatctagcc ggatttgctg tagggatagc
    32641 tgaaaaatct caattattga caggagaaac gatcaaccca ggagatgtgt tgcttggaat
    32701 agcatctagc gggattcatt caaatgggta ctctcttgtt cgcaaaattt tctttgagca
    32761 gcagcagttt tctttatcat caaaactgcc agaattgaaa aaaacacttg gcgaagaatt
    32821 gcttgagcca acgaaaatct atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat
    32881 aaaaggagct gcacatatca ctggtggagg gtttgtagaa aatatcccgc gaatgttacc
    32941 agaatctatg gctgcacaaa tcaaacttgg ctcatggccg attcttccaa tattcggctg
    33001 tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat gaaatcttca atatgggcat
    33061 cggtatggtc ctggcagtcc aagaatcgaa agtaaaagaa atagtacacg aaattgagca
    33121 agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt
    33181 gtttatagga gaagaacgat gagaatcgct gtttttgctt ctggaaacgg aagcaacttt
    33241 caagcgctag ccgattatct atcgaaaaaa ggattggaat cttcgattga ctggttgttt
    33301 tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt accggcagat
    33361 tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat tttgcataaa
    33421 ttgaaagaaa aaaagatcga tttgatcgta ttagctggat atatgcgaat cattggaccg
    33481 gtattgctga aaaattatga taaaagaatc atcaatatcc atccgtctct tttacctgcc
    33541 ttccctggtt tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc
    33601 acaattcatt atatcgacca aggtgtagat actggaccaa tcatccggca ggaaaaagtg
    33661 agaattgaac aggaggacac gttcgattct ttagaagaaa aaatccatag agtagagcat
    33721 cgaatctatc cagaagtgat tagtgaaatt attgaaaatg gaggctatct gaaatgacaa
    33781 gagcattgat cagtgtatca gataaaactg ggattgtagc attcgcccaa ggtttgattg
    33841 aacaaggagt agaaatcatt tcaacaggag gaacaaagca tgcgttggat aaagctggga
    33901 tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga aatgatggat ggccgtgtca
    33961 aaacacttca tcctaaaatc catggtggtc ttttaggaag acgcgatctc cctagccata
    34021 tggaggcaat ggaaacggaa aatatccaac caatcgactt tgtttgtgtc aatttatatc
    34081 ctttcaaaga aacgattatg aaaccagatg tcgaaatagc agaagctatt gaaaacattg
    34141 atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg
    34201 ttgttgatcc atctgactat gaagaagtac tttcagaatt acgtcagtat gggcatactt
    34261 cactttcttt cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg gcatatgatg
    34321 cactgatcgc tcaatactta acggattggg tggaagaaaa agaaccggaa aaattgacac
    34381 ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct
    34441 tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga
    34501 aagaactttc ttataataat atccgagatg cagatgctgc gctacggatc atgaaggaat
    34561 atgacgagcc aacggtagtc gccttgaaac acatgaatcc ttgtggtatt gggacagcat
    34621 ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca atctttggtg
    34681 ggatcatcgt tttgaatcga gaagtagatc tgccaacagc agaacagatg cataagctgt
    34741 ttctagaaat catcattgcc ccttcttata cacaagaagc tttggaatta ctgcaaaaaa
    34801 agaaaaacat ccgattactg acagtagact ttgcgcagga agaaaatcaa aatcgagaag
    34861 aaacggtctc agtcttaggt ggacttcttg tacaagatca agatctggca atagaaaatg
    34921 aagaagagtg gaaagtcgtg acaaaacgta agccgactcc agcagaagca aaagcattag
    34981 cgtttgcttg gagagctgtc aaacatgtga aatctaatgc gatcgtactg gcaaacgaac
    35041 gtcagacagt aggaatcgga gccgggcaga tgaatcgagt cggttcagta aaaattgcga
    35101 ttgaacaagc gcaagagaaa atggaaggtg cagttttagc tagcgatgct tattttccaa
    35161 tggatgattc ggttgaatat gcagccaaac atggtataaa agcaatcatt cagccaggag
    35221 gaagcatcaa agaccaagat tctattgata tggcgaataa atacgatcta gcaatggtct
    35281 ttactgatgt tcggcatttt agacattaga gaggagtcgg ataaatgaac attttggtga
    35341 tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact tttagccagt ccgaaggtgg
    35401 atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg
    35461 ctatcagtga gcgagatcat tcagcgttaa tccattttgt taagaaagag gctattgatt
    35521 ggagtatcgt tggtcctgaa gttcctttgt taaatgggat cgtcgatgat tttatggcgg
    35581 aaggattgaa aatcttcggt ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt
    35641 ttgctaaaca attaatggat acttatcaaa tcccgacagc taaatccaga agttttgaat
    35701 gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg
    35761 acggcttagc agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg
    35821 cggcagaaca aatgttgaac cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag
    35881 aagagttttt ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat accaaggtgt
    35941 atccgatggt catttcgcaa gatcataagc gggcatttga taacgataaa gggccaaata
    36001 caggcggaat gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag
    36061 ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa agaactagga cgctctttca
    36121 cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg accaaaagtg atcgaattca
    36181 atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc tcggttgaag agtgactttg
    36241 cgcaagtaat cgatgattta ttagagaatc gtacagtaga gttaaagtgg cagcaagaag
    36301 gatacaatgt aggcgttgtg gttgcagctg ccggttaccc cgaagaatat gagacaggaa
    36361 tggttctacc agattttctg gaagaagagc tttctgttta ctatgctgga gtttcctcaa
    36421 ataacggcaa actagttgga gcaggaggac gtctttattt agtagaagca tacggtgaga
    36481 cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat ggataaaacg acagatggaa
    36541 cattttatcg tacagatatt ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa
    36601 tcagctgctt ccatttataa aaaatacaac aaacataatc ggaagcgccg attttagtga
    36661 agccttttca taaagagtgt ggtacacttt tgttgtgtat ataaataagg aggagttcag
    36721 atgaaaaaaa ttcaattagg tacttctgat cagaaagcag ctgcagtgat cttaggctgt
    36781 atgcgtatca acaatgcgga agatccagta aaagtgattg agacagctta tgaaaacggc
    36841 attgattttt ttgaccatgc agatatttat ggaaacggtg aatgtgaaga aatctttgca
    36901 gatgctctag caaaaacatc aatcaaacga gaagatcttt ttattcaaac aaaatgtgga
    36961 atcgttccag gtgtgatgtt tgatttttct aaaaaacata tcattgaatc tgtagaaaaa
    37021 agtttgaccc gtctgaaaat ggattatgtg gatgctttac ttcttcatcg tccagataca
    37081 ttggtagaac cagaggaagt agctgaagcg tttgatgaat tggaacggtc aggaaaagtt
    37141 cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt
    37201 cgtcaaccat tagtagctaa tcagctgcaa tttggcttga aacatactgg aatgatcgat
    37261 caagggattc atgtcaatat gacagatgaa gcaagtatcg atcatgacgg tagtgtactt
    37321 gattattccc gattgcatga tatgacgatc caagcatggt caccatacca gtatggattt
    37381 tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac attagaagaa
    37441 ctagcagcta aatatcaaac gacaccaact ggcttggctt ctgcttggat tttacgccat
    37501 ccagccaata tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct
    37561 caagcttcag aaattaatat gtctcgggaa gattggtacg aattgtaccg tgctgctgga
    37621 aacattttac cttaaagatt gtcataagca ggtcaaaagg gctataaatt aaaaagacag
    37681 aataacttga aagctgtttt cagcttcgag atattctgtc tttttttatt gcaaatggaa
    37741 agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga aaagctgtgt
    37801 ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga aatagttagg
    37861 gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt gctgcaataa
    37921 tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt ggttaaacga
    37981 ttgagaaaaa atcgtactga aatgagaggg acttaatgca acttgttgag cgacccaagt
    38041 taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat ggttcaaaat
    38101 tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag aaatgaacat
    38161 ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga
    38221 tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat gctggagtag
    38281 cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt
    38341 caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat caagtgagag
    38401 gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat cctcatagct
    38461 gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga tctcaaaaga
    38521 tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa aattattttt
    38581 ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg tctcgatcac
    38641 attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa
    38701 gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat gagcgtaatc
    38761 ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt tccctgctaa
    38821 agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg agcctttgaa
    38881 caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt
    38941 ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt tcaattcgct
    39001 acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc ctttcatata
    39061 atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta gtttccttgc
    39121 ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca gaatgatgtc
    39181 gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt cgccgacata
    39241 agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat tgcgaatgag
    39301 gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt gttttgggag
    39361 atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca atcgtgatag
    39421 gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa agtccgtagc
    39481 cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct gccagttttt
    39541 ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca tcaattttgc
    39601 atgtggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg tatacagcat
    39661 tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt gtttctggta
    39721 tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact agataatcat
    39781 tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt aaagacgtac
    39841 gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag tcttgttcga
    39901 tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc tgagcttgca
    39961 atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc cgtaaagact
    40021 ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat tccagtgttt
    40081 ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac atctcgatca
    40141 gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt ttcgcataga
    40201 aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc gagcggacaa
    40261 gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct tgtaagatat
    40321 ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg gtatccataa
    40381 aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt gtatcgatcg
    40441 tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta tttttgatct
    40501 ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca tctacggtta
    40561 cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa
    40621 ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat
    40681 ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct ttgtttgctt
    40741 gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag tcaataattt
    40801 ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat aaaaatatct
    40861 ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt attggctaca
    40921 taccagccga catcaggttt acactcgact acacaattga cgattcctga gcgtagcttc
    40981 tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc aggtttgatt
    41041 gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg gctgtataat
    41101 acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc ttttcctttc
    41161 atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt atggtcgatg
    41221 gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt
    41281 agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat ttcctgtttc
    41341 tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc tgctttgaaa
    41401 ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca
    41461 agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca cccggattct
    41521 tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg tatatcttgg
    41581 atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc ttctttttca
    41641 agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt tcctgattgc
    41701 gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca ccccatcatt
    41761 tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt aaatcactcc
    41821 atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc
    41881 aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa
    41941 tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc tatactcggt
    42001 gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt gttgggacta
    42061 tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga tctgcagatg
    42121 atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga tggatcgctg
    42181 atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat cttcaatatg
    42241 gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca aaaggggtag
    42301 atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta gctaaagcaa
    42361 aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca gaaaatgtcg
    42421 attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct tatattgaag
    42481 agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt ggtggctcgc
    42541 ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta caaccatata
    42601 tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa atcgctacgc
    42661 ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc gcaaattata
    42721 ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta ggaatcattt
    42781 cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg attactggac
    42841 aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca caaacgatct
    42901 ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca atcagtgatg
    42961 gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa actgtcccta
    43021 cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag ctgatcgaca
    43081 ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag gaactaaaga
    43141 gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa tggcagatat
    43201 caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg
    43261 ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt
    43321 gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg gaaagctgtg
    43381 tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc atcaagaatt
    43441 agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg aacaagcaga
    43501 aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc tgaataaagt
    43561 cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc aacagcaact
    43621 agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg atgagcctac
    43681 cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga
    43741 gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg atatcgcaga
    43801 tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg aaatgatcag
    43861 cgaagagcat attattcgcg gaatggtggg cagagattta gttgatcgtt atcctgaaag
    43921 aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct atcaccctct
    43981 tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag gagagattgt
    44041 aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc tatttggaca
    44101 ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga tatcagttaa
    44161 gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc gaaaatcttt
    44221 aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt tggataaaat
    44281 aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa atttccggaa
    44341 aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta gtggtgggaa
    44401 tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc tttttttgga
    44461 tgagccaacc cgtgggatag atgttggggc aaaatatgag atctacacga ttatcgaaga
    44521 aatggcagca aatggcaaag ctgtctgtct tatctcttct gaacttcctg aaatattagg
    44581 gatgtgtgat cgaatctata cgatgaacga aggacgtatt acaggagaag tctcgcgaga
    44641 agatgcagat caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg
    44701 aacaggcaaa aaatcttacg gataaaaaag agaaaaaact aaatttgaaa agtagagcag
    44761 ctgatctttt tggtcgctac agcatggtga tcattttagc gggactactt attgcgtttc
    44821 aattgatgac ggatggtatt ttctggcgtc cattgaatat aactaacctt gttctgcaaa
    44881 atagccatat attagtacta gctgcaggga tgttattagt tgtgctctta ggtcatgtgg
    44941 atttgtcggt agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg atgatggtca
    45001 atcgccaaat gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc gggctgatcg
    45061 gcgcatggca aggtttttgg gtagcttatg cagggatacc ggcgtttatc gtgactttag
    45121 caggattgct gatgtttcgt ggactgacac aagttgttct aggaggccaa tctctagcac
    45181 cttttccagc tgttttccaa aaaatgtcta cgggttattt accagactta agcgaaggaa
    45241 gcagccttca cttcttgaca ttgattttag gaatgatcat cacgatcgtg ttagttgttg
    45301 gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa tctccaacag
    45361 ctttcttggt aaaggcggta tttacagcag tcgttgtact aggaataact tatatctttg
    45421 cctcttatca agggtatccg attattttga tcattcttgg tttgatcgtt gcagcatatg
    45481 ggtttttgac aaataaaaca gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa
    45541 aagcggctga actatcaggg atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca
    45601 tgggagtcat ggcagcttta gctggattag ttttagctgc tcgattgaat gcggcaacac
    45661 ctcaagcagg tacatcgatg gaactggatg caatggcggc agtatatttt ggtggtgctt
    45721 cgacttccgg cggcattgga acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg
    45781 tattgaacaa tggcatgtcg attttaggcg tcggggtaga ctggcagcag gcaatcaaag
    45841 gattgatctt gcttttagcc gttgtgctag acttgtataa taagaaaaaa cgaacaacgt
    45901 aggagaacgc cggttattca gaaaaaagac tgtgacaaga catttgtcac agtctttttt
    45961 tcgctaaaag cgaacattca ttgtttcgtt tgtatagact aaaaataata tatgtttttt
    46021 taccattcgt ataaattctc tttttgctct tgattttttt gcttaattga gttaaattac
    46081 atagggcgct aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc tggatgtgaa
    46141 catcaggagg ataatgtaat gaaagtattt gattacgaag atattcaatt aattcctaat
    46201 aaatgtatcg taaatagccg ttctgaatgt gatacaacgg tcacattagg taaacataca
    46261 ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta ttgatgaaac aatcgcagaa
    46321 ttcttagcgg aaaatggtta tttctatatc atgcatcggt ttgatgaagc agctcgaatt
    46381 ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag
    46441 aaagaggaat attcctttat agagaagttg gcagaagaat cgctaaatcc tgattatatc
    46501 actatcgaca tcgcgcatgg acatgctaac tcagttattg atatgattca gcatattaaa
    46561 aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt
    46621 gaattggaaa atgcgggcgc agatgcaaca aaagtcggga ttggtccagg aaaagtatgt
    46681 attacaaaaa tcaagacagg ctttgggact ggaggctggc aactggcagc tcttagatgg
    46741 tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg ggatcagaac acatggcgat
    46801 attgcaaaat ctgtccgttt tggtgcgaca atggtcatga ttggttcatt gtttgccggt
    46861 cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt
    46921 ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg
    46981 atccaacaca aaggatcttt gaaagataca ctagtggaaa tgcaacaaga ccttcaatca
    47041 tctatttctt atgctggagg aagagatctg gaagccattc gaaaagttga ctatgtcatc
    47101 gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa taaataaaat attaggatgt
    47161 tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca aacaaaaata gttctttata
    47221 tgttttgttt ggctggaatt attatcgcag atacaagctt ttgaacatca ttattcagaa
    47281 taagggactg tgataagatt tttgtcacag tcccttatgt tttgcaaaaa aagtttttct
    47341 tttttctcgt aaattcggta aaatcaaaag aaaaaataga caatagaaaa gagaagctta
    47401 tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg tcaaattatg gaacagatta
    47461 tgagagaaat cgaaaacgga aggatcaatc ctggaagtca acttgaatca gaacgaaaac
    47521 tagggcaact ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa gagcttaggg
    47581 agcttggggt actaacgagc aaacgtggca gcggatggtt tgttaatcag actgaatggg
    47641 ggaaattcgc tgttccaaga atcgattggc ggcaaatgat ctctcctcgt tatgagcaga
    47701 cggactggta tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca tcgtttcttg
    47761 atttatacgc tagcgagatg ccagatgagc ttttgccgaa ttttgagttt ccttcttatt
    47821 ctcttgaaga aatcttagcg gaagaaaaag aaatggatct tttaggctat actccgttac
    47881 gtaacaaaat caaacggtat ttggaaagta aatttgaatt ggcattttcc cccaaccagc
    47941 ttcttgtgac tggtggcggt cagcaagcaa tctttttgat tttacaaacg attctttcag
    48001 ctggagaggg gatagctgta gagtcacctt ctttttttta ccgattacct cttttcaaag
    48061 cttcaggaac gcgtttattt gggataccga tggatgaaga agggatcgac ttagaaaaac
    48121 tgaaagaatc gatccataaa aataaattga aagcagtgct tatcaacccg aattttcaaa
    48181 accctacagg aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac
    48241 agtatcggct tccaattatt gaagacgatg tatttacgga tctttcattt ctaaaccaag
    48301 aagaagtcct tcctatacga acgattgatc cggataatgt gttatacgtc ggttcgttat
    48361 ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat aggacctgag acttttatct
    48421 cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt acacaaatca
    48481 cagtggctaa tgtatttaat gaagcaattg atttgaaaat gcaagatgta agaaaaaagt
    48541 tgaaagaaaa aagccgactg ataagcgctt ggggagattc acaatcgttt ttcaagcttc
    48601 attcaatttt aggtggttac tatgcctgga ttacttggga tgggaaaaaa ctagaccccg
    48661 aactagcaga aaaagtagta gatatgggtc ttggaattgc accaagtcta ctttttgggc
    48721 aagatataaa tggaatcaga atcaattaca gtaggctgga tgaaaaccaa ataccgttgt
    48781 tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga ataatttcac aaagtaaata
    48841 gattaaagaa tataacagct tggatttaaa tttcaagctg tttctttttt ttcggaaaaa
    48901 gaatacgata taaagaaact aagaaaaaac cattatttgt acgtccgttc acgatgtgta
    48961 aataaaataa atgtttgcaa caaaactgta taacaccaac gtttttaaat agaaacagat
    49021 ggttgtaagt ttgattagaa aatagaaaaa atgcagatga aatataaaac gctttcctta
    49081 ttgtgatttt attcactaat gctgttaata tgcggattgg cataatttcc tttcatcgtg
    49141 aaaatttgtg cttttagctt gtatttctca taaatccatg ataatttatt aatgaagacg
    49201 gttaaatagt gatacagttt tattcactgt ttcaatcgca gaatgaagag attttcttat
    49261 agatgagaga ggaatgtgtg acaaatggca aacaaaaaag caccgatcga cttccaagca
    49321 ctgctagatg aagtcaatgc agatttccca gtatatcaag cacttgatca agatggaaac
    49381 gtagttaaca aagatttagt acctgattta tccgatgatg aactcgttga attaatgact
    49441 catatggtat ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg ccaaggacgc
    49501 ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc agctggctag tgcttacgca
    49561 tttgataaag aagatgttct tcttccaggt tatcgggatg tacctcaact agtaaaacat
    49621 ggtttacctt tatctcaagc ttttttatgg tctcgaggac atgctgcagg taattcttat
    49681 ccggaagaat tgaaagctct accaccacaa attatcatcg gtgcacaata tgtacaagca
    49741 gccggtgttg cattaggttt gaagaaacga aacaagaaaa atgtcgtctt cacttatact
    49801 ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac
    49861 catgcaaatg ccgtatttta tatccaaaac aacgggtatg cgatctctac acctcgtgaa
    49921 aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg cagctgggat tcctggtatt
    49981 caagtcgatg gaatggatcc gcttgcagta tatactgtat ctaaaatggc acgggaatgg
    50041 gcgatcagtg gaaatggacc agtcctaatc gaaacactga cttatcgtta tggaccacat
    50101 acactatctg gtgacgatcc aacccgctac cgtacaaaag acacagatga cgattggcag
    50161 aaaaaagatc cattgatccg cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa
    50221 gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa
    50281 gccgacaaag taccaaaaca aaaggtttca gatttcttga aaaacatgtt cgaagtatct
    50341 ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa ataatcatgg
    50401 cacaaaaaac aatgatccaa gcaatcacag atgccttagc acttgaacta gaaaatgacg
    50461 agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg aggagttttc cgggcaactg
    50521 aaggattgca ggaaaagttc ggagaagacc gagtattcga tactccttta gctgaatctg
    50581 gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc
    50641 aattctttgg ttttattttc gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa
    50701 gatatcgaat gagcggcact agaaatctac ccatcacaat tcgttcgcca tttggtggcg
    50761 gtgttcatac accagaattg cattcggata atttagaagg tttgatcgct cagtctcctg
    50821 gtattcgagt agtgattcct tccaaccctt atgatgcaaa aggactattg atttcttcta
    50881 ttagaagtaa cgatccggtt gttttcctag aacacatgaa actatatcgc tcattccgag
    50941 aagaagtgcc ggatgaagct tacgaagttc ctttggataa agcagcagtt actcgtgagg
    51001 gaacagatgt atctattatc acatacggag caatggtgcg tgaagcaatc aaagcagcag
    51061 acaatcttgc aaaagaaaat atatctgttg aaatcattga tcttcgtaca gttgcgccat
    51121 tagatgtaga aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag
    51181 aagcacaacg acaagcgggc gttgccgcgc aagtagtttc tgagatctca gaacgcgcta
    51241 tcctttcatt agaagcccca atcggacgag tttctgcacc tgatacagtc ttcccatttg
    51301 gacaagcaga aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa
    51361 tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa tttaaattgc
    51421 cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag
    51481 atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa
    51541 tcccgtcacc tgttacagga acggttaaaa atgtgattgt tccagaaggt accgtagcaa
    51601 atgttgggga tgtattagta gaaatcgatg caccaggaca cgaagacaat gaaggggaca
    51661 gtggtgtagc cgcagaatcc caaactccag ctaaaccggc tgctgaaccc actgttgata
    51721 cagaatcagc tggatcgtca agcgaaggag tcttccaatt taagttaccg gatatcggtg
    51781 aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg
    51841 aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg
    51901 tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat gttggggatg
    51961 tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt gcgccatcag
    52021 ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt gaagctgccg
    52081 atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt gaaaaagatg
    52141 tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa gaggacatcg
    52201 aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg
    52261 cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca gcaaaaccat
    52321 tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta
    52381 tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg catgatgaag
    52441 tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct gctgcaaatg
    52501 gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact gttaagaaat
    52561 acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat aaacattact
    52621 acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat gtgaaagatg
    52681 cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca aaattagctc
    52741 atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc agcaatatcg
    52801 gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta gctattttag
    52861 gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc gttgtcggtc
    52921 gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca actgcgcaac
    52981 aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg atggaaggat
    53041 gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt aattggtgcg
    53101 ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc
    53161 attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca
    53221 ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt tggagtaact
    53281 agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc
    53341 catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga aacaatcgaa
    53401 ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa
    53461 acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga gattccagga
    53521 tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa agaagtacct
    53581 aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg agcgtacgca
    53641 aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc aacttacgaa
    53701 aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg
    53761 aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa atatgcagtg
    53821 gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg
    53881 aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg aggattgatc
    53941 acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg tgatatcgtt
    54001 ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc
    54061 tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt cactgatcct
    54121 gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa
    54181 gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa aacagaagga
    54241 ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca aatcggtgga
    54301 gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg aatgaatgct
    54361 gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt catggatgct
    54421 tcggaattag cattaggttt accaatccat atttgatttg aataatacag gcgaaacgca
    54481 actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt atgtaaactt
    54541 aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa gaggaatatg
    54601 aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat tggacaacag
    54661 acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat gaaaaaggga
    54721 taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc aaatccattg
    54781 gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct ttatatcgta
    54841 cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg tgagataaat
    54901 ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc
    54961 ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag taacaaatgt
    55021 cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc cagaggcgaa
    55081 aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa gagtctttat
    55141 catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt tttgtattat
    55201 gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg tgatgagaaa
    55261 tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc cattgcctag
    55321 accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc ttttcggtca
    55381 taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta tgtacggatg
    55441 cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata tttcaaacct
    55501 tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta tggtaacaag
    55561 cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag cagggacttc
    55621 ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa
    55681 tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa atgaaaacat
    55741 tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa tttgccctgt
    55801 cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga tatccgtaac
    55861 gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga actattaaaa
    55921 caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg
    55981 ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga ctataaaggt
    56041 attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga agtagaacgg
    56101 atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct
    56161 caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat cgttgtcgta
    56221 aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta
    56281 ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta tgcatctgca
    56341 ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac catggcacct
    56401 atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga cgaaccatta
    56461 caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg aatcggacgt
    56521 gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt agacggaaca
    56581 gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg tctagagatt
    56641 caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat ctttgtcggt
    56701 gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat tgacgaacca
    56761 acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga aggtaaattc
    56821 gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga tgtatcctta
    56881 cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg agaattgcat
    56941 ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt atctcgtcca
    57001 gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc
    57061 gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa
    57121 atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt aacacctgcc
    57181 cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta cggaatcatg
    57241 aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg acgtcaccaa
    57301 ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat gagtatcgaa
    57361 gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat gattatcggg
    57421 gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca aatgacaaac
    57481 gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg
    57541 gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc agaaagcatt
    57601 cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa
    57661 taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct ggtttctttt
    57721 tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa ttcagaaaat
    57781 acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt tttgtttcat
    57841 aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa
    57901 tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt ggctatgatg
    57961 agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa aatgtattag
    58021 gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct ttactattag
    58081 atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag gtggacgcgg
    58141 cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt tacgaaacag
    58201 ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc gctcctgaac
    58261 gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta
    58321 ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt tatttacaga
    58381 tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg acagctacag
    58441 cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa aacaaccata
    58501 ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat caaaaaaaag
    58561 aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc atttatgcag
    58621 caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat ttttccgtgg
    58681 gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa gcctttttat
    58741 acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata aataaaagca
    58801 atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac tatcaggaag
    58861 ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc tctcctcaag
    58921 atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg caaaagcaaa
    58981 aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc tgtcttcagc
    59041 aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt
    59101 tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt tcttgcttaa
    59161 aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag
    59221 aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg atgaaaaacc
    59281 aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc tatcttctga
    59341 ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa gtattgaaag
    59401 gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg gatgaagaaa
    59461 ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg
    59521 ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga atacgtccat
    59581 caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt gataaatatg
    59641 gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat aagtagaaag
    59701 aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt gctccttatg
    59761 aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga tggattcttt
    59821 gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca aacgattaat
    59881 ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag aagtggtaga
    59941 tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag gagtaatcgg
    60001 aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc agttatatag
    60061 tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat agacttgcct
    60121 aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta tttaatttta
    60181 tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt agaaaacgga
    60241 caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag cttgattgct
    60301 atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt gatcatggca
    60361 gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa ggagatcaat
    60421 ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga atacctaaaa
    60481 atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa
    60541 gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac tgcattagtc
    60601 gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt ggttctgctt
    60661 ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg cttttgtcta
    60721 agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc cggacgttta
    60781 cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga attaaataaa
    60841 gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt tggcagggga
    60901 ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga ttttatttat
    60961 gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc gttgctattc
    61021 tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt caattcgctt
    61081 ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct tggaggaatt
    61141 actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg aggctccagt
    61201 ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc
    61261 aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa aagttttagt
    61321 tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat taggaattaa
    61381 aactgttgga atctatgcag aagaagatga atattccgta caccgtttca aagcggatga
    61441 agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata ttgaagggat
    61501 catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg gtcttttgtc
    61561 ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg ttggacccaa
    61621 actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag ctatcgaagc
    61681 ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg atgccttgga
    61741 gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg gcggcggacg
    61801 cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag
    61861 cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc
    61921 taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc atctttttga
    61981 aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc
    62041 tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga tgaaatatgt
    62101 cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt tttactttat
    62161 cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta cagatattga
    62221 tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag aaatcggatt
    62281 gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa tcactacaga
    62341 agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc gttcacctgg
    62401 tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta
    62461 ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa cagccattca
    62521 gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa acattccatt
    62581 tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa caaccttcat
    62641 tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa acaaaacgat
    62701 gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc
    62761 attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt acgtaacggc
    62821 taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca aaggacagga
    62881 aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc tagcaactcg
    62941 tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc ttcctgaact
    63001 cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct ttttgaatga
    63061 agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat
    63121 gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg tattggtgga
    63181 atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg atagtttgaa
    63241 ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta aaatcgcaga
    63301 ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat acaatgttca
    63361 gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca ttgcaatcaa
    63421 agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg aattgaaaga
    63481 aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg gaatcatcac
    63541 ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga gtgcaatgag
    63601 tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca atggtccacg
    63661 gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg aagatgtgcg
    63721 catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag tatatatgca
    63781 tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg tcggattagg
    63841 tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt ttggcgatat
    63901 cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta tggtccaaaa
    63961 tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt
    64021 tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta aaaaattgca
    64081 aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc
    64141 agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca
    64201 agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc aaaaagcata
    64261 taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag gtatacgttt
    64321 gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc gtttagatga
    64381 aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga atggacaaag
    64441 acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc gattgaaagc
    64501 tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt tggatgtatt
    64561 agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg aagcgatgaa
    64621 aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat atgtgtcttc
    64681 aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat aataagcgaa
    64741 aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt agttattgtg
    64801 gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga aaactctgat
    64861 caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc aactggtttt
    64921 tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca agcctatgat
    64981 cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc tagtcatctc
    65041 acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact gcaaacaggg
    65101 cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac gaaaatcact
    65161 actatctata ctaatttcac ttttgagtac catgatacag aagttggaat cgaactaatg
    65221 gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt tgctatttta
    65281 ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa agatagtctt
    65341 ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca agcagataaa
    65401 tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac tttattaaat
    65461 atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt tcaaaaccac
    65521 agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc agaggaacgt
    65581 atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt ctcactgacg
    65641 aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag tgggctattt
    65701 attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga tccttacaac
    65761 catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc taaagaaaat
    65821 atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga tcgttaacga
    65881 agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa
    65941 aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa aagtttgcca
    66001 gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg catatggtga
    66061 gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga
    66121 tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa gtctgttgga
    66181 tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag
    66241 cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg caattagaca
    66301 atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac agcttaaaac
    66361 aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg
    66421 tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc cgtaaattac
    66481 attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat tttgccgaaa
    66541 aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag ctaaatacag
    66601 aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa ataaaaaata
    66661 acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat actagtacca
    66721 gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg cgggcgccgt
    66781 ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa agaatccata
    66841 ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt tgcaggaagc
    66901 ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg cgtagacaaa
    66961 aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga accagagaaa
    67021 tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa
    67081 caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc
    67141 cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg cgaaacggat
    67201 aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa acagatttat
    67261 ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt gcattattcc
    67321 caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt gcggctaaat
    67381 tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc
    67441 cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat gtaaaagtca
    67501 tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca gatgcgttga
    67561 tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct cagatgaatc
    67621 atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa tatagtcacg
    67681 tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc agctacttac
    67741 cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg
    67801 ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt agtagttccc
    67861 ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact aattacggtc
    67921 aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg tatcaggcaa
    67981 gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa
    68041 gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca gtattatatg
    68101 gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa
    68161 atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt ctttggcaag
    68221 ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag tacagaagca
    68281 tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat
    68341 aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga taaaaaggca
    68401 ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga tgttcgtatt
    68461 aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga aatctatgaa
    68521 caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg aacgatcaac
    68581 agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag tgcggataaa
    68641 gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt taagaaagaa
    68701 tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc
    68761 tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct agagaaaatg
    68821 aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa cccctctttt
    68881 tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt aattagatgt
    68941 tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga
    69001 atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa
    69061 tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc ttttgtatcg
    69121 caagtggttt tttattatca aatttgctat tttatagaga aacagaagag actgctgcaa
    69181 aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa ggaaacgatg
    69241 tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt gtgaaggagc
    69301 ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag cgtgcaggtg
    69361 gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata gaagatcagc
    69421 aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat cagttggcat
    69481 cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat atcaatacag
    69541 ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg caagaaatca
    69601 ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag gagatttctg
    69661 gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt
    69721 caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat aatccaattc
    69781 taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt gattgttcgc
    69841 aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat atctgggatt
    69901 catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga acgctttgcc
    69961 aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac gaatgatcta
    70021 gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg atgatagttc
    70081 agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca gcagtcgatg
    70141 agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct gttctcacag
    70201 aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc ttgaacaaat
    70261 ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg gcagtgaagg
    70321 gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt gtttaagcaa
    70381 tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt
    70441 caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat gaacgcattc
    70501 cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt acgtgtacac
    70561 gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc ggctataatg
    70621 gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg gatggacact
    70681 gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc
    70741 cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt acaaaggctt
    70801 tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat gatccttatg
    70861 cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta gactcacagc
    70921 atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt agaaaggcat
    70981 gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt gttgtattac
    71041 actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt ttcgctaaaa
    71101 gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc catttcttat
    71161 aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat tactgtattt
    71221 acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt atcagagaat
    71281 aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa aacatggctt
    71341 aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc tccagcaaaa
    71401 aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag aatttctgga
    71461 cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg aaactggctt
    71521 cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca aaaaacaagt
    71581 gtatatatta attctttatt atggggctat aaagatgcag gattcagaga accagaagaa
    71641 atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca catccaattt
    71701 tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca cgtcagtatt
    71761 attccgttga gtctactaac tatctgttat gtttctattg caggaagttc tatttctgtt
    71821 ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa taagcattat
    71881 tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga tcctcgattt
    71941 ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat cctgatttct
    72001 tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat gatcacgatg
    72061 ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt tacattactg
    72121 attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac actcttcaat
    72181 cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt atttgagcga
    72241 ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct tgtgctatct
    72301 gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca acctgtgaaa
    72361 cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt tttacgcttt
    72421 tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt gcaaagtcct
    72481 tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga aagagaaaag
    72541 tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata tctaaaaggt
    72601 caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga tcatgtggga
    72661 gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg ttggggcgct
    72721 ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg tactgagatc
    72781 attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt attgactcct
    72841 tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga atataacaat
    72901 aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt gcttttgatt
    72961 tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt
    73021 aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg cggcgtaaat
    73081 aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa tcagataaaa
    73141 acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt ttttcatcct
    73201 catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg taggataatt
    73261 tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac gtaaagaaaa
    73321 acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc aagttcggac
    73381 agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt taagttttga
    73441 catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc cgtttttcgg
    73501 agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa aagtaaacaa
    73561 tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat tagaatcgac
    73621 tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag taacgaaaca
    73681 attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca
    73741 atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg caattgattt
    73801 attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac agaaattggt
    73861 cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat tagttcctaa
    73921 aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga agacggaaca
    73981 agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca aaattaatgc
    74041 gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa agattggtta
    74101 tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa aacttgccat
    74161 gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc aattagttga
    74221 gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc aactattcgt
    74281 tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc aaagcatgcg
    74341 ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg aagccgattc
    74401 ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg
    74461 attagacctt ttgattcagc catatcaata gctttaacag cttctttata taacgcatct
    74521 acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt acgcatagcg
    74581 cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg tttgattgca
    74641 gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc gtgttaccgc
    74701 ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg aacatgacaa
    74761 gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg atagtacaag
    74821 ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca aactagattt
    74881 ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt attatataac
    74941 aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt tgttctttgt
    75001 ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag taaaggctat
    75061 tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat tttttttatg
    75121 accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt atttaggggc
    75181 aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat cacacggtcc
    75241 tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac gtacgtatca
    75301 attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag taacctgaaa
    75361 acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat tctttctttt
    75421 gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc
    75481 gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt gattaaaaaa
    75541 agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca aaaagcagta
    75601 tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag aatcaaaagt
    75661 accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc gaacattgta
    75721 tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa tctcctaaaa
    75781 tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt aagtgatacc
    75841 agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta aaatcgtgcc
    75901 gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac ccttggaaga
    75961 taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg atttaaaggc
    76021 ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa tagccgctgt
    76081 gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa aggaacggga
    76141 ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac tttgattttt
    76201 agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca tgaaatatat
    76261 tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa gtaggaattc
    76321 atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat cataaaaaaa
    76381 cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga cttacatttt
    76441 tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt cagaataacc
    76501 aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa ttgcaggaag
    76561 ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg tttgatgatc
    76621 cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc gcttttcatt
    76681 aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc gacacctaaa
    76741 gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc atttacttgg
    76801 taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca gcttgcatag
    76861 taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt
    76921 atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg ggtatttgct
    76981 tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga aagataaatc
    77041 acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt gctgattatc
    77101 atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa ataggtagtt
    77161 tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc aggtattgtg
    77221 caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg ttccatagca
    77281 acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt tacaactggt
    77341 tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc agaaagcttt
    77401 tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga aagagcagct
    77461 agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac taataatacc
    77521 attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg gagaaggatc
    77581 attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg ttcgataaat
    77641 gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat cttagcaaag
    77701 acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg attcagcgtt
    77761 cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc gcttaatgca
    77821 gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac agctgctcct
    77881 ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc ttgcatttgt
    77941 ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc agtaatttct
    78001 ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc
    78061 aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc taaagccatc
    78121 aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc cgatgtttgt
    78181 ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt aagagagaat
    78241 cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc tgcaaaagct
    78301 tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag cataataaaa
    78361 atgtaattga taataataac agctaaatga gaacgataat ggttatcatt tggtgattaa
    78421 gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa aaaccagaac
    78481 atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc aataaccaat
    78541 caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa
    78601 atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga atgttcctac
    78661 aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat atttgacgat
    78721 cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa actctgtagc
    78781 gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag aacgggatgt
    78841 tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt ggtacgtaaa
    78901 gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga caataataaa
    78961 ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga aatttgcttc
    79021 taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa cgatcacttt
    79081 aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag aaaatgggtt
    79141 taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga taacgacatt
    79201 gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg ctaggatcaa
    79261 atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac cgagaatcac
    79321 tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag atacatctta
    79381 tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga aaaacttgag
    79441 aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt tcagactttg
    79501 ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga gtcgcgacta
    79561 tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga aaatcgaccg
    79621 tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac aggctgttta
    79681 ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac taaaacattt
    79741 ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca tacacggtct
    79801 cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac agcattagca
    79861 atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa attcaataca
    79921 cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc gaaacaccat
    79981 tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg ttctcctaga
    80041 ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg
    80101 acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt tgtgaggggt
    80161 gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg
    80221 atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac gattgaaaaa
    80281 aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc
    80341 aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt gttctaaatt
    80401 ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc cagaaacgac
    80461 aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag ggacgacgcg
    80521 aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg caggcgagac
    80581 aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa tcggatcacc
    80641 tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg cgcgtaaagg
    80701 ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg
    80761 gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt caatgatcaa
    80821 aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg tttttaatct
    80881 ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca ttagtataac
    80941 gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc tgaattatca
    81001 gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt catagcattt
    81061 taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc ctatatccct
    81121 gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc taaactaggt
    81181 tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt caaaaatatc
    81241 tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag
    81301 taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga
    81361 aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat attacctgat
    81421 cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata tagatgaaat
    81481 caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac ttttctttca
    81541 tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag aaataaatct
    81601 gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg aacatcagac
    81661 aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag aatggcttgt
    81721 ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga aattccccca
    81781 tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa cgatccgtac
    81841 gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct ctacgttatt
    81901 ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc taacggctaa
    81961 aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata caggagctga
    82021 agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg agacgacctg
    82081 taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg aaggtttatg
    82141 ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa
    82201 gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg acgtaatcat
    82261 tggggattac aattatttat ttgatccaac tgtttattta agacgttttt ttgaagatga
    82321 aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga atcgttcccg
    82381 agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg aagctatacc
    82441 aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat ttgattcgat
    82501 ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac cggctgaatc
    82561 tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg cagaatttcc
    82621 tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc attttttgaa
    82681 ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt atcgcgatct
    82741 gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt tggataaagg
    82801 aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc aggaaacact
    82861 ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag gaaaccagca
    82921 agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca gtctggcaaa
    82981 attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt tcgttttctt
    83041 tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat atcccgacag
    83101 caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa
    83161 atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg ggattttttc
    83221 agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag gtgtaggttt
    83281 gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga aggaacacct
    83341 tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg
    83401 agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac gattttctag
    83461 tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt
    83521 tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag taaatcaaaa
    83581 aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag tgaattgcaa
    83641 ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat catcacggtt
    83701 cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca gtttttcatt
    83761 tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc
    83821 agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga tatttttgat
    83881 tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt cgattttgaa
    83941 atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta ctgtagaatc
    84001 cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat tttttacttc
    84061 ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag gaaccctcct
    84121 gatatacata taaatattta ccatacatct atcttatcat aggattaggg cagattctta
    84181 cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat agataaactg
    84241 ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact
    84301 gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac cggattgacc
    84361 gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc agggatgaat
    84421 gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa gatagacaca
    84481 ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct caaagtagga
    84541 aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg
    84601 cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg cggtgtttta
    84661 ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg gatggatatc
    84721 atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat gctgattcta
    84781 aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc cttgtatacg
    84841 attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag tcatcaaaaa
    84901 gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg
    84961 atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa acaagggaac
    85021 atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa
    85081 atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg ccggtttgca
    85141 aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt
    85201 tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat tacatctaat
    85261 aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt tacaagcagc
    85321 taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca
    85381 aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct tgctgccttg
    85441 gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt gatttatgat
    85501 atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac aactatgggc
    85561 tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga agtgccagaa
    85621 gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga taagatgagc
    85681 cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag ctatgagaag
    85741 caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat aaaaggtttc
    85801 tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt gttctattga
    85861 taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca gtttgatttt
    85921 cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt gttgtgcttt
    85981 aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc cgattttttt
    86041 tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac gataattagg
    86101 ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca gtttatggcg
    86161 caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga cggttgcggg
    86221 aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt ttacataatc
    86281 taaaaattca gcaaatgtat agccataagc attgttgata aatccactct catttggtcc
    86341 aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt tgatcagttt
    86401 catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag cactattgtt
    86461 gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat catcgaacaa
    86521 tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt acttatgatc
    86581 tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg atagtaatca
    86641 acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa
    86701 aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt ttaacattga
    86761 aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga tggagttgct
    86821 cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa aaagaacaaa
    86881 aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat gcggctatgg
    86941 cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag
    87001 tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga gcgatgagaa
    87061 agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg aaccagattc
    87121 aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat agattgattc
    87181 aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt taaggaataa
    87241 agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg acctcttact
    87301 atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg tttgcttccc
    87361 agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt tctgctactc
    87421 tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat tggtaacggc
    87481 cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt gcgatccatt
    87541 cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca ggagcaggtt
    87601 gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt
    87661 gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct tgaggttcag
    87721 cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DAZKPO010000003        69664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000003 DAZKPO010000000
VERSION     DAZKPO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.003"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(606..962)
                     /locus_tag="ACG8NZ_000173"
     CDS             complement(606..962)
                     /locus_tag="ACG8NZ_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0537882.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="ACG8NZ_000174"
     CDS             complement(976..1386)
                     /locus_tag="ACG8NZ_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0537883.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="ACG8NZ_000175"
     CDS             complement(1732..3918)
                     /locus_tag="ACG8NZ_000175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0537884.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="ACG8NZ_000176"
     CDS             4136..4819
                     /locus_tag="ACG8NZ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0537885.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="ACG8NZ_000177"
     CDS             complement(4900..5619)
                     /locus_tag="ACG8NZ_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0537886.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="ACG8NZ_000178"
     CDS             complement(5931..6137)
                     /locus_tag="ACG8NZ_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537887.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="ACG8NZ_000179"
     CDS             6382..6984
                     /locus_tag="ACG8NZ_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0537888.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8405
                     /locus_tag="ACG8NZ_000180"
     CDS             7125..8405
                     /locus_tag="ACG8NZ_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0537889.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8418..9146
                     /locus_tag="ACG8NZ_000181"
     CDS             8418..9146
                     /locus_tag="ACG8NZ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0537890.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9215..10738)
                     /locus_tag="ACG8NZ_000182"
     CDS             complement(9215..10738)
                     /locus_tag="ACG8NZ_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0537891.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10990..12171
                     /locus_tag="ACG8NZ_000183"
     CDS             10990..12171
                     /locus_tag="ACG8NZ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0537892.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12231..13859)
                     /gene="treC"
                     /locus_tag="ACG8NZ_000184"
     CDS             complement(12231..13859)
                     /gene="treC"
                     /locus_tag="ACG8NZ_000184"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0537893.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14310..17021)
                     /locus_tag="ACG8NZ_000185"
     CDS             complement(14310..17021)
                     /locus_tag="ACG8NZ_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0537894.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17031..17732)
                     /locus_tag="ACG8NZ_000186"
     CDS             complement(17031..17732)
                     /locus_tag="ACG8NZ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0537895.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17918..18517
                     /locus_tag="ACG8NZ_000187"
     CDS             17918..18517
                     /locus_tag="ACG8NZ_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0537896.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18598..19611
                     /locus_tag="ACG8NZ_000188"
     CDS             18598..19611
                     /locus_tag="ACG8NZ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0537897.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19719..20636
                     /gene="rbsK"
                     /locus_tag="ACG8NZ_000189"
     CDS             19719..20636
                     /gene="rbsK"
                     /locus_tag="ACG8NZ_000189"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0537898.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20664..21059
                     /gene="rbsD"
                     /locus_tag="ACG8NZ_000190"
     CDS             20664..21059
                     /gene="rbsD"
                     /locus_tag="ACG8NZ_000190"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0537899.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21059..22432
                     /locus_tag="ACG8NZ_000191"
     CDS             21059..22432
                     /locus_tag="ACG8NZ_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0537900.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22789..24933
                     /locus_tag="ACG8NZ_000192"
     CDS             22789..24933
                     /locus_tag="ACG8NZ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0537901.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24985..26025)
                     /locus_tag="ACG8NZ_000193"
     CDS             complement(24985..26025)
                     /locus_tag="ACG8NZ_000193"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0537902.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26302..28104)
                     /locus_tag="ACG8NZ_000194"
     CDS             complement(26302..28104)
                     /locus_tag="ACG8NZ_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0537903.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28118..28870)
                     /locus_tag="ACG8NZ_000195"
     CDS             complement(28118..28870)
                     /locus_tag="ACG8NZ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0537904.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28879..29241)
                     /locus_tag="ACG8NZ_000196"
     CDS             complement(28879..29241)
                     /locus_tag="ACG8NZ_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0537905.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29417..30289)
                     /locus_tag="ACG8NZ_000197"
     CDS             complement(29417..30289)
                     /locus_tag="ACG8NZ_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0537906.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30340..30828)
                     /locus_tag="ACG8NZ_000198"
     CDS             complement(30340..30828)
                     /locus_tag="ACG8NZ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0537907.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30966..31757)
                     /locus_tag="ACG8NZ_000199"
     CDS             complement(30966..31757)
                     /locus_tag="ACG8NZ_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0537908.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31812..32420)
                     /locus_tag="ACG8NZ_000200"
     CDS             complement(31812..32420)
                     /locus_tag="ACG8NZ_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0537909.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32413..32730)
                     /locus_tag="ACG8NZ_000201"
     CDS             complement(32413..32730)
                     /locus_tag="ACG8NZ_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0537910.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32886..33614)
                     /locus_tag="ACG8NZ_000202"
     CDS             complement(32886..33614)
                     /locus_tag="ACG8NZ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0537911.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33773..34654
                     /locus_tag="ACG8NZ_000203"
     CDS             33773..34654
                     /locus_tag="ACG8NZ_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0537912.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34891..35934)
                     /locus_tag="ACG8NZ_000204"
     CDS             complement(34891..35934)
                     /locus_tag="ACG8NZ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0537913.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35937..36605)
                     /locus_tag="ACG8NZ_000205"
     CDS             complement(35937..36605)
                     /locus_tag="ACG8NZ_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0537914.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36747..39083
                     /locus_tag="ACG8NZ_000206"
     CDS             36747..39083
                     /locus_tag="ACG8NZ_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0537915.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39198..39509)
                     /locus_tag="ACG8NZ_000207"
     CDS             complement(39198..39509)
                     /locus_tag="ACG8NZ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537916.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39690..41165
                     /locus_tag="ACG8NZ_000208"
     CDS             39690..41165
                     /locus_tag="ACG8NZ_000208"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0537917.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41212..41796
                     /locus_tag="ACG8NZ_000209"
     CDS             41212..41796
                     /locus_tag="ACG8NZ_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0537918.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41798..42217
                     /locus_tag="ACG8NZ_000210"
     CDS             41798..42217
                     /locus_tag="ACG8NZ_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0537919.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42498..42782)
                     /locus_tag="ACG8NZ_000211"
     CDS             complement(42498..42782)
                     /locus_tag="ACG8NZ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0537920.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43134..43844)
                     /locus_tag="ACG8NZ_000212"
     CDS             complement(43134..43844)
                     /locus_tag="ACG8NZ_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0537921.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43841..45313)
                     /locus_tag="ACG8NZ_000213"
     CDS             complement(43841..45313)
                     /locus_tag="ACG8NZ_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0537922.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45451..45924
                     /locus_tag="ACG8NZ_000214"
     CDS             45451..45924
                     /locus_tag="ACG8NZ_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0537923.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46077..46628)
                     /locus_tag="ACG8NZ_000215"
     CDS             complement(46077..46628)
                     /locus_tag="ACG8NZ_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0537924.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46733..47263
                     /locus_tag="ACG8NZ_000216"
     CDS             46733..47263
                     /locus_tag="ACG8NZ_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0537925.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47746..48189)
                     /locus_tag="ACG8NZ_000217"
     CDS             complement(47746..48189)
                     /locus_tag="ACG8NZ_000217"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0537926.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48205..49365)
                     /locus_tag="ACG8NZ_000218"
     CDS             complement(48205..49365)
                     /locus_tag="ACG8NZ_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0537927.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49872..51572
                     /locus_tag="ACG8NZ_000219"
     CDS             49872..51572
                     /locus_tag="ACG8NZ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537928.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51674..52507)
                     /locus_tag="ACG8NZ_000220"
     CDS             complement(51674..52507)
                     /locus_tag="ACG8NZ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0537929.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52811..54976)
                     /locus_tag="ACG8NZ_000221"
     CDS             complement(52811..54976)
                     /locus_tag="ACG8NZ_000221"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0537930.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55236..57530
                     /locus_tag="ACG8NZ_000222"
     CDS             55236..57530
                     /locus_tag="ACG8NZ_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0537931.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57523..58188
                     /gene="pgmB"
                     /locus_tag="ACG8NZ_000223"
     CDS             57523..58188
                     /gene="pgmB"
                     /locus_tag="ACG8NZ_000223"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0537932.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58181..59188
                     /locus_tag="ACG8NZ_000224"
     CDS             58181..59188
                     /locus_tag="ACG8NZ_000224"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0537933.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59210..60229
                     /locus_tag="ACG8NZ_000225"
     CDS             59210..60229
                     /locus_tag="ACG8NZ_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0537934.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="ACG8NZ_000226"
     CDS             complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="ACG8NZ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0537935.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61986..63407)
                     /gene="tyrP"
                     /locus_tag="ACG8NZ_000227"
     CDS             complement(61986..63407)
                     /gene="tyrP"
                     /locus_tag="ACG8NZ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0537936.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="ACG8NZ_000228"
     CDS             complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="ACG8NZ_000228"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0537937.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65818..67074)
                     /gene="tyrS"
                     /locus_tag="ACG8NZ_000229"
     CDS             complement(65818..67074)
                     /gene="tyrS"
                     /locus_tag="ACG8NZ_000229"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0537938.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67186..67391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67654..68955
                     /locus_tag="ACG8NZ_000230"
     CDS             67654..68955
                     /locus_tag="ACG8NZ_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0537939.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69024..69623)
                     /locus_tag="ACG8NZ_000231"
     CDS             complement(69024..69623)
                     /locus_tag="ACG8NZ_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0537940.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20905 a        14093 c        12342 g        22324 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7381 ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct tcggttattg
     7441 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7501 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7561 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7621 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7681 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7741 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7801 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7861 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7921 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7981 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     8041 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     8101 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     8161 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     8221 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     8281 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8341 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8401 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8461 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8521 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8581 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8641 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8701 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8761 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8821 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8881 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8941 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     9001 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     9061 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     9121 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     9181 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     9241 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9301 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9361 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9421 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9481 gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct ttctcactga
     9541 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9601 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9661 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9721 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9781 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9841 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9901 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9961 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
    10021 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
    10081 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
    10141 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
    10201 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
    10261 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10321 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10381 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10441 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10501 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10561 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10621 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10681 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10741 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10801 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10861 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10921 aacctctatc attttccttt tatacccgct tttgtctata atgaatttat agataaagga
    10981 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    11041 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    11101 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    11161 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    11221 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    11281 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11341 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11401 tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc tgatatagct
    11461 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11521 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11581 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11641 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11701 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11761 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11821 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11881 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11941 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    12001 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    12061 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    12121 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    12181 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    12241 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12301 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12361 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12421 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12481 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12541 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12601 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12661 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12721 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12781 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12841 gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg attgagcgca
    12901 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatcttt
    12961 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    13021 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    13081 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    13141 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    13201 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    13261 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13321 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13381 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13441 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13501 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13561 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13621 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13681 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13741 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13801 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13861 gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata caagttatca
    13921 tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13981 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    14041 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    14101 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    14161 gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc cttcggtatt
    14221 agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct aatactcagg
    14281 tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc atcaatcata
    14341 tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa tacgatagtt
    14401 aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat gacattctcc
    14461 atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa tcctccgatg
    14521 attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac ttctttatca
    14581 aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat attgatatta
    14641 gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa gattaagaca
    14701 accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga catgaaagac
    14761 gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc ttcttttgtt
    14821 gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc agtaactgaa
    14881 gcataatatt caggggacat ataaataaag ctacctagat agtttttcac gatttctttt
    14941 acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc tcctgtagat
    15001 aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg caaggagcgt
    15061 gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt tttacttggt
    15121 acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac ggtttgagta
    15181 tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc tgctttttga
    15241 cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat gatcggtcca
    15301 aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc aaccatcagc
    15361 ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata tcggaagaga
    15421 tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt gatgtattca
    15481 agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc aggccgttct
    15541 cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa gaatgcggca
    15601 gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat atcataccgc
    15661 tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat cgttcctaaa
    15721 acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc gatttcaact
    15781 ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc taccatacgt
    15841 gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg aaaaacattg
    15901 gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac attttctgaa
    15961 cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc atttcgattc
    16021 aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct ttcttgttca
    16081 gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg cgcttcagga
    16141 agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc taaccgtgtt
    16201 cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg ttctaattct
    16261 tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg taaatacttt
    16321 tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta cgtattcaaa
    16381 ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt aaaaacggat
    16441 tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta tcctctctcc
    16501 aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc tttttctttc
    16561 aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc ttccgctttt
    16621 tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa ttgtagcttg
    16681 ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt gccagtattg
    16741 gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc tgcttgagca
    16801 atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag tcctgctgta
    16861 ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac acctacaaat
    16921 aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt agattgccga
    16981 atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc ctaccataca
    17041 atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt agcatcatta
    17101 atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat gatcaaaacg
    17161 gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt gcccgtttca
    17221 aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt agctagtgca
    17281 cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa gttattcaat
    17341 cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc aggagaaact
    17401 tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt gtagaattgg
    17461 aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga aaattgcgca
    17521 atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc acctaaaata
    17581 ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa ttcgcctttt
    17641 tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat tttgtatcgt
    17701 ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc acgatatttc
    17761 tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc actgaatttc
    17821 tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt atgatttact
    17881 tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat
    17941 gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag agatcgtaaa
    18001 taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg atttgttagt
    18061 aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt
    18121 cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt
    18181 cactgattta cttttgaagg attctttgaa agatacagat aatatgttga ctatcacaaa
    18241 atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat
    18301 cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga
    18361 tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg atcgtgacaa
    18421 agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac
    18481 gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat
    18541 ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg
    18601 gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact
    18661 gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt
    18721 ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc
    18781 aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca
    18841 ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat
    18901 gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg
    18961 gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa
    19021 aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc
    19081 gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctagga
    19141 catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca cgctcggatt
    19201 gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact gatcctctta
    19261 cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct tgattctgct
    19321 gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg cggattagag
    19381 gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa tatagatatg
    19441 tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga attgggacaa
    19501 acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg ggaagaaaaa
    19561 agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata gctttcaagg
    19621 tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa aaaataattt
    19681 tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt acagtaattg
    19741 gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca ggcgaaacga
    19801 tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa gcagtggcag
    19861 ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac agcgaaggac
    19921 aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata gctgaaatcc
    19981 aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa aatagtatct
    20041 tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa aacaagcaac
    20101 tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat gcgacgatcc
    20161 tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cctgaatcct gcaccagctc
    20221 gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca aatgaaacag
    20281 aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc gcagcagccg
    20341 aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt gccggcgctt
    20401 tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat gccgttgaca
    20461 caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag ccagacctta
    20521 gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct gtgcagtctt
    20581 atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca gtctaaaatt
    20641 aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca gatatttcac
    20701 gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca ggcatgcctg
    20761 tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca agttttttat
    20821 ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg gcggaagaaa
    20881 taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca gatacaccga
    20941 ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat gcatttattc
    21001 gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg gtcttctaat
    21061 ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata tgctgattga
    21121 acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc ggcttcgtat
    21181 caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa tcccttccat
    21241 taagtatttc attttactcg atagatatgc agaaataatc atggatcagg gattggctcc
    21301 tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa agctaaatcc
    21361 gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc cgctgtttgc
    21421 cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct ggcttagtga
    21481 aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt attttctgcc
    21541 aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata ttgcggctgt
    21601 cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca gtggcaaagc
    21661 agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta ctgtcatgcc
    21721 ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa aatatattcg
    21781 cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg ttcctttgac
    21841 gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg ccgcaattac
    21901 ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg gttggaatat
    21961 tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta atatctctgc
    22021 ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa tggctggtgc
    22081 agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct ccggttctgc
    22141 ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa ttgcgatccc
    22201 attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag ctgtaatggg
    22261 atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat tgcctgcatt
    22321 catttccggt acttttttct ggtacttagc aggactggct gttagtctag tcgtagcaat
    22381 gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat agttaatgaa
    22441 aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt aggatcatta
    22501 tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat gcataaccat
    22561 ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa tgaacgtcaa
    22621 agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga atacacagac
    22681 agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa tgtctaatga
    22741 atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat gaaaaataaa
    22801 aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt gtcgaccaat
    22861 accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga tacgactaca
    22921 tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac tggaacaact
    22981 aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc atcagaaaat
    23041 gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg taagatccaa
    23101 ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac ggaaagtgga
    23161 acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc agctgaaggt
    23221 gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga tgcaaagacc
    23281 tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg accggatact
    23341 tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga tccggaaacg
    23401 ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa ttcaaacaat
    23461 aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc tggatcgaat
    23521 aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc gaatggcgga
    23581 acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga gaatccatct
    23641 tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc tacaaataca
    23701 acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc atcttggaaa accatatgta
    23761 tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta cgtatatatg
    23821 aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc aggtacgcag
    23881 attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc ttccggtgtt
    23941 actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc tcaaccagga
    24001 gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt ccgagtaaac
    24061 ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat tttagatgga
    24121 ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt
    24181 gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct
    24241 caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac
    24301 ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac acttgaacaa
    24361 gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag
    24421 gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc
    24481 tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa
    24541 ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac
    24601 gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgct
    24661 gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg
    24721 ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc
    24781 cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat accagaaaca
    24841 actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa
    24901 tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact
    24961 gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa
    25021 ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc
    25081 tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat
    25141 ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt
    25201 tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta gtaaaccaga
    25261 ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt ggcgaaaatc
    25321 actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac
    25381 tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt
    25441 caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac
    25501 atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca
    25561 tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt
    25621 ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac cattgatgac
    25681 tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt
    25741 gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtaat
    25801 ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt
    25861 tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg
    25921 aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatcgt
    25981 aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc
    26041 ttttctttca tttgcactga ttccagtata catggaaacc gttttcttaa cagcttattc
    26101 cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa aaaggttgtg
    26161 acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc ttgcatctgc
    26221 ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc tgttcttatt
    26281 acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag tatataacca
    26341 tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg gaacccaaat
    26401 atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata agacatggac
    26461 tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga tcgattgttt
    26521 aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa tgtcactctt
    26581 gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga aagctaaacc
    26641 taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt catacatttc
    26701 atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt tattttgagc
    26761 aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa ctagctgtaa
    26821 agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct tgatttcttt
    26881 ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca gttcattcat
    26941 ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt catgttttat
    27001 catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc ccgcatcttc
    27061 tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt gctgatcttc
    27121 tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca agtctgcaat
    27181 ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa acagcatagc
    27241 gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg acaatgtgaa
    27301 attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata aaaagaaaat
    27361 gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg ctacccctat
    27421 accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc ctagaaaagc
    27481 aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc ttgttggtgt
    27541 ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag cattgacaac
    27601 agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc cgtgaatact
    27661 aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc ggtggacgat
    27721 acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc ctactaagaa
    27781 tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca tcataaacat
    27841 tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa tataaacgaa
    27901 tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag ccgcaacgat
    27961 cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt aaaaaatagc
    28021 agatgccata actaatcctg agaataacac caagtagtct cttaaacggc tcttgatccc
    28081 agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag tttccaagaa
    28141 tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc acttcttgat
    28201 ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa aacggatcat
    28261 gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc gtatctaaaa
    28321 gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat agaatcgttg
    28381 gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg gaaatctcag
    28441 aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt tggacacgtt
    28501 gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata ttttcttgtg
    28561 ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca ttcgcacgaa
    28621 aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt cggatatgcc
    28681 catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta ccagaaccag
    28741 aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg ccgcttaatg
    28801 cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt acttcgacta
    28861 ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt tcggcacgtc
    28921 ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg tgatttttaa
    28981 atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt ctttttcctt
    29041 accctctttc gtatagccag gcaggatata tctatagttg actgtttctt gtccagagtc
    29101 tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt cttctccacc
    29161 cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga aaacaagtag
    29221 accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac tgattataga
    29281 aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt ttgtcatttt
    29341 ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc cttcttccta
    29401 tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata acaggttgcg
    29461 ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact ttccgatcat
    29521 cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga gtgattgtga
    29581 cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt tgacgttgga
    29641 gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt tcgattactt
    29701 cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca gacactgccg
    29761 tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg tattggctag
    29821 cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa tacgctacat
    29881 cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag atttcgatgg
    29941 ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga actttcaacg
    30001 aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct cgaatcgtac
    30061 ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact acagttgccg
    30121 aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt gcccaaactt
    30181 tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat acgaccaatc
    30241 cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg ctacttcctg
    30301 cgctccttta tccttcaata aatatctaat gttacttttt taattttttt ttcgaatacg
    30361 cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac cgcttgcctg
    30421 tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt gcttttcttc
    30481 tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag gcaaatcttc
    30541 ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata ttcctgtatc
    30601 gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat gtttgaaaac
    30661 ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat taaccatcac
    30721 tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag tatgtaatct
    30781 ttccatttct gtttttaact tttctctttg accttcttct accttcatat actctttcac
    30841 cctgttttct ttttagatat tttctttctt attttactat agttatgtgc gttaagcgat
    30901 gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc cagttgagta
    30961 caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga tgtgtcttta
    31021 gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat tggctggtag
    31081 tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt ttccatctca
    31141 tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat caaactgtct
    31201 tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt ggcctcatct
    31261 aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa ttcattgaac
    31321 gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc gttgatatca
    31381 attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc agggacaaca
    31441 atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga ttttttgact
    31501 gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt cttatcataa
    31561 gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt ttctgcatgg
    31621 atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc gatcaaccga
    31681 ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc acctgctagg
    31741 aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa tctcaattta
    31801 tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa acgtcgtccc
    31861 atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt cagcaacagt
    31921 ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa gaataatcca
    31981 ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag gccaacacct
    32041 aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc aaccaatagt
    32101 cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga agcaaataaa
    32161 ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac taactcgttt
    32221 tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa ctgctttggt
    32281 gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa atattcctca
    32341 taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg atccacaact
    32401 tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag aatctcgaaa
    32461 aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg tcaacgagta
    32521 atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc cttctttttt
    32581 caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt cttgtacgat
    32641 ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca cagtaccatc
    32701 aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca agtgtaatat
    32761 tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt tgtcaaatac
    32821 aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct tctctttgat
    32881 ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg
    32941 atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg
    33001 gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc
    33061 aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt
    33121 gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca
    33181 aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc taaacttcca
    33241 tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt gctgataaat
    33301 tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc
    33361 agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat cgctgttcct
    33421 atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc
    33481 atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat
    33541 ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac attgatcaaa
    33601 actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa atcagaaaaa
    33661 tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa gattacacca
    33721 attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata tgatggatat
    33781 ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca aaactgctga
    33841 tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag aaacagaatt
    33901 agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag ctggaagctt
    33961 attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag aagtgcttga
    34021 aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga caatgatcaa
    34081 ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat ttacagtcca
    34141 gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac atagtgacac
    34201 gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt tgcctactga
    34261 aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta aaaagaaatt
    34321 ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta cgaacttgta
    34381 tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct cttataaagg
    34441 agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg ctattgttat
    34501 ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta tttctccaac
    34561 aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg cttcagatac
    34621 attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt ccatctatcc
    34681 acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat gtccttgttt
    34741 caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt tatgccaaaa
    34801 gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta aaaaccttca
    34861 atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta tcattgctgt
    34921 cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt ctagaatttt
    34981 tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat ctccttgata
    35041 aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg aatcggtcac
    35101 ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc cattgatttg
    35161 attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa actcttcatt
    35221 gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt tctcccattt
    35281 aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact ctttcttttc
    35341 cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg ttaattgtga
    35401 aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt cgtcagagag
    35461 atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg ttttgaattc
    35521 atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga gttcttggac
    35581 cataacgttg aaatctttat acagttgctg tacttctgct actttctgct gttcatccat
    35641 tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg cactgattgg
    35701 atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca tactgaaccc
    35761 cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag gcgagataga
    35821 atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta agatcgaaat
    35881 gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt tcatctttaa
    35941 ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc cgtcttttca
    36001 aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt attcgtatct
    36061 aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg ataagacagt
    36121 aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg atcttgtaac
    36181 actgtataag catctagctc taattttgtt tgtccgatta tcaagctact gctgtaattg
    36241 atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac atcgatgggt
    36301 tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc aaaatcggcc
    36361 tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga gtctagcaag
    36421 tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat acgtgtcgct
    36481 tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa gcccactcgt
    36541 tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac taaaatacga
    36601 ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta gttcatcttc
    36661 agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta gtataccgaa
    36721 tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa aaatattata
    36781 aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc agacaaaaag
    36841 aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg aatgtgattg
    36901 gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct actaaagact
    36961 ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt tttcaaagtt
    37021 acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg acattaagtg
    37081 gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga gtcggcttga
    37141 caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag cgagtggcca
    37201 tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca accggagcgt
    37261 tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg aaagaaaaat
    37321 tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat cgaatcattg
    37381 aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacga ccaaaagatg
    37441 atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag ctttccttca
    37501 acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc agtattggga
    37561 tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa atcgataaca
    37621 cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg actaaccaga
    37681 ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc aaaacgatta
    37741 ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat caaacatacg
    37801 tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt acccgtacta
    37861 caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc ttctcgaatc
    37921 aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg acaggtgtgg
    37981 gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta aaagacaatt
    38041 attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg atcgtagacg
    38101 gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg aaagaagacg
    38161 aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac aacaatactt
    38221 actatacaaa acttccaact gggaatttta tccctaatac agattatgat gcgatgtatc
    38281 agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct tcttctacaa
    38341 tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa atcgtcaacg
    38401 aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat gtcttaacta
    38461 ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt ggagacagct
    38521 tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag atcttggatt
    38581 atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat actgatttag
    38641 ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat gtccttattg
    38701 cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt acttatacat
    38761 ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca cgtaaaaaag
    38821 acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct gggatattag
    38881 gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac aacatgacgg
    38941 atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg atcgtctcta
    39001 ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa aaagacgccg
    39061 ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga gtcataagag
    39121 cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg tttcgcgctg
    39181 ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta ccacaactct
    39241 tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc aatcgttact
    39301 tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt tcctcttcct
    39361 ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt cacgtatttt
    39421 acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac ttttactgct
    39481 ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag tatgcgcttt
    39541 cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat ccattccatt
    39601 gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt tactatagat
    39661 aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat ttaagaaaag
    39721 tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt gaaatcgatc
    39781 ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca gtaaaacgtc
    39841 cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc ggcacacgtg
    39901 tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac catgattatc
    39961 gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct gtgaaagtag
    40021 acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca gacttcgata
    40081 ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca tacatcacaa
    40141 tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg atccatcgta
    40201 tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga cgccacattg
    40261 gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact atcaatattg
    40321 gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact ccacttggct
    40381 acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc gttgatggta
    40441 tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca gaaatcatgc
    40501 caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg ccagaatttc
    40561 ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa gcagttactc
    40621 accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa cacgtatcta
    40681 tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct attccaggaa
    40741 aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc atcatgcaaa
    40801 tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt cttgctttat
    40861 cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat attttcgaca
    40921 tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat atcatcgtga
    40981 tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat aatcctggct
    41041 ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt cctttcgata
    41101 tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa aaatatttga
    41161 aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa aatggaacaa
    41221 ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg caccatttat
    41281 gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca cggaatcatc
    41341 agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac tgaattcgaa
    41401 agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc cagcggatca
    41461 tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc aggtatctct
    41521 gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa ggagcaacga
    41581 aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga aaacttgact
    41641 aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta taatcagcct
    41701 caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca atttgaaatt
    41761 gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa cgatagttga
    41821 agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta aagacttatt
    41881 gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat tttctcgtga
    41941 tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata aagacgatgt
    42001 cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt gtacgatcac
    42061 ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt ttccgatgtc
    42121 agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc atatcaaaga
    42181 accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt gaaatcagcg
    42241 ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt tgccgttttt
    42301 gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt gataaagccg
    42361 cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt ttgcataaat
    42421 ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt gtgacacgtc
    42481 ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc accgatttct
    42541 tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac tgaaccatct
    42601 ggatttttca cgcccatatt cacgatactg ccttcatacg gcacattgtc aaaagttgct
    42661 tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa aataatatat
    42721 gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata aatttccctc
    42781 attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga aggaactgtg
    42841 acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc caactcctcg
    42901 gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    42961 ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    43021 tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt
    43081 actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa atattattcg
    43141 ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc agctaaggct
    43201 ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat tgcttttacc
    43261 ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg tttcttcact
    43321 aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc tgggattttg
    43381 aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa ccagaattta
    43441 tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc atcgccgtct
    43501 gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt ttcttgtttt
    43561 tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt atacaaaagc
    43621 atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt ttccgttact
    43681 ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga agcgacgaag
    43741 attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa cacgttcgtt
    43801 aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta tcccccgctt
    43861 tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca agaatcccaa
    43921 agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg attgcctgat
    43981 ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt gcttttgtca
    44041 cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt tgacggaatg
    44101 tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc ataccagaag
    44161 tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa aggatcacga
    44221 tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc gctgtcaaaa
    44281 tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc gcacccgtca
    44341 caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca gaatggaatg
    44401 ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc cctaccatcg
    44461 ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat ttgaatacac
    44521 gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag gcgatgaaaa
    44581 tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca tctcctacac
    44641 tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg gataaaatat
    44701 caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct aaaaccaaga
    44761 caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag ttatcaatga
    44821 tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc cctgacatcc
    44881 ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt tgcaataatg
    44941 gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat gtactaaaat
    45001 taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca gaaagatata
    45061 aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg atcccgttga
    45121 ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg aaagctttca
    45181 tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa aatgtaccaa
    45241 ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata tctacgggtt
    45301 ggttgttact catatttctc cttctttctg aattttttct gaaactacta gaatacttta
    45361 gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga tataattaag
    45421 ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa acgtttgttg
    45481 gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg tgtgtctcca
    45541 cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc tgatgcgaat
    45601 tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa aaaatattta
    45661 gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca aaagaaaatg
    45721 caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat cgacgaacgc
    45781 tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta catcattcat
    45841 gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa agctgactac
    45901 gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag ctttttaata
    45961 agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat atccgaacgg
    46021 tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat atttttttat
    46081 ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc tgtttcccga
    46141 ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc tttttcagta
    46201 ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg aaggtcacgc
    46261 tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa atacaatttc
    46321 agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat ccactctgcc
    46381 aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt ttcacctgtg
    46441 atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc aggataaatc
    46501 tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg attcaattca
    46561 tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag tgtatttttt
    46621 cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat aaatgagtaa
    46681 tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa acatgaaaga
    46741 atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg acaatggttg
    46801 ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc atggcggaat
    46861 ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga cggacggcgt
    46921 attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact ttatgccaaa
    46981 tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac atccagaaat
    47041 cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg aaaaatatga
    47101 aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg gtgatgcagg
    47161 tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag atgatatccg
    47221 tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg aaagagaggt
    47281 tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata gttcctcatg
    47341 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaagagg
    47401 ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat agcttttcat
    47461 attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg tttatcaaga
    47521 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa atagttcctc
    47581 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaaa
    47641 gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga aataatgctc
    47701 ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa aatcagcgtt
    47761 ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt gctgtgtttg
    47821 aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt gatagactgt
    47881 caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac cgattctttg
    47941 ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca ccccagcaaa
    48001 accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta aagctttcct
    48061 aacagattct acttgattct cccaataagt tgatggaatg aattcatgag aatccaagtt
    48121 agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt ttgttgttgt
    48181 gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac catttttttg
    48241 aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt ttacttttct
    48301 aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat aagttccacc
    48361 aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa acggcacacc
    48421 atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat ttaccggttt
    48481 ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta atctatactg
    48541 atagattagt cgtccgataa acactaacag tatgactaca gctattatcg ttcctgcaag
    48601 aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt tttttaccga
    48661 ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga tcatatacgg
    48721 cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa cagacgtgtc
    48781 atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc ccaaaacagt
    48841 caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc gtcgattagg
    48901 taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg ggaaagaggt
    48961 tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat cataaagtga
    49021 aatatttaat tggatcttag gtatattgat acttccgatc agccattcct ctaattggct
    49081 ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag gattgaatcc
    49141 ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt caatttcagc
    49201 tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat ttaaagaatc
    49261 cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta ataaaaacaa
    49321 acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg tcctcccttg
    49381 caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga aaatgaaagc
    49441 cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca aaaaaatagc
    49501 tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    49561 taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct agtcagaaaa
    49621 tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc
    49681 gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa attctgtttc
    49741 aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac tttcacttat
    49801 gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg cctacctctc
    49861 gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat
    49921 atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct catttattca
    49981 cacataaact aattttgggt gctgatgccc cattccatta caatcggttc tatgatgccg
    50041 cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga ttccagcaaa
    50101 gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa ggattgatcg
    50161 tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt ctttatctta
    50221 tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat tgggtaagtg
    50281 taccagtagc cgttttattc atgagtacgt attctattca atactggtct atcaaccaag
    50341 gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt gctgatttcg
    50401 tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt atgacacaga
    50461 tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat ctttattact
    50521 tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt
    50581 attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt gggaataaga
    50641 ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg gatggcagtt
    50701 attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt ttgcttttta
    50761 ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca agtcttgttt
    50821 tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt
    50881 ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta ttgcttctac
    50941 tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt cttttaagta
    51001 tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag catctggaaa
    51061 agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg ccggaagaag
    51121 caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc caaaaaacca
    51181 cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac cggtataacg
    51241 aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt cttttgacaa
    51301 tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc tacgatcgga
    51361 ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat atcgggaccc
    51421 caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa acgccaatct
    51481 atttctattt cattctttct cttactttga ttggctggtt taccttgcta tgtttattta
    51541 tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca tccgatcaaa
    51601 aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt ttttatgtta
    51661 tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag tgatcactga
    51721 tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc attccttcag
    51781 ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca tctattttct
    51841 tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct tgaagaagaa
    51901 ggcaactttc tcttacagta taataacctt gagagtctac tggattattg aaatcaccta
    51961 ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc cactcaaaat
    52021 aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca aaaatgtcct
    52081 gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct tctttctttt
    52141 gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc agtgcatttc
    52201 cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa tactcttttc
    52261 cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg ataggtactt
    52321 gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca gattccagta
    52381 attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc ttatctgcta
    52441 attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg agtgtcatga
    52501 atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg
    52561 tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa gctcaatgga
    52621 ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc tttttcacga
    52681 cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa ccaaacgaaa
    52741 aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    52801 caacctctcc ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt
    52861 gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt
    52921 tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt
    52981 cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc
    53041 ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag
    53101 ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa
    53161 cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag
    53221 gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac
    53281 tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt
    53341 gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga
    53401 ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc
    53461 ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt
    53521 tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc
    53581 ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc
    53641 ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaatac gtaacaacac
    53701 caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag
    53761 ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga
    53821 tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag
    53881 cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg
    53941 ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca
    54001 tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac
    54061 gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa
    54121 gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc
    54181 ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa
    54241 tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa
    54301 acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga
    54361 ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata
    54421 agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt
    54481 ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta
    54541 cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca
    54601 tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg
    54661 tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa
    54721 atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat
    54781 gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg
    54841 gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc aacccagctg
    54901 ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac
    54961 taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat
    55021 agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta
    55081 taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa
    55141 gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt
    55201 atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg
    55261 ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag
    55321 aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt
    55381 actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac
    55441 gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga
    55501 actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac
    55561 cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa
    55621 tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa
    55681 aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac
    55741 tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt
    55801 ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc
    55861 caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg
    55921 gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg
    55981 cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta
    56041 gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa
    56101 acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg
    56161 ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc
    56221 aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca
    56281 caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt
    56341 atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac
    56401 tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg
    56461 tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc
    56521 gaaatggcgc gattgcctat gcaatctaca attatacgaa ttacaccggc gatacggaat
    56581 atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg
    56641 tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat
    56701 atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt
    56761 acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag
    56821 aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag
    56881 gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc
    56941 ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct
    57001 tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag
    57061 aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt
    57121 cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa
    57181 tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct
    57241 tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga
    57301 aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt
    57361 ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta
    57421 ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag tcttattctt
    57481 tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg
    57541 tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag
    57601 cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc
    57661 agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca ctctttttca
    57721 aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag
    57781 acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa
    57841 gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa
    57901 atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa
    57961 cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt
    58021 atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca
    58081 attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact
    58141 tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc cagagatctc
    58201 cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga
    58261 agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg
    58321 aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag
    58381 cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga
    58441 gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc
    58501 tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta
    58561 cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt
    58621 aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa
    58681 tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca
    58741 tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg
    58801 aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga
    58861 gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct
    58921 ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac
    58981 ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa
    59041 tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat
    59101 cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca
    59161 gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac
    59221 cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa
    59281 cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact
    59341 aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg
    59401 cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat
    59461 catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc
    59521 taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga
    59581 tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt tataccaaaa
    59641 ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt
    59701 agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca
    59761 taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt
    59821 cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga
    59881 gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt
    59941 ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta
    60001 tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac
    60061 acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca
    60121 aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca
    60181 tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc
    60241 actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt
    60301 ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct
    60361 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
    60421 attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg
    60481 cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa
    60541 aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa
    60601 tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg
    60661 agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag
    60721 ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt
    60781 tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa
    60841 ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc
    60901 agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg
    60961 atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca
    61021 ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga
    61081 acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc
    61141 cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc
    61201 atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat
    61261 atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg
    61321 atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa
    61381 ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca
    61441 ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta
    61501 aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag
    61561 aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa
    61621 atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga
    61681 gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca
    61741 taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg
    61801 atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa
    61861 gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa
    61921 actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta
    61981 tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc tttcttgaag
    62041 tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac taataatgca
    62101 tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga gaagatcagc
    62161 atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta aggacgttcg
    62221 aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat gtacacgata
    62281 caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac gtcatccatt
    62341 acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac gacatttcta
    62401 gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa tccttcacga
    62461 gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg taaaagcaca
    62521 ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc taggatttga
    62581 cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc tggtacgaca
    62641 tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa agcgataaat
    62701 acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt
    62761 tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc aatatgttcc
    62821 aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga gactagtccg
    62881 actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat atatacccct
    62941 agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt caagatcgtg
    63001 atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt gccaagttct
    63061 acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat taccatacct
    63121 gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct gcccagtgct
    63181 gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag ctcaccggaa
    63241 accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc
    63301 caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc cattgtcaga
    63361 ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt tgacactctc
    63421 cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa
    63481 ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc ttcaaagaca
    63541 acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga tccaaacatg
    63601 cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct tattttgctt
    63661 cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga gctgctttga
    63721 ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc attacgctgg
    63781 cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat cctagttggt
    63841 tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta tgtgaagtta
    63901 agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat acgtcatggt
    63961 tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca tagtctacca
    64021 tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct actttaaatg
    64081 acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca cccattaatt
    64141 taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg
    64201 ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg atatctgcgc
    64261 ctttttcaaa tacatatgtc gcaaagtaag agataacgtc tctcatgcgg atatctttga
    64321 tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca atcgttacag
    64381 attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata taatctttgt
    64441 tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt
    64501 tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca tctacgtgta
    64561 agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac
    64621 cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg attggtgttt
    64681 tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg cgatagttgt
    64741 ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg gcagctttta
    64801 gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct aaatgttttc
    64861 cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg tctaacagat
    64921 tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca gcaactagtt
    64981 caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca taccaaagtc
    65041 cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct ttatagctca
    65101 ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca ggagaagatt
    65161 cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt aaagatggca
    65221 tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa ggaacggaat
    65281 gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc acagtgtgtt
    65341 caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga ggcatgtagt
    65401 tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta tagatttggc
    65461 cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc gacaatgatt
    65521 cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct acattacacc
    65581 tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt taatatgtat
    65641 caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga atttacaaat
    65701 gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta ggatgggttt
    65761 caaaattgga atgttgtaat agtatttccc tttttcaagg taaaaactta ctaaattcta
    65821 atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca caaacttacc
    65881 atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt caccgttaat
    65941 acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt caatttttgt
    66001 ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt tcggcatttt
    66061 accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag agaacaatgc
    66121 ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc
    66181 ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt ttttcgctaa
    66241 gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca ctacatcgcg
    66301 atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct tcggatcaag
    66361 ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca gcatcaatgg
    66421 gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta ggtctaatcc
    66481 agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat cgtatgtttt
    66541 atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg taaatgagat
    66601 tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga tgttgaagtt
    66661 tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt cgtagttgtt
    66721 cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg aaagtgcgtc
    66781 cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc ggccgcttgg
    66841 gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc ctgccaattg
    66901 gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat caccagttgg
    66961 atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc
    67021 gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat tcattttact
    67081 gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa ttaattattt
    67141 cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag ggacgaaatc
    67201 tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa catttaacgc
    67261 tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa agcatgtact
    67321 ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt cgtttcgatc
    67381 ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct ttgtcaacag
    67441 aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct ctaattccga
    67501 acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt tctgcaattt
    67561 atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag gaatgggaca
    67621 agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa aattatttta
    67681 ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt tgtcatccga
    67741 tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa
    67801 ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca aagctacgat
    67861 tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac tgaaacacaa
    67921 cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc tgatcctact
    67981 cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc cttccactct
    68041 caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa ccgttcgatc
    68101 gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat ccaaaaagac
    68161 gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa accattggtt
    68221 ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt tcctagccga
    68281 ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga taaagaagaa
    68341 atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca tcctgatttt
    68401 tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt tttaggttat
    68461 cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat taaagaagct
    68521 aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca tacatggggt
    68581 cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga attgtggcag
    68641 caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt tggtgcagac
    68701 atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa
    68761 ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca attaggtacg
    68821 gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc tgatttagac
    68881 ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga caaagaagca
    68941 cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg acattttatc
    69001 gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt gatcaggact
    69061 tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt agaaattcct
    69121 tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa atccactttt
    69181 ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc ttgcgtatac
    69241 gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt catcaaatcg
    69301 atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact catggcaatc
    69361 agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg ccctaaatca
    69421 ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa atatttaaaa
    69481 atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac tccagcggtt
    69541 cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt
    69601 gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact tttataatat
    69661 atat
//
LOCUS       DAZKPO010000004        68588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000004 DAZKPO010000000
VERSION     DAZKPO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.004"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..688
                     /locus_tag="ACG8NZ_000232"
     CDS             170..688
                     /locus_tag="ACG8NZ_000232"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0537941.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            816..1499
                     /locus_tag="ACG8NZ_000233"
     CDS             816..1499
                     /locus_tag="ACG8NZ_000233"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0537942.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1808..1881
                     /locus_tag="ACG8NZ_000234"
     tRNA            1808..1881
                     /locus_tag="ACG8NZ_000234"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1842..1844,aa:Arg,seq:tct)
     gene            2040..2399
                     /locus_tag="ACG8NZ_000235"
     CDS             2040..2399
                     /locus_tag="ACG8NZ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0537943.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2522..4153
                     /locus_tag="ACG8NZ_000236"
     CDS             2522..4153
                     /locus_tag="ACG8NZ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0537944.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4215..4856)
                     /locus_tag="ACG8NZ_000237"
     CDS             complement(4215..4856)
                     /locus_tag="ACG8NZ_000237"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0537945.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5042..5118
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5138..5446
                     /gene="rplU"
                     /locus_tag="ACG8NZ_000238"
     CDS             5138..5446
                     /gene="rplU"
                     /locus_tag="ACG8NZ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0537946.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5473..5817
                     /locus_tag="ACG8NZ_000239"
     CDS             5473..5817
                     /locus_tag="ACG8NZ_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0537947.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5830..6123
                     /gene="rpmA"
                     /locus_tag="ACG8NZ_000240"
     CDS             5830..6123
                     /gene="rpmA"
                     /locus_tag="ACG8NZ_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0537948.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6557..9364
                     /locus_tag="ACG8NZ_000241"
     CDS             6557..9364
                     /locus_tag="ACG8NZ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0537949.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9556..10047
                     /locus_tag="ACG8NZ_000242"
     CDS             9556..10047
                     /locus_tag="ACG8NZ_000242"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0537950.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10150..11139
                     /locus_tag="ACG8NZ_000243"
     CDS             10150..11139
                     /locus_tag="ACG8NZ_000243"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0537951.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11172..11975
                     /locus_tag="ACG8NZ_000244"
     CDS             11172..11975
                     /locus_tag="ACG8NZ_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0537952.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11996..12910
                     /locus_tag="ACG8NZ_000245"
     CDS             11996..12910
                     /locus_tag="ACG8NZ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0537953.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12993..13367
                     /locus_tag="ACG8NZ_000246"
     CDS             12993..13367
                     /locus_tag="ACG8NZ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0537954.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13506..13751
                     /locus_tag="ACG8NZ_000247"
     CDS             13506..13751
                     /locus_tag="ACG8NZ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0537955.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13923..14987
                     /locus_tag="ACG8NZ_000248"
     CDS             13923..14987
                     /locus_tag="ACG8NZ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0537956.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15233..15658
                     /locus_tag="ACG8NZ_000249"
     CDS             15233..15658
                     /locus_tag="ACG8NZ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0537957.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15651..16100
                     /gene="nusB"
                     /locus_tag="ACG8NZ_000250"
     CDS             15651..16100
                     /gene="nusB"
                     /locus_tag="ACG8NZ_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0537958.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16197..17051
                     /locus_tag="ACG8NZ_000251"
     CDS             16197..17051
                     /locus_tag="ACG8NZ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0537959.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17052..18389
                     /gene="xseA"
                     /locus_tag="ACG8NZ_000252"
     CDS             17052..18389
                     /gene="xseA"
                     /locus_tag="ACG8NZ_000252"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0537960.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18394..18618
                     /locus_tag="ACG8NZ_000253"
     CDS             18394..18618
                     /locus_tag="ACG8NZ_000253"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0537961.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18620..19510
                     /locus_tag="ACG8NZ_000254"
     CDS             18620..19510
                     /locus_tag="ACG8NZ_000254"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0537962.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19519..20337
                     /locus_tag="ACG8NZ_000255"
     CDS             19519..20337
                     /locus_tag="ACG8NZ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0537963.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20444..20893
                     /locus_tag="ACG8NZ_000256"
     CDS             20444..20893
                     /locus_tag="ACG8NZ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0537964.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20911..22593
                     /gene="recN"
                     /locus_tag="ACG8NZ_000257"
     CDS             20911..22593
                     /gene="recN"
                     /locus_tag="ACG8NZ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0537965.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22648..22764)
                     /locus_tag="ACG8NZ_000258"
     CDS             complement(22648..22764)
                     /locus_tag="ACG8NZ_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0537966.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22837..23793)
                     /locus_tag="ACG8NZ_000259"
     CDS             complement(22837..23793)
                     /locus_tag="ACG8NZ_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0537967.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23964..24332
                     /locus_tag="ACG8NZ_000260"
     CDS             23964..24332
                     /locus_tag="ACG8NZ_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0537968.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24502..24933
                     /gene="mraZ"
                     /locus_tag="ACG8NZ_000261"
     CDS             24502..24933
                     /gene="mraZ"
                     /locus_tag="ACG8NZ_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0537969.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24954..25913
                     /gene="rsmH"
                     /locus_tag="ACG8NZ_000262"
     CDS             24954..25913
                     /gene="rsmH"
                     /locus_tag="ACG8NZ_000262"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0537970.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25918..26319
                     /gene="ftsL"
                     /locus_tag="ACG8NZ_000263"
     CDS             25918..26319
                     /gene="ftsL"
                     /locus_tag="ACG8NZ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0537971.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26320..28512
                     /locus_tag="ACG8NZ_000264"
     CDS             26320..28512
                     /locus_tag="ACG8NZ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0537972.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28538..29500
                     /gene="mraY"
                     /locus_tag="ACG8NZ_000265"
     CDS             28538..29500
                     /gene="mraY"
                     /locus_tag="ACG8NZ_000265"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0537973.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29514..30875
                     /gene="murD"
                     /locus_tag="ACG8NZ_000266"
     CDS             29514..30875
                     /gene="murD"
                     /locus_tag="ACG8NZ_000266"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0537974.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30901..31989
                     /gene="murG"
                     /locus_tag="ACG8NZ_000267"
     CDS             30901..31989
                     /gene="murG"
                     /locus_tag="ACG8NZ_000267"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0537975.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32004..33224
                     /locus_tag="ACG8NZ_000268"
     CDS             32004..33224
                     /locus_tag="ACG8NZ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0537976.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33380..34705
                     /gene="ftsA"
                     /locus_tag="ACG8NZ_000269"
     CDS             33380..34705
                     /gene="ftsA"
                     /locus_tag="ACG8NZ_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0537977.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34728..35969
                     /gene="ftsZ"
                     /locus_tag="ACG8NZ_000270"
     CDS             34728..35969
                     /gene="ftsZ"
                     /locus_tag="ACG8NZ_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0537978.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35986..36663
                     /locus_tag="ACG8NZ_000271"
     CDS             35986..36663
                     /locus_tag="ACG8NZ_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0537979.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36677..37276
                     /locus_tag="ACG8NZ_000272"
     CDS             36677..37276
                     /locus_tag="ACG8NZ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0537980.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37357..37578
                     /locus_tag="ACG8NZ_000273"
     CDS             37357..37578
                     /locus_tag="ACG8NZ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0537981.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37627..38409
                     /locus_tag="ACG8NZ_000274"
     CDS             37627..38409
                     /locus_tag="ACG8NZ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0537982.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38430..39137
                     /locus_tag="ACG8NZ_000275"
     CDS             38430..39137
                     /locus_tag="ACG8NZ_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0537983.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39399..42197
                     /gene="ileS"
                     /locus_tag="ACG8NZ_000276"
     CDS             39399..42197
                     /gene="ileS"
                     /locus_tag="ACG8NZ_000276"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0537984.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42246..43772)
                     /gene="zwf"
                     /locus_tag="ACG8NZ_000277"
     CDS             complement(42246..43772)
                     /gene="zwf"
                     /locus_tag="ACG8NZ_000277"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0537985.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43787..44434)
                     /locus_tag="ACG8NZ_000278"
     CDS             complement(43787..44434)
                     /locus_tag="ACG8NZ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0537986.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44618..44947)
                     /locus_tag="ACG8NZ_000279"
     CDS             complement(44618..44947)
                     /locus_tag="ACG8NZ_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0537987.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45124..45852
                     /gene="sfsA"
                     /locus_tag="ACG8NZ_000280"
     CDS             45124..45852
                     /gene="sfsA"
                     /locus_tag="ACG8NZ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0537988.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45868..46881
                     /locus_tag="ACG8NZ_000281"
     CDS             45868..46881
                     /locus_tag="ACG8NZ_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0537989.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46881..48158
                     /locus_tag="ACG8NZ_000282"
     CDS             46881..48158
                     /locus_tag="ACG8NZ_000282"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0537990.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48221..50923
                     /locus_tag="ACG8NZ_000283"
     CDS             48221..50923
                     /locus_tag="ACG8NZ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0537991.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51075..51392
                     /locus_tag="ACG8NZ_000284"
     CDS             51075..51392
                     /locus_tag="ACG8NZ_000284"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0537992.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51422..51742
                     /locus_tag="ACG8NZ_000285"
     CDS             51422..51742
                     /locus_tag="ACG8NZ_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0537993.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51850..53310
                     /locus_tag="ACG8NZ_000286"
     CDS             51850..53310
                     /locus_tag="ACG8NZ_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0537994.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53378..53599
                     /locus_tag="ACG8NZ_000287"
     CDS             53378..53599
                     /locus_tag="ACG8NZ_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537995.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53630..53812
                     /locus_tag="ACG8NZ_000288"
     CDS             53630..53812
                     /locus_tag="ACG8NZ_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0537996.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53812..54225
                     /locus_tag="ACG8NZ_000289"
     CDS             53812..54225
                     /locus_tag="ACG8NZ_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0537997.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54348..55529
                     /locus_tag="ACG8NZ_000290"
     CDS             54348..55529
                     /locus_tag="ACG8NZ_000290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0537998.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55600..55764)
                     /locus_tag="ACG8NZ_000291"
     CDS             complement(55600..55764)
                     /locus_tag="ACG8NZ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0537999.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55918..55991
                     /locus_tag="ACG8NZ_000292"
     tRNA            55918..55991
                     /locus_tag="ACG8NZ_000292"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55952..55954,aa:Arg,seq:cct)
     gene            complement(56060..57199)
                     /locus_tag="ACG8NZ_000293"
     CDS             complement(56060..57199)
                     /locus_tag="ACG8NZ_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0538000.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57371..57505
                     /locus_tag="ACG8NZ_000294"
     CDS             57371..57505
                     /locus_tag="ACG8NZ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538001.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57498..58133)
                     /locus_tag="ACG8NZ_000295"
     CDS             complement(57498..58133)
                     /locus_tag="ACG8NZ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538002.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58246..58881)
                     /locus_tag="ACG8NZ_000296"
     CDS             complement(58246..58881)
                     /locus_tag="ACG8NZ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0538003.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58915..59376)
                     /locus_tag="ACG8NZ_000297"
     CDS             complement(58915..59376)
                     /locus_tag="ACG8NZ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0538004.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59506..59937)
                     /locus_tag="ACG8NZ_000298"
     CDS             complement(59506..59937)
                     /locus_tag="ACG8NZ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0538005.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59955..60275)
                     /locus_tag="ACG8NZ_000299"
     CDS             complement(59955..60275)
                     /locus_tag="ACG8NZ_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0538006.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60574..61350
                     /locus_tag="ACG8NZ_000300"
     CDS             60574..61350
                     /locus_tag="ACG8NZ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0538007.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61365..61568
                     /locus_tag="ACG8NZ_000301"
     CDS             61365..61568
                     /locus_tag="ACG8NZ_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538008.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61584..61922
                     /locus_tag="ACG8NZ_000302"
     CDS             61584..61922
                     /locus_tag="ACG8NZ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0538009.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61909..62088
                     /locus_tag="ACG8NZ_000303"
     CDS             61909..62088
                     /locus_tag="ACG8NZ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538010.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(62131..62601)
                     /locus_tag="ACG8NZ_000304"
     CDS             complement(62131..62601)
                     /locus_tag="ACG8NZ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538011.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62688..63386
                     /locus_tag="ACG8NZ_000305"
     CDS             62688..63386
                     /locus_tag="ACG8NZ_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0538012.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63564..63905
                     /locus_tag="ACG8NZ_000306"
     CDS             63564..63905
                     /locus_tag="ACG8NZ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538013.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63898..64569
                     /locus_tag="ACG8NZ_000307"
     CDS             63898..64569
                     /locus_tag="ACG8NZ_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0538014.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64575..65267
                     /locus_tag="ACG8NZ_000308"
     CDS             64575..65267
                     /locus_tag="ACG8NZ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0538015.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65264..66013
                     /locus_tag="ACG8NZ_000309"
     CDS             65264..66013
                     /locus_tag="ACG8NZ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538016.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            66025..66294
                     /locus_tag="ACG8NZ_000310"
     CDS             66025..66294
                     /locus_tag="ACG8NZ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538017.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66300..66464
                     /locus_tag="ACG8NZ_000311"
     CDS             66300..66464
                     /locus_tag="ACG8NZ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538018.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66457..66756
                     /locus_tag="ACG8NZ_000312"
     CDS             66457..66756
                     /locus_tag="ACG8NZ_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538019.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66756..67058
                     /locus_tag="ACG8NZ_000313"
     CDS             66756..67058
                     /locus_tag="ACG8NZ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0538020.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            67098..67463
                     /locus_tag="ACG8NZ_000314"
     CDS             67098..67463
                     /locus_tag="ACG8NZ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538021.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67953..68093
                     /locus_tag="ACG8NZ_000315"
     CDS             67953..68093
                     /locus_tag="ACG8NZ_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538022.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            68090..68272
                     /locus_tag="ACG8NZ_000316"
     CDS             68090..68272
                     /locus_tag="ACG8NZ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538023.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68285..68479
                     /locus_tag="ACG8NZ_000317"
     CDS             68285..68479
                     /locus_tag="ACG8NZ_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538024.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT        22867 a        11442 c        14394 g        19885 t
ORIGIN      
        1 ttttttaaac taacgtcaat tatttataat aaaaaatgag ataaagatga ggaaatagaa
       61 ttagaaacat ataattatta aagggaaaca tttttacagc tataatttga aaaaatttat
      121 cgttattata tgattaagaa ggattgaata aagaaagaag ttgaaaacga tacctgatta
      181 caaaaaaata acttttataa gttctataac gttgattata tttttaattc ttattcttct
      241 cgtcgtcttg gttccccgaa tttttggttt gactccttat attattgagg acaaagaaat
      301 gagtccatat tatgcacaag gaaatctgat atatgtacga ccgaaaaata caaatgagat
      361 tttagtcgga gatattatta catattatga aaattcagga aaaaaaatct cgacgcgtcg
      421 cgtaatagcc gttgaaaaca accacgaaga tttttataca aaagctgata caaagactta
      481 tattgagcct ggagtagtaa aaaagagaaa tattatcggt agtcccatct ttcagattcc
      541 atatattgga ctagtccttt caaaaacggc atttgcatgg attcaggggt tattctttac
      601 gattgctttt tgcctaacag gaatcacagc atggaatacc tttgtagagt tgaaaaagaa
      661 gcggagacga acacagaaat ttttgtaaga atgttaaaaa cataaatgta ttccagagat
      721 ccaacgactc ttctgaacat gaaacacgag aaaacactca tgcagcttgt agatttagtc
      781 agttgttcgt ataatgtgtg aagaggagga gtagtatgcg caatgaaatg atgcatcgtc
      841 ctgtagtcaa agaagaacta ttggatttta tgcgtaccca gcaaaaaaaa ttaccgggtg
      901 agctaggcaa actggaagaa gaagctcatg tagcagaagt accagttatt ccccatgaaa
      961 cagttgtatt tttgaaattt ttacttggac agcttcagcc tgaacgaatc ttggagatcg
     1021 gagcagctat cggtttttca tcaagtgtca tggccacgac aatcggagaa aacgctcatg
     1081 ttacgacaat tgatcgtttc gatgtcatga tcgagaaagc taaaaaaaac tatgaacgtc
     1141 taggactgac agataaagtg actttgcttg aaggtcaagc agcagatatc ttgccggagt
     1201 tgtctggtcc gtatgacttt atttttatgg atagtgcaaa atcaaaatat attgaatttt
     1261 tacctgaatg cttacgtttg ttaagaaaag gtggcgtttt gatggtagat gacatcttcc
     1321 aaggaggaac gatcttgctc ccagatgaag agattccccg tggcaaacga gctatccatc
     1381 gtaaattaaa tgaatttctt cgtgttgtga tggatcatcc cgatttgaca tcgacaatct
     1441 tgccattagg tgatggtgtg atcttaatga caaaggaatc agaaacaatc gagttgtgat
     1501 acatgattat ggcagcctgt agataatcga ttgaatcgag ggctcaattc gaacttgatg
     1561 taaaagtttc tatatttgta agtcaattaa aggatacagg aaatctaaat aaacaggagt
     1621 agcatgaagt caatgtagct cctgttttca attgataacc ttagaacagc tgaaggagaa
     1681 tgtctcgttt taaaagattg atttagtagt tgggccaaaa taattcatta tttgctaatt
     1741 aaataactat ttcgtattga cagaagcaat ttaattatct ataattattg atgttccgct
     1801 gattcttgtc atagtagctc agctggatag agcatccgcc ttctaagcgg acggtcgggg
     1861 gttcgaatcc ctcctgtgac gctaaaaaac cgttagaaac tgaggtttct aacggttttt
     1921 ctttgttttt tgtgccaata ttgtttgttt tcaaaggcaa ataaaataaa aaaacatcct
     1981 tatcgagtac aattataagg agcaaaaaaa taaaactcat cagaaaagag gaagaagata
     2041 tgacgatact atctagaatt ttagtgactc tagttgcaat tgaatttttt tatatcatgt
     2101 atatcgaaac ggtgagaact gattctgaca cgacaagtag ggtttttaaa atgagtaaag
     2161 aagagttaag cagaaaatct gttcaaacat tgtttaagaa ccaaggagtg tacaatggac
     2221 tattaggagt gggactactt tatggagctt atctttcttc cgcatctaaa gaaatcacaa
     2281 gtatgctatt aatatctatt ttttttgttg cattatatgg cagtttgact agcgatcgtt
     2341 ggattttact caaacaaggt gggctagctc tgttggcatt gctttctatt ttcttataaa
     2401 tatagaaaaa ataagaattt atctaacagg aaactttgtg aaaaatatta ctttatctca
     2461 tgaaatatga tattctaatg aatggtattt tagaaaattt catgaaaaag gagttagata
     2521 ggtgttcaaa aattatgttt cctcactaaa aaaagaattt tcaggttatg atctttcaag
     2581 atttcagaaa gatcttttgg ctgggatcac agtagctgct gtagcattgc ctttagcctt
     2641 ggcttttggg gtatcaagcg gagctgatgc ggccgcaggt ttgattacgg ctatcattgc
     2701 cggtatcgtt attggcggtt tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc
     2761 gatggcagct atcttgatgt ctattgcggc aacaaaggga atgcaaggtg tattgcttgc
     2821 tactttttta gcgggtctat ttttacttgc agcaggactt ttccgattag gaacactgac
     2881 ttcttttatc ccagcacccg taattacggg gtttacttca ggtattgcta tcattattgc
     2941 attaggtcag atcgataatt tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa
     3001 actaagtagt tatcaaacga taggatttcc gataaacaca gcctctttga tcatgggagg
     3061 gctagtcatt ttagggatgt ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc
     3121 gctgctggct atcattttgg caacagcagt tatgctattg ttccatttac cagtagcaac
     3181 tgtgggtaag attccacaaa cattgatcag tagtaatcgt ttgaatatgg gagattttag
     3241 cttttctgcc ttgcaacaag tggcagttcc agctattagc attgctttat taggaatgat
     3301 tgaaagcttg ctatgtggtg cttcggctgg ccgaatggca aataagccct tagatagcaa
     3361 tcaagaatta atggcgcaag gaattggaaa tctgttgctt cctttctttg gcgggattcc
     3421 tgctactgcg gccatcgccc gtacaagcgt ggccatcaaa tctggtgcac aaactagatt
     3481 agctggtatg atacatgcag ttgtcttact gctctccatg cttgtttttg cgccagtgat
     3541 gtcacagatt ccaatgccag cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa
     3601 tgaatgggaa acgatcaagg agctatttac taataaatat tggtcagcag ttctattgtt
     3661 tatccttacg atgatttgta cagttatctt tgatttaagt attgctatcg tgatcggggt
     3721 gatcggcggc tgtgtatttt ttgttgtcaa aagcgctgct atcacgattt cattagaaac
     3781 gatcgattgg caaagaatgg ggctgccaga aacggaaaag ctgaataact ggacagtagt
     3841 gtatattagt ggtccactgt tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga
     3901 actaaatcat cacgatggtg tcattttctc catgcgaggc gtaccaagta ttgatttgac
     3961 ggcacgtgca gtttttgatg aatttcaaga aaaagcagag caaagaggac aaacgattat
     4021 ctatacttcc ttgcagccag aagtagaaaa acaactttct cacttgtggg aacatacatt
     4081 cacgcatcaa catcaaacag ttgctcatgc gttgacagct ttacatgcga aatatactgc
     4141 aattagccat taaaacaatg aaaaagaaca gacaagactg taaactgaag attttatggt
     4201 cttgtctgtt ctttttaata ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg
     4261 ataacaggta caagggagcg attgtagaaa atagctgctg gatgaaaaag tggaagaatt
     4321 cgatagtttt tttctgacca ttcgtaggca tccttttgtt ctgttaattg taagacaggc
     4381 cctgtataga gttgaccatg atttttggta accgtatatt ctttgccaag taaacgttga
     4441 agaccaatgt ttccaagtgt cgcaattaat tttggctgga ccttcatcag ttcgtaatcc
     4501 aatattggtg catgcgcaaa tacttcggaa cgagtaggtg tccgatttgg ataaacagtt
     4561 tctgactgtt gtgtccgttt attgatccga tgagtcacgc gatacgggcg gctccttaca
     4621 gcactggtga tatacacctc ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat
     4681 tcttttccag cttgaccgct aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct
     4741 tctcccacga gcatcagttt aggatgaaga ggtccttgcc cagcgataaa tccttctaca
     4801 ggacggccgt tcattcttaa agaagcttct tgtatcagtt cttttgggta ttccatttat
     4861 gctcctcctt tttcttttat tataagaata aaagaaacac aatgcgacta ttttatttgg
     4921 tagaactgtg aggatagaaa agcagaagta ttgcttgaat ttttgtttta ctttttgatt
     4981 ttaaatttag ttgactaacg gttgctgttc tggtaagcta gtactgttgt aattgtggac
     5041 tccacagcta caaccgctca gaacaagtaa taagcacagc atatgctgtc acttcggatg
     5101 gcgagtctaa gtatatcaga aggaggtgct ttatagcatg tacgcaatta ttaaaactgg
     5161 tggtaaacaa gtaaaagttg aagtgggaca agcaatctat gttgaaaaac ttgacgttga
     5221 agcaggagaa aaagttgttt ttgacgaagt tatcttagta ggcggagaat ctacaaaagt
     5281 aggcgctcca actgttgcag gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca
     5341 aaagaaagtc gttactttca aatacaaacc taaaaaacat tcacaccgta aacaaggtca
     5401 tcgtcaacca tatacaaaag tggttatcga cgcgatcaac gcttaatagc gtttgagaaa
     5461 gaaggcctat atatgatcaa aggaacattt aagcgaaatg acgcaggtca gatcgtttca
     5521 ttcactttga ccgggcatgc tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt
     5581 gtttctgcgt tagcgatcag cactgtcaac gggattgcct cattagccgg ttttgagccg
     5641 attgttgaaa tgaatgaaga agaaggcggt tatctttata ccgaagtcac ttcaggaatg
     5701 acacaagaac aaaacaatat tgctgagatt ctattagaaa atcttttatt aggattacaa
     5761 tcgatagaag ctgaaaactc agaatatatc caaattaaaa caattaatga taaataggag
     5821 gtgcagacca tgttgttatc tatgaatcta caattatttg cccacaaaaa aggtggcggt
     5881 tctacatcta acggccgtga ctcagaatca aaacgtctag gtgctaaacg tgctgatgga
     5941 caaacagtta caggtggatc aattctttac cgtcaacgcg gtacaaaaat ctatccaggt
     6001 gtaaacgtgg gtatcggtgg agacgacact ttatttgcaa aagttgatgg cgtagtacgt
     6061 ttcgaacgta aaggccgtaa caaaaaacaa gtgtctgttt atccagtagc tcaagaagct
     6121 taattgaatc ttatgattaa agaggttgtg acaagacttt tgtcacagcc ttttttgttt
     6181 ataaaaataa aagttgtagt ggaggctacg aagtttactt tgtcttttta cacttaatat
     6241 ttttttgcca atttggaatc aggatgtttt gataactatc gttttcaatt gatggaagct
     6301 atattgtttg ctatcagtga aataattata tggtaaacag tgttcttttt cttgtttcca
     6361 ttataaaaat cgtatcggga tttttcgttc ttttaaattg aacatttcta tctatccgcg
     6421 tgttatcaac aaaacaaaat caggtaaata gttgacgaaa cagaaataaa tacatagtat
     6481 tatgttttga agcctaagta caatagtcta tagaaaagga gcgataccat tggcaaaacg
     6541 cattgaccga atttatatgt atgtcaaaga aaaaaccgct catttaacaa aggcagaata
     6601 tgatcaagga gtaacaacac aggaaattgc tgaagtgtta gggattcagc gaacaaatag
     6661 cagtaaagat ctgaatcagc tagtgcgcga aggaaaactg ataaagacag acggacgacc
     6721 agtacgttac atatatcaaa cactcgttac gcacgtaaag ccagttacaa aacatgtagt
     6781 cagttacaaa gaaacgccgg tctctgaaga aaaaaataca ctccaaatag atactaagga
     6841 tatctttgca aaaatcattg gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa
     6901 agctgcgatt ttgtatccgc caagaggatt aaattgtttg attacaggac ctaccggatc
     6961 aggaaagact tattttgcac acgcgatgta tcattttgca aaagcgcatc atgtaataga
     7021 tgaggaaacg gaactgattg ttttcaattg tgctgattat gccaataacc ctgagctttt
     7081 gatgagtcat ttatttgggt acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat
     7141 ggggattatc gatcaagcgg atggcggtat gctgtttttg gatgagattc accggttgcc
     7201 gcctgaaggt caagaaatga tcttttactt tatggatcat ggaacttaca gtcgtcttgg
     7261 ggaaacaacg aaatcccatg aagcaaatgt acggatcgta ggtgcgacca cagaagaccc
     7321 aggttcttct ctattagaaa cttttgttcg gcgtatccca atcaatatca aacttccggc
     7381 ttttgaaaaa cgtcctgcaa atgagaaagt cgatttagta aaaatcatga ttgcccacga
     7441 agcaaaccgc acgcagcgaa agatttcttt gacagaagat gtagcaaaag cattgatcgg
     7501 cagtgtgacc tatggaaata taggacaatt gaagtcaaat atccagttag tatgtgcacg
     7561 aggatttcta aatcatatgc aatcacccga aatctcgatc acaatcgatg atttgacaga
     7621 agggattcgt agcggattga ttcaattagc aagcaaccgc agtgcaatgg cagagctttc
     7681 taaaatattg gagccaaaga taactgtagc accaaatgat acgatcatga aaatccaatc
     7741 ggactcctat gagttacctt ataatctata tgatatcatc ggagataaag cagctttact
     7801 gaagtcggat ggtttggatc aagaagcaat caatcatttc atttctacag atatcaacat
     7861 ccatctgaag tctttctata aagaccatgg tttttctttt aatgcagaca gcaaattagc
     7921 agaatttgta gatccgaaaa tcatagaagt gaccaatcag atttatgtaa tggtcaaaag
     7981 atctttacca tatgagtttc agcaaaactt tatttatgct atgagcttgc atatcagttc
     8041 ttttttgaaa aaaatcaact taggagaaga acggcatacg aatgataaca tccgtgagat
     8101 ggcttttgat tttccagatg aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac
     8161 gtattttcaa gtggagattc cagaaagtga agactattac ttgaccgttc tactcgtctc
     8221 gttacgggca aatcaagcaa gcggcagaat cggtgtagtc gttgcagctc acggaaacag
     8281 cacagcaagc agtatggtcc aagttgtcca acagttgtta gatgcagaga acgtacgagc
     8341 agtagacatg cctttggata tggatccgaa aacagcattg gaccgaattg aacgcaatgt
     8401 acaagaagta gatgaaggaa gcggggtcat actcttagta gatatggggt cccttgcgtc
     8461 gtttaattcg caaattcaaa gagacactgg aattccagta cgtactgttg atatggtaac
     8521 aacttctcta gttttggaaa ctgtacgaaa ggtctcagta ttagggacag atctagatat
     8581 gttgcatgaa tcattgaaaa attttcgagg ttacgcagaa atatcttctg tagaatctgt
     8641 tcaggaagca aaacctattc ataagaaaaa agctgttttg gctatctgcg catctggcga
     8701 aggtacagca caaagaatca aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac
     8761 agaattagaa gttctagctt tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca
     8821 aaaagaatat cagatcatag ctactactgg gattactgat ttgaatatcc aagccccatt
     8881 tattccttta gaacgtttta ttgatcaaaa tatcgaagtc attttggatc agttgctgat
     8941 ggaatcagaa ttggaggaaa ctgaattcat ctccctagat gaagagtcag ctaaaaatac
     9001 gtgtgttgag tttatctctg ataacttcat ctttattaat ggtagtaagt taatcgatcc
     9061 aatgtggcaa ttttctacac aaatctctca gacgacggga atcggcgatg aagaatatgg
     9121 attcaaaatc aacctagtga tgcacacagc aggaatgatc gaacgtatta ttcggaatga
     9181 accattgact gtggaagaaa acgaattgac aaatacaacg aatgatcctc tttatagcca
     9241 attggcagcc tcagttgttc tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga
     9301 aatgtattat ctgctacgtc tagtccataa tcagttagat aaaaaagaat acactgtgcc
     9361 atgaatacac aaaaacgata cacttttata gtgtatcgtt tttgtgtatt ggttaagaat
     9421 cctttaaatt caattagtgt atcgtaaaaa aagttggcac gctttttgct tatagtagtg
     9481 tgtgctagga ggtggttgtt cacacagaaa tgataggggt ttctgtgaag tagtgaggaa
     9541 gaaggagttt tgcagatgga tattcgattg gcacgtatag atgatcgttt aatccacggg
     9601 caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat
     9661 gtagtcgctc aagataaatt gagaaagttt ctactaaaag aagctgcacc gccggggatc
     9721 acagcaaatg taataaccgt gcagaaaatg attcaaatct atcatagtgg attattatca
     9781 cgagcaaaag tgatgctttt attcaccaat ccccaagatg ttgaagcggt tgtccgctgc
     9841 ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt ttactgaagg caaatcaatg
     9901 attaccaatt ttgtttcagt aaatcaacaa gatattgatt cctttcgtta cttgaacagt
     9961 caagaagtgg agttagagat tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa
    10021 ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt tggaaaaggt caaataattc
    10081 tgttgtaatg gttagcaatt attaatattt ttgaatttgt tacaacaaaa gtaaatagga
    10141 ggtataaata tggtagggat tatcctagca agtcatggac aatttgctga aggaatctta
    10201 caatccggtt caatgatctt tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca
    10261 agtgaaggtc cagatgactt gagagccaaa ttggaagaag cggttgcttc ttttgataat
    10321 caagacgaag tgttattctt agttgattta tggggcggaa caccgttcaa tcaatcaaac
    10381 acattattcg aagagcacaa agataaatgg gcaatcgtca gcggtttgaa tttaccaatg
    10441 ttgattgaag cctacgcatc acgcttttca atggaatctg ctcatgaaat tgcagcacac
    10501 atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa
    10561 gcaccgaaag cagcagttga agacgcacaa cctaaaggag cacttccaga aggaactgtg
    10621 gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg actcacgtct gttacatgga
    10681 caagttgcga cagcttggac aaaagcagtg caaccaaacc gtattatcgt tgtttcagat
    10741 gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta
    10801 aaagcaaatg ttattcctat tagcaaaatg atcgaagtag caaaagaccc tcgttttgga
    10861 aataccaaag cattgctttt atttgaaaat cctgaagacg tattgacagc tgttgaaggt
    10921 ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt
    10981 gtaagtaaag tattgtctat gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa
    11041 aaaggtgtga aattcgatgt acgtaaagta cctaatgatt cacgcgataa catggatgat
    11101 attttgaaaa aagcaaaagc tgaattagca aaagcgtaat aaataatatt aaaatttata
    11161 ggaggcttat catgtctatt atttcaataa ttttagtcgt attagttgcc tttctagctg
    11221 gtatggaagg tattcttgat gaatttcaat ttcatcaacc actagtggca tgtacattga
    11281 tcggtttagt taccggtaac ctcgaagctg gtatcgtact tggcggtacg cttcagatga
    11341 tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc agatgctgca ttagcatcag
    11401 tagcatcagc aattatttta gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga
    11461 tcgcgattgc cgttcctttg gctgttgcag ggttgttctt aacaatgatc gtacgtacaa
    11521 tcgctgttcc aatcgttcat ttgatggatg cggctgctga agaaggaaac atccgtaagg
    11581 ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag
    11641 cagcgttgct atttatccca gcacaaactg tgcaatcatt ccttgaatca atgccagcat
    11701 ggttaacaga tggtatggct atcggtgggg gaatggtcgt agctgtaggt tacgctttgg
    11761 taatcaacat gatggctaca aaagaagtat ggccattctt cgttattggt ttcgttgtag
    11821 ctgcaatttc tcaattaaca ttgattgctc taggtgcttt aggtgttgca ttagctctta
    11881 tttacttgaa cctttctaaa atgggtggtt catcaaataa tggaggcgga agcaactctg
    11941 gtgacccttt aggcgacatt ttgaatgact attaatcttg aaggaggaaa ataaaatggc
    12001 agaagaaaaa atcaaattat caaaaaaaga tcgtttagct gttgcgtggc gctctacctt
    12061 cattcaaggt tcatggaact acgaacgtat gcaaaacggt ggctgggcat tttctatgat
    12121 tccagcaatc aaaaaattat acaaaacaaa agaagaacgt tctgcagcat taaaacgtca
    12181 cttggaattc tttaatacac acccatacat tgcttctcca attttagggg ttacattggc
    12241 tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat gtagcgattc aaggggttaa
    12301 agtaggtatg atgggtcctt tagctggtgt tggtgaccca gtgttctggt ttacagtacg
    12361 tccaatgcta ggtgcactag gtgcttcatt agctatggga ggaaatatcc ttggaccaat
    12421 catcttcttc ttaggttgga acattattcg ttgggcattc atgtggtata cacaagaatt
    12481 tggctacaaa gctggttcta aaattactga tgacctttct ggcggattgt tacaagacgt
    12541 aacaaaaggt gcttcaattt tagggatgtt cgtattagct gcccttgtac aaagatgggt
    12601 atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta gataaaggtg cttacatcga
    12661 atgggataaa ttgccttcag gtggtgaagg aatcaagagt gcatttgaac aagttaataa
    12721 tggcttagcc ctttctcctg aaaaggtaac aacattacaa gacaacttag accaattgat
    12781 tcctggttta gctgcattag gattaacatt gttatgtatg tggttgctta agaaaaaggt
    12841 ttctccaatc gttatcatcc ttggtttatt cgtagtcggt gttgttggac acgtcatcgg
    12901 acttttataa tccataaaaa tatagctcgg gcgttgtccc gagctttttt tatatagtat
    12961 actgttcata aacaaatatc tggagggaga aaatggttca atcaatcaat acaaaagtag
    13021 acttggtaat tgatgctact tcgtttacgg gtctgacaga ttacgggcaa atcatgatag
    13081 gtaataatgg ttttgagttt taccatgcgc gagatagacg caaatatatc caaattccat
    13141 gggaagaagt ggattatgta ctagcatcag tcatgctaaa gggaaaatgg attcctcgat
    13201 atgccattca aacgaaaaaa aatggtacat acacattttc ttccaaagaa cctaaaaaag
    13261 tgcttcgtgc gatcagagaa tatgtagatg aagatagaat ggtacaatca ttgagtttct
    13321 ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa
    13381 cttctaagag gaggttttct ttattttttt actttgcaga agaaaatatg aaatagcaaa
    13441 accaattatt tgtccccttt aaatcccata tgctaaaatt gattagcaaa gaaggaggtt
    13501 atattatgag ttggttatgg acattgattg ttggagccgt gatcggagca attgcaggtg
    13561 cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa tatcattgct ggactagtcg
    13621 gttcaagtat tggacaagcg attttcggcg aatggggacc aaaagctgca ggtatggcac
    13681 ttattccatc tattttaggc gcagtgattt tggtagcagt tgtctcattt ttctttggaa
    13741 aacgatcata agtttttaca aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc
    13801 ctttttggct ttatttattt aaaataaaaa gaagttttcc ggaaattttt atttaactgt
    13861 cgtgtacgaa gaaaagtttg ctataatgga aaaaagcgtg caaagaaaga gggagtttca
    13921 ttatgatgct acgtgtagaa aaattgcgca aaaaaatgca agaagaaaat ttggattcat
    13981 ttttggttac cagtccgtac aatcttcgtt acttaacgaa ctttacaggt acgacaggat
    14041 tagctgttat tactttggaa aaagcatttt ttattactga ttttcgttat accgagcagg
    14101 cggcagcgca ggctcaaggg tttgaaatta ttaaaaatgt cggacctatt tttgatgagg
    14161 tagcaaactt agtacgcaaa gaagaactgc actcattagc ttttgaggaa acaacagtct
    14221 catttttaga atattcggtt ttagaagaaa tcatcgatgc ggaattagta cctgtttcac
    14281 agatgatcga agaactcaga gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag
    14341 catgtcatat tgcggatatg gcatatgatc acatcctgaa aatgatccaa ccgggcatga
    14401 ctgagattga agtcgcaaat caactagact tttatatgcg ttctcttggc gcttcaggtg
    14461 tttcttttga tacgatcgtt gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa
    14521 gcaaaaaaat catcgaacaa ggtgacttga tcacattgga ctttggttgc tattacgaag
    14581 ggtacgtttc cgatatgaca cgtacattcg ctattggtga tcctggggaa aaattaaaag
    14641 aaatttatca aatagtctta gatgctcaat tagcagttat tgatgctgca aaaccaggaa
    14701 tgactggtgt ccaattagat gcagttgcta gagattatat ttcaaaacat ggttacgggg
    14761 aagcatttgg acattctaca ggacatggga tcggtttaga gatccacgaa ggaccaaacg
    14821 tttcttctcg tgcagaaaag caatttgtac caggaaatgt gatcactgat gaaccaggga
    14881 tctacttacc aggaatcggt ggagtacgta ttgaggatga cttgttgatc acagaagatg
    14941 gaaatcgtgt gttgactcgt tcaccaaaag aattgatcat cttatagttt catcaataga
    15001 agaagtaagc gtaaaaatca gtgttcacag actttgttat agaaaatcac tcgatttaat
    15061 gatacactat agaagaactt ttaagctgta agagataata aagttgggtg ttttctccaa
    15121 ttcatagcag tttgcagctg atcggcggca gttttcttac cacgtgatca ctatgccgca
    15181 tcatagagaa ttcggatgta tctcgtgctt aggcaaaggg aggaataaat aaatggcaga
    15241 tgaaaaaaat ttgattttaa acgcaaacca agatttagga gagatcgtga tcgctcctga
    15301 agtcatcgaa gtaattatcg ggattgcagc ttctaaagta gaaggcgtat acggaatgcg
    15361 cggaactttt gccagcaatg tcactgaatt attaggacgt gcagctcacg gaaaaggtgt
    15421 ttacttagca aacgaggaag atggcttgaa agtagatctt tactgttatc tagaatatgg
    15481 tgtttcagtt cctaaagtag caatggaaat gcaggaacgg gtcagccagc aagtcctttt
    15541 catgacggat atcgatttat ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa
    15601 attagaacaa ccaacaatcg aagaactttt tgaagacgaa gaggaagaga atgaataaag
    15661 aattatcaag acacgagatt cgggaaatgg cattgcaggc tttatttcca ttagacttca
    15721 atgcagacct aacaaaagaa gatgcgattt tcaacgcaat cgaattagac caccgcgaca
    15781 tgatcaacga agatgaatca gagtttgttc ctgtttatct tgatacgcta gttggcggtg
    15841 tctgtgcaaa aaaagatgaa ttggataaag tgattgaaaa acatttaaaa aacaattggc
    15901 acatcaatcg gatctcaaaa atggatttgg tgatcttgcg tatcgcgatt tttgaaatga
    15961 cttatgtgac agatgtacca gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa
    16021 catttagtga tgaccgttca cgtaaattcg tcaacggtgt tttatccaat gtattgaaag
    16081 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
    16141 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
    16201 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
    16261 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
    16321 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
    16381 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
    16441 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
    16501 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
    16561 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
    16621 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
    16681 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
    16741 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
    16801 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
    16861 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
    16921 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
    16981 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
    17041 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
    17101 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
    17161 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
    17221 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
    17281 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
    17341 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
    17401 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
    17461 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
    17521 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
    17581 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
    17641 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
    17701 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
    17761 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
    17821 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
    17881 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
    17941 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
    18001 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
    18061 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
    18121 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
    18181 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
    18241 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
    18301 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
    18361 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
    18421 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
    18481 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
    18541 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
    18601 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
    18661 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
    18721 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
    18781 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
    18841 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
    18901 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
    18961 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
    19021 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
    19081 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
    19141 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
    19201 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
    19261 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
    19321 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
    19381 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
    19441 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
    19501 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
    19561 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
    19621 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
    19681 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
    19741 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
    19801 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
    19861 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
    19921 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
    19981 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
    20041 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
    20101 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
    20161 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
    20221 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
    20281 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
    20341 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
    20401 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
    20461 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
    20521 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
    20581 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
    20641 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
    20701 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
    20761 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
    20821 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
    20881 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
    20941 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
    21001 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
    21061 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
    21121 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
    21181 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
    21241 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
    21301 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
    21361 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
    21421 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
    21481 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
    21541 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
    21601 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
    21661 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
    21721 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
    21781 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
    21841 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
    21901 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
    21961 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
    22021 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
    22081 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
    22141 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
    22201 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
    22261 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
    22321 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
    22381 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
    22441 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
    22501 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
    22561 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
    22621 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
    22681 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
    22741 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
    22801 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
    22861 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
    22921 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
    22981 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
    23041 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
    23101 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
    23161 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
    23221 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
    23281 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
    23341 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
    23401 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
    23461 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
    23521 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
    23581 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
    23641 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
    23701 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
    23761 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
    23821 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
    23881 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
    23941 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
    24001 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
    24061 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
    24121 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
    24181 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
    24241 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
    24301 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
    24361 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
    24421 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
    24481 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
    24541 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
    24601 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
    24661 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
    24721 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
    24781 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
    24841 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
    24901 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
    24961 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
    25021 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
    25081 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
    25141 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
    25201 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
    25261 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
    25321 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
    25381 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
    25441 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
    25501 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
    25561 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
    25621 ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg
    25681 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
    25741 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
    25801 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
    25861 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
    25921 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
    25981 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
    26041 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    26101 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    26161 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    26221 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    26281 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    26341 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    26401 attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc
    26461 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    26521 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    26581 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    26641 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    26701 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    26761 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    26821 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    26881 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    26941 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    27001 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    27061 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    27121 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    27181 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    27241 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    27301 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    27361 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    27421 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    27481 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    27541 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    27601 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    27661 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    27721 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    27781 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    27841 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    27901 gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg
    27961 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    28021 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    28081 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    28141 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    28201 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    28261 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    28321 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    28381 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    28441 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    28501 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    28561 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    28621 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    28681 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    28741 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    28801 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    28861 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    28921 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    28981 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    29041 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    29101 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    29161 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    29221 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    29281 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    29341 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    29401 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    29461 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    29521 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    29581 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    29641 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    29701 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    29761 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    29821 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    29881 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    29941 cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    30001 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    30061 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    30121 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    30181 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    30241 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    30301 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    30361 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    30421 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    30481 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    30541 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    30601 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    30661 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    30721 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    30781 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    30841 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    30901 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    30961 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    31021 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    31081 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    31141 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    31201 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    31261 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    31321 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    31381 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    31441 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    31501 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    31561 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    31621 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    31681 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    31741 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    31801 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    31861 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    31921 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    31981 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    32041 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    32101 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    32161 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    32221 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    32281 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    32341 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    32401 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    32461 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    32521 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    32581 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    32641 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    32701 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    32761 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    32821 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    32881 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    32941 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    33001 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    33061 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    33121 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    33181 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    33241 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    33301 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    33361 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    33421 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    33481 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    33541 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    33601 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    33661 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    33721 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    33781 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    33841 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    33901 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    33961 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    34021 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    34081 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    34141 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    34201 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    34261 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    34321 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    34381 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    34441 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    34501 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    34561 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    34621 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    34681 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    34741 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    34801 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    34861 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    34921 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    34981 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    35041 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    35101 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    35161 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    35221 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    35281 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    35341 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    35401 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    35461 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    35521 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    35581 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    35641 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    35701 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    35761 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    35821 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    35881 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    35941 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    36001 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    36061 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    36121 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    36181 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    36241 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    36301 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    36361 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    36421 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    36481 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    36541 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    36601 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    36661 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    36721 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    36781 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    36841 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    36901 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    36961 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    37021 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    37081 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    37141 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    37201 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    37261 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    37321 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    37381 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    37441 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    37501 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    37561 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    37621 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    37681 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    37741 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    37801 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    37861 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    37921 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    37981 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    38041 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    38101 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    38161 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    38221 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    38281 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    38341 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    38401 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    38461 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    38521 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    38581 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    38641 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    38701 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    38761 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    38821 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    38881 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    38941 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    39001 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    39061 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    39121 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    39181 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    39241 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    39301 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    39361 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    39421 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    39481 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    39541 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    39601 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    39661 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    39721 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    39781 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    39841 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    39901 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    39961 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    40021 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    40081 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    40141 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    40201 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    40261 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    40321 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    40381 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    40441 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    40501 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    40561 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    40621 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    40681 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    40741 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    40801 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    40861 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    40921 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    40981 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    41041 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    41101 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    41161 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    41221 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    41281 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    41341 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    41401 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    41461 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    41521 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    41581 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    41641 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    41701 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    41761 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    41821 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    41881 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    41941 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    42001 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    42061 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    42121 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    42181 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    42241 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    42301 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    42361 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    42421 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    42481 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    42541 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    42601 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    42661 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    42721 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    42781 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    42841 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    42901 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    42961 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    43021 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    43081 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    43141 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    43201 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    43261 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    43321 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    43381 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    43441 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    43501 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    43561 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    43621 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    43681 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    43741 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    43801 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    43861 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    43921 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    43981 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    44041 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    44101 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    44161 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    44221 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    44281 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    44341 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    44401 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    44461 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    44521 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    44581 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    44641 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    44701 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    44761 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    44821 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    44881 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    44941 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    45001 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    45061 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    45121 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    45181 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    45241 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    45301 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    45361 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    45421 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    45481 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    45541 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    45601 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    45661 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    45721 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    45781 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    45841 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    45901 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    45961 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    46021 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    46081 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    46141 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    46201 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    46261 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    46321 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    46381 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    46441 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    46501 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    46561 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    46621 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    46681 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    46741 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    46801 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    46861 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    46921 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    46981 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    47041 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    47101 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    47161 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    47221 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    47281 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    47341 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    47401 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    47461 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    47521 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    47581 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    47641 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    47701 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    47761 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    47821 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    47881 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    47941 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    48001 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    48061 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    48121 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    48181 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    48241 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    48301 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    48361 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    48421 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    48481 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    48541 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    48601 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    48661 ttgtgcggat tatgatcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    48721 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    48781 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    48841 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    48901 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    48961 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    49021 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    49081 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    49141 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    49201 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    49261 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    49321 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    49381 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    49441 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    49501 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    49561 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    49621 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    49681 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    49741 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    49801 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    49861 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    49921 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    49981 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    50041 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    50101 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    50161 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    50221 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    50281 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    50341 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    50401 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    50461 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    50521 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    50581 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    50641 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    50701 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    50761 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    50821 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    50881 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    50941 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    51001 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    51061 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    51121 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    51181 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    51241 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    51301 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    51361 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    51421 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    51481 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    51541 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    51601 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    51661 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    51721 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt
    51781 gaacggattt tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg
    51841 aagtaaaaaa tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca
    51901 aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg catactgccc
    51961 gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa
    52021 cagtttaaca gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg
    52081 aatgtcatca tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg
    52141 atctttgcct tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct
    52201 ggggggatcg ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca
    52261 gcagcattag atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt
    52321 ggtgcaacga ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat
    52381 catttgaatg gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt
    52441 ttttgtaaat tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca
    52501 gtatctaaag catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt
    52561 atcaattata tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa
    52621 tatattgcag aaccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca
    52681 gtatttgttc aagtattctg gttctttggt attcatggat caaatgtatt ggcaccagtg
    52741 ctagagggaa tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat
    52801 aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg
    52861 tgggtccgcg gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc
    52921 ttactgatag ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta
    52981 tccattgggc ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta
    53041 ttgaatgcaa tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga
    53101 tggacagcta cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta
    53161 ccgccgttct tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca
    53221 ttagtatgta tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa
    53281 atggaccctg ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt
    53341 ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat
    53401 gcaacaagat gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac
    53461 atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt
    53521 tgcggctaaa aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt
    53581 tcaatcaaca gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa
    53641 cggtttattt ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc
    53701 gcaaaggtat gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta
    53761 tttcttcatg aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc
    53821 acgaaaaaat taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt
    53881 ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag
    53941 tacgtcaaac aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa
    54001 aaccagtctt ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa
    54061 atcaaaccat tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat
    54121 ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg
    54181 agacgtttca aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg
    54241 tactatttag ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat
    54301 ctttcctgcg aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag
    54361 taagagagct aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta
    54421 atggggcaga tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga
    54481 attacggatt ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc
    54541 cagtgaattt ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc
    54601 cagaagcaag gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc
    54661 gtgatcgtaa agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct
    54721 atggctatga acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt
    54781 ttatcaaaaa aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa
    54841 tcagaagaat ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact
    54901 ggtggtatac gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg
    54961 ataatgagga agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg
    55021 cggagtgggg atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc
    55081 acaatggtgc ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt
    55141 atctattaga aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg
    55201 tagatatttc tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc
    55261 tacaaataga agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg
    55321 aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa
    55381 gtatccaaca tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc
    55441 aagaaagaat acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc
    55501 aacagccaat tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca
    55561 ccaccctttt gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt
    55621 cgtaaatttg aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat
    55681 tgcttctttc gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac
    55741 tttttctttt ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc
    55801 tagtttatca tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt
    55861 ttagcagata agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt
    55921 cctgtagctc agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc
    55981 agtcaggaac gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt
    56041 gtgccaaaaa tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga
    56101 ttttttcttt atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag
    56161 tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa
    56221 gtaaaataga tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta
    56281 attcttttaa ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt
    56341 ttaagttgaa aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt
    56401 tttgattgat tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc
    56461 tatttgaagt ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat
    56521 taatagtgat gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagtccag
    56581 caaatcgcat tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag
    56641 aaaggttttc ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat
    56701 cttcttttgg cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg
    56761 gaattaatcc ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa
    56821 taacggtaga ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc
    56881 tatctaaatc agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt
    56941 cgtattttct ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct
    57001 caaagtaatc tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat
    57061 attctaattc gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta
    57121 tcttcgtgta ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac
    57181 gtttgactaa tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata
    57241 aagaagccca ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa
    57301 ggatgtgcta tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga
    57361 ggtgtttatt atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt
    57421 atttgttgat tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt
    57481 catattagaa agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat
    57541 attttgtagc ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt
    57601 tctaacagac caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag
    57661 ccagacatgc tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac
    57721 aattgaatct caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc
    57781 catgaagcgt tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt
    57841 ttattttgtg cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt
    57901 tggtccttgt tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat
    57961 gattcgttta aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac
    58021 ccaaaataaa gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca
    58081 actattaatt caagatattc ttttttggtt agtttttcat tgctattatc catagttacc
    58141 tcctattttg tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg
    58201 ttttattatt gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac
    58261 tttactatta atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact
    58321 aatttctcct gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc
    58381 cttcgcaaaa attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc
    58441 atcgtactct ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc
    58501 ttcaaaagta tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc
    58561 tggagtaata gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa
    58621 aaattcttca aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc
    58681 agatgatgag ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa
    58741 acctacggta gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt
    58801 ttttttgata aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt
    58861 aaaaccaaat ataccgtcca ttaatagatc ctctttctct tttgatattg aatattataa
    58921 accaagcaat tcttttttct taaaatcgaa ttcttcttga ttaataatcc cttcatctaa
    58981 caactcttta tactttttaa tttcatctgc acttgaaatt tgtgtttctt tggaattatt
    59041 atctaatata gattgttgct tctctatata tttttttaaa ttttccattt cattccaata
    59101 tttttttgta aacataacgg tgttttcatc tttcgtggcc gctaatatac ctccgcgact
    59161 ttcgtttccg cctagcaagg taaattgtat ataaccatta ctcatacctg gtttttttaa
    59221 ttgtacggct gatatatttt taaaggggat agttttttct cctttaatac cttgattaac
    59281 taaatttata aatccttttc taataattga aatagaatca tcagttactt taatcaaggt
    59341 ttttcctgga gattttatta aaatctcttt ttccattttt tcttcctcat ttctgtgata
    59401 tgattttttt tgtagaagct catatatgag gaaagagccc gtgttgcagc acgggctttt
    59461 ttaatgtttt gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct
    59521 tgcgtaagaa tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag
    59581 cttattctga atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt
    59641 cagttcgttc tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt
    59701 ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa actgaaaatt caaaatgaga
    59761 ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct tctgcagttt caccgtatag
    59821 attttcattc aaaaaaatga ttctgggttt tggatagtag aaaccaggct ctttcatctc
    59881 catataaact actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca
    59941 aaccatcact ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc
    60001 attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtc ttctgataaa
    60061 tccacatgtt taggggtttc tctttcttct ctacctagaa ggtagtctac agagacatcg
    60121 aaatagtcag caatccgact taattcatct gaattaggag tattattttt ccactttgct
    60181 agataaccgt tagaatatcc aaaattaatt tctaactgcc gtatagatat ctttttcctc
    60241 ttcgccaagt cttttattat ttcataggta ttcatcgata aaacaacctt tctaaatgtt
    60301 tacaaaaaaa gtttagaaaa atacgcagaa ataatttgac taattctgag taatgagcta
    60361 tactatatct tgtaaacaga tttaacaact aaaaagacaa caaaaaacac tattgatatg
    60421 taaatgcaga ccgccaagaa agctttaaaa tcaatgtttt tatgtcttat ttaattatgc
    60481 tcttagtata gagtattact cagatactgt caattgaatt tagaaaaaag ttgttaaatt
    60541 tgtttacgaa tataaaagaa aggaatgaga aatatgaaca caccgcaaat ttttaatttc
    60601 gaacaaaacg aagttcggac aattttagta aatgatgaac catattttgt aggcaaagac
    60661 gttgcgagtg ttttgggtta ttcaaacact aaagatgctt tgtcgcggca tgtagatttg
    60721 gaagataaga tggggtcgcg aatcacgacc tcaggtcaat caagagagat gacaatcatc
    60781 aacgaatctg gtttgtacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt
    60841 aaacgttggg taacaagtga agtgttgcca gcaattagaa aacatggagg ttatctaact
    60901 ccagaaaaag tagaagaagc tttgcttaat ccagatacaa tcattcaatt agcaactcaa
    60961 ctaaaagaag aaagaactgg aagattaatc gcagaacaaa agattgcaga gtacgaacct
    61021 aaaatctcct atttagatag catattatct tctacagatt cagtaacaat tagtcagatt
    61081 gcagcagatt atgggatgtc tccacaacag atgaataaat tacttcataa actaggtatt
    61141 cagaaaaaag tcggtaacca atggttattg tgcaaaaaac acatgaacca aggatacaca
    61201 aaatctcata caactgagat cccgaaagcc gatggtggca ctaaaattgt aatgaatacc
    61261 aaatggacac agaaagggcg tctatttatc tacgaattac taaaaaaaga aggatattac
    61321 cctcaaatgg atttagagga aattggttag aaaggagttt tagtatgact gacattgcag
    61381 aaatcactca acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa
    61441 cttacaccat gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa
    61501 acggtaaaaa gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg
    61561 aattgtaaag ggaggaacag ctaatgcaat atctagaagc aaaaatccca attccagaag
    61621 gctatgtaat tatctcccaa gtggattatg aggagttaaa aaaagctgat gatactggta
    61681 gatggatgac gttgccagaa gtgctagaac ggattaacag aaaatatgat tggtttactt
    61741 ctagagtttt aaagaaccca agatatagaa atattatcga tatcgagaaa aacaaaaatg
    61801 gatttgtcta ttatccagtt gaaggaagag acacatatct atttttaaga agtaaaacac
    61861 ttgaattttt agaaacaaat ttttcggaaa tcttaaggag gcaagcggat gggaaaattt
    61921 aatagagcgc tagtgttcag cgcaccgcta atcatctacg ctttaggact ttggggaagc
    61981 aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa
    62041 gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa
    62101 aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta atttctctat
    62161 tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa
    62221 tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc
    62281 aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat
    62341 tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca
    62401 tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga
    62461 atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag
    62521 ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa
    62581 tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa
    62641 caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag
    62701 taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga
    62761 aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg
    62821 gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct
    62881 tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct
    62941 acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata
    63001 ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta
    63061 aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa
    63121 ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt
    63181 atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
    63241 caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg
    63301 aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga
    63361 gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct
    63421 aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa
    63481 aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac
    63541 acatcataag gagattttag catatggaaa aagaactttc cactctagat caatatttga
    63601 ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa
    63661 atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta
    63721 tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt
    63781 acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg
    63841 atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg
    63901 agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt
    63961 ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa
    64021 aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct
    64081 ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg
    64141 acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccgaatt atttcctgtt
    64201 ttagattatc gaaaccaacc agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg
    64261 aagcgatgct ttgtgaaagc attggctcta cacggactgg gattatatgt atttcaaggg
    64321 gaagatattc caacaccacc tagaatcgat acaaagaaat taaacatgct agagacgatt
    64381 ctagaagctt tcaatgagca gatgggtaaa gatatgacca aaaccttaat cgaatatgtt
    64441 aatgagcaga cagataaatt agggctctta gctgataacg ttgaaactat tgaacagtta
    64501 agctatgagc aatgtgcctt gatggagcga gcaatagcag ctaagagaaa agaattagat
    64561 aagaagtgat atgagtgttt aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt
    64621 tcaaaggaaa agacataagc gcaaccatca atgaggaagt gaacattgat cgactaaaga
    64681 cgatgtatga cggctacgat ggtgatcgag tcattgaaat tcgttttatt gatcctagac
    64741 gtttcaccgt acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata
    64801 caggcatgcc aacggacttc tggaaggaat tcttctactt tcgttttgaa ggtgtcacag
    64861 ggcgcaaaat aagcctgaaa gacgaatcga atacaactgt gagtgatgcc aatgtcttag
    64921 caaatatcat cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga
    64981 ttttacctgc gaatcaagaa tattacttct acaaatgcat cacaaaaaga gtctgctgca
    65041 tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc
    65101 gcaaagaagt tgatcatgca gagtacacat ttgcagcact ctgcagaatc catcacacag
    65161 agaagcacaa aataggtgtg attaatttca aaaataaata tcaaatcaaa gggatcaaat
    65221 taaaccagaa aacaatcaaa aagttaaata ttggagggga aaaatgacag aacatcgaag
    65281 ttattatgcg attataccag ccaacgtaag gtacgacaaa agacttaaac caaatactaa
    65341 gttgttatac ggagagataa cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa
    65401 tgagtacttt gcagatttat atggtgtgaa taaagagacc atatcgcgat gggtaagtga
    65461 tttgattaag tttggatact tgaatcggga aatcatttac aaagagggta ccaatcaaat
    65521 aatcaatagg tacctacgaa ttaatcaata ccctattgac gaaaaacgca atacccctat
    65581 tgacgaaaaa gtcaaagata ataatacatc tattaataat acatttaata atacaaaaga
    65641 atatataaga gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata
    65701 cggagagtat aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaacagaatt
    65761 ccctaatgat taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa
    65821 gacttataaa aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc
    65881 taagaacgca agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc
    65941 aatcgaccaa gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa
    66001 agcaccattc taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata
    66061 gcgaattata aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa
    66121 gaagctggaa ttgaaggaat taagaatgat cgttcgagtt ggagtaaact cagatactat
    66181 ttcgcactct atatcgatgg aacgcaatta agaaatttag catataccaa attactattc
    66241 attgattgtg ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaaacaaga
    66301 tggacctaaa gacatttaca gcacagatcg aattaatgca tcaagaagct ttaagacaaa
    66361 gtgtgtcgta cgaagacaag tggctcaaca catttcatgg cggacgtgag agcgcacttg
    66421 atcaagtact caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag
    66481 cgaattatcg aattgactta ttcagaagat tggcaggaag ataaaaaaat agttgcagaa
    66541 gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaag
    66601 attgcaatct ggcatggtga tcgaattcta gtaacgggta ccgctaaaca gttatctgaa
    66661 attactggtc tgagcaaaaa tattatttgg gatagagcta ggagcttatg gattgattca
    66721 aaaggaagac agtttaggta tgtggaggag aaataatgga cgaactaatc gcgaaagtag
    66781 agcagtgggc aaaagataag ggattggatc aagcggattt cagcaagcaa atgttaaaaa
    66841 cggtcgaaga aatcggggaa gttgccgctt ctctagctag aaaagatgaa catggtttaa
    66901 gagacggaat tggcgatgta gtagtaacct tgatcatctt ggcaatgcaa aatgatatgg
    66961 atttatacga gtgcttggat tttgcttatg acgaaatcaa aggtcggact ggaaagatgg
    67021 tcaatggtgt tttcgttaag tccagtgacc tgaaataatt ccattaatgg aagaaataac
    67081 tgaataggag gatagacatg attgatgcaa aacaagtgca aaaacagaaa gatggaatgt
    67141 tgatgtttga agcatatgtt ctaccatttc ttaatcaatt tgaagttttg gaatgctctg
    67201 ccagcggaga agaattggag tatgttgtaa ttcgtgaaac caaagaaaac gttcagaagc
    67261 taaacgaatt tctttgcact attaactgtt gggatatgat agctccagga tttttatgtc
    67321 cagctatggg agaatttttg gaatattgtc gtcttgaaga tgcaggcact ttggatttag
    67381 cttatctggt atacaactac ctaaacatca acacagatca tttgtggttt ggcactgccg
    67441 aaagaaaatg ggtagtccgc taaccaccaa tatcaatgaa aaaaggcggt ttcctgaaag
    67501 tggaattgcc gccattgctg taaggttctt tacgcgactc aaggtcacag aaatataaat
    67561 gcaaatctct tttgaagtga tccaaagctc tcggaaaatg gaagtttttt gtttcaaaga
    67621 ataaatgaga tttgcgtcaa gaacctgaga gttatttcca aaaaagcttc caaagaaccg
    67681 aatgagctaa ggacatcatg tgtaaacaca taatatagct ctcctttcac ggctaatcat
    67741 aacatgaaaa atattcgatg tcagtaggaa aaaaggtgaa ttatgaaaag aatattaaat
    67801 tatcccggca gtaacagatt ctgcaattaa actccgaagc tgatgtatca gtatcaactg
    67861 tagaatatta tgagcgtgga gaaagtcaag cagtcacaaa tgtaaaaatc gataacgtca
    67921 acggtcttat ctgtttagat gaaggcgata gtatgaatga aattgctgca gaccatgaat
    67981 tatcagaaac tgctttaatt gatcaaatcg aattagctag acaatacttt gttccatatg
    68041 cagatacctg gtccaagcat aagaatgaca tcatttttcc agaggaacaa tgagagcaac
    68101 tgatccagta attatccttg aggaagccaa atttatttgg actcacgaag agatagagca
    68161 agcacgcttg ctcttttctc aaggagttaa gccgagcaaa gtagctgaaa taatgggtca
    68221 aaagattctt gatgtcggat tgcttttgct ccatctagca gaaaaaattt gatttgaggt
    68281 gaaaatgatg attcaacttg caggcatcca aacagggaaa atttattttt ctggagaaag
    68341 caaaagcgaa gccaaccaat ggctattgaa aacttacacg aacaataaga agctaaggaa
    68401 aaaatatcct gatttgttgc taaaggatga tcagattatg ccggaaccga tgattttgac
    68461 ccgtaaggag ggaagttgaa actaaaaaac cagccgtgtg ggctggagtt gctatttctt
    68521 tttaataatg agtttttcta aatttaattc attctcacga gtttgcgtcc aataatcaga
    68581 ataatatt
//
LOCUS       DAZKPO010000005        59540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000005 DAZKPO010000000
VERSION     DAZKPO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.005"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..528)
                     /locus_tag="ACG8NZ_000318"
     CDS             complement(142..528)
                     /locus_tag="ACG8NZ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0538025.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(525..1046)
                     /locus_tag="ACG8NZ_000319"
     CDS             complement(525..1046)
                     /locus_tag="ACG8NZ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0538026.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(1048..2082)
                     /gene="sppA"
                     /locus_tag="ACG8NZ_000320"
     CDS             complement(1048..2082)
                     /gene="sppA"
                     /locus_tag="ACG8NZ_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0538027.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(2115..3530)
                     /locus_tag="ACG8NZ_000321"
     CDS             complement(2115..3530)
                     /locus_tag="ACG8NZ_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0538028.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3499..5652)
                     /locus_tag="ACG8NZ_000322"
     CDS             complement(3499..5652)
                     /locus_tag="ACG8NZ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0538029.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5910..6146
                     /locus_tag="ACG8NZ_000323"
     CDS             5910..6146
                     /locus_tag="ACG8NZ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0538030.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            6165..6380
                     /locus_tag="ACG8NZ_000324"
     CDS             6165..6380
                     /locus_tag="ACG8NZ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0538031.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(7083..7835)
                     /locus_tag="ACG8NZ_000325"
     CDS             complement(7083..7835)
                     /locus_tag="ACG8NZ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0538032.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(7850..9097)
                     /locus_tag="ACG8NZ_000326"
     CDS             complement(7850..9097)
                     /locus_tag="ACG8NZ_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0538033.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(9173..9319)
                     /locus_tag="ACG8NZ_000327"
     CDS             complement(9173..9319)
                     /locus_tag="ACG8NZ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0538034.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(9423..9734)
                     /locus_tag="ACG8NZ_000328"
     CDS             complement(9423..9734)
                     /locus_tag="ACG8NZ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0538035.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(9736..9933)
                     /locus_tag="ACG8NZ_000329"
     CDS             complement(9736..9933)
                     /locus_tag="ACG8NZ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0538036.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(10327..12054)
                     /locus_tag="ACG8NZ_000330"
     CDS             complement(10327..12054)
                     /locus_tag="ACG8NZ_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0538037.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(12047..13240)
                     /locus_tag="ACG8NZ_000331"
     CDS             complement(12047..13240)
                     /locus_tag="ACG8NZ_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0538038.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(13427..14071)
                     /locus_tag="ACG8NZ_000332"
     CDS             complement(13427..14071)
                     /locus_tag="ACG8NZ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0538039.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(14165..14299)
                     /locus_tag="ACG8NZ_000333"
     CDS             complement(14165..14299)
                     /locus_tag="ACG8NZ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0538040.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(14301..16433)
                     /gene="feoB"
                     /locus_tag="ACG8NZ_000334"
     CDS             complement(14301..16433)
                     /gene="feoB"
                     /locus_tag="ACG8NZ_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0538041.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(16430..16903)
                     /locus_tag="ACG8NZ_000335"
     CDS             complement(16430..16903)
                     /locus_tag="ACG8NZ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0538042.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            17304..17528
                     /gene="nrdH"
                     /locus_tag="ACG8NZ_000336"
     CDS             17304..17528
                     /gene="nrdH"
                     /locus_tag="ACG8NZ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0538043.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            17687..19846
                     /gene="nrdE"
                     /locus_tag="ACG8NZ_000337"
     CDS             17687..19846
                     /gene="nrdE"
                     /locus_tag="ACG8NZ_000337"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0538044.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            19896..20861
                     /gene="nrdF"
                     /locus_tag="ACG8NZ_000338"
     CDS             19896..20861
                     /gene="nrdF"
                     /locus_tag="ACG8NZ_000338"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0538045.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(20950..22089)
                     /locus_tag="ACG8NZ_000339"
     CDS             complement(20950..22089)
                     /locus_tag="ACG8NZ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0538046.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(22398..23111)
                     /locus_tag="ACG8NZ_000340"
     CDS             complement(22398..23111)
                     /locus_tag="ACG8NZ_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0538047.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(23365..24066)
                     /gene="nagB"
                     /locus_tag="ACG8NZ_000341"
     CDS             complement(23365..24066)
                     /gene="nagB"
                     /locus_tag="ACG8NZ_000341"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0538048.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            24300..25832
                     /locus_tag="ACG8NZ_000342"
     CDS             24300..25832
                     /locus_tag="ACG8NZ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0538049.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(26023..27225)
                     /locus_tag="ACG8NZ_000343"
     CDS             complement(26023..27225)
                     /locus_tag="ACG8NZ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0538050.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(27307..27996)
                     /locus_tag="ACG8NZ_000344"
     CDS             complement(27307..27996)
                     /locus_tag="ACG8NZ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0538051.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(28102..28470)
                     /gene="rplL"
                     /locus_tag="ACG8NZ_000345"
     CDS             complement(28102..28470)
                     /gene="rplL"
                     /locus_tag="ACG8NZ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0538052.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(28523..29026)
                     /gene="rplJ"
                     /locus_tag="ACG8NZ_000346"
     CDS             complement(28523..29026)
                     /gene="rplJ"
                     /locus_tag="ACG8NZ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0538053.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(29230..29357)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(29436..30125)
                     /gene="rplA"
                     /locus_tag="ACG8NZ_000347"
     CDS             complement(29436..30125)
                     /gene="rplA"
                     /locus_tag="ACG8NZ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0538054.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(30229..30651)
                     /gene="rplK"
                     /locus_tag="ACG8NZ_000348"
     CDS             complement(30229..30651)
                     /gene="rplK"
                     /locus_tag="ACG8NZ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0538055.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            30994..31662
                     /gene="sdaAB"
                     /locus_tag="ACG8NZ_000349"
     CDS             30994..31662
                     /gene="sdaAB"
                     /locus_tag="ACG8NZ_000349"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0538056.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            31675..32547
                     /gene="sdaAA"
                     /locus_tag="ACG8NZ_000350"
     CDS             31675..32547
                     /gene="sdaAA"
                     /locus_tag="ACG8NZ_000350"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0538057.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(32638..33738)
                     /locus_tag="ACG8NZ_000351"
                     /pseudo
     CDS             complement(32638..33738)
                     /locus_tag="ACG8NZ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(33830..34717)
                     /locus_tag="ACG8NZ_000352"
     CDS             complement(33830..34717)
                     /locus_tag="ACG8NZ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0538058.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(34794..35339)
                     /gene="nusG"
                     /locus_tag="ACG8NZ_000353"
     CDS             complement(34794..35339)
                     /gene="nusG"
                     /locus_tag="ACG8NZ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0538059.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(35440..35610)
                     /gene="secE"
                     /locus_tag="ACG8NZ_000354"
     CDS             complement(35440..35610)
                     /gene="secE"
                     /locus_tag="ACG8NZ_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0538060.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(35634..35786)
                     /gene="rpmG"
                     /locus_tag="ACG8NZ_000355"
     CDS             complement(35634..35786)
                     /gene="rpmG"
                     /locus_tag="ACG8NZ_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0538061.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            35948..36592
                     /gene="cbpA"
                     /locus_tag="ACG8NZ_000356"
     CDS             35948..36592
                     /gene="cbpA"
                     /locus_tag="ACG8NZ_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0538062.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(36648..37151)
                     /locus_tag="ACG8NZ_000357"
     CDS             complement(36648..37151)
                     /locus_tag="ACG8NZ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0538063.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(37164..37210)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            37748..38713
                     /locus_tag="ACG8NZ_000358"
     CDS             37748..38713
                     /locus_tag="ACG8NZ_000358"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0538064.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(38812..39984)
                     /locus_tag="ACG8NZ_000359"
     CDS             complement(38812..39984)
                     /locus_tag="ACG8NZ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538065.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(39971..41116)
                     /locus_tag="ACG8NZ_000360"
     CDS             complement(39971..41116)
                     /locus_tag="ACG8NZ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538066.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(41130..42054)
                     /locus_tag="ACG8NZ_000361"
                     /pseudo
     CDS             complement(41130..42054)
                     /locus_tag="ACG8NZ_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            42171..43250
                     /locus_tag="ACG8NZ_000362"
     CDS             42171..43250
                     /locus_tag="ACG8NZ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0538067.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            43247..43875
                     /locus_tag="ACG8NZ_000363"
                     /pseudo
     CDS             43247..43875
                     /locus_tag="ACG8NZ_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(43985..44212)
                     /locus_tag="ACG8NZ_000364"
     CDS             complement(43985..44212)
                     /locus_tag="ACG8NZ_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0538068.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(44315..46903)
                     /locus_tag="ACG8NZ_000365"
     CDS             complement(44315..46903)
                     /locus_tag="ACG8NZ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0538069.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(46923..47582)
                     /locus_tag="ACG8NZ_000366"
     CDS             complement(46923..47582)
                     /locus_tag="ACG8NZ_000366"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0538070.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(47631..48134)
                     /gene="trmL"
                     /locus_tag="ACG8NZ_000367"
     CDS             complement(47631..48134)
                     /gene="trmL"
                     /locus_tag="ACG8NZ_000367"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0538071.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(48355..49221)
                     /locus_tag="ACG8NZ_000368"
     CDS             complement(48355..49221)
                     /locus_tag="ACG8NZ_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0538072.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            49330..49971
                     /locus_tag="ACG8NZ_000369"
     CDS             49330..49971
                     /locus_tag="ACG8NZ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0538073.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(50033..51394)
                     /gene="mgtE"
                     /locus_tag="ACG8NZ_000370"
     CDS             complement(50033..51394)
                     /gene="mgtE"
                     /locus_tag="ACG8NZ_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0538074.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(51412..52311)
                     /locus_tag="ACG8NZ_000371"
     CDS             complement(51412..52311)
                     /locus_tag="ACG8NZ_000371"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0538075.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(52313..53110)
                     /locus_tag="ACG8NZ_000372"
     CDS             complement(52313..53110)
                     /locus_tag="ACG8NZ_000372"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0538076.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(53088..53792)
                     /locus_tag="ACG8NZ_000373"
     CDS             complement(53088..53792)
                     /locus_tag="ACG8NZ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0538077.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            53923..54499
                     /locus_tag="ACG8NZ_000374"
                     /pseudo
     CDS             53923..54499
                     /locus_tag="ACG8NZ_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            54617..55294
                     /locus_tag="ACG8NZ_000375"
     CDS             54617..55294
                     /locus_tag="ACG8NZ_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0538078.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(55373..57181)
                     /gene="pepF"
                     /locus_tag="ACG8NZ_000376"
     CDS             complement(55373..57181)
                     /gene="pepF"
                     /locus_tag="ACG8NZ_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0538079.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(57357..58355)
                     /locus_tag="ACG8NZ_000377"
     CDS             complement(57357..58355)
                     /locus_tag="ACG8NZ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0538080.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(58581..59234)
                     /locus_tag="ACG8NZ_000378"
     CDS             complement(58581..59234)
                     /locus_tag="ACG8NZ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0538081.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        18092 a        12172 c        10096 g        19180 t
ORIGIN      
        1 ttttttcgaa aaaattagaa taaataaaag acacgtagtt tttttatcta catgtcttcc
       61 atcgactaca agttatcatt tctcaaaacg tatttagctt ttaattgatt aatcgtttca
      121 cgttgtctac acattgccta attaagattt tcttattaga agcatcaatc ccgcaataat
      181 acctaaaact ccaggaatta cccacaataa tgttaaaaga aaaaatggaa taatagataa
      241 aatgagtaaa ctaataccat agaccactgt tttttgattt tttcttacta aaaaacctat
      301 tatgaaaatc attatcgaaa aaactagggt aattcctcca aataaaacaa cattcccgct
      361 tagattgact aacatatcta tatccttact ggttagttct gtagtattta aagtagttga
      421 atcaatagaa gatagaaaca taccacccat caatgctaaa agactacata aaatccctgt
      481 tattccacta attaaaatca aaataaactc tgtttttctc ttattcattt aattctctcc
      541 agattaagtg ttttagaatt agaaattttt tgaccatagt taatagcttc taatgaacga
      601 ataacaacca ctgacgtgtt agaaaaataa tccccaatat gttgtttctt gggcgtgaaa
      661 gcagccacca aatacccaat ttgaaataca aaatttttta atataaacct acatatacac
      721 tcacgaataa agatagtttg ccaacttagc tctttttcct caaagctcac tactcttatc
      781 ccaaaaatca ttttcccaat tgtttgtcct gaattcagct tagtcaaaag tacaaaatag
      841 gttaaataaa tagctaatga cgctatacca taaaaagtaa taatggattt gtcagattcc
      901 agtcccatga ttttgtaaaa gagacctata gtagcatggg tgatgatgct aatacaaatt
      961 aaatcaataa gaaaagcaaa aaatcgtaac caaaatcccg caaatacata attaggcaaa
     1021 gtttcaatgc cactcttctt attcatttta tagccctcca tagtaataca ttggttttgg
     1081 agtatcagaa aatccaagtt tttcaaaaac tgaaattaca cgatctgatg tagttgcctt
     1141 tagcccttgc cattcagcaa tcttattacc taaccaagaa gaagcaaagc cagttgtgtc
     1201 atttttatat tctataaccg tcgcatcagt taatttcttc tcttttttta gggaatctaa
     1261 tgcatcttca gagtaaccaa tttgatccac caacccattt tctactgctt ggttaccatc
     1321 ataaattctt ccatccgcaa gttctttcac tgcattagta tccatatttc ttccttttgc
     1381 tacaacattg acaaaacgat cataagcact catcacaaat tcctgtataa ctttattctc
     1441 ttcctctgaa ggtttgtgtt gaggtgacat catatctttt aatgcaccac ttttatacgt
     1501 cgcatcagtt acgccaagtt tttctaataa gccagaataa tttaaaccag aaataatgac
     1561 accaattgat ccagttgttg tttcttccgt agcaaatatt ttatctgaac cagcagaaat
     1621 gtagtaccct ccacttgccg ctgtattttt aaatgcagta tagataggaa tatctagctt
     1681 tttaattttt gccatttctt tagcaatctc agcactttca tatattcctc ccccaggtga
     1741 atttacttct aataacactc ccttaaccgc tttgtcttct tgaattttct ttagctgtgt
     1801 taaaaaattc tgatggttat attgttccct actaaaaaga ttgctttcac ctgtatctgc
     1861 aataactcca ttgacggata gtttgacaat ctttttattt gatgcgcctt cttctaacgt
     1921 ttcttctgtg atttcgttag atccgtaaaa aatcttattt attcctgtta gggttgtatt
     1981 tgatgcttcc tctttatctt ttcctggaat tagcgcgacg ataatggata gtaacaataa
     2041 tccgcacgct actcccacag caatccatct tcttttattc atataaactc ctcctatatt
     2101 cttagtaaaa ataattattt tttatttaga aacacatcga caatttgttg ccaatatgtt
     2161 ttttctccta caattaaaga caactctcca gttaatccat aacgaaacgg aaacgattga
     2221 gttgttttta acgttccttt cactgcataa aaaacacctt tctcagtggc agtagaattt
     2281 tctgaaattt catctaaagt tccgtcgatc gtaatcggag ccccaccttt ttgatccaat
     2341 ttaaaatgaa ccttcatccc ttctttaata taggtacttt cattcgcagg taaaagtgct
     2401 gtaaatttca tttttgattt ttcgtggatc ggataaattt ctgcaagcac tgttcctttg
     2461 gctagttctg cttgtccctt tgtttcttca tttaaatgaa ccgttccaga tacaggagct
     2521 tttagtattc cttttgaaag ttgatcgtcc aaacttttga taactgactg gttcttctct
     2581 tgtttttcat ttagctctgt aactgtttgt tttacagttg ttattgcttg ttctagctct
     2641 tgctttcctt tttccgtttg actaattctg tcattctcag aagtagttgg tttaactaat
     2701 ttagcttttt ctgactgata ctgttctact tcttttttta gttgctcaat ttgttcattt
     2761 atgcttgctg aaattgtcag tttcacttga tttttttgat catcagaaac attatttagt
     2821 tgttcttgcc aattctcata gtttgccatc atttcttttg aaaaatcttt taatgattga
     2881 ttattactcc aagctacctg tacttgttgc cattcttgta atttactatc tgaactctca
     2941 atttgttttt ctagttgtct ttttgtcttt tcgtatactt ctaattgctt ttgatcattt
     3001 aattcacttt gcttcaaagc gtagcggagt gattcttttt ctgacaacat gctctttaat
     3061 tgattactgt agccaaaact gtcatccgtt gaaaacaaat tttttccttt acttatactt
     3121 tcaataaata gctgagccat ctttatttgt tgagtaatac gttggttttc ttgttctatc
     3181 tgagcctttt cattctctat taaagaacaa tccaatctaa ccaacacatc gtccttatga
     3241 acaaattgat tttcttttag gaagttttcc attattttag cttctaatgg cacttgtaac
     3301 ttctctattt tttcggggtt caattgagcc gttgtccgaa taacaacctc ttttttagca
     3361 aaggttaaaa acaatccaat aatacataaa aaaagaatta cagggtataa cacccaacgg
     3421 tagaagctat gatgttgttt agtatagata ctagaatgtt cgaaccaatt attattctgc
     3481 ataaattgtt tccctctcct agatttccca taatttttga tagataccat cggcaaaacg
     3541 aagttcatcg tgcgttcctt tttctaccaa atgcccttca tggagaacta aaatttgatc
     3601 acatgctttt gctattggta aatgatgcgc aataaaaata atcgtttttt cttttaaatt
     3661 gaggagataa ttcataattg catgttctaa caaggtatct aatccactag ttgcttcatc
     3721 taaaattaat atgtccggat tttttaataa agcccgtgct aaggctaatc gttgtcgttg
     3781 ccctcctgaa agattatttc caccttcttc caacaatgtc tcgtaacgta atggctgttg
     3841 attaataaat tcatttagtt ggactatctc acaaacttct attatttgtt cgaaacttgg
     3901 tgggctatct aaaccgaata taagattctc aatgattgtt ccactaaaga aaaatgactc
     3961 ttgaggcaaa tatgtcactc gatcgcgtaa tatctgaaaa ggaatgtcta agtgattaat
     4021 gtttccataa tggatcgatc cttctgatgc ttcataaaaa cgaacaaata actttgcaag
     4081 agttgatttt ccagaaccac taactccaac tatagccgtt ttactgtgcg aaggaattgt
     4141 acatgaaatt tcgttcaacg ttggggcctt catgctatac gagaatgaaa cgttttttac
     4201 agtgatatct tgttgaaata attgttcaga aagcatatat tcaaaagact ggttcgattt
     4261 ttctgaatca atcgcgaaaa tttcatttaa acgattattt gcaacttgag ccgtttgcat
     4321 tttcacttgt aaattaataa tgttttgtaa aggatctgtc aaaaatacta gtaaagcatt
     4381 gtatgtgata agttgaccta gactaattct attatccata acaaaataag aacctagcca
     4441 taaaattaat gcactgctaa ttaattgaat caaatgttta attccttgtt gaatattatc
     4501 aagcgtaacc gtacgaaatg attttttcat taatttaaca aattcatggt ctatacggtt
     4561 atatactttc tcttcaccgc tatatgcttt aattgtttct attcctttaa gactttctat
     4621 gatactagaa tttacagctg caccggctgc catttcttcc ctgttcgatt tttcatagct
     4681 tttcacgaaa gcgaatattg caactacata aaaaggcaaa gagcatagag tgattaaaaa
     4741 caacgtacta ttctgaatag ctaacgtaac cccaacaagc aacaccatac caatatctaa
     4801 aaatattgat aacgtagcac ttgctaaagc atcaataatc ttactggcat caagaaaacg
     4861 agaaataatt tcccctgatt ttctcgtagc aaaaaaattc attggtaaag ttaacacatg
     4921 tttgaaatac tgaagcataa ttgccatact cattcgctgt cctaaaataa taagaagata
     4981 actacgacta tattcaaaca atacccgaaa taaatagaca gcgatcaagc caatggaaat
     5041 tatatttaaa gttgaacgtg cttgattagg gatgaaaaaa tcaagaatac cttgaaaata
     5101 atatgagctt cctatgccaa aaaacgtaat gaaaatcgat gctaaaacga tgtggaatac
     5161 taatcctttt tgtttccaaa ttataggcaa aaaagaactt aatccagcta ctttttcttt
     5221 aataggaata taagtttctc taggcatagg taataaaaga atacctgtcc attctttttg
     5281 aaacgcttca atcgtttttc tattttttcc attagcggga tctgcaatcc atagtacgtt
     5341 tcccttcgtt ttataaatta caacgtagtg catatagctg ttatcaacca atatatgtgc
     5401 aattaatggt aagataagat ctctttcttt ccagatctca ctatctgctt gaatagccat
     5461 acaatcaaaa gacaattttt caaatgtctt tttcaatcca aatgctgaag taccctctaa
     5521 atcagtccca gataggtccc gtaatttatg aatcgggata tcagttttaa aatattttaa
     5581 aatcatcgaa aggcaagcaa caccacaatc tttttcatct tgttgctgca caaaaggaaa
     5641 tgattttatc acttatctaa cacaactcct tctaaaaaat ttttatagaa aaattctcaa
     5701 tatagcttta atacagtgaa aatattgtct cataattcca caaaaacttt gaaaacagtt
     5761 cttgaacact aactttttca ttcctgaatc aaatttattt tggaaatttt actattcaag
     5821 aataaacttg ataagtttca ggaagatttt tactgttaat caaatacgta tgttacagta
     5881 gcaccgtaaa aaatattatt taaagaagcg tgtttaaaca tgcaaaatgt aaaggaacta
     5941 agtattgtgg aaatgcacaa actgttggtg gaggcaataa tgcttgggga aaattggggc
     6001 aagttgtagg tggacttacc acaggggcag taggtggtgc aggtttagga acagcaattt
     6061 gcggaccagc ttgcggtgtt gttggagggt tatatggagc tgtcgctgga ggtgctgcag
     6121 ctggctggga tgctcgaaaa aaataaaaaa ggagaataag tagtatgcat attaaaaaca
     6181 caaaaacaac atttatatta tcttcagaag agttgaaaaa tattcaaggt gggagtgccg
     6241 tgggagtttt aggcactact ttttctgggg caacagcagg agtaaaactt tgttcagccg
     6301 gaggacctta tgcaatagct gcttgtggtg taggtggagc tttattaggt gctggattca
     6361 gcatgtggac cggtgcgtaa acaattaaaa aatatcaaac ctcaagattg gtttgtgtcg
     6421 attacgaaca taggtatgtt ctttatcttt tcaaaagttt ggaatggaca gaacttttta
     6481 ctaaagcttc ttgtgctagt ttttataatt atattaaatt tttatctttt attaaagtca
     6541 gataaaaatt ctttggaaat gtaatggtag agatattttt acaaaaggac ttgcagcgct
     6601 gtagttggtg ctgcaggagg acctatttcg gccgttacag gaggtattgg taatgtactt
     6661 gtcggatgtg cattaaagcc tgttcctgca agttaaattt ataaaaacag gaaagtaatt
     6721 atctcaaatc ctatcaagta caatcatagg attgcacttg acaggacaaa ttttttaact
     6781 aaaaaatgaa aaacactttc taaaaattag tagaatttgc caaatctttc gcatcttcca
     6841 ccatacattt aattttaatt cccctttatg gtacttaata aaaacaagaa aagaactatt
     6901 catttggccc ttttttggtt ccagaccata gaaatattcc tccagtaact aatattgctg
     6961 ttatgcacgt aactaataac agctataaaa attggtacaa ctttaatagg attcagaaaa
     7021 atttgcacta gtgaaggaat ctattttatt agaaaaatgc aatctcccta aagaaaaaac
     7081 cactaaacag tgctttcttt caacttctcc ttaatttttt gtgctttccc taaagtaaag
     7141 tttctggcca actctgaatc aaaatatagt gaacgttttt taaaatctat ttctttaacg
     7201 ttaaagatat ttactaaaca cgctcgacta attcttgtta acataggata tttcttttca
     7261 agctcactaa ttcttccata aaattcgtac tgcccatctt tagtacacaa tgataatcta
     7321 tgtggtaatt ttgaagcttc tacaaaataa atgtcattta tatcaaaagt aaatgtttga
     7381 gaacctatgg aaaaaacaaa tgcttgattc gtaacctgtt ttgttgcatc tatcctttct
     7441 tgcgctaata acaataactc aacaatctcg tttcgatact catctagtgt ttgatctttt
     7501 gttacaaatc ctaacgtttc aactcttctt tttattgtta cgggtaacat ctcatcatga
     7561 gtagttgtaa aaatgatttt tgcttgaaca tcatattttc tgattacttc tgctaattct
     7621 ataccgttaa cttcatgatt taaatcaata tctaagaaat atattccttg ctttggtcgg
     7681 aattgtttga ggtatttttt aacttctaac ggactttgtg ttttcaatac tatcttaaat
     7741 actttatcgt gaaaaagaat gaaattatca ataattcgtt ctaactgatt taattgtata
     7801 atttgatctt cacataatat tattggataa ctcataaatc ctccttccct caaaacaaca
     7861 aaataacttg agtagtaaat tgatacggat cgttttgata gttcacaaac atattgggat
     7921 tctttttatt gatttcctct atactagata aacctaaccc ttgatgtccc ttttttgttg
     7981 ttattccttt tttcattaga gtatctatag agagtagtat ctctctacaa ctattcgata
     8041 tcgaaatttc tatctgttta tcatctcggt aaatagctag agacagttct ctatcttgac
     8101 tctctataga tgcttcaatt gcattatcga gaataattcc aagcacccta atacagtcaa
     8161 atattggaac aggaacttca ataatagtac ttggacattc gaatttacaa caaatcttat
     8221 tgttatttgc ttggtatagc ttcgctatca acaaactttt caaatattca ttttttatat
     8281 tttttaaata acgatattca aacttattgg ttagataaga attagaatag atctctaatt
     8341 cttttatttc tttttccaat atcgaatcat ggttttgatt agcgatttct tttaaactca
     8401 gcaataaatt tttatagtca tgtctaaatc tggcaaattt ttcttgatct ttttccagtg
     8461 aatcagtata tttttttaga taaaccaatt cttttctttt caattgctgt tcatattgtt
     8521 ctcgttgcct aatagttatt ctaataaata aaaatagaac aacaaacatt tgaatgatta
     8581 aaaatattaa gacaccaaac acaaaatgat caaatacttg atattgatga gcaacataag
     8641 aaacaaataa accaattgta agtaaataaa tcatgaatat agcagtaagt ttagaactgg
     8701 actgttcaac aatcctattt atagctaatt ttctatataa ccaaataaac atcagcaaaa
     8761 acaaagaatc aagcattatc tggaatacta cataaccata cccttttatc ccttcaaatg
     8821 aaaacatata cgtcataacc atggaagtaa taatagatct gaaatcatca ataagcatac
     8881 ataatagtaa tttatttaaa agcactatat tagttttctt cttttccttt aatataaaat
     8941 aacttccgat tataggtata gctgcggaaa catcagtaaa taattcaaca aaaaaagtga
     9001 gtggtatcaa taaacataca aaaagatatt ttcttttgtt aagatcagta ttagtcatat
     9061 aaaataagag aaaaaagaaa aagtttccca cgaacattct taatagataa gcttttgtat
     9121 cgatagtaat caagagatcc cctcctgatt ttaggatatc ataaaaaaaa ttttagttac
     9181 actttttaaa taatgaaaaa acacattcca ctaaatttga tgccggtttt ccttgaggtt
     9241 ttgttccagc acctccttta atttttttaa gctcttgatc tgaacattgt ttagcattta
     9301 gtctattttt ttcttccatt acgattcccc ctacaaacta taaatacttt tatgtcactg
     9361 attcaatata cctatttttc taatcaaaaa attttgagac atatttaata ttataagtat
     9421 cattaaaatt gagatttatc tccataatct gctcgatata aaccatttat tccagcagtt
     9481 cttccaattt cactcaaatt tcttattaat tcctcttgat attcagtgaa tcttatttta
     9541 ttagtataag cagtaaaata tagataattc accaatctat ttattaaagg accgggatct
     9601 aattcatatt tttgtttttc taattttaaa tatacctttt gtaaaacctc aagaatatca
     9661 gaatatttag gatctttact tatagatata tcattatata attcatgaac aatttgctta
     9721 gcattttttt tcattttagc acttccctgg aattgctcca cctaaaaaac cacctataga
     9781 cattcctgca atacaagtag ttgccttggc ccaatcgacc gtacatttat ttttagtgca
     9841 atacactcca tttccataat attttccact atgagtggta cccccataga taagttgtgt
     9901 ctctttaata gacaaaattt ttaaatgttt cataatctaa tcacccctaa attaatgttt
     9961 ttagactatt attctaacat tcattaaatt tcaaaaaaca attccttctt gaaacgtagc
    10021 aaaataattc ctgaataaga atttaatcac ttttaagtat ataaactatg tatattcttt
    10081 aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac
    10141 ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttgg
    10201 acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac
    10261 atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct
    10321 gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc
    10381 tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc
    10441 tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt
    10501 tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta
    10561 tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc
    10621 gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac
    10681 tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc
    10741 agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga
    10801 atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc
    10861 taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca
    10921 agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc
    10981 taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt
    11041 tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg tttctttggc
    11101 agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag caccaatgat
    11161 cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg
    11221 ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc
    11281 gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa
    11341 gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg
    11401 attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg
    11461 acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat
    11521 ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc
    11581 tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat
    11641 tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat
    11701 caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt
    11761 gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa
    11821 tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa
    11881 caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc
    11941 ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt
    12001 gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac
    12061 tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta
    12121 attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat
    12181 cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg
    12241 gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc
    12301 tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa
    12361 cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg
    12421 taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat
    12481 tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga
    12541 ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt
    12601 gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa
    12661 gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta
    12721 ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa
    12781 gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac
    12841 cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga
    12901 agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta
    12961 acttcgatac atcttctcca tcaatataga tactgcccga agttggctca atcagacgat
    13021 tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt
    13081 ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta
    13141 aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac
    13201 caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg
    13261 taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt
    13321 tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta
    13381 aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct
    13441 gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat
    13501 gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat
    13561 tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg
    13621 aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca
    13681 tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca
    13741 gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt
    13801 gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc
    13861 cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg
    13921 tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac
    13981 ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc
    14041 gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc
    14101 ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga
    14161 ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat
    14221 tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac
    14281 ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg
    14341 ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca
    14401 agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca
    14461 aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg
    14521 gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg
    14581 aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt
    14641 tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata
    14701 ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca
    14761 agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca
    14821 gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca
    14881 aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg
    14941 accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct
    15001 ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca
    15061 agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag
    15121 gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga
    15181 cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta
    15241 gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc
    15301 atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac
    15361 aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc
    15421 aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt
    15481 tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact
    15541 gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac
    15601 caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt
    15661 gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc
    15721 gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt
    15781 tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc
    15841 attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa
    15901 gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca
    15961 gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt
    16021 aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt
    16081 ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc
    16141 gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca
    16201 ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa
    16261 gaatagatgc cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct
    16321 tttttttcta ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga
    16381 ttgaacaagc tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt
    16441 cctccctttg tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc
    16501 gcactgtcag ttcgattgga tctcccagag gggctagttt tcggataact atttttacat
    16561 ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta
    16621 cgacgatccc agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag
    16681 cagtcttttt attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac
    16741 tttttaccag taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg
    16801 caacgatccc gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga
    16861 cttgataaac gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt
    16921 gaaataaatc atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca
    16981 aataaatgag aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact
    17041 tgtgatatta tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt
    17101 catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt
    17161 tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt
    17221 agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata gataataaga
    17281 atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc
    17341 aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata
    17401 tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg
    17461 tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg
    17521 ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag
    17581 caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat
    17641 ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa
    17701 tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca
    17761 aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc
    17821 aaatacgatg acttttctat ctattatgga aaaaatcaat tacttgatcg atcaagaata
    17881 tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt
    17941 tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca
    18001 atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc
    18061 cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga
    18121 aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg
    18181 tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg
    18241 gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt
    18301 atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt
    18361 cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg
    18421 tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc
    18481 agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat
    18541 cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc
    18601 atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta
    18661 tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga
    18721 aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac
    18781 agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat
    18841 tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac
    18901 ggatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt
    18961 tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat
    19021 cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc
    19081 catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt
    19141 agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa
    19201 tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg
    19261 cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc
    19321 attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt
    19381 aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc
    19441 atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca
    19501 agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc
    19561 gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaatggc
    19621 acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga
    19681 aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag
    19741 acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga
    19801 tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat
    19861 tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa
    19921 taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc
    19981 aattttggct agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt
    20041 ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg
    20101 tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag
    20161 ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt
    20221 ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc
    20281 cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag
    20341 aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc
    20401 ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca
    20461 tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg
    20521 agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc
    20581 tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag
    20641 aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc
    20701 cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag
    20761 gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag
    20821 aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa
    20881 aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct
    20941 tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac
    21001 atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac
    21061 tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat
    21121 caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt
    21181 gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg
    21241 aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac
    21301 atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc
    21361 gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact
    21421 gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg
    21481 aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat
    21541 aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat
    21601 gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga
    21661 taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc
    21721 caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg
    21781 aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat
    21841 tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa
    21901 aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaac
    21961 tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac
    22021 gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg
    22081 tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag
    22141 ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga
    22201 gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc
    22261 cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg
    22321 aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt
    22381 ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg
    22441 tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta
    22501 tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg
    22561 attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg
    22621 ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg
    22681 agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg
    22741 gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat
    22801 tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca
    22861 atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct
    22921 cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa
    22981 actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt
    23041 gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc
    23101 atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa
    23161 aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct
    23221 ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa
    23281 tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca
    23341 acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac
    23401 aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc
    23461 aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt
    23521 acttttcata attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt
    23581 atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt
    23641 tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa
    23701 gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc
    23761 cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa
    23821 ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc
    23881 atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata
    23941 taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc
    24001 ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat
    24061 ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct
    24121 ttttacaata attagtatag accaaattgc atttttttca ttttcttgac aataaatagg
    24181 aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa
    24241 gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta
    24301 tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag
    24361 gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg
    24421 gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc
    24481 attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat
    24541 taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa
    24601 ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg
    24661 ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg
    24721 agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa
    24781 gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc
    24841 tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt
    24901 ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc
    24961 agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc
    25021 taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg
    25081 ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc
    25141 gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc
    25201 cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag
    25261 cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt
    25321 tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta
    25381 ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag
    25441 ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac
    25501 cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac
    25561 tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg
    25621 gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc
    25681 tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac
    25741 agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg
    25801 tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg
    25861 agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg
    25921 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc
    25981 ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag
    26041 atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag
    26101 cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga
    26161 accagccgca cctccaataa agtacataaa cataaataca gtattatttc ggttgcttgc
    26221 tgcttcacct agcccctgta ctcgtgtttg attggcgact tgaccaaatt gattcccgac
    26281 gtctaataaa ataatagcta aaagcaaaat ggccaaatgg cttccgccaa ccaataaagc
    26341 aataaagctt attgtttgca taacaagtcc catatacacg atttttcttt cagaataaga
    26401 atctgccagt cgtcccacga taggtgcagc aactgctcca gacaaaccaa aaatcgctaa
    26461 tatccctgct tcgaatgttc cccaattgta ggcgggactg ctgatataaa aaatcaacgt
    26521 agaccaaaag atcgaaaatg ttccaaacat gaaaaatcca ctgactgccg cttctcttaa
    26581 tagacgttga gaagcaacta aatgcggcaa acttttaagt gaatcaaggt atgaaacagc
    26641 tgtactactt tgctggacag aacgcggcat tttccagtaa agcagaagtg taagaattcc
    26701 gactgcacat aaagcaaata aatagatcat ccgccaagaa aaaagactgg caacaatacc
    26761 agataccgtc cgagaaagca aaatccccac gagtaaaccg ctaagcaatg tacccatgac
    26821 cttccctctt gcttttggtc ccgctagtac cgcaccgtaa ggaatgatga tttgcggcac
    26881 gatcgaaagt aatcctatta aaaaagaagc gcatgcaaat aacgtaaacg aaggcgcaaa
    26941 aaaagcggcc aacagagaga cggcagataa cgctgccatt cgaatgatca attttggacg
    27001 atccaccacg tcccctaaag gaactaaaaa cagtaaccct aacgcatagc ctaattgggt
    27061 caacatcgtc aatatcccga ctgcacttgt gctaacagat aaactagctg ctacttttgt
    27121 tccaatcggc tggatgtaat acatattggc aacaacaacg ccacaagtga ttgataatag
    27181 taaaataagc gaagttgtta aaggtttttt ttcattactc ttcattgatt tcccccattt
    27241 cctatcttag tttacaactt atggaaaaga ttggaaatag aaaaatacaa ctattcgcca
    27301 cacttattat ttgtgctttt gttttccttg ttttttcttg cgatctttat aaatgaagac
    27361 aactaaccct actagcacag taacacttgc aaccaacgtg actgcagttt tgttatatcc
    27421 gtgaaacaga gcttctccac cacacgcata caacacagac gaagggatga ctccaatcac
    27481 gatcgacaat atgatttgct gtagaggcaa tttcatcgca tctgcagcaa aattgaccat
    27541 agaagatgga ataaccggta tcatatagcc taacattaca cctatttttg gatgtttcat
    27601 ttgtcggatt gcttgcaccc aatgattcgt ttcggttttc ttatctaaaa attttaagcg
    27661 atgcataagg aaaattgcag tgatgttacc aagagcatta cccaaaatgt tgatgatcgc
    27721 acctgtcagt ggtccaaagc tcaaaccagc aatgactcca actactgaag taggaatacc
    27781 tggaacagca cacatcaccg ctgtcaatac gatcaagagt atcgatgcaa aagtcccatg
    27841 cgaacgaata tgtgcgagta gctctgcctt actcgctctc ggatttaaaa ataattggat
    27901 gtctgtctga tattctaaat acaaccgata gcccactacc agtatcagaa tgattcccaa
    27961 cgagatcaat atcaatcgga ttattttctt tttcatattt cctcctgaaa aaaatctata
    28021 ttcattaaat aatagtttaa cataaaaaca aaaaagcacc gaaatccaac ataggatctc
    28081 agtgctttta aatagtaaaa attatttaac agttacagat gcgccaactt cttcaagttt
    28141 agctttcaat tcttcagctt cttctttaga tacgccttct ttaactggtg caggagcgcc
    28201 atcaactaca gctttagctt ctttcaagcc taaaccagtt gcttcacgaa ctgctttgat
    28261 aactttaact ttttggtctc cagctgcagt taattctaca gtgaattctg tttgttcttc
    28321 agcagcagca gcgccaccag cagcagctac agctacagga gcagcagcag atacgccaaa
    28381 ttcttcttca atagctttaa ctaggtcgtt aagttctagg atagttgctt cttttaactc
    28441 agcaacgatg ttttcaatgt tcaatgccat tataatttcc tccgtttaat tgttcttttt
    28501 tatttaatgc tttatgcggc aattaagctg catctccatc ttctttgtct gccactgctt
    28561 tgacagcgta agccacgttg cggactggcg cttgtagtac agatagcaac atagatagaa
    28621 gtccttcgcg atttggcaat tttgccaatg ctgtgatttc ttccaatgaa gagactttac
    28681 cttcgatgat accgcctttg atttctaatg cttttgcttc tttagcaaat tcgtccatga
    28741 ttttcgctgg tgcgactaca tcttcgttac tgaatgcaac agctgtaggt ccagtgaata
    28801 cttcgtccat gccttcaagg ccagcttttt tagctgcacg tgaaagaata gaatttttga
    28861 taactttcat ttctacgtta gcatcacgaa gttgtttacg taaacgtgtt acttcatcaa
    28921 ctgttaatcc gcggtagtca acgactacta ctgatgcagc tgctgtaaat ttttcgctta
    28981 cttcatcaac gattgctgct ttttttgcga taattgcttc actcattttt gagtttcacc
    29041 tcctgaattt tgatggaaga gttttttgca ctgaaaaggc aagtcacttt tatagttact
    29101 ttgctcaacc agtattattc atcacctatt cacttcgttc atagtgattc tactcgcttt
    29161 gctcgaaagg aaactccttt cgctttgctc aaccagtatt gttcatcatc tcttcacttc
    29221 gttcatagtg attcacaata aaaaactcta tgccaccgca gacatagagg gactattgaa
    29281 tatattcaat aaacgtcctc ggtaggaaat taaggcttga tgccacctac tgtcttcggt
    29341 acaagttaat tattaacctc aactagctta gcatgctaaa ctagttgagt caagaattat
    29401 ttttcagttg gatcaatcaa cattctaata tttaattaga aagaagcttg gtcaacatgg
    29461 ataccaggtc caaatgttgt tgtgatcgtt acatttttca tgtattgacc tttagctgct
    29521 gatggtttag cttttagcaa tacatcatgg attgctgcaa agttttcaac taatttttca
    29581 tcgtcaaatg aaacttttcc gattggtaca tggatatttc cagctttgtc cacacggtaa
    29641 gttactttac cagctttcac ttcgtttact gcttttgtta cgtccattgt aactgtacca
    29701 gttttagggt ttggcattaa gccttttggt cctaatactc gtcctagacg acctacagta
    29761 gccatcatat caggtgttgc aacaactaca tcaaagtcaa accatccgtt ttggattttt
    29821 tgaaccatgt cgtcgtcacc tacgaaatct gcaccagctg cttcagcttc ttttgctttg
    29881 tctcctttag caaaaactaa aacagtttgt gttttaccag ttccgtttgg taatacaaca
    29941 gcaccacgga tttgttggtc tgctttttta gggtctacgt tcaaacggta tgcaacttca
    30001 actgttgcat caaattttgc aatgtttgtt tcttttgcta aagctactgc ttcggcaaca
    30061 ggataaactt tacttgaatc aacttttttt aatgcatctt gcattttttt gctcttttta
    30121 gccattttgg ttcctccttg attgtggtta taacggttgt acctcccacg tgttctatat
    30181 ctttcaatat agagccaact gagaagctgc tgtcttaggg tgtatagatt attctacagt
    30241 gatccccatg cttcgtgcag ttccttcaac catgcgcatt gctgcttcaa cgttagctgc
    30301 gtttaggtct tccattttta attcagcgat ttctcttact tgatcgctag aaactttcgc
    30361 aactttattt ttgtttggtt cgcctgaacc tttttcgatt ttagctgctt ttttcaatag
    30421 aacagcagct ggcggtgttt ttgtaacgaa tgtgaatgaa cggtcttcgt atacagaaat
    30481 aacaaccgga atgatcaaac ctgcttgatc agctgtacga gcgttgaatt cttttgtgaa
    30541 tcccatgata ttgatacccg cttgacctag tgcaggacct actggtggag ctggagttgc
    30601 tttacctgca ggaatttgca atttaacgat tttttctact ttctttgcca cgagacatac
    30661 ctccttaagt ccgtgatgtg gttaattgga gatgcatatt tctcctccca cgttttatct
    30721 gatctgaaaa aatccagaca aataatgtgc aaaacaatgc acactggtac agtatagcaa
    30781 atttatttgt tttttcaaga tttttttctg ctatttttac attttgcttg aataaacgct
    30841 aaatttattc taaaaaaaca acaatttttc gttctcattt cttcttttat gttacacttg
    30901 cgctagcatg gttcaacaga tgaaagcaaa caataacacg gttttttaga cccttgtaca
    30961 aagattccta cataacaaaa tgaggtggag aaaatgaaag atttaaggta tagaagtgtc
    31021 tttgatatta ttggacctgt gatgattggt ccaagtagct ctcacactgc cggagctgct
    31081 agaatcggga aaatcatacg tagtatattt ggcgaacagc ctgattctgt agatatttat
    31141 ctatatgaat catttgcaaa aacctatcgg gggcacggaa cagatattgc attagttggc
    31201 ggcctgttag ggatggaacc cgatgatgaa cgtttagctg attctttgaa attggcatat
    31261 gaagccggta tggaagtctt gtttgttcct aagagtgaaa aagccgaaca tccaaactct
    31321 gtaaagatgc ttctaaaaaa aggaacacga aagctttcag tcactggcat ttcgatcggc
    31381 ggtggcaata tccagatttc tgaattgaat ggattcaaaa tttctttaag catgggtact
    31441 cctaccttga tcgttgtcca ccaagatgtt ccaggtatga ttgctaaagt gactagtata
    31501 ctttctgaga ccaatacgaa tatcggcact atgacagtga cacgggaatc aaaaggtgaa
    31561 aatgccatta tgataattga agtagatgat cctcaagtag aagaaacggt aaaaagactc
    31621 aaacagctgc caaatatcga cagcgtaaat tatttcgatt aaggagctaa aaaaatgttt
    31681 ttatcgattg aagaattagt agatcaagca aaagcttatc caagtgtagc agaattgatg
    31741 attgcagttg aaatggaaat gagtggccgt tcccgtagtc acatcatcga gatcatggaa
    31801 aaaaacctct cagtaatgga acaatcaatc aacgagggaa aagaaggtgt tacctccgtc
    31861 acaggaatta ccggtggtga tgcaaaaaaa ttagatgatt atttaaatga ggcagacttt
    31921 ttaagtggag aaacaatctt aaaagctgta cgcaatgctg tagctgttaa tgaagtcaat
    31981 gcaaaaatgg ggttgatttg tgctacaccg actgccggaa gcgcgggtgt tgttcctggt
    32041 gtgctgatgg ctgtccgaga tcggcttgat ttgacacatg aacagcaatt ggaattctta
    32101 tttaccgctg gcgctttcgg gttagtcatc gcaaacaacg cttctatcag tggtgcagaa
    32161 gggggatgtc aagcagaagt tggttctgca agtgcaatgg cagcagcagc tttggtttgt
    32221 gcaaaaaatg ggactcctca tcaagcagca caagcagtag ccattacatt gaaaaatatg
    32281 atgggactga tttgcgatcc cgttgcaggc ttagttgaag tcccgtgtgt caaacgtaat
    32341 gctttaggct cttctcaagc ttttatctct gctgatatgg cattagcggg tatcgaaagt
    32401 gtcattccgc cggatgaagt cgttgctgca atgtaccaag tcggacgtca aatgccgtcg
    32461 attttcaaag aaacagcaga aggtggctta gctgttacgc caactgctaa acgacttgcg
    32521 gaagagattt taaataagag ggcgtgataa agctccttaa tttcgagtat taagagatta
    32581 gttatcttca taacaaaaaa cgaagagagg actaattgcc cttctcttcg ttttttatta
    32641 atccatcaat ttcgtatatt ttacttcatc gatcgttttt gttcctgcaa gttgcatcgt
    32701 gatcgacaat tctttgttca ggtgttcaaa aacagatgtt actccttctg ccccacctag
    32761 attcaaccca taaatcactg gtcggccaat cgcaactaag tcagcaccac ttgccagagc
    32821 tttgaagaca tgctctcctc gacggacacc agagtcgaaa atcactggta cacgtttgtt
    32881 gactacttcg gcaatttttg gcaatacttc aaaggacgca ggacctccat ctagttgacg
    32941 tccaccatga ttggataccc agataccatc tgcccctgca gagatagcga ttactgcatc
    33001 ttctggcgat tgtattcctt ttacgataac tggaagatgg gtgatttctt tgattgtttt
    33061 gatgtcatct ggtgtcaagc cttgttttgc agctgcataa atttctgcga ttccttttcc
    33121 ttcgccatta ccacttgcac tttgttcgct atacgcagct aagttaggca ttggtaatgg
    33181 aaattggaat tgattgatga cgtcttcttc acggtaacca ccgagcgttg aatctgccgt
    33241 taaaatgata gctttagccc ctgcttctac tgctttgttc aaaatgaact cattgaatct
    33301 gtcgtcttta ctcatatata attggaaaaa ctgcggtgcg cctggcgctg catcggctgc
    33361 gtctttgatt gtcgtatttg cataagtgct gatggaaaag attgatccag cagcagctac
    33421 tcctttagct gtatcagctt cccctttttc atgtgccaat ccttgggccg ctgatggcgc
    33481 ttggataatc ggtgtatcta attcaattcc gaaaacagat gtatgcaaat cggctgagtc
    33541 gattccgcgt aatatccgtg gcatgatttt ctttgtatta aaagaagtcg tattttcttt
    33601 catcgtccac tcatcttcag aaccaccgcg aatatagcca aaagctcctg cttccatgcg
    33661 ctcttttaca cgaccttcta gttaggcaat gtttacaata tcgattggat gttcttcatt
    33721 acttgtatga taagtcatag tagatccttc tttcatcttc ttgattacat ataaatcata
    33781 ctaaacttac aaaaagtaag caaacattta ctctcagata cttttatcgc tattctttcc
    33841 aaataaatcg acttacaagc tgatctactt cttggttttc atgaagcata ctatgagagg
    33901 cattttcacc tgtcactact ttttcctcta tttcatttcc gcgttgcttc aacaacgcat
    33961 agaccgctaa tgcactgttt aacggtactg ttccatcact caagtctgtt tcatctagtt
    34021 gaccagcaat caagaaaaat cgagtggtta taggaatatt accgatcatt tgctggtaat
    34081 cttgatagcg gttagaaacg acagcaggcc cctttgctaa ctcatctgtc agagactgcg
    34141 cactatcatc gacaaaatca ttaaaaggcg cgccaattgc tgttaatgtc tgaacttgtg
    34201 gcaatgacgt atcctgtcca tacgttccta aatagcgtag tgcactaact cctcccatgg
    34261 aatgccccac tatgttgact ttatctatgc cgtagttttc tttcagatac aaaagacaag
    34321 ttttgatcca ttctgcttga ttccactcgt tatttttatt atcttcaaac aatacttgga
    34381 ccatcggatt gtctgctttt ccagacaaat ctcctgttgc ttgtatttgt ccatctgttc
    34441 ctactgttaa aaccaattct tttactgctt gctgattttt ttccaaacgc cgcaacatcc
    34501 ctttaaagga attttctgtc ccgctgtagc cgtggaaaaa aagtgttgga atagtggttt
    34561 tttctatcac tttactggat tctgccgttt tttctttatc agaaacttca gtagtattta
    34621 tcttcgtcgt ctggcaacca cttaacgcaa acaaactgat aaggactaaa gctgctgcag
    34681 ttgcacgttt gcataaattt ttgattggtt ctgtcataca cctcttcctt tccatataga
    34741 aaaaagaaag acttgaaagg ccaatcatat gatcgacatt tcaagtctct ttattaatcg
    34801 attttatcta cttgatcgaa atcaagttct gtactggttt cacgtccaaa catatcgatg
    34861 ttgactttca atttttcttt ttctccgtca acttctgtta cttgtccttc taatccagca
    34921 aatgcgcctt cgatgatttt cacgacttcg ccaacttcta cttctagatc attttgtcgc
    34981 aggctcatac cgatagaacg caagatatga ttgacttctt ctggaagtaa tggtgctggt
    35041 ttgcttccgg ctccgtgtga accaacgaat cctgtcacac caggtgtgtt gcggacaaca
    35101 taccatgaag cgtctgtcat gatcatttca acaagtacgt atcctgggaa tgttttatga
    35161 acgatttctt tttctttccc atttttgact tcagtttctt tttcttcagg aacaacgaca
    35221 cggaaaataa agtcggccat tttcatactt tgcgcacgag attcgatatt tgcttttact
    35281 ttgttttcat atcctgaata tgtatgcaag acataccagt ttttttcaaa agattccatt
    35341 gaaggctcct catttcattg taaaataaaa aaacctcagt acacccgaag tttttctttc
    35401 gcttcattat agcactaaaa gtacgttgtg gctagcgagt tattttaaaa tccagccaaa
    35461 taagctttgg atggctgtat ccattacgta aaacatcaat ccgaaaataa tagaagtttc
    35521 gatgactact aacgtatctt tacggagttg ttttctgctt ggccaagtta cttgtttgat
    35581 ttcatctttt acgcttttta gaaatttcat gagacaacct cccttgctac tgtttatttc
    35641 gtttctttat gtaatgtata ttgattacaa tatttacaaa acttattgat ttccaaacgt
    35701 tcgccgcttt ttccttcact gactgatttt gtgtagttgc gagacccaca aacggaacaa
    35761 gcgagtgctg cttttttctt tgccatttac ttctgccccc ttgctgagaa tcagacttat
    35821 acttcataac agtatcacgt aactttttac ttgtcaattt gtgaaataaa ctatctattg
    35881 ttttggataa aattatgata tgattaattt gaaattaagc taaaatgaga caattggagg
    35941 tatagcgatg ctattaaaaa cacttgtata taagaaaaaa gatctgacaa ccgttacaga
    36001 gactagtaca ttagaagaag cattaaaaat tttagaaact tccggatacc gttgtgtgcc
    36061 gatcttagat tcttcaggta atattttccg tggaaacatt tacaaaatgc atatctatcg
    36121 tcataaagca aatggcggtg atatgacgct tcctgtcact catcttttga aaaacgctac
    36181 aaagttcatt tacctggata cttctttctt caaagtattc tttacgatca aagagcttcc
    36241 ttatattgct gtccttgaca gtgaaaatca tttttatggt attttgacac atagtacgtt
    36301 gcttaatatg ctctctcaat cttggagcgt agaacaagga agctatgtac tgacaattgc
    36361 ttccacagga aagcaaggag atttggctgc tattgcaaaa atcatttcaa aacacagcag
    36421 cattgccagc tgtattactc ttgatatcga aaaagacgaa tacattcgac gtacattgat
    36481 cacgctaccg gctaacactg aagcgaaagt atgtgaaacg atcgtcaacc acttagagaa
    36541 aaagaattat aaagtggtag aaattgaaaa tttgcaaaac caagaagcat aatcaaaaag
    36601 cgaaaaaaga aaacagctaa tctgctttct tttttcgctt tttataatta gacacgcttt
    36661 tcaaaatcaa gcgcgcgatc taaataatac atcaatggtg ctgaaacagc catgacgata
    36721 aactcactta atgcaagcgt gccataggtt gtccaaaatg cttggccacc tgttggaact
    36781 aacatataag caatcagtcc catgctgatg gtaaagaaga tcgtgttcaa tactaaacgc
    36841 atctttacat ttttcacttt gttttgcaat aaagctgtca ggcctaaaga aagcaatgtt
    36901 tggccgccac caaaaaccac atccatgata ccaaaaccaa agaaggcatt atagacgatg
    36961 accccgccta aaactcccca aagcaattta cggttaaaga caactaaatg attcaaactt
    37021 tctgaaatac gaaactggat cggccctgag gatacaggcg caactaaaat agttaacgct
    37081 agataaagag ccatgatgat gccatttaca acgattacgg ataaccgttg tctgcttttc
    37141 gttttttgca ttttctctcc tcttttctcc tagttttttt aacgcgggat ggttgatgaa
    37201 ccgcggttat gtgaaacaaa caataattat aacagacttt tcatttattt caatccgtct
    37261 tatctgcttt taaaaaagca aaagcttaaa caatgtaaac gtttgctaaa aatcgtggga
    37321 tatggtataa agtaagtgta aggggtcata agaattccca gtaaagcaga cgaagtagga
    37381 tctgcaagaa cagaattgtt gtgaaactta aataaataaa aaaggatgtg ggaatttgag
    37441 caacctctta ctagagaacg gtctagcaag cagctgaaga tctaagtgga catgccgaac
    37501 cttggaactt ctccttacgt aagtaatgga tcatgcgctt gctaggcttt tttgtgtttt
    37561 tcggtcttcg gacctttttc attttcctag acttctgcta taataaccaa gaattaattt
    37621 tgtaacagat ttttttgaaa aaacaagttt ctttcattca aaaaatagaa aaagcttgct
    37681 aattattggt taaccagcaa acaggagtat taaaatatct tgttttattg aatagcagga
    37741 ggaaatcatg aaattccatt atcatatatt gatcaatcca tcggcaggca gcggaaatgg
    37801 ctataaagta gcagaaagaa ttttaccggt attaaaaaat aagcatattg actatacact
    37861 tcattatagt gaatacaaag gacacgatgc tgagattgct gaaactttgg caaaagaaac
    37921 attgattcct tggatagaag aagaacagaa aacaatcgat actttccccc ttttgatcat
    37981 cgtaggaggt gacggcacct tacatcaggt acttgatact ttctatcaga tggaagtaga
    38041 attcccagtt gcttatattc cagcaggttc tggagatgat tttgctcgag gggcaggcct
    38101 tccaaaaaat cccaaaaaag gacttcagct gattttagca gctcaatcac cagaaaaagt
    38161 ccacataatg gcttatgaag aaaaaataag tgaaaaaaaa ggaatagctg ttaataattt
    38221 cggtatagga ttagatgcag ctatcgtcca tgcgacaaat cattcttcca caaaaaagcg
    38281 attgaacaaa tacaacttag gatcattttc gtatttattc tctattctcc gtgctttatt
    38341 cactcaaaaa ggatttccaa ttctggtaga tgcaggagga aaacaattaa gtttttcaaa
    38401 cgcattttta tgtacagcaa cgaagcatcc tttttttggc ggaggaatcg cgattgttcc
    38461 aactgctgat gcgtctaaac cagtcattga ttttgtcgtg gtcgaacgaa tcaatttctt
    38521 taaaatcttg tggctcattc ttttacttat acagaaaaaa caattgaaat cgaaatattt
    38581 ccatcactac acaacaaaca gattacgtat cgtttcaaca acaccgcaac atggtcaaga
    38641 agatggagaa gaaatggaaa aacgaccttt tgatatcaca ataaccacaa ctaaacagtt
    38701 gttttggtgt taataaaaga ggttttgtca gaagctacat ctggaacacc gtttattcgg
    38761 aaaaaaggac tgtgacacga cttttgtcac agtccttttt ttactatgca gttaattctt
    38821 tgcccaattg ggttgccatc gatagtttct ttctttaccc aaaataaatt ggatcattga
    38881 aaacaccgca gcaaaagcaa ctaagacaca accattgaac caaaaagatt ctgcaaatgt
    38941 ttgattgtaa ctgagtgttt cactgatcaa gttattattt tgaacaaacc aatatgttgg
    39001 tgtgaatgcc gctattttgc taaccatttc tggcaagtat tctgctggta caaacacccc
    39061 accgataaag caactgcctg tgataaaaat atctttgatc ccaccgctgg tttctgagtt
    39121 ttttaccaat gaagtgatac aagccgaaaa gctaatgatc ggcaataaaa acaagaaagt
    39181 atttaaaata tgataccata ccgttatatc ccacgaggtg tggatcacta acagaaccat
    39241 tagaagtaag aagccactga acaacagcag cgagtaacta atcaaactaa aggttacttt
    39301 gcgattcaag tttcgtttgg taatgggcgc actctggttt cgtttactga tctctttgcg
    39361 attaaatgct agattgatga cgctgaaacc gctaaaaatg gatagaaaca acccataaga
    39421 caagaaatta aaggttgtgc ccgtcaacag ctttttcatc cgctgactat atcccgcact
    39481 tagctcaact tctccagtaa ccgagagtgc catttgtgtc tgttccatag ctgcatcgat
    39541 tgttttatct tcttcttgaa acagaatcag tgtttgcaaa aaggtgttca cttgctgtgt
    39601 aacaactgtt ttagaaaagg catctgggcg actctgtatc ttgatcgtcg gcatctttcc
    39661 tacttcgact tgttcggtaa agtctgcagg cagatgtaag ataatgttta cacgattaaa
    39721 ataaagagca tcatcgattt ctttttgtga ggttccatcc aatgtaacga ctgtttcctt
    39781 gtcattcaaa tatttacgaa ggccatcgac tacaggcgac tcttcttcac taatcaaggc
    39841 gattttcgca tcttgcagag aggtattttc aacatcaata ttttgcgcaa aaaagaatgt
    39901 ggcaacaaca gtgataatta cacctatcag taaattcgat ttatttgctt taagcaatgt
    39961 ccaaatcgtt ttataaagcg tcataacgca cccctctttc catccaagca tttcctaacg
    40021 caaaaaggaa aataaatccg atcaaccata gaagattggt ataaaaacta gccaaatcag
    40081 tgtagaaata aagtttatac aaactttcgc tgatcaagtt gacgagattt aattgaccaa
    40141 caatcggaat atgaagatca atccaatact tcacttgttc cgaccccatc ataccagcaa
    40201 aaaagctcat cgcaatcgtc actgaaatca agatgctatc cttttgcacc agattcattt
    40261 ttggtatcaa attaccaacc aatgtaccta acaaaatcgc acacaacgac cccagagcgg
    40321 taacaatcat caaccattgc caacgttcac caaagtctac ttgatagata aaacgaaaga
    40381 cagctaacat aatataaatc tctgtcaaaa caatcgtaaa gcttgctgcc aaattagcga
    40441 ttgaaactaa cgattgcttt ctaggaatca aggacaagcg aataccattt gacgattgat
    40501 ttgcttgctg atcctgtaca ttgcggaccc cccacatcgt cccgtagaga atcgccattc
    40561 caatcagagt gaagaaataa aaacttttga aactcatatt acgacctgca tgaacttctt
    40621 gaaaagatac ttcttgttca aaaacagtca tgatgggcgc gagatcaacg ttctccactt
    40681 ctgccacttt actcgaaatt ataccaatac gctgtaaaaa ttgatccatg acgtttttca
    40741 tcaacgtctg actcattcct gcttgaccca catggagcga aacagatgaa ccatcaatga
    40801 gataaaaacc agccaaatca tctgcattta gcttttcatt cgcttctact tccgatagtt
    40861 cctgtacttc aaataatggt ttatcattga cttcgacttg ttctaagact gtggtaaaat
    40921 tttccgaaag cgctgtttga tccgctttga ttacaattcc agtattaatc gtttcgagat
    40981 tgccaccttg atcaatctcg ccaaaggccg ccataaacat caatcctaaa agaattggaa
    41041 aggccaatgt ccaaaacaac atggatttat ttcgcagcag tatttttgtg cgatatttat
    41101 aaaaatgcca aaacattatt tcccctcctc tagtctctca aatccttgcc agtaatctct
    41161 aaaaagacat cattcaagga cgcttctttg gttgctattt ttccgtaact gatcttttct
    41221 tcttgtaaga ttgtaagaat cggcaacaat actgtattta gttcagtagt tgttacttgg
    41281 agctgacctt ttttataagc gattccatca gaagctattg ttcgctgaca acacgttttc
    41341 tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat cttttagaga
    41401 ttctttggtt cctttagcaa tcacctgtcc ttgatccatg atcacgatct ggtcgcaaag
    41461 aatttctact tcttccatgt aatgtgttgt atagacaatg gttgctcctt gtcgattcag
    41521 ctccttgata ctttctaaaa ttttattccg actttggggg tctacagcta ctgtcggctc
    41581 atctaaaaaa attaaccgcg gcttgtgggc aatcccacat gcgatgttca accgacgttt
    41641 caagccacca ctaagttctc ctggacgaaa cgaaacatat ttttctaatc ccactaattg
    41701 aatcacctct tcaatcaagg ctttcctttt ctctttttcg gaaacatata aaccgcaaaa
    41761 ataatcgata ttttcttgca cacttagttc gtcaaagacc gcaacgtctt gaggaacgac
    41821 cccaatcatt gccttgacag cataattttc aggagtcatt gttgtcccga acaatttaat
    41881 ggttcctttg tcataattta acaaagaaag catacagttg atggcagtcg atttcccact
    41941 gccgtttggt cctaacagac ctaagacttc tccttcttgt acttgcagat caaaatgatt
    42001 caatgctaca aaatctccat agcgtttgac taatttatca atttcgataa tcatcatgaa
    42061 aaactccttc caaatttctt gttgatctga ctataataaa gtgcaagaaa agaaactagt
    42121 gacgattggc taaaccacac atgacatttg tcacaatgaa ggagtctgct atgcataaat
    42181 ctcggctact caacgaaact agctttctac tggttatctc attgcttttg ctggtaagtc
    42241 aaggattcgt tgctatgcaa gttatctatt tattgatcgc cgttttattc actggtgtca
    42301 gttatttact gcctacaaag ccaaattgga ttttccaagt tattctattt cttttatgtg
    42361 cctttttacc tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc gcccctgaag
    42421 aaagccacct tgattacttg tttttgttag gttttttgtt gatttcgacg gttggtaatc
    42481 atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt tacttacgaa
    42541 aaaaagatca acgaacgacc gatcttgaga aaaagtacat tgtctccaaa gatactcatt
    42601 gggaaaaaca acaagagtta tcggaacaaa ataaagcatt ggccctgtca caagaaacac
    42661 aattagcctt gcaaattgtg gaagaacgga accgaattgc ccgagacatc catgacaatg
    42721 tcggtcatct gctgtcaagt gcgattttac aaattggtgc gcttgaaatg atcaatcaag
    42781 aaccaaaact aaaagaacca ttagaaaatt taagtgccac cgttcacact gggatggatc
    42841 ggattcgtca aagtgtccat gacctccatg agacatcgat ctcttttcaa cactccttag
    42901 agtttctgat cgctgatttt ccttcttctg ttacgattga aggaaacttg tttgaaggat
    42961 tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta accaacagta
    43021 tcaaacatag tcatgccaaa accgttgttt tacattttcg aacgttgccg gccttttatc
    43081 gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc ggtattggtt
    43141 tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca agtccatatt ttccaagaaa
    43201 accagcaatt tcagttaacg atcattttac caagggaggg acaccaatga taacagtagt
    43261 cattattgag atgatccatt tgtgacgaaa tcgttacaaa ccattttaga aagcacgaaa
    43321 gagatccgtg tgttaggcat tggtcattgt gccaaggatg cgctagtctt atacgaaacc
    43381 catcgtcccg atgttttgct gacggacatc cgcatgccta aacaaacggg catcgatgct
    43441 gccaaagaaa ttttggctaa atttcccaaa gccatccttt tgcttttgac gacattcaaa
    43501 gatgaagaat acatccgcga agcattttcc atcggtgtca aagggtattt aatcaaacag
    43561 aacttgcaag cgattattcc ttctgtcaaa tccgcttata atggacaggc cgtatttggt
    43621 aatgaaatca tcgagacttt cactcaaatg atgaagaata aaccgacgat tgatagtcat
    43681 gcttatgctt cttttagtga acgtgagctt gcaatcatca aagaagtagc tgccgggaaa
    43741 aacaataaag aaattgcaga tgccttgtac ttgagtgacg gcactgttcg caattacatc
    43801 agccaattac tagaaaagct agatttacgg gatcgcacgc aattggctat ctatttttat
    43861 cagcatttgc aataagtctt atctaacaca aaaaaacctg aatgatgctc cttcgcatca
    43921 ttcaggtttt tttcacatct tctattcaat tgtcccgcaa accatttcct cacgattgaa
    43981 agaatcaagg attcgcaggg ctgccaggat tcgttggatt gttcggcgtg ttaggcggaa
    44041 aaataggcgc cttgtctaac gttttttcta gttttgattc ttcacgatcg tctcgttcta
    44101 catcaggact ttcattcaat ttgaattctt tttccatctc atctaacttt ttaaagtcat
    44161 tgccagattc ttttgctaat ttcgctaatt tatcttcggt ttctttagcc ataaagtaac
    44221 gccccccttt tgatttcagc ttagcatatc acactgaaaa aacaaaagaa aacgctaacc
    44281 aacgatcatt tttagctatt tctcatcttt aaaatcaagc attttttgcc ggtataaaat
    44341 cgtagggaga actgttttcc ataccaatca tcggatctac taactggtta tcgatcactt
    44401 ctgctgtaag aacgaaatct ttagaaacag caggttctac ctcttctttc ttttcttctc
    44461 caacaaatga agaaaacaga tctggttcca ctgtttccat tacttggttt ttcgtttcat
    44521 tcgtcccact gtcatcttct acaggtagct tttcttttac aacccgtggc tcttttgtat
    44581 agttcccatc ttcaaatgga tcatttccga ccaaagaatc ttcataagct gctagtttgg
    44641 tccggagagt gatattcatt ttatcattat ccatgatcct tgatttcaac gtagtcatcc
    44701 ggcttgctga ttcattcgct acacaagtga cataggcttg ctcttcccct aacaaaatca
    44761 acagttcctt actgcgagtc actgccgtgt agagcaaatt acgctgaagc atccgctgat
    44821 attgatggac catgggcaaa ataaccatct tgaattcact gccttgtgat ttatggattg
    44881 agcaacaata agaaagagtg attttattcc attcgtttct tttataagtg acctcatttg
    44941 catcaaactg gataactaat tcatccactt tatcttcaga atcttttgcc aagatgatac
    45001 ccgtgatttc tcccatatct ccattgaata cattcaattc gggagtattg actaattgga
    45061 ggactttgtc tcctatccga taaacggtgt cgttccaatg tacttccttt tttccaggtt
    45121 tccctggatt gaatatctcc tgcatcattt tatttaacgc atcgatccct gcaggtcccc
    45181 gatacattgg tgctaatact tggatatctt gtgcacggaa tcccttattt ttcgcttttt
    45241 ccacaatttt agagatcaag ggttcaattt gataagcatc acttcggaaa taggaacgat
    45301 ctttttgatt ctttgtgaaa tcttttggca gacgtccttc tttgatctca tgggcaagag
    45361 gaataatact agaaccatca ccttgccgat aaatctgatt gagttccatt ttgggaattt
    45421 gttcgatttc caaaagatca tggagtactt gaccaggacc gacagaggga agctgatctt
    45481 tgtctcctac taagataact tgcatattcg tcggaatcgc tttgaataat gtgttagcca
    45541 gccatgtatc gaccatagac atctcatcaa caatcaaaag tccgccttct aattctttcg
    45601 tactgactga aggtgttttt tcccgtccat tcagccccag aagacgatgg atcgtactgg
    45661 caggcagacc tgtcgtttcg ttcattcgtt tagctgctcg gccagttggt gctgcaagta
    45721 gaataggaaa aatttcttgg gtatattttg aaggatccaa ttccagtccg ttcaactctg
    45781 caaaaagatt gacgattccg ttgatcacag tagttttccc cgtacctggt cctcctgtta
    45841 aaataaatag aggggaacgg attgcttcag cgatagcttg ttcttgcgaa ggtccatact
    45901 gtatgccgaa tattttttcg atacgccgaa tattcttctg cacttctttt tcaggataat
    45961 ggatttcttt tttacgactg agaaggcgct ggatcgaatt accgatcccc cattcagaaa
    46021 agtacaaact gttttcgtaa agattagtct cttcttgttg tatctttcct tcttcaacaa
    46081 gttggataat tacagtagca acctgatcag gctggatttc aattgggcga cttgtttcta
    46141 aaatacggat cgtttgctga agcaatgtct ccgccggtac gtatgtatct cctgtttgaa
    46201 ctgcatgctg aaaaatctga tgcaagatag ctgctcgaat tcttctcggt gaatctgctg
    46261 caataccgat ttgttcggct agattatctg cacgtttgaa cccgattcct tctatgtctt
    46321 cgactagctg gtacggattt tcttcgatga tatccaaaga ctcgttctta taggcctggt
    46381 agatactgaa agctaactga ctaccgaacc cgtaacgatt cagtcccatg atcacttgat
    46441 ccatcccgtg gtttagccga atcgtctcag caagcatttc tctttttggc ttcgtcaaac
    46501 cggagatttg ttttaatacc gaaggatcag caatgatttt ttcgatagca tcttctccca
    46561 gcaattctac aattttttct gctgttttct tcccgattcc tgggaatttt tcgctggaaa
    46621 gatacgctac gatcccattt gcagttgttg gcttttcttg ctcatatctt tcgagtttaa
    46681 gctgccttcc gtatttagga tgatccacta attctccata aaaacgataa gtctcatttt
    46741 cttgaatatc gccgaaactt ccggtgacta caatctcttt ttcacggtaa tcagctgtgg
    46801 tttcactgat gtgtaccaaa agcactttgt aaaaattact aggattttga aaaaaaatcg
    46861 cggcaactgt accaacgaaa taaggcattt cttctggctg catcggaagc cgcctccttc
    46921 ttctatagta aaggttctgg tgctgtttca gttcctaaaa aatctgtttg gttccatact
    46981 ttcagttcat atggcaataa tctgcaaggc tcttcagtct ctaaaatcgt caaactacta
    47041 ttgaacaatc cacccatttc acgcaactca cttagctctt ttcctgtcat ccattgaatg
    47101 gctgcagtta aagaagctcc atgtcctacg aataaaagtg ggccatcttt atgttgggca
    47161 accgcatctg ctacaacagt ttcgatacgt gtgattgccg attcaatcgt ttctccgtga
    47221 aaaggagaag gatcatacag atctaaatga ttacgcaaat tcggcagatt atccggatat
    47281 ttctggtaca tttcatcgat cgactgacct tctaaagtac ccagccctag ttctcttagc
    47341 ttatctgtat aaaaaatctc taccggacga gcaagctctt tgttgatacc ttctgctgta
    47401 tcacgtgctc gctttgctgt actggaataa attgctttaa aaggaacata ttttaccttt
    47461 ttcccaaatg ctttgatttc ttcgtaactt tgtggcaaaa gcggagaatc gccattgctc
    47521 ccttgaaaac gtctttcctg attccattgt gtcttcccat gtcgggtaaa atatagttcc
    47581 atacgcagca aactcctgtc tttcttttat tttactctat aataaactgt ctaatccagc
    47641 ttgtcatttt catagtgatg cgtcaattct aaattaggga atccttgctg gcgaagtgct
    47701 tcatacacta tcattgcagc tgtattcgat aaattcaatg agcggacatg ctcatcattc
    47761 atagggatgc gtaaacactt ttcttcgttt tcacgcataa atgtttctgg caaaccggtt
    47821 gtttcttttc caaataaaaa gaagtgctct tctccatcag caaaattttc gtcactatat
    47881 actttatggg caaattttga gatcaaatgt agtttacgat tacctaaaaa tgttaaaaaa
    47941 gcttctaaat tactgtggta agtaatattt acatcattcc aataatccaa ccctgcccgt
    48001 tttagatgtt tgtcatcagt tgaaaaacct agtggctcga ttaaatgaag cggagtattc
    48061 gtggctgcac acgtacgggc aatatttcca gtgtttgcag ggattaatgg ttcaaataaa
    48121 acgatatgat tcatcaggat cattcctctc atatttatgt tcgtttctat tttctgacaa
    48181 tttgattttt gcataaaaaa aaacgtatca actcggtgtc agtcactgcg agtcgttacg
    48241 ttagtgaagc tcttctcact aacttttatc agaacaggaa cctgataaaa accaatgaaa
    48301 aaacaacttg ttgctaatat aacaccctca actttttttt gcaacctcta ctcattaaaa
    48361 actgctagaa aaacgttttc tcttagttcc ttttaataat tctatgtcaa ttgtaatctt
    48421 cttcaaagga ttcttatata agtctgcttt gattcgctct tctacctgcc attctaaagg
    48481 tgacatggcc aaaagatctt tttgattttc ctctggtatc tcaaattcat aggttacacg
    48541 ctgacgttca gttaatttca ttgcttcagc aaacttggct actacacgtt cattggaata
    48601 actctgtttt tcaggttgat tcggataaaa agcctgacgt agttccttta aataattttc
    48661 ttgtggcacc accttcaaca cttctccatc ttctgagagt acacgttgga attcttggta
    48721 atgagaaggc gaaaagatat ttaaaaccgc gttgaatgtt tgatcagcaa acggcaaatt
    48781 ggtcaaatca gctacacacc aaaatgcttc ggcagattgt tctgtagctg cgtagactcc
    48841 ttcttttgaa atatcaaatc caacagcatt ttgaatcttt ttcaattttg aaagttcatt
    48901 caagaagcta ccttctccgc agccaacatc taatagcgag acttcttcag gtaaatgctg
    48961 cgcaatagtc tcaataacgg gctgatacat gccagattgg atcattctac gccttggaac
    49021 gaacattcct tgatcgtatt ctgttttaac cgcatgggat aaaaaataaa gtgttccttt
    49081 tttggaaagg tcaaaacggt gattattttt gcatatcaag ccattatcag acttttttac
    49141 ttcttcttgg caaaccggac agcggaacag ctgatgattt tctgccaaaa actggcgtcc
    49201 tcggtcaatt ttttttaaca tagttccccc tcatttctgt tttatcttac cgttagatgc
    49261 tcactttggc aactgctaaa aatatgatac aatgcctaga agaaagtgaa agataaagga
    49321 gaagattaga tgatcaaaga attttgcgct gaaaactaca catctattcc tctagcaatt
    49381 gctagaggag ccgatcggat cgaattgtgt gacaacttag cggtcggtgg cacaaccccg
    49441 agtactggtg tgatcgaaga aacgttaagt tatgctgggg aaaaagatat ccccgtaatg
    49501 acgattattc gtcctagagg gggcgacttc gtctataacg atatcgaatt gaaaatcatg
    49561 catactgatt tgattgaagc aaagaaatta gggacggatg gcgtagtatt tggttgtctt
    49621 actccttcag gctggctaga cgaagaggcc ttagaactat tgatcgaaac agcagaaggg
    49681 ttgcagatca ccttccatat ggcttttgac agtattcctg aagatcggca attcgaagca
    49741 atcgattggc tggtagaaca tggtgttgac cgtatcttga ctcatggcgg tcctgctgac
    49801 aaaactattg tagaaaattt cccacggctt aaagaattga ttgcctatac agatggccgc
    49861 ttaatcattt tgcctggtgg tggtgtcact gcagagaatg cagcacatgt agctaaagaa
    49921 ttgaacgtaa gcgaagtaca cggaaccaaa atcgtggatc ttcagttata atcttataaa
    49981 caaaaaaaca tttgcaacag gcataatgct tattgcaaat gtttttgttt ttttacataa
    50041 aatagcccat aaataaagaa gcaatattga aataaatcag tacactcgtc aaatcgctca
    50101 aagtcgtgat aaagggaccg cttgctactg ctggatcaaa tcctagacga tccattagca
    50161 tcgggattaa actacctgca agattcgcca ctgtgattgc aaatagcata gccatcccta
    50221 tgacaaaccc caagataaaa ttatgtttcc aaaaacctac gattagaaaa atcgtgatcc
    50281 ccgtaacagc tcctgtgaca acgccggtca agacttcagc caaaacaagt cgaacaaatc
    50341 catgatcttt ctcatcagaa atcgcaagtc gacgaacagc cacagctagc gactgggttc
    50401 cagcattacc ggcagttcct gtaatcagcg aaatgaagac tgctaaaata ctcgcttcac
    50461 ttaccaagtc ttcatagtga ctgattaaag aagcggtcga catacctaaa aacaacagag
    50521 tgatcagcca aggcaaacgt cgagacgctg cctttactgg attttcatta atttcttcca
    50581 cattgacccc ggcaagtcct gaataatcgc tagcagcttc atcatcaata acgtcgatga
    50641 tatcatcgac tgtgacgatt cctaacaaat gatcatcata atcggtaaca ggaagagcta
    50701 agaagtcata atctctaaat gtttgtgcca catcttcttg gtcgtcgcct acatgaactg
    50761 aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc gtcatcattc acgattaggt
    50821 cgcgcagaga aataacccct actagctgac cttcttgatt aacgacatag atgtaataga
    50881 ttgtttcagc tacatctgct tcgttcttca gcacgtacat cgcagagcga acagtttgat
    50941 tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc agccgtttca tcctcgtaat
    51001 ggagaagttc cttgatttca ctggcatcgt ctgccgacat caaacttaaa tatttagcaa
    51061 tttgcttttt atccagcgta ttaagcaagt caacagcatt atctgtatac atctcagaaa
    51121 gcatatcagc tgcatagcta ggacgcattt catctaggta ttctttcata tgttcgttgt
    51181 cttcttcaat cacatcgaac atatccgcta attctttagg cgacagatag gaatagatca
    51241 gctgcctgtt ctcttctgta agagattggt agaattgccc ctgctcatag atatgcatct
    51301 ctaaaaaact gtttctgaat gcctgaatat tattttccct taaatcttga gtcaactggg
    51361 caaatctttc ctctaattct tgattttcat ccaaaccaat ctcacctcgc cttattcttc
    51421 aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc tgcttgaact ccagcaactc
    51481 ttctgtaaat ggatggtaaa aacgcagatc ataacaatga agggcttgtc tttttattcc
    51541 cagatccatt cgcccgccat acatatcatc accgagcagg gcacagccaa ttgcggaaaa
    51601 atgaactctt atttgatgtg tccttcctgt atgcagctgg atatcaacta acgcgatttc
    51661 tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa cgtccttcat cagagaccat
    51721 tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa tcgatatgtc catgtgccga
    51781 taaatctgaa agccttccac tgactaaggc ctgatatttt ttcacaaact tcttttcacg
    51841 aagttgcata tctaattttg cgtgcgcaaa tccatgcttt gcaaaaagca tcaaaccaga
    51901 tgtatcccga tctagtcgcg tcaccacatg gattacttgg ttttcgtaac cttgtttttt
    51961 ataataagcc tttacacgat tagccattgt cccatttgga tggtattggg caggaataga
    52021 cgaaacgcct gctggtttat ttactaccag aacgtgttca tcttcataca cgatatcgat
    52081 cggtgtttca tccaaaagta cggtttcatg ttctccttca gcaggaatca caatggttac
    52141 cttatcatcc ttagctagag aaaacaagac attttctact tgatcattaa ctttgatttg
    52201 accgccttga aattttattt tcgctaacag accctttgag atcccttttt cttttaaaaa
    52261 atattttact tgctgagaat gatctttctc ataatcccat gtaaactgca tatcaatcct
    52321 cactaataaa ggcatctttc acacgatgcc aaaaatgcat atgtcgataa gaagcaaaat
    52381 ggatccgttc ttctgctatt cgataataaa ttgagcggat atttgctttt gcgacattca
    52441 attgatcaac cgttacaaga taatcttctg tattttccaa ttttatttct agccattcat
    52501 catgggcaat cacgatcgga gaacccaaag tacgaaacac tcgattattc agtgaagcaa
    52561 tctctgccaa ctgaaaggca ttgatactag gatggatcac agcaccgccg acacttttat
    52621 tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc atctcctcta aaactttcaa
    52681 acagctcgtt tttgatgtat acatcggcta ccatcgtacg attggcccgt ttaatcgttg
    52741 attcattcag tgctaaaaaa tgctggtccg gtgtttcatc caaataactg atccgaacat
    52801 ccagcaaggg ataactaaca ctttgttccc ggttcgttcg taagctgtct actagttctt
    52861 ccagctcgta atcccgccaa tccgtgtaaa accctaaatg acctgtatgc acgcccaaga
    52921 aacgcacatc attcagacaa tgactgtagt gatggaacgc cgacaataat gtgccgtctc
    52981 cgccgactga gatgaccaat tctggctcat gatcatctcg ttgaatccct gcttgttcta
    53041 aaagtgcgtt caaacgttct gtcacttcta tagtctttgc ttctttatta tgaacgatcg
    53101 ctactttcat cccctgcctc ctctctttcg ctttctttct tcaaaagcgt tcgatagtag
    53161 acatcatctg attttcctct tccatgggag aataaccgct gtgcttcttg gatttcttca
    53221 cggatactgg acatctcttc atccaactga taggcagctt ctgccgcacg ctgaagtcgt
    53281 tcactcattt cttctggaaa aacgccttga tatttataat tcagcgaatg ctcaatcgtt
    53341 gcccagaaat tcatcgcaag agtccgaatc tggatttccg ccaaaatttt cttctcaccg
    53401 ctgatcagct gtacaggata ctcgatcacc agatgataag aacggtagcc acttgctttt
    53461 ttatttgtga tgtaatctcg ttcttggaga actttcatgt cttttctttt acggatgatc
    53521 tcaacaactt gatggatatc ttctacaaac tgacacatga tccttaaccc ggcaatatca
    53581 gacatttctt cttctaaacg tcccattgga atatgccgta attgtgattt tgttacaata
    53641 ctatcgactg gcttgactct cccagtgaca aattcaatcg ggacatgtcg attttgttca
    53701 cggaactgtt tgcggatccc ccgcagtttc actttcaatt ctgatacagt ttgttcatat
    53761 ggtgctaaaa atgcttccca atctcgttcc atagctgata cctctacttc attaacattc
    53821 attgtttatt ctaccatata tgcaaagttt tttcgtactc ttgatttatg cattgaccaa
    53881 aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc aaatgacaca aatgcttgaa
    53941 atcgaatata aatctatgct gacaaagcaa gagtacgaga tattcatcgc tcactatcaa
    54001 ttaacagata aagatttccg tgtacagact aatatttatt tcgataccgt tgatgaacag
    54061 ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg gtcatcaagc tgaatataca
    54121 ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg atacctttat aacagaagaa
    54181 gcagaagcat ttatcttcga aaaacgtctg cctcgttcag gcgcagtttt tcaaaaactt
    54241 tctgatttga atatcgatcc tgcttcatta atccaaaccg gaaagcttac aacaaaacgg
    54301 gctgaattcc ctattgaaga aggactttta gctattgatg aaagctgggg agacaatcta
    54361 cacgattttg aactggaact agaggtaagg ggatgcatct gtgggaaaaa ttgcttttat
    54421 aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat
    54481 gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta
    54541 cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat
    54601 tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg
    54661 tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa
    54721 agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat
    54781 gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac
    54841 ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg
    54901 ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact
    54961 tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca
    55021 gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat
    55081 cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt
    55141 agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga
    55201 taactcatat caagttttta atggtgaaca tttacataca aatgattctc agttccattc
    55261 aagaactagc catttacact tgctttctaa ttaaaaaaat acgtcgactc ccttacaaga
    55321 gagccgacgt atttttttcg tttctattcg agaaaatcaa aaatagaaag ccttataaac
    55381 gatcgatcaa ttcttctaat tcatttagac gttgttcaaa catcgacatt gcatcttcaa
    55441 tatatgcagc ctgtgtcata tcaactcctg cttttttcat tacttccaca ggataatcgc
    55501 tgttacctgc ttttaggtaa gccaagtagt tctctagtgc ctctggttcc tggtttagta
    55561 ttttcttcgc cagtgctgaa gcagcagaaa agccagtcga gtattggaaa acatagtaat
    55621 tataataaaa atgcggtatc cgtgaccatt cgaatttaat ttctgggtct tcttcgactg
    55681 ctggaccata atattttgca ttcaatttac cataactatc acttaggtat tcactagtca
    55741 atggcacacc tttttcatct tccgtatgca tgaaatgctc gaattcagcg aattgtgtct
    55801 ggcggaaaac tgttccttta aacccatcca agtaatggtt gagtacataa gcccgtacac
    55861 gagggtcttt ttctgtttcc aataaatact ccgttaggat attttcattc gttgtcgaag
    55921 caatttctgc taaaaagatg gagtagtcgc cgtacacata aggctggttc gaacgagtga
    55981 aataactatg aacactatgt cccatttcgt ggacaagcgt aaatagctga tccagcgtat
    56041 catgccaatt caataaaata tatggatttg tgtcatagct tcccgaagaa taagcaccgc
    56101 ttcgtttccc tttattttcg acaacatcga tccaacgttc agagaatgct ttttctacga
    56161 tggccatgta ttcttcaccc attggtttca gtgcttctaa agctttttct tttgcttctt
    56221 cgtacgtaaa ggtaattgga gcttccccta agaccggtgt ataaagatca tacatgtgca
    56281 gtttttccac ttctaataaa cgtttccgta attccatgta tcgatgtaac aaaggcaaat
    56341 gcttgtttac cacgtctacc aaagtatcgt atacactttc aggaatatga ttattgctca
    56401 aagacgcttc tctggcagag ctgtaattac ggactttcgc tttaaaatta tgtcctttta
    56461 tatgtgtgcc taaagtagaa gcaaatgtat ttctaaattg ttcgtaaaca ctgtacaatc
    56521 ccttaaatgc tgcttcgcgc acccttcgat ccgtgctttc tagcaactga ccatacacgc
    56581 catgagataa ttggactatt tcaccatttt ctccttcaat cgttggaaaa actagatctg
    56641 cattattcaa aacagcaaat gtatcacttg aagcatcaaa gatttcacct gctccagcaa
    56701 gaagagattc ctgctcagca gataagacat gggctcgatt atctactatt tgctggatat
    56761 aatggcgata gacttccaat tttggttctt ctttgaaata ttgccaaatc tggtcatctg
    56821 acaattgcaa tatttctggt tcaaaccagg aaacggcttc actaactttc gaaaacagac
    56881 tgcttgctct tgcataaagc gcttggtaat ctgtatttcc agtgtcttga tcgtttttaa
    56941 ggtgcgcata tacataaatg acttcagttt ggcgataaac cctcaatacg aattcaatcg
    57001 catttaaaaa ttgagaagcg ccttgatcaa gtgttccttt gtgtttttca gattgtttta
    57061 actcttctga taattccaaa tatttctcat caaactcttg gtcgcttgaa aagatcttgg
    57121 tcaagtccca agttaaattt tcaggcaatt cttctcgttt tggcaactgc tttacttcca
    57181 tgaaatccac tccttttctt cccttagctc tatctatcaa aatagatcat agtctaacat
    57241 ccacatgtca cagcatctaa cttttctact cattcatgtg tctgcttcca tcaattttag
    57301 cataactaaa tcttgtttta catagaaaac cgattcctag cctattttta tacttactat
    57361 tttttcaaag aagacaagtg tatgcattcg ttaaaaaact gctgatagac catctcttca
    57421 tcaatcatag gaaaaagcca ttccctttta tgttctttta tttggcaaag ccatgagttg
    57481 aaatcaggaa ctttctggtt ttccaaggct tcttcgtaca acattcgata aagaaaaacc
    57541 tgttcttgta aataaaagaa aaaccgactt ttttgataga tccacgatga tagatgcaaa
    57601 agatgtttat gacgcaagta gcattgttct tgtatggata ctgtattctt ttgcttgtga
    57661 ataagttggc gtgtcagcca tttcttccca tcttcactca ctttttctat ttttttcatt
    57721 tggttcattt taatttcgtt actagtgaat atttctttaa ggtccctact agaaaaagtc
    57781 cagcagctac tgcaaaagga aatatgccct gatacagtct ctgtaatatg atgatacaaa
    57841 taaagctttt gtttcttgag atcagctttc cagcaatgca ctcctctttt tctattatag
    57901 tagcagcaac ttttttctat caatgagaat tgcgattgtc caagaaaaga attaccaagt
    57961 atccaccata cttggtaact gttattcaaa taattttggg ttctttcaat aaatctctgg
    58021 tgagacagtc tagaacattg gatttctatt gctagattcg ccaacaaaat atccggcctt
    58081 tgccgcaacc tcggcaaata ggcttccaaa aatactggtt ctactgactg tcccagccaa
    58141 tccatgaagc attcctttaa ctgtaagtgc tcttttgatt ctctttcact aaagaccgaa
    58201 cagttatctg atttttgatg ggcaaaatga gcacattttt tctttccctt tttcaaaatc
    58261 actctttttc gacagccacg acaataatat ttctttcctt tttctgcatg tttagcaaaa
    58321 cagcacttat tatattcatt cattgcaagc agcatttttc tccctctgaa cttatttacg
    58381 tctaagaatt tatttacacg aaaaaagagg gcgtgaaaaa gctattctgc atcaagtaat
    58441 aagaacagct aaacaaaaat agttcccaat atttttcgtt tggttgggct tgttaccgca
    58501 gatgcaagct tttgaacaca tttatacgaa aaaaagactg cgacacaatt tttgtcacag
    58561 tctcttacta taatgctgat ttatacaaaa taaaatttcg ttcgctcgat agcattacgc
    58621 gccatcagac attgtccata ttcattcaat gtttctggtg tacgatcgct cttttcagca
    58681 aactccaaaa ttcgagcaat ctgattctct acatccgctt ggttgagatt ttccatccag
    58741 aagtgaacga ctaggtaata gcgctcttcg aataaataca aatcagtcca agcagactct
    58801 agttcgacat ctgttgcaag ttggatcact tgttcaaaat cttcaaaagc aaaagttgct
    58861 tgtctttcct gttcttctag ggcgcttcca gataattctt cctccggatc gctgccaata
    58921 atctgctgac gaagaaagtc actcacatct tctgaatcaa cagtagaatc ttctgattga
    58981 gaaatttctt catccggaag atttttactg ataaacagtt ctaatccgtc tccttttggc
    59041 aaaacttgga aagtgacagc ttcgcttcct cggaactcct cttcgatatc aacttcttcc
    59101 aaaatactat aaaagaaatt ttcgatttct ttgtgattgc caagtaaatc caaaaatgaa
    59161 acgccccgtt cctctaaatc ttcatgacca attagtacac ggatcgtatt ttcattaata
    59221 tgttccattt ccattttgct acacctcact ttgccacaat gtttaatata gattcattgt
    59281 agcgaaatca gcttaaatgt aaagaaaaaa aatctcgctg ttaagcgaat ttaataaaac
    59341 cagcggattt ttcaaccttt caagctatct tatttaacta taacattttt ggcagacgaa
    59401 tactatatag ccgacttttc agctaatttt ccctctctat ttttcgtgca atcatcatct
    59461 tctactacct ttttatacat ctaatcaaga atactttttg taaaaacaaa aaaacttgga
    59521 aaaactatct ttgtaatagt
//
LOCUS       DAZKPO010000006        53721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000006 DAZKPO010000000
VERSION     DAZKPO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.006"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..840
                     /locus_tag="ACG8NZ_000379"
     CDS             160..840
                     /locus_tag="ACG8NZ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0538082.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ACG8NZ_000380"
     CDS             930..1964
                     /locus_tag="ACG8NZ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0538083.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ACG8NZ_000381"
     CDS             1978..2313
                     /locus_tag="ACG8NZ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538084.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ACG8NZ_000382"
     CDS             2297..4777
                     /locus_tag="ACG8NZ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538085.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ACG8NZ_000383"
     CDS             4833..6392
                     /locus_tag="ACG8NZ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538086.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ACG8NZ_000384"
     CDS             6389..7753
                     /locus_tag="ACG8NZ_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0538087.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ACG8NZ_000385"
     CDS             7924..8994
                     /locus_tag="ACG8NZ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538088.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ACG8NZ_000386"
     CDS             9009..9677
                     /locus_tag="ACG8NZ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538089.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ACG8NZ_000387"
     CDS             9697..10275
                     /locus_tag="ACG8NZ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0538090.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ACG8NZ_000388"
     CDS             10676..11587
                     /locus_tag="ACG8NZ_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0538091.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG8NZ_000389"
     CDS             complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG8NZ_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0538092.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ACG8NZ_000390"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ACG8NZ_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0538093.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ACG8NZ_000391"
     CDS             13579..14127
                     /locus_tag="ACG8NZ_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0538094.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ACG8NZ_000392"
     CDS             14242..16602
                     /locus_tag="ACG8NZ_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0538095.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ACG8NZ_000393"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ACG8NZ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0538096.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG8NZ_000394"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG8NZ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0538097.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ACG8NZ_000395"
     CDS             19108..20007
                     /locus_tag="ACG8NZ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538098.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ACG8NZ_000396"
     CDS             20000..21262
                     /locus_tag="ACG8NZ_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538099.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ACG8NZ_000397"
     CDS             21423..23321
                     /locus_tag="ACG8NZ_000397"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0538100.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ACG8NZ_000398"
     CDS             complement(23651..24370)
                     /locus_tag="ACG8NZ_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0538101.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24560..24607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24707..25039
                     /locus_tag="ACG8NZ_000399"
     CDS             24707..25039
                     /locus_tag="ACG8NZ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538102.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ACG8NZ_000400"
     CDS             25026..27029
                     /locus_tag="ACG8NZ_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0538103.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ACG8NZ_000401"
     CDS             27048..27518
                     /locus_tag="ACG8NZ_000401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0538104.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ACG8NZ_000402"
     CDS             27539..28123
                     /locus_tag="ACG8NZ_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0538105.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ACG8NZ_000403"
     CDS             28138..29124
                     /locus_tag="ACG8NZ_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0538106.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ACG8NZ_000404"
     CDS             29114..29425
                     /locus_tag="ACG8NZ_000404"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0538107.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ACG8NZ_000405"
     CDS             29447..31228
                     /locus_tag="ACG8NZ_000405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0538108.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ACG8NZ_000406"
     CDS             31221..32597
                     /locus_tag="ACG8NZ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0538109.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ACG8NZ_000407"
     CDS             32604..33239
                     /locus_tag="ACG8NZ_000407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0538110.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ACG8NZ_000408"
     CDS             33269..34624
                     /locus_tag="ACG8NZ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0538111.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="ACG8NZ_000409"
     CDS             complement(34816..36210)
                     /locus_tag="ACG8NZ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0538112.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="ACG8NZ_000410"
     CDS             36302..37273
                     /locus_tag="ACG8NZ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0538113.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="ACG8NZ_000411"
     CDS             37270..38052
                     /locus_tag="ACG8NZ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0538114.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="ACG8NZ_000412"
     CDS             complement(38221..38592)
                     /locus_tag="ACG8NZ_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0538115.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="ACG8NZ_000413"
     CDS             38726..39169
                     /locus_tag="ACG8NZ_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0538116.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG8NZ_000414"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG8NZ_000414"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0538117.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="ACG8NZ_000415"
     CDS             complement(40040..41407)
                     /locus_tag="ACG8NZ_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0538118.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="ACG8NZ_000416"
     CDS             complement(41391..42221)
                     /locus_tag="ACG8NZ_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0538119.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="ACG8NZ_000417"
     CDS             42384..42821
                     /locus_tag="ACG8NZ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0538120.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG8NZ_000418"
     CDS             42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG8NZ_000418"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0538121.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="ACG8NZ_000419"
     CDS             43706..44212
                     /locus_tag="ACG8NZ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0538122.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="ACG8NZ_000420"
     CDS             44229..45128
                     /locus_tag="ACG8NZ_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0538123.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="ACG8NZ_000421"
     CDS             45215..46099
                     /locus_tag="ACG8NZ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0538124.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="ACG8NZ_000422"
     tRNA            complement(46291..46362)
                     /locus_tag="ACG8NZ_000422"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG8NZ_000423"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG8NZ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0538125.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47905..48996
                     /locus_tag="ACG8NZ_000424"
     CDS             47905..48996
                     /locus_tag="ACG8NZ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0538126.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="ACG8NZ_000425"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="ACG8NZ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0538127.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /gene="pgk"
                     /locus_tag="ACG8NZ_000426"
     CDS             50148..51338
                     /gene="pgk"
                     /locus_tag="ACG8NZ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0538128.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG8NZ_000427"
     CDS             51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG8NZ_000427"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0538129.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="ACG8NZ_000428"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="ACG8NZ_000428"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0538130.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17827 a         9355 c        11351 g        15188 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttagttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgacttgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagtt t
//
LOCUS       DAZKPO010000007        51747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000007 DAZKPO010000000
VERSION     DAZKPO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.007"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..976)
                     /locus_tag="ACG8NZ_000429"
     CDS             complement(<1..976)
                     /locus_tag="ACG8NZ_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0538131.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
     gene            complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8NZ_000430"
     CDS             complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8NZ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0538132.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8NZ_000431"
     CDS             complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8NZ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0538133.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2753..3391)
                     /locus_tag="ACG8NZ_000432"
     CDS             complement(2753..3391)
                     /locus_tag="ACG8NZ_000432"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0538134.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8NZ_000433"
     CDS             complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8NZ_000433"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0538135.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8NZ_000434"
     CDS             complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8NZ_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0538136.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8NZ_000435"
     CDS             complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8NZ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0538137.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7185..7925)
                     /locus_tag="ACG8NZ_000436"
     CDS             complement(7185..7925)
                     /locus_tag="ACG8NZ_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0538138.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8NZ_000437"
     CDS             complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8NZ_000437"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0538139.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8NZ_000438"
     CDS             complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8NZ_000438"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0538140.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8NZ_000439"
     CDS             complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8NZ_000439"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0538141.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8NZ_000440"
     CDS             complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8NZ_000440"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0538142.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8NZ_000441"
     CDS             complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8NZ_000441"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0538143.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8NZ_000442"
     CDS             complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8NZ_000442"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0538144.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14805..15089)
                     /locus_tag="ACG8NZ_000443"
     CDS             complement(14805..15089)
                     /locus_tag="ACG8NZ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538145.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15107..15997)
                     /locus_tag="ACG8NZ_000444"
     CDS             complement(15107..15997)
                     /locus_tag="ACG8NZ_000444"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0538146.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16017..16235)
                     /locus_tag="ACG8NZ_000445"
     CDS             complement(16017..16235)
                     /locus_tag="ACG8NZ_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0538147.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16205..17905)
                     /locus_tag="ACG8NZ_000446"
     CDS             complement(16205..17905)
                     /locus_tag="ACG8NZ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0538148.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18172..18945
                     /locus_tag="ACG8NZ_000447"
     CDS             18172..18945
                     /locus_tag="ACG8NZ_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538149.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8NZ_000448"
     CDS             complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8NZ_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0538150.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19661..19763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19769..20740)
                     /locus_tag="ACG8NZ_000449"
     CDS             complement(19769..20740)
                     /locus_tag="ACG8NZ_000449"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0538151.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8NZ_000450"
     CDS             complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8NZ_000450"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0538152.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8NZ_000451"
     CDS             complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8NZ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0538153.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23695..24519)
                     /locus_tag="ACG8NZ_000452"
     CDS             complement(23695..24519)
                     /locus_tag="ACG8NZ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0538154.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24492..25172)
                     /locus_tag="ACG8NZ_000453"
     CDS             complement(24492..25172)
                     /locus_tag="ACG8NZ_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538155.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25195..26133)
                     /locus_tag="ACG8NZ_000454"
     CDS             complement(25195..26133)
                     /locus_tag="ACG8NZ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0538156.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8NZ_000455"
     CDS             complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8NZ_000455"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0538157.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8NZ_000456"
     CDS             complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8NZ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0538158.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30097..30903
                     /gene="proC"
                     /locus_tag="ACG8NZ_000457"
     CDS             30097..30903
                     /gene="proC"
                     /locus_tag="ACG8NZ_000457"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0538159.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8NZ_000458"
     CDS             complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8NZ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0538160.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8NZ_000459"
     CDS             complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8NZ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0538161.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34107..35348)
                     /locus_tag="ACG8NZ_000460"
     CDS             complement(34107..35348)
                     /locus_tag="ACG8NZ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0538162.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8NZ_000461"
     CDS             complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8NZ_000461"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0538163.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36375..37298)
                     /locus_tag="ACG8NZ_000462"
     CDS             complement(36375..37298)
                     /locus_tag="ACG8NZ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0538164.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38037..39323)
                     /locus_tag="ACG8NZ_000463"
     CDS             complement(38037..39323)
                     /locus_tag="ACG8NZ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0538165.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39316..40584)
                     /locus_tag="ACG8NZ_000464"
     CDS             complement(39316..40584)
                     /locus_tag="ACG8NZ_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0538166.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8NZ_000465"
     CDS             40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8NZ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0538167.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41356..41802)
                     /locus_tag="ACG8NZ_000466"
     CDS             complement(41356..41802)
                     /locus_tag="ACG8NZ_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0538168.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41895..42140)
                     /locus_tag="ACG8NZ_000467"
     CDS             complement(41895..42140)
                     /locus_tag="ACG8NZ_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0538169.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42255..42833)
                     /locus_tag="ACG8NZ_000468"
     CDS             complement(42255..42833)
                     /locus_tag="ACG8NZ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0538170.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42910..43440)
                     /locus_tag="ACG8NZ_000469"
     CDS             complement(42910..43440)
                     /locus_tag="ACG8NZ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0538171.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8NZ_000470"
     CDS             complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8NZ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0538172.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44297..44692)
                     /locus_tag="ACG8NZ_000471"
     CDS             complement(44297..44692)
                     /locus_tag="ACG8NZ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0538173.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8NZ_000472"
     CDS             complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8NZ_000472"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0538174.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8NZ_000473"
     CDS             complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8NZ_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0538175.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(46720..46959)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8NZ_000474"
     CDS             complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8NZ_000474"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0538176.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48586..49743)
                     /locus_tag="ACG8NZ_000475"
     CDS             complement(48586..49743)
                     /locus_tag="ACG8NZ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0538177.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8NZ_000476"
     CDS             complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8NZ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0538178.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51210..51737)
                     /locus_tag="ACG8NZ_000477"
     CDS             complement(51210..51737)
                     /locus_tag="ACG8NZ_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0538179.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        14069 a        11268 c         9225 g        17185 t
ORIGIN      
        1 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
       61 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
      121 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
      181 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
      241 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
      301 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
      361 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
      421 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
      481 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
      541 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
      601 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
      661 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
      721 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
      781 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
      841 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
      901 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
      961 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
     1021 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
     1081 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
     1141 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
     1201 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
     1261 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
     1321 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
     1381 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
     1441 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
     1501 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
     1561 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
     1621 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
     1681 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
     1741 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
     1801 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
     1861 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
     1921 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
     1981 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
     2041 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
     2101 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
     2161 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
     2221 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
     2281 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
     2341 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaatc
     2401 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
     2461 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
     2521 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
     2581 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
     2641 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
     2701 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
     2761 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
     2821 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
     2881 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
     2941 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
     3001 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
     3061 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
     3121 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
     3181 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
     3241 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
     3301 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
     3361 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
     3421 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
     3481 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
     3541 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
     3601 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
     3661 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
     3721 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
     3781 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
     3841 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
     3901 ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg tggatgtaat
     3961 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
     4021 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
     4081 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
     4141 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
     4201 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
     4261 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
     4321 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
     4381 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
     4441 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
     4501 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
     4561 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
     4621 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
     4681 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
     4741 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
     4801 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
     4861 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
     4921 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
     4981 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
     5041 tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg
     5101 atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg aacttgtgga
     5161 gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg agctgctaga
     5221 actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt tagagtaaat
     5281 tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg tacgaatcac
     5341 tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat attctgcacc
     5401 gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat cattcaattt
     5461 aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta caacttcacc
     5521 cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat agtcataagt
     5581 agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa cagtttcgta
     5641 gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt
     5701 aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg aaatgatcat
     5761 tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag cagaaaaccc
     5821 taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat catctaattt
     5881 gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct tgactgtcgt
     5941 tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat ctgcaatttc
     6001 ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag cagaagcttc
     6061 agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag caaagaatac
     6121 agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc attttttctg
     6181 tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt cgttttctgg
     6241 cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat cttccgtaag
     6301 gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt cttcgtttgc
     6361 acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg acttataacg
     6421 gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt
     6481 tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag caattgttac
     6541 agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga tgatccctaa
     6601 agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag gggacagata
     6661 atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata aagcgatagc
     6721 aatcccgaaa tccgtgattt ttacaacacc ttcattatcg atcagtacgt tttggggttt
     6781 caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa ggatctgctg
     6841 cataatattt actgctgttt cataaggaac aggataatgt gtttggatat atcgtttcaa
     6901 gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt caccaacatc
     6961 gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt ctctctgaaa
     7021 tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa ctgctacatc
     7081 acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc taccgatatt
     7141 tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac actgtcctcc
     7201 ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt ggcacgatca
     7261 atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt tcctatttct
     7321 tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc ttttagttgc
     7381 caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga acgtgttaaa
     7441 atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga tttgactagt
     7501 tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg aatcaaataa
     7561 gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg
     7621 attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata aatcagcata
     7681 ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc aacagcttct
     7741 tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat gtctccggca
     7801 cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg gctggtaaat
     7861 acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg atattcaatc
     7921 tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa
     7981 aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag cataggattg
     8041 gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga aacaacgtct
     8101 tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga agactttaaa
     8161 gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc taattcatcc
     8221 ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc tgaacaaggc
     8281 gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac ttctctagca
     8341 tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt atcttcaatg
     8401 agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca tgacggatct
     8461 aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc taagacatga
     8521 tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc ttggatcgtc
     8581 atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc tactataccg
     8641 tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac agtgatggcc
     8701 tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg ctgatatagc
     8761 gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc
     8821 catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat gccttgcaga
     8881 tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt ccctgtccca
     8941 ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg tgcagggact
     9001 ttatctaaaa attctaattg gtatacagaa agataaagaa gcatcttgac ccagtcatcc
     9061 actttttttg ctttagcgat caatggtgca acgtaaaatt ccaatagtcg ctgatggcta
     9121 atcgtaccat agatcaattc tgtaaataaa cccacatctt tttgatttag ctcgctttgt
     9181 ttgatcatct cattcaacaa aagattcgag taggcgccac ccttgttcac tcgctctaat
     9241 gtcagtaatg ccatatatcg tactgatttt ttcatttttt tagggatttt tttcattatt
     9301 tcacctgctg accaatctga acttgctgac cgcttccgtt aaggaaatca tgtacggcct
     9361 gttttccttt accagctggc tgtaattcat tgatggctaa gacggtctgt ttaccgcaag
     9421 caatccatag attttttttg tctttccgga tgatcgtacc aggtgctttg tctgtagttt
     9481 ctgccaatac ttcaaccgat aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa
     9541 ttggccaagg acgcattccg cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat
     9601 cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag
     9661 gtactggttt ttgtccatcc aaaatattgg gcaatgtttc tagcaatagc tttcttccta
     9721 aaaggctaag cttttcaaac atcgtcccta catcatcttg tttagtgatt ggtatgcttt
     9781 cttgcgcata gattccgcct gcatccattt ttttgatcat ttccatgatc gttacaccag
     9841 tttctttctc cccattcatg atggcataat gaacaggcgc acctccccga tatttaggaa
     9901 gtaaagaagc atggacattg attgccccta attttggtac ttgcaataac ttttcaggca
     9961 agaattggcc aaatgccgca gtgacgatca catcaggcac caattcaatg atttcttcca
    10021 tttctggcga accggagatt ttctctggct gcagcacacg gataccgtgc ttcagagccg
    10081 ctttttttac cggtgtcggc gtaattactc gtttccgccc aactggacgg tcgggctgtg
    10141 ttaccactgc ctggatctcg taacctgatt ctatcagccc ttcaagaatc ggcacagaaa
    10201 aagcaggtgt gcccataaat actagcttag tcatcaagat gttcctccat atattcttca
    10261 agttcttctt ccgggatctg ctgtatcatt ttctcaatga acaagatacc gtctaagtga
    10321 tcgatttcat gctggaaggc acgggctaaa tagccaaaag ccgtcacttc tatctcttct
    10381 ccgtctcgat cataataacg cacggtcact tcatctgctc gcttgaccgt tccatagaca
    10441 tgcggaatac tgagacaacc ttctacgtca aggctttcac cttttgcttc gatgatttct
    10501 gggttaatca gttcaaattt ctcgccttca tctacttcga tcacagcaat tctcttgttt
    10561 tgtcctactt gaggagcagc gatccctatt ccgtcattcg caatcattgt ctcatataaa
    10621 ttgtctaata aagcgattgt ctcgtccgtg attacatcga ttggctgagc agttcttttt
    10681 aatttatcat tagggtggat caatatagga tagcgcataa tttggccctt tcttaaataa
    10741 agtttaatgg ttcgttgtca attgccacaa aaagaccttg tcgctgttct ttctggcttc
    10801 cattcaaaat ttcatccaaa acatgaggaa gattcggttc atgtttatat ttgatgatta
    10861 gttgataata atatcggttt ttcactctca aaatcatgct gggcgttggc cctaatagca
    10921 agctttgagg gcttaacact tcttttagct gattggcaat ctgaaagatt ttctttgccg
    10981 ctacttgttc ttcaggatgg ctggcggtta ttttcacagt aaagtaataa ggaggatagc
    11041 ctccacgatg ccgtacatgc atttcctgct gataaaactg ctcataattt tgttttttag
    11101 ccaattctat ggcgtaatgt tgaggattga atgtttggat cacgacttcg cctgcttttt
    11161 ccgctcgccc tgctcgtcct gaaacttggg taagcaattg gaatgtatgc tcactagaac
    11221 gaaaatccgg taaattcagg gaggtatcag cattcaacac cccaactaat gtcacttctg
    11281 gaaagtccaa acctttagca atcatttgtg tccctaataa tatatctgct tcttttgcac
    11341 cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg agtggtatct acatccatac
    11401 gcaaaatcct agcttgcgga taaagttctc ggagttcctc ttctactttt tgagtacctg
    11461 ttccatagta gcggatttta ttgccgccac aattaggaca gcgattggga ataggttctt
    11521 catgtccgca ataatggcag cgcattgaac gtgtatccat atgcagtgta agagagatat
    11581 cgcaattagg acatggaaga acaaatccac agtccctaca catcacaaaa gacgaatatc
    11641 ctcgacgatt gagcagtagg acagtttgtt cttttttgtc taatcgattt tgtatttttt
    11701 cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg aaactcttca cgcatatcta
    11761 cgatctcaat agctggaagc tgtgcagctg ctttggctcg gtggtttaac tgcaataact
    11821 gataaacgcc tttttgagct cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa
    11881 ctattgggca atgatgatat ttactgcgcc aaattgctag atctctcgca tgataacgcg
    11941 gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc gataatgatc agcccgatat
    12001 ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac tacttgcgct tcttcccgct
    12061 caatcttgcg ccattcatca tatttttcac cttgggaaag tccgctatgt aagaccgcaa
    12121 ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg cggagtcaat gcgatttcag
    12181 gcactaacat aatagccgtt ttattttctg acaaaacatc agcgattgcc tgaagatata
    12241 cttctgtttt cccgctgcca gtgattccct ccagcaaaaa gacctgatct ttttgctgct
    12301 gacctgctgt gatgatttgt tcgacagcat ttttttgttc tgcattcagt tccaatgccg
    12361 tcgtttggtc aaatactcgg tctttataag ggtcgcgata acgttctgat tctataaact
    12421 ccagccagcc cttttctttt ccttgattca agacagcagt actgattcct ttatttttga
    12481 aataagcaat tggttcttct tggctcagtt cagataaaca caaaagaagt tctttttgtt
    12541 tcttggcatt ttgccgtaat tttgcccatt cttcttcgat ttgcttttca gttaacaaag
    12601 aacggaccaa acgaatcgtt tttactttat ttcgtgtatg gacttcatat ttgatatcta
    12661 ctaattgctc ttggcgccag cgaatcaatt gagaaaggct gccttcttct tctgcttcct
    12721 tccaggaaat cacttctcgc tgatcaaaca atacctgaac ttctggtgtg tctgccaaag
    12781 gatagatcaa tttatcatat tctgctttca tcacactcgg aagcatcgtt tgtagacaag
    12841 tgattttgaa tgcgtaagta atttttttca tataatcagc taatgaaagt aattcttcat
    12901 tgaccaccgg agcaaggtct agcaaacgaa taactgcctt caaagatgga ttagctgatt
    12961 cttctgattt ttggatagcc ataacaaatc cttggacatg acgattgcca ttaccaaaag
    13021 ggacttctac tcgcatcccg acttgcaaag ctgtttccat ttcagaggga accaaataag
    13081 tgaatggctg atcggtctgc atagtaggta cgtccacaat gacttcagct gttaatgtca
    13141 tggttcgttt cctccttttt ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat
    13201 tttactagag atcgatggat ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag
    13261 cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca cagccttttc taccagacaa
    13321 taagtggcga atgtttgaaa aggaaacagc tgggtttgct tttcttttta gatgttcgct
    13381 ctatttttct ttattacatt ttttgctctt cacggatacg tgattccaat tcgtgttgtt
    13441 cacgctcttt ttgtgcttga cgttcctctt cttccatctt caaacgttca cgttttaatt
    13501 caggatgtgg atcattgact acatttcctg catcgatttc ttctaaagct tgaccgacgc
    13561 tttttactga atcaaaagaa tctagagtag gctgtgcact tgcatccaat tcatgcgcgc
    13621 gtttacttgc taaaatcact agagaatatt ttgaatttac acgatctaat agtgagtcaa
    13681 ttgaaggttt taacatcata agactacatc tcctttaaca ttttaatata tttaccgatt
    13741 acgcgatcta ctcgaaaatg ttcactggca ataatgtttt tgattcgttc aactgctagc
    13801 ggcacttcat catttacaac agcatagtcg taaagagcca tcatttcgat ttcttctttt
    13861 gcaactttca ttcgtttttc gattacttca tctgcatctg ttcctcggcc tacgatccgt
    13921 gattttaatt ccgctaaatc tggcggagtc aaaaagatga atacaccatc gggcactttt
    13981 tctttgactt gttttgctcc ttggacttca atctccaaga aaacatcttt tccttcatcc
    14041 aatgtcttgt tgacatatga caaaggagtt ccataataat tcccgacata ttctgcgtat
    14101 tccagcatct ctccagcttc gatcattgat tcaaactctt ctttgcttcg gaagaaataa
    14161 tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag tcattgagat cgaatactga
    14221 aaatcattat cctcactctc aaaaattgct tttcgtactg tgccttttcc tacaccggat
    14281 ggaccagaca acacgattaa taatccacgc tctgacatga cgacgtcctt tcaactagtc
    14341 tttttgtact tatcgtttat tttgcacttt ttttctgcaa ctttcaaggt tatctttaat
    14401 tatagctaga aattggtaaa aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct
    14461 gcatcaagta ataagaacag ccaaacataa acagcttctc atgtttttcg tttggttggg
    14521 gttattatcg cagatgcaag cttttgaacg ccgtgtataa gaggtcgtga agaagccgat
    14581 tagtttcgag tatgaagaaa aattttgtga aaatagtttc tcacattttt gcataaattt
    14641 ggcttaatac cgagaaaatg gtttttgaac atcacttatt caaaataaaa aaagattgtg
    14701 acacgaattt tgtcacaacc tttaaatgta ttcctagagc aaagaagcta agcgaagaaa
    14761 tccaattctg tttatttatt gtatttcttt gttcagcatc cttgctaaaa gcccatattg
    14821 gacaaagtat tcactacacc catgatctca tcttttcgag aacgtgcttt ttctgtcgga
    14881 tcttctacct cttctgtgtc aattcgaaca ataaatttcc gattcatcgt cacgtaagca
    14941 ccgtcgttga tcaaaataaa cagttcgttc tcattatagt ctgcacaggc atctttccat
    15001 acgtcaaagc cgtttttttc agaagggata tcccgtttaa ccacagtttt tccacttaat
    15061 tcgtggaacg ttacgtcata atatttcatt atctctacct ctttcgtcat tcaatatttt
    15121 gtacttgttc tctgattttt tctaagatcg tcttagactg gacgacttga tttttaattt
    15181 cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg attcatttct tggatcagga
    15241 aatctaattc ccgacctaca ggacattgtg cttcgataag ttcatctagt ttttgtacat
    15301 ggatcgccag acgatccagt tcctcgtgaa tatctccgcg ttccaacaag atggctagct
    15361 ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa ttcctccagc tttttttgat
    15421 accttatttg aaattccttt tcatagattt ctacaaaact atcgagtttt tttagtactt
    15481 ctttgaattc ttcagtattc tttttcaaca ctaaagctaa agcttgacct tctttttctc
    15541 gactcgcctc aatagaatca agtgcgcttt tcaatgtatc tagagctaac tgtgccaatt
    15601 cttccatgct ctcttccggt ttttcttcta ccgttacgaa gttttcgtcc agcacgaagc
    15661 gttccgccat ccgactaatt gacacttgat tttgactacc gtatcgttgt tgaattccgt
    15721 ctgtcaattg agtgataagt gaatcaatca attcccagtt gatgacgatc tgtttttcgt
    15781 ttttgccaat ataagctaga ttcacaaata cttcgattcg accgcggctt aactgctgtt
    15841 taataagctg acgaaaatcg ttttcaacaa agttcaggat tttaggactt cgtatttgaa
    15901 catctagaaa acgctggttg acacttttta tttcaatatc taactgatac gcttttgttt
    15961 cgcggatggc tttgccaaaa ccagtcatac ttttcattcg acagggtcct tcctcattac
    16021 atattttcta ttgctttttg cagttgttca aactcttcgt tcgtcaatgt caggcctttc
    16081 cccatttttt catggtctgg cgcccaatct cgaagatcga attttggcgg gcgaccattc
    16141 cagctgacta ggttcagttc tttacgccac cccttgttgt tttctgacaa tatagcgatt
    16201 tcttctacga tttcatatga aaattcttgt gccatggttg ttcctccttg agataatata
    16261 aatataggat cagtaatgtg tcaactggtt gcttaagcaa ttcatgctgc aaacgatcct
    16321 tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt cgtgcgctca ttttgtattt
    16381 tttgctggta ttttcgatac cttcgccatt cttcagtact taattgacca atttgtgcca
    16441 ataaatcatc cggcatactt tttatatctt gttggcagat catttgaatc atcatgtcta
    16501 cttgatagat ttcattgggc agtaaaaaat gtttttttgc taaaagttct agcatcgcca
    16561 atccgcatgt ctctggtacg accctgttct tatataaatt acgcagtttt tgccaaaatt
    16621 ttatgctttt gaaaaaagat aattctaact gaatttcttt tgttaagtaa gacaaaaatc
    16681 ctcgccagta tgttcttgca ggtttaacag aatgaaatgt ataccagttc ccatagtaat
    16741 agcctaatcg ttttttcatt tccagatagc ctaattgtaa atttaattgg ttcctttggc
    16801 tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg acatttccaa tggataaaaa
    16861 tgtctggcat acgaattttt ttcgctgggc ctggcccttg cacatctacg acaaatgcct
    16921 ctgttgctcc tttgttaatc gcaatatcaa taggtatttt gttgcgatac ccaccgtctg
    16981 catatttatt ttttccgatc tttcgataag ccatagcggg ataaaatgaa gcagaggcga
    17041 gtatccaatc tggtatttct tctgtttttg tctgttggat atcagtcact acttccgcta
    17101 gttctggcag tttagtagac accgtatata aacgtatttt gggattatgt ttgagtaagg
    17161 acaaatccaa attgtctttg accatctttt ccaatggagc agaagaaacc ccgccttcaa
    17221 ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa gcgttcacga aggtcttcaa
    17281 ctgcagccat ttctggtaac gcaagcactt gtcttgtcga cagctttttc cataaattca
    17341 cagctttttt catatctcct tgtaaaatca atgcgccgtt caacgcacct accgaggtac
    17401 ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc ctgccacaca ccaatctgat
    17461 aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt atgatagacc agctttttat
    17521 ttaattgtgt ggttccttga tagccgtttt tatgaaaaat acgcaaccat tcttctgata
    17581 gaggagattg aaataaaaaa caacagtttt ctttaataaa aaaacgagcg caagcttcca
    17641 tataccgaaa aaattttttc cattcaaaag tttcaccttc aatcaacaga ttattaaaaa
    17701 taacatctcc tctactttta gcaatagtaa aatagacggt atattttttc gatactgctt
    17761 taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc tctactttct tgatgacttt
    17821 gaaaaaatgg aaggggttgt tttgcttgct ggataggcga atacagcgac attttctgtg
    17881 aaaagtaaac tttttttatc tccatcgcgc tctccttttt cttcaagtat accagtacgt
    17941 cataaaaagg tcatgagtct cgtattcgac tttgtagatt ccataagaaa acaattcata
    18001 aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat taattgaaaa tgagagtgtt
    18061 ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa aagaaacaag cttttttaat
    18121 gcaaatgttg taaaataaaa gagattaaga ggaaataaga ggaggaacat gatgactgag
    18181 tcagtttatg tacatattga taccacaagt aatgctgttt taacaaaagg gctgactgct
    18241 actgactttg caaacagtat cgtgcatttt cctcaaaact tgctcttgct tgatccatct
    18301 gcttctgctg gagaatatga atcccataca gggctgaaag taatccgtgg tacagaaaat
    18361 attcagcgtt tcttttcctc ttctcgtaac aggcgtggat tcgatgaatt gaagtggatc
    18421 gactttactg atttaacgat gctgaaagaa ttgacacctt tagaaatttc agaattatta
    18481 tattttggtc atatgaaaac acatcttcat tcgccgtttt tttataaact acagaataat
    18541 tttgtatact tcgatcttaa tgatcagctg aatcggattt attatcgcta tttagatgaa
    18601 ttttatagga tttttgctag taaattaagt tcgatcttgt cagataagat caatgtaaaa
    18661 aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca gtgtggaatt attgaaagaa
    18721 atgcgtaatt tgatgcagga agggatcatt ttttcttttg atcaagttgg tttgatcgat
    18781 ggtaaatatc gtatcccaat ctatgtgatt gaagattata tttggaaatt aaaaggcacc
    18841 cggtatcgag aagaggaaat catcgctact ttaatctact ctgtcaccga tcgacactgg
    18901 caattggtag aagatgccga tctttcttat ctgccattga agtagatcta tttcttatca
    18961 aaaaataaaa aggaaacaag aaactaggac atgattctgt ctcaagtttc ttgcttcttt
    19021 tttatttaca gctcctgtcg tttatctggc acaaaaatcg aaggagtcgt ttgttggatc
    19081 cctaataaaa cgatgatcgt tacaatagct gtactcaacc cgctggcacc attcattacg
    19141 aaagaaaaga gccaaggatt catcccccat agtgcatacg ctccccagaa aaccactcct
    19201 gcaataaaat gccaaaaata tctggaaagt gcgccaatca atgcaccaaa cacgatgctt
    19261 tttctaattt gtttcgtatt atcgtctaca tagccttttt ggacatttga agccatgagc
    19321 cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa tcaacacttg aacgacactt
    19381 aaataataaa cttgtcccaa cggaaaatgt aggatgcccc aaatgaaacc tgccagcatc
    19441 cctgcctttg tccctcttct taacgcaaaa agagtcaatg gaatctgccc taatgaaata
    19501 gaaaaactgc tgccgatctg gatcggaatc aacgacaaca ccatcgctaa cgctgcaaca
    19561 atcgttcctt ctacccaaac tctatttttt cccataaaaa aacttcctcc tttgaataaa
    19621 aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa cagctgttta ttcacaattc
    19681 ctacgctcgc ataaccgata caggtacgaa gggtttagaa atagattcaa tctcagccca
    19741 attggactcc cctttgtgag tttatttatt atttattttt tgtttcaaac aatggactaa
    19801 ctggtttgtt ttcatggata cgtttgattg catctcccat tagtccgcca acacttactt
    19861 catcgatttt gtcgatttta cgatcttctg gaagatagat cgagtctgta acaactagac
    19921 gctcgatagc tgaatcttct attctttgta aagcagggcc ggataatact ggatgtgtac
    19981 atgaagcata cacactagtt gcaccagctt ctttcaaagc attggcagcc aatgtgatcg
    20041 tacctgctgt atcgatcata tcatcgatca gtacacaagt tttgcctttg acttgtccaa
    20101 tgatattcat tacttctgca acatttgctt ttggacggcg tttgtctatg atcgcaatcg
    20161 gagatttcaa aaattccgct aatttacgtg cccgtgtgac accaccatgg tctggtgaaa
    20221 caacgacaac atcatcgcct ttgattcctt tttctaagaa ataattggca ataagtggcg
    20281 cacccatcaa atgatcgact ggaatatcaa agaaaccttg gatttggact gcatgaaggt
    20341 ccaaagtcag cattcttgtt gcaccagctt tttgaatcat gtttgctact aatttcgctg
    20401 tgattggttc ccgtgcacgt gctttacggt cttgacgcgc atagccataa taaggcatga
    20461 ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc aatcatgatc agcaactcca
    20521 tcaagttatc attgactggg ctgctcgttg attgaataac atagacatgc gatccacgaa
    20581 tactttcttc aatgtttact tgaatttctc catcactaaa ttgagtaaca gatgattttc
    20641 ctaactcgac gccgactgct gctgcaattt tctctgctaa tgggcgattg gaatttaacg
    20701 caaaaatttt taatcttggg tcaaagtaat gttttgacat ggggacctcc actttctttt
    20761 tcacaactct agaattctac ctaataaata gtagtcatat tttttaaata aatcaagtga
    20821 ttttgcaaaa gtcttagttt agataaggga gtttttttgc atatccttct ttattgactt
    20881 gtcttgctct cgcaattgcc agcgcatatt ctggaatatt gtctgtaatt gttgacccgg
    20941 cagcaatcga actgttttta gctacctcga caggtccgat gatattcgtg ctagaaccaa
    21001 taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt tccatcatag ttgacgaaga
    21061 caacaccaca tccgacattg atttcttctc ccagcgtagc atcaccaaca taagtcaagt
    21121 gaccgacttt agttcctttg ccgatttgag catttttcac ttccacaaag tttccgatat
    21181 ggacgttttc gccaatttca gcttttggtc gtaaatgtgc ataaggtccc acatctgcat
    21241 gtttcttgac atggctgctt tcaatgactg agttttcaat gacgacgtga tcctcaatcc
    21301 tactgtctac gatttttgaa tgtgacccga tgacacaatc agaaccaatg actgtatttc
    21361 cttggatttg tacacctgct tcgatcaatg tgtctggacc gatttctacg cctgcatcga
    21421 tgtatgtcgt tgctgaatcg acaaagctta cgccattgac catatgcatc tggttaattc
    21481 gctttctcat gatctcattt gctttagcta acgcgatccg atcattgact cccattgatt
    21541 cttcaaaatc atccgtttga taagcagcaa ctgtatggcc ttcttctttt aagatttcga
    21601 tgatatctgt tagatagtat tcaccttgag cattgtttgt tccaactttg cttaacgcat
    21661 cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt gatttcttgt acaagagctt
    21721 cttctggtgt tgcatctttt tgttccacga ttttttctac gatcccaata tggtcacgga
    21781 tgattcgtcc atatcctgtt gggttttctg cttgtgccgt caagatcgtt gcacttgcat
    21841 tttttccttg gtgatattca aaaagattgt tcagcgtttc agtagtcaat aatggtgtat
    21901 cgccactgat aactaacgtt gttccttttt ttccttccaa gaaagaagca gcttgcaaaa
    21961 cagcatgtcc tgttcccaat tgttctgctt gtaacgcata tttactgcgt tctcctagtt
    22021 gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat cacttcatct ggtttcgttt
    22081 cactgacccg attaatgata tgttctacca taggtttacc agaaacggga tgtaaaactt
    22141 tatataattt tgacttcatg cgggtacctt tccctgccgc caaaataatt gcataacgtg
    22201 cgtccaactg cgtcactcct aaatatagtt tgctgatttc agtttatccc agtataccca
    22261 ttttttcaac aaaacaagtg attttctttt atcccagcgt tttctggata aaaaaataga
    22321 tagaccataa cgggcctatc tatttggtaa ccggtgctct cctgccaact cctcggtcac
    22381 aagtcacact actcataaaa catgaaaagc tgttttttga aatcgtttct tgtgacttag
    22441 agttaaacgg taggcttaca acctcttcat taaacgtttt tcaatcagct gaccttcggt
    22501 attagctcaa taaacggcaa aatatgagga gcaatttttc cgtttattga gctaatactc
    22561 aggacaggac gctgatttca caacctctag ataaacggtg ttcaaaagcc agtttctcgg
    22621 tattaagcca aatttatgaa aaaatgtgga aagccatttt cacaaaattt ttcttactac
    22681 tcgaaactaa gcggcttttt cacaacctct tattctgaaa aataatttcc tggcacaacc
    22741 gtaatatttt tcgtttgagt atctacatct ttgacataaa gcagtgacgt ataatcatcg
    22801 atggcgcgat gaccgttgaa ttttgattcg gcaaatacag tgatgccaac aagttctgat
    22861 tcgaattctt caatcaagct cttcattccg ttgactgttc cgccgccttt catgaaatca
    22921 tccactacaa ggacacgtga accacgtttc aaacttctct tagacagttc catcttttca
    22981 attctttcag aagatccaga cacatagttg acacttactg ttgatccttc tgttattttt
    23041 gagtcccggc gtacaataac gaatggcgca ttcagataat aggacacagc ttgggcaata
    23101 ggtacgcctt ttgtggctac agtcattaca gcatcgattt tttcaccaag gtatttagaa
    23161 gcaatgattc ttccaacttg acgcagcagt tcaggttctc ctaacaaatc agagagataa
    23221 acatatcctc ctggaagcag acggtcttgt tctgatagac gttctgctag agattcaata
    23281 tattttttcg cctcttcata tgaaatttca gggatgaaca atacaccacc tgcagcaccc
    23341 gggattgttt ccaaggttcc ataccctcgt tctttaaatg ttttcttgat gattcccaaa
    23401 tcttcactga tagaagattt agcagattca taacgttcgg caaagcttgt taaggaaatc
    23461 aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa ctagacgttc actgcgacgt
    23521 actttcacct attccaactc cttttaagaa attttatcgt tttacattat ataagttttt
    23581 cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata gttcggtatt tattaaaata
    23641 aaaagaagcc tataaaaaat ttttcgtttt tataaggctt cttttcttta taatttagtt
    23701 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
    23761 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
    23821 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
    23881 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
    23941 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
    24001 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
    24061 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
    24121 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
    24181 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
    24241 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
    24301 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
    24361 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
    24421 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
    24481 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
    24541 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
    24601 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
    24661 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
    24721 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
    24781 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
    24841 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
    24901 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
    24961 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
    25021 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
    25081 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
    25141 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
    25201 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
    25261 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
    25321 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
    25381 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
    25441 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
    25501 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
    25561 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
    25621 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
    25681 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
    25741 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
    25801 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
    25861 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
    25921 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
    25981 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
    26041 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
    26101 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
    26161 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
    26221 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
    26281 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
    26341 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
    26401 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
    26461 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
    26521 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
    26581 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
    26641 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
    26701 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
    26761 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
    26821 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
    26881 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
    26941 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
    27001 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
    27061 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
    27121 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
    27181 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
    27241 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
    27301 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
    27361 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
    27421 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
    27481 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
    27541 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
    27601 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
    27661 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
    27721 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
    27781 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
    27841 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
    27901 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
    27961 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
    28021 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
    28081 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
    28141 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
    28201 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
    28261 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
    28321 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
    28381 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
    28441 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
    28501 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
    28561 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
    28621 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
    28681 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
    28741 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
    28801 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
    28861 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
    28921 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
    28981 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
    29041 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
    29101 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
    29161 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
    29221 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
    29281 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
    29341 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
    29401 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
    29461 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
    29521 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
    29581 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
    29641 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
    29701 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
    29761 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
    29821 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
    29881 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
    29941 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
    30001 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
    30061 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
    30121 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
    30181 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
    30241 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
    30301 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
    30361 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
    30421 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
    30481 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
    30541 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
    30601 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
    30661 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
    30721 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
    30781 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
    30841 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
    30901 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
    30961 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
    31021 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
    31081 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
    31141 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
    31201 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
    31261 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
    31321 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
    31381 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
    31441 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
    31501 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
    31561 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
    31621 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
    31681 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
    31741 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
    31801 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
    31861 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
    31921 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
    31981 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
    32041 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
    32101 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
    32161 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
    32221 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
    32281 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
    32341 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
    32401 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
    32461 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
    32521 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
    32581 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
    32641 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
    32701 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
    32761 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
    32821 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
    32881 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
    32941 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
    33001 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
    33061 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
    33121 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
    33181 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
    33241 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
    33301 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
    33361 gcgcgtcctc caggagtgat ttctggattc gcaaacatca cgcctacttt ttcacggatt
    33421 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
    33481 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
    33541 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
    33601 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
    33661 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    33721 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    33781 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    33841 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    33901 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    33961 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    34021 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    34081 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    34141 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    34201 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    34261 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    34321 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    34381 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    34441 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    34501 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    34561 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    34621 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    34681 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    34741 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    34801 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    34861 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    34921 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    34981 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    35041 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    35101 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    35161 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    35221 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    35281 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    35341 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    35401 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    35461 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    35521 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    35581 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    35641 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    35701 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    35761 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    35821 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    35881 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    35941 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    36001 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    36061 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    36121 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    36181 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    36241 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    36301 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    36361 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    36421 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    36481 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    36541 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    36601 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    36661 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    36721 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    36781 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    36841 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    36901 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    36961 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    37021 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    37081 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    37141 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    37201 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    37261 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    37321 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    37381 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    37441 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    37501 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    37561 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    37621 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    37681 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    37741 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    37801 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    37861 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
    37921 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
    37981 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
    38041 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
    38101 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
    38161 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
    38221 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
    38281 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
    38341 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
    38401 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
    38461 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
    38521 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
    38581 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
    38641 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
    38701 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
    38761 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
    38821 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
    38881 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
    38941 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
    39001 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
    39061 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
    39121 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
    39181 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
    39241 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
    39301 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
    39361 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
    39421 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
    39481 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
    39541 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
    39601 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
    39661 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
    39721 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
    39781 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
    39841 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
    39901 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
    39961 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
    40021 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
    40081 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
    40141 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
    40201 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
    40261 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
    40321 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
    40381 ataaagctct gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt
    40441 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
    40501 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
    40561 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
    40621 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
    40681 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
    40741 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
    40801 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
    40861 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
    40921 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
    40981 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
    41041 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
    41101 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
    41161 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
    41221 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
    41281 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
    41341 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
    41401 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
    41461 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
    41521 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
    41581 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
    41641 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
    41701 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
    41761 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
    41821 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
    41881 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
    41941 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
    42001 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
    42061 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
    42121 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
    42181 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
    42241 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
    42301 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
    42361 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
    42421 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
    42481 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
    42541 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
    42601 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
    42661 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
    42721 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
    42781 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
    42841 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
    42901 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
    42961 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
    43021 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
    43081 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
    43141 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
    43201 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
    43261 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
    43321 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
    43381 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
    43441 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
    43501 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
    43561 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
    43621 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
    43681 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
    43741 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
    43801 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
    43861 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
    43921 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
    43981 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
    44041 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
    44101 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
    44161 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
    44221 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
    44281 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
    44341 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
    44401 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
    44461 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
    44521 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
    44581 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
    44641 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
    44701 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
    44761 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
    44821 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
    44881 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
    44941 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
    45001 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
    45061 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
    45121 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
    45181 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
    45241 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
    45301 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
    45361 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
    45421 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
    45481 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
    45541 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
    45601 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
    45661 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
    45721 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
    45781 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
    45841 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
    45901 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
    45961 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
    46021 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
    46081 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
    46141 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
    46201 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
    46261 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
    46321 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
    46381 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
    46441 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
    46501 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
    46561 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
    46621 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
    46681 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
    46741 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
    46801 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
    46861 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
    46921 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
    46981 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
    47041 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
    47101 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
    47161 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
    47221 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
    47281 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
    47341 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
    47401 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
    47461 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
    47521 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
    47581 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
    47641 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
    47701 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
    47761 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
    47821 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
    47881 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
    47941 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
    48001 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
    48061 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
    48121 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
    48181 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
    48241 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
    48301 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
    48361 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
    48421 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
    48481 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
    48541 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
    48601 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
    48661 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
    48721 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
    48781 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
    48841 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
    48901 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
    48961 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
    49021 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
    49081 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
    49141 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
    49201 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
    49261 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
    49321 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
    49381 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
    49441 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
    49501 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
    49561 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
    49621 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
    49681 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
    49741 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
    49801 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
    49861 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
    49921 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
    49981 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
    50041 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
    50101 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
    50161 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
    50221 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
    50281 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
    50341 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
    50401 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
    50461 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
    50521 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
    50581 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
    50641 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
    50701 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
    50761 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
    50821 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
    50881 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
    50941 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
    51001 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
    51061 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
    51121 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
    51181 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
    51241 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
    51301 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
    51361 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
    51421 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
    51481 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
    51541 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
    51601 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
    51661 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
    51721 aatcctcttt ttctcaccgg ttttcct
//
LOCUS       DAZKPO010000008        46542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000008 DAZKPO010000000
VERSION     DAZKPO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.008"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..909
                     /locus_tag="ACG8NZ_000478"
     CDS             28..909
                     /locus_tag="ACG8NZ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0538180.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="ACG8NZ_000479"
     CDS             922..2532
                     /locus_tag="ACG8NZ_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538181.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="ACG8NZ_000480"
     CDS             complement(2609..3268)
                     /locus_tag="ACG8NZ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0538182.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="ACG8NZ_000481"
     CDS             complement(3551..4216)
                     /locus_tag="ACG8NZ_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0538183.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8NZ_000482"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8NZ_000482"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0538184.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8NZ_000483"
     CDS             complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8NZ_000483"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0538185.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8NZ_000484"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8NZ_000484"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0538186.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="ACG8NZ_000485"
     CDS             6530..6850
                     /locus_tag="ACG8NZ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0538187.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7819)
                     /locus_tag="ACG8NZ_000486"
     CDS             complement(6962..7819)
                     /locus_tag="ACG8NZ_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0538188.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7927..9231)
                     /locus_tag="ACG8NZ_000487"
     CDS             complement(7927..9231)
                     /locus_tag="ACG8NZ_000487"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0538189.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9228..9737)
                     /locus_tag="ACG8NZ_000488"
     CDS             complement(9228..9737)
                     /locus_tag="ACG8NZ_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538190.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9912..12047)
                     /locus_tag="ACG8NZ_000489"
     CDS             complement(9912..12047)
                     /locus_tag="ACG8NZ_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0538191.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12130..13785)
                     /locus_tag="ACG8NZ_000490"
     CDS             complement(12130..13785)
                     /locus_tag="ACG8NZ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0538192.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13787..14101)
                     /locus_tag="ACG8NZ_000491"
     CDS             complement(13787..14101)
                     /locus_tag="ACG8NZ_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0538193.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14271..14531
                     /locus_tag="ACG8NZ_000492"
     CDS             14271..14531
                     /locus_tag="ACG8NZ_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0538194.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14545..14859
                     /locus_tag="ACG8NZ_000493"
     CDS             14545..14859
                     /locus_tag="ACG8NZ_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0538195.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15589..16143)
                     /locus_tag="ACG8NZ_000494"
     CDS             complement(15589..16143)
                     /locus_tag="ACG8NZ_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0538196.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16269..18198)
                     /locus_tag="ACG8NZ_000495"
                     /pseudo
     CDS             complement(16269..18198)
                     /locus_tag="ACG8NZ_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18504..19220)
                     /locus_tag="ACG8NZ_000496"
     CDS             complement(18504..19220)
                     /locus_tag="ACG8NZ_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0538197.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="ACG8NZ_000497"
     CDS             complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="ACG8NZ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0538198.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="ACG8NZ_000498"
     CDS             complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="ACG8NZ_000498"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0538199.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22690..24150)
                     /locus_tag="ACG8NZ_000499"
     CDS             complement(22690..24150)
                     /locus_tag="ACG8NZ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538200.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24140..25435)
                     /locus_tag="ACG8NZ_000500"
     CDS             complement(24140..25435)
                     /locus_tag="ACG8NZ_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0538201.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25767..26444)
                     /locus_tag="ACG8NZ_000501"
     CDS             complement(25767..26444)
                     /locus_tag="ACG8NZ_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0538202.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26474..27565)
                     /locus_tag="ACG8NZ_000502"
     CDS             complement(26474..27565)
                     /locus_tag="ACG8NZ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0538203.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27666..28100)
                     /locus_tag="ACG8NZ_000503"
     CDS             complement(27666..28100)
                     /locus_tag="ACG8NZ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0538204.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28684..30141)
                     /locus_tag="ACG8NZ_000504"
     CDS             complement(28684..30141)
                     /locus_tag="ACG8NZ_000504"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0538205.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30138..32033)
                     /locus_tag="ACG8NZ_000505"
     CDS             complement(30138..32033)
                     /locus_tag="ACG8NZ_000505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0538206.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32091..33866)
                     /locus_tag="ACG8NZ_000506"
     CDS             complement(32091..33866)
                     /locus_tag="ACG8NZ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0538207.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33916..35412)
                     /locus_tag="ACG8NZ_000507"
     CDS             complement(33916..35412)
                     /locus_tag="ACG8NZ_000507"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0538208.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35606..37228)
                     /locus_tag="ACG8NZ_000508"
     CDS             complement(35606..37228)
                     /locus_tag="ACG8NZ_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0538209.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37556..38869)
                     /locus_tag="ACG8NZ_000509"
     CDS             complement(37556..38869)
                     /locus_tag="ACG8NZ_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0538210.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39114..39338)
                     /locus_tag="ACG8NZ_000510"
     CDS             complement(39114..39338)
                     /locus_tag="ACG8NZ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538211.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39358..39780)
                     /locus_tag="ACG8NZ_000511"
     CDS             complement(39358..39780)
                     /locus_tag="ACG8NZ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0538212.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39922..40632)
                     /locus_tag="ACG8NZ_000512"
     CDS             complement(39922..40632)
                     /locus_tag="ACG8NZ_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0538213.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40650..41147)
                     /locus_tag="ACG8NZ_000513"
     CDS             complement(40650..41147)
                     /locus_tag="ACG8NZ_000513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0538214.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41184..41636)
                     /locus_tag="ACG8NZ_000514"
     CDS             complement(41184..41636)
                     /locus_tag="ACG8NZ_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0538215.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41771..42376)
                     /locus_tag="ACG8NZ_000515"
     CDS             complement(41771..42376)
                     /locus_tag="ACG8NZ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0538216.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42826..43446)
                     /locus_tag="ACG8NZ_000516"
     CDS             complement(42826..43446)
                     /locus_tag="ACG8NZ_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0538217.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43448..44317)
                     /locus_tag="ACG8NZ_000517"
     CDS             complement(43448..44317)
                     /locus_tag="ACG8NZ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0538218.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44340..44747)
                     /locus_tag="ACG8NZ_000518"
     CDS             complement(44340..44747)
                     /locus_tag="ACG8NZ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0538219.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44766..46055)
                     /locus_tag="ACG8NZ_000519"
     CDS             complement(44766..46055)
                     /locus_tag="ACG8NZ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0538220.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46113..>46542
                     /locus_tag="ACG8NZ_000520"
     CDS             46113..>46542
                     /locus_tag="ACG8NZ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0538221.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        13108 a         9901 c         7985 g        15548 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat
     9181 agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc
     9241 accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag
     9301 acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact
     9361 tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt
     9421 acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa
     9481 ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa
     9541 aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat
     9601 ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt
     9661 aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct
     9721 cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca
     9781 tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa
     9841 catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt
     9901 ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc
     9961 gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag
    10021 attacatgat caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct
    10081 aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg
    10141 gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac
    10201 aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat
    10261 acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg
    10321 ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa
    10381 caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct
    10441 actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta
    10501 taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca
    10561 agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa
    10621 gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc
    10681 catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt
    10741 gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac
    10801 tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc
    10861 aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc
    10921 aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact
    10981 gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct
    11041 gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca
    11101 ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc
    11161 tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg
    11221 acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat
    11281 gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt
    11341 ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt
    11401 gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg
    11461 cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc
    11521 aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt
    11581 tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt
    11641 gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata
    11701 taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga
    11761 cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc
    11821 atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag
    11881 taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct
    11941 atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac
    12001 acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc
    12061 tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt
    12121 taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga
    12181 aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag
    12241 atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt
    12301 taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc
    12361 caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa
    12421 ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag
    12481 catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg
    12541 taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc
    12601 cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat
    12661 caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac
    12721 atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga
    12781 tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg
    12841 agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag
    12901 agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc
    12961 ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc
    13021 taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat
    13081 aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc
    13141 aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc
    13201 tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac
    13261 catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc
    13321 accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc
    13381 tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac
    13441 aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc
    13501 ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg
    13561 acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc
    13621 ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata
    13681 cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc
    13741 agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta
    13801 ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt
    13861 tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg
    13921 tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt
    13981 ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca
    14041 aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca
    14101 ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt
    14161 tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa
    14221 aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg
    14281 ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa
    14341 aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt
    14401 ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga
    14461 aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta
    14521 ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa
    14581 tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt
    14641 gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc
    14701 ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt
    14761 tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt
    14821 gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa
    14881 gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg
    14941 aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    15001 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    15061 tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa
    15121 aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15181 cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    15241 aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    15301 taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac
    15361 ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15421 aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt
    15481 tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg
    15541 gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg
    15601 aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat
    15661 gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat
    15721 catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt
    15781 gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa
    15841 tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag
    15901 ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata
    15961 tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc
    16021 tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg
    16081 gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt
    16141 cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat
    16201 aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt
    16261 tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt
    16321 tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta
    16381 cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct
    16441 gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat
    16501 acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg
    16561 acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt
    16621 gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag
    16681 taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa
    16741 gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg
    16801 atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc
    16861 tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc
    16921 gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac
    16981 caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa
    17041 tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa
    17101 gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt
    17161 caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat
    17221 caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa
    17281 tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc
    17341 attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc
    17401 gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac
    17461 ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga
    17521 aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc
    17581 atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac
    17641 aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt
    17701 tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga
    17761 cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt ctacatttat
    17821 caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc
    17881 aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt
    17941 tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc
    18001 cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg
    18061 acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct
    18121 atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact
    18181 aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat
    18241 aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt
    18301 attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa
    18361 ctttgtttat tggctattcg acttactgat tagataccag ctacactcac aaaacatacc
    18421 ccattcttct aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc
    18481 aaactttagt atgttttata gatttacacc attaatgaat agacacctac cgcgataatt
    18541 gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta
    18601 ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt
    18661 gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg
    18721 aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat
    18781 acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct
    18841 gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa
    18901 gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga
    18961 acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc
    19021 aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa
    19081 ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc
    19141 gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt
    19201 tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca
    19261 atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat
    19321 ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac
    19381 agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa
    19441 ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt
    19501 gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct
    19561 tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat
    19621 ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc
    19681 tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc
    19741 ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct
    19801 aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg
    19861 ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta
    19921 tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca
    19981 gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc
    20041 aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct
    20101 tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag
    20161 taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg
    20221 aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta
    20281 gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc
    20341 caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc
    20401 aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc
    20461 attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa
    20521 cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc
    20581 aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt
    20641 cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg
    20701 aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg
    20761 tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca
    20821 tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc
    20881 ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg
    20941 actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc
    21001 tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta
    21061 ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg
    21121 ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc
    21181 tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt
    21241 ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag
    21301 atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc
    21361 atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt
    21421 aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg
    21481 atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct
    21541 tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc
    21601 tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac
    21661 ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt
    21721 ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg
    21781 ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg
    21841 tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt
    21901 gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc
    21961 tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc
    22021 ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc
    22081 tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc
    22141 tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc
    22201 attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata
    22261 gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc
    22321 aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga
    22381 ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata
    22441 ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc
    22501 tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat
    22561 tccctccatt atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa
    22621 aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct
    22681 attttttaat caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat
    22741 gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca
    22801 aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc
    22861 gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact
    22921 ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac
    22981 attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa
    23041 ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat
    23101 tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa
    23161 aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt
    23221 caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc
    23281 ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat
    23341 cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc
    23401 tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg
    23461 atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt
    23521 tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc
    23581 taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag
    23641 cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag
    23701 ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt
    23761 caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg
    23821 aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc
    23881 ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc
    23941 ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat
    24001 atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt
    24061 gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc
    24121 ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca
    24181 tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg
    24241 tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc
    24301 tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt
    24361 tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat
    24421 aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat
    24481 tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga
    24541 catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg
    24601 gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca
    24661 agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct
    24721 gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt
    24781 ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa
    24841 gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat
    24901 aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca
    24961 acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat
    25021 tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg
    25081 ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat
    25141 caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg
    25201 tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag
    25261 aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc
    25321 atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa
    25381 gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca
    25441 ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat
    25501 aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac
    25561 cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc
    25621 tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc
    25681 tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat
    25741 agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct
    25801 aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact
    25861 tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct
    25921 aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc
    25981 actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca
    26041 acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc
    26101 ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc
    26161 gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt
    26221 ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc
    26281 ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt
    26341 actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc
    26401 ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc
    26461 tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat
    26521 aaagctcttt atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca
    26581 ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg
    26641 catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag
    26701 ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat
    26761 cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct agttttgact
    26821 cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt
    26881 cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg
    26941 agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc
    27001 attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg
    27061 ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa
    27121 atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag
    27181 gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac
    27241 ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg
    27301 ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg
    27361 gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt
    27421 tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga
    27481 gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag
    27541 taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta
    27601 tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa
    27661 aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta
    27721 cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg
    27781 acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg
    27841 ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat
    27901 ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat
    27961 atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct
    28021 aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct
    28081 ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt
    28141 tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag
    28201 aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt
    28261 gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat
    28321 ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg
    28381 tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa
    28441 aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac
    28501 cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa
    28561 aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat
    28621 actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt
    28681 ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt
    28741 ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata
    28801 acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata
    28861 accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa
    28921 atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc
    28981 gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc
    29041 aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt
    29101 tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact
    29161 gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat
    29221 ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt
    29281 atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg
    29341 ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct
    29401 gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc
    29461 acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt
    29521 ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa
    29581 agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac
    29641 gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg
    29701 catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa
    29761 cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa
    29821 aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg
    29881 gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt
    29941 gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt
    30001 ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt
    30061 ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa
    30121 tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta
    30181 tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt
    30241 gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg
    30301 cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc
    30361 actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt
    30421 gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg
    30481 tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt
    30541 acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag
    30601 gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc
    30661 atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa
    30721 gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca
    30781 ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca
    30841 ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa
    30901 ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca
    30961 gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg
    31021 gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg
    31081 ccgataacag caccagataa aacagggcta aagttataaa cagctaaata tccagctgca
    31141 atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc
    31201 acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga
    31261 atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt
    31321 gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat
    31381 gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct
    31441 gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca
    31501 tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc
    31561 gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg
    31621 gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct
    31681 ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact
    31741 tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc
    31801 acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag
    31861 cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg
    31921 acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc
    31981 gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta
    32041 gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc
    32101 aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca
    32161 aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct
    32221 gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac
    32281 ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta
    32341 tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt
    32401 tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca
    32461 tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta
    32521 gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt
    32581 tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta
    32641 ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga
    32701 ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat
    32761 tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg
    32821 atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt
    32881 ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa
    32941 aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca
    33001 tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga
    33061 ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga
    33121 ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa
    33181 acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata
    33241 agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt
    33301 tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt
    33361 ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct
    33421 aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt
    33481 tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat
    33541 aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc
    33601 acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa
    33661 cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt tttgatttca
    33721 gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa
    33781 tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc
    33841 ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt
    33901 gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg
    33961 gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc
    34021 atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc
    34081 cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat
    34141 tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat
    34201 tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg
    34261 taattcataa cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg
    34321 ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc
    34381 gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa
    34441 tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg
    34501 tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat
    34561 gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg
    34621 atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc
    34681 atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg
    34741 acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc
    34801 agggaatgta accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt
    34861 aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa
    34921 ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa
    34981 gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt
    35041 gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg
    35101 aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa
    35161 acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag
    35221 tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg
    35281 ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca
    35341 tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat
    35401 ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg
    35461 attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct
    35521 actcttcatt tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca
    35581 ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc
    35641 gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta
    35701 aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc
    35761 attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta
    35821 attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt
    35881 acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat
    35941 ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc
    36001 attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct
    36061 cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg
    36121 cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat
    36181 aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat
    36241 acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata
    36301 aactattcca tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt
    36361 cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta
    36421 tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa taaagtttag
    36481 cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag
    36541 ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt gtgatttctt
    36601 tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat
    36661 cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca
    36721 aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact
    36781 gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat
    36841 cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc
    36901 acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt
    36961 tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg
    37021 cttgataatt actgatatca tacccattgt catcattcgg agactcgtat actggagaaa
    37081 gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca
    37141 aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa
    37201 ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt
    37261 tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta
    37321 ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac
    37381 tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa
    37441 acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact
    37501 attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt
    37561 tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag
    37621 atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg
    37681 caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga
    37741 tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta
    37801 ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga
    37861 acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa
    37921 cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac
    37981 ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt
    38041 ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag
    38101 tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat
    38161 gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag
    38221 gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga
    38281 tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca
    38341 accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga
    38401 tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg
    38461 catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga
    38521 tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc
    38581 ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc
    38641 caattggata acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag
    38701 tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa
    38761 ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg
    38821 tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc
    38881 ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt
    38941 gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat
    39001 ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat
    39061 tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag
    39121 tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca
    39181 ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc
    39241 ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga
    39301 tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca
    39361 atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa
    39421 agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt
    39481 ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa
    39541 cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac
    39601 aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc
    39661 tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg
    39721 tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat
    39781 tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag
    39841 tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc
    39901 gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg
    39961 tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg
    40021 tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt
    40081 ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc
    40141 ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa
    40201 aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc
    40261 gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac
    40321 aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat
    40381 agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat
    40441 gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc
    40501 attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt
    40561 tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat
    40621 tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct
    40681 gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta
    40741 cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt
    40801 tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt
    40861 ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca
    40921 gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact
    40981 aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct
    41041 tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat
    41101 gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg
    41161 tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc
    41221 caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct
    41281 tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa
    41341 ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta
    41401 tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt
    41461 gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa
    41521 atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg
    41581 ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca
    41641 tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt
    41701 ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt
    41761 acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct
    41821 tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg
    41881 aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa
    41941 gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc
    42001 gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt
    42061 cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt
    42121 tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg
    42181 gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc
    42241 ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa
    42301 atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag
    42361 ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag
    42421 tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga
    42481 atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg
    42541 ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc
    42601 ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac
    42661 ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg
    42721 tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta
    42781 aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt
    42841 gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac
    42901 atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt
    42961 tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc
    43021 atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta
    43081 ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc
    43141 gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga
    43201 cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata
    43261 aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc
    43321 atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag
    43381 tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt
    43441 tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct
    43501 tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact
    43561 tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct
    43621 ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt
    43681 tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca
    43741 gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa
    43801 tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat
    43861 ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt
    43921 ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga
    43981 tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga
    44041 ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca
    44101 tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag
    44161 cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca
    44221 tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt
    44281 ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt
    44341 tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc
    44401 atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta
    44461 tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt
    44521 tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt
    44581 tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt
    44641 gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc
    44701 agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact
    44761 ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct
    44821 actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact
    44881 agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct
    44941 cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt
    45001 agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc
    45061 gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa
    45121 actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat
    45181 gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact
    45241 acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca
    45301 gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc
    45361 atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg
    45421 atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag
    45481 tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg
    45541 atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc
    45601 aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc
    45661 atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa
    45721 ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa
    45781 tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat
    45841 ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca
    45901 ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat
    45961 ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc
    46021 aaaatatttt cgatgataaa attgataacg cccatgtttt gatgtgcacc ccaaaagtta
    46081 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
    46141 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
    46201 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
    46261 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
    46321 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
    46381 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
    46441 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
    46501 aagatgagtc tcttgaagaa gaagttattc gtttaagagc ag
//
LOCUS       DAZKPO010000009        45769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000009 DAZKPO010000000
VERSION     DAZKPO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.009"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG8NZ_000521"
     CDS             <1..730
                     /locus_tag="ACG8NZ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0538222.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="ACG8NZ_000522"
     CDS             788..2827
                     /locus_tag="ACG8NZ_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0538223.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="ACG8NZ_000523"
     CDS             complement(2936..4087)
                     /locus_tag="ACG8NZ_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0538224.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="ACG8NZ_000524"
     CDS             complement(4241..4771)
                     /locus_tag="ACG8NZ_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0538225.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="ACG8NZ_000525"
     CDS             complement(4870..5220)
                     /locus_tag="ACG8NZ_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0538226.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8NZ_000526"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8NZ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0538227.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8NZ_000527"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8NZ_000527"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0538228.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="ACG8NZ_000528"
     CDS             complement(7623..8285)
                     /locus_tag="ACG8NZ_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0538229.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="ACG8NZ_000529"
     CDS             8469..10115
                     /locus_tag="ACG8NZ_000529"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0538230.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8NZ_000530"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8NZ_000530"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0538231.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..12849)
                     /locus_tag="ACG8NZ_000531"
     CDS             complement(11296..12849)
                     /locus_tag="ACG8NZ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538232.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12997..14166
                     /locus_tag="ACG8NZ_000532"
     CDS             12997..14166
                     /locus_tag="ACG8NZ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0538233.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14292..15371)
                     /locus_tag="ACG8NZ_000533"
     CDS             complement(14292..15371)
                     /locus_tag="ACG8NZ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0538234.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15480..15920)
                     /locus_tag="ACG8NZ_000534"
     CDS             complement(15480..15920)
                     /locus_tag="ACG8NZ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0538235.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(15977..16387)
                     /locus_tag="ACG8NZ_000535"
     CDS             complement(15977..16387)
                     /locus_tag="ACG8NZ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0538236.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16568..17002)
                     /locus_tag="ACG8NZ_000536"
     CDS             complement(16568..17002)
                     /locus_tag="ACG8NZ_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0538237.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17102..17950)
                     /locus_tag="ACG8NZ_000537"
     CDS             complement(17102..17950)
                     /locus_tag="ACG8NZ_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0538238.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18339..20105
                     /locus_tag="ACG8NZ_000538"
     CDS             18339..20105
                     /locus_tag="ACG8NZ_000538"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0538239.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20207..21466
                     /locus_tag="ACG8NZ_000539"
     CDS             20207..21466
                     /locus_tag="ACG8NZ_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0538240.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21613..22914
                     /locus_tag="ACG8NZ_000540"
     CDS             21613..22914
                     /locus_tag="ACG8NZ_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0538241.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            22917..23768
                     /locus_tag="ACG8NZ_000541"
     CDS             22917..23768
                     /locus_tag="ACG8NZ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0538242.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23781..24596
                     /locus_tag="ACG8NZ_000542"
     CDS             23781..24596
                     /locus_tag="ACG8NZ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0538243.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24747..25913
                     /locus_tag="ACG8NZ_000543"
     CDS             24747..25913
                     /locus_tag="ACG8NZ_000543"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0538244.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26143..26790
                     /locus_tag="ACG8NZ_000544"
     CDS             26143..26790
                     /locus_tag="ACG8NZ_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0538245.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26874..27818
                     /locus_tag="ACG8NZ_000545"
     CDS             26874..27818
                     /locus_tag="ACG8NZ_000545"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0538246.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            27953..29404
                     /locus_tag="ACG8NZ_000546"
     CDS             27953..29404
                     /locus_tag="ACG8NZ_000546"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0538247.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29425..30261
                     /locus_tag="ACG8NZ_000547"
     CDS             29425..30261
                     /locus_tag="ACG8NZ_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0538248.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30261..30920
                     /locus_tag="ACG8NZ_000548"
     CDS             30261..30920
                     /locus_tag="ACG8NZ_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0538249.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            31046..31735
                     /locus_tag="ACG8NZ_000549"
     CDS             31046..31735
                     /locus_tag="ACG8NZ_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538250.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31741..32697
                     /locus_tag="ACG8NZ_000550"
     CDS             31741..32697
                     /locus_tag="ACG8NZ_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0538251.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32741..33253
                     /locus_tag="ACG8NZ_000551"
     CDS             32741..33253
                     /locus_tag="ACG8NZ_000551"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0538252.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33509..34057)
                     /locus_tag="ACG8NZ_000552"
     CDS             complement(33509..34057)
                     /locus_tag="ACG8NZ_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0538253.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34146..35327)
                     /locus_tag="ACG8NZ_000553"
     CDS             complement(34146..35327)
                     /locus_tag="ACG8NZ_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0538254.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35563..37071)
                     /locus_tag="ACG8NZ_000554"
     CDS             complement(35563..37071)
                     /locus_tag="ACG8NZ_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0538255.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37230..37421
                     /locus_tag="ACG8NZ_000555"
     CDS             37230..37421
                     /locus_tag="ACG8NZ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538256.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37624..38541
                     /locus_tag="ACG8NZ_000556"
     CDS             37624..38541
                     /locus_tag="ACG8NZ_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0538257.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38534..39607
                     /locus_tag="ACG8NZ_000557"
     CDS             38534..39607
                     /locus_tag="ACG8NZ_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538258.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39755..40273
                     /locus_tag="ACG8NZ_000558"
     CDS             39755..40273
                     /locus_tag="ACG8NZ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538259.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40254..40646
                     /locus_tag="ACG8NZ_000559"
     CDS             40254..40646
                     /locus_tag="ACG8NZ_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0538260.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40722..41657
                     /locus_tag="ACG8NZ_000560"
     CDS             40722..41657
                     /locus_tag="ACG8NZ_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0538261.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41790..42569
                     /locus_tag="ACG8NZ_000561"
     CDS             41790..42569
                     /locus_tag="ACG8NZ_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0538262.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42562..42879
                     /locus_tag="ACG8NZ_000562"
     CDS             42562..42879
                     /locus_tag="ACG8NZ_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0538263.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43123..44733
                     /locus_tag="ACG8NZ_000563"
     CDS             43123..44733
                     /locus_tag="ACG8NZ_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0538264.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            44890..45738
                     /locus_tag="ACG8NZ_000564"
     CDS             44890..45738
                     /locus_tag="ACG8NZ_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0538265.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15181 a         8248 c         8803 g        13537 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12721 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    12781 aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg
    12841 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
    12901 tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat
    12961 ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata
    13021 tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag
    13081 atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag
    13141 gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc
    13201 atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc agctgttaca
    13261 tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt gactatccaa
    13321 cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg
    13381 ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt aacgccgtat
    13441 agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga tcttgttgaa
    13501 gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac agaaaatcca
    13561 gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga aactcaacca
    13621 gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga tgtagaccca
    13681 gaagaaaaac cggatgtgac tccagaacca gatacggatt ctggaaacca aacagtaccg
    13741 gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaactaga ggtagatcca
    13801 gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca aggaatacca
    13861 gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa aaaatcaaat
    13921 ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat atttgatagc
    13981 gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctag tcctgaaacg
    14041 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    14101 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    14161 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    14221 acgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    14281 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    14341 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    14401 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    14461 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    14521 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    14581 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    14641 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtgttt
    14701 aaaagagcga taacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    14761 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    14821 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    14881 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    14941 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    15001 tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat atcaaatgtt
    15061 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    15121 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    15181 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    15241 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    15301 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    15361 acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa aaagaaaaac
    15421 cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt
    15481 cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt
    15541 gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct
    15601 gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat tctttcatca
    15661 cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc
    15721 agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt
    15781 tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg
    15841 gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca tacttgatta
    15901 actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg
    15961 atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg
    16021 tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca
    16081 gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt
    16141 ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt
    16201 tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag
    16261 ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat
    16321 cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt
    16381 ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac acaatataat
    16441 tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac
    16501 gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac taatctcatc
    16561 ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt acttcacgat
    16621 ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac
    16681 caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat
    16741 tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag
    16801 cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtgccat
    16861 acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta
    16921 tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat
    16981 ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga
    17041 ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa
    17101 ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa
    17161 tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat
    17221 cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta
    17281 gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat
    17341 gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga
    17401 caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaacaa
    17461 acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt
    17521 ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt
    17581 tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc
    17641 gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa aacaagcttc
    17701 cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa
    17761 cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct tccagtaata
    17821 ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag
    17881 cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg
    17941 tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta ttataactta
    18001 cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa attattatgt
    18061 ttcattcttc tagaatatac ttctacgtta aaaactgtta aaaaactttt actttagaaa
    18121 attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag gaagtagatt
    18181 aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta
    18241 tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgtttct ctatcactta
    18301 tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc
    18361 atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc
    18421 gtactaggaa aaatgatatc aaaaaagtat atgtgctttg tggagatcca tataccataa
    18481 caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc
    18541 atgattattg ggaaattgaa gtgacgagcg aaacaaggcg cttacagtat gcgttccatg
    18601 ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag
    18661 aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc
    18721 gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat
    18781 ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc
    18841 cagaccgaga agacttcttc gggggagatt tgcaaggtgt gagggatcat ttggattact
    18901 tgacggacct tggcgtcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc
    18961 ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt
    19021 taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat
    19081 tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat
    19141 cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg
    19201 acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc
    19261 cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt
    19321 ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc
    19381 atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg
    19441 gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga
    19501 attatgcatt cacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac
    19561 aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa
    19621 cattcaactt attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca
    19681 aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct
    19741 attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga
    19801 tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc
    19861 taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta atagtatatg
    19921 atcgtgaaga taagctctat ttcacacggg aactagaggg acagatcatc tatgtttact
    19981 ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa
    20041 attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt
    20101 gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt
    20161 ataataaggg tgtaacagca cgttgaatac acaggaggaa atgaagatga aaaaaagaag
    20221 tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg
    20281 tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc
    20341 cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg aaaaagaaaa
    20401 cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt
    20461 agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc
    20521 gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga
    20581 aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga
    20641 gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga
    20701 tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac
    20761 aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg
    20821 tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg
    20881 tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat
    20941 gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc
    21001 agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg
    21061 tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa
    21121 aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta
    21181 tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa
    21241 ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga
    21301 acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg
    21361 tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga
    21421 tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc
    21481 gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga
    21541 aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga
    21601 gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt
    21661 tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt
    21721 tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg
    21781 gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt tattttgatt
    21841 gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt
    21901 ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg
    21961 aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct
    22021 tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt
    22081 actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa
    22141 aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg
    22201 tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca
    22261 gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta
    22321 ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat
    22381 agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat
    22441 atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg
    22501 cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt
    22561 tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca
    22621 aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg
    22681 gggaatttca ataatttctc catgatctat ctgtttaaca atggtggtcc cggaagcgta
    22741 ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact tacgactgga
    22801 acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc
    22861 tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga
    22921 aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt
    22981 ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct
    23041 tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa
    23101 actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca
    23161 tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata
    23221 gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg
    23281 taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg
    23341 ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc
    23401 tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg
    23461 gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg
    23521 ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc
    23581 tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg
    23641 tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt
    23701 gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa
    23761 aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta
    23821 cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat
    23881 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
    23941 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
    24001 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
    24061 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
    24121 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
    24181 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
    24241 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
    24301 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
    24361 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
    24421 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
    24481 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
    24541 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
    24601 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
    24661 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
    24721 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
    24781 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
    24841 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
    24901 agattacaag taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga
    24961 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
    25021 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
    25081 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
    25141 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
    25201 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
    25261 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
    25321 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
    25381 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
    25441 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
    25501 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
    25561 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
    25621 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
    25681 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
    25741 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
    25801 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
    25861 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
    25921 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
    25981 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
    26041 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
    26101 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
    26161 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
    26221 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
    26281 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
    26341 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
    26401 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
    26461 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
    26521 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
    26581 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
    26641 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
    26701 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
    26761 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
    26821 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
    26881 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
    26941 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
    27001 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
    27061 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
    27121 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
    27181 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
    27241 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
    27301 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
    27361 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
    27421 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
    27481 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
    27541 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
    27601 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
    27661 tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa gggccggaaa
    27721 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
    27781 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
    27841 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
    27901 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
    27961 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
    28021 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
    28081 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
    28141 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
    28201 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
    28261 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
    28321 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
    28381 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
    28441 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
    28501 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
    28561 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
    28621 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
    28681 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
    28741 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
    28801 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
    28861 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
    28921 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
    28981 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
    29041 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
    29101 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
    29161 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
    29221 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
    29281 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
    29341 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
    29401 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
    29461 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
    29521 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
    29581 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
    29641 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
    29701 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
    29761 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
    29821 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
    29881 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
    29941 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
    30001 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
    30061 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
    30121 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
    30181 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
    30241 gctgagtcgg tgagtgagtg atgtactttc ccctaattga tttttccctc atgtggactt
    30301 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
    30361 ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
    30421 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
    30481 tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
    30541 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
    30601 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
    30661 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
    30721 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
    30781 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
    30841 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
    30901 ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
    30961 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
    31021 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
    31081 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
    31141 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
    31201 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
    31261 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
    31321 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
    31381 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
    31441 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
    31501 aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat tgaaaagacg
    31561 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
    31621 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
    31681 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
    31741 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
    31801 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
    31861 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
    31921 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
    31981 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
    32041 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
    32101 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
    32161 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
    32221 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
    32281 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
    32341 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
    32401 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
    32461 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
    32521 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
    32581 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
    32641 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
    32701 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
    32761 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
    32821 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
    32881 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
    32941 attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
    33001 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
    33061 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
    33121 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
    33181 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
    33241 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
    33301 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
    33361 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
    33421 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gatctctcgg
    33481 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
    33541 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
    33601 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
    33661 aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag
    33721 gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg
    33781 cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat
    33841 gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat
    33901 tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg
    33961 tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag
    34021 aaccagaagg accgataaat acatagcaat aattcattgt catttctcct ttgatgattg
    34081 aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt
    34141 gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt
    34201 agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc
    34261 atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat
    34321 ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca
    34381 acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc
    34441 catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa
    34501 gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc aacaaaaccg
    34561 ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta ttctcccaac
    34621 atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc tttttcttct
    34681 aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt caaaacaagg
    34741 gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat cgacatagaa
    34801 actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag gaagggtaat
    34861 tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc tactccatta
    34921 taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac agctttcata
    34981 aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat aaatacaatt
    35041 ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca
    35101 aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc caatacagaa
    35161 acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg
    35221 aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat caaaaaagca
    35281 agccctaatg tgacaaataa accaataata cctaataaat aactcaaata atcactccta
    35341 ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt tagtgaaatt
    35401 ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa gcgaacaaaa
    35461 taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt
    35521 aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt aatatagatt
    35581 ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca atgtttgttc
    35641 atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt cagctagttt
    35701 atcataagca acttgagcga ttggattcgt tactgcactg attgataaag cgatcaagac
    35761 ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca atccttgtat
    35821 tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg ctaaatgttt
    35881 tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac gcccggtaat
    35941 gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg taggactttg
    36001 cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt cttcaggacg
    36061 taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt cgatcaatcc
    36121 ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt tagaggcttc
    36181 ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca tgtcagtagg
    36241 agattgataa acagattctt taccagtaat tttttcaata atacgtttga ttacaggaaa
    36301 tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa agtggaagga
    36361 atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa tgttcagcgg
    36421 atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac cagctgcatg
    36481 tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc cacttcctgg
    36541 gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat tttttccaaa
    36601 accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt ctgagtgttt
    36661 atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg caggctgtcc
    36721 actatagcga gtgataacga cactgttgat taataagcca taaccacgta attcatcgat
    36781 taaccgcaag atatccatat cataagtaat cccataatca ccacggatct tattacgttc
    36841 aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt tttgcagcaa
    36901 tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac
    36961 taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt ccaaaatata
    37021 cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca ctaggttccc
    37081 tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa
    37141 ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt attcattggt
    37201 atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat
    37261 ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa gatcccgaat
    37321 tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt gatgagcatc
    37381 agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga taaaggtcat
    37441 cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa ggctgtacca
    37501 aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac aattttttac
    37561 agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga ggaggcaagt
    37621 agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga ggagacgaac
    37681 gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga aaaagcaagc
    37741 atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt ccgcgaagaa
    37801 gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc
    37861 tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta
    37921 caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa
    37981 atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt
    38041 tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac
    38101 tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa
    38161 cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa
    38221 ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa
    38281 gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag
    38341 cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta
    38401 gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca
    38461 atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa
    38521 ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac
    38581 agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga
    38641 cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa aagtaactcc
    38701 gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta
    38761 tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc
    38821 agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt
    38881 agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag
    38941 cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg
    39001 gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa
    39061 tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt
    39121 ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga
    39181 ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat
    39241 cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac
    39301 cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt
    39361 tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta
    39421 cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt
    39481 aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc
    39541 acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa
    39601 gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact
    39661 tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga
    39721 ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct
    39781 tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt
    39841 ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt
    39901 aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt
    39961 catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag
    40021 aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt
    40081 ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat
    40141 ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt
    40201 tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa
    40261 aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta
    40321 caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa
    40381 caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc ttcaaaaaag
    40441 gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca
    40501 tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg
    40561 tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg
    40621 tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc
    40681 cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa
    40741 gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat
    40801 ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg
    40861 ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga
    40921 gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa
    40981 tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc
    41041 atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg
    41101 taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa
    41161 aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta
    41221 cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt
    41281 atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga
    41341 tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga
    41401 cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga
    41461 tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata
    41521 tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc
    41581 gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa
    41641 attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat
    41701 cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt
    41761 tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc
    41821 aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg
    41881 gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg
    41941 tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg
    42001 caacgacatt attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc
    42061 ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga
    42121 atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa
    42181 gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg
    42241 gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg
    42301 ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt
    42361 tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga
    42421 ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag
    42481 aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc
    42541 cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg
    42601 ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg
    42661 ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct
    42721 tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct
    42781 gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg
    42841 acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa
    42901 cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa
    42961 ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc
    43021 tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag
    43081 ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg
    43141 caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat
    43201 ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg
    43261 tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca
    43321 aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg
    43381 ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg
    43441 atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg
    43501 atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg
    43561 agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa
    43621 caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac
    43681 ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct
    43741 gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt
    43801 ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg
    43861 gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa
    43921 gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa
    43981 ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg
    44041 gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg
    44101 atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct
    44161 tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat
    44221 cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc
    44281 acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact
    44341 gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga
    44401 tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac
    44461 tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa
    44521 actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat
    44581 cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt
    44641 attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa
    44701 aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact
    44761 tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc
    44821 atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa
    44881 attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc
    44941 aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat
    45001 aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat
    45061 tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa
    45121 gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc
    45181 aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct
    45241 aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa
    45301 ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctatgct
    45361 tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca
    45421 gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt
    45481 gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt
    45541 ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat
    45601 ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc
    45661 gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt
    45721 gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DAZKPO010000010        45670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000010 DAZKPO010000000
VERSION     DAZKPO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.010"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8NZ_000565"
     CDS             6..245
                     /locus_tag="ACG8NZ_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538266.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8NZ_000566"
     CDS             312..521
                     /locus_tag="ACG8NZ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538267.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8NZ_000567"
     CDS             581..1252
                     /locus_tag="ACG8NZ_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0538268.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8NZ_000568"
     CDS             1305..3281
                     /locus_tag="ACG8NZ_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0538269.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG8NZ_000569"
     CDS             3294..4049
                     /locus_tag="ACG8NZ_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0538270.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG8NZ_000570"
     CDS             4046..4870
                     /locus_tag="ACG8NZ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0538271.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG8NZ_000571"
     CDS             4880..5128
                     /locus_tag="ACG8NZ_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538272.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG8NZ_000572"
     CDS             5171..7270
                     /locus_tag="ACG8NZ_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0538273.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG8NZ_000573"
     CDS             complement(7308..9365)
                     /locus_tag="ACG8NZ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0538274.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG8NZ_000574"
     CDS             complement(9479..9691)
                     /locus_tag="ACG8NZ_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538275.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG8NZ_000575"
     CDS             9878..10345
                     /locus_tag="ACG8NZ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538276.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG8NZ_000576"
     CDS             10342..10584
                     /locus_tag="ACG8NZ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0538277.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG8NZ_000577"
     CDS             10602..10904
                     /locus_tag="ACG8NZ_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538278.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG8NZ_000578"
     CDS             10976..13030
                     /locus_tag="ACG8NZ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538279.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG8NZ_000579"
     CDS             13030..15642
                     /locus_tag="ACG8NZ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0538280.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG8NZ_000580"
     CDS             15639..18170
                     /locus_tag="ACG8NZ_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0538281.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG8NZ_000581"
     CDS             18222..18554
                     /locus_tag="ACG8NZ_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538282.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG8NZ_000582"
     CDS             18555..19148
                     /locus_tag="ACG8NZ_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538283.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG8NZ_000583"
     CDS             19311..21005
                     /locus_tag="ACG8NZ_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0538284.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_000584"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_000584"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0538285.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG8NZ_000585"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG8NZ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG8NZ_000586"
     CDS             24078..25283
                     /locus_tag="ACG8NZ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0538286.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG8NZ_000587"
     CDS             25299..25943
                     /locus_tag="ACG8NZ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538287.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG8NZ_000588"
     CDS             25954..26760
                     /locus_tag="ACG8NZ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538288.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="ACG8NZ_000589"
     CDS             26782..27012
                     /locus_tag="ACG8NZ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538289.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="ACG8NZ_000590"
     CDS             27558..27833
                     /locus_tag="ACG8NZ_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538290.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="ACG8NZ_000591"
     CDS             27875..28297
                     /locus_tag="ACG8NZ_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538291.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8NZ_000592"
     CDS             28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8NZ_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0538292.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="ACG8NZ_000593"
     CDS             29920..30255
                     /locus_tag="ACG8NZ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538293.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="ACG8NZ_000594"
     CDS             30402..30596
                     /locus_tag="ACG8NZ_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538294.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="ACG8NZ_000595"
     CDS             30628..33000
                     /locus_tag="ACG8NZ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0538295.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="ACG8NZ_000596"
     CDS             33061..34521
                     /locus_tag="ACG8NZ_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0538296.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="ACG8NZ_000597"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="ACG8NZ_000597"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0538297.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36752..36901
                     /locus_tag="ACG8NZ_000598"
     CDS             36752..36901
                     /locus_tag="ACG8NZ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538298.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36975..37568
                     /locus_tag="ACG8NZ_000599"
     CDS             36975..37568
                     /locus_tag="ACG8NZ_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0538299.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="ACG8NZ_000600"
     CDS             37584..38483
                     /locus_tag="ACG8NZ_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0538300.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8NZ_000601"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8NZ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0538301.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="ACG8NZ_000602"
     CDS             39337..39597
                     /locus_tag="ACG8NZ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538302.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="ACG8NZ_000603"
     CDS             40038..40244
                     /locus_tag="ACG8NZ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0538303.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="ACG8NZ_000604"
     CDS             40244..40495
                     /locus_tag="ACG8NZ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538304.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="ACG8NZ_000605"
     CDS             40510..40947
                     /locus_tag="ACG8NZ_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538305.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="ACG8NZ_000606"
     CDS             40940..41647
                     /locus_tag="ACG8NZ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538306.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41812..42012
                     /locus_tag="ACG8NZ_000607"
     CDS             41812..42012
                     /locus_tag="ACG8NZ_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538307.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42028..42207
                     /locus_tag="ACG8NZ_000608"
     CDS             42028..42207
                     /locus_tag="ACG8NZ_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538308.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42548..42811
                     /locus_tag="ACG8NZ_000609"
     CDS             42548..42811
                     /locus_tag="ACG8NZ_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0538309.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42805..43155
                     /locus_tag="ACG8NZ_000610"
     CDS             42805..43155
                     /locus_tag="ACG8NZ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0538310.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43179..43793)
                     /locus_tag="ACG8NZ_000611"
     CDS             complement(43179..43793)
                     /locus_tag="ACG8NZ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0538311.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44107..44529
                     /locus_tag="ACG8NZ_000612"
     CDS             44107..44529
                     /locus_tag="ACG8NZ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538312.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44539..44742
                     /locus_tag="ACG8NZ_000613"
     CDS             44539..44742
                     /locus_tag="ACG8NZ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538313.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44953..45537
                     /locus_tag="ACG8NZ_000614"
     CDS             44953..45537
                     /locus_tag="ACG8NZ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0538314.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16743 a         7205 c         9261 g        12461 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa
    26461 gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 accttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacttcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
    34621 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
    34741 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
    34801 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
    34861 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
    35161 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
    35281 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
    35341 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
    35401 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
    35461 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
    35521 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
    35581 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
    35821 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
    35881 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
    42361 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
    42421 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    42481 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    42541 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    42601 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    42661 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    42721 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    42781 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    42841 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaagacctta tattgtttta
    42901 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    42961 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    43021 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    43081 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    43141 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccatgctg
    43201 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    43261 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    43321 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgcttttgca
    43381 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    43441 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    43501 tcggccatga tcggtagact tgtgatgatc agctgcacat ttttcttctt caagtaattg
    43561 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    43621 atatcttgat ctcggacgaa atccagtgct tcttgaaaaa caagtcgatg agtaatcgtt
    43681 gcgccagatt gtttctcaat gaaaattctc tctgcaccga attttttcat ttcttcaatc
    43741 tgccgttgtt cattttgatc gatggatgag acacgtatgt atgcaactct caaaattttt
    43801 acctactttc tgcaactatt taaaaaacac cctaatcata cacgagaaaa ccagattttt
    43861 caatgtcgcg gtcgtgtcta gttagggtat accttatata gacaaaaact aatgatggag
    43921 gaaatagtat gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat
    43981 caagaaaatt taaacgatta tgacattaaa aatatatttt atgaattttc tagtatataa
    44041 cttgatttat ctgttaagat ttattaagaa aacgttttct tatttttgat ttcgaggtga
    44101 tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta
    44161 actggaacta caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat
    44221 aataaccaag aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac
    44281 gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct
    44341 aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct
    44401 cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat
    44461 ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact
    44521 agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc
    44581 gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact
    44641 ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa
    44701 agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt
    44761 gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac
    44821 actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata
    44881 acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa
    44941 ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat
    45001 ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt
    45061 ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat
    45121 acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc
    45181 gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg
    45241 gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt
    45301 gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga
    45361 cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta
    45421 gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc
    45481 atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt
    45541 ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa
    45601 ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg
    45661 ttaatattaa
//
LOCUS       DAZKPO010000011        45486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000011 DAZKPO010000000
VERSION     DAZKPO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.011"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1729)
                     /locus_tag="ACG8NZ_000615"
                     /pseudo
     CDS             complement(94..1729)
                     /locus_tag="ACG8NZ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2052..2531)
                     /gene="rlmH"
                     /locus_tag="ACG8NZ_000616"
     CDS             complement(2052..2531)
                     /gene="rlmH"
                     /locus_tag="ACG8NZ_000616"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0538315.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2959..4248
                     /locus_tag="ACG8NZ_000617"
     CDS             2959..4248
                     /locus_tag="ACG8NZ_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0538316.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4287..4892)
                     /gene="ytpR"
                     /locus_tag="ACG8NZ_000618"
     CDS             complement(4287..4892)
                     /gene="ytpR"
                     /locus_tag="ACG8NZ_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0538317.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5097..5561)
                     /locus_tag="ACG8NZ_000619"
     CDS             complement(5097..5561)
                     /locus_tag="ACG8NZ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0538318.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5583..5903)
                     /locus_tag="ACG8NZ_000620"
     CDS             complement(5583..5903)
                     /locus_tag="ACG8NZ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0538319.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6047..7126)
                     /gene="pepA"
                     /locus_tag="ACG8NZ_000621"
     CDS             complement(6047..7126)
                     /gene="pepA"
                     /locus_tag="ACG8NZ_000621"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0538320.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7326..7676
                     /locus_tag="ACG8NZ_000622"
     CDS             7326..7676
                     /locus_tag="ACG8NZ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0538321.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7899..8846
                     /locus_tag="ACG8NZ_000623"
     CDS             7899..8846
                     /locus_tag="ACG8NZ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0538322.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8903..10321)
                     /gene="pepV"
                     /locus_tag="ACG8NZ_000624"
     CDS             complement(8903..10321)
                     /gene="pepV"
                     /locus_tag="ACG8NZ_000624"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0538323.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10470..11315
                     /locus_tag="ACG8NZ_000625"
     CDS             10470..11315
                     /locus_tag="ACG8NZ_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0538324.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11319..11759
                     /locus_tag="ACG8NZ_000626"
     CDS             11319..11759
                     /locus_tag="ACG8NZ_000626"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0538325.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11809..12003
                     /locus_tag="ACG8NZ_000627"
     CDS             11809..12003
                     /locus_tag="ACG8NZ_000627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538326.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12139..12825
                     /locus_tag="ACG8NZ_000628"
     CDS             12139..12825
                     /locus_tag="ACG8NZ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0538327.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13233..13922)
                     /locus_tag="ACG8NZ_000629"
     CDS             complement(13233..13922)
                     /locus_tag="ACG8NZ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538328.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13897..16026)
                     /locus_tag="ACG8NZ_000630"
     CDS             complement(13897..16026)
                     /locus_tag="ACG8NZ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0538329.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16074..17411)
                     /locus_tag="ACG8NZ_000631"
     CDS             complement(16074..17411)
                     /locus_tag="ACG8NZ_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0538330.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17663..18022)
                     /gene="rplT"
                     /locus_tag="ACG8NZ_000632"
     CDS             complement(17663..18022)
                     /gene="rplT"
                     /locus_tag="ACG8NZ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0538331.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18075..18275)
                     /gene="rpmI"
                     /locus_tag="ACG8NZ_000633"
     CDS             complement(18075..18275)
                     /gene="rpmI"
                     /locus_tag="ACG8NZ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0538332.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18347..18871)
                     /gene="infC"
                     /locus_tag="ACG8NZ_000634"
     CDS             complement(18347..18871)
                     /gene="infC"
                     /locus_tag="ACG8NZ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0538333.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18915..19040)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19191..19823
                     /locus_tag="ACG8NZ_000635"
     CDS             19191..19823
                     /locus_tag="ACG8NZ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0538334.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19825..20730)
                     /locus_tag="ACG8NZ_000636"
     CDS             complement(19825..20730)
                     /locus_tag="ACG8NZ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0538335.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20815..21474)
                     /locus_tag="ACG8NZ_000637"
     CDS             complement(20815..21474)
                     /locus_tag="ACG8NZ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0538336.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21790..22734
                     /gene="mvk"
                     /locus_tag="ACG8NZ_000638"
     CDS             21790..22734
                     /gene="mvk"
                     /locus_tag="ACG8NZ_000638"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0538337.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22727..23704
                     /gene="mvaD"
                     /locus_tag="ACG8NZ_000639"
     CDS             22727..23704
                     /gene="mvaD"
                     /locus_tag="ACG8NZ_000639"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0538338.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23717..24802
                     /locus_tag="ACG8NZ_000640"
     CDS             23717..24802
                     /locus_tag="ACG8NZ_000640"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0538339.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24799..25863
                     /gene="fni"
                     /locus_tag="ACG8NZ_000641"
     CDS             24799..25863
                     /gene="fni"
                     /locus_tag="ACG8NZ_000641"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0538340.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26112..26915)
                     /locus_tag="ACG8NZ_000642"
     CDS             complement(26112..26915)
                     /locus_tag="ACG8NZ_000642"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0538341.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26935..27777)
                     /gene="kduI"
                     /locus_tag="ACG8NZ_000643"
     CDS             complement(26935..27777)
                     /gene="kduI"
                     /locus_tag="ACG8NZ_000643"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0538342.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27793..28800)
                     /locus_tag="ACG8NZ_000644"
     CDS             complement(27793..28800)
                     /locus_tag="ACG8NZ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0538343.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28807..29451)
                     /locus_tag="ACG8NZ_000645"
     CDS             complement(28807..29451)
                     /locus_tag="ACG8NZ_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0538344.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29551..30309)
                     /locus_tag="ACG8NZ_000646"
     CDS             complement(29551..30309)
                     /locus_tag="ACG8NZ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0538345.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30436..31587)
                     /gene="fucO"
                     /locus_tag="ACG8NZ_000647"
     CDS             complement(30436..31587)
                     /gene="fucO"
                     /locus_tag="ACG8NZ_000647"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0538346.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31669..31980)
                     /gene="rhaM"
                     /locus_tag="ACG8NZ_000648"
     CDS             complement(31669..31980)
                     /gene="rhaM"
                     /locus_tag="ACG8NZ_000648"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0538347.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31982..32827)
                     /gene="rhaD"
                     /locus_tag="ACG8NZ_000649"
     CDS             complement(31982..32827)
                     /gene="rhaD"
                     /locus_tag="ACG8NZ_000649"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0538348.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32841..34097)
                     /gene="rhaA"
                     /locus_tag="ACG8NZ_000650"
     CDS             complement(32841..34097)
                     /gene="rhaA"
                     /locus_tag="ACG8NZ_000650"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0538349.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34094..35554)
                     /gene="rhaB"
                     /locus_tag="ACG8NZ_000651"
     CDS             complement(34094..35554)
                     /gene="rhaB"
                     /locus_tag="ACG8NZ_000651"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0538350.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35707..36576
                     /locus_tag="ACG8NZ_000652"
     CDS             35707..36576
                     /locus_tag="ACG8NZ_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0538351.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36702..37601)
                     /locus_tag="ACG8NZ_000653"
     CDS             complement(36702..37601)
                     /locus_tag="ACG8NZ_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538352.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37766..38590)
                     /locus_tag="ACG8NZ_000654"
     CDS             complement(37766..38590)
                     /locus_tag="ACG8NZ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0538353.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38601..39896)
                     /locus_tag="ACG8NZ_000655"
     CDS             complement(38601..39896)
                     /locus_tag="ACG8NZ_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0538354.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39912..40391)
                     /locus_tag="ACG8NZ_000656"
     CDS             complement(39912..40391)
                     /locus_tag="ACG8NZ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0538355.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40397..41425)
                     /locus_tag="ACG8NZ_000657"
     CDS             complement(40397..41425)
                     /locus_tag="ACG8NZ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0538356.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41442..42548)
                     /locus_tag="ACG8NZ_000658"
     CDS             complement(41442..42548)
                     /locus_tag="ACG8NZ_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0538357.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42777..45374)
                     /gene="adhE"
                     /locus_tag="ACG8NZ_000659"
                     /gene_synonym="adhC"
     CDS             complement(42777..45374)
                     /gene="adhE"
                     /locus_tag="ACG8NZ_000659"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0538358.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13164 a         9671 c         8252 g        14399 t
ORIGIN      
        1 gattaagctt cattattata atataaagtt taaatttggt ttattactta ctttttctta
       61 ttctctagta agctaatatt gaaaaaaatg taatcagatc attattttta tatgttgtaa
      121 cttcctgata agaaaactaa ttaaaagaaa gaggtaacta cataaactaa ttaaaaaagc
      181 taacttcata gttggaggat aaatggaaga aacttcaatg attgtttctc cttgtgttcc
      241 ttcaatcgac actagtccta ttatcaatct ctcttttttg tccatttact tttgcgactg
      301 ttccccaata atcaaataca ggaagcgtta ttttttgtcc tttagattca tgtactgtat
      361 agactatttt cgaatccatc acttgataag gcatagtatg ccattccttg ccaattctta
      421 cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc cgtgggtgca tagtcataaa
      481 gtcctccggg aaggagtgtc gtgtaacgaa taagattttc ttgattcatt accaagtgat
      541 ctctctgagt gacaacttta tgttcatagt ttgcttttgt tgcagcagat acagccaaca
      601 gtaataaaac aattgccgtt atattaacta cttttctttg cttagctgat gtgctcttca
      661 tgaaaacgaa cgataaaatc aacgagccaa acagtacttg aaagcctaag attcgccaag
      721 gaaattgaat catttgaatg ggtgtatttt gtaataaaga ccatggaaaa aatttggtgg
      781 ataagagcca agagactacc gcactaaggt aaatcacttt ttctttgcga ttaaatcctt
      841 taaaaaagaa gagtggacag atcaaagaaa taataccaat gattcctacc gtaaaggttg
      901 tcaggtcatt attcatcgca ccaatcaaac tatccgagac atttaatgct tggtgttgta
      961 aggtcgtttt atctggctca ttaatcgatt gaaccatgat ttgtcggaac atcggatacc
     1021 aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt tgacaactcc acaatcaacc
     1081 ggatgctttc tttttttctt gtttcactta ctacaaagcc gcctaagact agtaagccaa
     1141 cggctattac actcataaaa gtggaaagaa catgtgtata aatgagtaag ctcatactga
     1201 ctgctaatgg aattgcacat ggctttttct cataaatcag tgcgtaagca taatacataa
     1261 ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc agaacgatga taaatatcaa
     1321 tcgtacggta cccataaaat gtatataaac agctaaatat aaaagccgca tcgattcttt
     1381 ttaaaaattt tagtccataa tgaaacatca gcatgatagt acaaacattc aatagccaaa
     1441 catataagat ataactaacg attaaattat tacttatcca ataaaaaatg actgccggaa
     1501 aaaaggtcaa gtagggataa aaataattca ctccgctccc ataatgatta aacgtcgtaa
     1561 aattgattgg accagaaaaa attgaagata accctttgac tctagcaaaa tggaaggcaa
     1621 tatcccccga tgtagaaata gtatgaacat ttctaaaata attgattgaa taaagtccag
     1681 caataattgc taggagaatg gacaatatta tttttttctt tacttgcatt acttaattat
     1741 atactccttt catggattgc tttatctagt ataacttaaa ttttaaataa atcgttattt
     1801 atttttactc tcacttaaaa aagcactaaa attataagag agggaggtaa tggtctgaac
     1861 accaagaatt gttccaacat cgaattgctt aactaatgag ttcaaagctt aattatgtgg
     1921 ctcttatatt taccgagcgt ggattgcgat ctctttagcg aaacaaacat ctttcataaa
     1981 aaaaacagcc atatccgctt tcttggatat gactatttac tactacttta ctatctttca
     2041 ttcttcttgt ctcacttatg ataaggcgcc cccgcattga tccggaaggc acggtagatt
     2101 tgctcgacaa ggatcagacg catcaattga tgtggatacg tcatttttcc aaaagagatc
     2161 tgagcattgc ttcttttcat gacggcttcg ctgagtccta aagagccgcc gataacaaaa
     2221 acaaggtggc tttttccttg gatacctaat tggtcgattt gtttagcaaa agattcactt
     2281 gtaggatttt tcccttcaat tgctaaggca tagacgtatt cttgttcttt gattttcgct
     2341 aagatccgtt ccccttcttt ttcttttact tgtctcattt gcgcttcgct taaattctcc
     2401 ggggcttttt cgtcaggaac ttcgatcaat tcgatttttg cataggcatt cagccgtttg
     2461 acatactcat tgataccttg gatcaggtat ttttctttta attttcctac tgagataatt
     2521 ttgatattca tgttttctct cctttggtct tcatgctcac tttgtttatt ataatcaatt
     2581 ttttcgtcta tatgtttatt ttggctattt ttgttaaaaa gatttcccca acagttatgc
     2641 acaacatcca caggtttatc cacagaaaac agtgttattt taccttattt catcacattc
     2701 attaggcaaa catacgtttt ataagaattt atccacttta tacacaggct gtggatatgt
     2761 tttatccctt atttttccaa tcctatccgc gtagttttac acagtatctg tggaaaaaga
     2821 aaatcaaata ttttcatctg aaaacaatga tttttcctaa agtttttcat aaaaagttca
     2881 cagtctcaaa gtagactaaa gtcaacaaat aagactatac taacagtaga taaatgaatg
     2941 taattggagg aggaacacat ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa
     3001 aataatagcc tctggcgtaa gttaggtctg gggcttgttg gagggatcgt tggaggacta
     3061 ctgacagctg gcatttttta tgcagcgatg ggcactggca acaatagttc tactgctaca
     3121 agcggcaatc agaattctgc cggtgaaaca gttgtggaaa atgtcaaagt caatgtagat
     3181 tctgatatca ccaaagcggt agataaagtt caagacgctg tcgtttctgt tatcaactta
     3241 caaagtcaaa accaatcaag cggttttggc ggactattcg ggcagcaaga agaaagtagc
     3301 agctcagatg atagtaattt ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa
     3361 gacgggaata ctgcttatgt agtcacgaat aatcacgtag tagatggcca gcaaggtctt
     3421 gaagtcttga tgaaagacgg tacgaaggta aaagctgaat tagttggaac agatgcttat
     3481 tcagatttag cggtgttgaa gatcaattca gacaaagtcg aaacagttgc ctcttttggt
     3541 gactcaagtg ctctgaaggt tggtgagcct gcgattgcga ttggttcccc actaggatct
     3601 gaatatgcaa attctgtcac ttcaggaatc atttcttcct tgaaccgaca agtgacaagt
     3661 acgaatgaat cgaacgaaac agtcaatatc aatgcgatcc aaacagatgc agcaatcaac
     3721 cctggaaatt ctggtggtcc tttagttaat atcgaaggac aagttatcgg tatcaactca
     3781 agtaagatcg caagtacttc tgaatctact tctaatgtaa gcgtagaagg aatgggattt
     3841 gctattccaa gtaacgatgt agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg
     3901 acacgtccag ctctaggtat cacaatggtc gacttatctg ccgtttctac tcagcaacaa
     3961 gaacaaatct tgaagattcc ttcatctgta actaacggtg tcatcgtgac aaatgtccaa
     4021 acagctacac cggctgaaaa agctggattg aaacaatacg acgtcattac taagatcgat
     4081 gacaaagaag tttcttctgg tgtagaactt caatctgctt tgtataagaa aaaagtggga
     4141 gattctgtca aaatcactta ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg
     4201 acgatcgatc aatcggcatt aaaacaaaat caaaatagtg aaaattaata aaaaagaggt
     4261 tgtgacaaag acttgtcaca acctctttat tttggaaatg cttctccgac tacggcatcc
     4321 tcaggcaata ctaagattcc tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg
     4381 gaacagatca ttccatagct atctacccct cgcaaagttc ctggccagat catcattcca
     4441 tctggcatca tagctcctgg ttttgcaacg acaacttttt gtccagcttc gatattgggt
     4501 gcaccgcaaa cgatttgcaa tactgtacca ttgtctactt ctgtctcagt aatcgataaa
     4561 tggtcagaat caggatgtgg gacgcatgtt tttacgtacc cgacaacgat cttcggttca
     4621 tgatcagctt caagctgttc ttcgaatcct gcttctttga tcaatcgatt caatttcgct
     4681 aattcctcat ctgttaagct tacttggccg ttcccttcga ttgttagatg atcggagata
     4741 tggaaaaagt tccaagcaac aacttgacct tgttcatctt tgatccgagc aacattccat
     4801 ttgcgttcaa cagtgttttc tgttccttta tcgtcggcta taataaccat caaggtgtct
     4861 ccgacgtgtg ctttgttata agcaaaaatc atgttctttc ctccattatc ctttgctgag
     4921 atttatctta acaaaaaatt gcattcactg catgaaattt ttttcatgct ttatttccga
     4981 agaagcctct tctggaacac catttattcg taacaaggga ctgtgacaag acttttgtca
     5041 cagtcccttt actgttttag ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa
     5101 ttacttggct gttcttcttc gtcagaaacg atcaagacat cacaaggtga atgacgtatc
     5161 acgtagctgg ctacacttcc tgtcataaag cgttcgactg catttagacc tgactgccca
     5221 accatgatta gatcgatctc atattttttt ggcaatttga ctgccatgga ttcacgaaca
     5281 gaaccatatg caataattcc ttctatattt gtgtaatcaa cagatttccc atattcttta
     5341 cattcttcga tcatttcctt ggctgtttca gtttgctgat cgactagatt ttgatcaata
     5401 ggtgcatagc ccataaagtt ataaaattgc tgatcgatta cgtttgctac atatactttc
     5461 ccattattac ggcgagccac ttctaccgct tttttgaaag cagcaagtgc ttgttcacta
     5521 ccgtcgatac ctactaaaat attcttatat tcttgattca tcttgacccc tcctaacaga
     5581 tgtcagcctt ttgttacact ttctaggaac gattcgattt cctgttttgt tttacgatct
     5641 ttattcacta gacgacctaa ttcttttccg tcttggatca cgacaaaact tgggatacca
     5701 aaaatgttcc attcagcggc tacatctatg tacttgtcac ggtctacttc aataaattgc
     5761 caatcagaaa agtcattttc aatttcaggc atttgcggtt tgataaaacg gcagtctccg
     5821 caccagccgg cagtaaagaa aaatacactc tttccagttt ctacgtagcc tgctaattct
     5881 tctaaactat ttgggataat cattctttgt cctctacttt ctgtttatgt atttttcaat
     5941 taaattatac acctttcttt cgaactaaca aacattgatc ctctttttat aaaaaaaatc
     6001 tggcaaaaag caatcttttg ccagatttta tagtttcact atcgcattat tttccgtata
     6061 cgatcgtatt tactgttgat ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg
     6121 cagcttcaaa atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta
     6181 cgccaatcac tgtacttggg attccttcgt tttgtgtatg agcagcacct gcatctgtac
     6241 cgccttttga aacaaagtat tgataaggga tattgtttgt ttcagcgata tccagcaagt
     6301 actcgcgcaa acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt
     6361 ctcctaaatg tccgaatgtg ccatttttcg tcactgtatc atctgctgca gagcagtcta
     6421 ctgcaaagaa cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta
     6481 cttcttcctg gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgcaacg
     6541 cttctaaagc ttctaatacc attgtacagc catagcggtt gtcccatgat ttactgataa
     6601 tattctttcc gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa
     6661 ggacaccgta actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatcgg
     6721 ttacttctac tgatttttgt ccgcttgttc ctcgcaataa atgtggggga acggaagaag
     6781 aaatacatgg ataattctta ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa
     6841 cgtaaggatt ccagccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg
     6901 tcagcatgaa tccgacttca tccatatgtg ctgcgaccat tacacggggt gcatccgctt
     6961 cttcatgatg gcgcagacca aaaatgccgc ccaaaccatc taactgtaac tcatctacca
     7021 gcggttccat atgactttcc atataagcgc gaacatcttg ttcaaaaccg cttgttcctt
     7081 gtaattctgt taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta
     7141 aatttcagac taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc
     7201 tgcagaccga tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag
     7261 aaaatatgag agtgtggtaa aataagcaca aggagtaaca agtgagaaga aggaggtatg
     7321 tccacatgta tgaagaaagc gaactaagct attttaaagg cgggttagca gttggaatcg
     7381 gtctaggatt attgagcggg gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt
     7441 cagcagacca agtacttgaa aatatcaagt catcttttct aaaagaaggt gcaatcgaag
     7501 gttcttggat ctcttttgaa aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt
     7561 atcggggagg tctttctcga atagaagatg gcgaaactgt ttattatgaa tttttagcag
     7621 atgctcatac aggaaccgta ttagaaattt caagaaaaaa aggtgcaaca gattaatacg
     7681 tcaaaaaagt gagagaacga atacgcaccc actattcaaa agagaggtat catgtccttc
     7741 ttttgtagtg ggtggttttt tagatagagc tttccaaaca tatattccag tattttcttt
     7801 ttaaataaat gatatttagc gggaaaaact agcatttcct ttgattatga ggtattttta
     7861 atttagcttt tgttttctat agttaaggag agtgaaaaat ggaacaacaa gaaaaaattg
     7921 ctgttctgat tccttgttac aatgaggaag caacaatttc aacagtcatt gcagacttta
     7981 aacgagaatt accagaagca gatatttatg tctatgataa taattcgacc gatcgaacat
     8041 acgaattggc cgttcaaggt ggagcaatcg taaaaaaaga acctcgtcaa ggaaaaggaa
     8101 atgtgatccg ccaaatgttc tttgatatcg atgccgacta ttatttaatg gtagatggcg
     8161 atgatactta tcctgcagaa gctgtacatg gtttgctcga gaaattacgt tctggtgaag
     8221 cagatatggt cattggtgat cgcctatcaa atggaaccta ctttgatgag aataagagac
     8281 ctttccacga ttttggaaat aaccttgtta gaaatacaat tactagaatg tataaaacaa
     8341 aaattcttga tgtcatgacc ggctatcgag gattcaatcg tatctttgtc aaaagttttc
     8401 ctattatgag ttctggtttt caaatcgaaa cggaattgac gatccatgct ttagataaaa
     8461 aattcaagtt cgttgaaatc ccaatcgatt atcgtgatcg tcctgaaggc agcgaatcaa
     8521 aattgaatac attctctgac ggattcaaag tgatcatgat gattattaaa atgtttaaag
     8581 attataagcc tttgcttttc tttgggattt ggaccatctt cttcttttta tttggattgg
     8641 tcacggggat tcctgtgatt cgcgaatata tgctgacaag ctttattaca cgcatccctt
     8701 ctgcgatttt aagtactgga ttgatgactt tagcacttct ttcattagta acagggttga
     8761 ttttggatac agtcgtcacg aacgctaaga aagaatatga attgaatcta tatcatgttt
     8821 atcatgaata ccacagtaga aagtaaaaaa aggatcaaga ctgcaaaaat aatttgcaat
     8881 tcttgatcct ctttttaatt aattatttcg ccaaacgata gatggcatct gcatagattg
     8941 ctgttgctct gaacaagtca tcaagactca taaattcgtt tgcttgatgc attgtatcag
     9001 tatatcctgg gaacatcgct ccataagcaa cgccacgttt caacaaacgt ccgtaagttc
     9061 cgccaccgat gatttgttcg tagccttttt ctccagtgtg gtcttcatag acttgcaata
     9121 atgtagccac caatggatca tccattggta cgtaatgtgg ttccattacg cgagcacctc
     9181 gagtaactgt tgcgccttct tgccccactg tttgttcgat gtcactttgc aatccgtcta
     9241 ctgttacacc ttttggaaaa cggaaattca tattgatgaa gttcgcttcc tctgtaccat
     9301 tttcttcaaa agcaaatagt ccagcattca ttgtcaattt gcccattttt tcatcttcat
     9361 aagcgactcc tagtttttct ccataaaaat cttcatggac ataagcagct gctacatgga
     9421 taaattgttt agcagatcct aggaattcat agttatctaa gaacaatgcc aagaaagaac
     9481 ctgcattgat accagattgt gggcttgcac catgtgcgcc tttaccaact aattcgattt
     9541 ttacatacgt attgtccgct tcaatcgttc cgcttactgg atttgcttcg atgaattggt
     9601 aaaatgcttc ttccatcgcg atagctgcat ctgcacttgg tacttgaagt gctgcagttg
     9661 ctgttcctgg caccatattt tcgcgaagtc cggaaacaaa agatttcaag acataatctc
     9721 ctgcattgtc gcctttgaag cgtaaagcta aagaaacatt tcctttttca ccattgatga
     9781 ttgggaattc agcatctggt gagaaaccaa aatctggttc ttcttcatgt tcgaaataat
     9841 aagccatatc gccccagccg ctttcttcat cgctgccgac tacgaagcga actttttttg
     9901 atagtttcaa gtcaagttct ttaatgatct tcatcgcata gtaagctgcc atacttggac
     9961 ctttgtcatc gcttgatccg cgtgcaaaga tctttccatc cttgattact ggttcataag
    10021 gatctgtgtc ccagccatct cctgcaggaa cgacatccat gtgtccaaat atccctagcg
    10081 tttcgtctcc ttcgccaaga tcaatatgcc ctgcatagtt atcgacattt tttactttaa
    10141 atccatcacg ttcgccataa gctaacatat gtttcaatgc gtcgcgaggt ccaggtccaa
    10201 atggtgcatc tggtgttact tttgagtcat cgcgctcgct gttgacacgt aaaagatttt
    10261 tcaagtcttc aagcaagtct tctttacgtg cttctacttc tttttgccaa tcaattgtca
    10321 taaagttaat cctccactta tttaagtctt cgtgttcatg ataccacaaa ttacagaaaa
    10381 aaatagttca aaaaatctga caattccttt tcttatctgt ttttctctaa aaaaaatata
    10441 cgctataatg agtaaaagga ggtttttaga tgctgataaa cgaacaacta gtgaaaagag
    10501 tcatcacacc tcgtccaaaa atttcacata aaggaacatt tggacgtatc gttttgattg
    10561 gcggcaatga acaatatggc ggagcaatca ttatgagtgc tttagctgcc gtacacagcg
    10621 gagcgggtct cacgactgta gtaactgatg aaaaaaatcg gagtgctctt cacagtcatc
    10681 tgccagaagc aatgattgta gtttggacgg atactgacca gtttctttcc gtaataaagc
    10741 aagcagatgt actcttgatt ggtcccggat tgggtacttc ttctaagagt ttggaacgtt
    10801 tcaaacaagt actagaaaac caatcaaaag aacaatggct ggtgattgat ggctctgctt
    10861 taagtttact tgctgctaat catcttttgc taccctatcc cgaacaaact gtacttacac
    10921 ctcatcaaat ggagtggcaa cggttgagtt ctctaccgat acaagaacaa cgggataaag
    10981 aaaacaaaaa gaagcaacag gaaatcggtg ctgtgctcgt tttgaaaagt catgagacaa
    11041 aaatttatgg tcaccagcac ttctacacca atcctttagg ttctgctgct atggcaacgg
    11101 gcggcatggg tgacacttta gctgggatga tcacaggatt tttagcccag tttcctcaaa
    11161 atgaccaaac gattgcagca gctatttatc ttcatagttt cattggagac cagctggcaa
    11221 aagaacaata tattgttttg ccgacacaaa tcagcgaagc acttccgaaa tggatgaatt
    11281 atttttgtaa aaaatcaatg gaaaacgagg catgagtcat ggagaaattt acaggttatg
    11341 tgcgagaacc tttttattat gaaacagatc aaatggggat tattcatcac agcaattata
    11401 tccgctggtt tgaagaggct cgggtagcct tattggtaca tctagggttt ccctataaaa
    11461 aaatcgaaga atctgggatc atcatccccg ttctggaagt aaattgcagc tataaagaaa
    11521 tgatccatta cggagaaaaa gtgagcatcc atccaactat ccaaaagtat acggggacac
    11581 ggttggattt tagctacgaa attgtgggaa ctgaagacaa aaaattaaaa actactggca
    11641 gtagtaaaca ctgtttttta ctttctgatt cgcaacgatt agtgaagctg agcaaagtca
    11701 atccggagtt agatcagtta ttccaagcgt acgcttcttt ggatgatgta aattcttaat
    11761 aaagaccgtc taagcatttt tcttgtattg tgtcgagtag ttcgtataat gaaaagaaaa
    11821 aagcaggtga tccccatgta tgtcgttaaa gtaatgcacg gttacatcga taaaactggc
    11881 tgtcgcactc gggaaaaaaa cttagacaat ctgctcattt tcaaagacaa aaaagaatcc
    11941 gaagcttttg ctaaaagaat cggcggtcgt gtcaaaccga tccaagaggt acgaccagat
    12001 tgattcaata tttttctttt cgaggctcta ataggcatgc ctatcagggc tttttttgca
    12061 gactgtgttt ttatgcccac acgatttgta agcgatttat gttataatag attatatctg
    12121 catagaaagg aaggttaaat ggttatcaat aatttgcaac agcaagctta tgaatctata
    12181 cgtaaacaaa tcatttattc cgagttagca cctggaagta aaatctccga taaagtgtta
    12241 gaagaacaac tagctatcgg gcgtacaccg attcaagaag cactgatcca actgcgcaat
    12301 caagagcttg taaaaaccat tccacagtcc ggcacttatg tttcaaaaat cgatatccga
    12361 tctgcttcat tagcccgtta tacgcgggaa aagttggaaa aaccgatcct gcaagaatgc
    12421 agtgcgaaaa tgaccaagca agatcaagct aaactagaaa aaatactaga acaaaccgat
    12481 caggcagtaa tcgctcaaaa taaaaaagct ttttttgatt tggatactga ttttcaccga
    12541 acttgttatg aaattgctgg aaaacgagaa atttgggatt ggctagaaag ttatagtacg
    12601 cacttgaacc gtttcagatg gttacgtttg acgatttcag aattagactg gggtcgagta
    12661 ttagatgaac atcagacaat gcttcaatca atgattgatc ataactttga tgaagtcgga
    12721 tttctttgca cgatgcattt acatatgatt atcgaggagc aagaatacgt gattcataat
    12781 taccccgatt attttgaaga tattcaggac agacccatca aataagaggt tttggaatca
    12841 gcgttttgac ctgagtatta gagaaagaaa cggcaaaata gttcctcata ttttgccgtt
    12901 ttttttgagc taataccgag ggtcagctga ttgcacaccg tttattgaga ggttgtgaaa
    12961 aagctgccct gcatcaagta ataagaacag ccaagtaaaa atagcttctt atgtttttaa
    13021 cttggctgga cttattaccg cagatgcaag cttttgaaca ccgttttcca agaggtcgtg
    13081 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
    13141 ttgaatattt tgacttgtga ccgaggagtt gacaggagaa catcgtttat tcggaaaaaa
    13201 aggactgtga cacgactttt gtcacagtct ctttatttgt aatctaaatc atactccgta
    13261 catctttttt ctattttttt tatttcttct aagatccctt ggctacgaat ttcattatat
    13321 ggccaaagta agactgcctc taatacataa ggcgaaaaat cgctttgatt catctctttt
    13381 gaaaaactca tcgctgctat ctcgctcaat gcacgaatgt ttgattgatg tgtataaaaa
    13441 ggcaaccgcc acagcacaat tttttcgctt tgcgtataaa ttcccttaac agaagcttcg
    13501 atgtggtgaa atagttcatg tccaataatc aaagcttgcg ctgtttcttt agagatcgaa
    13561 gctagtcctg aacattttaa tttttctaac ggctccgtca tcaatcgaat cccttcatct
    13621 tctgcataag ttgcaaaagt gattcgttcg tttatttgct ctgattcaca ggaaacgatt
    13681 ggaacagcca tttgttcaca aatttctcct acatcggcaa aagggtactt ttttttgatg
    13741 ctacatgccg tttcgatacc acaatcgatt gattgttgta taagaatttt ctgcttggac
    13801 aaaggaatct ttttccaaaa taaatctcgc cgaaactggt agaatcccca aatttcttcg
    13861 gggatctgtt gtaattcgtt tagtaactct tctaacttat gtttgtttga tatttccggc
    13921 aacgacgatc acttcttcct ccggttccat agtagatact tccagatcca tcaaaagctg
    13981 tagttgttcc agatccgtcg ctgtaaaatc agagactctt gctcctaagc agagataaaa
    14041 ttctggtcgg gcaatcaatt cagtttgata aactgccata tcttcccaaa gttgttcgac
    14101 agctgtcata taattggctg cagttgtttt catacatgaa gcatgtccac gttggacctt
    14161 gataaatcct ttttgatcga tgatccgaag acttccagca ttttttgaac gattctgttc
    14221 gccgcataca taaaagaaag gtgtattttc caataagcaa acttcatttt tatttaatcg
    14281 catatcctct gcagcaagct ctagtgcttc ttctgttgtg atctccttgg ttagatcggt
    14341 tgctttcact tccgtagaac cagtagcgat agccgttact tttgaagtct gtggatcgat
    14401 ttctacgtgg acttcgatcg attctggtgt agcgcctgat tcaatcgctt tattcatcgc
    14461 ttcatttttc aacgatcgta tatcttcttt acttggcgaa gggatgatac gttctacaac
    14521 atctcggacc attgcaagtg ctacaccaat agaagaaatc acttcggcgt tttcaggaat
    14581 cgaatatttc acacccattt tattactaaa gtaagtaatc aatgaggcag cgccgccccc
    14641 tacacctaca agagaaatct gatctttctc caagtgatac ttgtctgcca attctaaaat
    14701 caccggttca atcttagcat aggatttttc catgatctgt tctgcgatgt cttcaaccgt
    14761 cgtatgacaa taatcagcta atgcttggat ggcttttcgt gcagatggga catttccata
    14821 agaaaaatgt tcttcttgca ctaatccaag gacattggct gcacatgtat ttgtaatagt
    14881 cacttcttct ccgtcttcca tcactacctt gacgtaatca gcagggtctc cttctttagg
    14941 agagaaaaat tcaaccttag gacctttgat tttttctgtc tccgtaaata ctgcataatc
    15001 tagaccggca atatgtgcag aacgtggacc gacatcgatg attcctgact gattagcccg
    15061 taccatcgaa ccgcctgcca cacctagaac ccgaacatct aatgaagaga tatatgttgg
    15121 gtggccacct acaattgaat aatcgattgc tggacgtcca tttttaatga ccccgatatt
    15181 ggttgttgtt ccgcctactt caaaataaac accattagac gctctcaagt acatcaaaga
    15241 gcccatgact gatgctgctg gaccggaaag catggttaaa acaggtcgtt ttttcatttc
    15301 attgatttcc attactccgc cgtctccgcg cataatcatc aaagaaacat tgacccctgc
    15361 ttctcgtacg gaatcttctg tagaggttgc cgtatctagc atttttggta aaatagatgc
    15421 attgattgct gctgtccgag ttcttcttgt caatccatat aattttgtga tgtcagaagc
    15481 catcgttgta gggatcgatt ttaccgatgc tgcttcataa acaactcgtt ctggttcccc
    15541 attatctact ccaaatgcca tagaagaaac caagacctcc gctctttctc tttctaaaga
    15601 agaaatcgtt tctgaaacac ggtctacatt taagtgtttg acaggtaaga atgcattgac
    15661 aatctctatt tttttcttat tccctaaatc gatatcgttc aatctagttt gtcgttttgc
    15721 taaaaaacct tctaatccac cttttgccat cccgattacg ccgacttttg ctacgtctcc
    15781 ttcaatcagt gcattagtcg cttgtgtggt agaatgtgca acaaacacaa catcttctgg
    15841 actgatatta ttttccagca aacaattttg aaaagactga acgacacctg cggctactcc
    15901 acggacatca tcgtgggttg ttttcactga agattttccg atgatttcgt gcgtcgcatt
    15961 atcgattgcg actgctttcg tatgtgtacc gccaacatca attcccattc gcacaaaacg
    16021 tttcattttc ttttcctcca ttcatctaat cgtttttatc gacgttttgt ttactaggcg
    16081 ttcccgtaca ataagaacgt gatcaggcat aagatcgctc cgatgatcca accagttggg
    16141 atggatttct tcatatattc tcgagaagtt acttttgtat aacctaatcc ccaagcaacc
    16201 caagattgtg taatatctaa atgctgagga gcaatcgttg tgattgcaaa taacgggaac
    16261 aaaaaggctg gcgacatcgt tggattaaca gctaatacga cagctaaaat agctgagcct
    16321 gaaccgacta gattcattgg accgcggaaa aatcctagtg gtgtcaatat agcaaatacg
    16381 attgccaaga ctaatggtga ttgcggtatc caatctccaa aaatagctga aaaataagga
    16441 gaagcatacg cagctgaatt attgaacatc gctaaggtca tcaagaaacc aaccattggc
    16501 gcaacatcta ctgcgccatc tgtaaataat ttcgcaaact gacgacagat ttctttataa
    16561 cctccgcgca attttccagt aaacagcaat gcccagatac ctgctagaat aaaacctaag
    16621 atgattggaa gatctaaaag aacgacccct aaaactggta ataatacggc taaccatgag
    16681 atcattggtg cgtcgccacc ttcagctggt acgtttgctg ccattgcata gcgtttcttt
    16741 ttgttcattg atatattagc gaccgttagt accacaacta agcttacaat catcccaatc
    16801 ataccaattt ggaaatagtc attataagta tagctttcag ccgctggatt gaaaccagca
    16861 taaatcgttt gatattgttt gaaattgacg atattagcaa agattccagc cataatcgag
    16921 cccataaatg agaacagtgc aaccggtact gaaataccta atgacatcaa gattggcaac
    16981 acaataactg caatagaaat cactggtccg attcctgtca tcgacatgaa tataacagcg
    17041 gttacaatac atagtaagct catcgtgatt cttggcttgt cccctccaag ttcaactacc
    17101 tttcgaatca gggtagctgc gattccgcta tctactagaa cacgcccgaa aaaggcacca
    17161 aagaagacat ttaccaagat cgatttggcg tactcagctg gccccgtcgt atagatatgc
    17221 gtcaacacat caattactcc ttgcccttcc atcgccgggt ttggtgaaaa cgtgtttccg
    17281 actagcgcta atcccatcca tacagtagcc atgatggcaa agccgaccat caagttgtag
    17341 ccttttacac agtaccagac catgaaaaag aatgagataa ctaacaaaat accaataata
    17401 atatcactca tgttattcct ccgtccattt tgtttttctg ataagcaaac taatatatca
    17461 gtcaaactca ttctaactta tgtagttagc gctttcatta aagggtgacc ataaatgttc
    17521 agttttcaat taaaaaacct taattatgct attttagact tatacgttat tgtcctgttt
    17581 catttgatgg aggattgtaa ataaaaaaag aggaaagaac ataacttttc gttatcttct
    17641 ttcctcttta taattgttta gcttatttag ccaaagcttc ttttgcttgg tcagccaatg
    17701 cagtaaatgc tgctgcatcg ttaacagcca agtcagctaa cattttacgg ttgatgtcga
    17761 tttcagcaac tttcaaaccg tgcattaatt ttgagtagct caagccattc atacgagctg
    17821 ctgcgttgat acgagcaatc cacaatttac ggaagtcgcg tttcttttga cgacgatcgc
    17881 gatatgcgta gttgtatgaa ttcattactt gttcttttgc tgttttaaat aaagtgtgtt
    17941 ttgaaccgta gtaacctttt gctaatttta gcactctttt acgacgttta cgagttactg
    18001 ttccaccttt aacacgtgcc atgtataatt cctcctaata aaaataaatc ttcagattct
    18061 gttatttgta cttcttattt catttttgct agttgttggc gaatacgttt gaagtcgcca
    18121 ctagaaacca tgcttgcttt acgcaattga cggcgttgtt tttttgtttt gccgtggaaa
    18181 cggtgacttg taaacgcgcg gaagcgtttc aatccgcctt taccagtacg tttgaaacgt
    18241 tttgctgatc cgcggtgtgt tttttgtttt ggcatgacta tatttcctcc tcaaaatctt
    18301 tcgttatccg actatcggct gtccaacagc cagttagtca ttcaaattac ttactgtcgt
    18361 ttttcggtgc cagcgtcaag aacatgctgc gtccgtccat tttcgctttt tgttccactg
    18421 ttgcaatatc cgcagtttct tcagctaagc gattcaagac tttttgacca atttctttat
    18481 gggtaatggc acggcctttg aaacggatag aagctttcac tttatcgccc ttttccaaga
    18541 acttacgtgc attacgaagt tttgtattga agtcattcaa gtcgatcgta ggacttaagc
    18601 gaacttcttt tacattgatc actttttgtt ttttacgcgc ttcgcggtct ttcttttgtt
    18661 gttcgaaacg gtatttaccg tagtccatga ttcgcgcaac tggtggtttc gcaccagggg
    18721 caactaacac aagatccaag tttgcttgtt ctgcaatatt caatgcttct gcttttgtct
    18781 taactcctaa ttgctcacca tctgaaccga tcaatcgtaa ctctcgtgca cgaatgccgt
    18841 cgttaaccat catatccttt gctatggtca ttcacctcca aatattatga gagaaagaaa
    18901 agcttaccga ataaacggca ataaaaaaac gagttctctt acagaacccg cgcgtacttc
    18961 acctaataga accagtgaaa actatctagc ccagtgacat tatcaactaa ggcgagaagc
    19021 gggtgcttct gcttttattt ctcaaccatc atagtatagc aaaaactaat ctttttgtca
    19081 atgtttttca tacagtttgt cttccttttc tgaaaatgat tagattagca gactttcttg
    19141 cagtgattcc cgcttctttg ctatactaaa gaaaaacgag gtgaacccac atgaaaaaaa
    19201 tcgaacctat tccaccttct cttcctcttc ttacaaaggg agagttttat ttagaagatg
    19261 aggagatctt ctctggtctt ctgatcaacg ggggtgacca aagctatctc gatgccaaaa
    19321 atatggtatt aagagagagt catatccaaa aacttacgat gcaaaaaaca gttttacgtc
    19381 attttgaatg cagtaatgtc atttttgaaa aatgtgattt ttccaatctg gagtggattg
    19441 gtggcagttt ccaccaagtc gtgtttcatc aatgtaaatt aacaggtacg aattttgccg
    19501 aaagttatct tcgagactgt actttcactg attgtattgc ttcgatggct tcctttagta
    19561 gtacaaactt gaaagctgtc tccttcgatc attgtcaatt agaagacagc gaattttatg
    19621 aggtcacttg gaaaaacctt tttttgtctg acaatcagtt gaccggttcg aactggtttc
    19681 gaacttctct gaagggatta gactttacga ctaatttctt cacagcaatc gctttatctc
    19741 aagattcttt ggcgggacta atcgtcaacc aagagcaagc acttgtgatc gcccaagccc
    19801 ttggtttgca gatcaaagac tagatcaacg aatccgccgc cacagtgcaa taagcatcca
    19861 aatagataag accaaactga tgatcaaggc gattccccat ccgaaaacgg aatgggtaaa
    19921 gggtaactgg agattcattc cgaagaatcc tgtcacaata gttggaacgg tcaagatcag
    19981 cgaccagacc gtcaaaaatt tcatcgtgtc attcaagttg ttattcagca aattattata
    20041 agtgccagag agctggtcta aaatctgcga agctaagttc gtcatttcaa ctgcttgtcg
    20101 tgcttcgatc agcgcatcgt ccaactcttc tttttccttt tctgacaact tgcgataaat
    20161 agccaatgcc ttgatttgtt ctaaaagtac tacattttgt tttgttcctg taactaagta
    20221 aaccaaaccg atctctaaat ctgaaagttg cagcagattc ttgttcgttg ttttttctct
    20281 taatttcaga ttaagccgct gacgttcact attcacttcc tcgattaaag gaaaaaagga
    20341 atcagaaatc aaaaacaatg tacgaaacaa taaactgtat acactcatgt ctggcattcg
    20401 ttccaaaagc gatcgaatta gtcgaatcac gtactcggta ttcggcgtgg taaatgtaaa
    20461 caaaccgtct tcttttaaaa taaaagcgat aggactagtt tcataatgat tatcgacttt
    20521 ttgctggtgc ggaacatttg atacaagcag taacgcttca tcaaaagaat cgtactctac
    20581 gcgcgcccgt tcatttttat ctagagaata agtcagcatc tcgtcactaa tgccatattc
    20641 ttcttttaag tttttaacag ctagcacatc gttttgctgt acattgatcc aaaaattacg
    20701 gtcttgatta aaatagtgtt tttcccgcat actctatcct cctgacattt gtttccagta
    20761 taaagaaaaa agtcgctcaa ataaaggttt accctttatt tgagcaactg taaatcaaat
    20821 ggctactttg gtttggcgaa tctctgctaa tttttcttcg atcattttca agacttcttc
    20881 ttttgcttga ggatcttcca caaaattata gcggtctcca tcgatttgga tttttggact
    20941 ttctttgtat tcttcatacc attgatcgta acgtctgttc aattctttgt aataatcgta
    21001 tagacttgga tcaaaggaca attgttcata cgaacgacca cgtttttcga tacgctccaa
    21061 catcgtatca aaagatacac gaatatgaac taataaatcc ggatgttttt tatgggcagc
    21121 atagggaagt tcttccatca tgttttccag cagttcatca tacacacgaa cttctgtttc
    21181 tgttgctctt cctagatctg catttagatg gaaaagcagt gaatcttcat aaatcgaacg
    21241 gtccaataca ttattgtcat ctttcattgc ttgtttgatg cttgcaaaac gtttattcaa
    21301 aaaataaatt tgcagcaaaa aagcatactg ttcagggttt gcataaaata agggcaagac
    21361 ttcattatca tcaatcgatt cataaaatgc ctgactatca aagtgttcag ccatcatttc
    21421 tgttaaactt gattttccgg caccaattgt acctgctaaa acgatcacgc tcatttacct
    21481 ccagctcact tcatcactaa atattgtgtt taactccaac ctttttaaat atacccccaa
    21541 tatctagtag tgtcaattgt atttttttct agtcaaagaa gaaaaaaagt caaaaaattc
    21601 ttaagaaaaa agtgtttttt ctttagaaaa cgtattctta gttttacttc tgagatggtt
    21661 ttagtaaaat aaaggaggaa tgttttttga gacccttagt ggacaaaaaa attcgtgaag
    21721 cagcgcaact cgttttgggc gctttatttt ttgcttaaaa atcactttta cgtgagaagg
    21781 agttagatta tggcaaacta tggccaagga gaatcaagcg gaaagatcat attgatgggc
    21841 gagcacgcgg ttgtttatgg agaaccagcg attgcctttc ctttctatgc aacaaaagtc
    21901 accgcattcc ttgaagagct ggatgcaatg gacgatcaac tggtttcttc ctactattca
    21961 ggaaatttag ccgaagctcc tcatgcatta aaaaatatca aaaaattatt cattcactta
    22021 aaaaaacagc atgacatcca aaaaaacttg caactgacca ttgaaagcac gattcctgct
    22081 gaacgtggaa tgggatcaag cgctgcagtc gccacagcag tcactcgtgc tttttatgat
    22141 tacttagcat ttcctttgtc tcgtgaaata ctattagaaa atgtccagct ttcggaaaaa
    22201 atcgcccatg gtaatcctag tggaatcgat gcagccgcta ctagcagctt gcagccgatt
    22261 tattttacaa aagggcatcc tttcgactac ttttctttga acatcgatgc ttttttgatt
    22321 gtcgctgata caggaatcaa aggacaaaca agagaagccg tcaaagatgt cgctcacctc
    22381 tttgaaaccc agcctcatga aactggacaa atgatccaaa aattaggata cttgacgaag
    22441 caagcaaaac aagcaatcat tgaaaattca ccagaaacgt tggcacagac aatggatgaa
    22501 tcacaatcac ttctggaaaa gctgacaatc agcaatgatt ttcttaatct attgatccaa
    22561 acagcaaaag ataccggggc attgggtgcc aaattaactg gcggtggacg cggtggctgt
    22621 atgattgcat tagcacaaac aaaaacaaaa gcccaagaaa tcagtcaagc acttgaagat
    22681 gcgggtgctg ctgaaacttg gatacaagga ttaggagtac atacctatgt ttaaaggcaa
    22741 agcacgcgca tataccaaca ttgctctaat caaatattgg ggtaagaaaa atgaagaact
    22801 catccttcca atgaacaaca gcctttcgtt aacattggat gcattttata cagagacaga
    22861 agtcatcttt tctgatagct atatggtaga tgaattttat ctagatggca ccttgcaaga
    22921 cgaaaaagca acaaaaaaag tcagtcagtt tcttgacctt tttcgtaaag aagccggcct
    22981 ctcactaaaa gcttcagtaa tcagtcagaa tttcgttcct accgcagctg gattagcctc
    23041 ctctgccagc gggctagctg ctttagcagg cgcttgcaat actgctctta agcttggatt
    23101 agatgatctc tctctttcaa gatttgctcg acgcgggtct ggttcagctt gccgaagtat
    23161 tttcggtggt ttcgtcgaat gggaaaaagg ccatgacgac ttaagttctt acgctaagcc
    23221 agtcccttcc gattctttcg aagacgattt agcaatggtt ttcgttttga tcaacgacca
    23281 gaaaaaagaa gtgtccagca gaaatgggat gcgtcggaca gtcgaaacat ccagttttta
    23341 tcaaggctgg ttagattccg ttgaagggga tctatatcaa ttgaaacaag caatcaaaac
    23401 aaaagatttc caacttctcg gagaaacgat ggaaagaaac ggactaaaaa tgcatggaac
    23461 gactttagct gcccagcctc cattcactta ctggtctcct gacagcttaa aagcgatgga
    23521 tgcggtgcga caattgagaa agcaaggcat tccatgctac tttacgatgg atgctggccc
    23581 taatgtcaaa gttttagtgg aaaacagcca tttatctgag gtacaagaaa catttaccaa
    23641 attatttagc aaagaacaag taattactgc acatgctggt ccaggaattg cgattattga
    23701 ataaagggag atttttatga ttgaagtatc tgcaccaggc aaactctata ttgccggaga
    23761 atatgcagtt gttgaaacag gccatccagc agttatcgct gcagtcgatc aattcgtgac
    23821 agtcactgta gaatctgcac gaaaagtcgg aagtatccaa tctgcacaat atagtgggat
    23881 gcctgtacgt tggacaagac gcaacggaga attagtttta gatattcggg aaaatccttt
    23941 tcattatatc cttgctgcta ttcgcttgac tgaaaagtat gcacaagaaa aaaacattct
    24001 tttatccttt tatgatctga aagtaacaag tgagttagac agttcaaatg gccggaaata
    24061 tggattgggt tctagcggtg ccgtgaccgt tgcaacggtc aaagccttga atgtttttta
    24121 cgcgttgaat ttatctcagt tggagatttt caagattgca gcactagcca atttagcagt
    24181 tcaagataat ggttcctgcg gcgacatcgc tgctagctgt tatggtggct ggatcgcttt
    24241 ctcaaccttc gaccatcctt ggctccaaga acaagaaact cagcattcta tcagtgagtt
    24301 acttgccctg gattggccag gtctatccat tgagccattg attgctcctg aagatttacg
    24361 tttattgatt ggttggacgg gtagccctgc ctctacttct gatttggtcg atcaagttca
    24421 ccgttcgaga gaagataaaa tggtggctta tcagcttttc ttaaaaaata gtacagaatg
    24481 tgtcaacgaa atgatcaaag ggtttaaaga aaataatgta acgttgatcc aacagatgat
    24541 tcgaaaaaac cgacaattac tgcatgattt atctgcaatc actggggtcg tcatcgaaac
    24601 gcctgctttg aacaaattgt gtaatttagc tgaacagtat gaaggagccg caaaatcttc
    24661 tggtgcaggt gggggcgatt gcggaatcgt aattgttgac cagaaatctg gcattcttcc
    24721 tttaatgagt gcatgggaaa aagcagaaat cactccactg ccgttacatg tctatagcga
    24781 tcaaagaaag gaaaaccgat gaatcgaaaa gacgaacatg tttcactagc taaagccttt
    24841 catgataaac aaaaaaacga atttgacttc gtacggatca tccataaccc tttgccgcaa
    24901 atagcagttt ctgatgtcga tctaagtaca caggcagttg ggtttacact aagcagccca
    24961 ttttatatca atgcgatgac tggcggaagc gaaaaaacaa aaaaaatcaa ccaagatctt
    25021 gcaatcgtcg caagagaagc agatttgatg attgccactg gttcagtcag cgctgccttg
    25081 aaagtccctt ctttagccga tacttatacg attatgcgac aagaatatcc tcacgggaaa
    25141 atcattgcca acatcggtgc agggacttct gtcgaaagag ccaaagaagc tatccgattg
    25201 tttcacgcag atgccttaca gattcatttg aatgcacctc aagagttggt tatgcctgag
    25261 ggagaccgtg attttaccaa ttggaaagta cttattcaag aaactcaaac agccattgac
    25321 gtccccctca tcgtaaaaga agtcggtttt ggcatgacaa gagaaacgct caatgatcta
    25381 gctgctttag gagttcatac agttgatatc agcggtcgaa gcggaacaag cttcacccag
    25441 atcgaaaacg cccgtcgctc caagcgggaa ctgagttatt tagctgactg ggggcaatcg
    25501 acagtctctt cattgcttga agcaaatgaa gcagacactt ctatggaaat cctagcctcc
    25561 ggaggtatcc gcaatgccta tgacatattt aaagcccttt gtctcggcgc aaatgccgtg
    25621 ggtacttcag gaaccgtact gacgcatctg atgaatcacg gcgtagaaga aactattata
    25681 ctgatgaagc aatggcaaga agagcttcgt ctgctttata ctatggtcgg cgcaacgaat
    25741 accgcggccc tacaccaaca atctctgatc ttttccggac cagtgaagga ttggtgcgaa
    25801 gcaagaggga tcgacttagt gaagtatgga agacgcacag aaaagagcgt gggacaaaag
    25861 taaagaacac ttttgtctca cgctctaaac acgtacaaac ggcggaagta aaagcaactc
    25921 cttcggaaat aagctaaaat tcacaaaaat ttgaaaagca attttcgtga attccttctt
    25981 atttcttgga gttaaatgct tctgtcccag cctctttttt cgaataaatg atgtttaatc
    26041 agctgtcctt cggtattcgt tcaatccgct gatttcacaa cctctccact aaacagtgta
    26101 ctaaagctct tttaaggttg ttttccgata taggcaagta tgcctccgtc cacatacaag
    26161 acatggccgt taacgaaatc agatgcgcta gaagccaaga agacagatgg tccagccaaa
    26221 tcgattggct cgccccagcg tgctgctggc gtacgtccaa cgataaattg atcgaatgga
    26281 tgacgttcac cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt agcaatataa
    26341 cctggtccaa ttccattaca ttggatattg tattgaccgt attcagaagc gatgtttttc
    26401 gtcagcattt ttaatccgcc ttttgccgca gcataagcac tgaccgtttc gcgtcctaat
    26461 tcactcatca tggagcagat attgatgatt ttaccgccgc ctttttcgat catatccgga
    26521 ataactgctt ttgaaacgat gaatggggca tttaaatcca cgtcgattac ttggcggaag
    26581 tctgccgcat ccatttcgat catcggtgtc cgtttgatga tccctgcatt gttgactaga
    26641 atatcaactg aacctacatc ttctttgatc tgttgaacca tcgcttgaac cgcttcttca
    26701 tcggtcacat cgcagacata accttttgca tcgatgcctg cttctttata atgcgctata
    26761 ccttcatcaa ctgattcttg attcaagtta ttaaatacga tttttgctcc tgctgtagct
    26821 aaagagcgtg caatctcaaa tccaatacca tagacagcac cggtgactaa tgctactttt
    26881 ccatctaaac gaaacatatc catactaaat tccatcattt tctctccctt atgcttattt
    26941 taattgatcc atttgcacca tatccatgtc tgtataggtg atattttctc cgcacattgc
    27001 ccagatgaat gagtaattgc ttgtacctac acctgaatgg atcgaccagc ttggcgaaat
    27061 aatggcttgt tcattgtcta caactaaatg ttttgtttca tctggtttgc ccatcatgtg
    27121 gaacacacgt gtatcttcat tggcataatc aaagtagaca taagcttcca tacgacgttc
    27181 atgtgtatga caaggcatcg tattccaagc gcttcctggt tctaaaatcg tataacccat
    27241 ttgtaattga cagctttcgc atacatttgg atggatgtat tggtagattt tacgttcatt
    27301 cagtgtcagg ccttcaccag tctccattgg ttttacttgg tcgatactga tttttacatt
    27361 tggatattta tgatgagctg gaacagaact aatatagaat ttagctgggt tttcagcatc
    27421 ttgagaagaa aatacaacat gtcgtgtttc ttttccgata tagtatccgt cttgtttttt
    27481 catttcttct ttttgcccat cgatctcgat gaatcctggt ccaccgatat tgatcacacc
    27541 tagttcacga cgctctaaga agtaatcaac acctagttct ttgtctaatg tgatttctaa
    27601 ttctttttca gtaggtgtca ctccaccaaa gatcattcga tcgttgtgcg tataagtcaa
    27661 actgattttc cctggttcaa ataccttttc tactaaaaat tcttttctca attgttccgt
    27721 tgaataatga cggatatctt ctggactgtg tgtataacgt gtttccattt cttgcatttt
    27781 cttctactcc ttctatcgaa tgatttttcc agagccgcat gctaaaaatt gtgcgacttc
    27841 ttcttgacta aattggttgc agtccccatg gatcgtatgc ttcaaggcag aagctgccgt
    27901 agcaaaatca atgatttctt gcggagacat attggataaa atcccatgga gcacgccacc
    27961 tgcaaaagca tctccgccac ctactcgatc cacgattggg gtgattctat ggacttggga
    28021 ttccacatat tcatggttca tccacaatgt ccccttcaat tcattttcac ttgctgaatg
    28081 aacggtacgc ttggtcgaat aaaacacctg aatattcggg aacttttcct gcattttttg
    28141 ataataataa attagaggtt gttcttctgc ttctggcgct ttctcgatac ctaacaaata
    28201 aagagcatcc atttttccag ctgagcagat atctactaaa ggtaaaagct gatggattac
    28261 ttcgctggct tcttgctgtg tccataattt tccacgatag ttgatatcga agctgatcag
    28321 gcagccagct ttttttgcag cttctatcac ctgtttcgtc attttttgcc attttttaga
    28381 caaagctggg gtgattcctg aaacatgaaa tatatctatt ccttggaata actcctctag
    28441 cgaccattcc aaatgatcca tttcagcaaa acttgatccg gcacgatcat agatcactga
    28501 ggcggcgcgt tcgccactac cagtttccat ataataagta cctagtcttg atccaccaga
    28561 taatacctgt ttcgtatcta ctccataaca ttgcaagtgc tttttgactg cttttcccaa
    28621 tgcgttatca ggtacttttg tagcaaaaat cgcttcatgt ccgtaattag ctaaagaaat
    28681 tgctacgttt gcttcccctc cgccataatg ggcagcgaat cgttcacttg aatcgatacg
    28741 ggttccctgc tctgtggaca atcgcaacat gatttctccg agtgtcacta cttttcccat
    28801 aagcagctac ctcttgattt ctttcatttt tgctgcatac tgttgcgcga cttccgttac
    28861 tttgtcaaaa tctcctgtag ctgcgggtgc taaaaggttc ccacctactc cgactgttac
    28921 gacaccagca tcgaaccact ctttcatatt gtccagactc acaccgccag ttggcatgat
    28981 attgacttga ggcatcggcg ccttgaaggc agaaatgata cttggtccat aagcgcttcc
    29041 aggaaatagt ttgacgatat ctaccccgct tttcagcgct gttttgatct cggtaatagt
    29101 catacatcct ggtaagtaag gaatttgata cagattgcac atctctgccg tttcttgatc
    29161 aaaggaaggg ctaacaatat actctgcgcc agctaagata gccaagcgag ctgtgacagc
    29221 gtctaatact gtgcctgcgc caatcactat ttctggttgg tctttgtaga atgacaaaag
    29281 ttctttgatt acttgatctg cttgagggac tgtaaaagtc aactcaatac cagttagacc
    29341 acctttgatg attgcgtggc aagcattcaa agcttcttct ttgctttttc cgcgtaccac
    29401 cgcaatcact ccggcgtttt tcaatcgaga taaaatatca atttttttca tcttctccac
    29461 ctatttccta tttagaaatt ttattttata acttggaaat ctatacccat attatacggc
    29521 agaaaagaaa aattgcaaac gcttttttca ttatttttga attctttgct cgatttcgct
    29581 ttttgtttgc ttgagggcac tgattatttg ttcttttgtc tcatctgtta aacgatattt
    29641 cggcatactt acactaaatg caccaatgat ttgttcgttt ttcacgattg aggctcctat
    29701 acaaaaaata tcacgttcga tttcttcatc gtcaaatgcg atgtgggttt cttttaccgt
    29761 tttgatttcc tttttcaacc gtaatgcatt ggtgatcgta tattctgtgt aagggatcaa
    29821 cggatgcttt tcaagataat cagtgtactg ctcttcactg aattctgcta atacggcttt
    29881 gcccatcgca gaattgtata atggtcgggt gattccgatt tttgaagaca tgcggatcgt
    29941 ttgatgtttt ggatctagtt tgttcacgta caagatctca ttgttgtcca atacccctaa
    30001 gtggatagtc tcatcgatct tttgctgcag ctgttctaaa aacggtaaag tcaactctac
    30061 caaatcgatt tgttcaatgt ttttattcgc gtagcgaata agctttgcac ctaaacgata
    30121 gtttttctct gaatttttat ttacgtagcc gatcatcaac aaagtatcta gtattttcaa
    30181 gactgtagaa gaggtcattc ccacaccctt tgcgatcgtc tgcaaagaga catcttggtt
    30241 ttccgctaaa tagtctagta ttttagaggc tttgatcaat acagttccat aaggcttttt
    30301 attttccata taatattctc ctatttcttt ataagaaaaa ttatagcgga aataccatca
    30361 atttaaaaga gatatccaga tataaactac catttctctt tacaggcaat ttaatgttga
    30421 agatccgctt cgtttttaca tcaaactttg atataaagca atgatttctt ctactgtcgt
    30481 ttctcttgga tttcctggtg tgcatacatc tcgcaaagca tcttctgcaa ttgctggaat
    30541 atctgcttct tttacaccta attcagaaat ggtttctggg attccaaccg ctttgctcaa
    30601 ccgatcgatc tcaccacaag ccgcttctct tacatcttct aaagacattt cagctgctcc
    30661 tttaatccct agaacaagag cgatctcacg gtacttttct ccagtatatt ctttgttata
    30721 tttcatgaca gttggcaaaa gtgctgcaca cgctacgcca tgaggaatgt tgtaccatgc
    30781 acttaacggg tgagccatcc cgtgtaccag tcctaaaccg acatttgaga agcccattcc
    30841 cgcaatatac tgtcctaatg ccattttttc tcgcccattt tgatcgccat ctacagaagc
    30901 ttccaaagaa tggccgatga tttcgattgc ttttaaatgc agcatatcag tcatttccca
    30961 agcgcctttt gtgataaacc cttcaatagc atgcgtcata gcatccatac ctgttgatgc
    31021 agccaatttt ttcggcattc ccatcatcat atcgctgtcg atgaacgcga cgattgggat
    31081 atcatgagga tcaacacaaa caaattttcg gtgatttact tcatctgtga tgacgtagtt
    31141 gatcgttact tctgctgctg ttccagaagt cgttggtata gctaatatag gtaaacatgg
    31201 atttttcgta tcagcaacac cttctaaact aatgacgtct gaaaactctg gattcgtcaa
    31261 aataatccca atggcttttg ccgtatcgat tggcgaacct ccaccgattg cgatcaaaca
    31321 gtctgcctct gcctctttaa caaaagccac tccatctttt acattttgga tcgttggatt
    31381 aggtacgatc ccgtcgtata aagcataagc gattgctgct ttatctagaa gttctgttac
    31441 ttttgttgct ataccgtgct cgatcaatcc tttgtcagta atgacagctg ctttgccgaa
    31501 tcctcgctgc tgaaattcag ggacgatcga ctcaatcgct ccttttccaa aatacgatgt
    31561 ttcatttaaa atcattcgat tactcatttt ctattcctcc taatttgtat tatttttcac
    31621 aataaaaacg aaagaacccc aagcaaccgg ttgcccagtt gcttgttgct acagttcaaa
    31681 gactggcatc aattcccaac tgactggaga ggcatctgga tttgtctcca tgatatcttc
    31741 catgtacttc caccattttt gattgataga tgtatttgcc attttttccc ataacttttc
    31801 atcttcgatc actaagtaac caaataacaa acttgtactc ggatcaagat agatcgaata
    31861 gtcgatgacc ccgtgttcac gaagtgctgt acgcatttct ggccaaagtt catcatgtct
    31921 ttttttgtat tcttcttgtt tttctgggta aactttcatg caaaaagctt ttttcaccat
    31981 gtcagacacc tgctttcgta tgaacttcct ccaaataacc tgcttttgga gtgacaccaa
    32041 acgcatctgc taaatgccac aggttttcgt ccgtgattgt ttgcaagatt ggtccttgcg
    32101 ctttgacgta cgtatagact tctgctgcct tttctgctgt ctcgatcaaa ccaaagactt
    32161 catccatgtc ttttcctgac ccgtagattc catgttgtgg ccataaaacc aatcgagttt
    32221 ctttcatttt ttcagctgtt gcttccccaa tctcatttgt tcccggaacc atccacggaa
    32281 tgatggaaat tccttctgga aaaacaacta agcattccgt acacatttgc catagcgtac
    32341 gactaaagga acgttcgtct aattcatgcg taaaactcat cgctaataga tgactagcat
    32401 ggcaatgcat aacgatccga ttatctgggt ctacttctaa acgggcgata tgactcatga
    32461 aatgacttgg caactcgctt gtaggaaggc attcgttttc cagcccccac aaaagttcca
    32521 gtgttttgcc atcttcagca actcgaacga ttccaagatt ctcggcaggt tcgctcgcta
    32581 catttttaaa atatcttcct gaaccagtta caataaaata gcatcctgct aaacggctgg
    32641 catcaaaaat catcgagatg cggcggatca ctttttcagg atccaagaac ggtgtgatct
    32701 cttcttcttt tagtaaataa gaaatattcc cgccatttct ttcatcccat cccaagcgat
    32761 aaagattcga cgtcgtttcg atcatttctt tcacataagg cgcttgtaaa atatctattt
    32821 ttttcaatat agccacactc ctattttctt aattccaaca cgtctttttg atattcctgg
    32881 acttcttcca gccaatccaa accaactgga acttgatttt tgtaacagaa ataattccat
    32941 acatcggcat acgggaaatc tttcaattct tccgtataag ccattcttga agtaaagtca
    33001 aaagccagct ccatttcttt caatcgctca actggttcca gcatagcttt cagcagtgct
    33061 ttttgtgtat ttctcgtacc gatgacccaa gcagcgatac ggttgatcgt tgcatcaaag
    33121 aaatccagac cgatatttgt tttttccaac aattcgttac ggactaattc tttagcgatt
    33181 tcctgcaatt catcatccat caaaaccaca tggtctgaat cccaacgcac tggacgggaa
    33241 acatgcaaca tgattccttt gctgaataaa gctaatgaag ataatttatt ggaaatcact
    33301 tctgttggat gaaaatgtcc agcatctaga caaacaagtt tgtcacgagt caacccgtaa
    33361 cccatataaa attcatggga accgactgta taagcttcag cgcctaaacc aaatagtttg
    33421 ctttctactg catcttgtac ataccgttca tctaactctt ctgtaaaaat ttcatcgagt
    33481 gattccatta aacgttcccg tggggtaaaa cgatcaatcg gattatcttt catcccatca
    33541 ggtacccaaa agttattgat acttgtttgc ccagtttctt tcccaaagta ctccgcgatt
    33601 ttgcggcttc ttttcccgtg ttcgatccag tattcacgaa cttctttatc tggatgagcc
    33661 aatgtaaagc cgtctttcat cattggatgc gaaaagaaag tcggattgaa gtcaagaccg
    33721 atttcttctt tttttgccca ctctacccat ttttcaaaat gtttcggctc aatttcattt
    33781 agatcgacag attcttctgt atccaaatag atagcatgaa ggttcaattt gtgtttgccc
    33841 ggtatcaacg aataagcctt ctccagatct tgtctaagct cttcaggtgt tcgtgcggct
    33901 cctggaaaat tccctgtcgc catgattcca ccggtcaatt cgccatccgg tgtcaagaaa
    33961 cctttgacat catctccttg ccagcaatgc atagaaattt tgatgtcctg tatcttttct
    34021 agtacagcat ctgtatctac accgatagtt gcatattttt cttttgcttg ttcgtaattt
    34081 ctttcaatcg tgttcatacg attactcctt tgattttatt cacgtaccat tccatcgtgt
    34141 tttgcttatt ttcatctggg taaaaagttt cacatggaaa cgactgtttg attacatttc
    34201 ttgcttgttc tatattcgaa aactgacctg tactgagcat ttgcatggca acattcccga
    34261 ttgctgtggc ttcactaggt cctgcttcta caatgattcc ggcataatct gctgtcaatt
    34321 gattcagtag acgattattc gatccgcccc ctacgatgtg cagcttttcc aatttcgttc
    34381 cagttaagtg ttctactttg atcagctctt gtgcgtaaca aagagcaaga ttatcataca
    34441 cacaacgagc gatagctccc gatgagacag ggactggctg ctctgtttca aaacagtatt
    34501 gctgaatctc ttctatcata tttgggggat tcaaaaatcg agggtcattg atttccatca
    34561 cttgctgcag cggtttttct tttgcagcaa gctctgccaa ttccgcataa ctgtactcgt
    34621 actcttgcat tcgggcaact tcctggatca accacatccc catgatgttt ttcaaaaaac
    34681 gcaccgtgtt atgggcaccc cattcattcg tataattttc agaaaaagca ttgcgagaaa
    34741 cattggcgat ctttgtttca atccctaaaa gcgaccatgt cccactgctg atataacccc
    34801 aacgttctcc ttgacctggc gttcctaata cagctgacgc cgtatcatga cttgctactg
    34861 tgataaaggt cacatctggc aaatcatatt ttgaaaattg ttctttttgc agatctccta
    34921 gtatcgttcc cggctctgtc aatggtggaa ataattcctt cgagacaccg attttttcaa
    34981 gaagttctat ttcccattct tttgtccctg cattcagcag ctgcatagta gatgcattag
    35041 ttttctccgt caccatgtta ccagtaaaga cataccctaa ataatcaggt atcagtaaca
    35101 atttatgtgc ttttgccaaa ttttcttttt cttcagcaaa caattggaac aaggtattga
    35161 aaggctggat ctgaatccct gtctgctcat acaacttatc tagagaatag atttcagaaa
    35221 actgttctac tgctccattg gtacgcttgt cccgataagc aatcggatca tttatccgat
    35281 ccccttgttc atcaatcaga cagtaatcga ctgcccatgt atcgatcccc gcaaaaaggc
    35341 gatcaatccc tgtctgcttt aatttttcaa atccaatcaa aatctctttg ataagataat
    35401 cgatcttcca gcgatcgtga ccgtctttgt tccgaaagcc gtttttgaac cggtgtactt
    35461 cttccaagat caaagaacct tttgcatccc tcgtactttt cattagtcgt ccgcttgatg
    35521 caccgatatc gatggcaaca aatgttggat tcatttctcc acctctttta ctttattgtt
    35581 gattagcact ttattaataa agcgttatca tttattgaca atgctatcat aatacagaat
    35641 cttatatttt gcttcacact attttggtat ataattgcat tatcttgaca agttgggagt
    35701 gtttacatgg attactggga aaaagccaac cactcatggt cggaagactc aaaaagagtg
    35761 atcttgacgc ctacacaaag aagcaggaat ttattttttt atatccaaga aatcggttac
    35821 ttcaaagcac caatccctta ctatactgaa cgagcacatt taccttctta cttgataaag
    35881 tttactttgt ctggcaaagg ttcgcttaca tataatgata atcagcaaca gctaaaagct
    35941 ggagacttat tttttattga ctgccaagaa tatcagcgtt atcaaacgat cagcgaagaa
    36001 ccatgggaaa tggattggat ccatttgaat ggtcagaacg tccaatcatt ttatcaggag
    36061 ttcacaaaag acggccataa caccttccat tcaacaggag atccatcaga aaacaagatc
    36121 caccatttga tcacagaatt gatccacatg caacaaggca cgaatgcccg taccgatttc
    36181 cagtcctctg tagctatcca cgagttgctg aatgaactga ttttgcaaaa gtatcagcta
    36241 gatttcgaag aagaggatat tcccgcctac gtttacaaac tgaagcaata cttagacgaa
    36301 catttcaaag aaacgattac acttgatcaa ttagaacaac aattcttatt gaataaatat
    36361 cagctgagca aagatttttc caaatttatc ggttctccac caatcgatta tttgatcagc
    36421 aaaaaacttt cttacgctaa agaccttctt cggtatacag aatttacggt tcgaatgatt
    36481 tccgaagaaa tagggattga gaattttgct tactttagcc gcctgttcaa agcaaaaaca
    36541 ggactatcgc cgagagactt tcgtaaattc agctgattca tataatatgt acgtcatttt
    36601 ttcaaaatat tttgaaatat atagataaaa ataaaataac cgcagtttct gcttctatgt
    36661 aaacaacagc agctactgcg gttatttttt agtgatcatt ctcattgttc atcttcaaaa
    36721 tgttcataat gaaccaactg tttctgtgta tcacgtttca aaaattcttt aggcgttatg
    36781 ccgaactctc tcttgaatgc tttcagaaaa tgcggataag aagaaaaacc acttttacta
    36841 tatacagcac tagagctgcg gtattctcgc aacaagtgct tggcatagat cagctttttc
    36901 ttcattacat actgatgaaa cgtaaatcca gtgtactttt taaattctcg tgtgatatgg
    36961 tatttgctga caaaaaactg cttttccatt tcctctaaag aaagcggatg agataaattt
    37021 tttgagatat attgtaacat ttgttccaca cgctcattag gaactacagc attctgttct
    37081 tgcgcactct cttctttttg tacaacttga ttgagaaaaa tcaagtattc taccagagct
    37141 tgttcatatt ttatgtcttc accgtaaatt tttttatcag gaggatggtc gataaatgat
    37201 aatttttgct tcaatacttc ctgttctatt tgcaatacac gactcctctt ctctcccatt
    37261 ggttgaaaac aagccgatag attagaccat ggcgtggaac gactttctag aaaagaaggg
    37321 gtgacaaaga tatagacacg ctcgaataca tctgaagatt ggcggacgat acgatgcaaa
    37381 tcattggaat ggatgagcag cacacttcct gctttgatcg taaattgtcg tccgttcaaa
    37441 taaaaagtcg cttctccctt taatgtagca tggatctcat agaaatcatg atgatgatag
    37501 atcgttttat tttggctgac gacatctttg acatgataga tttcatactg atcagaaacc
    37561 atcgtattga cttgttcacc gctgattttc ttttggagca tgtgccgcac ctccttttgt
    37621 tcacttatgc acaattttat tataccacgt tttttgtaat caaaaagagc tgcttttata
    37681 tactcttata aaaaagaaga gacgtggcat cttctagccc cgccccctcc attctaaata
    37741 ttagttaacc aacgtcttct ttacattatt caagaagact tcttccataa cgaatcccat
    37801 catcttttgt ttcttcaaga accacataca ggtacggacg attcttcatg atgatttctt
    37861 tgatttttgt gtactcgatc acaccgtctc ccaaatttac cggcacgatt ttttcctgtt
    37921 cgacacgaaa atctttcaaa tgaaccgcac atatcttttc accaaaacgt tcaaaagctt
    37981 cttctaccaa ttggacttgt tcttgataat cactacttgt gatcaaattg gtcggatcta
    38041 aaatgattcc tagatagtcc gaatctactg cttggatcag ctgctctacc cttgctaaag
    38101 aatacaccgg atgattcaaa cctggttcta tccccaccat catttggtat ttttctccag
    38161 cttttaccaa gcgacggata acggacacag cttcagcaaa tgcttcatct gtgaaatttt
    38221 cttccgtata ttggatttcc ggcaaaacac atccagtttc cgaagcaacc atcgacgcac
    38281 caaaggaaga agcatagcga agatactgct cgaactttgt cagcaactct tgtcgtatcg
    38341 ttaaatctgg atgaatcata ttgatatagc aacttaaaat tcctacggtc acttcttgct
    38401 tttctaaaac tcgtttgaca taatttccca tcccagaatt gatctcattt gcgccagaag
    38461 gtatttcagg aaacgaaaga cctaaggcga gctgcagtgt atgaatccct tgttcttttg
    38521 ttttttctgc aagatcttct atcgtttgga cttcggaaag atcatgtcct ctaattgtaa
    38581 tgttcaacat aactattctc ctaactcagc atattgggta gccacatgac gatttgcggg
    38641 aagtaagtaa tcaaaaacaa gacgactaag ataaccccat aaaaaggaag aagcggtttc
    38701 agcattgcct ctgcttttac gcctcctaca cttgcaccga catatagtcc tgtacctact
    38761 ggcggcgtga tggagcctac acacagattc atgattgaaa acaacccgta atgtacagga
    38821 tcaaccccta cacttgtgac gattggtaaa aatattggtg taaaaatcaa gatagcaggc
    38881 gccacatcca taaacatacc caccagtaat aacacaagat tgactaataa cagaataata
    38941 aatttattat ccgttacgcc cataatcaac gaactaattg cctgaggaat accggtaaac
    39001 gccatggcga aagacatcat ggaagaagta gccaataaga acatgatcgt ccctgacatt
    39061 gctactgatt gaaacaacat attaggtata tcttttagtt ttaccgtttt atagtaaaac
    39121 atagcaatca acagggagta aatgacagct atcgctgaag cttctactgc tgtaaatatc
    39181 cccgtcaaga taccaccgat aatgatgaca atcaatagaa ggctaggaat cgcctctttt
    39241 attatttttc cagcatgagc catctgtcct ttttcgacaa cagggtattt attcttttta
    39301 gcaacgatga atgctactag catgattcct agcgcccaca aagcgcctac gagataacct
    39361 cccataaaca aagcagagat cgaagcacca ccagcaacaa gtgaatacat gataaaagca
    39421 gtacttggag gaatgaccat acctgtcgga gctgacgcga tattgaccgc tgtcgcaaac
    39481 ttccggtcat atccttcttc cacttgctga gggatcaaca ctccgccaat cgcggtagaa
    39541 gcggcaatgg ccgagctaga gatggaacca aataaagcat tccctaacac attcgtatga
    39601 gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa aatccactag tttcttagct
    39661 atccctccat tattcatgat aatgcccgag agaacgaaaa acggaatagc gatcagcgaa
    39721 aaactattga tacttcccac cattttttga gctgaactaa aagcagaaac atcaaacggt
    39781 ataactaaca ataaagtcat catcgatgag aatgcaatac ttatagcaat cggcatccct
    39841 aatacaagca acacaacaaa tacaaaaaat aacactaatc cggcttctat tgccatttta
    39901 ttcctccatt tttatatttt ttcttgaatt gctggcttgt tgcttaatgt atcgattaga
    39961 ttgatcaggc tatagataat gatgagtacc cccgatacag gtactgacag ataaacatat
    40021 cccataggaa tacttaaaga aggcgagatc tgccccattg tcaatgtgac agcattccat
    40081 ccgccataca ccatgatcat cgcagcaaat accagaaata aactttgaac aacaacttct
    40141 aaaattttac tttttttgcc agttaatttt tcacttaaaa ccgttacggc taaatgtgct
    40201 ttttgtccca caacatacgc ggaagacaac atggcaaacc agacaagacc gaatcgaaca
    40261 agctcttccg tgaccgtatt tggatttcct aaaaccgttc tggaaaatac ttgccataaa
    40321 acaacagcga ccatcccaac aagcaaaaca gctcccagga tctctaatac cttgtttaaa
    40381 atcattttca tcacagctac gctccttgct cttggatctt ttgataaagg tctttgtagg
    40441 taggatcgtt tttgaatata tcatgaagtg gggcaactgc ctctcgaaac ggtgtattat
    40501 ctacatcatt gaattggact ccatgttctt cttgtgctaa agctttttct tcttcaattg
    40561 cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt cgctgcttct tcaatggctt
    40621 tttcctgctc atcagttagc tgttctttga tggcatcatt catgatgatg atatctggca
    40681 cacgtgtatg ctcatcatag gaatagtatt tagccacacc accatgtcca gctgtataca
    40741 agacaaattc attgttttct gatccgttga tcaaattgga ttgaagagac gtgtacactt
    40801 catcacttcc cataggtacg ggcgaaccgc ctaaaagctc taccattttg atagcggttt
    40861 cgctttgcat cacacggatc ttctttccct tcaaatcttc cggcgtatgg atcggaccgt
    40921 ctaccatata aaagctgcgc tgaccagaat cataataggt caatccggta aatcctaaat
    40981 ttttggtcga ttggtagatt ggctccatta tttcttcatt atccatcacc ttaaagaaat
    41041 gttcttcatc attgaataga taaggaactg aaaaaatcga ataatcttta gagaaacttt
    41101 ccaacgctga ggcacttact tttgtgaaat ctactgctcc tgactgtgtc aattcgatca
    41161 gttcacgttc tccacccagt tgtccatctg ggaaatactg gatctgtacg gaaccatctg
    41221 ttttttcttt tactaaacgc ccaaattcat tcattgcatc aattactggc tggctgttgc
    41281 ttgcataagc aaagcgcaag gtaactatct catttacatc tgcatctgcg acagttgcca
    41341 cactagaatt atttccgcag ccgcttagac ctagcagacc taggctaagc gaacttgctg
    41401 caaacagcag tgatgttttt ttcatatggg tttcctttct tttaacaata agagacgagc
    41461 aattctgtca aacaaagcac cgttaacgac tgtccataag gcattgctgt gattccgata
    41521 ttccgataaa aatctaaact gtcccccatt cctgtcccga ttgaaacgtt ttgaacttct
    41581 cctttttcat cgatttgttc aagcaatcct tggatagcac gataagcgac ttctttatat
    41641 tcttgtggta aataacgttt atggaccgct ttcaaaatac cgtaagcaaa tcctgcagtt
    41701 gcagaagatt ctacgtaaga agaaggatca tccaacaacg tatgccataa accgctttca
    41761 gactgataag tttttaatgc acgcacttgt gcttcaagtg tttcgatcaa taatttgcgc
    41821 aatgcatctt ctttcgataa ttctaagatt tcaatgatct ctggaatagc aatcgtcaac
    41881 cagcagtttc cacgagccca aaatgcttcg gcatagttat gatttccttc aaacgtccag
    41941 ccgtgatacc acaatcctgt tcgtttatcc atcaagtatt tgatatggat cataaactgg
    42001 taacgagctt cttctaaata ttctgggcga ttcaacagtt tcccgatttt tgccaaagga
    42061 agcactgtca tcatcaatgt atcgtcccac aactgattct tattttccgg tccatatgtg
    42121 gcatgctgta acccgtcttc atttgtccga ggcatgtcat tcatgaccca ttctgcccat
    42181 tgttctaaat aaggcaaata gcgtgaatct ttcgtgtctt catacaagta agccatagtc
    42241 aatagcggtg ccattgtatt gacgtttttg ggcggagcgc cttcttccat acgatcctca
    42301 aaccattcat tgaccacttg ataagcgcga ggattttttg tcatctggta atttttataa
    42361 ataccatata gaccgacacc ttgtggccaa ttccacactg tccagctttt gtcatctact
    42421 ttcagtccat caaagtccaa caaaaattca ccattatcgt ctttgatcgt tgtcaggttt
    42481 tcaacgagca ggtcaatttt ttctttcact aattctttag gtacaaaaat ctcttcttta
    42541 ataatcatct ttacgccacc tttttaaacg ctttcatttt atatccttaa tatagcagta
    42601 ataccgtcca ctataaatat cgctttttga acaaaaactg tctaaaattg acctaaatgt
    42661 taaataacaa aagtaacttt cagttacttg gaaattaaaa aaagaacttg gagaaacaac
    42721 gttttccaag ttctttttca tctattttct tatctttttt ctacgctttc ttttgcttat
    42781 ccgttgtaag catcaatgat gatctgtttc aattcagaga tcaatggttc tttcggatta
    42841 gctgtcgtac attggtcttc gtaagctaat tcagccatac ggtctacggt tgtatctagt
    42901 gtttcttgtg tgactccttg agcttttaga ctcatgttga tgccgacaga ttttcctaat
    42961 tcataaacag ccgttgctaa agcttcgact aattcctctg ttgtttcgcc tttcagacct
    43021 aagaattttg cgatatctgc gtaatctgta tctgcacgga aatagtcata tttagggaac
    43081 atcgcatgtt tttgtggatc ttttgcatta tagcggataa tatgcggtaa aagaatcgca
    43141 tttgtccgtc catgagggat cccgtattcc ccgccgattt tatgtgcgac tgagtgacaa
    43201 atacctagga atgcgttagc aaacgccata ccagccattg tagaagcgtt gtgcattttt
    43261 tcacgtgatt ccatatcagg tgtttttact gatttttcta aatagtcaaa gactaacttg
    43321 attgcttgta ggctcaatcc acgtgtataa tctgaagcca tgactgaaac ataggattcg
    43381 attgcatgtg ttaacacatc catcccggta tctgctgtga cagatgctgg aactgacatg
    43441 acgaattgag gatcgacgat tgcgacatct ggcgtcaatg cataatctgc taacggatat
    43501 ttcacatgtg tttcactgtc agtgatgacc gcaaatggcg ttacttcaga accagtccct
    43561 gatgttgtcg ggatacatac gaattgtgtt ttttctggtt tgtcgatctt atatgtccgt
    43621 ttacggatat ccaagaattt ttgttttgca ccaaagaaag atgtgtctgg gtgttcatag
    43681 aacatccaca ttcccttagc tgcgtccatt gcagaaccgc ctccaagagc gattactgta
    43741 tctggcttga agtctgccat cagtttcgtt cctgcgtata cggtatttgt cgatgggttc
    43801 ggttcaacat ctgagaagac ttcgatcttc acatcatttt tacgtcgtgc caatacttca
    43861 cgaactgtat cgcagtaacc gaattggacc ataccagggt cacaaacgat catgacacgt
    43921 tcaacgtttt ccattttctc taagtacaat agagagttct tttcaaagaa gatttttggc
    43981 ggtaatttga accattgcat gttatttctc cgtttcgcta cagttttgac attgattaag
    44041 tttacagcag agacgttacg agaaacagag ttcttaccat aagaaccgca tcccaaagtc
    44101 aatgacggaa tcatttcgtt atagatatca ccgatgccgc cttctgcaga tggtgtattc
    44161 actaaaatac gacaagcttt cattcgaaga ccgaatttga catgcaactc ttcgttttct
    44221 gaatggataa ctgctgtatg tcctaatcct ccaagattca acataccttc acacaaatca
    44281 aacgcatggt ccgtgttgtt agctttgatc atggcaagaa ctggagaaag tttttctctt
    44341 gatagcggat aatctgggcc tacgccttcc aattcagcaa tcagcatttt cgttcctttt
    44401 ggtacactga ttcctgccaa ttttgcgatt tccatcgcag aatgtccaac gattgatgga
    44461 ttgaccgcat attttccttc gttcatcact gcatcttcta atttttgcaa ttcatctggt
    44521 tttacgatat aaacttggtg tgcttggaat tctgctttta ctgctgcata gatttcttta
    44581 tccacgatca cagcttgttc agaagcacaa atcattccat tatcaaatgt tttcgaaacg
    44641 atcaaatcgt tcactgcacg tttaattttt gctgtttttt caatataagc tggtacgtta
    44701 cctggaccta cacctaaagc tggttttcct gtagagtaag cagacttgac cattccagct
    44761 ccacctgtcg ctaagacgat cgcgatacct ggatggttca ttagcataga agtcgcttcg
    44821 atcgaaggat gttcgatcca ttggatacaa ttttcaggag ctccggcagc gatagcagca
    44881 tcacgtacca ctcgtgctgc ttctgcagaa gatttttggg cacttggatg gaaagcaaaa
    44941 acgattggat ttcttgtttt caacgcgatc attgatttga aaatcgttgt ggaagtcgga
    45001 ttcgttgttg gagttactcc gcaaaccaca ccgactggtt cagcaatttc aatcaaccct
    45061 ttttggacat cttcattgat cacaccaact gttttatcat gtttgatatt atgccaaata
    45121 tattcggacg catacatatt tttgattgct ttatcttcgt aaacaccgcg gcctgtttcc
    45181 tctacagcca tcttcgctaa tggcatatgt tgatccaaag ctgccattgc catttcatgc
    45241 acgatatggt ctactttttc ttgatcaaag ctttccattt ctttcaatgc tgcatttgct
    45301 ttttctgcca aatcatcaat cattgcttgt acgtcaattg tcttcgtttt ttctttttct
    45361 attactttag ccatcattat cctcctaatg ggtcatggtt atttgttaaa taattcacat
    45421 gtatatcata caatatttat ttttctatgt aaacactttt ttgtgatttt tttcacagtt
    45481 attttt
//
LOCUS       DAZKPO010000012        44831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000012 DAZKPO010000000
VERSION     DAZKPO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.012"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8NZ_000660"
     CDS             212..604
                     /locus_tag="ACG8NZ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0538359.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8NZ_000661"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8NZ_000661"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0538360.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8NZ_000662"
     CDS             2698..2943
                     /locus_tag="ACG8NZ_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538361.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8NZ_000663"
     CDS             3165..4775
                     /locus_tag="ACG8NZ_000663"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0538362.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8NZ_000664"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8NZ_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538363.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8NZ_000665"
     CDS             5067..5936
                     /locus_tag="ACG8NZ_000665"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0538364.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8NZ_000666"
     CDS             6101..7372
                     /locus_tag="ACG8NZ_000666"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0538365.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8NZ_000667"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8NZ_000667"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0538366.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8NZ_000668"
     CDS             8763..9026
                     /locus_tag="ACG8NZ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0538367.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8NZ_000669"
     CDS             9471..9920
                     /locus_tag="ACG8NZ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0538368.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8NZ_000670"
     CDS             9977..11395
                     /locus_tag="ACG8NZ_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0538369.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8NZ_000671"
     CDS             11395..12255
                     /locus_tag="ACG8NZ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0538370.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8NZ_000672"
     CDS             12429..13202
                     /locus_tag="ACG8NZ_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0538371.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8NZ_000673"
     CDS             13349..13714
                     /locus_tag="ACG8NZ_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0538372.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8NZ_000674"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8NZ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0538373.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8NZ_000675"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8NZ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538374.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8NZ_000676"
     CDS             15560..16216
                     /locus_tag="ACG8NZ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0538375.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8NZ_000677"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8NZ_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0538376.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8NZ_000678"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8NZ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0538377.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8NZ_000679"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8NZ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0538378.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8NZ_000680"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8NZ_000680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0538379.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8NZ_000681"
     CDS             21888..22202
                     /locus_tag="ACG8NZ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0538380.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8NZ_000682"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8NZ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0538381.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8NZ_000683"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8NZ_000683"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0538382.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8NZ_000684"
     CDS             23546..23875
                     /locus_tag="ACG8NZ_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0538383.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8NZ_000685"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8NZ_000685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0538384.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8NZ_000686"
     CDS             24863..25687
                     /locus_tag="ACG8NZ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0538385.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8NZ_000687"
     CDS             25680..26027
                     /locus_tag="ACG8NZ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0538386.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8NZ_000688"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8NZ_000688"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0538387.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8NZ_000689"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8NZ_000689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0538388.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8NZ_000690"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8NZ_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0538389.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8NZ_000691"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8NZ_000691"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0538390.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8NZ_000692"
     CDS             31684..32451
                     /locus_tag="ACG8NZ_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538391.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8NZ_000693"
     CDS             32441..34483
                     /locus_tag="ACG8NZ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538392.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8NZ_000694"
     CDS             34764..34913
                     /locus_tag="ACG8NZ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0538393.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8NZ_000695"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8NZ_000695"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0538394.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8NZ_000696"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8NZ_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0538395.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8NZ_000697"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8NZ_000697"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0538396.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8NZ_000698"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8NZ_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0538397.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8NZ_000699"
     CDS             39330..40412
                     /locus_tag="ACG8NZ_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0538398.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8NZ_000700"
     CDS             40412..41215
                     /locus_tag="ACG8NZ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0538399.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8NZ_000701"
     CDS             41285..42025
                     /locus_tag="ACG8NZ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0538400.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8NZ_000702"
     CDS             42036..43040
                     /locus_tag="ACG8NZ_000702"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0538401.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44830)
                     /locus_tag="ACG8NZ_000703"
     CDS             complement(43085..44830)
                     /locus_tag="ACG8NZ_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538402.1"
                     /translation="MAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTI
                     PTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSL
                     AVIDLPANYIGKKLTLTVSSPYQNYAGLPPKFYLGTTGPLISFIYSQSVPQVLTMIIA
                     VFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFL
                     SHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPI
                     ANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTIN
                     WSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNT
                     LRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQK
                     LAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHE
                     QEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFI
                     DRITVQLSRNYPNPV"
BASE COUNT        14407 a         7823 c         9339 g        13262 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa atttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat t
//
LOCUS       DAZKPO010000013        44157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000013 DAZKPO010000000
VERSION     DAZKPO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.013"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..485
                     /gene="rpsP"
                     /locus_tag="ACG8NZ_000704"
     CDS             210..485
                     /gene="rpsP"
                     /locus_tag="ACG8NZ_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0538403.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            497..748
                     /locus_tag="ACG8NZ_000705"
     CDS             497..748
                     /locus_tag="ACG8NZ_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0538404.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            873..1664
                     /locus_tag="ACG8NZ_000706"
     CDS             873..1664
                     /locus_tag="ACG8NZ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538405.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1834..2355
                     /gene="rimM"
                     /locus_tag="ACG8NZ_000707"
     CDS             1834..2355
                     /gene="rimM"
                     /locus_tag="ACG8NZ_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0538406.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2345..3106
                     /gene="trmD"
                     /locus_tag="ACG8NZ_000708"
     CDS             2345..3106
                     /gene="trmD"
                     /locus_tag="ACG8NZ_000708"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0538407.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3242..3589
                     /gene="rplS"
                     /locus_tag="ACG8NZ_000709"
     CDS             3242..3589
                     /gene="rplS"
                     /locus_tag="ACG8NZ_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0538408.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3721..4857)
                     /locus_tag="ACG8NZ_000710"
     CDS             complement(3721..4857)
                     /locus_tag="ACG8NZ_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0538409.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(4973..5539)
                     /locus_tag="ACG8NZ_000711"
     CDS             complement(4973..5539)
                     /locus_tag="ACG8NZ_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0538410.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(5675..6328)
                     /locus_tag="ACG8NZ_000712"
     CDS             complement(5675..6328)
                     /locus_tag="ACG8NZ_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538411.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(6378..6806)
                     /locus_tag="ACG8NZ_000713"
     CDS             complement(6378..6806)
                     /locus_tag="ACG8NZ_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0538412.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(6811..7185)
                     /locus_tag="ACG8NZ_000714"
     CDS             complement(6811..7185)
                     /locus_tag="ACG8NZ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0538413.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            7489..7674
                     /locus_tag="ACG8NZ_000715"
     CDS             7489..7674
                     /locus_tag="ACG8NZ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538414.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            7687..7935
                     /locus_tag="ACG8NZ_000716"
     CDS             7687..7935
                     /locus_tag="ACG8NZ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538415.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            7966..8136
                     /locus_tag="ACG8NZ_000717"
     CDS             7966..8136
                     /locus_tag="ACG8NZ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538416.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(8238..8705)
                     /locus_tag="ACG8NZ_000718"
     CDS             complement(8238..8705)
                     /locus_tag="ACG8NZ_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538417.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            8864..9181
                     /locus_tag="ACG8NZ_000719"
     CDS             8864..9181
                     /locus_tag="ACG8NZ_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538418.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            9114..9593
                     /locus_tag="ACG8NZ_000720"
     CDS             9114..9593
                     /locus_tag="ACG8NZ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0538419.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            9610..9786
                     /locus_tag="ACG8NZ_000721"
     CDS             9610..9786
                     /locus_tag="ACG8NZ_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538420.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            10043..10474
                     /locus_tag="ACG8NZ_000722"
     CDS             10043..10474
                     /locus_tag="ACG8NZ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538421.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            10480..10797
                     /locus_tag="ACG8NZ_000723"
     CDS             10480..10797
                     /locus_tag="ACG8NZ_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0538422.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            10916..11602
                     /locus_tag="ACG8NZ_000724"
     CDS             10916..11602
                     /locus_tag="ACG8NZ_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0538423.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            11577..12926
                     /locus_tag="ACG8NZ_000725"
     CDS             11577..12926
                     /locus_tag="ACG8NZ_000725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0538424.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            12917..13390
                     /locus_tag="ACG8NZ_000726"
     CDS             12917..13390
                     /locus_tag="ACG8NZ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0538425.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            13392..13892
                     /locus_tag="ACG8NZ_000727"
     CDS             13392..13892
                     /locus_tag="ACG8NZ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0538426.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            13907..16219
                     /locus_tag="ACG8NZ_000728"
     CDS             13907..16219
                     /locus_tag="ACG8NZ_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0538427.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            16466..16783
                     /locus_tag="ACG8NZ_000729"
     CDS             16466..16783
                     /locus_tag="ACG8NZ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0538428.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            16780..16941
                     /locus_tag="ACG8NZ_000730"
     CDS             16780..16941
                     /locus_tag="ACG8NZ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0538429.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            16944..17243
                     /locus_tag="ACG8NZ_000731"
     CDS             16944..17243
                     /locus_tag="ACG8NZ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538430.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            17243..17557
                     /locus_tag="ACG8NZ_000732"
     CDS             17243..17557
                     /locus_tag="ACG8NZ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0538431.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            17550..18080
                     /locus_tag="ACG8NZ_000733"
     CDS             17550..18080
                     /locus_tag="ACG8NZ_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0538432.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            18077..18346
                     /locus_tag="ACG8NZ_000734"
     CDS             18077..18346
                     /locus_tag="ACG8NZ_000734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538433.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            18276..18479
                     /locus_tag="ACG8NZ_000735"
     CDS             18276..18479
                     /locus_tag="ACG8NZ_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0538434.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            18476..18763
                     /locus_tag="ACG8NZ_000736"
     CDS             18476..18763
                     /locus_tag="ACG8NZ_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538435.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(18796..19047)
                     /locus_tag="ACG8NZ_000737"
     CDS             complement(18796..19047)
                     /locus_tag="ACG8NZ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538436.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            19250..19663
                     /locus_tag="ACG8NZ_000738"
     CDS             19250..19663
                     /locus_tag="ACG8NZ_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0538437.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            20283..20447
                     /locus_tag="ACG8NZ_000739"
     CDS             20283..20447
                     /locus_tag="ACG8NZ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538438.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            20444..20674
                     /locus_tag="ACG8NZ_000740"
     CDS             20444..20674
                     /locus_tag="ACG8NZ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538439.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            20859..21404
                     /locus_tag="ACG8NZ_000741"
     CDS             20859..21404
                     /locus_tag="ACG8NZ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0538440.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            21501..21722
                     /locus_tag="ACG8NZ_000742"
     CDS             21501..21722
                     /locus_tag="ACG8NZ_000742"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0538441.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(21879..22043)
                     /locus_tag="ACG8NZ_000743"
     CDS             complement(21879..22043)
                     /locus_tag="ACG8NZ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0538442.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            22145..22666
                     /locus_tag="ACG8NZ_000744"
     CDS             22145..22666
                     /locus_tag="ACG8NZ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0538443.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            22653..23093
                     /locus_tag="ACG8NZ_000745"
                     /pseudo
     CDS             22653..23093
                     /locus_tag="ACG8NZ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(23133..23291)
                     /locus_tag="ACG8NZ_000746"
     CDS             complement(23133..23291)
                     /locus_tag="ACG8NZ_000746"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0538444.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            23384..24145
                     /locus_tag="ACG8NZ_000747"
     CDS             23384..24145
                     /locus_tag="ACG8NZ_000747"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0538445.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            24332..25684
                     /locus_tag="ACG8NZ_000748"
                     /pseudo
     CDS             24332..25684
                     /locus_tag="ACG8NZ_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            25700..26920
                     /locus_tag="ACG8NZ_000749"
     CDS             25700..26920
                     /locus_tag="ACG8NZ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0538446.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            26955..27632
                     /locus_tag="ACG8NZ_000750"
     CDS             26955..27632
                     /locus_tag="ACG8NZ_000750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0538447.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            27629..28822
                     /locus_tag="ACG8NZ_000751"
     CDS             27629..28822
                     /locus_tag="ACG8NZ_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0538448.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            28837..29163
                     /locus_tag="ACG8NZ_000752"
     CDS             28837..29163
                     /locus_tag="ACG8NZ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0538449.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            29167..29502
                     /locus_tag="ACG8NZ_000753"
     CDS             29167..29502
                     /locus_tag="ACG8NZ_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0538450.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            29499..29894
                     /locus_tag="ACG8NZ_000754"
     CDS             29499..29894
                     /locus_tag="ACG8NZ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0538451.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            29891..30262
                     /locus_tag="ACG8NZ_000755"
     CDS             29891..30262
                     /locus_tag="ACG8NZ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538452.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            30278..30838
                     /locus_tag="ACG8NZ_000756"
     CDS             30278..30838
                     /locus_tag="ACG8NZ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0538453.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            30963..31274
                     /locus_tag="ACG8NZ_000757"
     CDS             30963..31274
                     /locus_tag="ACG8NZ_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538454.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            31298..31489
                     /locus_tag="ACG8NZ_000758"
     CDS             31298..31489
                     /locus_tag="ACG8NZ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538455.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            31489..35337
                     /locus_tag="ACG8NZ_000759"
     CDS             31489..35337
                     /locus_tag="ACG8NZ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0538456.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            35338..36210
                     /locus_tag="ACG8NZ_000760"
     CDS             35338..36210
                     /locus_tag="ACG8NZ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0538457.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            36207..37256
                     /locus_tag="ACG8NZ_000761"
     CDS             36207..37256
                     /locus_tag="ACG8NZ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538458.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            37260..38084
                     /locus_tag="ACG8NZ_000762"
     CDS             37260..38084
                     /locus_tag="ACG8NZ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538459.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            38102..40162
                     /locus_tag="ACG8NZ_000763"
     CDS             38102..40162
                     /locus_tag="ACG8NZ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0538460.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            40179..40328
                     /locus_tag="ACG8NZ_000764"
     CDS             40179..40328
                     /locus_tag="ACG8NZ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538461.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            40325..40771
                     /locus_tag="ACG8NZ_000765"
     CDS             40325..40771
                     /locus_tag="ACG8NZ_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538462.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            40773..40910
                     /locus_tag="ACG8NZ_000766"
     CDS             40773..40910
                     /locus_tag="ACG8NZ_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0538463.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            40945..41187
                     /locus_tag="ACG8NZ_000767"
     CDS             40945..41187
                     /locus_tag="ACG8NZ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0538464.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            41203..41400
                     /locus_tag="ACG8NZ_000768"
     CDS             41203..41400
                     /locus_tag="ACG8NZ_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0538465.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            41411..42427
                     /locus_tag="ACG8NZ_000769"
     CDS             41411..42427
                     /locus_tag="ACG8NZ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0538466.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            42542..43750
                     /locus_tag="ACG8NZ_000770"
     CDS             42542..43750
                     /locus_tag="ACG8NZ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538467.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            43734..43904
                     /locus_tag="ACG8NZ_000771"
     CDS             43734..43904
                     /locus_tag="ACG8NZ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538468.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        15262 a         7403 c         9385 g        12107 t
ORIGIN      
        1 gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga agatagatta
       61 cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa
      121 tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac tacatcttgt
      181 gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta aaacgtatgg
      241 gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct cctcgtgatg
      301 gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca gaagtagttt
      361 taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct gacactgtac
      421 gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga
      481 aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg ttcgtccgct
      541 agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact tttacgagta
      601 caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc
      661 taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt
      721 gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca
      781 gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc
      841 gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat
      901 ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat
      961 caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggcttcca
     1021 ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga
     1081 aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca
     1141 atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc
     1201 ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc
     1261 aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt gagatcgaca
     1321 caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag
     1381 gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata
     1441 tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca
     1501 gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc
     1561 gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc
     1621 ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc
     1681 tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt
     1741 gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa
     1801 ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa
     1861 atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca
     1921 gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa agcacctgtg
     1981 gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga
     2041 catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac
     2101 aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta
     2161 ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat
     2221 gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc
     2281 gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt
     2341 gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga
     2401 atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg
     2461 cgattattca gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg
     2521 tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc
     2581 ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat
     2641 ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata
     2701 tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac
     2761 tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt
     2821 gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc
     2881 ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg
     2941 aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg
     3001 tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga
     3061 aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc
     3121 ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact
     3181 attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg agaagaaaac
     3241 catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt
     3301 ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat
     3361 ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac
     3421 agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg
     3481 tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct
     3541 acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat catcgttatc
     3601 agacagagaa agaacccttg gtattcaagg gttctttttt tattatctgt ttttttatat
     3661 taacgatttc tatttggtac atttttgatt aataaaaagg ggcaaaaaag gggcaacaag
     3721 ttacagtttt tctatatctt ctttcaattt ttcttttact ccttttgtca catgcaaata
     3781 aattttttca gtgatatcac tattttcgtg accaacgcga tcctgaatgg catacagagg
     3841 agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt
     3901 tttatcaatc ttcatgtcag ctttatgact tctcaaatag gtgttaattg ctgttagttg
     3961 gaaaggagtt cctttcgttg tttgaaatag aaattgacca tttggattta attctagaag
     4021 ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc cggcagctgt
     4081 ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca tcatcgtccc
     4141 atttataact actgatgcat tattattggt aatgtgtaca tcatctttac ttaatgcgat
     4201 tgcttcacca gacctcatcc cagtcaaata tagccactgg aaaaggagag aatatctttt
     4261 attgtgtgaa gttgtgaaat ctactagtct attatattca tcatcttcta aaaatttatc
     4321 tttaattttt atcgtttttg actctctttt ataatcaata actacttcat cgataggatt
     4381 tttttctaca taaccttttt tcatagcata tgaaaaaaga agattcaatt tggatttaat
     4441 tacactgacg tacttatttg acaagtcatc tttatatatc atgtcttcaa aaatattatt
     4501 caaatctatt gtattaatac cagaaacgat gtaagtctca ggtatcttct tctttatagt
     4561 gtttaatata ttattcgtag gataatatgt agattcctta acttgtcttt tgtatataac
     4621 taaccattct tcaacaagct catgaaatgt tagatcaggc ttatgaagtg ttttttcgtt
     4681 taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag tttctcgaga
     4741 tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat caatatatcg
     4801 ctcattatat ttatatactt tttttccgtt tttatcagtt ttagtttcga tccacattgt
     4861 tttcatctcc tatttgttgc tataatagga atagataagt aagccgatta tagcaggttt
     4921 atttttcatc acgtccacaa actttggcga gggaggggcg tgtttttgtt agttattgtg
     4981 gtaggttatc taaagcgtat tgtgcttgct catttgtaaa accttcaaat ataagttgat
     5041 cgtacaattg cgaatctgac attgatgccc aatcatcgta actttccgct ttagccaaag
     5101 cttgttcgtt gtaatcgaca tcaacattag ccagcgcata atcgattgca tcacttggat
     5161 attcttcaaa ctctagttgt tcacgcaaac cttgttcaga cataccagcc caacctagat
     5221 agctttcagc tttgctcaat gcatttctgt attcacgagg aacactttct tcttgagtaa
     5281 cttcttctat agtagtttca gtagtttcag tttcttcttc aacgactgta gattctgcta
     5341 ccgattctgc agaagacact tctgtcacgc tttctttagt tgtttcagcg acggtatttt
     5401 tagcatcatc actgcctcct tgcgaaccta tagcaataaa caccacaaca accgctaaca
     5461 accaaaacca tacccgttta taaaacggtt tcttaacttt atacattttc ccatcttgac
     5521 ccataacttt ttttgccatt taaatatccc tcgcttcttg ttataatata tttgtgatct
     5581 cagaaatgag gtatgagtcc gtgttgcagc acgggctttt ttctttataa cttttttaga
     5641 gattataggc aaaatagtag ggcataaaaa tgtattattg aattccgtat ttagaaaatc
     5701 ctaattgaac ttcaccggaa gtcttttgct gtgttgtacg caatgcttct tcttcagaca
     5761 ttccattctg tactttccat gcaacaggcg acatcccgta tttgttaaca aaatcagtaa
     5821 gtgataaagt gtcagcgtct tgctgagcgc ttgtttgttg gtcttctgga ttctgttgag
     5881 atgctgcttg ttgttctttc tgatcttgac tgataatatt gccagcatca tctgtagtca
     5941 atccattttc ataaagggcc acgccgaaag cttcccactc tttgttggac caatttgcac
     6001 gatcagctgg agttgactgt aaagtgcgtt gtttcatctg ttcatatgtt tcttcttgag
     6061 gtgcggtttg gattgtactc tgactggagc ttataactgt tgggctaggt tccgccgtag
     6121 cttggttgga gcttgtaact gttgaactag aatctgtttt agatgtagat ttgctagtag
     6181 atgaactggt ttcagttgtt tcttttgttt tacttatttt tgtttcttgg ttagaagtgg
     6241 tatctgttga ttcagctttt ttattatttg aacaagctga aagtagcaga gcagtactta
     6301 acaacaacat aacgctaact tttttcattt tataattcct ctttctcgtt gtaatatgtg
     6361 tgctaacacg ggctttttta tataagaaaa cgataagcgc tttctggaag cccgtaaaga
     6421 ttctttaatt cctcgatttt tttaggatat tgatcattat cttctttata aagagaaaca
     6481 atgagattag cagcaaagca attagcttcg ctttcagatt tgcttctaga tgttcttgtt
     6541 gatacataat agctggataa gccacgatga aaaatagcgt gacctaattc gtgagcgcaa
     6601 atgtagaatc tttcctcaga gtctcgcagt tcatcattta aaaagattat cgcacgatct
     6661 ctaatttctt gaaactgccc cttgggattc tcgataaaag gaacgtattg aattttaatg
     6721 cccatctttt cacaaatata aaaaggatta gcggactggt atttccgctt caactcctcg
     6781 actaaattaa tcgtatccat ctccataagc tcacatcttt ttgccttttt ctttgtcttc
     6841 tttcacaata tcccagaaag tcgctatcag gatatctttt acgcgctgta tttgttcggg
     6901 tgtcaatgtt tccccaccat aagacatatt aacatttgag tctagtagtt tatcaagttc
     6961 aaccacttcc tcttttgtag cccacttggg aacattatta tttcccaata aataatcagt
     7021 tgtgacacca aaataatcag caactttctt taagttctca gattttggcg aggctttatc
     7081 ccatcttctt atttgtccat tagagatgcc cacctgtctt tctacttctg ctatagtcac
     7141 atgcttttcg tctgctaatt ctttaatctt agtaactaaa ctcattatta tcaacctttc
     7201 aaagctgaaa agaaaataaa tagcttaaaa gttatatttt tggttgacaa ttagctttta
     7261 agctagtata tttaattcgt aagctaaatt gttagctaaa taagagcaac aaaaaactct
     7321 actaatttaa aacattctct cggtcgccaa acttagaaat gttattttag aggcttttta
     7381 taagtcttat ttaactatgt attcataata gcttaaaagc taataggtgt caacgattta
     7441 gctaattttt tagcttacaa attatttgtt tagaaaggag ctatttttat gtctgagaat
     7501 ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa agaactagct
     7561 gcgcttatcg gtatttcagg agcttattta tcagatattc taaacggcaa tcgtgatgga
     7621 aagaaagcac aacagcatat cgaaacagtg aaaaaaatat tggacatccg ataggggggg
     7681 taggcgatga caaaactaaa aaaacaagat tttgtaaaaa aatataatta ttctccatct
     7741 acttatcaac gtcgaatgtc ggaactaaaa aatacagcaa ttttctcagc ggcgtatgaa
     7801 cgggtcacag gacaagaagt ttggatcaat acagaattat acgataaatt tttgtctttc
     7861 aaatcctata acaggttacg cacaagaaag gtaacaccta aagaatttat cgagaagcat
     7921 ttagttgatt tataaaaaac agaacatttt gagaggtgaa ggttaatggg taaattcaac
     7981 agagcattag tattcagcgc accgctaatc gtctatgctt tagggctttg gggaagtaga
     8041 caagcgttga taggaacgat tgtgtacatg gtttggattt ttatagggct ggatgaagct
     8101 gagtacaaaa caaaaaagcc agtcgggagg gactgactaa tgagtaagga cgatattgta
     8161 tttctgttaa ttatgttttg tgggattgga tcaataataa ttaatttgta tttgattatt
     8221 aaagaaaaat ttttcaatta aaatgagtct tttagcttta acttaaattt tttttgataa
     8281 ttagctgttc ttatgaccat tacaagtttt tcaccaggta atatatcttt catatctaaa
     8341 acttctgaaa aagcgactga gtagccagga gagattgctt gtgccaacgg tataggagat
     8401 aaaaggctac ttttatgaat gcttcccata tcaaaaccat tggcgtgata atgtgaacaa
     8461 ttaattttct taccgttgta aagatacgta cattcgataa ttgagtttgg catttttgat
     8521 gggtttgcaa ttacgccagt aagaatcatt cgattacttc tatcatgctt aactaaaaaa
     8581 taggagagcg taatttttct tttatcttta aataagttat agagggataa aaaaaatcct
     8641 gaaatagaaa tgattaaagt aatagcgact tggtaatcgc tcaaaatttt tatcaagtaa
     8701 ttcaccacct ttttactcaa attatatcaa aaaggagaga agaaataatg caagaattag
     8761 taattttgaa aaataaaaaa agacacgaac gccggcaagc atgatcgtgt cagggaaata
     8821 aactatcttt ctatatttta ccacagaaag gaatgaaccg taaatgctaa ttgcaacgga
     8881 tacactagac cgcatctttt taaaagacga atacaaactg cgcaatatag atgcgtcagg
     8941 aattttagta tttgatcttt atgacaatgg aaaaattggt atctatcaag caagtgatat
     9001 tgaagaaaca aaccttgctt tcgaacaaat tgatgattct gtggaattgg atttagatga
     9061 ggcaatccta gcttttgaac aaattgcaaa attattaaag gaggcacaaa agaatggcaa
     9121 ctctttacca actcagcgag tcatatatca aagtcctgga actggcagaa caattggatg
     9181 aagaaattct tcgcgatact ctcgattcga ttaatgaagc gatcgagtat aaggcagaaa
     9241 acttagcaaa aatagttaaa gaagtagaag ggaaagctga gttaatcgat agtgaaatta
     9301 aacgtctgca ggaacgtaag acatcgcttt tgaacaatgc taagagtatc aagcactatt
     9361 tacaagagga aatggaaaag actggtaaaa cgaagattaa aggtgaatta ttcaacattg
     9421 gaattcaaaa taatccagta tcggtcaatg taatcaatga gaacttaatt ccaaaaggat
     9481 tctttacccc tgtgcctccc aaattggata aaaagcaatt gaaggaggaa ctgaagcacg
     9541 gagatattcc tggtgctgaa ctcgtccaaa caaaaggttt gaggataaga tgatgaagga
     9601 ggagaaataa tggaacacga aaacgtaaaa gacgctttga aagctgctat cgaaatcgcg
     9661 gaagctaaag gaataaaggt tgacggcaaa ccagcaactg tccatgacat tcaaaatttg
     9721 acgaaagaac atctttattt tatcgccgac ttgcttggtt tgtcggaatt gtatttagac
     9781 aaataacccc acacggaagg agtggctagg gtggttgtat tacaagtaat agtctgcgta
     9841 acattcattt ggtcgtgcat accggtttgt atatatatca tgaagaagct tcgccctgag
     9901 tcttacaaat tatacgcaat ttacagcata acagtagctc ttgcatttac attgtttgca
     9961 atttctaggc ttccgtttta atcgtgggaa tgaattttta atgaactaaa ttatctataa
    10021 agcaaaaaaa ggagaaacaa cattgaaaaa caaactagca aaaacaacag caatcatcgg
    10081 actagcacta ggtagcggcg ttatcggcta cgcagcaagc aatgcgtttc aagatttgga
    10141 cacgatcaaa gcaaatttca acacagtcct acaatacggt caaacgaaat cgcaacgtgt
    10201 gtcagaactt gaatcacagc tatccaacaa cactcgcaca caggagcagc tgaaagccga
    10261 aattgagcaa atcaaatcgg acaagcagaa ggaaattgaa gccaagcaac gagaagtcga
    10321 acaaaagcaa caggagatcg ctacaaagca acaggaagcc gatagcttgc gccaacaact
    10381 atccacagtg caaaacgaca aggagcagtt agaacagcgc gtgagcgagt tacggcagta
    10441 tacggatcaa aaggtagggg agttggggaa atgaaagagt tgactattta tgacccaaat
    10501 atttcatggg ctaaacacac aattaaagtt tcttttatga tttggggtta caaaggatat
    10561 gttacttaca aagtgggcgg taacactaag ggtttatcat taatagctat tgattcagat
    10621 gatctatatg atgctaattt cgaggataac ccagttaatt tccgtgacct tgatgaagat
    10681 tggttctcta tggaacttac taacgataaa ggcgacagta cacttgtaga agatgaattc
    10741 gacagactag gtgattatat tgtcggtgtt gaaattattg cgcacgaacc agagtaggag
    10801 agtgaataca aaatgattaa aagaatcact atatcagttg aagaagatag tggatcaaca
    10861 atgcagcata ccgacaatcg aaaaactttt ttaaccaata gaaaggagaa ttttgatgca
    10921 gattaagaag gcgacggata tcgaacaaac caagggaaca tatttgatct acggtgctcc
    10981 aggaaaaggt aaaacttcaa cggctaaata ttttccaggg aaaacactga tccttgatat
    11041 tgatcggact tcaaaagtcc ttagaggtga gaaaaatgtc gatatcgtat atatcgacaa
    11101 cgaagatacc tggaacgact ggggcaacac gttggctgat ttaactacca attatgtagg
    11161 tgtttatgac aacgtggttg tcgataatgt cagcgaattg gaacggtgta ttctgtccag
    11221 tttaggcgca gaaggaaaaa acaacggcgt tccttcgcaa ggcgattatc aatatatgca
    11281 atttagaatg gtgaacagcc tacgctatat gaaaaattta gacagcaacc ttatctggac
    11341 cgcatgggaa gaaatcgatt tgtggacgga ttcggatgga tcttcttaca acatcgcttt
    11401 gccgcagatc aatcgaaaga ttcgaaacaa tattttggga ttatgtgacg ttgttggtcg
    11461 attgatggta aaggaagacg atgaacgcgg atttatttta caagccacag attcaaccta
    11521 tgccaaaaat caattggata atagatctgg ctgtaagcaa agtgagctga ttttagatgg
    11581 aattgtacga ctaccagaat gatttagtcg atcgtgcaag acaagcctat gttgatggtt
    11641 acaaggcacc atgtatcgta tcaccgtgcg gttctggaaa atcagtcatg gttgcagaga
    11701 tcgctaaacg aaccacacga aaaggcaaca gagtgttgtt cttggtccat cgtcgtgaat
    11761 tgattgatca gatcaaagat acgtttcaaa agatgggcgt agataatcag cttgtaaatt
    11821 tcggcatggt tcaaacagtc gtacgacatc ttaaaaccat caagaaacca caactgatca
    11881 tcactgacga aaaccatcac gggttagcag cgtcctacag aaaaatctat gagtattatt
    11941 cggacgttcc gcgattaggt tttaccgcga cacccatccg gttaaacggt agtgggttgg
    12001 gggatgtgaa cgacttgcta attgaaggag tatcggctaa atggttgatt gagaatcata
    12061 gattagctcc ttatgaatat tatgcaccta aattgatcga tacagccgaa ttaaagaaag
    12121 cttcgactgg agattttaca aagaaatcaa tggataaggc agtgaagaat actatttatg
    12181 gcgacgtgtt gaaacattat aaaacgttag cagaaggtga acaggcaata gcatactgtc
    12241 acagtatcga tgcaagtaaa cacactgctg aaatattcaa cgatgctggt tataaagcaa
    12301 cacatatcga tgcaaaaact ccaaaagatg aacgtgcaga tattatcgaa gcgttcagaa
    12361 atcatgaaat taaaatccta tgcaatgttg atttgatcgg agaagggttt gatgttcctg
    12421 attgttcgac tgtaattatg ttgagaccta ctcaatcgtt atcattgtac attcagcaat
    12481 cgatgagggg gatgcgttac cgaccagata aaacgtcaat catcattgac catgtgggta
    12541 atgtgaaccg ttttggattg cctgatatgg atcgtgaatg gagtttagat acaaaaaaga
    12601 aaacaaaaac tgatagtgat ttatctatcg ttcaatgcgc gttttgtttt ggtgcttacg
    12661 aacggccaaa aggcgataat atttgtccgt attgcggaga gttgcagccg attgaagaac
    12721 gaaagtcaga gttagaaatc gatgaatcgg ctgaattaat gaaagtcgga gaaacgaaaa
    12781 ttactttgaa ttttgagaac aataaatatt acaacatgac ggaagatgaa gcagaaagta
    12841 tagaggactt atatgccata gcaagagcta aaggatttaa acctggttgg gcttatatgg
    12901 ctgctaagag gaaggggtgg ttgtaggaat gggcggtatt tttggtaaaa aagcagaaga
    12961 tgttcaaggt tcaataataa atggcgtaac aataataggt gatactggaa aaagagatag
    13021 ttcaggcaat caaattgtaa tcgctagaaa taaaaataac gaagtttttg aatcttctct
    13081 atctaatata aaaagaggtg ttgttaacgg gacaggcgaa gagagtggct acgttaattt
    13141 aaaaaaagct aatgaaaaat accatgttga aggaacttac gttaaggctt tgtctgtcgg
    13201 tggaacaaaa ggcgttaatt gggataaacg aagaaataaa tggaaagcac gattatattt
    13261 caaaggagaa tatgtcttac aaaaggattt caacaccaaa gaagaagcaa tccaagcacg
    13321 acttgcagca gaagaaaaat atttcaaacc tatcttagaa aaatatgaaa ataaaaagga
    13381 gaatgattaa catgacagga tttaacttag acttttcaaa agcgcaacaa gggaacgaaa
    13441 tcaaagacgg tacgtacgaa gtggtcgtaa acaaagctgt agaaaatgca actaaatcag
    13501 gcgcagaatt tatcgatatc gatttgatcg ttcgtaacga tgtggatcaa ccgttccaaa
    13561 ataaacacat tttcgcaaaa atctggaaag ccaaagctac cggcaaatat aacgaaggta
    13621 tgatcatggc aattgcacaa gcgctacagt tggaagacgg aaaatcatat aacgggtttg
    13681 atgaattgct ggcggacttt gttttgaaaa ccgcctcagt acgcgtgaaa actgaagagt
    13741 caaatggata taaaaacgtc aatgtgaagt cttgggataa aacgaacaca cggggcgtaa
    13801 tgaatcacca attcaaaaat ggtgatgaac catccttcgg accggaacgt tcaagagcga
    13861 caacggttag aaatgacaat cttccatttt aattgaggtg taatcaatgt atgaaaaaat
    13921 tccaaaagag cttaaaaatc tcaaacaatg gtgtgtttat aagctagttt gggatgaaaa
    13981 gcggcagaag cacacgaaga tcccttataa cgccaacaac gggcataaag ccaaatccaa
    14041 tgacgagagt acctggtctg attttcagac cgctctcgct gctattgatc actacggaat
    14101 gagtggactg gggttctttt tcaaaccacc ttatttcggc attgatatcg ataatgctga
    14161 aggtgaagtt gagcgattta aatcaggtga tatagaagaa aatattatct atgaatttat
    14221 cgaaagcatg agatcctatg ctgagtattc tcaatcaggt actggcatcc acattattgc
    14281 tcgcggagaa ttgcccggcg gaagacgcag gaaaggtgac gtagaaatgt accagaacgg
    14341 ccgattcttc gttatgactg gaaatgccgc atcaaaatat ttagaaataa ctgagccgaa
    14401 tccaaaggat atcaaacgcc tatatgatcg ctacgttgga gatgaaaaaa tcatccaatt
    14461 caaagaagaa aatccgctga tgaataccgt tgatttaccg gttgaagaaa ttatcaaacg
    14521 tgctgaatcg tcttcccaag gggcaagatt caaagtcttt atgaacggcg gttgggaagc
    14581 tttttattcc tcgcaatccg aagcggatat ggcgttcgcc aacgacttgg ctttttggac
    14641 cggtagagac tttgaaaaaa tggatgagat tttcagagcg tctgcaatga tccgtagcaa
    14701 atatgaccaa aaaaggggat cggcaactta cggagaatct ctactaaata aggcaattgc
    14761 tgatacaaat tccgtctaca accccaaacg gaaatctgat tttaagattt ttatcaaaga
    14821 ccaggaacag ccaaaagagg aaaaatacta ttcgtacgac gacaccggca acgcggatcg
    14881 gtttactgat atctacggaa cgttagtcaa atattcgtac attgataaat cttggtatta
    14941 ctacgatggg aaagtttggc tacaggacaa taccggcgaa gttcggaaaa tgatcgatac
    15001 gacagtagac atcatgggaa aagaaccgtt aacgatccct gaaggcgcag acgatgaaac
    15061 caaagaagct ttgatgaaag cgaaagaaaa acacgtcaag cgttctcgca gcaatgccgg
    15121 aaaaaacgct atgatggacg aattgaaaca tcgactatcc gtattgccgg aagagttcga
    15181 caaagataaa acgctgttca acacacaaag cggctatctg tctttgcatg acggtcttct
    15241 acacgagcat gagatagaca aaatgtttac acgtgtttca aatgtcgagt acacagaatc
    15301 ggtcgattgt cctatgtggg aagaatttat taatcagatt tttgacaatg atcaagaatt
    15361 aatcagatat atccagaaat gtgtcggata ttccctgacc ggatctacca gagagcaatg
    15421 tatgttcatc ctctacggtc atgggtcaaa tggaaagtcg gtatttttgg aaattatttc
    15481 tgaattgatg ggaaactatg cgatgactat gcaggcacaa acgatcatgg tcaagcaaag
    15541 ccaatcatca gcaaacagtg atattgcacg attgaaaggt gcgcggttgg tcacttcgtc
    15601 agaaccgaat gaaggcgtcc gactggacga aggtctggtc aaacaattga ctggtggtga
    15661 taaagtcacg gctcggcacc tttatggaaa agaattcgag ttcgaacctg aattcaagtt
    15721 gtggctggca acgaaccaca agcccatcat ccgtgggacc gatgacggca tctggcgacg
    15781 gttaaattta attccattca ctgtccaaat cccggatcac aaaaaggata aaaatctgaa
    15841 atttaaattg cagacggagt tgcaagggat ccttaaatgg gcaatcgatg gttgcctgtt
    15901 gtggcagaga gagggcttgg agaaaccaaa gtcggttgtt gcagcgagtc aggattatcg
    15961 aaacgaaatg gatcaaatag gtacatttat cgaaacgtgt tgcgaaacag gtccaggact
    16021 aaaaatatca ggcggagaat tatacaaagt ttatcgtgaa tgggcatcag ataatggcga
    16081 gcatactttc actaacacga agtttggcag agagatttca aagaaatata gcaaagaaaa
    16141 agctggtgga tttatggttt ataaaggaat cacattaaaa ccaagaaaat acgataacgt
    16201 tagagaactt tttaaataaa tctggagggt tctggagggt tgagaacgaa accctccaac
    16261 ccttagaaac gttgatatat cagtatttat attatatttt tctttctttc tggagggttt
    16321 taaaaaaata ataaagtatt aaagtaaaaa ataaaaagta aattatataa aaaagaatag
    16381 gtttttgggt ctgaccctcc aaactctcca ccaaaatctt cctaaatgct ttgataccaa
    16441 cgattaaaca gtctggaggg ttacattgaa atcagaacat caaatacaag acgagatcag
    16501 aattgcctta tcaaaacatg gttgcacgat ttttcgatcc aatgcgggac aagtgacaac
    16561 gatcgaaggg cggcaatttt acgggatgcc aaaagggttc ccagatctat gtggacatcg
    16621 taattctgat gggaaagcaa tttatataga agtaaaaaat gaacgcggta agttgcgtga
    16681 agatcaaaaa aggtttgctg aatttttaaa cggtcaacct gtactatacg gtgttgcacg
    16741 atcagtggaa gaagcattga aaattgtgga ggaatcatca tgacaacaga agaagtgatt
    16801 caaatgcgta ttcgaagcct tcagcgtgag attgacgatc tggaacggac aaaggcagtg
    16861 atggtcaatg aaacggcgag gaaggcaatc gatttgcaca tagagaattt aagaagggaa
    16921 atccatcgat tggaggaatg agcgtggata agaaagcagc aatgaaacga atcatcgaac
    16981 tgacacattc tgagaattgg caagaagaca aagaaatagt tgcagaagtc caaaagctcg
    17041 gtaaatcaat gtggagtgaa aaaaccaaac ggagaacacc gagaaaaatt gcaatctggc
    17101 atggtgaccg aattctagta acaggtactg ctgaacagtt atctgaaatt actggattaa
    17161 gcaaaaacat tatctgggat agagctaaac gcgagaacgt tgattcgaaa ggtcgtcaat
    17221 ttaaacattg ggagaagaaa taatggagga actaatcaca aaagtagagc agtgggcaaa
    17281 agataaggga ttggatcaag ctgattccag caagcaaatg ttaaaaacga tcgaagagat
    17341 tggggaagtt gccgcttctc tagctagaaa agatgaatat ggtttaagag acggaattgg
    17401 agacgtagta gtaaccttga ttattttagc tatgcaaaat gatatggatt tgtacgagtg
    17461 tctgaaccaa gcatacaacg aaatcaaagg acgcacagga aaaatggtag atggtgtatt
    17521 cgtgaagtcg agtgatttgg aggacagcga tgaataaaaa agtattaatt gataaacaag
    17581 cattgattga tgaattaatg aaaatacctg gtgtgggatc taatagtgac gctttagaaa
    17641 cgattaaacg tttcccatcg tatgaaccgc agaagccagt tgtgcctaag tttgtggcgg
    17701 aactactcga ctattatcgt cagtcaacag atgttgattt attagcttta ttgataactt
    17761 tccatgattg gtattatcgc aaaactaaag acggtgagca tgaagaagca attgattggc
    17821 tagttgatca tcctgaaatt tatatgcgtg catggctgtt tggctacgag ctcgagaaag
    17881 agccgttgta ttgcgtaata atcaatggta gatatcttgt caaagtattc agcaatacaa
    17941 acgttgtcat attagttcca gcagatgaat tttcacagta cgttactcaa aaatttcaat
    18001 taactgaaaa acaaatcaaa gcaattgatg aaagatactg gccgtttgct gtgccagtgg
    18061 atgaggtggt agaaggatga gcgacccata cattactaaa ttagaaaagt tgtccgaaga
    18121 ttttgagaag aataaaaata aactaactaa agataaactt atagaactgg ttaggttggt
    18181 tatcagagga gctattacaa gagaaaaggc gcagcaaaac atagaaattt ttgctcgtag
    18241 tgtccgagat ggattagaga agaaagttaa acaaaatgaa caacagacac cgcagaataa
    18301 caaaactaag aaaacgggaa ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa
    18361 tttcagcaga agaaacatat aaagtggcaa gtcagtgtgt tgctgatgcg agcggcgcta
    18421 ttcgtaagtt tggaatttcg atattaagtg atgatcgtaa atgggaggaa aaagaatgaa
    18481 actaaaagac ggattttact ccagcagtca tggtatcggc ggtttaatgc tagatatgcc
    18541 gacaaagaac cctaaaacac gtgagaaacc aaaattcaaa gtcggtgaca tggttcgctg
    18601 tgaagcagaa ggattcatct atccattttg tggatatgta gaacatctct ataatcactc
    18661 agcaatcatt cgtattgaaa acacgatgga atgtgacaag tggttagcga aaagcaaaga
    18721 gaatttagca gtagcgagat tggtggatat ggaggttata taaacagcga aaaaatgtac
    18781 ttctaagaga aaaatttatt ttttataaaa ataggaaagt atcattatga aaaagaccac
    18841 agagataaat aaacctacca cataaaaaat gaatatttct tgcgattcaa tataggtata
    18901 tttaacaatt tgtacaataa aaaatactat aggtaaaaaa gataacatga ggagcttatt
    18961 tgttctagag tgtttgacaa agtaacaaaa tgcgacgata ccaatacaaa cgggaatcca
    19021 aaataacagc atgctccaca tagtcactca accatctcct atacgcttta ttatcaatag
    19081 ataatatcat ttttcgtaat aaagataaaa aaataagtaa ggcttctcat taaaatataa
    19141 aaaagacagc cgaccactga ctgcctatat aagagtattg aaataaaaag ctgctgatat
    19201 aataaattcc acaagtttat tatatcacat aaaggagcgg tttgacttga tgcaattgtt
    19261 acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg
    19321 gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga ttattacgga
    19381 tatgccgaag gcgccgaagc acggcaataa ggcagaagac gcaatcattc agatgatgga
    19441 tatagaagcg gagagagacg cgattctagc agccttgatg gctcttagtc tgattagtcg
    19501 tcagatactc tactatagct tttgtgtgcc agatagcttc tcaaactaca gaattagccg
    19561 tgaagtgggt tattcagaaa gaagtataca acggatgaag tcggaagctc taatagagtt
    19621 tgcagaagca tataaacacg gaagaataat tgcttataaa taatttggcg gctttttggc
    19681 ggaatgatgg cggtttttag ctatttacca gtgatattat ggtagtgtcg aaagattagt
    19741 gataggtcta agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta
    19801 agcttcgata gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta
    19861 aattgttctt attatctatc atccgttgct gtctattgtt tttttaatta ttcacacgat
    19921 aactaaaggt gagcgaagag aaatgattcc attaataatt tcaatttttg cgctttgtct
    19981 taatgtctat atgattggat ttaaaaatgg gcaaaataaa agatagtagt aactaagaaa
    20041 gaaattttta tattgtcact gtggcggaaa gggtagacgc taagcatgtg tgctaggtca
    20101 atgcttcggc aactatgcaa tgttcgattc attgccagtg actttggcaa ccgaggcatc
    20161 ggcggtttaa aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg
    20221 ctattacata ttagatcact cgttgagtgg tctttttatt ttacataaag gaggtaacaa
    20281 cgatgtatag accacaatac ttagaacaga agtatgaagt aatcactgtg caaaacggta
    20341 acggtgagat agtacgaaag tatagaagac caataaagag cgatacatat aaacgaaagg
    20401 aaaacaatga agttatttct tttcgcagaa ggagaaaagc caaatgagaa actactggta
    20461 tgtatcgcta actaatgaat atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg
    20521 ttctgtacaa atcaaaggga agtactctat tgtcgaaatg ctaagagaag ctacaccaaa
    20581 cgaagtggat aaatgcaagc tgatatattg cggtcatggc tattggaaag acgagtatat
    20641 ccaacaaaac attgagaggt ggatagatag atgagttacc tcgaacattt gaaacgttgc
    20701 tacatgcatt ctaagaataa acttcctgac agctacactg tagatgatgt tgctatacat
    20761 gtattgaaga ctgagagcca tagcagtcct gatggtagca gtaaggagca gacgctcgcg
    20821 tggttcaagt tctttaagtg gattaaggag gaagagtaat gcacatgcac ataggagaaa
    20881 agatatataa attgagacgg cttaataacg aaacaatgaa cgacttagtt aaacaaactg
    20941 gagtgtcaaa atcatggtta tctgatattg agtctggaaa aaagcaacgc gttgatttta
    21001 ataagatatg tgcaatagcc aaacattata aagttagctt agaatacttt cgggatgatt
    21061 atttggatag tgatgaacac gaggatatta ttcctttgac aggcgaaaca agagttaaat
    21121 cagtgaaatc gcttgaacaa agcctaaagt atctgaataa caaacaagaa cctaaccttc
    21181 tagagatcaa actaaaagat actgactcag taccagaggt ttggtacaag ggtgagaggt
    21241 tggatgaatt gcctaaagga ttagtagatg tctcgtatca ttggaagact gatgatttta
    21301 ctaatgatga tagaggagcg aacgacatca cgattcaata cttttctagc tttaatgata
    21361 agtatccaga taagaaaaca atcggacaca agagagatat gtaaatgaaa gaagttagac
    21421 ctagagacga aatagacaaa ctatacaaga ccaaacgatg gcgagaccta aggcaagtag
    21481 taatagctag ggacttcggc atgtgccaag agtgcaagcg tcgagggcgg aacacaaggg
    21541 gcacgatcat ccatcacata gtcgaggcga gggaagacct gtcactgttc tggtccgtag
    21601 ataaccttga atgtatctgt gtagcttgtc acaacagaga gcatccagag agatcaggcg
    21661 ggaagaagaa accaaaacct aaatcacata tcgttaaaat gtattcaact cctgaaagat
    21721 aagtttgcag cgaaatgaag gtagcccccc tactctaaaa gattaaagag taaggcttga
    21781 gaagaacggt gctgtccttc cttcgtaaaa agaccgcttt tcaagttttt tggagaaaaa
    21841 ggaaaaagcc gaccaattta agccggcttt ggacgaagct atttcttagt ccatttgttt
    21901 cctttttgag aagtaggggg taatcggtcg cctggatcaa tttttacttc ttgtccgcct
    21961 tggacatttc caccacgagg acccacttct ttgtaggttc cttttggttt gttgtcttcg
    22021 ccgggtttat agagttctcc cataggaatc cctccttaac aaatttcggc acagcactgc
    22081 cgataaccta attataagga ttgtgataac gatttcaatc tatcttttga aagaaggtga
    22141 tattatgccg caaccagcga agagtgcaaa attacaatta ttaaacggaa acccaaataa
    22201 gaagaatacc gaagaactcc gcaagcgagc ggccgcagaa gacaaattaa aaatggctac
    22261 tgacaaaatc aaaccgccgt tatggctaga ttcgctagga aaggatacct ttgagtttat
    22321 cgccgatgaa ttgctgtctg tggatttaat cagtaatccg gacgtccata caatggctct
    22381 atactccaat tggtattcgc aatacgtttc tttagaaaaa cagcttcgaa aactacaacg
    22441 agagtacaag ttgaactatg cgcttgcgaa aaaggaggca gaggcgagag gtgagccgtt
    22501 taatgaacct aatgaattaa ttggtaatcc gctctctcgg cagatggata cagcgtctcg
    22561 gaatctccgt tcttttggcg ctgatttagg actatcacca gcagccagag ctaagttagc
    22621 tattaagatg gctgatgatg gtggtgatga cgatgacgac ttctaatatt ttggatatgt
    22681 cttacacaga acgtgtggac tattggcaaa gctatcttga ggagcaagct tcttggggtg
    22741 gctttttaaa atgtccatat ccggaattgt taactacttg gtatgcggaa cgattaatcg
    22801 atggaagcat accagccagc aaagaaaata ttcaagctgc taaacggcat atgcgtgatt
    22861 tgcagcgcca aggaacagat gattttcctt ggatctttga cgaagaaaaa ggtcaccggc
    22921 ctattagata tatcgaaaaa aaatgtaaac caactgaagg cgactttggt tcgtttgttt
    22981 tgcaaccttg gcagcatttc ataattggat ccatgtacgg atgggtacat cgtgatacag
    23041 gagagcgtcg cttccgcgag gctcttattt ttgttggacg taaaaacggt aagttttgcc
    23101 gtttaaaaat cgggcaaaat cggtgaaaac ctttattttt ggctttcttt tagtaataat
    23161 tcgatagctt tatcaagcaa tttagacata ggaaccatag tttcttccga catttttttt
    23221 aatccttcgt ataaatcttt atcaattgcg ttagaaattc taattctgtt ttttagtccg
    23281 tatttgtcca tgtgtgacac tcctttttat tttactataa catccgattc caccgcttgc
    23341 aactaccatc aattgatggt agtatataaa agaggtgagt agattgaaaa aatacattat
    23401 ttacaaacac ttaaataaaa taaatggcaa aatttatatc ggtgtcacaa atgatattgg
    23461 cagaaggtgg aggagcggtg ggattgaata taaaccacca aagaacgaaa cccaacacca
    23521 cagaagtttt tggaatgcaa tacaaaaata cgggtgggaa aattttgatc atttgattat
    23581 tgaagaagac ttaacgatga aagaagcttt tgagaaagaa aagttttata ttgaacttta
    23641 tgactcaact aataagaaaa aaggttataa catcgcaaaa ggcggaaatg gcggaataat
    23701 atataaagtt caccccaaag gaatgctagg gaaaaaacaa tctaaagaat tttcttcaaa
    23761 tcacagtaaa tgggcaaaga accataagaa taattgtatg acaaacggtg atgttgtatg
    23821 gggtgttact cacgaacacc caaaaggcat gttgggaaag caccacagca aagaatcaat
    23881 tatgaaaaag aaagcatata gtggtgaaaa tgctgtaacc tcaaaaccaa ttgtcgcaat
    23941 tgaacgggat ggaactaaaa gagaatttca cagcgctaag ctttgcatgg cttactattc
    24001 gattagtaca tgtgtttttt accgactttt aaaagatggc gccccttatg tgataaatcc
    24061 taaagcgaac tacagaaata aagaaaaaat attagctatc gaaggaatta tgttcaaaca
    24121 taaagaagat accgaggtaa gttagtagat tgcgaaaggt tactaactac cgtaacgcgt
    24181 aggagttgaa taaatataat gctcccaaga gtgtccgaca gcagataatt tgctgaaaat
    24241 gtacgctaaa ctgggccaga actgactgac cgatgaaaat gagggtgacc tccagagtgc
    24301 gagataaaaa gctcgcagat aataacaatc gaaaacatca ctaatttcag gcctttccac
    24361 atacatggtc gcttatgatg atgaacaagg cgccaacgtt tacgtattgg caaatgctcg
    24421 tgatcaagca agcttgttgt ttgataaggc cgcagaaatg gtcaaacaat cgccggcgct
    24481 ctttaagaaa tttggtaagc ctaaacgatc aagtattaat tatgctcccg ccttttctaa
    24541 aatggaacca cgcgcctcag atagccggaa attggatggg ctaaacactc actttggtat
    24601 ttttgacgag atccacgagt ttacgaatta caatctgatc aacgttatca agaaatcaag
    24661 aggaaccaga aaacagcctc tgatagttta tatcacaact gctggatatg tattagatgg
    24721 tccgttgatg tcttattttg agcaaggtgt ggactgtttg gaacatttgg aagatgacat
    24781 cgatgaacgg actttctatt atctggcaaa acttgacagt gcggaagagg ctgatgaccc
    24841 aagattatgg atcaaagcca atccgaatat ttgtctaatg aattttgttg gcatgctaga
    24901 tgactatgtt aaggataaaa aagatccaaa agaatatgct gactggatta ccaagcaatt
    24961 taacttgttt tccgatatcg atgagctgtc atttgtcgat atgcctacca ttaaacgaaa
    25021 caataaaacc atcgatattg aaacgctcaa aggtaagaag tgtgtcggtg gttttgactt
    25081 gtccgaaacg gaagacttta ccgcagccgt tttagaattt ccgcttgaaa caggcgaggt
    25141 attcattttg caacacacat ggatcccaca agctagattt gatcgagata acaatcaaga
    25201 gcgtatcaaa gcgtgggaga aggtgggaga tctaacgatt attcctggtg attacgtcaa
    25261 ttatgaatac gtcttaaatt ggtttgtaga aaattcgaaa atctatgaca ttgtaaaaat
    25321 caattatgac aaggccaagg cgctacgatt aaataaagaa ctagaaaatg caggatttga
    25381 aaccaacgag attcggcaag ggtttctatc attaggtggg ccaatgcaaa acttcaagga
    25441 aatgctattg gacggtaagg tgattttcaa caattccaag ctttaccgat ggtatctatc
    25501 caacgtcaag ctggtgatgg atcgcaactc aaactggatg ccgtctaagc agtccaagag
    25561 tagaaaaata gatggttttg cagcaagttt gaacagccac gccgaagtgt tgaatatgtt
    25621 ggttaatcct gtcggaaccg ggaaagtaac ctattactcg atttccgatt taatgaatat
    25681 gtaagaaagg tgtggaggaa tgagtatttt agatcgtttg cgttcttttg gccgagcgaa
    25741 gccgaaagcg agcaaacaag agtatttttt gaatgacccg ggattgatac cgtatttagt
    25801 cggaaaagat gaaatatcag aagggatttt ttccgtaatt agccgtgtat cgaacgtttt
    25861 tgcgtctctc cctctcaaaa tgatagatgt ggagtttggc caaccggacg actgtcctgc
    25921 atacaacttg ttgagcgaag gccctcgata ttttacaaag tttgattttt tccgggacgt
    25981 ggaagttttg agaaactacc aagggaatgc gtatgtgcag attttccgaa atatcaatgg
    26041 agaagtagca gatatggcgt tagtaaaacc tggtgcttgc catccagtga ttgatatgga
    26101 tagcggggag ctttactacc aagtaactgc gactgacaaa ggcagttaca agcaagttat
    26161 ctatgtacat tacatggaaa tgcttcactt taaacaaccg aggtttggcg gcttggaagg
    26221 tacagacccc acaaaagtat taacgaatac cctcggatat gatcgagaag tccgaaaaat
    26281 ctctttaagt cagcttaaag gaagtaatga agggctaaaa gttaagtttg ctagcaatat
    26341 ggatgaagaa gctaaaaaag ctacagttaa aaacattgct gatttttatc gacaaaacgg
    26401 tggactactt gtggaagaaa acggtgtaga aatcgaacgt ttacaacgag agctggtaga
    26461 cagcaagctt ttagatactg ataaagtatc tcgctccaga atcgctatgg tctacaatgt
    26521 gccggagcat ttcatcggga ataaccagtc gagttactcc tctcaggaac agctcaatat
    26581 ggaatttttg acgtacaatc tagtaccgac cgttaatcaa tatgaagcgg aactaaataa
    26641 gaaaacacta tcgagaaccg aaaaagcaaa aggttatcga tataagttta atgtcgcaag
    26701 tttgctaaga gctgacactc aggcaagggg acaattctat caaatcatgc gacgaggcgg
    26761 agcatactca gcgaatgatg tccgaagatt tgaggacttg caaccgataa ataaaaccgg
    26821 tatggatgat taccatattt ccggagacct atatccaatc gatatggatc caacattaag
    26881 aaaaacaacc tcgtctaaaa gcgtagccga aaacggttag gtttttttag tttgcaccga
    26941 agggaggtgg aaaaatgaaa aaagtgacgt taagcggcga tgtcgtggat aacgataccg
    27001 cgtggcttta tgactggttt gggatcaatt gtatctcacc agggaaaatt tctgccgctc
    27061 ttacagaagc agcgggggat gaagtagaac ttgatatctc atcgaacggt ggggatgtcc
    27121 tagcggcaag cgaaatatat accgctatcc gtgcctatcc gggaaaggta tccggaaatg
    27181 ttgtgagtat tgcagcaagc gctgcaagtg taatcgcttg cgcttgcgaa ccgcttagaa
    27241 tctcacctac ggcacacatc atgattcata acgcatgggt gaccactagt ggcaacgctg
    27301 aggaattaaa agccaatgca gaaatgttaa gcagtgtgga tgagtctatt gttaatgctt
    27361 acgagatcaa aacaggactc gatcggaaaa aacttgctga tttaatggcg aaagatactt
    27421 ggttaaatgc tcaaacagca gtagcagaag ggtttgcaga tgaaattatg tttgcagaag
    27481 caccagtaac ggtactcaat gcctctcaac cggttattcc aaaaaacgca gtaactaagt
    27541 tgaaaaattt aatactcaaa gcggaagcac cgcaagaaga aacactctta cagaaaaaac
    27601 taaaagcctt aaatggaggg aaaaacgaat gaatttagaa caattaaaaa atgcgtgggt
    27661 cgaggcggga agtaaagtct ctgacttaaa tgcacaactc aatgcagcat tggttgacga
    27721 tgaaaaaaca gaagaagatg tagtaagttt gcaagcacaa gtaaaagcag cacgggctaa
    27781 acgggacgga ttgaaagagc aagtggcaaa tatggaagcc gaacaagtct taaacgtcaa
    27841 aaaagaacca ttagataaaa aagatgaaaa cttgaaaaac aagtttatca aagactttaa
    27901 agcgatggtc aatggtgatc ctgctattat ggctactttg acatctaata cggatgaatc
    27961 tggtaatgct atcggattga ctattcctgt agatgtgcaa acgactattc atactttggt
    28021 tcgtcggttt gactctttgc aagaatacgt aaacgttgaa aaagtgacca ctaccagcgg
    28081 ttctcgcgtt tatgaaaaat ggtctgatat tacaccgttg actgctttgg atactgaaga
    28141 cggtgaaatc ccagcaaatg atgatcctgc actttacttg atcaaatact tgatcaaacg
    28201 ctatgcaggt atttccacag taactaacag cttgctaaaa gatactgccg aaaacatttt
    28261 ggcatggttg tctaaatgga tcgcgaaaaa agtagttgtt actcgcaata caaaaatctt
    28321 ggcagctatt gatggaatca aagcggcaca aaagaaagat gttacagatg ttgatggaat
    28381 taaagatatc gtaaacgtcc aacttgatcc agctatcgaa gctacatcta tgtttattac
    28441 aaaccaagat ggcttcaatg ttttagataa agtgaaacgt gctgatggat cttacttgtt
    28501 acaaaaagac gtagcttctg caactggata taccttctta ggtaaaccaa tcaagaaaat
    28561 tgcttctcgt ttcttgccaa acaaaggtac gcaagctact cctaaatatc cactgtacat
    28621 tggtgatctg aaagaagccg ttacattgta tgatcgcgaa aacatgagct tgctgacaac
    28681 gaatattggt ggtggagctt ttgaaacaga caccactaaa gtacgcgtca tcgatcgctt
    28741 cgatgtgcaa ctagttgatg atgaagcggt tgttttggct acttttacaa ctattgcgaa
    28801 cgagacaccg gcgaaagttt aaggagctga ttccttatga ttcttgatcc taaaatggat
    28861 ttaggcgaaa tcaaaaacgc actaaagatt gataccgatg atgacgatgt ggaagtaagc
    28921 cgtgcggcac aagctgcaat tgcatacatt agaggggcta tcgggaatga taagccctct
    28981 ttttataagc aagaaaatga aacggttgat ctgattaatt tagctatcct gcaattagca
    29041 gatcattatt acaacgctcg atcagcaacc gtgagtggga acttgcgaga gtacgattta
    29101 ggttttacaa gcctaatctt gcaactcaaa gcaagttatt tgctttttgt ggaggaggag
    29161 tagcgtatgc cccttatcca aacaggaaat ttaaatcaac gcatcaagtt tgtccgagat
    29221 acgacggtta aggatgagga cgggcaagtt gtcccgactt ctacaaccat tcttacttgc
    29281 tgggcaagtg tgcagacaca acgcctgaac gatattaaga cgtcgattgg tacggctttg
    29341 gaaggaacac tgacgttcat tatccgctac caacaaaaat cagagctgac caatgatatg
    29401 aaagtgcgtt ggaatggaaa aacgtttgaa atcattacga ttacgaaagg cgagtttgcg
    29461 aaggacttca cgacaatcat tgcaaaagag gtttcaaaat gagtgtagaa gtcgatgcaa
    29521 ccgaagtgta caaagcgctt agggaagtaa aagcaaacgt tcaacgagtg gaaagcccag
    29581 cacttagaaa agctggggag tacgctcaag aagagttacg acaaaacaca ccttactggg
    29641 atggaacgaa gtcaaacggt aaacgtggtt cgtatatgca agaacatgct aagaaccatg
    29701 tggttacaag ctcgataaaa aacggattgg tagaagtcgg ctatgacaaa gatgtttctt
    29761 ggcggatgca ctttatcgag ttcggaacaa tcaaacaacg tccaaaaggt ttcgtacaaa
    29821 aaacacaaaa gcaaatcgaa aaacaagtaa cacaaatcat tgctgacgaa gtaaaaagga
    29881 ggctaggact ttgaaaacgg cagtatcaca agtctattca attctgaata gcaatgaaaa
    29941 aacaaagaac attgattttt acaccaatag tgttccggaa tcagctcaaa cagtacctag
    30001 ccttccagtt ggcagaatta cagagatatc cggcaactat gaagattttg caagcaacaa
    30061 tcctttgacc attcaattta acgtacaggt agatgtatgg gtgtcaacca tgaaagaggt
    30121 tgatgccttt tattttgccc ttgatgaggt tatgaggggg aatggttggc aatgcgcata
    30181 cacggaacaa acagatgacg aggacttgga aggtgcaaag cggattatca aacgatatgt
    30241 agcaaatatt tcactaaatt aaaaggagag aaaatagatg gcaacagtag gattcgagag
    30301 cgtcattttt ggcgtaaaaa caggtgcagg cggcactcta aaagaattag tagcagataa
    30361 gtcgaaaggc ggagcgatcg aagctaaaat tactggatta ggcgcaactt ctaacacaac
    30421 atacgcttca aacgtaccgt tcttcattgc aagtaaaggg gtttcgtcgc caaaagttac
    30481 gcttgacgtg gcagacttaa tggataacgg catttacagc gaaatcattg gtgctaaaac
    30541 cgtggatggt gcaaatgtaa ttggttcaga aactgaagcg ccttacgtgt cggtagtcat
    30601 ggttacagcg aacaaagaag gaaaacgctt gttcatggga ttgacaaaag gaaaattcag
    30661 tcatccagat atcgacatga aaacagctga agacaaaggg gtagaattgc aaaccgattc
    30721 catcgaaggg gaattcattt ctgatgaacg tggctatgta tacttaacag ccgtagaatc
    30781 agaaggaatg accttacaaa aattcaagga cttggtaaat aacaaagagg gggagtagtt
    30841 aaccctgcat ctacaccaac gacagataca gggacaccaa aagaaccaga accaaaaatt
    30901 gatacacaag gttagccatt tttggctagc cttatttttt gtaaaaacaa ggaggaaaac
    30961 aaatgattga attgcaattg aaacttgacg gaaagaaaaa aacattcaaa caacaagata
    31021 tttccgcacg tgcaatgcgt gagtgtatca aattttacga gaaagcggaa aaagcagacc
    31081 taactgattt agaagcgatt gattcaatga ttgcaattac agcagatatt ttccaagatc
    31141 cagcagttac atttgatgct attttagacg gtttgactgc gagcgagtta gtaccggcat
    31201 tagaaagtgt ttttgaacaa ataaatgaac tgggaaacaa tgaaaaaaag cagacggcga
    31261 gcaaaaagaa ataagttttt ctgaagctag gaaagcaatg gatcaaatct acaaagattt
    31321 aatcgaagca ggttggacga tgagagatgt ggacgaagcc gactatcatt atttgttaca
    31381 cctttttgga gaagtggaga gtggcgaaga atatgtagat ggtgctgatt tcatcaaaca
    31441 atttttatcg gctgaagact tagtaaaact tgaggaagga ggtaaataat ggcaggaaaa
    31501 ggacaaccgg caggaaatat caagctaggg attagtttag atagcactag ttttggtaac
    31561 acgctggacg aaatcaatgc gaaagtcaaa caagctgagt cgaatatgcg tgccaatcta
    31621 aaggcttatg attcagcagg acgttcatac gaagcactta gtcaaaagac gaaagacttg
    31681 tctacggtta tggaagggca aaacgccaaa gtaagagaat taacaaagcg ccgtgatgaa
    31741 gcgattagca agtatggcga ggaatcgaaa caagttgcta accttaacac acagataaac
    31801 aatgctaccg caaaatataa tgcttacagt cgccagttga acgacacaaa aaaagaattg
    31861 gtgtattcca aaacagccgt caatgattta tctaatgaaa tcaaagaaaa tgaacgacaa
    31921 atgaacgccg aagtaaaagc gttgaaagcc gctggtgatg aatctggtgc ttttgaagca
    31981 aaacaaaaag ggctagccaa acaaacggaa ttatccgaga aagctatcga agaacagcgc
    32041 aaagttgtga aactgatggc tgatgagttt ggcgattcag caaatgaaac cgaagatgca
    32101 aaaagggcat tagaaaagtt agaacgacaa agccaaatat ctagcaggca attagaagca
    32161 ctcaaaagct ccagcgatca atcaggaaaa gaaatagaag attttggcga caagtccaca
    32221 aggtcagcta ggaaactgga cggactaaaa gacaaattaa gctcgctaaa aagcgcattt
    32281 tcgtttggtg cagttgctgg attagcgcat aacgctatta gcagtgtagt aagtggcgtg
    32341 caaggtttgg ttggcgaagc agtaaacgca tcggattcat tgatgaagtt ttccaaaacc
    32401 atggagtttg ctaactttgg gaagtcacag atagaaagct cgaaaaaaga aatgaaagac
    32461 tacgccgata agacggttta tggtttagaa gaaattctga acacaaccgc acaattggca
    32521 tctaatggga ttcctaacta tacagaacta accaaggcgg caggtaactt gaatgccgtt
    32581 gcaggcggtt ctagtgatac attcaaatcc gttgccatga tgctaacgca gacggcagga
    32641 gctgggaaac taacaactga aaactggaat caattagcag atgcgatacc gggtgcttca
    32701 ggattgttac aagacgctat gttgaaaaac ggagcttata caggaaactt ccgtgatgca
    32761 atggcgcaag gtcaaatcac ttccgacgag ttcaaccaag caattgtaca gttaggtatg
    32821 aatgacggag cagttaaggc agccacttct acagacacat tgagcggttc ttgggagcag
    32881 atgaaatcca ctgtaataaa tgggctacaa agtattatag aaaaaatagg cgttgaaaat
    32941 atcactggtt tcatcaacag agtaacaaaa gggattgaaa attctattcc taaaattact
    33001 caatttatag gttggttgag agatattgga acgtggatcg ttgaaaatag agagccacta
    33061 acatggattg tcggaatcat aggcggaatt acattagcag taaaagcatt gaacgtagca
    33121 agtatgttgc tggcaattac tggcggaaca ttggcagccc cttttgtggc gattggtgta
    33181 gcattaggcg cactagcagg tgctttggtg atagcttata caaaatctga aacatttaga
    33241 aatatagtca atgcggcttt tacagctgtg aaaaacgtag ttatgagcgt tatcaataat
    33301 ttggtggaat actacaaaat gttgtggagc gtgttgcagt ggctttggga aaaaataaaa
    33361 gaatgggctt catggattgg caataaattc attgaaatga agaacagcgt tgtgaatacg
    33421 gttcagaacc tatggaatgg cgtgaaaaac ttcttcagca atggcgttgg agatacgtgg
    33481 aataaggtag tcggttgggt aaaaaacatt ttcaacaaag caactgaatt gaagaacaac
    33541 gtttctgatg taatcggtaa cctgtggaat ggtatcaaag acacattccg tagaggtatc
    33601 gatacggtat tcaattggtt ttcagaacta ccaacgaaga tgaagaatgc cattattggc
    33661 ggtaaaaacg ccattgttga tgcgttcaaa agtattttca acgcagcact taaagcgata
    33721 ggtaaaccag ttaacgcaat catccatgga gcttcatggg tactagaaaa attgggcgca
    33781 gaacctctac aagaatggga tgtaccacaa tacgctacag gtacaccagc aggtgggcac
    33841 ccaatcaatg gtccaatgat ggttaatgat ggacgtggag cagaaacagt tatcacacca
    33901 gatggtagag ccttcattcc taaaggacgc aatgttgttc taaacgcacc aaaaggaacg
    33961 catgtcttga cagcagaaga gaccgcgcaa cttcaaggtt ctaaagctcc taagtatcgt
    34021 tacaaaaaag gtactaactt ctttggtaac atgtgggata gtgtgaaaaa ggttgctggt
    34081 aatgtaggtc acacaattaa aaacgtagta ggtgacgtgt gggactttat ttcagacccg
    34141 ggagcgttag ctagaaaagt acttgggggg ttagatgtat taggtggatt gacaaagtat
    34201 ccattagaag taggtaaagg catcctatct aaagcaacaa gtgcactgac tgaaaaaatc
    34261 actgggttgt tctcatctgg taacttagat acctccgtag gaacaaatgg cgtctataaa
    34321 tatttggctg atgttgctaa gtctgtgatg aagaaattcc caggatttgt ggcaactagt
    34381 gggtatagac caggtgaccc ttattcacat ggtaaacgta atgccattga tattgcatta
    34441 ccaggcgtca caggaggctc acctagatac acagaagcag caaactacgc ttttgacaaa
    34501 ttcgcttcca aaattggtta cgtaatcact aatgggaaag ttcgtgaccg ttcaggacaa
    34561 tcaggtcaac cagcaactgg tgcatgggag ccatggcccg atggagatca ctatgatcat
    34621 gtgcatttaa acggtgtgaa agacccacaa aacactcaaa tttcaggaga tagcgtggga
    34681 ggcagtgggg tagaaagatg gcgcaatgta gcaattagag cgttgaaaat gaccggtcaa
    34741 tacagtactg caaacttaaa tgcattacta aatcaaatgc gtacagagtc aaatggtaat
    34801 cctaatgcag ttaacaattg ggatattaac gccaaaaatg gaacaccatc aaaagggttg
    34861 ctccaagtga ttgacccaac attcagacag tatgcaatgc caggattcaa cagcaatatt
    34921 tttgacccac tatctaacat cttagcttca atcagatacg cactatcaag atatggctca
    34981 ctaacaaatg cctatcgtgg agttggttac gcaaacggtg gaattgtaaa ccaacatcaa
    35041 attgcggaaa tcgcagaagg aaacaagcca gaaattatta ttccgttaga taaggctaaa
    35101 cgatcaagag cgatgcaatt attgtctatc gcaatggaca aactaggagt ttctcctaaa
    35161 acttatggaa atgcgacaac tgtttccagt gattacgaaa tcctagaagc cattgaaaaa
    35221 caggcggcaa caacgaatca gctgttatcg ttattgctgg catttttcaa aggaaatagc
    35281 cgaactgata gagatttagc tttagatatt cagaagatct tggttaggag gatgtagatg
    35341 cgaactgtat tactgagaaa tagaataaac gaagaaattg atttgtccac agaagactat
    35401 tttgctactg ggttgagtaa tatggggttt gaagtaaaaa aagaacatgt gggacagtgg
    35461 gggaatttca gagaaagcag tgagagcgtt gaaatatctg aatttcagtc atctgtaatc
    35521 atttctgtgc atgggtttcg agaaaaagaa ctgtacaatt cacttgtgca gttcctatcg
    35581 gaaggtccgt ttgaactgga atttgctttt gacggcgaaa caatggtaag aagatgcagt
    35641 ctaaaatctt taagtaagac tgaaatcgat ccgaaaacat cgttactaac agacacttta
    35701 gaactatatt tcacatcaaa ctggtattcc gtaaaacgcg aaaaattaat tcagcgaccg
    35761 aatgtagtga aaacacgtgg taaggttttt ccgtataaaa gatcttatat ctacactcaa
    35821 aacttgtggg agaaaaaagg tgtattcaaa ttcaataata attctgtata cctaacgaat
    35881 agcaaggaac ggatgtcccc gctaaaaatt cgtgtgattg ggaagtgttc aaatccgtat
    35941 tgggaagtaa tccaaaactc acaaatcatc gcaacggatg gatacttcat agatatgact
    36001 gaaacacaga ctctagaagt atcaagcctt tttgaagata caacggcgat tttaaaagat
    36061 attgcagggg tagaatcttc tgtctaccaa caacaggatt atacaaaaac taattttgtt
    36121 caggccccaa ctggagaatt cagcattgtg tttcatgttg ggggggcaga cgtagagatt
    36181 gagttatacg aggagcgtga tctgttttga ttttagcagt cacgcttttt catcgtgatt
    36241 taatgcttta taacgaacat ttattctcta acagttttga atttggtgtg gacgagatca
    36301 acgaagaggc tagtagcttt acgatggata agtacgttcc tgtaaaaaca ggcgattttc
    36361 tactagcaaa atatattcct agtggaaaat ttgcatattt tggtgtaatt acgtcgcagg
    36421 aagacgaaaa aatcagttgt aaaagcttac ttagtttagc tgatagtgag ataccgactg
    36481 cacgtgtgtc aggagataac tacgaagagc atatccggcg gttgattgaa tactatttgc
    36541 tgaatgatcc aacgaaacaa ctaaaagata ttttagacgt caaagcagaa agtgcgacct
    36601 ctcattcgta tcaagctacc gatacgaata aacacaagtt aagtgcgtat attctcaatg
    36661 gtttcaaaaa atacaacgta aaatggtatt tcaagggaat tcaaaacaga aaaatttata
    36721 caggcatacg tgctgtaaat gaatcaattt acattaaaga caattcttct gaatttagcg
    36781 attgggatgt gtttgttcag gcgccgggcg ctggaaacga gaacaagcta ttaatcgttg
    36841 ataaagcaat gaaagatata gagaaaccga taatactgtc aacatggtat ttggacgaag
    36901 aaaacaattt gacacaagat ggatcgaaag aaaatatcac gaaaccaact gtcaatttgg
    36961 ttaatatcta cgatcaaacc gcagaagata aagcatccta cgaagatgtg gcaaaatcag
    37021 agttgaaagg caatacgtat tcgcatgaaa tcaaagtgaa cgttgtaaga aatgcaaaaa
    37081 atttgaatgt cgaaacgatt gaaacaggga tgtttgccac gatttcttat aaaggaaaga
    37141 tatacaagtc ggttttgaca gcttggcgaa tatcaagtga taaggagttt gtggaattga
    37201 ctttcggaaa cataagaagt cgttttatgg attattttga agataatggg ggataaaaaa
    37261 tggttagcaa tgtggatgga tatcaatttg aaaacgtgaa agtaagcgca gaaaatgatg
    37321 ctagacttta tcacgtttta tataatcgga aaaatcaggt tattgatggt tacgatcagt
    37381 ctatgaattt atcttcaagc ggattaacag taaaagttgc tgcaggagca gcgattattc
    37441 aaggtcgtat ggtcgttgtt cgacaagaag aaagtataac agttccagca aactcaagtg
    37501 gttatatagc attaacagtg gatttgacac aagaagttat acctggatct attcttccag
    37561 aatcggaaga atatgaatgg actaataatc aagtcaagct agaatttata acaaaagtta
    37621 taaaaggtaa tttaaacaat ggtgataagg tctataattt accactatgc tcagttaatt
    37681 ctactggatc aactgtttca atctcaaaga tatcggatag ttacgagctg actctctcta
    37741 aaggagaaat tttgtggagg gggactgcgt taatgcatga tactcaaact gtccaacctt
    37801 caaaaaaaat ttggcagaca gtttcaggtt ttttgttagt atggctccca tacgaaaacg
    37861 ggcaagcaat tgaggataga tatgtaacta cgccttttta taaggagcgt gtaactttta
    37921 ctaatgtttt aggagaaatt gtttcaggat ttgatgacta tcacaaaaag tggtttagta
    37981 aacgaataaa ctataattcg aatactaata tatttacagg tgcagcaagc aatgcaagtg
    38041 gagataacgc aaatatggtg cttaggtata tagtttcttt ttagttagga ggtatggaag
    38101 agtggcaaat ttagaaatta aattatctgc aaataagaga cagccttatc tacgtcaccg
    38161 tgttgttggt agagttggtg atggggggct cacaacaatc aatgtacaac ttcttgagga
    38221 agatgaaatt acaccttttg taattaatct aaacggtact ttgaaatttg tgggcgaagt
    38281 ttcaaacggt aactataccg agggagaacc agaaataatc gattcgacta atgggttaat
    38341 tagttacacg ttcactaagt caaatttcag cacgagtcat caattcaaac aagcatattt
    38401 tgaatatgta gatcctaacg gcaaaaaagt aacttttcag aacttcatca tagacgtgtt
    38461 agaacgagtg gatattaatt cggagcaagc gaaatactat atttcttcat tggaaaaatt
    38521 acagagtgaa atgcaaacca ctttcaatca gttcattagt gataaacagg tccaatacga
    38581 tcaaatctac tcgaaatata acgaattagt aagattgata aatgaatcag ataagcaagt
    38641 aaatgatcgt attgatcaaa ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat
    38701 gtacagtaac agcatcgact ttgggggcta tgattattcg gggaatccga atttgttaag
    38761 taagctatca tacgacttaa ttgaaaatca aaatacttca gctggaacac tttctaaggg
    38821 tgaaaactcg tttaaatata ataagatatc agctgaagtg gaaggtggag tagagttata
    38881 ttataaacga agaggtatag ctaactggtt accctctaat aaaacgcttg taatgactgt
    38941 taagcttaga gctggagcgg actatagtcc agttgacgga aaacttatac tgattagata
    39001 taggtatgtt gacagtggaa ctggcaagat tgttttagac ttacctatta acagtaattc
    39061 gataactcag gaatggaaag agtttagtat tactggaact actccaacat ttagcccaca
    39121 agcataccat ccttggatac aatttagggc tcaagatggg gtacttgggg aaatagaaat
    39181 gagctatgac atcaaaatcg aagaaggctc tacagctaca ccatatcagc ctaacttact
    39241 tgatgactct tactggctag gtaaaacacc gttgggtgag aatttgataa cgaatgccaa
    39301 gttcccaatt ataacacgaa gcaatcctat ttctggtttt gatatttcag aagaattaat
    39361 aataggtgaa acttatacgg tatctttaaa agggactaag ccagctacta aagaattcca
    39421 cttatactac ggagaagcaa attaccagca gttgcagtat caggcaactt tacttcctgt
    39481 ggaaggatta acagatgtgt ggagtgccac ttttacagcc agaaatacta gtgaaacgac
    39541 aaatttactt agagtagcgt tatggcaaaa acctaattct gcaacatatg atactgttca
    39601 aattgactgg ctcaagattg aaaaaggcga cacacgcaca ccaaatatca gtcagtttaa
    39661 atactttggt gaaggcttga aagacagtaa caatccgaac gactacagct gggatgtcac
    39721 accagagtat actgaaaaaa gcttgaataa tacggttagt ctgactgaac cgcaatccat
    39781 cgaaggattg aaaaatttcg aagatggtat tcaatcaaaa ggaaaatctg tattgacatc
    39841 agacgacaac aaatatgaag tcgtaacctt aacagttaca aacgggaata ccggatcagc
    39901 aaagctttat cgtgaaggaa aaaccgtcac tatttatttt tttgcgttga atgggaaaag
    39961 cagtggtgga aatgattcaa cgatactaac aattccagaa ggctatcggc caccaattag
    40021 ttttgagcaa ctggttggct cgatagaccg ttctactttg aacagtgctc agttatctat
    40081 tggtgcagat ggagccatta aatggcgaag aaactcaagt tatggatcgg attatacctt
    40141 tgcaattact tacacgattt agaaaggcgt gaatcgatat gaaggcagca tatagaccaa
    40201 ttgaacctta cggattcgag caaatcattg tgaatgatga agaacattta ccggaagaat
    40261 gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa attcaattac tgggagggaa
    40321 ataaatgaaa aacatttgga aatatggccg tactggcgga gagtatgcag gaaaagtatt
    40381 ggacgacatg cttgtatccg ttccttacac agatcagcca ccgctcgaag gggttcgttc
    40441 agatggcgaa ccgctaacga ttgctgatca gatgtttgat cctaaattga accaatggat
    40501 tattttagcg aacgcgttag atcacaacga tttaaacaat ctcaaagcga tgtacgaggc
    40561 tcttgaacat gaaaacggca acctaaaaca gctaaatgcc aaactcatgc taagcgatgt
    40621 agcgattaaa caggaaaata ctgcattgaa agaaaaagct gacagtttag cacaaatcaa
    40681 ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac atttcagaaa ttaaagagca
    40741 actaaatcca gcttcaaagg gaggtgagta gtatgtttag ttttagcgat gtgaaaatga
    40801 tgtatgattg gggctgtttt actgacgatc aagttcgtct attcgttcca ctatgtatca
    40861 cagatgaaga agcagataga attattagca aagaagagag cgcatcttaa gtgatgcgtt
    40921 tttttgttgg aaagttggtg gaacatgaaa gaagaagcgc tccaagacgt tgtggagaga
    40981 ttagtaagaa ttgaaacaaa attagacaac tacgaatcac ttagagaaaa agctgatagt
    41041 gcaaaagatt tggcagacaa agcctactca gtagcgctaa acaacgcaga agatatcaaa
    41101 gaaatgaaag ccaataataa atggtcgtgg ggttacatga tcggtttagg cattacgatc
    41161 attggctatt tcttgactaa attgtaaagg agttaagaag aaatgatttt acccgataag
    41221 tattatcaag tcattaaatg gacggtttta acagtattac cagctgcatc tgttttagta
    41281 gccacgttag gaaaagcgta tggatggaat ggaacagata tgacagtact cactatcaat
    41341 gcagtagcaa catttttagg cgttatcact ggtgtgtctg catataactt aaaaaaatag
    41401 gaggaaaaaa atgaagaaga aaatcatttt atcattgagc ctaataatgg ctcttttttt
    41461 attgccaatt aacgggttcg cctatacgat caacaatgaa tttaatttag gtgtaaatga
    41521 aggtagctca caagtagcaa ataaccaata catcctattg catgaaacag caaacgaaac
    41581 tgcgacagga cgaaatgaag cgcagtacat gaaacgttct tggtacaatg cctatacagc
    41641 gtatattgtt ggcgatggtg ggatcgttta ccaagttgga caacctggtt atgtacaata
    41701 tggcgctggt tcatatgcta atgcaaatag tcctgtgcaa atcgaactgc aacatacgca
    41761 cgataaagta acgtttgaaa agaactacaa ggcatatgtt gaattagcga gagattccgc
    41821 taataaatac ggtattccac ttacattaga cacaccgtat aatcaaccag gaatcaaatc
    41881 acatttatgg gtaactcaaa acatttgggg cgatcacact gatccgtatg gatacttatc
    41941 agaaatgggt gtaagtaagg aaaaactggc ttacgattta gctcatggat ttacagacga
    42001 aaacccaacg acttctgaga acaagcctgt cattgatcca acccgagcag gtgcagcaaa
    42061 tccaacttta tcagatggaa cgaaccattc tcacattgat cagtttggag aaatcgaaaa
    42121 tgcgaactta cacgtcgctg gatggcacat cgctaactat aaatacgagt atattttcat
    42181 tatggactac aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc
    42241 ggatgtaaat caagcttata atacttctgg aaacgttggc tatcatgtat cttttaacat
    42301 gcgcaatttc cctaataaga aagtatacgt catgatgcgg gcaacgaatg atccagaagg
    42361 aaacactaaa ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa
    42421 acgataaaaa tagctcctcg ttgaggagca gtacatagaa ttgaaaatta acgttaaatc
    42481 aaaaaaatat ttacaagatg tttatttaag tagataataa aagaaaaaag caggtgaaat
    42541 tgtgacagca gaaattggta ttatgaataa aagtggaatc gtgttagctt cagattcagc
    42601 atctacgatt ggagatagca aagtatataa tactgcaaaa aaattattta ctttggattc
    42661 catgcattct gtaggtatca tgatatatgg taatgctgaa tttaatggta ttccttggga
    42721 aataatcatt actcaatata agaaaagcat tggtagttct gtttttaata ctttggaaga
    42781 atacgcggat aattttatag aatttgtcaa aacagcatct tttattagaa gtgaacagac
    42841 cgagcaagaa caaatgattg gggtttttca aaaaataatt tcaggtttat ttgagagtat
    42901 agaagtagat attaactttt taataagcca aggtacacaa atagacaaag atgtcctagt
    42961 gaagttatta caaacaaaaa tgaataccaa tttgtctcaa cagtcccaga cattcatttt
    43021 agatattgaa aaggcgttat ttcttagtaa ctatggagaa atattaaaag atattcttaa
    43081 tagtatttct acaatggaag gtgtttcaga agctatatca gaggaaattc aaagttacgt
    43141 ttatgaaatt ataattagag acgatgttta ttcttcccct acaggaatag ttatagcagg
    43201 atatggaaga atggatattt tccctaaact atattcttat aatatgtttg gatttgttat
    43261 gaatattctg aaatactcag aatatgaaag tgctcaaata ggaaatgaca atggttcatt
    43321 aagatctact atattaccat ttgctcagtc tgatgtggta aatactgtag ttcaaggggt
    43381 agatccacaa attactaatt atctgtcgag tcaagtcgat agttttgacg ataacggaaa
    43441 aaatacctat attaatatta taaagaatat ttcagagttt caacaaaatc aattcatatt
    43501 tccgttacta aacatgattg ctttactccc agttgaagaa acagcaatta tcgcagagac
    43561 tttattaaat ttaaccagtt ttaaacgaaa atatactact tctgtagaaa cagtaggtgg
    43621 cccaattgat gttttagcta ttacacctaa tgatggccct atttggatta aaagaaaaca
    43681 ttattttgat attgataata atattggata cagattgcga aaggagagag cgaatgatta
    43741 caataattaa acctcaggaa aaatcccctg ttttgaaatt taatgagagc aatctagccc
    43801 ataataaaat gagtataact gatacagtat ttgatgtaat ggttgagcaa caaaaaaaaa
    43861 ttaataataa caatagacaa agaaaagaaa ttaaccaata ttaatgtttt ttctgttgcg
    43921 ttttttgcag tcataattta aaacctcgct caatcttggg cgaggtcttt ttttattttt
    43981 ctgaaaagtt aaagtaaata atagcataaa ataatatatt ttacaaagaa taagtacaat
    44041 ctagtttttt gctattaaat gtgtaataat aaatatgcca tcacaacatg aagaatgaaa
    44101 tccattatta tctagtctag tccattcttt ttgtttgcag tatttgtgat ggctttc
//
LOCUS       DAZKPO010000014        39410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000014 DAZKPO010000000
VERSION     DAZKPO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.014"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8NZ_000772"
     CDS             <1..878
                     /locus_tag="ACG8NZ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0538469.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="ACG8NZ_000773"
     CDS             complement(1119..2735)
                     /locus_tag="ACG8NZ_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0538470.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="ACG8NZ_000774"
     CDS             2853..3071
                     /locus_tag="ACG8NZ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538471.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="ACG8NZ_000775"
     CDS             3266..3727
                     /locus_tag="ACG8NZ_000775"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0538472.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="ACG8NZ_000776"
     CDS             complement(3920..4159)
                     /locus_tag="ACG8NZ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538473.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="ACG8NZ_000777"
     CDS             complement(4183..5706)
                     /locus_tag="ACG8NZ_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0538474.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="ACG8NZ_000778"
     CDS             complement(5724..5846)
                     /locus_tag="ACG8NZ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0538475.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="ACG8NZ_000779"
     CDS             complement(5876..6859)
                     /locus_tag="ACG8NZ_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0538476.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8NZ_000780"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8NZ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0538477.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="ACG8NZ_000781"
     CDS             complement(7053..7430)
                     /locus_tag="ACG8NZ_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538478.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8NZ_000782"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8NZ_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0538479.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8NZ_000783"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8NZ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0538480.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10214)
                     /locus_tag="ACG8NZ_000784"
     CDS             complement(8448..10214)
                     /locus_tag="ACG8NZ_000784"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538481.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="ACG8NZ_000785"
     CDS             complement(10174..11928)
                     /locus_tag="ACG8NZ_000785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538482.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..12607)
                     /locus_tag="ACG8NZ_000786"
     CDS             complement(12038..12607)
                     /locus_tag="ACG8NZ_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0538483.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12625..14040)
                     /locus_tag="ACG8NZ_000787"
     CDS             complement(12625..14040)
                     /locus_tag="ACG8NZ_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538484.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(14051..14719)
                     /locus_tag="ACG8NZ_000788"
     CDS             complement(14051..14719)
                     /locus_tag="ACG8NZ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0538485.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14849..15433)
                     /locus_tag="ACG8NZ_000789"
     CDS             complement(14849..15433)
                     /locus_tag="ACG8NZ_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0538486.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15764..15997)
                     /locus_tag="ACG8NZ_000790"
     CDS             complement(15764..15997)
                     /locus_tag="ACG8NZ_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0538487.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(16012..16962)
                     /locus_tag="ACG8NZ_000791"
     CDS             complement(16012..16962)
                     /locus_tag="ACG8NZ_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0538488.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16959..17801)
                     /locus_tag="ACG8NZ_000792"
     CDS             complement(16959..17801)
                     /locus_tag="ACG8NZ_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0538489.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17803..18522)
                     /locus_tag="ACG8NZ_000793"
     CDS             complement(17803..18522)
                     /locus_tag="ACG8NZ_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538490.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            19070..19360
                     /locus_tag="ACG8NZ_000794"
     CDS             19070..19360
                     /locus_tag="ACG8NZ_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538491.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            19432..20658
                     /locus_tag="ACG8NZ_000795"
     CDS             19432..20658
                     /locus_tag="ACG8NZ_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0538492.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG8NZ_000796"
     CDS             complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG8NZ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0538493.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG8NZ_000797"
     CDS             complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG8NZ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0538494.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(22116..22583)
                     /locus_tag="ACG8NZ_000798"
     CDS             complement(22116..22583)
                     /locus_tag="ACG8NZ_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0538495.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(22703..24241)
                     /locus_tag="ACG8NZ_000799"
     CDS             complement(22703..24241)
                     /locus_tag="ACG8NZ_000799"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0538496.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24321..25007
                     /locus_tag="ACG8NZ_000800"
     CDS             24321..25007
                     /locus_tag="ACG8NZ_000800"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0538497.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG8NZ_000801"
     CDS             complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG8NZ_000801"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0538498.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG8NZ_000802"
     CDS             complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG8NZ_000802"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0538499.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(26493..27623)
                     /locus_tag="ACG8NZ_000803"
     CDS             complement(26493..27623)
                     /locus_tag="ACG8NZ_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0538500.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(27623..29806)
                     /locus_tag="ACG8NZ_000804"
     CDS             complement(27623..29806)
                     /locus_tag="ACG8NZ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0538501.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(29803..30828)
                     /locus_tag="ACG8NZ_000805"
     CDS             complement(29803..30828)
                     /locus_tag="ACG8NZ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0538502.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(30873..31700)
                     /locus_tag="ACG8NZ_000806"
     CDS             complement(30873..31700)
                     /locus_tag="ACG8NZ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0538503.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(31697..32764)
                     /locus_tag="ACG8NZ_000807"
     CDS             complement(31697..32764)
                     /locus_tag="ACG8NZ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0538504.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(32790..33626)
                     /locus_tag="ACG8NZ_000808"
     CDS             complement(32790..33626)
                     /locus_tag="ACG8NZ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0538505.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(33623..34492)
                     /locus_tag="ACG8NZ_000809"
     CDS             complement(33623..34492)
                     /locus_tag="ACG8NZ_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0538506.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(34562..35845)
                     /locus_tag="ACG8NZ_000810"
     CDS             complement(34562..35845)
                     /locus_tag="ACG8NZ_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0538507.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(35859..37544)
                     /locus_tag="ACG8NZ_000811"
     CDS             complement(35859..37544)
                     /locus_tag="ACG8NZ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0538508.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            37819..38844
                     /locus_tag="ACG8NZ_000812"
     CDS             37819..38844
                     /locus_tag="ACG8NZ_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0538509.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT        12021 a         7872 c         6346 g        13171 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    12541 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    12601 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    12661 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    12721 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    12781 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    12841 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    12901 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    12961 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    13021 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    13081 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    13141 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    13201 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    13261 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    13321 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    13381 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    13441 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    13501 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    13561 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    13621 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    13681 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    13741 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    13801 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    13861 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    13921 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    13981 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    14041 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    14101 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    14161 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    14221 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    14281 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    14341 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    14401 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    14461 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    14521 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    14581 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    14641 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    14701 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    14761 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    14821 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    14881 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    14941 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    15001 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    15061 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    15121 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    15181 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
    15241 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
    15301 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
    15361 cggtcaggca cagttgcctt atcttagttt gctgaaatcc ttctttttca aaatggctta
    15421 gtccactttc tattaacttt tggtgagtta taatataatc tctggtttct atgggtactt
    15481 cctcctaata agatgaatta actttattat cttacacagg acttgaagtt tcaatcgtta
    15541 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
    15601 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
    15661 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
    15721 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
    15781 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
    15841 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
    15901 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
    15961 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
    16021 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
    16081 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
    16141 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
    16201 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
    16261 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
    16321 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
    16381 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
    16441 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
    16501 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
    16561 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
    16621 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
    16681 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
    16741 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
    16801 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
    16861 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
    16921 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
    16981 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
    17041 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
    17101 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
    17161 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
    17221 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
    17281 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
    17341 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
    17401 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
    17461 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
    17521 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
    17581 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
    17641 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
    17701 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
    17761 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
    17821 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
    17881 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
    17941 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
    18001 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
    18061 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
    18121 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
    18181 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
    18241 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
    18301 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
    18361 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
    18421 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
    18481 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
    18541 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
    18601 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
    18661 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
    18721 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
    18781 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
    18841 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
    18901 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
    18961 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
    19021 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
    19081 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
    19141 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
    19201 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
    19261 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
    19321 ctttgacgta gaagatatta aagactttat gaatacttga aattttcttc tcgccgcata
    19381 tactaaaatt agtgaaatat actgccttac tttttttaag gagtgaggat tttgcctaca
    19441 aagctttcaa atgggaaata taaaactaat ctgagatatc caaaaaggtt taaagaaata
    19501 acaggaattg catcagaaaa gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa
    19561 gccgaaaatg atatgaagaa gaaaatagaa aaagttttga gagaggagaa tgcaaactca
    19621 ttagaattaa aaggaaaaat aacgtttaaa aaattttacg aatctaaatg gcttcctaga
    19681 tatgaattag gtcaaacaat tagatcaaac aggcctccta gtgatataac cattagtaat
    19741 acaaaagata tatttcggtt gcatatttta cctatgtttg gtgaatacgc aatgaattat
    19801 ctaaatatca atactgagat aatatcagat gaattaacca aaaaatcgaa agaatatgca
    19861 aatataaaaa tcataaaagg atatgttcgt tccatgttcg atattgcaga aatactaaat
    19921 tatatcgaat ttaatcgaac tactaaaatt attcaaagta tcactgcacc taaaaaaaat
    19981 gctttagaag aaaaaagaat acaagaaggt aagcaagcac tatcatcaaa agaacttact
    20041 aattggatag aagcagtaaa tgatgatttt aataatcatt tacttacttt tcatgattac
    20101 acacttttta tgattaccct gtatcttggt gatagaaaaa gtgagactta cgctttgcaa
    20161 tggaaatata ttgattttga aaaacaaacg gtccgtctta aacatacttt agataaatat
    20221 caaagaaaaa agtttacaaa aggtcgaaaa gataccgtca ttcaagtacc agaagttgta
    20281 atgacattgc taagtgaatg gaaatcggta caagctgatc aattgttgaa attaaaaatt
    20341 aagcaaacgt tagatcagta tctctttact tatactaaac catctggaga agtaaattgt
    20401 cctgttcatg ccgattacct aaactatcga atcaattcaa ttaaaagaag gcatcctgac
    20461 ttagttcatt tatctccaca caaactccgt catacttatg caactatagc tagacaaggt
    20521 ggagcagata tgaatcaaat ttcaaatgct ttaacacact cagatatttc tacaacaaag
    20581 atatatgtga acacaccaga tatagttgat aaggctgttt ttgaagcttt tcagagagga
    20641 ttgaataagt gtgattgata tgtgttctta aatgtgttct taaaaacaaa agaagcactt
    20701 tccaaataat tgaaaagtgc cagaaacgtt gatatataaa ggatattaac gttttgagaa
    20761 ttgtgaagct ttacgtgctt tcttaagacc tggtttttta cgttcgacca tacgtgcgtc
    20821 acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa
    20881 tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac
    20941 gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac
    21001 ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt
    21061 gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat
    21121 atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac
    21181 ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa
    21241 ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg
    21301 acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt
    21361 gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca ctttgtctgc
    21421 attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt
    21481 accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc
    21541 tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt
    21601 attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg
    21661 tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc
    21721 tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt
    21781 cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct
    21841 tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa
    21901 aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggctta
    21961 caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    22021 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatttc
    22081 acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc tggcgctttg
    22141 cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag
    22201 atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt ttggtaaaga
    22261 tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat agtacgatcc
    22321 cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa tgtagaataa
    22381 ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc gttcaaatca
    22441 tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa
    22501 tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg atttaatact
    22561 tctttggttt gttggaattt catctttctt gcctcctaac attttcttac acttaaaata
    22621 atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga ttttcagttc
    22681 gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct cgcagttgag
    22741 caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat ggatagattt
    22801 tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat tctatcaatt
    22861 cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa atcacagctc
    22921 ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt acatcagcca
    22981 tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc tctgtgagct
    23041 gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc ccttctagaa
    23101 tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg
    23161 catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa atcagaccat
    23221 tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga ttttctcctt
    23281 tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc
    23341 cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga acataacaaa
    23401 acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt gctccgctcg
    23461 ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga ttagaaagcg
    23521 gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt tctgctgctg
    23581 tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc aattgctcta
    23641 ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg atatcaaaat
    23701 atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga
    23761 tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt tggctgtttt
    23821 gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca gtctgccgat
    23881 aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct gctcggttat
    23941 ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat atcaggccat
    24001 gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta
    24061 cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt tctgccatcc
    24121 cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct tgggcttcat
    24181 ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt gaaacgttca
    24241 ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt
    24301 tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt atcggatcat
    24361 ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat cctagatctc
    24421 attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt ctctcgatca
    24481 tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac tatttgttgg
    24541 cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc gcaagaaatt
    24601 tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat ttattttact
    24661 tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt ttttggttgc
    24721 aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc
    24781 ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt tcttggagtg
    24841 gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg tttggaatat
    24901 ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg cctttcattc
    24961 cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga
    25021 caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct tgtgacttgg
    25081 agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct gaccctcagt
    25141 attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa gtagcttttt
    25201 cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt
    25261 ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg taatcttctg
    25321 caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc
    25381 tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca tcattatagt
    25441 agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat
    25501 cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct ggatcatatt
    25561 cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca taagttggat
    25621 ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc tgctcttcaa
    25681 atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa
    25741 atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt ttgcttgtga
    25801 cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc aaaacgaacg
    25861 tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa gatttatgtt
    25921 ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac cagcttctga
    25981 atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc ctttgagaaa
    26041 aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga catcaaaata
    26101 acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc
    26161 gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag gcaaaacgga
    26221 tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct cagcaaaggc
    26281 tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca atcgctctag
    26341 tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga atggaatgga
    26401 cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt ctacaagaac
    26461 gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg aaaatgaacg
    26521 acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt ggcttgctgt
    26581 aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga aatgccaaca
    26641 tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac aggaacgccc
    26701 tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg tccatctaga
    26761 cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa
    26821 tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca
    26881 cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata
    26941 taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt
    27001 ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg
    27061 acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt
    27121 ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa
    27181 cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg
    27241 atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg
    27301 gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc
    27361 atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat
    27421 gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc
    27481 gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct
    27541 tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa
    27601 tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca
    27661 gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta
    27721 ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag
    27781 aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg
    27841 taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa
    27901 ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata
    27961 aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac
    28021 tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg
    28081 gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga
    28141 cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac
    28201 gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa
    28261 tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt
    28321 tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca
    28381 gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc
    28441 tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt
    28501 catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat
    28561 actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa
    28621 cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac
    28681 cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt
    28741 gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa
    28801 actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat
    28861 aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca
    28921 taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt
    28981 ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa
    29041 tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt
    29101 gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga
    29161 tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat
    29221 tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga
    29281 tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg
    29341 tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga
    29401 tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga
    29461 cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt
    29521 ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac
    29581 cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg
    29641 ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt
    29701 cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat
    29761 acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc
    29821 tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca
    29881 gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact
    29941 tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac
    30001 atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac
    30061 attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc
    30121 gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca
    30181 tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac
    30241 gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata
    30301 atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac
    30361 accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg
    30421 attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg
    30481 cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg
    30541 tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa
    30601 gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac
    30661 tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac
    30721 tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg
    30781 tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt atttctccct
    30841 cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga
    30901 cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca
    30961 ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc
    31021 gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct
    31081 gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct
    31141 tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag
    31201 tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa
    31261 ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat
    31321 tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga
    31381 taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt
    31441 gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca
    31501 ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt
    31561 gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca
    31621 aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga
    31681 tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt
    31741 cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa
    31801 ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt
    31861 cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag
    31921 caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa
    31981 aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct cttaaagaag
    32041 cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca cgtttatctg
    32101 tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg taatcggcac
    32161 cgtatttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg atggtagtag
    32221 cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg
    32281 ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg aattgagcag
    32341 gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt agtttataat
    32401 tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag actaactctc
    32461 ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca atcatatttc
    32521 ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt ggcaagaata
    32581 ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta aagtcgacaa
    32641 tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg ttcttttcga
    32701 tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca actaattttt
    32761 tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc gactaaatca
    32821 cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc ggtcaccaat
    32881 gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg
    32941 attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa ataatcattc
    33001 cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc caatggtata
    33061 atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc ttcttcaata
    33121 tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg aagtgtttga
    33181 acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc ggaaatgatt
    33241 ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta aaagctagca
    33301 ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc gctgatccca
    33361 atcactactg cagttacgga ggctatcaag gagatcacta aactattttt gaaatacatg
    33421 atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt ccattctttc
    33481 ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc
    33541 atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc gtaaaataag
    33601 attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag agcaagacga
    33661 ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca agatcattga
    33721 acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc gtcagtagat
    33781 agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca gttgctagta
    33841 ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt gcaccgtcca
    33901 tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa
    33961 acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag ctagcaactg
    34021 gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact aacagatcat
    34081 tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa gaaatagatg
    34141 gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg aacttgcggt
    34201 taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact aagaatgtgt
    34261 aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt tcaagataat
    34321 tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg aaaaaactga
    34381 tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc atgcttggtg
    34441 ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc attgaaacct
    34501 ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc agaacttctt
    34561 gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg tttccgacag
    34621 ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc attttcgtga
    34681 aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta gcaatctcag
    34741 atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc agttcgacta
    34801 cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt tccatgtttt
    34861 tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc tcgtctagac
    34921 cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt tgtgttggga
    34981 tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa atgtaagttg
    35041 aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct gtcacatcat
    35101 tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct tctctcatct
    35161 tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca tttgacaaag
    35221 caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata gcaattccat
    35281 attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca tcccaatttt
    35341 ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata ccttgaaccc
    35401 agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca aggtttttgg
    35461 ctccgtcata ataattatct tctccagctt cctcgataac gttttggact gccgtctgat
    35521 caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc attacttctg
    35581 gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat tcttctaccg
    35641 gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc agttcattga
    35701 tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg
    35761 attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta gctagcagac
    35821 aggtagtaac aataattttt ttcatttttt tccacctctt attttattat tttcagccac
    35881 atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat aatttgtcca
    35941 gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg attggaaaaa
    36001 ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat aaaagaatct
    36061 gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt ttttcgaata
    36121 gatagcatct gggtcaattc accatatact gctgttctta atggatctgt ctgcaactct
    36181 ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc tcctgttttt
    36241 tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat tgccggcacg
    36301 cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct ccattccgtc
    36361 gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata tggactggtc
    36421 gttccatctg ggtttttctt gtagtttact cgggtaccct ctttcttcag ctgttccact
    36481 aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc gataccgtca
    36541 tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac
    36601 cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa aggtggtagc
    36661 gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact gatattatct
    36721 ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc atcagcgatt
    36781 gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga agttcctagt
    36841 tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt ttctaagtaa
    36901 aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt caagtctact
    36961 tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt
    37021 gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc cagtggcaaa
    37081 tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa ccaagtactt
    37141 tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct tctcatttct
    37201 tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac aacggagaaa
    37261 ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa tgtatctgcc
    37321 aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat
    37381 tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg
    37441 cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt tttctcagac
    37501 aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt cccctttctt
    37561 ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg tcaatagcat
    37621 ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat
    37681 taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca
    37741 aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa
    37801 aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc
    37861 tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa
    37921 gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga
    37981 ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc
    38041 tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat
    38101 aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg
    38161 aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa
    38221 acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg aaaagtcgga
    38281 aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg
    38341 gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa
    38401 gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta
    38461 gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca
    38521 atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta
    38581 aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt
    38641 tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg
    38701 atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc
    38761 caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat
    38821 tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa
    38881 gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta
    38941 gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt
    39001 taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt
    39061 tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt tggggatact
    39121 tatctctaaa acataaatag aataacagac atgtctactg gttttgtttt attcgaggaa
    39181 ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc atcaatttat
    39241 tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt ggaagatgat
    39301 cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc cacttttctt
    39361 ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata
//
LOCUS       DAZKPO010000015        37908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000015 DAZKPO010000000
VERSION     DAZKPO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.015"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8NZ_000813"
     CDS             133..3660
                     /locus_tag="ACG8NZ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0538510.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8NZ_000814"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8NZ_000814"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0538511.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8NZ_000815"
     CDS             7448..7789
                     /locus_tag="ACG8NZ_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0538512.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8NZ_000816"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8NZ_000816"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0538513.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8NZ_000817"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8NZ_000817"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0538514.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8NZ_000818"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8NZ_000818"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0538515.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8NZ_000819"
     CDS             13000..13737
                     /locus_tag="ACG8NZ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538516.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8NZ_000820"
     CDS             13751..14566
                     /locus_tag="ACG8NZ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0538517.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8NZ_000821"
     CDS             14579..15226
                     /locus_tag="ACG8NZ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0538518.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8NZ_000822"
     CDS             15242..15898
                     /locus_tag="ACG8NZ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0538519.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8NZ_000823"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8NZ_000823"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0538520.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8NZ_000824"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8NZ_000824"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0538521.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8NZ_000825"
     CDS             18240..18758
                     /locus_tag="ACG8NZ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0538522.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8NZ_000826"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8NZ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0538523.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8NZ_000827"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8NZ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0538524.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8NZ_000828"
     CDS             20691..21743
                     /locus_tag="ACG8NZ_000828"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0538525.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8NZ_000829"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8NZ_000829"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0538526.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8NZ_000830"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8NZ_000830"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0538527.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG8NZ_000831"
     CDS             complement(23383..24144)
                     /locus_tag="ACG8NZ_000831"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0538528.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACG8NZ_000832"
     CDS             24333..25427
                     /locus_tag="ACG8NZ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0538529.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..26472)
                     /locus_tag="ACG8NZ_000833"
     CDS             complement(25513..26472)
                     /locus_tag="ACG8NZ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0538530.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            27298..27477
                     /locus_tag="ACG8NZ_000834"
                     /pseudo
     CDS             27298..27477
                     /locus_tag="ACG8NZ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27656..28606)
                     /gene="yidC"
                     /locus_tag="ACG8NZ_000835"
     CDS             complement(27656..28606)
                     /gene="yidC"
                     /locus_tag="ACG8NZ_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0538531.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(29044..29319)
                     /locus_tag="ACG8NZ_000836"
     CDS             complement(29044..29319)
                     /locus_tag="ACG8NZ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0538532.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29427..30191
                     /locus_tag="ACG8NZ_000837"
     CDS             29427..30191
                     /locus_tag="ACG8NZ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0538533.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            30314..30817
                     /locus_tag="ACG8NZ_000838"
     CDS             30314..30817
                     /locus_tag="ACG8NZ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0538534.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            31225..31871
                     /locus_tag="ACG8NZ_000839"
                     /pseudo
     CDS             31225..31871
                     /locus_tag="ACG8NZ_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     regulatory      31913..31979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            32034..33302
                     /locus_tag="ACG8NZ_000840"
     CDS             32034..33302
                     /locus_tag="ACG8NZ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0538535.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(33329..34528)
                     /locus_tag="ACG8NZ_000841"
     CDS             complement(33329..34528)
                     /locus_tag="ACG8NZ_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0538536.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(34647..35954)
                     /locus_tag="ACG8NZ_000842"
     CDS             complement(34647..35954)
                     /locus_tag="ACG8NZ_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0538537.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            complement(36070..36192)
                     /locus_tag="ACG8NZ_000843"
     CDS             complement(36070..36192)
                     /locus_tag="ACG8NZ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538538.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            36425..37543
                     /locus_tag="ACG8NZ_000844"
     CDS             36425..37543
                     /locus_tag="ACG8NZ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538539.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT        12298 a         6613 c         7962 g        11035 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag
    25861 ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga
    25921 taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc
    25981 ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga
    26041 gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt
    26101 catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtgttctcat
    26161 accgcatgaa acaggccttt cttttcgccc gataatgacg tcaggcgtcc aaccaagagt
    26221 gactttctct ttaatgtaat ctacttcatg agcaggtaat tgaatgactt ttctaccaca
    26281 ttttttctta ttttctttat actggtgcca ataatcaaga gcagtcttgc cttgcttgaa
    26341 cttattgact acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac
    26401 cggcttatta tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg
    26461 tttgtaggtc atttatggtt acactccttt gttttctttc gtcggaaata caatttgagt
    26521 gtaccataaa tgccttttta tttttctaac ttaattttac aattcgcgtt atttagagaa
    26581 caaacttgtg taacatacaa aggaaatttt gaatatgaaa gatgaggaac taaaagaaga
    26641 tttaatagat aaattaggga ttgcactgag aatggttcga agagcagtcg caggaaatat
    26701 gtttacacaa tgagcattca acaaaaagaa tggtcttttt tcattgtcat ttttcaataa
    26761 agaagttttg ccagaggaaa tttttttaag ctggcatagt tgtccggtat gagattgaaa
    26821 aaatacagtt gtgtctgaca gctcatttga gaaattaata agataagttc atcaaaatag
    26881 attcagagcc cccttgatag agattaaaaa acagcaagag tgtctttaaa gcagctagga
    26941 aataaaaaag atggagagaa gccttatttt gctgattttt aaataccatc ctgagtaaaa
    27001 gtcagaaagg cggttgttat gcaaacattt agagatttga agaaatttgt agttgctaca
    27061 aagaaagtat aaaagaaacc aacatttaag aagttgcgga aaggagccag tttctatccc
    27121 aaaaaggtga ttcaaaacaa ttcggttaca atagcggttt ataagaaggg aaatgttgtg
    27181 tatcaagctt tgaatcgtgt aattgtattc ccagtagaaa aagggatgga tactatcact
    27241 attcttctac tagaaaggtt tatggatgtt atttagatgg gtggtatctc cgcgttgtcc
    27301 tttatggaga atggtgtctt atgtgggcga gcaacatggg cgaagggagt ggcgcctttt
    27361 gttaccgaag gcgaagaaaa agcgagtagt tggatgttag aagaaggaaa acaaaatatt
    27421 aaggaattaa gtacactatt agaagaaaca gcaacacctg tctacgttga tagataagaa
    27481 aaagttgttt cttcataaaa agatgctgct gcaattagat agcgatcaat cgatgattca
    27541 aattaaacat tcacttaaat aaaaatatcg accagatttc tttttcaaaa atcttggtcg
    27601 atatttttaa ttgctaaaac tgttaatgaa tttttattac actcttattt actatctact
    27661 tccgcttctg ctgctttcct gcgttgcggc ctttattgtt tgtactgttt ttgggttgct
    27721 tgatttgttt ggttgcttgt ttgctgtcag ctggtttagc tgattctgtc acatcaattc
    27781 ttggagtgac gacttgtttt ggtgggtttt gtttcaactc ttctgccact tgtgctttca
    27841 aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac acaccaccga
    27901 ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg atcatagcag
    27961 gagagacgat cagcatcgta cgcatcgtct ttttctgttc ttcaggaata ccgatcgttg
    28021 agatgtatcc ttgaagcaga taagcgaccc ctgcaatggc aacaagtacc atacttggtg
    28081 agcctaaatc gatgccgtag aaagatgatt cccggatacc ttctgtataa cgtgccgtaa
    28141 agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg attccgccgg
    28201 tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct gccatttgtt
    28261 cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt ggttttaacg
    28321 cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct agtggcaaga
    28381 tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac caattgaaat
    28441 tttcaactag atatgtaatc gcttggccca taggatggac gagtactcga taaacgattc
    28501 ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa aagagcagga
    28561 tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa tacttcctct
    28621 tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc aatcaacttc
    28681 atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttga aaaaaagaca
    28741 aaaaatcaag aaagaaatac taggaaaaag tagaatgatt ccaagtgttt ctttcttact
    28801 ttaataaact aaaaactttt tttctgcccg aaaaagcgga gtcaaatgaa ctcactgcaa
    28861 gtgatagatc agctttttca ctgctgtcct taatttttga ggcaaagcgc ctcaagaata
    28921 accttatcga cggtgttcaa aagcttgcat ctacggcatt aagtccagcc tgttgaaaaa
    28981 tatggagagc tatttttcac aacctgtcct taatgcttga tgcagaccag ctttttcaca
    29041 accttatccc accacatcaa atttctttcg ttcctcaatc atcgggtctt cttggacatc
    29101 cacatactgc actcgtccac ttgggctcgg agatttcttg acttcttcaa taaatttctg
    29161 gatgatgtcg tcattgccga ttgcttcgat cgtgactgag ccgtcatctt cgttttttac
    29221 cgttccagtc acgcctagtt gatcagcgac catcttggtc atgtagcgaa atcctacgcc
    29281 ttggacgcgt ccttggacgt tcattttaac tttgcgcatg ttcgtcacct ttctttccta
    29341 ttttataaat taatgatacc gattttttta ggatgaaaca agctcaagca cctattccat
    29401 gatataatca tggggaggtg aggaatgtga aagagatcca atcagcaaaa aatccaagaa
    29461 tcaaagaatt aaaaaaacta cataaaaaga aatatcggga agaaaaggaa gaatatcttt
    29521 tggaagggtt ccatctgatt gaagaagctg cgtcgtctga tgcagaaata aaagaacttt
    29581 ttgtgacaca gcgaggcatg aaagaatggg gagactggct ggccgcaaac aaagagattt
    29641 ctacttttct agtttcggat gaggtcatgg atgctgtatc ggatctacct acaccacaag
    29701 gaatcttagc agtgatggcg ttaacggctg aaggcacgcc agatttttca ggaggatggc
    29761 tgttgttaga taatgtacaa gatcctggaa atgtagggac gatgatccgt acggcagatg
    29821 catttgatat ggctggtgtt ttattaggca aaggaacggc agatatttat agtacaaagg
    29881 tgctgcgcag catgcaggga agcaattatc atttgcctgt gttgcaaaaa gatttgacag
    29941 aagtgatttc tttaatgaaa gacaaagggt ttacagtgta tggcacagaa ttaaatgaag
    30001 aagcacacgc attagacaaa gtagaaaaat cagataactt cgcaataatc atggggaacg
    30061 aaggtcaggg ggtaagtcag gaaatcttgt ctttgactga tcagaacgta tatatcccga
    30121 tgaaaggaaa tgcggaatcg ttgaatgttg gggttgcggc agggattgtt ctttatcatt
    30181 tttctttatg attaaggaac tattaagctt tggctatttt atccaaggcg actagaataa
    30241 tccgaacact ggtggtatac ttagatcaat ttcagaagaa aatgaaattc aaaaaggtgg
    30301 cagatcgata attatgacag aaaaatggcg tgaagacgaa gaatatatgt cttatgtaga
    30361 agatctttta gaaacagaag cagtgaaaaa attagcgaat tacacacagc atgttcattc
    30421 cactcgtttg gagcattcca tcagtgtttc ttactatagt tatctgctcg caaaaaaatg
    30481 gggtggcaat gctaaagcaa cagcgcgtgc tggacttttg catgatctgt tctactatga
    30541 ttggcgtacg acgaaatttg atgaaggcac acatgcgtat atccatccaa gaattgctgt
    30601 aaagaatgca gaaaagatca ccgaattgtc ggatcttgag cgagatatca ttttgaagca
    30661 catgtggggc gcaacgattg caccgcctaa gtacaaagaa ggatatatcg taacattcgt
    30721 agacaaatac tgcgcagtca aagaagccgc tcagcctatg tcagcaagta tgcgtaagag
    30781 atggcaacgt tactttggga aagaatcctc gatttaatag ataaaaccaa taatagacca
    30841 tattgaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg
    30901 cttctcaaat ttttgtgaag tttcggctta ttaccgcaga tgccagcttt tgaacaccat
    30961 tcattcgaaa caagggactt tgacacgact tttgtcacag tccttttttc tgtttgcagt
    31021 tgactatctg ttcaagatac ggtttacttt tatttatcga atgaatcatg tacatagaat
    31081 aataagcatt aaagggggtg atgataagat gcaaaacgta tcttatttaa aagagtccgg
    31141 gatcttcagg taacacatac tttgcagaag atcacttttt tctacaaagt atgcactttg
    31201 gttagaagaa aggactttaa aatcatggaa caaacaataa aaccatacca atattttttt
    31261 ataataagag aagtagaaca gttattaaat gcgtactcat ctgtcaatga tccaaaaacg
    31321 gtacaaacag ttcaggcact agcagctgaa aagattcgag acatattaga tcacgaactg
    31381 ccagaatcga tacattttta acgacagttt tagacgtaaa actgacaaaa gcgcaagcag
    31441 aaagagcatt ggaggagctg aagcaaatcg ttcagccgtt tatgcagcct agcaaaccac
    31501 aaatcgagaa attattccga aaagtgaaaa aactcaaaca accagcttgg aatgaaatcg
    31561 acttgcaaga acatacgtac atcggctgga atgatccagg tacgcagaaa aaattcatgg
    31621 tctattacga agatgaaaaa ttaaaaggga ttagcggtac tttatcacca acgataaata
    31681 agggaatctg tgcaatctgt cagaaaacat cgaatgtttc gctttttctt tctacaacga
    31741 aagcaggttc tgatggcacg tatacgaaaa aggggaatta tatttgtcat gatagtgacc
    31801 aatgtaatca acagttaacg aaattggctc ctttctatga atttgctgaa cgtgtaaaga
    31861 aagaacgata attttattgt tttttacaga aaaatatgct atcttttaat ggaagtgaat
    31921 aggtgatggt gttcgccttt aaatgctaac gaaagatagc taatgacgcc tactaaagtc
    31981 tggggacagc ccctttcttt agtaggcgtt tttttattga ggaggaattt tttatggaaa
    32041 aacaattacc gagtccagta cgcattttag tgtatatgct gaagtggctg gtgatcacag
    32101 cgattctcgg tatattcatg gggagcttgt ctgctttttt cttaaatagc ctgacagttg
    32161 tgacagatat ccgactatca catccttggc tcctttgtct actaccagtt agtggtgcgc
    32221 tttttgctta tctctatgcg tatcacggcg gactttctag tcgtggaaac aacttagtga
    32281 tcgatcaagg aaatggtgga gaagaaaaaa tcccgttgcg gcttattcca cttacactgt
    32341 ttggaaccat aactacccat ttatttgggg gatctgttgg ccgtgaagga acagcagtcc
    32401 aaatgggggg agcgttagca gataatacgg cacgtttgtt ccgattggat aaatcagaac
    32461 gggaaatctt gattatcagt ggaatcagtg cgggttttag ttctgtattt ggcacacctt
    32521 tagctggaac attatttgga ttagaagtgt tggcaatcgg gaaagtccga acagaagcga
    32581 tcttccctag cttctttgct gcactgtttg caaatttcat gacagaaagc tatggtgttt
    32641 ctcatctaca ttaccaaatg ggtgtgattc ctgaatggag tatgttgctg ttcttcaaag
    32701 tttttgttgc tgggattgct tttggtttga ttggttgggt attcagccgt tcgatcgtct
    32761 ttttaaaagc gcgatatgca caatggattc ctcatcctgt ttgtcgaaat acgataggtg
    32821 gagctgtagt tgtggcagca gcattgattt tgcaaacaca acgttatctc ggattgagtc
    32881 ttccgttact tcaagaatca tttaatggac aggcacatgt ctatgatttt attgggaagt
    32941 tattcttcac agttttgtct ttaggcgcgg gatatcaagg gggagaagtg acaccattgt
    33001 ttgaaattgg tgcaacgctt ggtgcagcac tagcgccttt gctccatgtt tctgttcctt
    33061 ttcttgcagg attgggcttt atcggagtat tttctggtgc gacaaataca ccgattgcct
    33121 gctttgtgat ggggatcgaa ttgttcggtt cacaagcggc agtatacttc tttatgatct
    33181 gcttgatcag ctttatgtgt tcaggcaaca gcggtattta ttctgctcag caagtagcgg
    33241 tcaaaaaagg gatactcttt ttgccaatag ttaagagaat gaaagaaaaa aacaaggtgt
    33301 gacatatgtt ttgtcacacc ttgttttttt agaaaagtaa agtaatcagc gataagaatg
    33361 cggagcctgc gaaaagcaat ccgcaaatga tcatatgtct ttttttgttc atcatcaaga
    33421 tggcgaggta ttctcttaaa aacgcattag gcaagtagta gaaagctgtt tttgcaccaa
    33481 taccatttgc tttcaatttg gcttgccgcg cataaagacc ggctctaaaa atgtggtaat
    33541 tgttggaact aaagatcgcg cggtatggcc ctttcatttg ctgatccatg atttcctttg
    33601 aatagagcat gttttccaaa gtggttgtcg aattggtttc gacaagaatg tctctttcag
    33661 ggattccttg ttccatggca tattgtttca tcgcaatggc ctctggtact ttttcgtctg
    33721 ctccttgccc gccagacatc aataagatgg gcggatttaa tgtagcacgg ctttgagccc
    33781 gataaaaagc aattgcctta ttgattcgct tggctagaag aggagaaact gtttctccat
    33841 tgatcaatcc agcgcctaac acaacgatga agtcttgatt gtatcgaggc cggttgaact
    33901 gatacaagaa tgagacagtc aaaaagttgt agaatacgat aaatagatag atcaagatca
    33961 ggggaacgca gaaaaacaaa gcatttatcc attgcggcaa atattttaac aagaaaaagt
    34021 caaaaacaag aaacagtgta agaaaaatgg ctagtatcaa cgttaaaaga ttagctaatg
    34081 aatgcgattc tctacgtaaa actgtgaccc cgttccaaaa aagaaaaaca atcaatccat
    34141 aaataccaaa taaaaggaca agtagcaatg gaatcgtgat caaggcgatc agtccgccaa
    34201 gaaaaatatt ttgcgtttca tacagcaaga caaacaagta gatgccgaaa ctgatgagta
    34261 aaacattgaa taaaagacca ttcagtagcc ttcgtttttc tgtgaaataa gaaaaagcaa
    34321 agatactaaa gaacaacaat ggaatcaaaa aataaaaggt cgaataaaaa atttccaaca
    34381 cgacaaaata agttaagatt accgtaaatg tccacgtact gccgtaaaag aacaaggaag
    34441 gatccttctt ctgttttcgc caataaacga tagagaatac cccaataaga tagagaacta
    34501 aaaagatcag atctctggtt agattcataa attcaactcc tttttcttat acagtataac
    34561 gaaaatagat gaaaaaagct gttatttaaa ctagaaaaag caaaaaaaca tctttccgat
    34621 ggaaagatgt ttttcttatt gttcaattaa aacaagttgc cgaaaaattc tgcgacgcgt
    34681 cgccagccaa tcacgaatat attcgctttt tccactgttt tatccgtaat gatctctgtt
    34741 gatggtgcat tgttgtcttc taaatagcct aatttatcgt ctgccaaggt gatcgtagcg
    34801 gtaccgattt ttgtaccttt atccataggt gcttggatct cattgtcagt gacttgcttc
    34861 ttgtcaagtg ttggcgtgat cgttaatttg ccagtatcca tatcgtttcg tacccatact
    34921 tttacagggc ttttaagcac aacaggaact gttgattctt ttccgtcttt tacatcaatg
    34981 gtttttagac taggaatact tgcgttagct tttccgagct ctttttctga ccaattgtca
    35041 tagcagtagt ccatcaaacg gtctgtttca ttgaagcggg cactcgggtt ttccgcatgg
    35101 ccgtccgcat tcaatacgac agtgatgatc cgttgatcgt ttttagtgat cgtaccaacg
    35161 aaacaagcac ctgcaagctc agtcgtacca gtttttaatc cgtccacgcc ttccttgtaa
    35221 ttaacgaatc ctgggagcat ccagttccag ttgaccattt ccactggtga ttgcgtattt
    35281 tcgccaaaca tttgggtcgt agtacttgat acatccagta tttctgggaa atccaagatc
    35341 aaatggcggg caacgatggc cacatcttga gcagacattt gattttcgtc gttttctcca
    35401 gtgccttctg gtcggttttc gccaagataa gagttgttca atcctgatgc attaacgatt
    35461 gttgcatctt tgattcccca atctttcaat tgttctttca ttcgttcagt gaatttagct
    35521 tcgctgccag agattttttc tgccaaggcc accattgaag cattggctga ctggatgatt
    35581 gcagagtcga ataattcttt gacagtgtag gtgttttctt tatgcaaagg aacattcgat
    35641 aaatctgggg tgatactcaa attttctgca taatctgaaa tcgaaacttt atcaccccat
    35701 gacagttttc cttcttttac ttgatcaagg acgatgtaaa gaccgatgat ttttgtgatt
    35761 gaagcgatcc ccatggtttt ttctccatct tgatcataaa gaatcttacc gctttgcgca
    35821 tctacagcaa aagctgcttt tgcatttact ttgaacgtat cttcagcttg cacagcaaca
    35881 gctggtagaa aagaagcaag gcaaagaatg agactagcca ataataggac tagacgtgtt
    35941 ttttttatgg acatatccga tctccttgta attaatttgt ttcgctacaa attaaaattt
    36001 tatcagagag gggagagagt ttcaatgttt ctgaaaaaac tttaggataa gaacgcgcgt
    36061 tatggaagtt tagcggtcgt ttttacgtac ttttacagga ataggctgtg tttccgcatc
    36121 taataagcgg gaagcgctga aagcaaataa actgataata cttaataaac tgaaaatcat
    36181 aatcaaatgc ataagatcca cctcttttat tctttttctt agtttatcag catttcatga
    36241 agaatagttg aagactgggg ctaaataatt gaggaaatta tgaagataac ttaaaaaaat
    36301 agctaaattt aaaaccgtag aaaccgtttt atttttttag atacgtttta ttgtatggaa
    36361 aaatagtgtt gtggtagact ttagatagaa acaaaggaat gattttttta ggaggggtac
    36421 gtttatggca aaaaaggata aagaattatt gacacaattg acttcagaag gcgctaaagg
    36481 accattcgtc acgatcatgc tgaatacgca tgtcgcccat caagatgtgg aaaaagatca
    36541 gatcaaattc aaaaattttg cgaaagaagc aaagaaacgt tttgagaaaa aatattccga
    36601 acatgactgg tccccgttcc aagaaaaaat tgaaaatttg ttaggagatc cttcgttttg
    36661 gcgtagtgca actacgagtg tagcggtcat tataactgct gaccagacct atatccatcg
    36721 tttgagtatc ccggtagacg atcaatacta tgtaagtgat cttccttatt tactggctat
    36781 tatcaaaaat gggcagttca actattctta ttatttattg ggattgaacc gcgactccat
    36841 gaaattgtat gaggtcagca acaaacaagt aaaagaagta tctctaccag aaggggcacc
    36901 ggttgatgtc gttactgcat tgggatctga actgacaggt ggcggtttga acttctcttc
    36961 taatggagga ggcggagccc gagatggagt aactttccat ggcgtaaatc caaaagatga
    37021 agaagtagag atcgattggg tcaactatta ccaagctgtt gatacgtact tgaaggattc
    37081 tttcgaaaat cctgaacaat ggccgattta tttatttgca ttaccagaaa atcagacgat
    37141 gttcaaaaaa attgctaaaa atccctatta ttgtgcagac gttgcgattt ctgcttctcc
    37201 tgcgcagcta tcctttagag atatcgaaga aggggcgaag aagctgtcaa gtgaactcgc
    37261 agctcaagaa tcaaaagaat acaataaact cttagataag aaattcatcg atcagttagt
    37321 tgatatcgtt ccagctgcga aagaaggaaa agtgtcacat ttatttatcg ctacgtcaaa
    37381 cttagctgat ggtttcggtg aagatcctga aacagaatat gatcggcgtc aagtgctgaa
    37441 tgcattagca gatgatgtga tcattaatgg cgggaaagtg tttattttgg atcaaaaaga
    37501 tgcgccagat gaaaagagtc tgacagcgat tcttcgttat taaaagaggt tgtgaaatca
    37561 gtgttttgac ctgagtatta gagaaacaaa cggaaaaata gtttcacata tttttccgtt
    37621 ttttgagcta ataccgaagg tcagctgatt aaacaccgtg tatgagaggt tgtgaaaaag
    37681 ctgttctgca tcaagtaata agaacagcaa gcaaaaatag ctcttcatat tttttgtttg
    37741 gtgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgacag
    37801 aagtggtcag cttcaagaaa taagaagaga ttgacaaaaa ttatttctca aatttttgtg
    37861 aaatttcggc ttatttccga agaagctact tctggaacac cgtttaaa
//
LOCUS       DAZKPO010000016        37736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000016 DAZKPO010000000
VERSION     DAZKPO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.016"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8NZ_000845"
     CDS             476..1339
                     /locus_tag="ACG8NZ_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0538540.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8NZ_000846"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8NZ_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0538541.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8NZ_000847"
     CDS             3339..3860
                     /locus_tag="ACG8NZ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0538542.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8NZ_000848"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8NZ_000848"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0538543.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8NZ_000849"
     CDS             5941..6759
                     /locus_tag="ACG8NZ_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0538544.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8NZ_000850"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8NZ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538545.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8NZ_000851"
     CDS             7228..7806
                     /locus_tag="ACG8NZ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0538546.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8NZ_000852"
     CDS             7901..8038
                     /locus_tag="ACG8NZ_000852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538547.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8NZ_000853"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8NZ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0538548.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8NZ_000854"
     CDS             9713..10738
                     /locus_tag="ACG8NZ_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0538549.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACG8NZ_000855"
     CDS             11061..11939
                     /locus_tag="ACG8NZ_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0538550.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="ACG8NZ_000856"
     CDS             12415..12531
                     /locus_tag="ACG8NZ_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0538551.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACG8NZ_000857"
     CDS             13459..13797
                     /locus_tag="ACG8NZ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538552.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACG8NZ_000858"
     CDS             13891..14121
                     /locus_tag="ACG8NZ_000858"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0538553.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACG8NZ_000859"
     CDS             14521..14892
                     /locus_tag="ACG8NZ_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0538554.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACG8NZ_000860"
     CDS             complement(15166..15858)
                     /locus_tag="ACG8NZ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0538555.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACG8NZ_000861"
     CDS             complement(15861..16646)
                     /locus_tag="ACG8NZ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0538556.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8NZ_000862"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8NZ_000862"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0538557.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACG8NZ_000863"
     CDS             18308..18625
                     /locus_tag="ACG8NZ_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0538558.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACG8NZ_000864"
     CDS             18638..20311
                     /locus_tag="ACG8NZ_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0538559.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACG8NZ_000865"
     CDS             20325..21956
                     /locus_tag="ACG8NZ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0538560.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8NZ_000866"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8NZ_000866"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0538561.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8NZ_000867"
     CDS             complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8NZ_000867"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0538562.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="ACG8NZ_000868"
     CDS             25190..25795
                     /locus_tag="ACG8NZ_000868"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0538563.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="ACG8NZ_000869"
     CDS             26103..27119
                     /locus_tag="ACG8NZ_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0538564.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="ACG8NZ_000870"
     CDS             27260..28561
                     /locus_tag="ACG8NZ_000870"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0538565.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8NZ_000871"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8NZ_000871"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0538566.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="ACG8NZ_000872"
     CDS             29290..29688
                     /locus_tag="ACG8NZ_000872"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0538567.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="ACG8NZ_000873"
     CDS             29754..30836
                     /locus_tag="ACG8NZ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0538568.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="ACG8NZ_000874"
     CDS             31048..32610
                     /locus_tag="ACG8NZ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538569.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="ACG8NZ_000875"
     CDS             32603..33736
                     /locus_tag="ACG8NZ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538570.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="ACG8NZ_000876"
     CDS             33736..34692
                     /locus_tag="ACG8NZ_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538571.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8NZ_000877"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8NZ_000877"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0538572.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="ACG8NZ_000878"
     CDS             35981..36799
                     /locus_tag="ACG8NZ_000878"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0538573.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8NZ_000879"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8NZ_000879"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0538574.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11761 a         6608 c         7967 g        11400 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtga
//
LOCUS       DAZKPO010000017        37546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000017 DAZKPO010000000
VERSION     DAZKPO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.017"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..2278
                     /gene="acm"
                     /locus_tag="ACG8NZ_000880"
     CDS             113..2278
                     /gene="acm"
                     /locus_tag="ACG8NZ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0538575.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2550..3035)
                     /locus_tag="ACG8NZ_000881"
     CDS             complement(2550..3035)
                     /locus_tag="ACG8NZ_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0538576.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3125..3470
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3515..4156
                     /locus_tag="ACG8NZ_000882"
     CDS             3515..4156
                     /locus_tag="ACG8NZ_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0538577.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4175..4810
                     /locus_tag="ACG8NZ_000883"
     CDS             4175..4810
                     /locus_tag="ACG8NZ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538578.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4828..5643
                     /locus_tag="ACG8NZ_000884"
     CDS             4828..5643
                     /locus_tag="ACG8NZ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0538579.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5962..7398
                     /locus_tag="ACG8NZ_000885"
     CDS             5962..7398
                     /locus_tag="ACG8NZ_000885"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0538580.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7587..10034
                     /locus_tag="ACG8NZ_000886"
     CDS             7587..10034
                     /locus_tag="ACG8NZ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0538581.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10264..11331
                     /gene="rlmN"
                     /locus_tag="ACG8NZ_000887"
     CDS             10264..11331
                     /gene="rlmN"
                     /locus_tag="ACG8NZ_000887"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0538582.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11321..11527
                     /locus_tag="ACG8NZ_000888"
     CDS             11321..11527
                     /locus_tag="ACG8NZ_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538583.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11524..12627)
                     /locus_tag="ACG8NZ_000889"
     CDS             complement(11524..12627)
                     /locus_tag="ACG8NZ_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0538584.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13180..13728
                     /gene="aac(6')"
                     /locus_tag="ACG8NZ_000890"
     CDS             13180..13728
                     /gene="aac(6')"
                     /locus_tag="ACG8NZ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0538585.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(13976..14095)
                     /locus_tag="ACG8NZ_000891"
     CDS             complement(13976..14095)
                     /locus_tag="ACG8NZ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0538586.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            14274..15935
                     /locus_tag="ACG8NZ_000892"
     CDS             14274..15935
                     /locus_tag="ACG8NZ_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0538587.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16005..16352)
                     /locus_tag="ACG8NZ_000893"
     CDS             complement(16005..16352)
                     /locus_tag="ACG8NZ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0538588.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16436..17377
                     /gene="opp3b"
                     /locus_tag="ACG8NZ_000894"
     CDS             16436..17377
                     /gene="opp3b"
                     /locus_tag="ACG8NZ_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0538589.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17378..18421
                     /locus_tag="ACG8NZ_000895"
     CDS             17378..18421
                     /locus_tag="ACG8NZ_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538590.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18427..19497
                     /locus_tag="ACG8NZ_000896"
     CDS             18427..19497
                     /locus_tag="ACG8NZ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538591.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19487..20440
                     /locus_tag="ACG8NZ_000897"
     CDS             19487..20440
                     /locus_tag="ACG8NZ_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538592.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20452..21462
                     /locus_tag="ACG8NZ_000898"
     CDS             20452..21462
                     /locus_tag="ACG8NZ_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0538593.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21747..22568)
                     /locus_tag="ACG8NZ_000899"
     CDS             complement(21747..22568)
                     /locus_tag="ACG8NZ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0538594.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22665..22943)
                     /locus_tag="ACG8NZ_000900"
     CDS             complement(22665..22943)
                     /locus_tag="ACG8NZ_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538595.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23211..25220
                     /gene="metG"
                     /locus_tag="ACG8NZ_000901"
     CDS             23211..25220
                     /gene="metG"
                     /locus_tag="ACG8NZ_000901"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0538596.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25699..26286)
                     /locus_tag="ACG8NZ_000902"
     CDS             complement(25699..26286)
                     /locus_tag="ACG8NZ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538597.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26461..27783
                     /locus_tag="ACG8NZ_000903"
     CDS             26461..27783
                     /locus_tag="ACG8NZ_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0538598.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27938..28714
                     /locus_tag="ACG8NZ_000904"
     CDS             27938..28714
                     /locus_tag="ACG8NZ_000904"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0538599.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28707..29276
                     /gene="rnmV"
                     /locus_tag="ACG8NZ_000905"
     CDS             28707..29276
                     /gene="rnmV"
                     /locus_tag="ACG8NZ_000905"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0538600.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29269..30153
                     /gene="rsmA"
                     /locus_tag="ACG8NZ_000906"
     CDS             29269..30153
                     /gene="rsmA"
                     /locus_tag="ACG8NZ_000906"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0538601.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30604..31233
                     /locus_tag="ACG8NZ_000907"
     CDS             30604..31233
                     /locus_tag="ACG8NZ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538602.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31357..32466)
                     /locus_tag="ACG8NZ_000908"
     CDS             complement(31357..32466)
                     /locus_tag="ACG8NZ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538603.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32675..33097
                     /gene="mgsA"
                     /locus_tag="ACG8NZ_000909"
     CDS             32675..33097
                     /gene="mgsA"
                     /locus_tag="ACG8NZ_000909"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0538604.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      33284..33382
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            33420..33956
                     /locus_tag="ACG8NZ_000910"
     CDS             33420..33956
                     /locus_tag="ACG8NZ_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0538605.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33996..35759)
                     /locus_tag="ACG8NZ_000911"
     CDS             complement(33996..35759)
                     /locus_tag="ACG8NZ_000911"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538606.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35762..37477)
                     /locus_tag="ACG8NZ_000912"
     CDS             complement(35762..37477)
                     /locus_tag="ACG8NZ_000912"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538607.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        12374 a         6630 c         7749 g        10793 t
ORIGIN      
        1 tcaatttttt agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg
       61 agagatgata tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa
      121 gtgtagaaat attattttat caatgttatt tattattact aatataacat cactgattcc
      181 tgttcatgtc tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt
      241 ggacccaacg aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa
      301 aaaacaaaat atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat
      361 tcgcggtgaa ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac
      421 tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga
      481 ctcagtagaa ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga
      541 agaaaatacg ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac
      601 aaagccagct tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc
      661 tgaagatact aagcacattc gatggttttt gaatattaac aataacggca cttatgttga
      721 acaaccagtt aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt
      781 tgaaattaat caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca
      841 acaattttta caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat
      901 tgaaataact ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac
      961 gatcatagaa aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga
     1021 atttaataaa cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc
     1081 ttctggtgga gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga
     1141 cacagagatt ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat
     1201 tcaaggacag tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg
     1261 cctgcaagta ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga
     1321 tccactaagt tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag
     1381 tttacctatt tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg
     1441 tggaaccact ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg
     1501 ggaaccagtt cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg
     1561 ggaagatatc caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt
     1621 agatcaaaac ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt
     1681 cgttacaaat gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa
     1741 tgagaaccaa gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca
     1801 accgatccaa cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct
     1861 gccaaaatac agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga
     1921 atacagtacg acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa
     1981 tatatctata ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag
     2041 agagaaaatt agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc
     2101 cagcgaacaa agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa
     2161 atcaaaaagc attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg
     2221 tttattgata gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt
     2281 agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata
     2341 atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat
     2401 ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt
     2461 gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg
     2521 aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct
     2581 aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata
     2641 tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt
     2701 tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg
     2761 aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct
     2821 agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct
     2881 tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt
     2941 ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt
     3001 agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt
     3061 agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt
     3121 aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg
     3181 gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat
     3241 agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc
     3301 tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat
     3361 cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc
     3421 tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca
     3481 agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc
     3541 gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc
     3601 attaccatta gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta
     3661 cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt
     3721 tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat
     3781 tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc
     3841 gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt
     3901 gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt
     3961 tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg
     4021 tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat
     4081 catctatctt acattgactg gtattctaac aatcataatg aaacaagttg aaggaaaatt
     4141 aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa
     4201 agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac
     4261 attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg
     4321 cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga
     4381 gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca
     4441 tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga
     4501 agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa
     4561 tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagctattg ccagagcatt
     4621 ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt
     4681 acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat
     4741 tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc
     4801 aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc
     4861 gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg
     4921 actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga
     4981 tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt
     5041 gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa
     5101 ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa
     5161 aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa
     5221 gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg
     5281 tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac
     5341 caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg
     5401 gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa
     5461 ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca
     5521 cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa
     5581 acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat
     5641 taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg
     5701 ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa
     5761 cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc
     5821 attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac
     5881 ataattttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg
     5941 ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt
     6001 ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa
     6061 ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac
     6121 ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa
     6181 caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga
     6241 ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa
     6301 aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat
     6361 gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca
     6421 ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc
     6481 ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga
     6541 atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac
     6601 gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt
     6661 gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat
     6721 tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa
     6781 aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca
     6841 gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga
     6901 gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt
     6961 cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag
     7021 attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg
     7081 gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc
     7141 catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt
     7201 tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg
     7261 ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa
     7321 ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa
     7381 aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt
     7441 gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg
     7501 aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg
     7561 aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga
     7621 aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg
     7681 ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa
     7741 tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt
     7801 tattagccgt attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa
     7861 accgacgctg gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc
     7921 atttatttgg caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt
     7981 tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag
     8041 caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag
     8101 tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa
     8161 atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc
     8221 aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag
     8281 caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa
     8341 aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag
     8401 agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga
     8461 atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag
     8521 atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa
     8581 atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa
     8641 gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt
     8701 tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag
     8761 tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc
     8821 ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg
     8881 gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac
     8941 aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg
     9001 tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg
     9061 ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg
     9121 aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac
     9181 ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag
     9241 tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt
     9301 ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct
     9361 taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg
     9421 aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga
     9481 tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac
     9541 cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta
     9601 atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata
     9661 aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat
     9721 ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg
     9781 aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg
     9841 tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca
     9901 accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag
     9961 gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg
    10021 ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac
    10081 aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga
    10141 tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt
    10201 gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat
    10261 actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata
    10321 gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt
    10381 gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag
    10441 cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta
    10501 aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa
    10561 tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt
    10621 gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc
    10681 atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt
    10741 gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc
    10801 atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga
    10861 ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc
    10921 ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca
    10981 attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg
    11041 tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca
    11101 gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc
    11161 agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta
    11221 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
    11281 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
    11341 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
    11401 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
    11461 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
    11521 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
    11581 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
    11641 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
    11701 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
    11761 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
    11821 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
    11881 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
    11941 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
    12001 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
    12061 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
    12121 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
    12181 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
    12241 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
    12301 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
    12361 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
    12421 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
    12481 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
    12541 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
    12601 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
    12661 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
    12721 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
    12781 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat
    12841 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
    12901 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
    12961 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
    13021 tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa
    13081 agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa
    13141 aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac
    13201 cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa
    13261 gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg
    13321 gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt
    13381 atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata
    13441 ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt
    13501 tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag
    13561 catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat
    13621 gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg
    13681 gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt
    13741 gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat
    13801 ttttgtgaaa ttccaactta tttccgaaga agctactttt ggaacaccgt gtaccagagg
    13861 ttgtgaaaaa gtcgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttctac
    13921 atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg tttattcaga
    13981 taagggactg tgacaagact tttgtcacag tttctttttg tttattcttt tttatctttg
    14041 tatggcaata atagacaata taacgaaaat tatttaatgg cttttggaag accatggaaa
    14101 accttgtaca aagctatttt ttctgaaaaa ttccttgaaa cctcacgaaa aaatcattat
    14161 tttatgaaaa agacaaaaaa aagtgttgat tttttaataa ttattcatta aactcttaaa
    14221 caacttcaga aaatattaat tattctgaaa attccaataa aggggtacgt aaaatgaaaa
    14281 agaaatttac atttggtttg gttgcggtat gcggccttgt acttgctgga tgttatggcg
    14341 gcagttcagc cacaagcagt agttcagcaa aaagcggttc agcagatgga ggaggcgtat
    14401 ttaacttagt cgtgcctcaa gaaatgccta cagctgactt atcagttgca acagatacaa
    14461 tcagttttac agcattgaat aacgtttatg aaggaattta ccgtcttgat gaagacagca
    14521 aaccacagcc ggcaggagct agcgagttgg cagaagtcag tgaagacgga ttaacttaca
    14581 aaatcaaatt acgagaagac gcaaaatggt caaatggtga tcctgttaca gcagcggatt
    14641 acgtttatgg ctggcaacga acagtagacc cagctacagc ttctgaatat gcttacctgt
    14701 atgcgccagt tgaaaatgca gaagacatta cagctggaaa gaaagataaa tcagaactag
    14761 ggatcaaagc agttggtgac tatgaattag aaatcaaatt gacgaaacaa acaccatatt
    14821 tccaatatct gcttgctttc ccatcatttt tcccacaaaa acaatccgta gtggaagaaa
    14881 atggggatgc ctatgcatct gcaagtgata aagctgttta taatggacca ttcacattag
    14941 aagattttga tggaccagga acagatacag aatggactta taagaaaaat gatcattact
    15001 gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta
    15061 ctgcattgaa cttattcaaa gatggccaag cagatgacgt gatcttgtct ggtgagttag
    15121 cacagcaaaa cgcaaacgat ccagcttata cttctgttaa agaagcgcgg acaagctata
    15181 tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa tgtgaaccta agaaaagcta
    15241 tttcttattc tatcgatcgt gaagctttag taaaacaagt actaggtgac ggatcagttg
    15301 tctctacagg attgatccca tcagatatgg taaaaagtcc aacaacgaac gaagactttg
    15361 caaaagaagc tggaaaatta gtttcttatg acaaagataa agcaaaagaa tattgggaaa
    15421 aagcgaaaaa agaattgggc gttgattctt tagaatttga tttgatggct tctgatgatg
    15481 attcatcgaa aaaagtaatc gaatatgttc aaaactcaat ccaagaaaac ttggatggcg
    15541 taacagtcaa accaacacct gttccattct ctgtacgtct tgaccgctca acttcgggtg
    15601 attttgacgc agtactaggc ggatgggctg ctgactatgc agacccaagc agctttactg
    15661 atttgtttgt cacaggtaat tcatacaacc gtggtcgctg gagcaatgca gaatatgacg
    15721 aagcagttaa agcagcttca tcaacagacg ctggcgatga acaagcccgt tgggatgatt
    15781 tccaaaaagc aatcaagatc atctctgatg atatgggtgt cgttccggtg taccaaaaag
    15841 cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt
    15901 catgggatta caaatggaca tacgttgaag aataatcgtt aataccgtga gcatgagaaa
    15961 aaacgaggct gcggcaaatg tcgcaacctc gttctcatat tcacttatcc taacaaagaa
    16021 aaaagcatga aaatagcaga aagagcaatt agtattccgg caatgatcag ccaaaagctg
    16081 tacataccgt agctggcaga cgagagcgag gaaaattctg gttttttctt tcggttcctt
    16141 gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat
    16201 aaaaaaccgc cgataatcag aaacggcaat gaccataaga atagatcatt agataattga
    16261 atgagtgtaa aggaatggtt gatgagattt tttattaaaa taaccagaat cagtagaaaa
    16321 gctactgcgt aggggataga tttttttttc atttgattgc tcctttattt ttggttcatt
    16381 atctaataat gtagcgaaac atctgtcaga agtcaaaagc tagggggaaa cattagtgaa
    16441 tagttatatc aaatatgtat taaaacgtgt ctttttcatg atcatcacgc tttggttaat
    16501 tgcaacaatc acctttttct tgatgcaatt gctgccgggc acgccatata cgaatcagga
    16561 acgtttgagt cctgaaacaa tcgagatgct aaataaacaa gtcggtttgg ataaaccagt
    16621 tatcgtacaa tatgggattt atctttctaa tttgcttcaa ggagattttg gaatttcctt
    16681 ccaatttaaa aaccagccag tcgcacattt gttagctgga agaatcggac catccttgca
    16741 gttagggtta caggcaatta tctttggaac agtcctaggg acgattttag gaacgatctc
    16801 tgccatgaag caaaatacat gggcagatac atcttctaca ctcgtagcga tattaggtcg
    16861 ttccattcca aactttgtat ttgctgtttt attacaatat atctttgcaa tcaaattaca
    16921 agttttgcca atcgcaaaat gggatggctt tgtgtacaca atcttaccaa caattgcgtt
    16981 ggccatgtcg ccgcttgctg actctgctcg gtttatccgt acggaaatgg tggaagtact
    17041 gcatagtgat tatgtggaat tagcaaaagc aaaaggattg agccgttggg aaatcgcctt
    17101 taaacatggt cttcgaaata gtttgattcc attgatgact ttattaggac cgttagctgt
    17161 agcattgatg accggatcac tggtggtaga aaatatcttt gctattcctg gtatcgggga
    17221 acaattcgtt aagtcaatca caacaaatga ttatcctacg atcatggctg taacgatcct
    17281 ttactcattt atgctgattt ttgttatttt gatcgtggat ctattgtacg gtctagttga
    17341 tccaagaatt cgtgtttcag aagggagccg aggataaatg gaaatgatac caaagcgtta
    17401 tgaaacagtt gcagatatcc ctgcggatga atttcttcca ttacaaaaaa ataccgaaga
    17461 agaacgtgaa cagattactg caccttcttt gaattttatc caagattcat ggcgccgttt
    17521 aaagaaaaac aaagctgccg ttatttcaat ggttttacta ttggtcatca ttttgatctc
    17581 tgtgatcaca atctttgttt ctcctcatga tccaactgcc caaaacgtgg cttatatcaa
    17641 tttgcctcct cgtattccgg gaatcaatat cgatggattg aatggaaaag caatggttgg
    17701 tggtgaactt gttgataaat atgctcaagc aaacgtccct tcaaacgtaa atttcttttt
    17761 aggaacagac ggtttgggac gtgatgtatt aagccgctta tttatgggaa tgagaatctc
    17821 gttgctgatc gcatttattg ctgcattact agatgtaacg attggtgtag cttacgggtt
    17881 ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg caacgttttt tggaaatctt
    17941 atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat
    18001 tttttcaatc gtggctgcta tggccatcac aaactggata ccaatggcac gtatcgttcg
    18061 tgcacaaaca atgaaattaa aagatcaaga atatgtttta gctggattga cattaggtga
    18121 atcaagaatg aaaattgcat tcaaacatat tttgccgaat atctctagtg tcattattat
    18181 tcaaatgatg tttagtattc catctgctat tttcttcgaa gcctttttaa gctttatcgg
    18241 tttaggtctt acgcctccgt cagcttcatt aggaacgatg ttgagcgaag ggtacaagac
    18301 attcctgtat ctgccttatc tattatggat tccggcagta actttatcgg ttatcatgat
    18361 tggctttaac ttacttgctg atggattgcg tgatgccttt gatcctaaaa tgaaagagtg
    18421 aagacgatga cgaaaatttt agaagtgaaa gacttagaaa tctcttttga tacgtatgct
    18481 ggaaaggtac gagcgattcg cggtgtcaat tttcatttga ataaaggaga aacattggcg
    18541 atcgttgggg aatctggaag tggaaaatca gtgacgacca gaagcatcat gcgcttgctg
    18601 tcaagcaacg ccaatataga tgctggtcaa atcctcttta aaggtaacga tatcgttgga
    18661 aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat
    18721 ccaatgacct ctttagatcc gacgatgccc attgggaagc aagtcgctga gtcccttaga
    18781 aagcataata aagtttcaaa aaaagaggga caaaaagctg cattagaatt gcttaaatta
    18841 gttggaatac ccgaagctga gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt
    18901 cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt atccggaaat actgattgct
    18961 gatgaaccta caacagcgct tgatgtgact attcaagcac agattttgga gctgctgaaa
    19021 gaaattcaga caaaaatcga tacatcaatt atctttatca ctcatgacct tggtgtagta
    19081 gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa gaatcgttga agttggaaca
    19141 tcggaagaaa ttttctacaa cccgcaacat ccctatactt ggggactgct tggttcaatg
    19201 ccgacattag atagtgcgaa cgatcgattg tatgctattc ctggttctcc accggattta
    19261 ttgaacccgc caaaaggaga cgcattctat ccaagaaatg aattcgcgat gaaaatcgat
    19321 gcagaacaag aaccaccatt ttttgaacta tcaaaaacac acaaggcagc aacttggcta
    19381 cttgctccgc aggcacccaa agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc
    19441 tttaaagaga aacaaaaaca tacttacggt caaggaggaa ttgctgatgc caaataaact
    19501 acttgaagtt aaaggattga aacagtattt caatgtagga aaaaagaatg aagtacatgc
    19561 tgtaaatgac atcagctttc acatttatga aggagaaaca ttcggtttag ttggagaatc
    19621 tggaagcgga aaatccacta ctggccgtac gatcattcgc ttgaatgaac ctactgatgg
    19681 tgagatatta ttcgacggtc aagatataac gaagatcaaa gataaaaaag gtttgacgaa
    19741 attccggcat gatgttcaga tgatctttca agatccatac gcttcgttga atcctcgtat
    19801 gaaagtcaga gatatcattg ccgaaggaat tgatgttaat ggactagcta aatcatctga
    19861 agagcgtgca aaaaaagtcg atgatctatt gagaactgtt ggattgaatc caagccacgg
    19921 tactcgttat cctcatgaat tttcaggtgg gcaacgccag cgtattggta ttgcacgagc
    19981 attggcagta aaaccgcgtt ttatcatttg tgatgaacca atttctgcac tagatgtgtc
    20041 gatccaagcc caagtggtca atttgttgca agatctccaa aaagaacatc aattgaccta
    20101 tctatttatt gcacatgatt tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat
    20161 gcataacggc ttgttattag agatgggaac aagtgatgaa atctataatc acggtgttca
    20221 tccttataca gaaagtttgt tatctgctat tcctctacca gatccagacc atgagagaaa
    20281 acgcagaaga atcaaatatc aacctgaacc agatgacgga caagtacgtc aacttcgtga
    20341 aatcgcacca gaacattttg tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa
    20401 actaaaacga caaaaggaat ctcttctggt tgctaactag gagggacata gatgctaaaa
    20461 gcttttaaat tccgtatgta tccgacagaa gaacaaaaac agcaattgat tcgtactttt
    20521 ggatgtgctc gatttaccta taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa
    20581 acaggagtag ccaatttttc gctcacaccc gctacattaa aaaaagaata cccattctta
    20641 aaagaagtag acagtcttgc tttagcaaat gcacagctta atttggatcg ggcttttcgc
    20701 aattatttca agggacgtgc cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa
    20761 tcttatacga ccaacaacca aacccgtacg atctatctgg aaaatggcta cttaaagtta
    20821 ccaaaacaaa aagaactaat caagataaat cagcatcggc cagttgaagg atcgattcgc
    20881 tccgctacga tttctgctcg atataatgaa gaattttatg tcgcactact ttgtgatgtt
    20941 tcgccagtaa aaaaagaatc tttagctaaa tggatcggga tcgcctatca tccgaagaca
    21001 ttgatccaaa catctcgacc tctagaagta actttaccaa aatttcacca gacagaagaa
    21061 aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc gctccgctca ccatagaaag
    21121 atcaggttag atcaggcaag caattatcag aaacaaaaga gaaaagtaat ggatctttat
    21181 ctgaaacaaa agaaccagcg agaagattat cttgaacaat tatctgggaa attggtcaaa
    21241 caatatgact acttatttgt agaatctttt ccaaaagaag aggcacatgc tgatttttct
    21301 attcacgact ggcataaatt aataacaaaa ttgcgttata agtcccaatg gtataataaa
    21361 aaatttcttt tgatcaatac cgacggagca gaagaaagca attccgtaag aaagagccaa
    21421 gtagtagaaa aaatgggcag acattcggtt atcaagggat aaaaaatgaa tgaaactgat
    21481 aggagcgtta gggttagcct tgttaataaa aagtcttaat gacttttgtt tctaggaatt
    21541 tttcttttga agaaaagctc actaattatg acaaaccaat aaaacaatag cagaaaaagt
    21601 tcgtacgagc tttttctgct attgttaatt ttttattcag aaagtttacg aatacatcaa
    21661 aattctgaaa caaaaaacgt aactcacaaa gagttgtttt tgcgtttttt cttgaatgat
    21721 taggtctgaa tcttttgctc acaaccttat tcatggtgcc ccggcttgag ctgatagatt
    21781 tttgaaggac ggccgattcc tacaggacgt tcaccgatct catcgaaata ttggagcata
    21841 gctttcttaa aattggaatg atcgatcgtt ttataatcga tccctaagaa tttagcaaat
    21901 acttttcgag cttccgtaat cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg
    21961 tgttccattt tattgactac acggttgaat gctttcaaga tgatttgact atggtcaaaa
    22021 gccaaagtat ctttgccaga agactctcca gtgactaaat caagcgagat agccacttct
    22081 ccgctagata aatggatctt gttactatga cgttctagtg tgaaccagcg tacttccttg
    22141 gcatcatctc cggcgattag cggttcttct ccaataaagg ctaagtagct gactgtgacg
    22201 acccagcctc gcggatctcg attaggcgta ctgaatgtat gcagttgctc aatattttct
    22261 tttgaaataa ccacaccagt ttcttccttt gtttctctta agacgctttc tccagttgac
    22321 tcatcctttt ggacaaagcc accaggcaat gcccatgaat ttcgataagg atgccctttt
    22381 ctttggatca acaaaacttt cagctgatct tcttctttgt tatagcacat cagtacgata
    22441 tcgacagtca aagagggctt gtcatattct ggacgttcct gtttttgata ccagttaaga
    22501 aattcttctt cacttgcttt atgttcataa tataattttt cttcttcttt agaagcaaaa
    22561 cggttcatct gaccacctcc attgacagta aaagtacttt ttaccttatt gtagcagact
    22621 ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta tcatttaaat atgaaaaata
    22681 aatggaagta tgagattgaa taaaaagaaa agcagataaa acggcaatac tttataccgc
    22741 tcttcacgaa tggcaaagaa gccacaaata acgatatata cgtcaatgat aaaacgcaaa
    22801 atccaagctg ctgtcataaa actgttcaaa tgcccatgga ggttaaaata accatacata
    22861 cgaatataaa aatagctgaa tattaaatgt gctaatgaaa gaaaagcaat actaagtgta
    22921 aaaaaaagat gtttcttctg catgattgtt ccagtccctt actaagatag atagtcttta
    22981 ttataccacg tgtgagggga gaaataaaaa gccgtttttt agtagatatg aaacaaaaca
    23041 aaaacccgca tttattcaac ctattcatcc ttttaaagga tagattgaac gtagatagaa
    23101 gtcgtttatc cagtcttttt cattttgaaa aaaagctaac agcatgcttt ttttatggta
    23161 tactagcaag gaaacgaatt cgcatcatgc gataaaggag gaagagcccc gtggcagaaa
    23221 aggaaacttt ttatatcact acgccaatct attatccaag cggcaaactt catatcggca
    23281 actcgtatac tacgattgca tgtgacgcaa ttgctcgtta taaacggttg atgggctttg
    23341 atgtgtttta tctcactgga gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag
    23401 aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc ggctgatgtc aagaaattat
    23461 ggaaaacgct tgatatcagt tatgataaat tcattcgcac gacagatgat taccataaag
    23521 cagctgttca aaaaatattt gatcgtttat tagaacaggg agatatttac ttaggtgaat
    23581 atgaaggctg gtactctgtt tctgacgaag aattctttac agaaacacaa cttgcagaag
    23641 tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag
    23701 aattggtaaa agaagaatca tacttcttcc gcatgagcaa atacgcagac cgtctattgg
    23761 aatactatga agaacatccc gaattcatcc agccagaatc tcgtaaaaac gaaatgatca
    23821 acaactttat caaaccagga ttagaggatc ttgcagtttc acgaacaact tttacttggg
    23881 gagtaccagt caagagtgat ccgaaacatg tggtgtatgt atggatcgat gcattatcca
    23941 attatattac tgcactagga tatggctccg aagatgaaag tctattcgaa aaatactggc
    24001 ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa
    24061 tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt atttgggcat ggctggctat
    24121 tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg
    24181 tggaacgtta tggtttagat gcactccgtt attatttgct tcgtgctgtt ccgtttgggt
    24241 cagatggtgt ctttacgcca gaagattttg tttcccgtgt caattatgat ctggcaaatg
    24301 atctagggaa cttattgaat cgaacgattg ctatgatcaa caaatattgt gatggactcg
    24361 ttcctgacta tgcctcactt gtcactccat ttgatagtga actgtctacg acggctgcta
    24421 acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga attcaataca gcattggctg
    24481 aaatctgggt gctgatttct cgtgcaaaca aatacattga tgaaacagaa ccatgggtat
    24541 tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt catgatccat ttagctgaat
    24601 ctttgcgtat cgcagcgata ttacttcagc caatcatgac agaaacgcca gagaagatct
    24661 tcaatcaact aggattggac tctgaaacaa tgaatcttga aggattacac tttggcgaat
    24721 ttccttcagg aacaaaagta gttgcaaaag gtacaccaat cttcccacgt ttagatatgg
    24781 aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg tactcaaaca aacgaagata
    24841 cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat
    24901 ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt catcaactgt caaaaagtag
    24961 aaggggcaga taagttgctg caattccgac tagatgctgg agacagccaa gaccgtcaaa
    25021 tcttgtcagg aatcgctgaa ttttatcctg atccaagcga attgatcggg aaaaaagtcg
    25081 tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc
    25141 tttctgcaga agcaccagat ggctctttac aagtaatcga agcaccaaaa tccatgccaa
    25201 atggctcaga aatcgcatag aaattcaaag ggggtatgac actagttgtg tcataccccc
    25261 tttttccgaa atttgagaag caattttcgt aaattccttc ttattacttg aagccaatca
    25321 tataatacag aaacagagaa ctatccaaaa taaatcctag aaaaagaagt acgagcttcg
    25381 ctgatttttg ttaatttaga ataatactaa taggaatcgt aaaaacagaa aaaaagcata
    25441 atgataaagg gtcgggcaga ttttaattga ttcatctggt actctttttt attgtcctta
    25501 cattcataaa gtttgaatgc aatgacttta ttacatctat atttatatct aacagtagcg
    25561 ttgatgtgtt aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt
    25621 gtgattgaaa atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt
    25681 tattttgtaa aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg
    25741 gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt
    25801 acgtccgata ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc
    25861 taaaagcaag gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc
    25921 catcactcca aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt
    25981 tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg
    26041 aaaaagccca accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat
    26101 caatgcaacg attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac
    26161 agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt
    26221 acgggaaaaa tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa
    26281 atccataaac tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag
    26341 aaatacatcc gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat
    26401 taaaatattc tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga
    26461 atgaaaggca aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga
    26521 accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct
    26581 acaagtttga cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg
    26641 acagtggctg aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa
    26701 gctgcgtatg gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt
    26761 cctgctaata ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa
    26821 ttatctaccg attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg
    26881 aatttgttag ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta
    26941 ataccaattg ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag
    27001 ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg
    27061 ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg
    27121 aaacggccgg aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc
    27181 gtttatctat tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt
    27241 aatttgaatg cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg
    27301 gtcactattg ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg
    27361 atccgcgtgc cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca
    27421 aagctttcaa agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct
    27481 tgtattatga tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg
    27541 tggctattca gtttactgat ttttatcggt gtctttattt taagaaaaaa agcgccggaa
    27601 ttgccacgtc catataaagt accactttat ccaatcgttc cgattattgc gattctcggt
    27661 gctattttca ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata
    27721 ggtgttacct tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag
    27781 taaaatattt caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt
    27841 tttctatttc aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc
    27901 ttatgtgcta aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca
    27961 tttaaatgct gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat
    28021 gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca
    28081 attaagccat gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg
    28141 cagttatacg aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt
    28201 agcattaggg gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga
    28261 taaagtattt cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca
    28321 tactagagaa gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat
    28381 cggtggaatc atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt
    28441 agggatgcat atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga
    28501 ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc
    28561 accagttcct tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa
    28621 gattgctgaa cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc
    28681 gcaccgatta tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg
    28741 ttgaaggaaa agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa
    28801 caatagggtc tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc
    28861 gtggagtgat cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta
    28921 tggaagctgt tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga
    28981 aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa
    29041 aggtcgtgac gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt
    29101 tagactatgg tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg
    29161 agttgcgcat cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta
    29221 ttacgccgaa agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat
    29281 cgtgaaattg cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt
    29341 aaaaaaagtt taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa
    29401 actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg
    29461 acggaacaat tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg
    29521 atccctgtat tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat
    29581 attttaaaga ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta
    29641 aaagtcgtag cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa
    29701 tcagatttgg ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc
    29761 tctgctgaac caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg
    29821 gaagccagtt tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat
    29881 tcagcgattt tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa
    29941 gcatttttcc gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac
    30001 ttgcagcatt cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa
    30061 acagctggaa tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca
    30121 ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga
    30181 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt
    30241 cgtttgtttg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt
    30301 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
    30361 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
    30421 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
    30481 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
    30541 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
    30601 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
    30661 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
    30721 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
    30781 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
    30841 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
    30901 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
    30961 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
    31021 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
    31081 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
    31141 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
    31201 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
    31261 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
    31321 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
    31381 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
    31441 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
    31501 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
    31561 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
    31621 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
    31681 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
    31741 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
    31801 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
    31861 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
    31921 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
    31981 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
    32041 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
    32101 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
    32161 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
    32221 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
    32281 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
    32341 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
    32401 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
    32461 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
    32521 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
    32581 gctagtttta agctttaata aatatccata agaaataaaa actgatacac tggagtaaat
    32641 aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga
    32701 ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca
    32761 tgaacttttt gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat
    32821 ccatcggttt aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga
    32881 ggatcagtta gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc
    32941 agatgtaaca gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat
    33001 cggaacagct gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat
    33061 cgtccatgaa atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa
    33121 cgtttaatgt tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata
    33181 atacgaacgt attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt
    33241 gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa
    33301 agcgttaagt gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg
    33361 ttttattttc gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa
    33421 tgaagaaaca ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga
    33481 tggtcacttt gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat
    33541 ttattccaca agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag
    33601 ttttagctga tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta
    33661 cattgaatgc atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca
    33721 cttggaaaaa tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga
    33781 caccattatg gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc
    33841 cccgcctgtt aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag
    33901 gacgtagtat tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa
    33961 caagcagtct tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga
    34021 tagagccgtc ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca
    34081 atagctccct gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg
    34141 gcaatcacaa aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc
    34201 tctgtcaatg tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg
    34261 atcatcgttc gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga
    34321 ccgccaatct tagtatctaa tttttccggc aaacgttcta caaattcata catataagag
    34381 gcttttaggg catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt
    34441 tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt
    34501 aatgtatcac gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt
    34561 gtgtcataaa aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct
    34621 actatagcaa tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc
    34681 ttggggtcat aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt
    34741 tccaaggcat aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat
    34801 gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata
    34861 aatggtttag aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt
    34921 gacgaaccac tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg
    34981 tgatctacaa aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct
    35041 ttttcatata gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga
    35101 aaagctttca cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca
    35161 ataatccttt gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca
    35221 aggaaaataa ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc
    35281 ataaaaataa acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca
    35341 gaaattgcaa tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg
    35401 gagttttgat cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt
    35461 aattttgcaa cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg
    35521 actacgttga ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag
    35581 cggacctgtc cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca
    35641 actgttgcaa tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag
    35701 gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata
    35761 actattcttc ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt
    35821 cttcatgggt accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt
    35881 cttgaataga gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta
    35941 gcgccttacg caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa
    36001 tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc
    36061 cagagaaatt tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga
    36121 caaattcttc tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt
    36181 tcttacccca ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa
    36241 caagagcaat ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca
    36301 ttataactgt accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt
    36361 tcccgcttcc ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa
    36421 tattttggag agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga
    36481 taattgcttc agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt
    36541 ctgtgtctag cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga
    36601 cgaggtttga aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa
    36661 gaacttgtcc ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac
    36721 agatgatccc gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg
    36781 aaacacgtaa ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt
    36841 tttttgtctg cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt
    36901 cattgagctg atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga
    36961 taaaaaataa aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc
    37021 agtcgatcac aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata
    37081 aacgaatgac cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga
    37141 tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga
    37201 ttagctgatt acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc
    37261 aaatgatagc acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa
    37321 tgatttttcc tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag
    37381 gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct
    37441 tacgatagtc ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt
    37501 gtccacaatt gaaaaaacaa gtactaccac attgtagctt aggaaa
//
LOCUS       DAZKPO010000018        36999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000018 DAZKPO010000000
VERSION     DAZKPO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.018"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8NZ_000913"
     CDS             <1..288
                     /locus_tag="ACG8NZ_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538608.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG8NZ_000914"
     CDS             347..787
                     /locus_tag="ACG8NZ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538609.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG8NZ_000915"
     CDS             799..1467
                     /locus_tag="ACG8NZ_000915"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0538610.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG8NZ_000916"
     CDS             1531..2154
                     /locus_tag="ACG8NZ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0538611.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG8NZ_000917"
     CDS             2210..2596
                     /locus_tag="ACG8NZ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0538612.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG8NZ_000918"
     CDS             2809..4200
                     /locus_tag="ACG8NZ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0538613.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACG8NZ_000919"
     CDS             4811..5179
                     /locus_tag="ACG8NZ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0538614.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACG8NZ_000920"
     CDS             5359..6300
                     /locus_tag="ACG8NZ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0538615.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACG8NZ_000921"
     CDS             6369..7100
                     /locus_tag="ACG8NZ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0538616.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACG8NZ_000922"
     CDS             7244..9424
                     /locus_tag="ACG8NZ_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0538617.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACG8NZ_000923"
     CDS             9444..9926
                     /locus_tag="ACG8NZ_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0538618.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACG8NZ_000924"
     CDS             9942..10703
                     /locus_tag="ACG8NZ_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0538619.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACG8NZ_000925"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACG8NZ_000925"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0538620.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACG8NZ_000926"
     CDS             12475..12960
                     /locus_tag="ACG8NZ_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0538621.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACG8NZ_000927"
     CDS             12953..13636
                     /locus_tag="ACG8NZ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0538622.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACG8NZ_000928"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACG8NZ_000928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0538623.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8NZ_000929"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8NZ_000929"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0538624.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8NZ_000930"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8NZ_000930"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0538625.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACG8NZ_000931"
     CDS             17933..18265
                     /locus_tag="ACG8NZ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0538626.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8NZ_000932"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8NZ_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0538627.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACG8NZ_000933"
     CDS             19091..20464
                     /locus_tag="ACG8NZ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0538628.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8NZ_000934"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8NZ_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0538629.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACG8NZ_000935"
     CDS             21519..21959
                     /locus_tag="ACG8NZ_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0538630.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACG8NZ_000936"
     CDS             22103..22804
                     /locus_tag="ACG8NZ_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0538631.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACG8NZ_000937"
     CDS             22801..23922
                     /locus_tag="ACG8NZ_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0538632.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACG8NZ_000938"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACG8NZ_000938"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0538633.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACG8NZ_000939"
     CDS             25301..26209
                     /locus_tag="ACG8NZ_000939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0538634.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACG8NZ_000940"
     CDS             26244..26516
                     /locus_tag="ACG8NZ_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0538635.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACG8NZ_000941"
     CDS             26527..27573
                     /locus_tag="ACG8NZ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0538636.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27824..30433
                     /gene="clpB"
                     /locus_tag="ACG8NZ_000942"
     CDS             27824..30433
                     /gene="clpB"
                     /locus_tag="ACG8NZ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0538637.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30584..31270)
                     /locus_tag="ACG8NZ_000943"
     CDS             complement(30584..31270)
                     /locus_tag="ACG8NZ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538638.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31248..31742)
                     /locus_tag="ACG8NZ_000944"
     CDS             complement(31248..31742)
                     /locus_tag="ACG8NZ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0538639.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31762..32982)
                     /locus_tag="ACG8NZ_000945"
     CDS             complement(31762..32982)
                     /locus_tag="ACG8NZ_000945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0538640.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33129..34964
                     /gene="lepA"
                     /locus_tag="ACG8NZ_000946"
     CDS             33129..34964
                     /gene="lepA"
                     /locus_tag="ACG8NZ_000946"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0538641.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35160..36071
                     /locus_tag="ACG8NZ_000947"
     CDS             35160..36071
                     /locus_tag="ACG8NZ_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538642.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        12482 a         6400 c         7919 g        10198 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    27781 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    27841 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    27901 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
    27961 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    28021 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    28081 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    28141 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    28201 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    28261 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    28321 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    28381 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    28441 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    28501 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    28561 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    28621 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    28681 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    28741 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    28801 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    28861 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    28921 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    28981 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    29041 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    29101 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    29161 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    29221 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    29281 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    29341 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    29401 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    29461 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    29521 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    29581 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    29641 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    29701 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    29761 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    29821 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    29881 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    29941 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    30001 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    30061 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    30121 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    30181 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    30241 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    30301 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    30361 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    30421 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    30481 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    30541 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    30601 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    30661 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    30721 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    30781 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    30841 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    30901 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    30961 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    31021 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    31081 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    31141 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    31201 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    31261 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    31321 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    31381 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    31441 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    31501 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    31561 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    31621 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    31681 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    31741 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    31801 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    31861 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    31921 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    31981 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    32041 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    32101 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    32161 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    32221 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    32281 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    32341 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    32401 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    32461 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    32521 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    32581 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    32641 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    32701 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    32761 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    32821 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    32881 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    32941 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    33001 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    33061 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    33121 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    33181 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    33241 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    33301 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    33361 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    33421 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    33481 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    33541 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    33601 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    33661 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    33721 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    33781 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    33841 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    33901 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    33961 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    34021 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    34081 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    34141 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    34201 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    34261 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    34321 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    34381 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    34441 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    34501 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    34561 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    34621 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    34681 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    34741 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    34801 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    34861 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    34921 tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa
    34981 aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga
    35041 ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt
    35101 cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca
    35161 tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc
    35221 aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag
    35281 ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa
    35341 ctctaataaa agatgaggaa gataagattg agattctcat cggtaaacta attgcataca
    35401 gagatatgaa taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat
    35461 tactcagtta tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg
    35521 atatttttct attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga
    35581 tatttagttt atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa
    35641 agaaccggga agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa
    35701 atgttataag tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata
    35761 ttgcctatct tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata
    35821 tacttccaaa agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat
    35881 taaggatgag gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt
    35941 tttctccttc aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca
    36001 atactaatcg aattatccaa tatgcttata taggaaatct ttctttagaa tttttgattt
    36061 acgggatata aaaatagttt acgtcgatac ttattaagtt atcgacgttt tttattatag
    36121 aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt ttgttgaaag
    36181 ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg caacacaagc
    36241 ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat gggctatgta
    36301 tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg ataagttata
    36361 gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca tgaaatatgt
    36421 tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat ctgtgaggga
    36481 atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata gtgggtagat
    36541 tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat ttatacagaa
    36601 ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt cttttttaaa
    36661 tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagcaatg aatcatagac
    36721 tgatatagaa taaaaaatga ttctatttat ctttttacat ctatcgtttt ttcaagtagc
    36781 cgcaaattat atcagcattc aacaattttt gtatacaagt tctagtaaaa ggatctgaag
    36841 aaaaagttgg ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt tttcttcaaa
    36901 atttgtgtta ttaggttagg acaaagataa gaagaagtat gaagatttaa aaaaggaatg
    36961 aggaaaagat agtaatttcc gattattgga gttttttta
//
LOCUS       DAZKPO010000019        36688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000019 DAZKPO010000000
VERSION     DAZKPO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.019"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..3345
                     /locus_tag="ACG8NZ_000948"
     CDS             67..3345
                     /locus_tag="ACG8NZ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0538643.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3886..5793)
                     /locus_tag="ACG8NZ_000949"
     CDS             complement(3886..5793)
                     /locus_tag="ACG8NZ_000949"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0538644.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5923..6798
                     /locus_tag="ACG8NZ_000950"
     CDS             5923..6798
                     /locus_tag="ACG8NZ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0538645.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6878..7852
                     /locus_tag="ACG8NZ_000951"
     CDS             6878..7852
                     /locus_tag="ACG8NZ_000951"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0538646.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8032..9177
                     /locus_tag="ACG8NZ_000952"
     CDS             8032..9177
                     /locus_tag="ACG8NZ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0538647.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9314..9658
                     /locus_tag="ACG8NZ_000953"
     CDS             9314..9658
                     /locus_tag="ACG8NZ_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0538648.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9929..11215
                     /gene="efmA"
                     /locus_tag="ACG8NZ_000954"
     CDS             9929..11215
                     /gene="efmA"
                     /locus_tag="ACG8NZ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0538649.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11353..11952)
                     /locus_tag="ACG8NZ_000955"
     CDS             complement(11353..11952)
                     /locus_tag="ACG8NZ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0538650.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12356..13477
                     /gene="mnmA"
                     /locus_tag="ACG8NZ_000956"
     CDS             12356..13477
                     /gene="mnmA"
                     /locus_tag="ACG8NZ_000956"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0538651.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13723..15144
                     /gene="arcD"
                     /locus_tag="ACG8NZ_000957"
     CDS             13723..15144
                     /gene="arcD"
                     /locus_tag="ACG8NZ_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0538652.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15158..16276
                     /locus_tag="ACG8NZ_000958"
     CDS             15158..16276
                     /locus_tag="ACG8NZ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0538653.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16336..17628)
                     /locus_tag="ACG8NZ_000959"
     CDS             complement(16336..17628)
                     /locus_tag="ACG8NZ_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0538654.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17778..18338
                     /locus_tag="ACG8NZ_000960"
     CDS             17778..18338
                     /locus_tag="ACG8NZ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0538655.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18467..18844
                     /locus_tag="ACG8NZ_000961"
     CDS             18467..18844
                     /locus_tag="ACG8NZ_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0538656.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18777..20849
                     /locus_tag="ACG8NZ_000962"
     CDS             18777..20849
                     /locus_tag="ACG8NZ_000962"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0538657.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21060..22007)
                     /locus_tag="ACG8NZ_000963"
     CDS             complement(21060..22007)
                     /locus_tag="ACG8NZ_000963"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0538658.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22253..22717
                     /locus_tag="ACG8NZ_000964"
     CDS             22253..22717
                     /locus_tag="ACG8NZ_000964"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0538659.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22779..23822)
                     /locus_tag="ACG8NZ_000965"
     CDS             complement(22779..23822)
                     /locus_tag="ACG8NZ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0538660.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24199..25164)
                     /locus_tag="ACG8NZ_000966"
     CDS             complement(24199..25164)
                     /locus_tag="ACG8NZ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0538661.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25398..26243
                     /locus_tag="ACG8NZ_000967"
     CDS             25398..26243
                     /locus_tag="ACG8NZ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0538662.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26434..27621
                     /locus_tag="ACG8NZ_000968"
     CDS             26434..27621
                     /locus_tag="ACG8NZ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0538663.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27627..27983
                     /locus_tag="ACG8NZ_000969"
     CDS             27627..27983
                     /locus_tag="ACG8NZ_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0538664.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27985..28323
                     /locus_tag="ACG8NZ_000970"
     CDS             27985..28323
                     /locus_tag="ACG8NZ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0538665.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28399..28655
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28718..29752
                     /locus_tag="ACG8NZ_000971"
     CDS             28718..29752
                     /locus_tag="ACG8NZ_000971"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538666.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29745..30431
                     /locus_tag="ACG8NZ_000972"
     CDS             29745..30431
                     /locus_tag="ACG8NZ_000972"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0538667.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30455..31303
                     /locus_tag="ACG8NZ_000973"
     CDS             30455..31303
                     /locus_tag="ACG8NZ_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0538668.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31500..32273
                     /gene="sufC"
                     /locus_tag="ACG8NZ_000974"
     CDS             31500..32273
                     /gene="sufC"
                     /locus_tag="ACG8NZ_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0538669.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32286..33572
                     /gene="sufD"
                     /locus_tag="ACG8NZ_000975"
     CDS             32286..33572
                     /gene="sufD"
                     /locus_tag="ACG8NZ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0538670.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33569..34804
                     /locus_tag="ACG8NZ_000976"
     CDS             33569..34804
                     /locus_tag="ACG8NZ_000976"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0538671.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34791..35261
                     /gene="sufU"
                     /locus_tag="ACG8NZ_000977"
     CDS             34791..35261
                     /gene="sufU"
                     /locus_tag="ACG8NZ_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0538672.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35266..36657
                     /gene="sufB"
                     /locus_tag="ACG8NZ_000978"
     CDS             35266..36657
                     /gene="sufB"
                     /locus_tag="ACG8NZ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0538673.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        11994 a         6781 c         7866 g        10047 t
ORIGIN      
        1 acagtaaaaa taaattaggt aatactttaa gtaaacgctt tctaatttgt tacgggagga
       61 atacacttgt taaaaaaacg tataaaatac agtgcgctaa tgatgaatct ccttatgctc
      121 agtaccccgg ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac
      181 gaagaaaatg cacacgtgaa cattgaaggg atgtcaacaa tggaggaaat accaagaact
      241 agtgaagata tggatacgga agaaggaaat gatcctatga tgaaagaatc agaaaatgaa
      301 aagacaaaaa agcaatctga agaagcaaat gaagagtgga aaggggttgt tttcggagaa
      361 agcacaagtg ctagtaggta tgccattgaa ccattagaag acggcgtgcg gctctcttcc
      421 acgaataacg gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta ttattatcaa
      481 gcaatcccca aagacatcaa ctttaggatg cgggcgacga tcgaaataga atcttggacc
      541 tatacgaatg cccaagaagg atttgcttta atggtaagag acgcagtacc aaaaaatcat
      601 ttgtttgggc aggcatttta ttcaaattca tttgccatac taggcagtcg gatcgaatat
      661 gtctgggact cggatagaca agaagtggtc aacacgagcg gcagcaaata tacgatgcga
      721 cttggattgg ggacacgggc aatcacagga atatcatctg aagatccgca agctgcgcca
      781 gctcctggga gtgtctcagt ggaaacaaca cctttagaga cttcagcagg gttcttggaa
      841 aaagaaacag gttcttataa tatattcgga aacggtcatg ggaatctgtt tccggccacg
      901 aactcgatta ctaaactgga cgtagagatg aaaaaaacga atactggttt agaggcatat
      961 tattacgatc ctgaaacagg agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa
     1021 ttatacgctt cagatgaatc ggttatttat gcaggatttg ctgcagcaag gaatatgacg
     1081 atcaaggtag aaggaatcga agttgcttac tcagatcctg cgacagatcc tcctgggcaa
     1141 gaaagaccta ctcgcttgat tgatccgatt tacactgtga cctctagtaa tcattcagga
     1201 aaccaagatc atactatgtc ctttagagca aatgctgacg gattattgac aattaaaaat
     1261 aaagcaacag gagaggtgct taagaacgca aaaaacttgg ctatcactgc gaatcgggaa
     1321 tttgattatc agacaaaact gatggaagga acgaacgaat ttactttcag ttttacgcca
     1381 gataaaaact atccgccaga agaatacgta gagatgacta gttatgaaac aaaagagatt
     1441 cttcatacag tggactaccg taaacctaaa tattcgacag tttatgtaac accagaaggg
     1501 tcagatgcag gaaacggaag cagacaaaat ccattatctc tgacacaagc attgagatac
     1561 gcatatgctg ggcagacaat cgtcatggcg cctggaactt attccttcga aagaggattg
     1621 acgattccaa aaggcgttaa cgggacaaga gaaaacccga ttcagttgat tgcagagaaa
     1681 aatccagaaa acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg gatgacactg
     1741 ttcagtcact actggggttt acgagggttt gacattacaa atagtgcagc tatgcaaaaa
     1801 ggattacaga tttctggaaa tcataatttg atcgaagaga ttcatgctta tcggaatgga
     1861 aatacaggaa tccaaatcag cggtagctca aatgatcctt tcgaagcatg gcccgcgcat
     1921 aacttagtca agaactgtac atcttttgaa aatgccgatc caggatttga agatgccgat
     1981 ggatttgcgg ccaaactgac aataggagaa ggaaatgtat ttgacggttg tatcgcgtat
     2041 cataatgcag atgacggttg ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa
     2101 gtaatcatca aaaactcagt tgcttatcga aacggctggg taccaggaat tgagggagaa
     2161 ggtaacggta atggattcaa aatgggcggt tcttctttaa cgggccctca tgaattgatc
     2221 aatagtttgt ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt
     2281 atcgtcaata gcagtacaag ttttaataac ggttcatata atgtggctct ttatacctct
     2341 tcagcaggaa acacagacta ccatgcttct ggtatcttat ctttccgaac agagaatctt
     2401 gagatgagag agcagatccg cccattgaat caaaacgaag atcaaatcta ccactcgttg
     2461 aactactatt ggaatgaacg agagaaacaa tcagaaaata ctcttggaca taccatatca
     2521 aaagattgga tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc
     2581 tatgaaaatg gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa
     2641 acagaaaagg aaagagtgga tggtcttcca ttatctgtag gtgctatttt tgacggagaa
     2701 acctcaccgt cttcggaata tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa
     2761 ccaaatgaac aaaataatcc gattattata caagaagcaa atggtgtggc ggagaatgca
     2821 gaaggaaata tcattgaact gaagcctttt acagtttttg tgtcaacttt cacaccaaat
     2881 caaggaacgc ctgtctttga agatatgcgt tatttcccag aaaagatcca aatcgttgaa
     2941 acacagtcaa gtgctcaatt ttctaatcat accgctattt tttcagcacc ttcaaaagga
     3001 gaatatacgc tagaagtcac ttatcgttcg gaaatatttg atggggaaaa atggtctgct
     3061 catacagaat ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc
     3121 ccgggaaatg acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca
     3181 aatggaaaac cttctcatcc ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa
     3241 ggactcccaa aaacgggaga atttaataac ccgttgctag cattagctgg aggaattttg
     3301 ttaatcggtg tagttgttta tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt
     3361 ttatgatgta aaacaattga ctgaggaaag taaaaataga aggaaatgag accaatagat
     3421 gtctcacttc cttctatctt tttatagatg aggttctcct tgctgctttt gattggctgt
     3481 tcttattact tgatgcagga cagctttttc acaatctctc cactaaacgg tgttcaatca
     3541 gctgaccctc ggtattagct caacaaacgg caaaatatga ggaactattt ttccgttttt
     3601 ttctctaata ctcaggtcag gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa
     3661 gccagtttct cggtagtaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
     3721 tttttcttac tacttgaaac taagcagctt tttcacaacc tctaatacat ggtattccag
     3781 aagtaacttc ttcgaaaaat aagccgaaat gtcacaaaaa tttgagaagc gattttccca
     3841 aattccttct tatttcttga agctgaccac ttctggcaca acctcttacc agattcgcac
     3901 tcttttttct ggcgctagat acatagcgtc ttgttccgtg ataccaaatg tttcatagaa
     3961 ttcgtcaagg ttttttggtt gcacgtttgc tcggagttta gcaggggcat ggacatcgat
     4021 ttgcagtaaa agttgttggt attctttttt tgcttttgta cgccaaatcg ttgcccaatt
     4081 gacaaagaat tcctcgatag aaagatctga ttccgttttt gctgcttcca atgcacagct
     4141 tagaccgcct gcatcagcaa tattttccga aacggttaat tttccgttta cttttccgtc
     4201 agcaaaaggc agaccatcga attcttcgat cattgcttgt gctttgtttt gaaattgtgt
     4261 aagatcttct tctgtccacc agttgttcaa gttgccatat tcgtcaaata aagcgccgtt
     4321 attatcaaat gcatgggaaa tctcatgagc gataacagct ccgattccac cataattagc
     4381 agagcttgat tgttctagac tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat
     4441 cacattccga aatggatgat aataagcatt gaccgtgtca gcactcattt cccattctga
     4501 acgatccact ggtttgttcc atttgctgaa acgatctttt aatgtgagtt tgctgaaaac
     4561 aagagtattt gaaagcaaag taccaccctg atctgcaggt actgttttga actggtcgaa
     4621 taatgctggg atcttatcgg ggtagcctac ttggatacct aagttattta attttatgac
     4681 ggctttttct tttgttgcat ccccaagcca gtcattttct tctagacgtt ttttataaac
     4741 ggcgatcatt tttttcacca tttgttctac gtctttttta gcagcttcgc caaaatattt
     4801 ttttccgtag taatcgccaa ctacttgatc gaattgtcca gaggcaaggt aatacgctgc
     4861 ttttttctga ggcattgctt cgtctgttcc agacaatacg cgagaataga ttccgctgac
     4921 ttggcggaat tcttctgata gataaccact aagagaacgt actgttttaa tgatcatcca
     4981 gcttttgaat aacggaaaat gagcttctgt caggatgtca ttcaaatgat cgaagtacac
     5041 tggatcagtt acgatcactt tatctggagt tgtcccaatc aaacccgtga caagtttttt
     5101 caagtccagt ttatctgtgt atttaataaa tgtatcaaaa gattgcggat tatacatttt
     5161 gctgtaatct gcgttttctt ctgcactttt tacatgagga gcaatcaatt tatcaaattg
     5221 cactgcttgt tccgtgattg cttctgcctg acttttttct ttgccggcaa gctctaataa
     5281 ctgaacagcc atatcaaaga aaacgcctat tagttgtgca ccgttgggat ggtctgatgc
     5341 ataatacgtc ttatctggaa ggaataaaga aggcggataa gcaaagaagg cgttcgtttg
     5401 cgcattcttc atatctgcat ctacatccag attaaatggc aatggtaatg tatccaacag
     5461 ccattctgga aattgcttat tcaattcgtc gaaagactgc aatgattcaa ctttttcgat
     5521 tataggttga agcggttgag catgcaactc atttcttcgg tcataatcgt ttgccagttg
     5581 ataatatgca aggaaatgtt tcattttttc tgaagacact ttttctggat gggcaagcag
     5641 atcgtctgtt tctcccatca aaagttggtc gatcccgtca actaaatctt ggaaaccgcc
     5701 tgtagctggt ttatctgcag ggatttttgc tgttttttgc cattcttcat tgactgcttc
     5761 atagaaatct tgtttcaatc gttctttgtt cattcatcga tcgctccttt tttgtttcgt
     5821 gtgcccatta tcttacaaaa tgaaggaatt aacaaataga acataaaaaa ttgaaaattc
     5881 gaacaatcaa actgatttgt ttttttcaat ccttcactta taatgaaaaa aaggaggcga
     5941 tcccaactga atcaagtagt ggataaacct ttatatttca aagtagataa aaaaattaaa
     6001 aaattagcca gcactcagca gctgcagtca agaaaaagta aattgctttt tttagctctg
     6061 gtttttgaag atcaaagtta tgtgattatc gatcaatcag gacatccggt agaatattct
     6121 ccagcaaaat atacttatca agagggtctt tcttgtaaaa aatggaagct gatcaatgaa
     6181 gagccaatcg agttatctag atggatcaac agaaaagaag atattcccgt tttgatcgaa
     6241 gaaaaaaggt caggaaagga actcgcaaat tgctgggtgg gattgccaga agaacgtttt
     6301 ttgaggtata agaaatgggc taccccctca ggctatcttt gtggtactta cgcggcagca
     6361 gttctccttg cctattatca agactatcgt aaggggtgga tgcttccaaa tgagattcgc
     6421 aagaaaaata cggctgattc gttggttttg acgaaggcat tgcgtagcca gatccaacca
     6481 ctaggacttc ctacgattcc ttttcaggtt agtacaggga tttctagttt tttaaagaag
     6541 aatggcaatc atgagcgtgc acgagcaact cttctaggtt catggcagcg agctacaaaa
     6601 cgtatacgtg aaggcaaacc tgtgatgatt ggtattttga aagtacttgg cagtacatat
     6661 ggcaatcatt gggtgacagc gtacgcttat tttgaaactg aaacaggaga acgttattac
     6721 aaagtacacg acaactgggg cgattatcat aaagtcattc ctgccagttg gagtaacgga
     6781 acagtttcac ttccttaaga atttgctgaa attaacagaa aatcttggaa aaatatgtta
     6841 aactatcgaa gaatagagaa gttttgggga ggactacatg agtaataagt atcaggatga
     6901 cacgttgaag atttttagtt tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt
     6961 gttcggaaca gaattaggta aaagcgtcgt gaagcaattt agtgatggcg agatttccat
     7021 caatatcgaa gaaagtattc gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc
     7081 tgtcaatgat tactatatgg aactactgat catgatcgat gcaatgaagc gagcaagtgc
     7141 gaaaacgatc aatgtcgtat tgccttacta tgggtatgct aggcaagacc gcacggcgaa
     7201 acctcatgaa ccgatcactg cgaaattgat cgctaacttg atcgaagaag caggggcaac
     7261 tcgagtactg actttagatc tgcacacggt tcaagtacaa ggattctttg atattcctgt
     7321 agataattta tttacgatgc cgttgtttgc tcattattac cgtcaattag gtctgacggg
     7381 agatgacatc gtagttgttt cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc
     7441 agaatatttg aacagcacat tagccatcgt agatcatgca gatgatgctt caactgaggg
     7501 tggttatgtg atcggtgatg ttcaaggaaa gacatgtatc atggtagatg atatcttgaa
     7561 cacaggagca acgcttgcac gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt
     7621 atatgcctgt gcgtcacatg gattattgtc cgatcatgcg aaaagtattt tagacaatgc
     7681 accaatcaaa gatatttgta tcactgattc tgttttcaca gaagaaaatc gtcatccaga
     7741 aaacttaaca atcgttacat gttcagaatt aatgggtgaa gcggtcaaac gcatccatga
     7801 aaatacaccg atgagtccgt tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa
     7861 aagagggaaa gaagttgtga cagaagtgag cagcttcaag gagctgcgac aagacttttg
     7921 tcacagctcc ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt
     7981 ggtttgttcg ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa
     8041 gtgaaaacta tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa
     8101 gcgatgacgg caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt
     8161 cgccacgcgc atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca
     8221 aaacctcacg agatcatctt taacagtggg ggaacagaag gggataatac agctatctta
     8281 gaaacagcgt tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat
     8341 cctgctgttc ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg
     8401 cctgtagatg aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat
     8461 acgatcttgg tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa
     8521 gaaatcggtg aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg
     8581 tacggaaacc aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct
     8641 cataaaatca atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc
     8701 cctgcattgc ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta
     8761 gcagggatct ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca
     8821 cgtaacaaca aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa
     8881 atcccttatc aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc
     8941 ttaattggcg aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt
     9001 tctactggtt cagcttgtac agcaggaaat atcgagcctt cacacgtatt agaagcaatg
     9061 tatggagaag atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat
     9121 accgaagacg acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta
     9181 gtgaagtcat tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt
     9241 aactgaaaaa cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat
     9301 cgaggtgaaa atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta
     9361 tcgtttgcat cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa
     9421 cggaggcaat tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt
     9481 caaattaaaa atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc
     9541 aaatggattg aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa
     9601 attttatttt ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc
     9661 tgtggatata aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta
     9721 aggacgacgc aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta
     9781 atattttgct gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta
     9841 gggaaaaagg tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata
     9901 caaaatgaaa aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa
     9961 ttggaagaga aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat
    10021 ggtcgtccaa tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt
    10081 gagttttgct actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc
    10141 gctagtggat cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat
    10201 tttcgcgctt attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt
    10261 ttttgtgtct ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc
    10321 gattatgccg acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat
    10381 ggtccaatca gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt
    10441 tcctgtcaat taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt
    10501 gatttttgtg aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt
    10561 taaagatgca aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac
    10621 gatcaatggt gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat
    10681 gacgctagat tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc
    10741 agtcggaatg ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat
    10801 gaaacctatt attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt
    10861 tccaaacgat agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc
    10921 aacgccatat ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt
    10981 tttaggtcgt gtcttaggga acttcaactc tttgatgaac ctggcaggtc caattggatt
    11041 attatttgca ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg
    11101 aatcgggatt ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga
    11161 taaagaatta cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg
    11221 aatggagttg tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc
    11281 aataagccta ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat
    11341 agggcagttt tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg
    11401 ttgctcagat aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc
    11461 gtctacgcct acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg
    11521 aagattgcgg atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg
    11581 ttgatggtcg aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc
    11641 agtaagccta ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc
    11701 tttgatcaag tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc
    11761 tttttcatat tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt
    11821 tattactgca ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag
    11881 ccgcggagca aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat
    11941 aaagtaattc atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct
    12001 gagcaactca taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat
    12061 aagaagcaat tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa
    12121 ggctggccct ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat
    12181 tttttggttg actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga
    12241 cttgataatt aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag
    12301 ttgcgacctt caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac
    12361 agacaacagc aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac
    12421 agcacttttg ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga
    12481 tgacacggac gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt
    12541 agctgctcaa atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg
    12601 tgtatttgaa tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat
    12661 gtgcaacaaa gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga
    12721 ttatgtagct actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat
    12781 gcttcgtgga gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga
    12841 acagctttca aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat
    12901 tgctgaacgc gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat
    12961 tggtgaaaag aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat
    13021 ggtcactctt gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg
    13081 tcaacgtcaa ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt
    13141 aggaaaagat ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt
    13201 gtatgccaca agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa
    13261 agaattcaag tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg
    13321 cttattggat gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc
    13381 tggacaagca gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca
    13441 cgcatatcaa gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca
    13501 gctatggcta acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata
    13561 aaattaattt ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa
    13621 taatatgaat aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt
    13681 ttgcaagtga tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg
    13741 ggtattagca aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg
    13801 tttgggatca caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg
    13861 ataatcgttg ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag
    13921 cgtccagatc tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt
    13981 ggcttcatca gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca
    14041 acgatgctga tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt
    14101 ccttctattt tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga
    14161 gttgaaggag cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc
    14221 ctatttttaa tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg
    14281 ggtaacgtat ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag
    14341 attaaaggtt gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt
    14401 ttagccaacc gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt
    14461 tgtttattgt ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa
    14521 gagttagcaa ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat
    14581 tggggagcag cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct
    14641 tggacgatgc tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa
    14701 ggttggggaa aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc
    14761 ttgactaatc tattcttatt gactttctta gttactgatt atgcctatca atttgcctat
    14821 tctttatgta cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag
    14881 ttttctttaa aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt
    14941 gaattgatgg gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat
    15001 cttcctggtt tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga
    15061 aaagaaaaag taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt
    15121 gctgtaggct ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt
    15181 agaagaatgg ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat
    15241 cgaagcattg acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt
    15301 cgaaaaacgc aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa
    15361 acaagaagtc gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg
    15421 agcgactggt gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt
    15481 ttcgatgctt cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt
    15541 tgactttgtt catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc
    15601 cttagtcaaa cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac
    15661 attgctagat cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata
    15721 tgtattgata gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt
    15781 agatgtctat gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg
    15841 tattcgtatt ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg
    15901 agactacacg atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa
    15961 gaataaagaa aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt
    16021 aaaacaatgg gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac
    16081 ttctttcatt aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga
    16141 agaaacagga gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact
    16201 aggttactgt tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt
    16261 gaaaaaacag gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt
    16321 gtttttcatt tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc
    16381 tgaagctttt tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag
    16441 caagaagttg accaaaacaa gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc
    16501 actggaaaca ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg
    16561 gttataactg aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg
    16621 gacagcaggg agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg
    16681 ccaaacattc gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat
    16741 cgcaagtatt ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat
    16801 caaagtggca atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg
    16861 tccatgcagc aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc
    16921 ttggatgatc atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt
    16981 gaaaatctgt ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac
    17041 gaaaaacaga ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg
    17101 gcgatagcca aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt
    17161 aacagaacca gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt
    17221 tgcgacaagg gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa
    17281 tgcgactaat tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc
    17341 taatgaagca cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca
    17401 aggagagata agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt
    17461 caattgtgac gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac
    17521 taaactaaat ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc
    17581 ccaagtttct cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc
    17641 tttctgaaaa ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg
    17701 aaagtctctt ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta
    17761 tgagaggaaa catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct
    17821 ttcgagaact agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac
    17881 gcttggatat caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa
    17941 cagcgctaac agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg
    18001 gaaaaagtgg tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt
    18061 caaatcaagc agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat
    18121 tagatggaat ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa
    18181 cgaaagaaga aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt
    18241 ccttacgttt tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt
    18301 tttggatgat caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca
    18361 attttaagat gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa
    18421 aagtgactaa gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat
    18481 taccatgttc ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga
    18541 agaagaagaa acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa
    18601 cgaaatccat caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg
    18661 gaatgacgga gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg
    18721 acttgcgttg ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga
    18781 aacaactaat tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg
    18841 ctaatcaaaa gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg
    18901 gtcatttatt aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa
    18961 tggacacgtt gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac
    19021 atcaattaaa agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg
    19081 cgacagatgc cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt
    19141 tcaataaacc agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg
    19201 gattcaagca actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc
    19261 gggcaaaagc ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg
    19321 cggataattt atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag
    19381 aaaagaaaat cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa
    19441 aagaacaagc caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac
    19501 aaaaacttgt tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat
    19561 taaaaacgga atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc
    19621 aacgctatgg ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc
    19681 ataagatcgt tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta
    19741 cgatgaaaga acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga
    19801 aaaacggagc catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc
    19861 atgcactttt gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa
    19921 aaatctatca aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca
    19981 gccaaacaaa agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg
    20041 tcgtccaagc aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa
    20101 aaggaatgag aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac
    20161 cattgtcaga agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta
    20221 cgcgtgcaga gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag
    20281 gactgcaagt atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa
    20341 caccagattt gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac
    20401 aagcaggaac cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca
    20461 aatcaagcaa acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg
    20521 gggccaatct gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag
    20581 attgcaacta ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc
    20641 accggaaaat ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg
    20701 gaacgacaga aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag
    20761 aacgacgctt gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag
    20821 cagcactaaa agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg
    20881 tcagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt
    20941 cggcttattt ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga
    21001 caagactttt gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat
    21061 tattggtgag gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca
    21121 ggacgctgtt tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat
    21181 aacaattgga ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta
    21241 ggatcaccaa acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca
    21301 caagacaaag cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata
    21361 ccatcaatcg agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt
    21421 tcgcggtttt cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt
    21481 gaaaatcggt tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag
    21541 cggaaatcac gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag
    21601 aattttttcg caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg
    21661 ccgacacaat cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc
    21721 ggatcttgca gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca
    21781 gcgtacagag ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga
    21841 taagctgagg ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata
    21901 aacaaatatt cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga
    21961 acagtttttt cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt
    22021 tcaatcgtat gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt
    22081 taaaatttga ttttacgtaa aagaacaggt attctttttg taaactaacg tatttagttt
    22141 accatgtttc ctcggtttat caaactttta agaacaattt ttattagttg gcaagtagtt
    22201 ttggatagag aaaaaggagg aagttccagt ctattgacta ggagcaaaca aaatgaaaat
    22261 acacacacct atcggctttt tatggttaga tgcagatgaa tccggattga ctgctgtatc
    22321 ttttcagcca atttcagaaa gcgaatcagc gaatcagcga attttaacgc gcacacaaga
    22381 acagtttgaa gaatattttg cgggaaaaag aaaagaattt gatgttcctt tatctataaa
    22441 gagaggtacg ccttttcagc aaaatgtctg gaaagctttg tcgaccattc cttatggaga
    22501 aatccgaacg tataaagaag tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat
    22561 tgggcaggca aatcgaacca atccattgcc aatcgttatt ccttgtcatc gagtgatcgg
    22621 gcaaaatgga aaactgacag gatatatggg aaacgcaata gatggggttg aaatcaaacg
    22681 ccaattgttg ttgttagaag gatatctgca aaattaaaaa cagttgtgat tcggacattg
    22741 ttccatgcga gaatcacaac tgttttttca tgttcattct aaactttgat cagccaaata
    22801 aatagtaaag ccatcactgt ttggagaagc ccgacagcta atccataaga taagaagcgt
    22861 gtaccttctt tcatgaattt tgcaaagtct aaacgcagac caattgccgc taaagcagtg
    22921 atctcaaacc agctgctgat aaaatgtgcc gattggtcaa taactacagg taaagaaaac
    22981 aagctgttga ccaaacaaaa gatcacaaac cctaatacat accaaggaag cggtaatttt
    23041 cgttttttct gcgtatcagt tggcgtttca aacagagctg gttgtttctt gtgcatgaat
    23101 ttttcaaaca gatagacaac aacaacgagc atcataatac gtagaatctt aaatagcatg
    23161 gcgaattcga caacagaatt gtcaagtaag cttgccccag cgactacttg accaacagat
    23221 tggagtgtac cacccaaaag tgcacttttt tcaatgagat ttccaccaaa caactgtaat
    23281 ccaagaaaag ggagagtcaa catcatcaca gtacccagta gattgactag tgtgatgatc
    23341 tgtccttttt cttcctcttt cgcatggatg gaaggggcga ttgaagcaat agcagaagaa
    23401 ccacaaacag cgtttcctcc agccatcatc aaagaagaag tatcgctgaa ttgcagtttt
    23461 ttaccaatca agtaagcacc gatgatagtg actgacattt gcagtaacac aaaaacaatt
    23521 ccttttacac caatttgagc aatcgtttga gctgtgacag ttgcacctag taaaaaaaca
    23581 gagaattcta atagtttgct ttcagcaaat tttgtgccga ttgctagaac aggctgttta
    23641 aaaaaagtat ttcctaataa aatacccaat aaaatagcaa tagtcgctcc acctaactgt
    23701 ggcagataaa tagcaagaag cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg
    23761 ggtaagattt cgcggaccgc tgaatccttt acagatttta ttggatgtga caatgatttc
    23821 atttgatcgg ttcctttcgt caataaatac taaaagcata ctcgaacata gtagcactaa
    23881 accagtattt tgacaaaaga acaagaggtc gtgacaaaag tcttgtacga cctcttgttt
    23941 cgaataaacg gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaatt
    24001 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt cttttacgac
    24061 ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagcccaac cagtacaaaa
    24121 acatgaacag ctgtttttgt ttggccgttc ttattacttg atgcagaaca gctttttcac
    24181 aacctattga atcgattatt aagcgatttt tttagaaaca aattttccat gtttgctatg
    24241 ttctttaatt aagaaacgta cgtaatgagc aagaacgtag aagacaacta cacttgcaaa
    24301 cagacattcc gcaactttta agaaatccaa tacgctacta tagataccta aactgttgaa
    24361 gtaatgaaca gtagtaaata tagctgttgc agagataaca agcgggaatg taaaggctgc
    24421 atagcttgga taaaaacgaa gcttgaacaa acgtggaagc atagacagca caacgaaata
    24481 caatacttga gataaagcga acaatgctac gacaagataa aaattaggtt cagcaaagtt
    24541 tttaagataa cctgttaggc aaagagaacc aggtgctgca acgatcgtaa tcagcggacg
    24601 tgtcggatga gccatatttc ttacgaaaaa gacacgtttg atgatgatcg gcaataagaa
    24661 cgcatagcag atcaatgaaa tccaaaagaa aatttttcca ataagtggga agaagttccc
    24721 ggcagttaca gtgatgaccc caaatccaac gtaaatgata aaccagcttg gataaattgc
    24781 ttcaattgaa agatcagctt tgataacatg gtgataattg aaataaaaga caaggatcac
    24841 ttggaagata acagcgcaca gccaaatgaa tttgacaatt ggtgcaatac tttcatatga
    24901 aacaaaataa gtacaaataa ccatgagtga catcgtaaaa gttggtgaaa cggaggcgat
    24961 gattggatca tgcaacgttt gtttcgtgtg ttcgaataag ataaacaatt taacaaaaat
    25021 gaatatcata agaatcatag agataccgcc agttagattg cctaaagcaa catggtgata
    25081 gtctttaaac agatttccaa gggaagctaa cccaaggatc aacccgcaga tcgggatcgg
    25141 aataattttc aaaaagttct tcatatataa tattctcctt tttatttaat agattttagt
    25201 accaagatga acgaatacat tgtaaaatga ataggaatgt atgattacga gcaagcagac
    25261 agctgattgt cttcatctaa gttactagtt ggatcagcaa ctgttttgtt gtacatttct
    25321 attatagatg gttttttgta tatttaaatc aaatagtttt tatagtaatt atcataaaaa
    25381 gttatggaag tggtgccatg tttaagttat taacgacttt tcgagcagta tatgaaacaa
    25441 gaaatttttc cagagcagca gaagtactgt ttttatctca accagcagtc tcgaatcaaa
    25501 tcaaacagtt ggagcgggaa ttgaatatcg atctttttca acgtaacgga agaaaagaga
    25561 tgcttccaac taagcaagcc gacttattat atgaacggac actggttttg ttagaagaat
    25621 gggaagaaac tcagcaaaaa ttgctcaatg aacaaaatga agaagaagta tgtcggatcg
    25681 ttgcttccca cacctttgct gtctatcttt tgcctcagtt gatgaaacat ctgatcaaaa
    25741 ggtttcctaa agtgcatttt gagatcgaaa tcacgaattc tcatgaatca ttagaacaag
    25801 tagcgaaaca tgaatccgat tttggtttta tcgaaaagcc attagagact tccggcgtac
    25861 aaagatactc tttgatggaa gaccagctag tcatcgctgg ggacccaaaa agcaaattgt
    25921 ggcttgtaag agaagcaaac tctggtgttt ttcattacac gcagcgttat tttgaagaga
    25981 tgaatatcca agataagaag atgatcgtga aaagtaacga tgtaattgtt gcgttgctga
    26041 aggaaggaat cggtcgctcg attttgtcta agcgctctgt tccagaaaat gtgccaatga
    26101 ctccgttgaa tttccatcga tatttttatt tcatccaacg aaccaatctg gtttctgatc
    26161 agctgctaga gattgccgaa gagatccttc gatacaatga aagcacagaa acttctcaca
    26221 gcgaggagaa tctcagctgg tagataattg catgtatcaa aaaaagttct atgatacact
    26281 aaagataagc agtaggcttt tgtactgcat ttgctcaagc aactgcacaa acggaaagtt
    26341 aatttttccc aaatgtctat ggaaagactt tccattatct aactggaccg ctataataat
    26401 cagcagtatg gtttttaaga aaagggatga aacatggaaa aaaaattaaa agcttcgaac
    26461 gataatcgta ttgattatgg cgtgatttta ccagtctttc tgttatgttt gattggtctg
    26521 ctctcattat atgtagcact tagccatgat ccgaacaatc cagcagtctt gaaaggcgta
    26581 gggatgcaag ttgtctggta tttagttgga gcggcagcaa tcgttgtcat catgcatttg
    26641 aattccaaat ggatctggaa actgactcct tacctttatg gtttggggtt agtggtaatg
    26701 ggactcttgc tcaaattcta tgatcagaat ttagctgctt caacagggtc taaaaactgg
    26761 tttcgattcg gtgcgtttac tttccagcca gcagagttga tgaagattgc gtacatcttg
    26821 atgatggcac tgatcgtaac taaacataat acgcaagtaa aagaacggac gatgaaatcg
    26881 gacttttggc tgattgggaa gctcttgatc gtgacaatac cggttttagc attgatcatg
    26941 gcccaagatg actttggaac gatgcttgtc tttcttgcga tctttggcgg gattttcctg
    27001 atgtctggaa tctcttggcg catcatcgca cctgtagtaa tcttggcagt agtcgttgga
    27061 gcaggaacga tcttcttggt tacgactgaa ggcggaagag acttgcttta taaagttgga
    27121 ttcaaatctt atcagtttgc acgtattgat tcatggctgg accctttcca tgatacgtca
    27181 ggaatgagtt atcagccggc ccaaggactt ttggcaattg gtaccggtgg cttgtttggt
    27241 aaaggattca acgtaagcaa tatctatgtg cctgttcgtg agtccgatat gatttttacc
    27301 gttatcggtg aaaacttcgg ctttatcggt ggtgcattcg tcattttctt gtattttatt
    27361 ttaatctatc gtatgattcg ggtgtgtttc gatacgaata atgaatttta cgcctatatc
    27421 gcaagcggtt tgatcatgat gctgctgttc catgtctttg agaatattgg agcaaatatc
    27481 ggattacttc ctttaacagg gatcccattg cctttcatta gtcagggagg atcctcgatt
    27541 ttagggaaca tgatcggtat cgggttgata ttatcgatga gataccaaaa tgaagcacct
    27601 gcaagatctt caaggaggta agaaaattgt acacctttta ctggtacccg aaatgttcaa
    27661 catgcaaaaa agccaaagcg tggttagacc agcatggcgt tagttatcaa accgttgata
    27721 tgatcaaaga agtaccgaag gcctcaacga ttgagacttg gctagaaacg agtcaagtac
    27781 caatccgtcg cctgttcaac acaagcggga tgaaatatcg ggaacttgga ctgaaagatc
    27841 aagtaggcga gtttacggct tcaaaagcga gccaagtctt atcgacagat ggtatgctga
    27901 tcaagcgacc actgatctta cgagaggacc agcttgttgc aattggattc aatgaaaaaa
    27961 tttatgaagg agaactgata taaaatggca ggcaagcatt tgaagaaaaa ggacaacctt
    28021 tggatcttat ttaatggaaa agaatactgt gttggtttga caaatgaagc gcaagaagaa
    28081 cttggagaca tcacgtttgc caatctgcca aaagcaggac aatcgctaaa aaaagatgaa
    28141 cctctgatcg aagttgaagc ggaaaaagcg gtcaatgaat ttgccagccc gctaacgggt
    28201 acagtttctt ctgttaatga taaaatcggt gaagatatca atatcttgaa tgattcagat
    28261 gaaatgaatg cgtggatctt aagtttcaaa gatgttgatc caaaagaatt tgacgaatta
    28321 taaaatcaga aaatcacttg aaaaaagttg acactgattc ttccagttgc tataatcata
    28381 agcaatccta tatgaaacga gaaagctttg aaaagaagag tacatgtcac ggacgtttga
    28441 agagagccct gtttgctgaa aatgggtaac gaaaatgaga tggaagatgg tcttagagca
    28501 gagtaagcga gcgtaagcaa acttactacg ggagtgcccg ttacagcgcg acagtatacg
    28561 gaaagaagct aagtccaagt actggaagag gctgttattg cgaagtaacg gtaaatcaag
    28621 gtggtaacac ggaagttcaa gctttcgtcc ttttgcttaa ggcaagaggg cgaaggcttt
    28681 tttgtttgaa aaattataag agaaagatgt gaatggcatg tcattaatcc aactaacgaa
    28741 tgtcaaaaag acattcaatg ggaaaaacgg aaacatccaa gccgtcaatg acgtttcact
    28801 aaacgttgaa aaaggggata tttatggaat cgtcggctat tctggcgcag gaaaaagtac
    28861 attagtccgt ctcctgaatg gacttgagct tcccacgagc ggcgaagtga tcgtaaacga
    28921 tcaagatatt acaaaattaa aaaacaaaga attacgtaca ttccgaaaga aaatcggtat
    28981 gatcttccag catttcaacc tgttatggtc aagaactgta cttgaaaata tccagctgcc
    29041 gctggaatta gctggcgtac caaagagcaa acgtaaagaa cgggcagaag aactgttgcg
    29101 tttagtcggg ttagaaggac gcggacaagc atatccttca caactttccg gcggacagaa
    29161 acaacgcgtc ggtatcgcac gagcattggc aaatgatcct gagatcctgc tttgtgatga
    29221 ggctactagc gcattggacc cgcaaacaac agaagaagta ttagacttat tgttagcgat
    29281 caataagaaa ttgaatttga ccatcgtctt gatcacacat gaaatgaatg tgatccgcaa
    29341 aatctgcaac aaagtcgctg tgatggagtt ggggaaagtc gtagaagaag gagacgtatt
    29401 gactgtcttc agacaaccga aaaaagaagt aaccaaacgc tttgtacaac aagacatcga
    29461 accagaagct gattcgacag aactattagc tgaactcatc aaagaaaatc caagcggccg
    29521 tttggcaact ttgaccttta gcgaaagaaa tgccaacgag ccagtcattt cccaagcgat
    29581 tcgacgctat aacatcgatg tcaacgttgt gtatggaaag atcaaacaag tgaaagaagg
    29641 ttcctttggt tcattgactg tcttgatgac tggtgaaacc aaagaattag atcaagcaga
    29701 agtgttcatc aaagaacaag gagtaggaat tgaggtgatc catcgtggat aaaagctttt
    29761 cagaaacata tctagatttt aaacaaatca acccggaaac gatgaatcag gctatccttg
    29821 atacactata tatgacatta atctcaatgg tcttagtgac gatcatcgga ttagttttgg
    29881 gattgatcct ttactctatt ggacgcaaaa acaaaccgta tgctcgaatc atttatggaa
    29941 tcgtatcgat catcagtaat attttccggt caacgccatt tatgatcctg atggttttga
    30001 tcattccatt tactaaagca atcgtaggca ctatgctagg tgcgcaagca gccattccag
    30061 ctcttgtttt atccgcagca ccattttatg cccgtctagt agagattgct tttcgtgaag
    30121 tcagccctgg tgtactggaa gcagcagatg caatgggagc aagttactgg gaaatcatct
    30181 ggaaggtctt gatacctgaa agtattcccg cattgatttc tggattaaca gttacaacga
    30241 tctcgatgat cggatttact gcgatggccg gcgcgatcgg cgcaggtgga cttggaggtc
    30301 ttgcttggca ggaaggctac cagcgtggta atctaacagt gacattcgtt gcgactttaa
    30361 ttattttact aatcgtattt atcgttcaag gaatcggtga tttcttaact aagaaaacag
    30421 acaaaagata ataggatata gggggatgta ggatatgaaa aagaaaattt ttggatttgc
    30481 agcagcgctt ttgctgactg taggattagc agcatgcggt aacaacagcg acagcaaaga
    30541 ttcagcaaat aaagcagacg acacgacatt gaaagtcggc gcttcaccga caccgcacgc
    30601 agagattttg gaacatgtga aaccattatt gaaagatgaa ggaatcgatc ttgaaatcgt
    30661 caaattcgat gactatgtat taccgaacca atctttggaa gaaggcgaca ttgatgccaa
    30721 ttacttccag catattcctt atttgaacaa agaaatcaaa gaaaaaggat atgactttgt
    30781 caatgccggt gctgtccaca tcgaaccaat gggcttgtac tctaaaaaag tcaaagatgt
    30841 ttccgaatta aaagatggtg caacaatcat tacttcaaat tctgaatcag actggggacg
    30901 catcatcaca attctacaag atgctggctt agtcaaagtc aaagatggag tagatctgga
    30961 aacagcaaca ttcgatgata ttgaagaaaa ccctaagaac ttgaagttca accatacgat
    31021 cgacccatca cttttagctt ctacgtacca aaatgatgaa ggtgacctag ttgcaatcaa
    31081 cgcaaacttc gcttacggcg cagggttgaa tccattagaa gatgccgtat tgcttgaaaa
    31141 ggacaactct ccttacgtga atattattgc gactcgtaaa ggtgatgaag acagtgacaa
    31201 aatcaaaaaa ctgattgaag tgcttcaccg caaagaagtc caagaatgga tcacagacaa
    31261 atggggcggc tctgttaaac cagtaaaggc tgatgcaaaa taaggtatgg aacaaaaaag
    31321 atgagaaata agtctgtaat ggcttatttc tcatcttttt tatacctatt ctaatttaga
    31381 aatcaaaatt gtttaataat aaataaaaga tatttgattt tagtaaggaa ggtcattgcg
    31441 cttcattctc aattagtcta aaatgagtga gaacgaaaga gaatttgggg gtataatgca
    31501 tgtctgtctt agaaataaaa aatctacatg tgtcgatcga ggaaaaagaa atccttaaag
    31561 gcgtaaacct aacaatgaaa accggagaaa tccatgccat catgggacct aacggcacag
    31621 gaaaatctac cttatctgcc gctatcatgg gcaatccaaa ctatgaagta acagaaggcg
    31681 aaattttgtt tgacgggaaa aatgttttag acttggaagt agatgaacgt gcgcgacttg
    31741 gcttattttt agctatgcaa tatccaagtg agataccagg tatcacgaat gcggaattca
    31801 tgcgtgcagc gatcaatgcg aaacgtgatg aaaacgacaa aatttctgtt atggcatttt
    31861 tgaaaaagtt agatgaaaaa atggctttac taaatatgcc agaagaaatg gctgaacgtt
    31921 atctgaacga aggtttttct ggcggagaaa agaaacggaa tgagatcctg caattgttga
    31981 tgttagaacc tacatttgca attttagacg aaatcgattc aggtctggat atcgatgctt
    32041 tgaaagtcgt tgctaaagga gtaaatgaaa tgcgcggaga caatttcggt gcattgatca
    32101 tcacccacta tcaacgatta ttgaactata tcacacctga tgtcgttcac atcatgatgg
    32161 aaggccgagt agtcatgaca gggaatgctg acctagctaa acgtctggaa gcagaaggct
    32221 acgctggtat cagtaaagaa ttaggaatcg actataaaga agaagaagcg taagggggag
    32281 caagaatgaa agaaaaaaac tggctcgatt atcttgacgc ggtaaatgat ttttctttaa
    32341 gtaaaggcga acctgattgg atgagaacct tccgtcaaga cgcactagcg aaagcagatg
    32401 aactgccatt gccgcatatc gatcgggtga aattccatcg ctggtcgtta tttgatgtga
    32461 aagaaacaca aaccatcagt gaaacaggca cgattccggc atttgatgca atgaaagaca
    32521 atcctgtgct tgtccaacaa ggttcatgga cgatttttga acaactgcct gtggaattag
    32581 cagaaaaagg agtcattttt accgacttgt tcactgcaat gatcgaatat ccagaactag
    32641 tccaagaata ttatatgaaa aaagcagtaa acatgaacga agaccaattg actgcactcc
    32701 atgtagcttt tatgaacagc ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg
    32761 aaccactcga gtccttattc attcaagatg gcgcatccga tgagcacttc ttcaagcatg
    32821 tgttgatcgt ggcagatgaa catagtgaat tttcttatct ggaacgtttc cagacaacca
    32881 aagaacaagt agcaaaatca tcaggtaata tcatcgtgga agtcatcgct aaagctggtt
    32941 caaaaatcaa atattcagcc gtagatcaat taggagaaaa tattacttct tatatgaatc
    33001 gccgtgggca cattttacga gatgcttctg tcgactgggc aattggtgtc atgaatgacg
    33061 gtcatgtgat cgctgacttt gactcagatt tagcaggaga aggtgcacac gcagaagtca
    33121 aaatcgtggc aatcagctcg ggcagacaaa tccaaggaat cgatacacga gtaaccaata
    33181 aagcccctca tacgatcggg catattttgc aacacggagt gatccgagaa aaaggaacat
    33241 tgacctttaa tggtatcggc cacatcttga aaggggcaaa aggtgcagat gcccaacaag
    33301 aaagtcgtgt cttgatgctt tctgataaag cacgtggtga tgcgaatcct attcttttga
    33361 tcgatgaaaa cgaagtaact gctggacatg ctgccagtgt aggacgagtt gatccagaag
    33421 aaatgtacta tctgatgagt agaggattgc ataaagaaga agcagagcgc ttagtgattc
    33481 gaggattcct cggttctgtc ttaacagcaa tccctgtaga acaagttcgg aaagaattag
    33541 tcgaagtaat cgaagggaag ttgaatggat gatcgatgct aaaaagctac gcaatgattt
    33601 tcctatctta tttcaagacg tcaatgatga acctttagtt tatctggata atgcagcgac
    33661 gacccaaaag ccaaaacaag tactccaggc attagcgtac tattacgaac acgataacgc
    33721 caatgttcat cgcggtgtcc atacgttagc agaacgcgcg acccatgatt atgaacaagc
    33781 aagagaaaaa gtacgggcgt tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg
    33841 tggtacgacg acaagcttga attgggtagc cagaaccttc ggcgaccgct atatcaaagc
    33901 aggagacgag atagtgctct cttacatgga acatcattct aatgttatcc cgtggcagca
    33961 gttggcaaaa cgaacaggtg ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct
    34021 ggatatggag gatgcagcga aaaagattac ggacaaaacc aaaatcgtct ccattgctca
    34081 tgtatccaat gtcttaggtg tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga
    34141 aaacggcggg gtcatcgtag tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt
    34201 ccaagcacta gactgtgatt tctttgcttt cagcggacac aaaatgtgcg gaccaacagg
    34261 tattggtgtt ttatatggta aacgaaaatg gttagaagaa atggaacctg ttgaatttgg
    34321 tggcgaaatg atcgattttg tcgaattata cgacagtaca tggaaagaac taccttggaa
    34381 attcgaagca gggacaccaa atatcgccgg cgctatcgca ttaggccatg caatcgatta
    34441 tttgcaaaaa ctcggtatgg acaatatcca tcgatatgaa gaagaactag ccgcttatgt
    34501 gttgccaaaa ttacaagcaa ttgatggatt gactgtttat ggtccacaag acccgaattt
    34561 tcggacagga gtcgttgcat tcaatttgga tgggttacat ccccatgatg tcgcgacagc
    34621 tttagacatg gaaggggtag ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa
    34681 gtaccttgaa gtatcagcga ctgctcgcgc gagcttttat ttctacaaca caacgcaaga
    34741 cgccgaccgt ttagttgaag caatcatggc aacaaaggag tttttccaac atgggactat
    34801 ctaaattaga caatttatac cgtcaagtga tcttggatca ttcttcccat ccgcatcatc
    34861 gtggaagctt gactgactcg acaagcaaga tcgaattgaa caaccccaca tgtggagatg
    34921 tgatcgaatt gcaagtcgat gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca
    34981 gtggctgctc catcagtacg gcaagtgcct cgatgatgac agacgccgtc atcggaaaat
    35041 caatagaaga agcagaacat ttaactgtag actttctaca gcttgtccaa ggaaaagaag
    35101 tcaaagatcc cgaccaatta ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg
    35161 ctcggatcaa atgtgcgaca cttgcctgga aagcactagg aaaagcaatg gaaaacgatg
    35221 gtaccgcagc tttccatcat taccacaagg aagaggagtg agaacatggg cgtaccagaa
    35281 ttagaagaat ataaattcgg gtttcacgat gatgtcgaac cggttttcag tacaggagaa
    35341 ggcttgacag aagaagtgat ccgtgagatg tcaagaatca aaggcgaacc agaatggatg
    35401 cttgaattcc gtctgaaatc tctagaaaca tttaacaaaa tgccaatgca aacatggggc
    35461 cccgaccttt cagacatcga tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa
    35521 cccgctcgtg actgggaaga cgtaccagaa aaaatcaaag atacatttga aaagattggt
    35581 ataccagaag ctgaacgagc ttatctagcc ggtgcttccg cacaatatga atcagaagtc
    35641 gtgtaccata atatgaaaga tgaattcgaa aagttaggaa tcgtctttac tgacactgac
    35701 tctgctttga aagaataccc tgaattattc aaagagtact tttcaaaact tgtaccgcca
    35761 accgacaaca aattagcagc gctgaattca gccgtatggt caggtgggac ctttatttac
    35821 gtgccaaaag gcgtcaaagt cgatgttcca ttacaaacgt acttccggat caatgcggaa
    35881 aacacggggc aattcgaacg tacgctgatc attgttgacg aaggagcaag catccactat
    35941 gtcgaaggtt gtacggcacc tacttattca agtaacagcc tgcatgccgc catcgtagaa
    36001 atctttacaa gaaaagatgc gtattgccgt tacacaacga tccaaaactg gtcagacaat
    36061 gtgtacaact tagtaaccaa acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc
    36121 gacggaaacc taggtgcaaa aacaacgatg aaatacccaa gtgtttactt agatggaaaa
    36181 ggtgcacgag gcaccatgct ctccatcgcc tttgcaggag caaaccaaat ccaagatacg
    36241 ggtgcaaaaa tgatccacaa tgcaccaaat acatctagct ctatcgtctc taaatcaatt
    36301 tcaaaagacg gcggagaagt aaactatcga ggacaagtaa cctttgccaa aaacagtgcg
    36361 ggttcgatct ctcatatcga atgtgacacg atcatcatgg acgaatggtc gaaatccgat
    36421 acgatcccat tcaatgagat ccacaacagc caagtctctt tagaacacga agccaaagtc
    36481 tcaaaaatct cagaagaaca actctactat ctgatgagcc gaggcctgac cgaatctgaa
    36541 gcaactgaaa tgatcgtcat gggctttgtt gaaccattca ccaaagaact tccaatggaa
    36601 tatgcagtcg aactcaatcg attgatcagt tatgagatgg aaggcagtgt tgggtaaacc
    36661 tgttatagga gctagaaccc ttgatata
//
LOCUS       DAZKPO010000020        35443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000020 DAZKPO010000000
VERSION     DAZKPO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.020"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..1393)
                     /locus_tag="ACG8NZ_000979"
     CDS             complement(125..1393)
                     /locus_tag="ACG8NZ_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0538674.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1624..2361)
                     /locus_tag="ACG8NZ_000980"
     CDS             complement(1624..2361)
                     /locus_tag="ACG8NZ_000980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0538675.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2428..2997)
                     /locus_tag="ACG8NZ_000981"
     CDS             complement(2428..2997)
                     /locus_tag="ACG8NZ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0538676.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3125..3997
                     /locus_tag="ACG8NZ_000982"
     CDS             3125..3997
                     /locus_tag="ACG8NZ_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0538677.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4339..5211
                     /locus_tag="ACG8NZ_000983"
     CDS             4339..5211
                     /locus_tag="ACG8NZ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0538678.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5208..7166
                     /gene="nagE"
                     /locus_tag="ACG8NZ_000984"
     CDS             5208..7166
                     /gene="nagE"
                     /locus_tag="ACG8NZ_000984"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0538679.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7498..8349)
                     /locus_tag="ACG8NZ_000985"
     CDS             complement(7498..8349)
                     /locus_tag="ACG8NZ_000985"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0538680.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8472..8939
                     /locus_tag="ACG8NZ_000986"
     CDS             8472..8939
                     /locus_tag="ACG8NZ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0538681.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9202..9684)
                     /locus_tag="ACG8NZ_000987"
     CDS             complement(9202..9684)
                     /locus_tag="ACG8NZ_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0538682.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(9784..10647)
                     /locus_tag="ACG8NZ_000988"
     CDS             complement(9784..10647)
                     /locus_tag="ACG8NZ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0538683.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            11245..12120
                     /locus_tag="ACG8NZ_000989"
     CDS             11245..12120
                     /locus_tag="ACG8NZ_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0538684.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(12265..12417)
                     /locus_tag="ACG8NZ_000990"
                     /pseudo
     CDS             complement(12265..12417)
                     /locus_tag="ACG8NZ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(12685..14016)
                     /locus_tag="ACG8NZ_000991"
     CDS             complement(12685..14016)
                     /locus_tag="ACG8NZ_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0538685.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(14165..14878)
                     /locus_tag="ACG8NZ_000992"
     CDS             complement(14165..14878)
                     /locus_tag="ACG8NZ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0538686.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            15053..16477
                     /locus_tag="ACG8NZ_000993"
     CDS             15053..16477
                     /locus_tag="ACG8NZ_000993"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0538687.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            16776..17483
                     /locus_tag="ACG8NZ_000994"
     CDS             16776..17483
                     /locus_tag="ACG8NZ_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0538688.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            17470..17793
                     /locus_tag="ACG8NZ_000995"
     CDS             17470..17793
                     /locus_tag="ACG8NZ_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0538689.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(17882..18919)
                     /locus_tag="ACG8NZ_000996"
     CDS             complement(17882..18919)
                     /locus_tag="ACG8NZ_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0538690.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(19240..20223)
                     /locus_tag="ACG8NZ_000997"
     CDS             complement(19240..20223)
                     /locus_tag="ACG8NZ_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0538691.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(20638..21408)
                     /locus_tag="ACG8NZ_000998"
     CDS             complement(20638..21408)
                     /locus_tag="ACG8NZ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538692.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(21401..22336)
                     /locus_tag="ACG8NZ_000999"
     CDS             complement(21401..22336)
                     /locus_tag="ACG8NZ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538693.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(22652..24301)
                     /locus_tag="ACG8NZ_001000"
     CDS             complement(22652..24301)
                     /locus_tag="ACG8NZ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0538694.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(24418..27021)
                     /locus_tag="ACG8NZ_001001"
     CDS             complement(24418..27021)
                     /locus_tag="ACG8NZ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0538695.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(27033..28082)
                     /locus_tag="ACG8NZ_001002"
     CDS             complement(27033..28082)
                     /locus_tag="ACG8NZ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0538696.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(28108..28422)
                     /locus_tag="ACG8NZ_001003"
     CDS             complement(28108..28422)
                     /locus_tag="ACG8NZ_001003"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0538697.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(28438..28884)
                     /locus_tag="ACG8NZ_001004"
     CDS             complement(28438..28884)
                     /locus_tag="ACG8NZ_001004"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0538698.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(28885..30807)
                     /locus_tag="ACG8NZ_001005"
     CDS             complement(28885..30807)
                     /locus_tag="ACG8NZ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0538699.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(31019..31915)
                     /locus_tag="ACG8NZ_001006"
     CDS             complement(31019..31915)
                     /locus_tag="ACG8NZ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0538700.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(31908..32588)
                     /locus_tag="ACG8NZ_001007"
     CDS             complement(31908..32588)
                     /locus_tag="ACG8NZ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0538701.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(32589..33371)
                     /locus_tag="ACG8NZ_001008"
     CDS             complement(32589..33371)
                     /locus_tag="ACG8NZ_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0538702.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(33364..34281)
                     /locus_tag="ACG8NZ_001009"
     CDS             complement(33364..34281)
                     /locus_tag="ACG8NZ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0538703.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(34445..34699)
                     /locus_tag="ACG8NZ_001010"
     CDS             complement(34445..34699)
                     /locus_tag="ACG8NZ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538704.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT        10628 a         7193 c         6038 g        11584 t
ORIGIN      
        1 agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg
       61 aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct
      121 ctttttaata accataaaat cctattcctt catatctcca gcccatcggt gttagccaat
      181 ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata
      241 gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga
      301 tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag
      361 gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat
      421 attcccagcc tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt
      481 ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat
      541 actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag
      601 tggcataacg acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc
      661 atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt
      721 tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc
      781 attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt
      841 taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt
      901 caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt
      961 gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat
     1021 aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca
     1081 atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag
     1141 cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt
     1201 cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct gtatcgtttt
     1261 caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag
     1321 ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat
     1381 tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa
     1441 ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc tttttaataa
     1501 atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta
     1561 aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat
     1621 ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg
     1681 cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt
     1741 ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt
     1801 ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg
     1861 taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg
     1921 ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc
     1981 catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat
     2041 attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt
     2101 attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac
     2161 ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc
     2221 ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc
     2281 tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac
     2341 tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac
     2401 tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt
     2461 ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc
     2521 tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat
     2581 ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc
     2641 ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa
     2701 acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat
     2761 actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac
     2821 tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt
     2881 attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag
     2941 cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt
     3001 gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa
     3061 catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga
     3121 aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag
     3181 ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt
     3241 agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga
     3301 ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc
     3361 gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac
     3421 taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat
     3481 ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt
     3541 acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact
     3601 gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac
     3661 agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt
     3721 ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac
     3781 atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc
     3841 tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc
     3901 attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg
     3961 taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt
     4021 cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc
     4081 ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa
     4141 agaattgaca aaacataact gtctatataa aattgattac gttaacaata attcgtttca
     4201 tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt
     4261 tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc
     4321 ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta
     4381 gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga
     4441 aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa
     4501 atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg
     4561 atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct
     4621 ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac
     4681 aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca
     4741 gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc
     4801 gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa
     4861 gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac agatatccat
     4921 tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa
     4981 cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga
     5041 aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac
     5101 cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga
     5161 agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc
     5221 tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac
     5281 tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac
     5341 aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac
     5401 tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag
     5461 ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat
     5521 cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt gccggattga
     5581 ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct
     5641 ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag
     5701 tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt
     5761 tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta
     5821 caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata
     5881 ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat
     5941 ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac
     6001 gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct
     6061 ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg
     6121 tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag
     6181 caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg
     6241 cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg
     6301 gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc
     6361 tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca
     6421 aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac
     6481 gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg
     6541 gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag
     6601 tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac
     6661 aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt
     6721 acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg
     6781 aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa
     6841 tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg
     6901 ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat
     6961 tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg
     7021 atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg
     7081 gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa
     7141 tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa
     7201 caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt
     7261 ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt
     7321 atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg
     7381 ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt
     7441 cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta
     7501 ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac
     7561 tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc
     7621 taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc
     7681 tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc
     7741 taattcttca tagtcagtta gattgccgga taattctaag atttctggag agtcactttc
     7801 tactagtact tttgctgcag cttctgcata ttcacgtttc aatacccaac ctgtttttcc
     7861 atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc
     7921 attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat
     7981 tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa
     8041 acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg
     8101 ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa
     8161 cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc
     8221 tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac
     8281 catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa
     8341 ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt
     8401 ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag
     8461 gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata
     8521 ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa
     8581 caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta
     8641 caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag
     8701 atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc
     8761 caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc
     8821 agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag
     8881 ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat
     8941 tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc
     9001 cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg
     9061 aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa
     9121 aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct
     9181 actttcacga cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat
     9241 atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta
     9301 atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc
     9361 ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat
     9421 tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc
     9481 gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc
     9541 tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac
     9601 aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt
     9661 ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa gttcatctta
     9721 cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt
     9781 taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc
     9841 tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag
     9901 ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt
     9961 aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt
    10021 ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc
    10081 tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg
    10141 catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac
    10201 tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt
    10261 ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg
    10321 aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg
    10381 aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac
    10441 tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc
    10501 cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc
    10561 tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc
    10621 gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat
    10681 attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa
    10741 tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat
    10801 ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa
    10861 acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa
    10921 agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat
    10981 catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt
    11041 cagataaata aaaaacaaaa aaagttccat ctttaaaaca cgctattctt ttactttttc
    11101 tcacattctc taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt
    11161 atcaaatatg taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata
    11221 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
    11281 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
    11341 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
    11401 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
    11461 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
    11521 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
    11581 gagattctta tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat
    11641 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
    11701 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
    11761 gcagttgaat cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
    11821 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
    11881 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
    11941 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
    12001 cgctactcca taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
    12061 aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
    12121 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
    12181 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
    12241 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta
    12301 gattttccgg cattgttgat agattttatg atacttttct acattttgta agagaggttc
    12361 cactgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc
    12421 taatctttaa agataaatat taaaataacg attaataata ttaacagtct ttatttttca
    12481 ccaccaaaaa cctatctttg catatactaa atagacgtat atacacagat aggtttgtct
    12541 tgtgaaaaat aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta
    12601 tcaaaaaata tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt
    12661 taatttttgt tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg
    12721 ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg
    12781 taaaacagct gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt
    12841 agtccaagga atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc
    12901 tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac
    12961 aataggcaat ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa
    13021 tttagcaaag tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc
    13081 aatcgtattt cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat
    13141 atgtggaatt tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa
    13201 aaacggatct aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag
    13261 attagcaata gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg
    13321 ctctgtaact atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat
    13381 aaatgacaat aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga
    13441 ctttaaaaca gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga
    13501 aagtttaata aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga
    13561 agctcctgta taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc
    13621 aggagtaaac atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac
    13681 cgctgcaata tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac
    13741 aactaataat gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa
    13801 cgtggtcaat gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac
    13861 tgaattaata agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc
    13921 atctcgcaaa gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt
    13981 ttcaaataca atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg
    14041 taatcgatta ctttttccac ccaaataata catcacttat caaaaaaagt aaatactttt
    14101 aattaaatat atatatcttt tttagtataa aaaaataagc tctgaaaaaa taattttccc
    14161 gagcttattt ttcaacaaaa acagcttcaa aaacaaagtc ttgatataaa tgtctcgata
    14221 ccgaatactc gaaaggttta ccagacgata gagcaacaac tttgtctatc tcaatcataa
    14281 attcacctgg atctattttt aatatttttg cgtcaaattc attcgcagta tctcctttga
    14341 tacgtatgtg cgaagtcttt atttccagac ccagttcatt tgtgatataa gcatatacag
    14401 atttctttaa atgttttggt tccaagcctg gtatgactgc taatggcatg aatgtttgct
    14461 caatcgagta tggttgacaa tcaataaaac ggcttcgtac aatattatat acatagtctt
    14521 ttacactgat acttaattta tcagctactt ttttcgatgg aagttcaatt gagaaagtat
    14581 ggatttctga atctacgtgt tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa
    14641 gaactcgtga gttatcaatt ggtgttctga cgacttcagt cccataccca cttctacttc
    14701 gcaaaactcc ttcggaaaca agtaagtcta cgcctttctt tactgttaac ttgcttgtat
    14761 gataaataac tgccaattca tctcctgtag gaagtttctc accaatttta tactctccat
    14821 ttacaatttt tttctttata tctaagtaaa tttccatata cttcgcaatt tttttcaatg
    14881 gtgaccctcc aatcaaaaac atatcttcat ctatacttta acataaataa accgataaaa
    14941 tccacatcaa aattatacac acaataaaaa agatatatat atttaaacaa aactatttac
    15001 tttaagttgt aagcggtgtt atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac
    15061 tatgaaaaag ctaacttttc cagatgattt ttggtggggt tccgcttggt cagctgagca
    15121 agcagaagga aaaggagaaa caggaaaagc agagactatc tgggatcgct ggtttaaaga
    15181 acaaccttac cggttttatc aacgaatcga ttcctctatt acaaccgatc acatccatca
    15241 atataaagaa gatatagaac taatgaaaaa aactgggcat aactcctttc gcgtttctat
    15301 ttcttgggca cgaatgtttc caaatggcgg taccggaaaa gtaaatccca aagccatttc
    15361 tttctatcga aaattatttt ccgaaatgaa taataacgga atcaaagtat ttgccaactt
    15421 atatcatttt gacatgcctg ctactcttca agataaagga ggatgggaat cacgtgaagt
    15481 agttgatgct tatgttcatt acgctgaaac ttgttttaaa gaatttggtg acttagtttg
    15541 tcattggttt accttcaatg aaccacttgg gcctatttta ggtacttatc ttgaagattt
    15601 tcattatcca aacctcattg actttaaacg cggagcgcaa gcagcattct tcactatact
    15661 tgcacacgca aaagcaatac aagtatttaa agaactagct cttcctagta aaataggcgt
    15721 tattctaaat ttgagtccta cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc
    15781 agaaatcgct gatttatttt atacgcggag ctttttagat cctatggtta aagggaattt
    15841 ttctaaaaag cttatcctgt tattacaaga atataatcag cttcctgaag attatacgaa
    15901 agaagatctt gtatgtatct acgaaaatac cgctcaaatt cttggactaa actattacga
    15961 gccacgtcgc gtcaaatctc gttcttctat ggtggataca tctagtccat ttttaccaga
    16021 gtggtttttc gaaccacata tcatgcctgg acgacgaatg aacgaatacc ggggatggga
    16081 aatttacgaa aaaggaattt atgatttgtg catggatatc aagaaccatt atggaaacat
    16141 cgaagcattt atctcggaga acggaatggg cgttgcggat gaagaacgtt tcatggatga
    16201 tacagggcaa ataattgatg attatcggat tgagtatatc aaagatcatc ttgcttactt
    16261 atggaaggca ataaatgaag gatgtaacat taaggggtat catctttgga catttattga
    16321 ttgctggtca tggatcaacg cctataaaaa ccgatatggg ctggtttcac tcgaccttac
    16381 tactcaaaaa agaacaatta aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa
    16441 tggatttaga tacgatgtag acttgttagt tgaataatat tctaaaattc cacttgaagt
    16501 gaacacatta gaaaacaaga ataatgagaa ataccaataa caaatacttt ttcttcatac
    16561 ttcatttata cttgtcagta taagagactg tgacaaatgt agtgatcctc agaagttagg
    16621 tctggaaatc taacttgaga agattttttt aaactacgtc cagtcttttt tatttgttta
    16681 aaattgaagt tgacaagtct tcattaaaaa tttagaatat tcacaagttt gacgacaatt
    16741 atgaaaattt atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa
    16801 ctgcgatcaa agacacgtta cctaccgttt ttggttatat cggtattgga cttgcatttg
    16861 gtatcgttgg gaaagctgcc gggtttcatc cgctggtcgt cacgttgatg tccctgctga
    16921 tctatgctgg ttctgcccaa tttatcacgg tcagcatgct tgctagccat agtccactgc
    16981 tctccatcgt tctctcgacc tttctagtca attcccgaat gattctgatg agtatgacga
    17041 ttgctcctta tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga
    17101 cagatgaaag tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact
    17161 ttcgttggat gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg
    17221 ttggcgcact tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca
    17281 tcgtcgctat gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta
    17341 aacgattaca gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt
    17401 ttatcccaag taacttagtg attttagttg taacactgat tgggtgcgga ttgggggtgt
    17461 ggatcaaaca tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca
    17521 acttggcttt ctaggatcct accatttgtc ttactgaaaa aattcgattt gccgcaaccg
    17581 ttgcttgagt atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt
    17641 ctatttaccc aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca
    17701 gttcctacac ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggtagga
    17761 attctttccc tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat
    17821 tactgtcatg cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg
    17881 cttatccgtt gaacggacca ctaacaacga cttttcctaa tatatgtcct gtttctacgt
    17941 ctttggttgc ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg
    18001 tactgcggat aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg
    18061 tttcgatttc atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag
    18121 attggttttt ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat
    18181 gtccaaatgg tcggacaagg tgtttgattt gatcaaagta gcgggtaata tcgaataata
    18241 cagcgacata ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat
    18301 gataatcgat tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg
    18361 cagtcgcgta gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc
    18421 ccccagctcc gttgatcact aagatttttt tcccgcggtt ggcattttct tctggagtca
    18481 gaccaaattt ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcgg
    18541 cttcttcatc tgaaagattt tttggtgcaa gagcgacaat acgttcgtca accagttggt
    18601 attcttgatt gcttcctgct cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag
    18661 aaaatttttg cacttcatca cctacagcaa caactttccc gactccatca aaccctaaga
    18721 tacgtaaagc atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag
    18781 atactgcgtt gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt
    18841 catcaataaa gctttttgga tcatcgatcg acaacccttc ataaaaaccg actaccttca
    18901 ttaattcctt ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac
    18961 agttttcatt tcaatatata tagtatgcta tttgttccac ttttattcat cataattatt
    19021 ctcttactta aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact
    19081 tttgtcacaa cctcttttaa acggtgttca atcaactgac cctcggtatt cgctcaaaaa
    19141 acgacaaaat atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc
    19201 tttttcacga cctctaattc acggtgttca agcaagaaat cattgttcac tgaaaagcgt
    19261 tggatgttct ttttcaaagg tgtctaagat ctctcgtact tcatcagtag atttcgtatt
    19321 catcaactga actcgcaagt catttgctcc tggaaatcct ttgatataga ttttaaagaa
    19381 acgatgtagt cctgtgatgg aacgcgggat gatttctgaa taatgatctt gcagatctag
    19441 ttgcagacga agtagtccga tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa
    19501 tgcataagga ttcttgaaaa tcccccggcc aatcatgatg ccatctacgc catatttttc
    19561 ggccaattct tctcctgtct ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg
    19621 cggagcgatt tggtcacgta aagcaatgat tttagggatg acttcccaat gtgcatctac
    19681 tttactcatc tctttacgcg tacgcaaatg gacagaaaga ttggctatat cttgatgaag
    19741 taggtgactg atccattctt ccatctcaga catttccgta tagccgatac gcgtcttgac
    19801 gctgacaggc aaaccgccag ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg
    19861 aaggatcaag ccactgcctt ttccacgttc tgctacatta gggactgggc agcccatgtt
    19921 gatatcgatt ccttggaagc ccatttctgc catagcaatg ctcatttctc taaaaaattc
    19981 tggcttgtct ccccaaatat gggcaaccat cggctgttca tcttctgtaa aggtcaatct
    20041 tcctcgtaca ctgtcttttc catctggatg acagaaactg tctgagtttg taaactcagt
    20101 aaagaagaca tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa catctgttac
    20161 atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa aattagtagt
    20221 catttatttg ttactctcca ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat
    20281 tgtcattata ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag atgtttctga
    20341 ttgagcaaaa aaagactgtg acaaaaatct tatcacaatt ccttttccgg aataaatggt
    20401 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac gaaaaatagg aggagctatt
    20461 tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccactaaa
    20521 cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cgacaaaata tgaggaacta
    20581 ttttcccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttttcta
    20641 acttctcaat cctcttcctt tattgatcaa ataccaagta acggcgtaca aaacaacgat
    20701 gaatagcaca agtacgatca tcgatgttgc tactggcaca tcgattgttc caaggaatcc
    20761 gtaacggaaa ccggagatca tataaacgat cggattgact ttcgaaacag cttgccaaaa
    20821 tggcggcaac atagagatgg catagaaaac gccacctaga taagttaacg gctgcaagac
    20881 aaatgtaggc acgatcgata catcgtcata agactgagca aaaatcccgt tgagcagtcc
    20941 tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg atgaccatga accatgaata
    21001 cacatgaagt ggtacaaaga acaaggaaat gatcgttacg agagagccga cgagaatact
    21061 gcgacccact ccgccaatca caaagcccca aatgatcaca tgcgtaggaa caggcgctac
    21121 caagatttct tcgatgtttt tctggaattt ttgagagaag aaggacgaag aaacgttggc
    21181 atacgaacta gtgatagcag acatcataat caaaccaggc acgatgaatt ccatatatga
    21241 aaaaccaccc atgtcaccga tccgaccacc gatcattttt ccgaaaataa caaagtacaa
    21301 ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag atacgcaaat agcggttcgt
    21361 ttccttgata gctaaacttt ttaatgcagt aaaataaaga ttaaacattt ttctcctccg
    21421 tatgattatt ttcttctgtg attttcaaaa acagttcttc taaacgattc gacttattcc
    21481 tcatagaaag aactttgatt ccttgtcttg tcagctgatc gaagattccg ttcacccctt
    21541 gatttcgttc aacttcgaca gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt
    21601 caatcacagg tttggtctca tatggcgcta aatcaaagat aaatgtttca aattgcagtt
    21661 tggatagaag ctctttcata ctagtattct cgatcagttc acctgattgg atgatcccga
    21721 tgttgcgaca aagcatttca gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac
    21781 cattttcgtt caattctcgg agaaactccc acatctcgcg tctcaattca atatcgactc
    21841 ctgctgttgg ttcatccaag atcagcaaac gtggttcatg catcaatgcc ctagcaatca
    21901 tcagtcttcg cttcattccc ccggaaagca tacgtgcacg gacatttctc ttttcccaaa
    21961 gattggactg cttcaaatat ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt
    22021 agtagcctgc ttgattcacg acgatttgct ggaccgtttc aaagggattg aaattgaact
    22081 cttgcgggac taatccaatc tgttgctttg ctcggactag atccgtatcc agatcataac
    22141 cgaagacatt cacttttcca gatgttttat tgactaaaga agtaatgatt ccgatcgttg
    22201 tagacttccc agctccattt ggtcctaata gcgcatagaa atctccttct ttcacggaaa
    22261 gatcgattcc tcgcaatgct tcgaccccac ttccataaac ctttttcaaa ttatttattt
    22321 caagcgcatt tgccatttat ttcctatctc cttatagaca tgattcttta ttgtatcatg
    22381 gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa atcaggttgc tgggctcagg
    22441 aaaatctcct aatgaaagac atcaatcgga cgaagtcaga caaccttttc agaaaaaaca
    22501 aatcgaagat aaaggattac tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa
    22561 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcaaaa gccaagattt
    22621 ttgatccaat aaatactttc gatcacttcg cctatcccaa tacatgccac tgccgctttt
    22681 catcaaataa cgcggcttcc gtttttgcgg tttctactaa agtctctggg taatccaaaa
    22741 ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa
    22801 aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct
    22861 ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg
    22921 caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt gtccctttta
    22981 aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt
    23041 tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg
    23101 ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga
    23161 tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg
    23221 cattggaacc cgtgactaat gtattcttct gccagttgac cggattcact accgcttctt
    23281 ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc
    23341 aagtgatggg catataagta cggtaattca atattgcagc ggctacttct aattctccca
    23401 gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat
    23461 gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc aactctgctt
    23521 cactcccact cttcatacga aatgaaaaac ctaatttagg gatgtctctt aatggtttag
    23581 tttgctgttt cagctcttct tgaagtggtg tcggcagttt tgctagctga ttggcggctg
    23641 aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca
    23701 ctagataata gaccgtattg aatactaacc caccgattgc caaatagatt cctagtactt
    23761 gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa gccaagaaaa ataaataaac
    23821 ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat
    23881 ctttttttcc taaacgagcg aattgatatt ggattttttc tctcagttga ggattttcct
    23941 gataaaaagc aatcagtttt tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac
    24001 gttgatacaa cgcttctgaa cctacactag aataggtact gttgattcct tcgaatactt
    24061 cgaacatatc caggtcatac caagtaagat catcgatttc gctatcccat gaacggaact
    24121 tcttctccgt gagccatgct tccttcaagc tttcttcttt atcaaatctc ggttgataag
    24181 gaattttttc ccatttgcta cgaactgttt cctttaattt caaacgtgaa aaaatgtcta
    24241 aagcaactaa taaaataaca gctgcaataa taattaaaac gataaattct ctttgaccca
    24301 tatccctctc cgttccttta tttgttagct ttagtatagc actgttgcgc tatttgaaaa
    24361 gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt tcttgtatat tacatattta
    24421 tctctcattt atcaaaattg acaaataatc atagggagga attgttgtta attcttgttt
    24481 attctccagt gcattgacaa ctgtcccaag ggaaaggata gcctcaggta ctattttttc
    24541 ttgttctgtt ggattagcta agcgaagaac aaatccttgt tcatgaaaat aagaaggata
    24601 aatcgatgat acaattaagt cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc
    24661 aagttcattt tgtaatggcc aaatcttgtt atctaacctg tgaataaata cattcagttt
    24721 ctgcttttga taagaaatag attgttctaa ccgtttttct gcctgttttg ccactttgaa
    24781 ctcattaaat cttccttctt ctacattaaa tccaaaagtg actttatact cacctatctc
    24841 ttgagctagc ggtgtttcca tcatgacatg cccttcattt gtcgtatctc ctgaagctcg
    24901 cccaggtcgc caagcaagat taggttttcc aagttcccct gttgttgaaa ataaggttat
    24961 gattaattga ttttctatac gttcatactc ttttaaacca tctgcataaa acgtaaaata
    25021 atgatcattt tctgccacag agacactctt atcgaatatt tctaaattaa caggtttctc
    25081 taccatagtt tctgaccaat tcggaggaat gacatcaggg gtattttcaa taaatccgtt
    25141 ttgaatttgt gcaattgatt tttcagattt atctaaaaca ttaattgcta agcgcatacg
    25201 atgcgagtaa acagtattat gaattttgag tgtgccttca acgatttgac tttctcccag
    25261 actgaacgat atttcataag atatttcttc catttcgggt ttttcagata aacgatcttt
    25321 taaatcttta ggaagttgag cttttccgta aacaactaat gtttcttgta aagaatcttt
    25381 atatacttta gctgtttcta attttagaga tagttctgta tctccttcta atggtgaata
    25441 atcatacgta tcgccatcat ttgctgaatc gatcaaatga acgaaatcaa gatattgtct
    25501 atctccaact atcaaattca attgcccatc tttaaaagaa agtttatatt tattgttact
    25561 tatctcagtt ttgtctatct cgattgctgt ttctaatttt tcattatttt tctcaaattc
    25621 aacaacagtg tatcctagtg ctggcacatt tacatttatt tttatgtcaa gttcataata
    25681 agctggttca tcaaaatatt cgaaacctgt agcaatcatc cgataagcat ttttccgttc
    25741 tggaaaataa acttcttttt caatgatatt atagggacta cctttgaagt gtattttctt
    25801 tgaatcagct actacatgca cgattttcga tccactataa ttctcagtcg ttgtgttgaa
    25861 aacaaggact tcattcgatt ttagctttaa tgcatcagcc atccgtttta caataaggtt
    25921 ttcgattccg tcagctatct cattagcttc tttgattcga tggaaaatat cttccgcaac
    25981 attatctgaa acacagccac caatactatc atgagcctgg ttttgcaaaa tctttttcca
    26041 taaccgacga agtaggcctt tactgatatg gataccattt ctttctgcaa tcagcatgag
    26101 cggctcaact cgttttagaa ttttttgttc caatttaaaa ttttctattt tcatttttgt
    26161 tcttactgag ccaatagaac gatgaacacg agagtaaacc ggctgacgaa gttcaccttt
    26221 atattcaggt aattcttttt tgtttctgat aacttctaca taagaaggat aatctgaaat
    26281 aatattgtgg aatttactgt atttattcaa cttctctacg acttgatcaa aatttaaaat
    26341 cattgacttt tgatctacac ccgaaggtac taaaatatct ttgttttttc cttgcatatg
    26401 aagcatttca actgcttcat ccaacttttc ttttgcaaat tcaggaatat ctttaattgc
    26461 gtctaaggtc atcattccag tcatataacc aaatggaaaa ttcatggcat aaacaaattt
    26521 atctcccaat cctttccaga taaaataagg agatggaacc atcgtttcaa agtttaatcc
    26581 tcgccaacac atgaaattat cgatttctac ttgatgcaaa agtgtaggca aattcgcatt
    26641 gaacccaaac gtgtcaggca aataccctac catcattgga tctccatatt taaggatcgt
    26701 atcactgatt ccaatcatta agtttcttaa aattgattca gcatccacta ataaggcatc
    26761 tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt ttcatcagtc gttggataac
    26821 cttaagcttt tgcggattga tctccaaata ctcatcgacg attgaagttt gtccatctaa
    26881 acaaaagttt actttagggt tcctttccaa ttcatctaga acttcagtaa agacctgatc
    26941 agacaatacg atggcatctt catttgtaaa ataccattct ctgtcccagt gcgtgtgttg
    27001 aattatatgt gcatttacca tatcagattc tcctactttc cttcttctac tgaatttact
    27061 tgttgttctt ttttatactt gatggatttt agtgcattga ccattaacgc tgtaaccact
    27121 cccccaataa caattgatac taaatataaa ggtatattcg taacaaaagg aattgccata
    27181 aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc catttagacc acatgctact
    27241 gcagtgccca ccacgatgct tggtattact cgtaaaggat ctttcacggc aaacgggatc
    27301 gtcccttcag taatccccac aaggcccata atcatagctg tctttgcatt atctcgttcc
    27361 gattcatcat aaagtttttt gtttaaaatt gttgctaaaa acattccaaa tggcggaata
    27421 gcaattccta ataacatggc tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc
    27481 ccaaaagcat atgcgatttt attgataggt cctcccatat caaacgccat cattcctcct
    27541 aaaatagccg acatcaaagc agcatttgct cctgacatgc cttctagcca attagtcagt
    27601 cctgttgtca ttgcttgaat aggcgtagct agtacatacc ataatatgat tccaacaact
    27661 aatgtagtaa caaaaggaat cagcataatt gattttagac taataacttg catcggtact
    27721 tttacttttt tcaatgcatt agctaaatag ccacaagcta atccagcaac cattcctcca
    27781 ataaatccca taccaagatt ttgacacatc atcccagtga taaaagctgg tgctagaccg
    27841 ggacggtcag caattgaata tgcgatatag gctgaaatca tcggtacgat aaactgtaat
    27901 cccgtctgac caatcattcg caggactccc cagaatgtat ccgtctggtc ccaaacacct
    27961 gtgccaccaa aaatgactgc aatagaagtt agtaaccctg ctgctacgac aagcgggatc
    28021 atataggaaa ccccactttg gaaatgattt agaatttctc ttccttgttt cttgaattcc
    28081 attagtaact cccccacgat atatttttta tttgttaatt aatgcaattg cttcatcaat
    28141 cgtctcttca ccatctttta ctacctctga ggtccctact gttaaaatcg gtttaccgtc
    28201 aaagcgacct cgttctgaaa catttgtatc tactgcaaat atgacaacat ctgcctcgga
    28261 aacatccgaa attgaaagtt tgttttctat ccccatagcc ccctgtgttt ctactttaat
    28321 atctaaccct ttcttcttcg caaacttttc taaattagct tttgccatat aagtatgggc
    28381 aatccctgcg atacatgctg ttactgctac tatttttgtc atatgaaatt cctcctttta
    28441 aaatgataga gcttttacca attctttttt atttctcgaa tcttttattc ttttaacaaa
    28501 ctcgtcatcc atcaatcttc cagataattc tgctaaaata cgtaaatgat tagtaccttt
    28561 atcagtgtct tttattgcta acaaaaagac gagatcaact ggttgatcat ctaatgcgcc
    28621 ccattcaaca gaccgcttta acttggctac agccactgtt gattcaatta ctgctaggct
    28681 tttgccatga ggaatggcga tatttttccc aattcctgtg gtcatatggt tttctctgtc
    28741 aaatacgtct ttttgaaatt gtttgtggtc agacacaaac ccattagtct ctaacaattt
    28801 cgataatttt tcgactacac tttttttatc agatgcattt aaatctatca ctatattatt
    28861 tatattgatt ttttcactga tcatttataa gccttctttc aatatataaa ctaatttttc
    28921 aaacgacgaa ctcgttttta aatttgctaa taactcatga ttcgaagcga tttcattgaa
    28981 tgtttcataa attttattca attcaggttt tactttttca ttaagtgcaa aaaagaaaac
    29041 agaatgaaca agtgtcccat cccaatcaat tccttctgga ttaacgtaga cacagattac
    29101 tggctttttt ataaaatcta cttcgatatg cggcatagca aaatcattca tcgctgttga
    29161 agatagtttt tctcttttga ttgctccctc tagtatccct tctttcgcgt aattttcttt
    29221 taccaatttt ttggtaatta tatttaaaac gttcacttga ttatccttta tttggtgggt
    29281 aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca tcttctgttt gttcatttaa
    29341 ctgtagatac tgttttactt ttctgacatc tgcccctaac atcaaaggac taacaacaat
    29401 aacaggaaaa tttgtatttt caatcggaat cgtgcttata acaagttcat cagtgacttt
    29461 tctatcttgt agttcattga tgcttagtac atcttctatc tcaaccatat ctgcaaaacc
    29521 gttatttatt ctttgctcta ataattttga tgttccatat ccgctactac aaactaatat
    29581 cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat tgaatatgga gtgttacgta
    29641 agcgatttca tcttcattaa aatgtataca gaatctctct tcgatttcct ctaataaatc
    29701 tacacttatc ataaatgacc ttttaaaaga atatttgatt ttgtccgtat aagggttata
    29761 gatatttact cccaatttga gtcttttaac tgctgcattg agatgtaata ctagattatt
    29821 gatcaattct tcatcaggat aaaattcatt taatttaatt cttactaact cttgtagctc
    29881 aacacccata tctaaatagg aaagcttcgt ttgatctaag tcgtgattca tagcattttt
    29941 ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca tctattggca caataattga
    30001 aaatgctttt tcaatcattt tgattaaaat aagtgtattc tttgagattt cttcttcaga
    30061 attattacta cgtgataaga aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg
    30121 gatagctaaa gtttgaaatt cataatctgt aaatttcaat tgtgtcttat tcatgtatcg
    30181 attaagcaat tcggctactt ttttcaatat gtccttattt aagaaattat tcgcttcacc
    30241 aatgaaattg atattcaagt ctaagagatc tgttttactg ttagatgctg taaagccacc
    30301 ccaataatag ttcacaactt ctgacatcaa ccttctttta cttttttcat cagctttgat
    30361 gcgcacgcca tttcgatctg aaactaagga catcccatgc ttgtttagtc tttttctaac
    30421 atctttcatt gtgctttcta ccgtcgacct gctcacgtat aattcgtcag caagattttg
    30481 aatctttaga gaatcattag tattcaataa tttagcaaac acataagtta gacggtcctc
    30541 cggagagtct gcgatattga ttttatgact ggataataaa tttcgaagtg tttctttttt
    30601 tccaataagc gaaacgcctt ttttaggttg aaccaacatt tgtacatctg gcattttttg
    30661 gattatttct ttcaaaatta aaatatcttt gctaattgtt ttcgtagata catataattg
    30721 ttctgataat tctttgtaag ttgtgtgctc attttcaatc aaaaagtata gtattttcat
    30781 ttgccgttcg ctaaattcga tgcccattat cttcacctcc tgaatacagc ttaagtgtac
    30841 aattattatc tctattttca tacagaagaa agttcttgat taatgtagaa gtttctaata
    30901 ttcttgatta tttcaaatca ataaaaaagc ctgcaatatt tatatcacag gcttttttct
    30961 ctattaaata tatataagat aatacatttt tctactagtt ctcactaatc ttgtataatt
    31021 aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct
    31081 ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg
    31141 tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt
    31201 gcgttcctgg tttgatagga ttggccactg ttaatcgaat gaatggatcc tcttttgaaa
    31261 atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca
    31321 ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg
    31381 ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct
    31441 ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa
    31501 gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta
    31561 aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa
    31621 tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt
    31681 cttccatttc ttgaaatgct tgtccttttt gttcattttc atccaaccat gtattcagtg
    31741 catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt
    31801 cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga
    31861 agaaaataaa acttcccata aagataacaa cgagaagcaa cagccattta atcaaatcta
    31921 aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag cttccctcga
    31981 agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact
    32041 gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc
    32101 agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac
    32161 tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca
    32221 gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt
    32281 ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt
    32341 tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct
    32401 tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt
    32461 aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga
    32521 acctcctcca gtaaggaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc
    32581 aggaccacct acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc
    32641 acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc
    32701 gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct
    32761 ttgatttttg cgaaaacacc gggaatgaag gccgttccca tgatataaag aaaagaaaga
    32821 agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc
    32881 cctaatgtca gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg
    32941 attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caagcatggc tagtcccgtg
    33001 atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa
    33061 gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc
    33121 ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttct
    33181 accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg
    33241 ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc
    33301 agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc agcagttaat
    33361 agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc
    33421 aactaggtga ataccatcca caggaacttt tgcttctacc aatgcttgat tgatggctgc
    33481 tacttggtca attccttcaa aaagctggat aacacggcta gattgtacaa aataatccct
    33541 ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat
    33601 aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc
    33661 atgcaaaata ccatagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag
    33721 caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat
    33781 ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat
    33841 cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt
    33901 tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac
    33961 accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc
    34021 aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa
    34081 taattcgatc gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga
    34141 cttacccgct ccattttccc caatcaatcc atagatcatt ccacgtttga tttcgattga
    34201 tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac
    34261 cgtttcattt ccaaaatcca tacttatcct tctttcttat tgatgagtga agtatagttg
    34321 acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatg
    34381 tcaaaaaaaa caatcggaca atacgtgtct ttcctattat caggcagttt gttatacgct
    34441 tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac
    34501 tgaaaatttt tgcttctttc agatcaagct tgagtaattt taccagaccg gttataagga
    34561 taactatagc aagactccct cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg
    34621 tcttccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa
    34681 tcgttgttat cgttgacata agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc
    34741 agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat
    34801 acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa aagacttttc
    34861 acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    34921 tatttatcaa acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca
    34981 cttctgtcac aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc
    35041 ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta
    35101 aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt
    35161 aactgcgctc agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata
    35221 atcgcaatta acgttttgag aactgtgaag ctttacgggc tttcttaaga cctggttttt
    35281 tcttcgtatc attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat
    35341 attttattat atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa
    35401 ggcagtatat ttcgtatttt agattaaatt tttaggctca ttc
//
LOCUS       DAZKPO010000021        35017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000021 DAZKPO010000000
VERSION     DAZKPO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.021"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1917
                     /gene="efbA"
                     /locus_tag="ACG8NZ_001011"
     CDS             211..1917
                     /gene="efbA"
                     /locus_tag="ACG8NZ_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0538705.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="ACG8NZ_001012"
     CDS             complement(2075..2977)
                     /locus_tag="ACG8NZ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0538706.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3666)
                     /locus_tag="ACG8NZ_001013"
     CDS             complement(2974..3666)
                     /locus_tag="ACG8NZ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0538707.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="ACG8NZ_001014"
     CDS             complement(3709..4350)
                     /locus_tag="ACG8NZ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0538708.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4388..4702)
                     /locus_tag="ACG8NZ_001015"
     CDS             complement(4388..4702)
                     /locus_tag="ACG8NZ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538709.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG8NZ_001016"
     CDS             complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG8NZ_001016"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0538710.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG8NZ_001017"
     CDS             complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG8NZ_001017"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0538711.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6039..6971)
                     /locus_tag="ACG8NZ_001018"
     CDS             complement(6039..6971)
                     /locus_tag="ACG8NZ_001018"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0538712.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6968..7744)
                     /locus_tag="ACG8NZ_001019"
     CDS             complement(6968..7744)
                     /locus_tag="ACG8NZ_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0538713.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG8NZ_001020"
     CDS             complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG8NZ_001020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0538714.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10988..12064)
                     /locus_tag="ACG8NZ_001021"
     CDS             complement(10988..12064)
                     /locus_tag="ACG8NZ_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0538715.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12069..13349)
                     /locus_tag="ACG8NZ_001022"
     CDS             complement(12069..13349)
                     /locus_tag="ACG8NZ_001022"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0538716.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13368..14294)
                     /locus_tag="ACG8NZ_001023"
     CDS             complement(13368..14294)
                     /locus_tag="ACG8NZ_001023"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0538717.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14307..15590)
                     /locus_tag="ACG8NZ_001024"
     CDS             complement(14307..15590)
                     /locus_tag="ACG8NZ_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0538718.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG8NZ_001025"
     CDS             complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG8NZ_001025"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0538719.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16378..17289)
                     /locus_tag="ACG8NZ_001026"
     CDS             complement(16378..17289)
                     /locus_tag="ACG8NZ_001026"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0538720.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG8NZ_001027"
     CDS             complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG8NZ_001027"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0538721.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17755..18453)
                     /locus_tag="ACG8NZ_001028"
     CDS             complement(17755..18453)
                     /locus_tag="ACG8NZ_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0538722.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18440..18826)
                     /locus_tag="ACG8NZ_001029"
     CDS             complement(18440..18826)
                     /locus_tag="ACG8NZ_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538723.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18973..20643)
                     /locus_tag="ACG8NZ_001030"
     CDS             complement(18973..20643)
                     /locus_tag="ACG8NZ_001030"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0538724.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20826..21059
                     /locus_tag="ACG8NZ_001031"
     CDS             20826..21059
                     /locus_tag="ACG8NZ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0538725.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21158..21589)
                     /locus_tag="ACG8NZ_001032"
     CDS             complement(21158..21589)
                     /locus_tag="ACG8NZ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0538726.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21668..22081
                     /locus_tag="ACG8NZ_001033"
     CDS             21668..22081
                     /locus_tag="ACG8NZ_001033"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0538727.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22119..23267)
                     /locus_tag="ACG8NZ_001034"
     CDS             complement(22119..23267)
                     /locus_tag="ACG8NZ_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0538728.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23389..23985)
                     /locus_tag="ACG8NZ_001035"
     CDS             complement(23389..23985)
                     /locus_tag="ACG8NZ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0538729.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24185..26827)
                     /gene="alaS"
                     /locus_tag="ACG8NZ_001036"
     CDS             complement(24185..26827)
                     /gene="alaS"
                     /locus_tag="ACG8NZ_001036"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0538730.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27215..27646
                     /locus_tag="ACG8NZ_001037"
     CDS             27215..27646
                     /locus_tag="ACG8NZ_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0538731.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27691..29040)
                     /locus_tag="ACG8NZ_001038"
     CDS             complement(27691..29040)
                     /locus_tag="ACG8NZ_001038"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0538732.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29110..30273)
                     /locus_tag="ACG8NZ_001039"
     CDS             complement(29110..30273)
                     /locus_tag="ACG8NZ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0538733.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30402..30725
                     /locus_tag="ACG8NZ_001040"
     CDS             30402..30725
                     /locus_tag="ACG8NZ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538734.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30908..32257)
                     /locus_tag="ACG8NZ_001041"
     CDS             complement(30908..32257)
                     /locus_tag="ACG8NZ_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0538735.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32326..33279)
                     /locus_tag="ACG8NZ_001042"
     CDS             complement(32326..33279)
                     /locus_tag="ACG8NZ_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0538736.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33381..34571)
                     /locus_tag="ACG8NZ_001043"
     CDS             complement(33381..34571)
                     /locus_tag="ACG8NZ_001043"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0538737.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34771..>35017)
                     /locus_tag="ACG8NZ_001044"
     CDS             complement(34771..>35017)
                     /locus_tag="ACG8NZ_001044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538738.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9968 a         7650 c         6130 g        11269 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc cttaccaaaa aatagagaaa atctatatat aatagaaaaa agagaagaga
     4501 ttattctctt ctttctgcta accagtctta gaaaaatgaa ttttaaaaaa atcaaaaact
     4561 gatcacgata gaagtttctt ttcatacctg atgaaaagat gcttctcgca atcagttttt
     4621 ggatttttgc tattccaatt gatgattctg gagacacaaa tcggatttct ctatcttcat
     4681 caagcgcgct taaatctgcc aattttcagg atcttttttc cattctttta gcagagttaa
     4741 ttcttgctca tcgatataac gatcttctaa agctgcttca atcaatacag aataattggt
     4801 caacgtcata agagggattt cggctttttc gaaatttgct tttccttttg gcaactcata
     4861 cgtaaagata gccgcaacac caaggatatc cgcaccttct ctttttgctg cttcagctgc
     4921 ttccaagaca cttccgcctg tagaaatcaa atcctcgatc acaaccatct tctgaccttc
     4981 aaatatacgt ccttcgattt ggttaccttt cccatgatct ttcgccttac ttcgaatata
     5041 gaccattggc aaatccagta tttccgctac ccatgcggca tgagggattc ctgctgttgc
     5101 agttccagca atcacttgta cttcagggta agcttctttg attttgcttg ctaatccttt
     5161 tgcgatctct tttcggactt ttggatagct catcgtgatt cggttatcac agtaaattgg
     5221 gcttttgatt ccacttgccc atgtaaaagg attacttgga tttaaaaata ctgcttcaat
     5281 ttctaataag tcttttgcga tactttgttc aacattcatt ttaggttagc tccattctgt
     5341 ttgaatcgtt tgatatgctt tataaggatt agttgactga gtgattggtc gacctataac
     5401 gataaaagtt gagccgatct tttttgcctg agaaggtgtg actactcttg tctgatctcc
     5461 tgcgactgtt ccttctggtc ttatacccgg agttaagcaa acaaattcgt ctcctgccat
     5521 ttctttgatc gcttctactt cccaagcaga agaaaccaca ccatctaatc ctgctttttt
     5581 ggcacagcta gcataatgga tcacactttt ttctaaagaa acttcgatta gttgatcggc
     5641 atgcatttct tcttcacttg tagaagtaag ctgagtgata gctagaagta gcggacgttt
     5701 tccaccttct ggagtcccct cttctaatcc tcgcattgcc gcctccatca tacgaatacc
     5761 gccagcggca tgaacacagg tgagatcaac accaagcttt gccaatcccc gcattgcttt
     5821 ttcgaccgta ttcggaatat cgtgaagttt cagatctaaa aatacattat gaccagcctc
     5881 ttttagataa cgtacgattt caggtccttc ttggtagaac aattccattc cgattttcac
     5941 aaacaatttt tcttctttcg ggaaaaatcg aagaaagtct tctacctctt gcctagttga
     6001 aaaatctaat gcaataattg gtcgttggtc tagttgattc atcgttcacg ttcctctctc
     6061 acttctttga ttaatctttc cagcgattcg atgcctagtt cgctcattcg cacagggagc
     6121 tctttaatta gtttaggaca gatatacgga tcggtgaaat tcgcggtccc tatagctaca
     6181 gcacttgctc cagccataaa catctcaaga acatcatcca ctgtctgcac gccgcccatc
     6241 ccaatgattg gaaggctgga tatacttgct acttggtgaa tcaaacgaat agcgacaggc
     6301 ttgattgctg gtccggagag tccacctgtt tgattggcta aaatcggttt tcttgtctta
     6361 aggtcaatcc gcataccaag aagagtattg atcatcgtaa acccatctgc ccctccagct
     6421 tcgattgcct gagcgattgg aacgatgtct gttacatttg gggacaattt gacatagaca
     6481 ggaacagaag aaactttctt tactgcttgt gttaattgaa aagcaacatc agaatctgtt
     6541 ccaaaagcaa ttccgccatg tttgacattt gggcaagaaa tgttcagctc gattgctttc
     6601 acatttggtg cttcgccaat tttgctacaa acttctacat agtcttcttc acaagcacca
     6661 gcaacatttg cgatgatggg taactcattg tactcctcta attctggaag aagtttatgc
     6721 ataacaacgt ctagaccagg attttgcaaa ccaattgcat tcaacatgcc agatggtgtt
     6781 tccgccactc ttggcgtggg attgccaaag cgggcatttg gagtcgttgc cttgaccatg
     6841 atcgatccaa gctgattcaa atcatagtat tttccgtatt ctttgccaaa accgaaacag
     6901 ccgcttgccg gcatgatagg attttttaat tctaggccag gaagtttgac cgctaaagga
     6961 ttctcattca tataattacc tctcctattt taaaaatcgg tccttcgtca caaactttca
     7021 cactttttgt tcctgtttca tcttccggcg tatgacaaac gcaagcatag caagccccta
     7081 tcccacatgc catgcgggat tctaatgata actggacatt ttgatgcgaa gaaaacagct
     7141 gttccactgt cttcaataac ccgtttgaac cacatgcaaa aacagctgta ggttccgtct
     7201 tcgttcctaa caataaatta ccgacatttc cgtgcatacc gtatgttcca tcgtcagtag
     7261 cgattcttgt ttctcctaga cgattaaatt ctttttcata ataaacgact tcatgtgtgg
     7321 caaaacctaa aaaatgcgtt ggcttaattc ctttttcgac taactgtttt gataactcat
     7381 atagaggagg gacaccgatt cctccaccga ttataaatac ttcgtctcct gtatgaagat
     7441 tggacaaatc aaaaccattg cctaaaggac ccatgacatc taaatggtct ccggcagaaa
     7501 gagtagagaa gactctagtc ccctcacctt ccacccgata aatgatacgg caagtatttg
     7561 tttcgtggtc gatatgattc aagctaatcg gtcggcgtaa tagcaagtct gcacgcggca
     7621 cgagaatatg caagaactgt cctggttgct gcatttcttt tactagatca cctttcaatg
     7681 tcatttcaaa aatctttggt gccagctgtt tttgctgaac gatttccatc atttcttgtt
     7741 tcatctgtcc cctcctgcaa cggtttttcg tctttttgtc ttaacatgtg ctcgccgtta
     7801 aatagggtta gttgaaaaag cacgggattc catcactcga acgatcgcat ctgctgtgtc
     7861 taaagaagta aacaatggaa taccatgttc aactgcttct cttcggatga tgaaaccgtc
     7921 tttagaagca tttccccgat ctttatcaat cgtattgaca accacttgtg ttttaccatc
     7981 tcttattaag tcgactacat ttttttctgc tgcttcctca gaaattttcg ctactgtttc
     8041 aacgaccaga ccattttttt cgaaataagc agctgtatgt ttcgttccta tcagactata
     8101 gccaatttct gtaaaacgtt tagctaattc tagtgcttct tcttttgttt cgtccgcaat
     8161 cgtaaataat acggctccat attcaggcaa gtgtaatcca gaagcttcga atgccttgta
     8221 caaagctttg tctaattgtc tgtcagatcc cattacttct ccagtcgatt tcatttcagg
     8281 ccctaagtaa gtatcaactt tctgcaattt cgtaaaagaa aagacaggtg ctttaacatg
     8341 aacttctttt gtttctggat aaagtccgtt ttcataacca agtgtcttta gtttctctcc
     8401 caaaatagct ttcgtcgcaa tttgtgccat tggaattcct gtcaccttac ttaagaatgg
     8461 aacagtccga cttgctcgag gattgacttc gatcacataa acctcatttt catggatgac
     8521 aaactggata ttcatcatcc cgacacaatt caaacctaat gctaagcgga tcgtgtaatc
     8581 ttcgatcgtt ttcttgatcg attcagataa tgtttgaggt ggatacacag ccattgaatc
     8641 ccctgaatgg acaccagcac gctcaatatg ctccatgatc cctgggatga gtactgtttc
     8701 accgtcacaa atcgcatcca cttcgcattc accaccaagc aagtaacggt ccactagtac
     8761 tggatgttct ggagatgctt tcacagcatg ttccatgtaa tcttctaaat ctcgctggtt
     8821 ttcaacgatt tccatcgcac gtccgcctaa tacatagctt ggacgaacaa gaactggata
     8881 accaattctc gaagcgataa ccacggcttc ttctttactt gtagcagtat cccctaaagg
     8941 ttgaggaata tccaactctt tcagcgcttg ctcgaataaa tcacgatttt ccgcacgatc
     9001 caagtcttcg atcgttgtcc ctaagatttt tacaccagca gcagtcaaag gttcggctaa
     9061 gttgattgct gtctgaccac caaattgaac gattactcct atcggctgtt ctagctcgat
     9121 cacattcatc acatcttcga aggtcaatgg ctcaaaatat aatttatccg aaatagaaaa
     9181 gtcagtcgaa actgtttcag gattgctgtt catgatgatt gcttcgtatc ctgcctgctg
     9241 gattgctttg acagaatgca cagtcgcata gtcaaattca accccttgtc cgatacggat
     9301 aggaccagat cccagtacta gaattgaggg cttttctgat cgtgaacttt cattttcata
     9361 ttcataggaa ctataaaaat aaggtgtttt tgattcgaat tccgctgcac aagtgtcaac
     9421 catcttatat acaggaatca aatcattttc ttttctcatc tggcgaacgg cttcttcgtt
     9481 tatttcccac agctccgcca cttttttgtc tgtaaaaccg ttttgtttcg ctgttttcaa
     9541 tatcttgtca tccaacggct gctgtttcaa ctgctgttcg atttctacga tatgcaacag
     9601 tttatctaaa aagaataaat cgatttttgt caaatccgct aattcttcta tggtgtaccc
     9661 tcttctaata gcttcagcta cataaaacag gcgatcatct tgtgctcgaa ccactttttg
     9721 gattaggtca tcgtccgtga tgtctgccaa ctcaggaagt tcgatatggt gtgtaccgat
     9781 ttctaaagaa cgcacagctt tcaataaaga ctcttcgata ttccgaccga tagccatcac
     9841 ttcaccagtt gctttcatct gcgtgcctaa aagccgttct ccgttttcaa acttatcaaa
     9901 cggccagcga ggaattttag ctacaacata atctaatgca ggttcaaatt ctgcaaaagt
     9961 cgttcctgta acaggatttt tcatttcgtc taatgttaat ccaagcgcta ttttagcagc
    10021 tagcttagcg atcggatagc cagtcgcctt acttgctaac gcagatgaac gagaaacacg
    10081 cggattgact tcaatcacat aataattaaa actatgtgga tcaagagcga gttggacatt
    10141 acagcctccc tcgatcttaa gtgctcgaat aatcgaaaga gaagcatcac gaagcatttg
    10201 atattcatgg tcagacaatg tttgactagg ggcaaatacg atagaatcac ctgtatggat
    10261 acccaccggg tcaaaatttt ccatattgca gacaacgatt gcattatcag ccgaatctct
    10321 catcacttca tattcaattt ctttaaaacc tgcaatactt cgttcaatca aacattgggt
    10381 agcgggggaa agacttaatc cattttctga tacgatacgt aattcttctt cattttcaca
    10441 catacctcca ccagttccgc ctaaagtaaa ggctggacga acgatgacag gatagccgat
    10501 tttatttgca aaatctacag cttcttctac tgtattgacg atttcagact cggggattgg
    10561 ctgattcaat tctttcatca actgtttgaa tagatcacga tcttctgctt gatcgatcgc
    10621 agaaagcttt gttcctagaa gttctatgtt caattcttct aaaatacctg atgctgccag
    10681 ctccattgcc atattgaggc ctgtctgtcc gcctaaagtt ggaaggagag catctggacg
    10741 ctcttttcga agaatccttg atacaaattc aagtgtgatc ggttcgatat atacttgatc
    10801 tgccacttct ttatccgtca tgatcgtcgc aggattagaa tttactaaaa ccacttcata
    10861 tccttcttct tttaaggcaa ggcaggcttg tgttcctgcg tagtcaaatt ctgcggcttg
    10921 accaatgatg atcggaccag atccaatcac cataattttc ttgatatctg tacgtttcgg
    10981 catctgttta ctgctccttt cctgcgtcca tcatttccat aaattcatca aataaatgta
    11041 gtgcatcatg cggaccaggg gcagcatctg gatgaaactg cacactgaaa gcgggataat
    11101 ctctgtgttt gactccttct actgttccgt catttacttc tacatgagtt acgatgagtt
    11161 tttctggatc aatcgtggct tcatcgactg catagccatg attttgagaa gtaaaatcaa
    11221 ttcttccggt cgctacttct cgtaccgggt gatttaatcc tcgatgtccg aatttcattt
    11281 tataggtatc tgcaccgttg gctaaagcaa ataactggtg accgagacaa ataccaaaaa
    11341 tcggtacttt tccttggatt tttttgatca tttcgattgc ttctggcaca tcttttggat
    11401 caccaggtcc attcgtcagc atgactccgt ctggtgatag cgctaaaatc gtctcggcat
    11461 ctgtgttata gggaagtact gtgaggttac agtttctttt actcaattct cgtaaaatac
    11521 tatgtttcaa gccgaagtca atgacaatga ctttccgacc tgttcctgga cttggataag
    11581 gtttgttagt tgagacttgc tgcacttgat tggttgctag aacggttgct ttcagctgat
    11641 caaatgcatg ttcgaaggaa tctccagcat caactatact agctttcatc gttccgactt
    11701 cacggatttt gcgagtaagg gcgcgcgtat cgatacctga tattccagga attccttttt
    11761 gtttcaaaaa tgaatctagc gtcatttggt ttcgccagtt tgaagctaca cgtgcatgtt
    11821 cttttactac tactcctttg catgttggag aaatcgattc ataatcatct ctatttaccc
    11881 cataattacc caccatagga taggtaaatg taatgatttg accgttaaaa ctttgatcag
    11941 tgatagtttc ttgataacca gtcatacttg ttgtaaaaac gatttctccg aagacactag
    12001 catctgcacc aaaagccttt ccttcgaaaa ctgttccatc ttctaatata agcaaacgtt
    12061 ccatactttt attctccctt ctgccacaca agttttccgt tgacaaaagt catttgtgtt
    12121 tcgcctttta ctttccagcc gataaaaggt gtattctccc cttttgataa aaagtcttct
    12181 ttatcgatcg tgcaagtttg tgtaatatca aaaactgcta catcagcggg ggcaccaacc
    12241 gttagcgttc ctgcattcaa accaaaaatc tcagccggtt taactgccaa ccaattgatc
    12301 acttgttcca acgtgaatcg accagtttct acaaaatgag tatagatcag ttgaaacgct
    12361 gtttcgcttc ctacgatacc aaatggtgat ttcatgaaac tttggctttt ttcttctaat
    12421 ccatgggggg catggtctgt agcgatgcaa tcgatcgtac cgtcaagcaa gccttcgatc
    12481 aaagcttctc tgtcctcccg tcctcttagc ggcgggttca ttttccaaaa gccgaaatct
    12541 tcgggaatat cttcgtcgat caaaataaga tgatggggtg aaacttctgc tgttacgtgg
    12601 attcctgctt tttttgcgtc tctgattaca cggacacttt ctttagtaga aacatggcaa
    12661 acatggtaat gaacaccagt tgcttcagct agcaacagat ctctagcaat ttgagaagac
    12721 tcggttacgc ttaagatccc gggaagacca agttcttctg ccttcttgcc agcgtgcata
    12781 acgccgccaa ataataaaga ttcatcttct gtatgggcta ctagggcttt attgtttttt
    12841 gcagcttctt tcatagccag atacatcgtc ccagctgttt ggacacctac accgtcattt
    12901 gtgaaagcaa aagcaccttc ttcaatcagt gcttcttgat caactaattt ctcacttcgc
    12961 agattttctg tgattggcgc atactgcaat accttcacaa ctgcatcttt tctaatgatg
    13021 tcatatactt gtctcagctt ttctgccgta tcaggtacag gatttaaatt cggcatcgcg
    13081 catacagtag tgaaaccgcc acgagcagct gagcgagtcc cagcttcgat cgtctccttg
    13141 tatgtaaatc ctggctctct caaatgaaca tggacgtcca ctaaaccagg tgtgatcaat
    13201 tgtccctttg catcaaacac ttcatcaaat tcggcttctg agaatcctgt accgattgct
    13261 ttgattcgcc catcttctat ccaaatttct gctggtgtcg tcatattttt tcgtgtattg
    13321 atctgaccgt ttttaatgag tgttttcatt ttttcatcct cctataatta cgcttttcca
    13381 tttagcactg cttctaagat tgccattctg acgaatactc cattgctcat ttgctgaacg
    13441 atccgcgact gtaatccttc cactaatgaa tcagctaatt ctacatcacg gttaactggt
    13501 gctgggtgca taatgattgc atgtttttgc aacttcttcg cacgctcaac tgttaaccca
    13561 tgttcctgat ggtagccttc ttttgagaaa ctttcttttc catcatggcg ttcgtgttgc
    13621 acccgaagca tcatcatgac atcaacttga tctagtagtt catccaatgg catataatgt
    13681 ccgtacactt cgaattcatc gtcgtaccat tctctaggac ctgagaagaa tacttgtgcg
    13741 cctagacgtt ttaacatctg catattggat ttagctacgc gagagtgagt gatatcacct
    13801 acgatggcaa ctttcagacc ttcaaaatga ccaaattctt cataaatggt catcaaatca
    13861 agcaagcatt gggttggatg ctgtccgctt ccgtctcctc cattgataat cgaacactgg
    13921 attgttttac tttgtatcag ttcgtcataa tagttctcat caccatgacg gataaccgct
    13981 acgtctacgc ctaatgctga catcgtcagt actgtatcgt atagtgtttc ccctttttgg
    14041 acagagcttg tgcttgcttc aaattcaatc acttctagcc ctagtttttt ctccgctaca
    14101 tcgaagcttt tatgtgtgcg agtactattt tcgaaaaaga ggtttgtagc aaagtactgg
    14161 cgcttttgcg gagtccaatt agcaccttgc ttgaattcct gccctctgcg gatcaagccc
    14221 ataacttctt gatcagtaag tgcctcaacg gtcaataaat gctttaagct gatacgttct
    14281 gatttgatga tcataatgtt tcctccttag ttttcttcgc ttcgtgaagt ttctggtaag
    14341 ataagattta aaatgatgcc aagtacagtt gctaatgcca ttgccgacaa tgtaaatgtg
    14401 cctacttcaa agacgagtcc gccaattcct actactaaga taactgaagc aatcaacaga
    14461 tttttctttt tatcaaaatc gattttgttc tcgattaaaa tcttcattcc acttgcggca
    14521 attactccaa ataagataaa actgattcct gagatgactg gtccagggat acttaaaatc
    14581 aaagcgctta attttcctac gaatcctaaa atgactgcaa atactgcagc tccaccgatc
    14641 acaaacacgc tatggacacg tgtgatagcc agaacaccaa tgttttcacc gtaacttgtt
    14701 acaggaggac ctccgacaaa ggctgccacg atttgtgcta atccatcacc tgtcaacgtc
    14761 ttatgaagac ccggttcttc aaagaaatta cgtttagtta gtttattcaa taccataaga
    14821 tggccgatat gctctgtcat cgtcacaaaa gcgatgggtg ccattgttgt gattgctcca
    14881 agatatagct ttggttcata ttgtacaaag ggaatctcaa agttaggaac agcaaaccag
    14941 gcagcgtcta cgattggctt agtatccacg attccaaaca gcaaggctac gatgtatccg
    15001 ctaataatac ctagtaaaat cggaatcaat ccgagaaaac ctcttagcca catgttgaag
    15061 aaaattgtta atcctaatgt gatcagcgct acagcaatat acttgaaatc atacgtgccc
    15121 tgattataca tagcatttgt tgctgcattt gccgcaagtc ccaacccgat aaccatgatg
    15181 actggaccga cgacgattgg aggcaagatt ttatccagcc atgctgaacc gatctttttg
    15241 atgatcagcg caacaatcaa atagactaat ccagttgtga ctgctccttg tgcaatcgct
    15301 ggataaccat ctgttttcat caaaagctgc atcgctgcaa tgaaagcgaa gctgcttcct
    15361 aagtacgcag gtatctttcc tttagtagta aataagtaca ccaatgtacc tagtcctgaa
    15421 ctgactagcg caatacctgg atcgatacct actaagatgg ggactaacac ggttgctccg
    15481 aacatcgtga acaagtgttg taaacttaat cctacccaat gtcctggggt tgggcgatcg
    15541 tggatatcta acaccacgtc atcattatga aatccttttt tctccgacat gtttctctct
    15601 cctctaatct tcctctttca aaatcatgat gcgatcttgt ccatcaagct cttgcatctc
    15661 tactaaaatc tcttccgccc gagatgttgg aatgttcttc ccaacataat ctgcacggat
    15721 tggcaattct cgatgcccac gatcgaccag gactgctaat gaaatttttc ttggtctgcc
    15781 aaaatccatt actgcgtcca ttgctgctcg aatcgttcgt cctgtataca aaacatcatc
    15841 aatcaaaata acttcttttc cttctaagga aactgggatg tcagaagaat gaagttccgg
    15901 ttcttctgga ttttcttttt tgtcatctcg gtataaagtg atatccagtt ctcctacagg
    15961 aatatcgata tcttctagct gcttcaggcg ttcggcaatt cttgatgcaa tgtagattcc
    16021 tcgtgttttg atacctacca atacgatatc ttggatacta tggtttctct cgataatctc
    16081 ataagtaatt cgagtcaatg ctcgcttcat ggttacctga tctactactt cttttgcttg
    16141 cattttcatt ccccctactt ttttgcataa aaaaactcct gccctaagag gacaggagga
    16201 atgtttagac tgagcgtata ctgatacact gatagctata ctatgtcaat gagcaatcta
    16261 cttatggcgc accatacgta tccttgctcc cacccttctt agcctcacgg gactaaactt
    16321 aaaggaattc tattaagttt tatgctttcg ttgttaatta ctataccgtt atctaaatta
    16381 aaatgcaatg ttttttctta atttttctaa agttttttca aaaacttctg gaagaggtgc
    16441 ttcaaagatc atcttttcgt ttgtagtcgg gtgcgtaaaa cctaacattt gcgcatggag
    16501 aaactgacca ttgcctttca aagttttctt cggtccgtat actggatctc cagctaacgg
    16561 ataaccgata tacttcatat gcacacggat ctggtgtgtt cttccggttt ctagttttaa
    16621 ttcaaccaga gtaaatcctt cgaaacgttc caatacagta aaatgtgtga cagccgactt
    16681 cccatcttct cttatcgcct gcattttccg gttttccttc gaacggccga ttggcgcatt
    16741 gatggttcct ttttcgtgag gaatctctcc atggactaaa gcaacatatt tgcgcaaaga
    16801 ggtcttatct tttaattgct gagctaatga ttcgtgtgcc tggtcatttt tggcaaccat
    16861 tagcagtccg gaagtatctt tgtcgattcg gtgaacaata cctggacgaa taacgccgtt
    16921 gatcgtagaa agatccttga tatggtacat cagcgcattg accattgtgc catgcgtatg
    16981 acctgctgaa ggatgaacga ccattccttg aggtttgttt atcaccgcta catcttcatc
    17041 ttcgtaaacg atcgtcaacg ggatattttc tgggaccaca tctaatgttt ctggttcggg
    17101 gatcaagaca gtaatctcgt ctccttgttt tactttgtaa tttgctttga ctggctgtcc
    17161 attgacagaa accgctccat ttttcagcca cagctgaatt tgcgaacgac tgtaatcggt
    17221 gaataaatta gtcaatactt tatcgatcct accagattca tggtgaatcg tcgttttaag
    17281 ttctgtcatt tttttctttg atccttttca tctaaaataa tataaattaa catacaaatc
    17341 actccacata caagtgtcac atctgctacg ttaaaaatcg gaaagctcat aaaatctgtc
    17401 tggaacatat ctactacata gccaagacga atacggtcga taaaattgcc aatcgcacca
    17461 gctagaatca aactcaatcc aatcatgaac cagcgattac ctttttcacg atttttaatc
    17521 atcaatgtga gaatcacaac gactgcaatg agtgtcacta tagtaaagaa ccacatcttc
    17581 ccttctagca tactccatgc ggctccagtg tttcgaatat gattcagtga caaaatacct
    17641 ggtataagat ttttcgtctc tccaagcgct agattatcag tgatccacca tttgaccaac
    17701 tgatccactc ctactagcaa agcgctgaat atccaataaa taattaacaa aaaatcaatc
    17761 cttttctatt tgtttataaa tatcacgagg tcgtacgatc ccgagcagat taccatcggg
    17821 attcccatct ttagtgataa gtacggcttc caaagtccct tgctcacgaa acatcgcttc
    17881 aacctcgtaa atatgtgttt tagctggaac aaaatgataa tttgtgtact ttccgttttg
    17941 gatgatcaaa tccaacgctc gtttatttgt taaatcaacc ggaccttttt ggctttcttt
    18001 tgctagccaa aacccgatat tacgtaaagt aatcaatgct acaaattttc ctttattgta
    18061 taacggaaat tgtgaatacc ttttttctgc tactatcttt aacagttcaa gtaacggaag
    18121 atcttgttca aaacctgtta cacgttttgc aaatcgaggc agcacggttt caggctgaat
    18181 cagttcttgt tcgatcagct ggattcgctt gactgtccaa tcatttggct ctgcaatgac
    18241 aaaatcgact gcgatccgat catggacgat cgcattcctt aactgagcaa gctgtaaaag
    18301 atcatcttcg tattttctga tcattaattg ttttctttga gctaggcgtc tgacaagttc
    18361 ggtaaatccc atatttcgtg catttcccat ctcgtctctc atccattttt cgattcgatt
    18421 gaaactgatt aaaaaaagat cagagtttgc cataattttc ctccttgatc aaatatgccc
    18481 ggttttttat atagaaaaag aacgcaatca acacaataac tgcaaaaaaa ctgaaaaata
    18541 aattttgtag gaaaacggcc cccaacccag ttgctagaaa taaaaacgga tagatttgaa
    18601 gaatcaagta attcattgtg atcacataag caggctttgc ttctttcact gtataaacgg
    18661 ggctttttag ccaataaaag gtgacctgca tcgtaaaggg cacttccaga tcaagcgtta
    18721 tcttttgttc atgactgaga acactgcgtt tttcattgtt gattcttagc tgaatcgagc
    18781 tacccatacc gtaaaaaccg gtttttctta taaatgtcgt tttcatcata ccctcctatt
    18841 atgctttagt atagctcaaa atacaataaa gaaaaagaat gatcctagtg taaacaaaat
    18901 taattcccta tcgtctaaaa aaagaagctg tagtcaaaat acagctcctt cgatggtaga
    18961 ttataatcaa gcttaaaaca atcccaacac ttctccgtca tttgtcacgt ccatattcaa
    19021 tgcagcaggt tttttaggca aaccaggcat cgtcataaca tctccagtca aggctactaa
    19081 aaatcctgcc cctaattttg ggatgatttc gcggatcgtg atggtgaaat cttccggtgc
    19141 acctagcagt gaaggatcat cagaaaatga atactgtgtt tttgccatac aaatcggcag
    19201 tttgtcccat ccattcttcg taaactcttc tatttgtttt tgcgcttttt tgctgaaaac
    19261 aacaccattt cctccgtaaa ttttcttgac gattgtttct gtcttttctt gtatcgtttc
    19321 ttctaggcgg tataatggct tgtaattagc ttcttttcta tcaatcaatc gtactacagc
    19381 ttttgctaaa tcaacgccgc cttctgctcc atctgcccat acactcgctc tcttggcaag
    19441 aatcccctga ttttcacaca agtgctccaa cagtgtgagt tcattgtctg tatctgttac
    19501 gaattcattg attgcgacga tcacaggtag ctgatattgt tccatattac gtatatggcg
    19561 ctttaaattt gcaaaaccta ctttcaaagc atcaagattc tcatttttaa gttcatcttt
    19621 tttcattccg ccatgcattt tcaatgcacg gacagtggcg acaatcacca cagcatccgg
    19681 cgcttttttc aagttaggta ctttgatatc taaaaatttc tctccgccaa gatctgcacc
    19741 aaatcctgcc tctgtgacaa cgtaatctgc taatctcaat gctgtcttgg tagctaaaat
    19801 actattgcag ccatgagcga tattggcaaa cggtcccccg tggacaaaag ctggtgttcc
    19861 gtaaattgtt tgtacaagat tcggcttgat tgcatctttc agcagtaatg ccagagcgcc
    19921 ttctgctttc aagtctccag cagtcacagg ctgacggtca aatgtatagc cgacaacgat
    19981 ccttgcaaga cgagccttta aatcttcaag gtctgccgca agacacaaga tagccatgat
    20041 ttcgctggca actgtgatat caaacccatc ttctcttgga acaccttgga ttggcccacc
    20101 caagccaaca attactttac gcagttcacg atcattgaga tcgactaccc gtttccaaat
    20161 cactcggcgg gcatcgatgt tcaattcatt tccttgatga atatgattat ctaaaagagc
    20221 agataatgca ttattagctg ttgtgatagc atgcatgtca ccagtaaaat gcaagttgat
    20281 atcttccata ggcaatacct gtgcatatcc tccgcctgca gcaccacctt tgattcccat
    20341 cacaggacca agtgagggtt ctcgcaatgc aataacagat tttttattga ttttattgag
    20401 cgcgtctcct aaccctacag tgatcgttga ctttccttcg ccagctggtg ttggattgat
    20461 ggaagtaact aagatcaaat gcccctcagc gttatccttt gatcgattga ttccagaaaa
    20521 attgattttc gccttatatt tcccatacag ttccaaatcg tcttccttaa gatctaattt
    20581 actagccact tcaacaatcg gttctagctc tgtttcttgt gaaatttgca gatcattctt
    20641 catttgatta ccccttttca cgaactttat tcaacaaaat atcttctttg ttaagatatg
    20701 ccaattatac ctttttttat tcagaaaaaa aacataaaat tacaggtttt taatttattt
    20761 gacgtactta taaatactct ttataatgaa ggctatgatc agactttaga aaggtcgtga
    20821 gtgctttgac cacaggaatt gtaaaatggt ttgacaacaa aaaaggatac ggctttatca
    20881 gttatgatga cacagaagaa atttttgtac attttactgc gattgaagaa gagggattca
    20941 aaactttgga agaaaatcaa gtggtcgaat ttgagatcat cgaagggaat cgaggaacac
    21001 aagctgccca tgtcaagaaa ggcaacgttc agactaccga tactagtatg catgaataga
    21061 taaacaaata gagtgagacg ctaaacggga tgaaccgtac gtctcactct tttcttttcc
    21121 tgtaaaaatt cgttttttta ggttctgatt tttaaaatta gctttttctt tttttcgtat
    21181 aaggataagg aattgattct gatttgggtc cgtttttttc aaaatgatca aaaaggacta
    21241 cttgatcttt ttttcgcatc atgaaaacag atggggcttt ctcatcttgc aacatcattt
    21301 gcaccccagt attagttggc gtcaaagatc gaatctgtcc gacagatatt tctcggtctt
    21361 tccaaaaagc agcataacgt ataatcacag agtttgattt taaaataaaa gaacgccgca
    21421 aacctagaaa tagaaaaaca agaaaaatac ctaaaacaaa attgcttttc caataaggac
    21481 ctgtgttttc taaagaaaga atcagactaa aaaacagaat gatcaaggtc aatgaccaat
    21541 agatgatcgt atgtgccagt tcaggctgcc atcgaatatt ttttttcatt ctctaacctc
    21601 tcaccgattc agtaatgata taatcttacc ataacattag tttgaacaaa aaggagaatc
    21661 tctttttatg ttaaaagtat acattgatgc ctctacaaaa ggcaaccctg gtcctagtgg
    21721 aggtggaatc ttgattgtct atcaaggtaa acaagaacag ctatctatcc cgctttctat
    21781 agggacaaac catcaagcag agttcgaagt ctttttaaaa actttagaat ttctaaagaa
    21841 aaacgactta cagaatgaaa cgattttctg tttttcagac agcaaaacac tagtttctac
    21901 ggtcgacaaa aacgcaacga agaacgagct cttccttccc taccttcaaa gaatccaaga
    21961 acttctatcc gaattttctt tattgatcct gcaatggttg cccgaaaaca aaaataaagg
    22021 agcagataat cttgcacgtc aggctctcca taaacaatta aaagaaaaaa agactcttta
    22081 aattaaaaac tttaaggagt ctttttatag tttctcattt aatataaaag ataattgtga
    22141 tcaaacgtaa tttccaattg gtaatctccg attttttctt ctatttgact acgtaagatt
    22201 ctttctagtt ctgtatcaac aaactggaat ttctcaggga ccactacatc aaattgcagc
    22261 gtttttcctt gatgaattac tctaaaatca tgaatattaa gccctgtacc tgtttcaaga
    22321 agaatttgct ttaactgccg caagatatta tagtattttt cgttatcaac aggcattgga
    22381 tccaagtgac agacaagttc cacatccaaa gactttttaa agtctttttc tatcagatca
    22441 ataatcttat gagccacatt caaattcatt gattcatcta cttcgatgtg gactgatgca
    22501 aagcgtttat tgggtccata gttatgcacc aataaatcat gatagccaag tatatcatcg
    22561 caagcattta aatgctcttc catttcttca atctctgctg gcgttgggcg acttcctaat
    22621 agttcgtata caaaatttcg aagcatcgtc aatccactat aaaggatata taatgctaaa
    22681 gcaaaaccta tatatccatc aatacgccat ccagttaccc attcgattgc tgctgaaagc
    22741 aatacagcca gtgtagtaaa gacatcatta taactatctg ttgctgtagc tctcaatgtt
    22801 tcagaatcaa tggattgcgc aataacctga tacattttgc cttgtgcaaa tttcaaaaag
    22861 atggaaatga ttaggacaaa aaagacgatg ggtgttaaag agacattttc tggatggatg
    22921 attttttcag cagaagactc taaaaattga aatcctacat aagtaatgat gatcgaaaca
    22981 aataacccac tgatatattc gaagcgttcg tgtccataag gatgttcctg atctgctggc
    23041 tttgcagcaa ttttaaatcc gatcaaagtc aatatggatg aagcagtatc tgatagactg
    23101 ttcactgcat cagccataat ggagacactt cctgaaaaaa gcccgatcat tagcttagag
    23161 acaaacaaga tcagattcgt aaccaatcct aagatacccg ctagtactcc tttttttgtc
    23221 cgctgttgcg gtatttgttt ctttttttct tcttgtttct tagccaagtt ttttacccct
    23281 tctagtttgc ttgttgctca ggtaaaaaga tggagaggac gattgttttc ctctcctctc
    23341 ttcctttttc tatctatcgt gcgtcctatt actattaaaa gatggtcatt attctggttt
    23401 caacggtgtt gctgcaaata acgcttttgc tagtaatggt gttatgattc ccgcacctat
    23461 tgcttctggt atcccattga ttgctgcaac ccctgctaag gttttaaata aaagagcaga
    23521 gtctacacca taggcatctg ccgttgcacc tgtataaaat aatcccatga atcctaacac
    23581 taagactgta ttgaccaatg aacccagtac ccctgcaaag gctgagctga tcgttattga
    23641 tttatttttt tgataaatcc agtggaaaac caagcctgct actaagccaa caagtactct
    23701 gggaactacc gagataatcg gattcgtgaa cactaatgta tcaaatggcg ttgttggcat
    23761 ggcataggca cgaacaattg tagcgattcc ccaaaacaag ccaatcacca tgccatcttt
    23821 tgggcctaat actactgcag caataattac tgtgatatgg ataattgtta aactaatgaa
    23881 tcctaatgga ataaacccca gccaaggaac catcgcttgt acgatgatga ttgctaacaa
    23941 gatcgcacgt aagactagtc gaaaggtctt atttctacta ttcatttttt cacccctttt
    24001 tatttatact aatcgtaaaa atttctgtca ctttcgtcaa ctaaagacca tccgaatccg
    24061 accatttgat tagatgcgcc atccaattga aaaaagtatg agggcaagac ttgtttgtct
    24121 tcgcccccat aatcatgttt cgttcaatgt ttgaactttt gtttttctgt caaacgtaaa
    24181 cgccctattg ttgcgcaacc cattctttcg cagctttaag tgcatctgga ataccagcag
    24241 gatttttccc gccagcttgt gccatgtctg gacgtccacc accgccaccg cctactttcg
    24301 gtgcgattgc tttaatcaag tcgccagctt ttagtccagc ttcatttgat tctttcgtca
    24361 ttgccgctag cagactgact tttccttctt gttcgtttgc taaaacaaga atatctgaaa
    24421 tatttttttg tttccattgg tcagctaatt gacgcagctg attcatatct ttcactttta
    24481 cttgtgctgc aatgattgtt tttccattga tcgtttctgg atctttgaat acatcaccag
    24541 cttgttgatt cgctaacttg ctagctaatt gttcattttc cttttgtagc tctcttactt
    24601 gctgttgcaa ttgttccgct tttgaaacaa cttctttcaa ttgaggtgat ttaacaattg
    24661 ttgctacttg ttttagttgt ttttcttctt cttcaagtag ttcgtacgct tctttacttg
    24721 ttacagcctc gattcgtcgc acgcctgcac caattcctga ttcagaaacg attttgaaga
    24781 ttccgatgtt ttctgtattt gctacatgtg tgcctccgca cagttcgatt gaccagccgc
    24841 caatatttac tactcgaacg tcatgtccgt atttttcacc aaacaaagcc attgcaccca
    24901 tgtttttggc tgtatcgata tctgtttcaa ttgtttcaac tgggatagct tcccagattt
    24961 tttcgttgac gattctttcc atttcagcta attcatctga agtgatttga ccaaagtggg
    25021 taaagtcgaa tcgtaaatgt ccaggagcga ctaaagaacc agcttgattt gcatgctcgc
    25081 caagtacttc tttcaatgcc ttgtgcagta gatgggttgc tgtatggttt ttgatgatcc
    25141 gattgcgcat cgattgatcg atcaatagat gataggtttg tccttcactc attggtgctt
    25201 ttacttctac ggtgtgcata aattgaccat taggcgcttt ttgaacatct gtaacaactg
    25261 ctacgatttc gcctacttca ttcatgatcc atccttgatc cgctacttgc ccacccattt
    25321 ctgcatagaa aggtgtttga tcaaagatca tttgagcttc accttctgcg acttcattca
    25381 aaagagactc acctttaacg atgaccaata attgactatc tgattcttgt tctgtgtatc
    25441 caatgaattt actttctact ttgatatctg tcaataaagc agattgtacc cccattgatt
    25501 tttcagtact gcgggcagag cgagcacgtt cgcgttgtgc atccatttct ttttcaaaac
    25561 cagcatgatc aactttcagt ccttcatctt ctgccacctc ttccgttaat tctaccggga
    25621 agccatatgt gtcgtatagt ttgaagatgt cttttcctgc tagagtcgtc ccgttttcgt
    25681 tttttacttc ttggatcact tgatttaaga tttccaaacc ttcattgatt gtttcattga
    25741 aacgttcttc ttctgtacgg ataacttttt caataaattc tttttgttga agtacttcag
    25801 gataataact taccatgatt tccccaacaa cagggactaa acgatataag aatgcttgtt
    25861 caatacctaa ttttttccca tgcatgacag cacgacgaag caaacggcgt aagacatacc
    25921 ctctcccttc attcgatggc aatgcaccgt cgccaatagc aaatgaaagg gcgcgaatat
    25981 gatctgcgat tactttgtaa gaaatatctg ttactgggtt ttcaccatat tttacttgtt
    26041 tgcttagctt ttctacggcg tggataatag gcataaacaa gtctgtctca aagttcgttg
    26101 acgcatcttg aacgattgag accatgcgtt ccaatcccat ccccgtatca atgtttttat
    26161 gaggaagtgg ttcaaatgtg ttatcaggct tatggttgaa ttctgaaaat actaagttcc
    26221 agatttctaa ataacgttca ttttctcctc ctggatagtt ttcaggatcg tcttctgcta
    26281 aatcgttata agcttctcca cggtcataga aaatttccgt atccggacca catgggcctg
    26341 caccgatatc ccagaagttg tcttctacat caataatgtg atctagtggc aggccaactt
    26401 cttcatgcca gatgcgtttt gcttctgtat cttttggata aactgttaca taaagttttt
    26461 ctggatcgaa tgccatccat tttggatcag tcaaaaattc ccatgcccag tggatggctt
    26521 catttttgaa atagtcacca atcgaaaaat tacccagcat ctcaaacatc gtatggtgcc
    26581 gtgctgtttt accgacattt tcgatatcat ttgttcgaat agatttttgg gcatttgtga
    26641 ttcttggatt ttctggcaca actgaaccat caaaatattt tttcaatgtt gcaacaccag
    26701 agttgatcca caataaggtt ggatcgttca cggggatcaa agacgcacta ggttcaatcg
    26761 tatgtccttt agattggaag aaatcaagaa acatttgtcg aacttgtgca ctacttaatt
    26821 gtttcatgtc attacctgct ttctataaat taaatacaaa aaaacaccat cacagccaag
    26881 gacgaaaact cgcggtacca ccttgattgc aatgatgtgt aatcattacc tcttaaactc
    26941 gataacgctg agctgcgtcg gtatttctac caaaatagaa aaagggagca atttgattag
    27001 tgactattct tttttcagcc aagaaagaat tttctgtgag tcttaaaaaa caaatcatat
    27061 ccttttcgta cttaattagt atacgcattc tattgatcac tgtcaatatc tcaattttta
    27121 ttttcatttc cttttggtca tactccacga tttttggtac gataagaata aaggaatcaa
    27181 tacatctctg ctattcatga caaggaggaa tatgatgaaa aaaatagttg gagcggatga
    27241 atggatacgc gcagctagct gctttttgag atatcaagtt tttgtattag aacaagggat
    27301 tcctgcagaa ctagaattcg acggaaaaga tatcccgcaa caaacctatt atctatttct
    27361 tgatcaaaaa agaccagtag ctactttgcg ttatgaaaag aaaacaccta cttgcttcaa
    27421 gccggaacga ttttgcgtag caaaagaata ccgaggaaaa gggttaggcg gtcagttgct
    27481 tgaagctgct gaaaaacggg cggtttcaga aggttgtcag actagttatg tagtagctga
    27541 aatgacggct gtagattttt atgaaaaaca agggtatcat acttgcacag aaccttttat
    27601 tgaagatgaa gtactttgta tcggaatgaa aaaaagtcta gcttaaaaac aagagattgt
    27661 gacacgcctt ttgtcacaac ctcttgtttt ttatttatgt gaatttttct tatttttacg
    27721 agcagtacgg ctttgctgtc tgcgttcgat tttgcgttct tttttatttt tttctgcaat
    27781 tgcccaatcg atttttttct tataaccagg cttgatcttt ttctttttct tcttcacaag
    27841 accaatcagc gtgggatcta atacatcttt tgatttttct cgtttggcac gtctattacg
    27901 atcgtatgtt ttgacgattt ccccgttttt gatttctttt ggttcaaatg agacgcccaa
    27961 tttttctatc gcatcaatgg cttgctcatc atttggcgaa tataacgtaa tggccgttcc
    28021 ttttaagcca ttacgtccag tacgtccgac acgatggata aagaagtcta gatcttctgg
    28081 aatttctgca ttaataacat gagagacacc ttcgatatcg atccctctag cagctaaatc
    28141 agtcgctaca acatattgat actccagatt ctgtacttgt cgcatcactc gttttctctc
    28201 tcttggggtg atgtctccat gaatctttgc tactttcaag ccttggtttt tcaagtaatc
    28261 ggcaatttca tccacatgct gttttgtatt ggcaaatacg attgctaaat aaggatgacc
    28321 aattgtcagc agttgataaa tcatttgatt ctcatcttta ccttttgttg aaatcagcca
    28381 attttcaatg tcttcagaaa taactgcttt tggtttgatt tcttcaatga ttggattttc
    28441 catgtatttt cgtaagaatg gacgaagttt ttctggaata gtggcagaaa aaactaaaaa
    28501 ctgcagcttt tctggtaatc gacttgcaat ttggtctact tcatttaaaa aacccatatc
    28561 caatgtcata tccgcttcat cgacaacaaa tgcaaatgcg gtgtgaatct tcagcgcttg
    28621 ttcattcatc atgtctaata ttctaccagg cgtgcctact acaacctgtg gctgttggtg
    28681 atgcagacga tttaattgac gctgtttgtc tgtaccgccg acaaaattcg agacacggat
    28741 ctccggtgta gaaaatttag cgatttgtgt agctgcttca tagatctgtg cagctagttc
    28801 cctacttgga gctgtaataa caacttgtac ttcctctttt tctgcattga ttttttcaaa
    28861 caaaggcaac aaaaacgtat gtgtttttcc tgtaccagtt tgagattgcc caatgatatt
    28921 ttttcctttt gcaattaaag gaatcaaacg ttcctggacc tctgtgggct gtttgaagcc
    28981 ctttgcggtt aacgcttcat tgataaacgg tttgaagcgg aatatatcaa aattattcat
    29041 tcgtgcacct cttctcttta aattatcacg actttggcat tataacacag acaaagcgtt
    29101 tttgctcagc taatttcgag aatcatcatc ctttgtttgg ccaatcgtgc taacagtata
    29161 tgtgtgacta gctttttcat aaaacagaat ccgttttttt ctcaaaaatg atagcaagat
    29221 atttaaaata aaaatacctt gtagtaacaa gacaccgaga gaaatgaata ctgcactcat
    29281 ctgcaacacc gtattcgttg tcgaattcat gatttgctga agcaactgcc ccaaccagct
    29341 taaaccataa aaaagaaagt acagataccc gtagcggatc atcaatgctt ttaaagaaat
    29401 tcgttcgcgg tcttcttcca ccaaacggat acgcacaatt tttttgccaa gtgtccatcc
    29461 attggttata aacggtacaa tcataaagta tacccctact tctaataaaa gccaccagat
    29521 atgagaagta actgcccctg aatcgaacgc aattttaaag aaaatcgccg ctaccaaatt
    29581 aataagagat aaaaatgtcc agtcgatcaa ataagcgacc aagcgtcgag tcaatgagac
    29641 agtctttcct ttttcataag aaagttcatc cattcgtctt cttgtaggaa gcataaatgt
    29701 aaataacggc gtaacgacaa aaccaatgat tccacccaat gtatttgtca ctaaatcatt
    29761 cacatcaaat aagcggtatg gtcttgggta gataaaataa agtccactta gctgcgtcaa
    29821 ttcaaaaaat agagaaaggc agaaactagc aatcatcgtt ttcataaaag aaagccggaa
    29881 ataatatctt aaataaatgc caaacggtat gagtaataaa acattaaaag ctggttccaa
    29941 aaatacactc tgcttcattg ccggcaggta ggtactaggt tcagacaaga ccaataccgt
    30001 gttgtctctg aaatgttgcc atgaagcaaa taaatgcagc tccatctttg gccctgtcat
    30061 ttgggcaacc gtctcctttg ccggaagtgg taagatcacc aaaaaatacg cacacaataa
    30121 ataaaaaacg aaagaataca aaataactgc ccttgttact agaaatgttc catactttcg
    30181 atattcataa ataaaaaaaa cagatgagat cgctaaggct aaaaatggaa aaacaatcag
    30241 cgcaaaacga atcggttcga tataacttgc catatgactc ctccttgatt ctttgtatct
    30301 ctcttatctt agcagtcctt tgctgtttca agcaagtaac gaggctatta tttctcattt
    30361 ctttcaaagt aaagtaagat aaacacaaag ggggtgtcga gatgctgaat atgtactttg
    30421 tttttggcgt acctattttt ttactgttcc tttatgctac gatcgcttat gtaagaaaaa
    30481 gaactacaat tcattattta ggatttattc tcctgattat ttcgggtttc atgctagtct
    30541 tcaatcttca gacttggcag caagcacttc ttgaaatgga taaaatgact ccacatgctt
    30601 tgtctaaagt gcttggctac cctgtctatc tgatttggct tcccattttc atttctggtt
    30661 gcttggtttt attaaatatt tatcgcggcg tacgccgaat agttcagtta cgcaaatcaa
    30721 agtaaaagac agaaatcggt tgagcccgat ttctgtcttt tactttgcta tttcatcgta
    30781 gacaaaacga catttacgtg atcaaaatta acgagtactt ttgacatttc ttccacgtac
    30841 tcctcattta aataaaatat tttgtttggc agagaagtgc agttctaata aaattcgaat
    30901 gttttagcta tctacgtttt aaatagagat aaatcgggat accagcaagt gtcaatacta
    30961 tccccatcga agctaaaaag gtctgagtga taagcgtgct tgatacgata aaagtccctc
    31021 caatgattgc tactagtgga ataaacggat acattggaac tttgtatggt cgaaacaaat
    31081 caggttcttt tttacgaaga agaatgacac caacaaagac catcgtataa aaaatccaaa
    31141 tgacaaagat cagcatatcc gttagtgtat caaaaccacc gatcatcatc atgccaatag
    31201 cgatgactag ctctaagata cctgcggcta caggaacttt agttttatca tgaagtttag
    31261 caaagagctt gctaaaaggc aggttgtttt cttttgccat aacataaggg agacgcattc
    31321 cagtaagggt atagccattg atggttccat aaacagaaat caagatacca actgtgacca
    31381 gtcttccacc gaagccgcca aaaatcattt ttgcaacgtc acttgccgca ttgctgtttc
    31441 ctgcgactcc gtcgatagat gcggttctta agaacaccgc atttactaat aaataaacga
    31501 tcatgattcc gatgattcct aaagaaattg cttttggcaa atctttcgcc ggctttttca
    31561 attctcccga aatatttcct acgtgtatcc aaccgtcgta tgcgaacatc gttgctagca
    31621 agcccgctcc caaggctgag aaaaatgaaa gattttctcc tgcttggatc ggaaacagtt
    31681 ggaagtctac tccttcttgt cggaacaaac cgaaaatgac gatcacaaac aaaggaataa
    31741 gcttgcagac aagcgtaatc gactgaaaag cccctccagc tttagaacct aaaaaattga
    31801 tcaacaggat cgatacagca gctgtaacag ctacgggaac aatcattgac tgggaaagac
    31861 caaatagatt gacgaactgt gtaccgaaaa tgatcgacaa ggctgctaca tttgctggaa
    31921 aataaatcac gacttgcgcc cagcccaata aaaaagctgc tgtatttccg taaatccgtt
    31981 cgatatattt aatcatccct cctgtttctg gaatagcagc tgccagttca gctcctgtta
    32041 gtcctgcaca gacagaaatc ataccaccga gaaaccaaga aaacatatgc aaactggcgg
    32101 atcctgtaac ttctgctacg cttgcggctt taaaaaatac ccctgcaccg atcactgtgc
    32161 ccataacagt cgataaagca gtaaaaaatc ccatcgtacg ttttaattca ttttctattt
    32221 ttacttcttt tactggcgtg gcttgatatt cttccatccc ttgtcccctt tcataccata
    32281 gccaatccca aaagtataaa aaactttttg gatgtggttc ttgttttata ccgtctctac
    32341 caatttttct ttcagttttt ccaacatatc ctttgtcatc acatctaagt cgtatttcgg
    32401 cgaaaagccc cattcctctt gcgcacaaga aacatctagt gaatttggcc aagattctgc
    32461 tattgactga cgtacaggat ccacatcata atccatttca aaatctggta tgattttttg
    32521 aatagatgct ttgatctctt ccgggtcaaa agacatcgcc gatatattga aggcatttcg
    32581 atgaaccaat cgatcaggat ctgcattcat gagttttata atggcatcga tcgcatctgg
    32641 catatacatc atatccattg atgtaccttg atcaatgaaa ctagtgtact ttccttcttt
    32701 gattgctgaa taataaatat cgactgcgta gtctgtcgta ccgcctcctg gcaaggttgc
    32761 ataagaaatc aatccaggga aacggacacc acgagtatct acgccatatt tcacataata
    32821 ataatcacat agtagttctc cagctacctt cgttacccca tacatggttg taggacgctg
    32881 aagagtatct tgtggggtat gatcttttgg agtatttgga ccaaacgcgc cgattgaact
    32941 tggcgtaaaa aatttcagct ggaattctct agcgacttcc aatgcattga ccagcccagt
    33001 cacgttcaaa ttccaggcta attgtggttt tgcttctgca acagctgaca aaagagcagc
    33061 caaatgaatc aatgtgtcca cttgatattg ttctgctatg ctgcgcattt tttcgtaatc
    33121 cattacatct aggatctcaa agatatcgtt tttctcaatg acttcaggca tgcgtatatc
    33181 tgtcgcaatg acattttcac tgcctagttc ttgacgtagc cgtgtgatca actctgaacc
    33241 aatttgtcct aaacatcctg tcaccaatac ttttttcatg atgatcctcc ctgatctttt
    33301 ttactactat cttcagatga ttaacaatat atgcgtgaac atgttctcta attttaaata
    33361 gacgaaaaaa ctaaatttag ttaaataatt tccatttctt ttcctatttt ttgataaact
    33421 gctactgctt catcaagcat ttccctcgta tgttccgcag tcggcatatt tctgatcctg
    33481 cctgtcccaa gaggcacagt tggataaacg attggtttag cataaatccc atgttcgatc
    33541 agcttcttag agaaagtctg agtcactttt tcgtctccca aaatgactgg agtaataggt
    33601 gtttcagaca taccgatgtt atagcctact tttttcaatt cttctttaaa ataattagca
    33661 ttttcccaaa ctttttcgac aagttccgga tgttcttgca tcaaagtaat cgaagccaat
    33721 gcagctgcag ctgcacctgg agttagcgaa gtagaaaata ggaatggccg acttctagct
    33781 ttcaaccagt cgattagtgt tttcgatcca gctacataac ccccaacaac acctatagct
    33841 ttcgataacg tgcccatttg catatcgatt ttgtcgctta atccaaaatg cttgacggtt
    33901 cctgcacctt ttccagttac gcctgagccg tgcgcatcat cgacgtaggt gatcagtcca
    33961 tatttttcaa caatcggaat aatttccggc aatcgtgcaa tgtctccatc catggagaaa
    34021 acaccatctg tgatatacat gattttttta tatttcttac tttctatcgc ttcctttgct
    34081 tttttctcaa ggtctttcat atcttgatgt ttaattcgga tgatttttgc tccggataag
    34141 cggcaaccgt caataataga tgcatggttt aattcatccg ataagatagc atcttctttt
    34201 gtcattactg cagagatagc gcccatattg cagttgaatc cagattgaaa ggcgattgct
    34261 gcttctgtcc ctttgaattc tgcgattttt tcttctaatt gctgatggat tttcagcgaa
    34321 ccattgatcg ttcggactgc tcctgctcct acaccataag tttcggttgc ttcaatacat
    34381 gcttttttca attcttcgcg attggcaaat ccaagataat tgttggatgc tagattgatc
    34441 atcttttttc cgtctacgat gatacaagca ccattttcac tttctagtat gtcgatcgta
    34501 ttgtataacc ccttttcttt cagttgatcc aattctttct ctaaatacat tgttagttct
    34561 ttggaagaca ttgtctccac ctccatattc atctgtcttg cagaacatac caaacatata
    34621 tcgtgtcatt catctacaaa gcaagtatgc tatatttttg ataacgctta cactgattat
    34681 gagaattatt taaccacatt ttttatttac agtatctttt tactttcaaa tgacttatat
    34741 agtatgaaca attacctttt agataatgat ctattttact ctacctgggc agataaatac
    34801 aacacaacca ttgatagaag ccctttcgtt aaaatcaatt gtattctgaa tatttaattt
    34861 atttattgca tagaccaata aaaatacata aaatttccat cttcgtttat cagcaatttt
    34921 taattttact ctctgaatat ttcttaaaag ttttacttgt tgaattcgaa aaaaggactg
    34981 tgacaaaagt cttgtcacag tcctattctt ccgaata
//
LOCUS       DAZKPO010000022        34687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000022 DAZKPO010000000
VERSION     DAZKPO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.022"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG8NZ_001045"
     CDS             591..1280
                     /locus_tag="ACG8NZ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0538739.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG8NZ_001046"
     CDS             1267..2475
                     /locus_tag="ACG8NZ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0538740.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG8NZ_001047"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG8NZ_001047"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0538741.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8NZ_001048"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8NZ_001048"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0538742.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG8NZ_001049"
     CDS             complement(6578..7273)
                     /locus_tag="ACG8NZ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0538743.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8NZ_001050"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8NZ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0538744.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG8NZ_001051"
     CDS             complement(8472..8663)
                     /locus_tag="ACG8NZ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0538745.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG8NZ_001052"
     CDS             complement(8684..9574)
                     /locus_tag="ACG8NZ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0538746.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG8NZ_001053"
     CDS             complement(9561..10328)
                     /locus_tag="ACG8NZ_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0538747.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8NZ_001054"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8NZ_001054"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0538748.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8NZ_001055"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8NZ_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0538749.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8NZ_001056"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8NZ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0538750.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG8NZ_001057"
     CDS             14899..15282
                     /locus_tag="ACG8NZ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538751.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8NZ_001058"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8NZ_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0538752.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG8NZ_001059"
     CDS             complement(16226..17044)
                     /locus_tag="ACG8NZ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0538753.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8NZ_001060"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8NZ_001060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0538754.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8NZ_001061"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8NZ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0538755.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8NZ_001062"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8NZ_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0538756.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8NZ_001063"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8NZ_001063"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0538757.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8NZ_001064"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8NZ_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0538758.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACG8NZ_001065"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACG8NZ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0538759.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8NZ_001066"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8NZ_001066"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0538760.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8NZ_001067"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8NZ_001067"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0538761.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8NZ_001068"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8NZ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0538762.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8NZ_001069"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8NZ_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0538763.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8NZ_001070"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8NZ_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0538764.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACG8NZ_001071"
     CDS             28352..30325
                     /locus_tag="ACG8NZ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0538765.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8NZ_001072"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8NZ_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0538766.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8NZ_001073"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8NZ_001073"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0538767.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACG8NZ_001074"
     CDS             33204..34496
                     /locus_tag="ACG8NZ_001074"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0538768.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10569 a         6743 c         6858 g        10517 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaa
//
LOCUS       DAZKPO010000023        34560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000023 DAZKPO010000000
VERSION     DAZKPO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.023"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..2135)
                     /locus_tag="ACG8NZ_001075"
     CDS             complement(243..2135)
                     /locus_tag="ACG8NZ_001075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538769.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2132..3859)
                     /locus_tag="ACG8NZ_001076"
     CDS             complement(2132..3859)
                     /locus_tag="ACG8NZ_001076"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538770.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3875..4525)
                     /locus_tag="ACG8NZ_001077"
     CDS             complement(3875..4525)
                     /locus_tag="ACG8NZ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0538771.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4729..5025
                     /locus_tag="ACG8NZ_001078"
     CDS             4729..5025
                     /locus_tag="ACG8NZ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0538772.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5197..5817
                     /locus_tag="ACG8NZ_001079"
     CDS             5197..5817
                     /locus_tag="ACG8NZ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0538773.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5909..7075)
                     /locus_tag="ACG8NZ_001080"
     CDS             complement(5909..7075)
                     /locus_tag="ACG8NZ_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0538774.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7087..7440)
                     /locus_tag="ACG8NZ_001081"
     CDS             complement(7087..7440)
                     /locus_tag="ACG8NZ_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0538775.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7568..8590
                     /locus_tag="ACG8NZ_001082"
     CDS             7568..8590
                     /locus_tag="ACG8NZ_001082"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0538776.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8609..8770
                     /locus_tag="ACG8NZ_001083"
     CDS             8609..8770
                     /locus_tag="ACG8NZ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538777.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9029..9463)
                     /locus_tag="ACG8NZ_001084"
     CDS             complement(9029..9463)
                     /locus_tag="ACG8NZ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538778.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9573..11432)
                     /locus_tag="ACG8NZ_001085"
     CDS             complement(9573..11432)
                     /locus_tag="ACG8NZ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0538779.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11433..11573
                     /locus_tag="ACG8NZ_001086"
     CDS             11433..11573
                     /locus_tag="ACG8NZ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0538780.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11550..12710)
                     /locus_tag="ACG8NZ_001087"
     CDS             complement(11550..12710)
                     /locus_tag="ACG8NZ_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0538781.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13017..14441)
                     /gene="araA"
                     /locus_tag="ACG8NZ_001088"
     CDS             complement(13017..14441)
                     /gene="araA"
                     /locus_tag="ACG8NZ_001088"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0538782.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14457..15155)
                     /locus_tag="ACG8NZ_001089"
     CDS             complement(14457..15155)
                     /locus_tag="ACG8NZ_001089"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0538783.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15112..16773)
                     /locus_tag="ACG8NZ_001090"
     CDS             complement(15112..16773)
                     /locus_tag="ACG8NZ_001090"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0538784.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16991..18391
                     /locus_tag="ACG8NZ_001091"
     CDS             16991..18391
                     /locus_tag="ACG8NZ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0538785.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18643..19722)
                     /locus_tag="ACG8NZ_001092"
     CDS             complement(18643..19722)
                     /locus_tag="ACG8NZ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0538786.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19808..20767)
                     /locus_tag="ACG8NZ_001093"
     CDS             complement(19808..20767)
                     /locus_tag="ACG8NZ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0538787.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20779..22290)
                     /locus_tag="ACG8NZ_001094"
     CDS             complement(20779..22290)
                     /locus_tag="ACG8NZ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0538788.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22312..22875)
                     /locus_tag="ACG8NZ_001095"
     CDS             complement(22312..22875)
                     /locus_tag="ACG8NZ_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0538789.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22881..23744)
                     /locus_tag="ACG8NZ_001096"
     CDS             complement(22881..23744)
                     /locus_tag="ACG8NZ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0538790.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23758..24654)
                     /locus_tag="ACG8NZ_001097"
     CDS             complement(23758..24654)
                     /locus_tag="ACG8NZ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0538791.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24829..26118)
                     /locus_tag="ACG8NZ_001098"
     CDS             complement(24829..26118)
                     /locus_tag="ACG8NZ_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0538792.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26090..27607)
                     /locus_tag="ACG8NZ_001099"
     CDS             complement(26090..27607)
                     /locus_tag="ACG8NZ_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0538793.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27805..28695
                     /locus_tag="ACG8NZ_001100"
     CDS             27805..28695
                     /locus_tag="ACG8NZ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0538794.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28768..29592)
                     /gene="nadE"
                     /locus_tag="ACG8NZ_001101"
     CDS             complement(28768..29592)
                     /gene="nadE"
                     /locus_tag="ACG8NZ_001101"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0538795.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29604..31076)
                     /locus_tag="ACG8NZ_001102"
     CDS             complement(29604..31076)
                     /locus_tag="ACG8NZ_001102"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0538796.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31713..32075)
                     /locus_tag="ACG8NZ_001103"
     CDS             complement(31713..32075)
                     /locus_tag="ACG8NZ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0538797.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32298..33050
                     /locus_tag="ACG8NZ_001104"
     CDS             32298..33050
                     /locus_tag="ACG8NZ_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0538798.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33190..34113)
                     /locus_tag="ACG8NZ_001105"
     CDS             complement(33190..34113)
                     /locus_tag="ACG8NZ_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0538799.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34128..34400)
                     /locus_tag="ACG8NZ_001106"
     CDS             complement(34128..34400)
                     /locus_tag="ACG8NZ_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538800.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10198 a         7200 c         5951 g        11211 t
ORIGIN      
        1 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
       61 ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa tagcttctca
      121 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg tttattcgga
      181 aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa atttgtacta
      241 ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata gaatccgtct
      301 ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt catcacaata
      361 atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact tgtccggttt
      421 tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc tacactagat
      481 gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc aattgtgatt
      541 agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt gttatatcct
      601 tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc aatgatttgt
      661 tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc tgtaaacagc
      721 caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg
      781 gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg ttcaagaagg
      841 ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt
      901 ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc gaattgaaca
      961 ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga tttttcattg
     1021 gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac tgttgattgg
     1081 atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt tgtatattgc
     1141 aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac tccgcctagt
     1201 acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat gatgattgcc
     1261 gaaataaatt gagctttcca accagcggca tacagattct gattttcttt ttcaaatact
     1321 tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc atatgtttct
     1381 tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat
     1441 ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa cgcgactaac
     1501 gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt gactgcacta
     1561 gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat atcattgatt
     1621 gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg tacacgattc
     1681 attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac acgtgtcata
     1741 ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat tactgtcgca
     1801 atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg gccttgttgc
     1861 atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt ccccaagacc
     1921 tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac agcgatgatc
     1981 gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca gaaattttta
     2041 ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg accgcctgct
     2101 ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct cttagttgtg
     2161 aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc
     2221 caaccagttt tccttcatct agcacaagga tcatatctgc atccattact gtacttactc
     2281 gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct tggcgaagat
     2341 tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag atatctgctg
     2401 ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag aagtttcctc
     2461 ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa tctttagctt
     2521 gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta ccatactgca
     2581 tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag cccattaatt
     2641 ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg atcgaaccag
     2701 attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct gaaccagtcc
     2761 cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct tccaatgcaa
     2821 gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt gcgttttttc
     2881 cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt tcatccaaca
     2941 cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa atagcggaaa
     3001 gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc agattaccga
     3061 cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct acgtttgtac
     3121 cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta actttgattg
     3181 ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat
     3241 cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg tttaaacggt
     3301 cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt ccgccgatca
     3361 aaataatcat tactgggatg acaactaaga agatttttgt caactgatga tctttttgat
     3421 aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca
     3481 tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc aaagaggccg
     3541 ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag acatccgtac
     3601 gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt gcgatagcag
     3661 ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa atataatcga
     3721 catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc aaatataaat
     3781 ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc aaggagatcc
     3841 gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg tttttcagcc
     3901 aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct tcagatgttg
     3961 aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact aattggatca
     4021 gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc aattcaagca
     4081 actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt tggcgactcg
     4141 tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag ttacgataaa
     4201 aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag gattcgaatc
     4261 ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta gagctgtcac
     4321 gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa tactgataga
     4381 aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct tcttttaagg
     4441 gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt tctttaggca
     4501 aatggaaaaa tgtctgtgtc ggcatatgga tcctcctttc tttcaagatg acacattgtc
     4561 atatgacgct gtgtcattat aacacctcct acctgttttg caagacattt gcatagtttt
     4621 tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat ttgaattaat
     4681 gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat ggcccaatca
     4741 attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt tttagataca
     4801 agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat ccccgctgct
     4861 atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat tctaagctat
     4921 acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt tcgtgctgtt
     4981 tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt gcttgttaga
     5041 ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct gtcttttttt
     5101 agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt tctcatagtt
     5161 ataacaatca tagcaataaa aggagaaggt aagcatatgg catctgtttt agtagtcaaa
     5221 ggtcatcccc taactgcaga agaatcgcgc acagttaagg cattgacgtc ttttttaaca
     5281 agttataaag aaaatcatcc agatgatgaa gtgactgttt tggaacttta tcgcgatgat
     5341 attccggaaa tcgatgaaga attattatca ggttgggaag cattacgtgc tggtgcagaa
     5401 ttcacttcct tatcagaaag tcaacaacaa aaaattgctc gtttcaatga attgactgat
     5461 cagttccttg ctgctgataa aatcgttatc gcaaacgcct tatggaatct aaatatccca
     5521 acaaaattaa aagcttggtt cgataccgtc aatgtagctg gaaaaacgtt ccgctataca
     5581 gaaaacgggc cagaaggatt agtgacaggt aaaaaagcac tgcacatcca atctaatggc
     5641 ggtgtataca acggacaaga ctttgcctct caatatgtaa aaggtatttt aaactttgta
     5701 ggcatcgatc aagtagatca gctattcatc gaaggaatcg attatgaccc tgaccgtgca
     5761 gatgagttga tgcaaaatgc attagataaa gctgctgctt taggtaaatc attttaaaaa
     5821 ttgagataaa tgcatacaaa agagacagag gaaaaaatct tcaaagcagc atttgtacct
     5881 tggatatttt cccatctgtc tctttttgtt attctttctg ctttttcaac ggattttctt
     5941 cataaagaga agagattgtt gtgtaccatt caaaaacatg agtaatcact actttagctg
     6001 ctgcataaac aggaatcccc aagatcacac cgatgattcc gaacaatttc ccagaagtca
     6061 gcagaactaa taaaatcgtt actggatgga ttgctaactg attccctagt actaatggag
     6121 agatcaagcg tccttctatt gtctgctcta ctgcaaaaac gatcagtact ttgatgatca
     6181 tgaatggacc tgctacaatg gctaaaaaga tagccggaat catcgctaaa aatgatccta
     6241 agtaagggat caggttcaaa aaacccgcaa taatccccaa cgtgacagca tactctaaac
     6301 caatgaccgc aaaaccaatc ataaacatga ttgccacagc aaaagccaca gttagttgtc
     6361 ctcttatata agaagaaact tgactattca tttcactcag cacattcaac gtcggttttt
     6421 gcatgcgtgt cggtaaaaat ttgacaaaat aaggtgctaa ttgtttaccg tctttcaata
     6481 gataaaataa gataaacggc atcgtcagca gagccactaa aatagtcgct accgcgccga
     6541 caaaacttcc aagactctga actgtactct tagatatatc ttggatcata tcgcctgccg
     6601 aattcatgaa cttatcacct gctgtttcca actgttcgcg aaattggttg aacagcgggt
     6661 cccgcaaaat ctcggttaat ttattatcaa tcgtatctac atattgaggg aagttactga
     6721 tgaagctcac tgtttgttcc tgaatcttag ggatgatcac tacgctcccc catgcgatta
     6781 gtgcaacaac caaaacaaac agcccaatga tactgtaaag ccttggtact tttcgctttt
     6841 caaagtaatc aactaccgga ttcatcaagt aatacaagat tcctgctaaa atgataggca
     6901 gacctacgat tgctaaaaat tgccagattg gtgcaaacaa atatgaaact tttgtaaata
     6961 aaaagacgat cagcaatatt aacaatacaa caagtaaggc tgtgactact tgattattta
     7021 aaaaccagcg ccaaaaccac gataccggtc tgttcttctt attttttcct tccataattc
     7081 ctcctgttag ttataaaaaa tttggcttcc caaaccaata gcaggcagtc tatgcggatg
     7141 aagatagatt ccatttgcaa tttgtccttc ttctggacaa gcatcaaaaa tcaatgatac
     7201 gtgagctact gtctctaaat tcccattggc catcggaccg acataatcga tcgtatatac
     7261 ctgatcatca aaagcaattg ttccgcctgc tttcaaagaa aaaggttcgt cttttgatac
     7321 ttcttgaatc accgaatatt ttcgtaaggc agctgttgct gttttatcga acaaaatcag
     7381 catgggttct tccttgttta ttgcttctgt cccaatttct gtaatctttg cctgaatcat
     7441 tgcactctct cctttaattg ctttcaaaaa cagtataaca cattctcatt ctttagccgt
     7501 gtggaataat tatgtgatag aataaaaacg aaaactattt cattacaatt aggaggaatc
     7561 agctcatatg atcgaaaaga taattttagg aacctatacg cgtcgcgaaa gtaaaggaat
     7621 ctatacgatc gaccttgata cagaaaaaga agaattagcc aacctttcat tagctgttga
     7681 agaaaatagc cctacctatg tagcaaaaag taaagctggc aatctttaca cagttactag
     7741 tgtggatgga ttaggtggtg ccggtgcgta cgataaggat tttcattttt tgaatgcggt
     7801 aacagagtct ggcgctcctt tgtgttatgt agcggttgat gaagcacgtc aattactata
     7861 cggggcgaac tatcataaag gtgaagtaaa tgtctaccat atattaggag atggcgaact
     7921 gaaagcagca gattcaatat tccatcaaga agcgacaggt cctcataaaa accaagatca
     7981 tgcgcatgtc cactatacgg atctgactcc tgaccaacga ttagtcgtat gtgatcttgg
     8041 tacagatcgt gtgtatactt atgatgtgtc cgaaaacggc aaattgaatc ttgtaactac
     8101 cttcactgca gagccaggaa caggccctcg ccatcttgtc tttcatccaa atggacaatt
     8161 cgcttattta tttggtgaat tagacagcac agtccgcgtg ctttcttata ataaagaaac
     8221 aggtgctttt gaagaattac agaaaatatc gaccttgcca gaagaattcg acggtgaaaa
     8281 tggaggggca gctatccgca tttcaaatga tggcaagttc ctttatgctt ctaaccgagg
     8341 acataactcg attgctgtat tcgcagtttt tgaagatggt tcccacattc aaaacaagca
     8401 aatcatctca acagaagggg attttccccg cgatttcgca ctagatccaa gcaataattt
     8461 tgtcgtgtgt gccaatcaga acaccgataa tctgacatta tatcgccgtc atcctgaaac
     8521 aggattactg gaattactga aaaaagatgt ctatgtacca gaatgtgtct gtgtttattt
     8581 tgatcgttag taagaaaagg agaatgaaat gaatcagcaa gacgtattag acagactacg
     8641 ggaagaacta aatattcctt tctttgatgg gaaaatcgaa gataaagagt atagcgaaga
     8701 ggaatatcag aaactcaaag ccgatttaca gaactacttt gaacaatatg tacagaatgt
     8761 agaaaactaa tcagctttcc atactatctg aaaagaacat caggacaaga cttttccgtc
     8821 tatcctgatg ttcttttcta caaataaaaa accgccgaga tggttagact cgacggaaaa
     8881 ggagtattta ctcgattttg agtaagtaca aggttgttgt tggttatagg tatgtcatta
     8941 tcataaagga ttttgatcac tctatcataa aggtaatatt acatttatat tagataactc
     9001 tagtttttat tgttatcaaa gatttctttt attttttcaa caacaaatta gtcatttttt
     9061 ggatggcagc tgtcaattgg tctgtcgctt ggtcttgttt tgcttcagat gtattgaata
     9121 gatcttcatc tacccctgat tcaagatcga tactttggat catttcttca cagcctgcta
     9181 catatgaaag ataagctttt tcaaaatttt tatgaatgcc cattgctttt gctggtggac
     9241 gcaaagtact gactttctta agcattgccc gatatttttt tgttccttct tcaaataaca
     9301 ggatgatctc ttcaagagtc gatttgtcca gttcacttgt ttttccttca tcaattgcgt
     9361 ttctgatcac ttcatatttc ggatgcattc cttctccgat tgcttctgtt tccttgacga
     9421 tttgatcgat ggactgcaca taatagccaa tattcggtaa catgttcgtt ttcctacttt
     9481 ctccattgtt gttattattt attctacttg tttttatcct caaatgacaa gtacttgaaa
     9541 aaatttaatg gtttctatac agctcgccat tcttattcgt gctcaaagaa accatctgct
     9601 aaactatatt gtttccctgc gacaatgtca tattggatca attgatattc gtcgtaaatc
     9661 tcttgttgcg agcgattgaa ttgcgcttct ttataccact gtccgttttg atagtacagt
     9721 tctcgttcaa acgccggaag ttcctgttct aatgataaaa gcagctgata aaagccattt
     9781 gttgtctgat tcgtctgttc cattagattc gctgcaaaat aaaacggact ggtgattgca
     9841 tcgtgagtcg tttgcttttc taattctcct ttactgtcga acataagaaa ttgtgtttca
     9901 tgcaatgttt gtttgtcatt ttttgcttgg atcgtatctg aatagatccc tggaagatga
     9961 tccccccaaa aaacaactaa agtccttcta gaaagattct tcaattcttc cgtgaactgc
    10021 ttcagtgccg tgctgctata ggaaatatct tgcagataat tctctaagtc aagtgtccca
    10081 cttccatctg aagtttttgc aggatagtct agattatcat actttccatc gtagggcata
    10141 tgtgtctgca tcgtgactaa atgaatgaac tgaggtgttt tttcttcctt gagaagtgtc
    10201 attacttctt tgtaagctga ctcatcagaa atatatggat tattctctat cgtatccgta
    10261 taggtcatcg tacgctcgct gatgaactgg tcaaatccta atgtctgata aacatcctct
    10321 cttttataca tcgaagtatt gtatggatgg atcgctgtcg tctggtatga ttgtgcgttt
    10381 aacgctgaaa cgatagaagg aagctgatcc atttttggta caagcatcgt gtaaggggtg
    10441 gtgagttgcg cattaaataa cgccatagaa aaaccagtca atgcttcaaa ttcgatattt
    10501 gctgttccac cgccatagtt ttgagaaagc atattgccac tatatgtttg atcagctact
    10561 tcataataat ctgccaatgg ctcaccagat acttctacgc cgtttaatcg actaggatct
    10621 gagaaacttt cactcatcac gaacacgatg ttcggcgact cttcctctac agttttattt
    10681 ttttcatcgg ctagcttctg gtatttttca gtgatttctt tgatcttttc tttcgaatat
    10741 ccttcaggtt catccattgg ttctaccttc agattgtaca aaaacccacc gataaaacca
    10801 gtgttatagt agttcatttt ctggctgtaa ggtatccata gtgccgtttt attatatgct
    10861 tttcgcaaca aattatcggg attgttgaaa ttactgatat accctagcag tccaattgcc
    10921 acaagcagtg tcgttaatcg gatcaattgg aatcgccttc cttttttcag gcttcgaaac
    10981 atgtaccagc aaaacaatcc tatcacggtt cctgcggcag ctaagatcac tgtaaacagc
    11041 atagtgccgg tcatatcttt cagcaatccg atttcagtga tcatcttcaa atcgtctgga
    11101 taaatcggtt cttgccgata actcattttc atataatttg caaaacctaa tacaccgatg
    11161 gctacgacat acgtaaggga acctaaaggg acagatcctg ctagtgcaat cagaaaaata
    11221 agaataatca gcaagacgag acaggctaag aagaattttt ccgtatgcca agaaaaagca
    11281 aatttcaacg caaggtctac ggatagatca ttttgacacc actgcaaaaa taaattacta
    11341 aaaacaacaa gaaaaaagat actgatgatt gctgctgttc cttttaaaat ctttttctgt
    11401 ttggtcgaaa gtctgcttat atatcttttc aaatgcttgc tccttcattt ctatcaataa
    11461 aagaatgttg cttctcttat tttagttgta cgaaattcga aagtaaagaa cggaaagaag
    11521 aattaatgaa atccaaatat tctctttctt tatggccttt ctggtttctt taataagcgg
    11581 acctttcgta ctccagaaat tttttctagc tgttgtttca cttgattgag ctgttcaaaa
    11641 tcagtactgt ctatgtcaat caatatgtag acatgctcat tcttatggtt acgcgatata
    11701 tctgcgatcg ctagctcgtt ctcattcagc agagaaaata tctctgctaa tatattggtt
    11761 ttcttttgat aaaatatagt gaatcgaaac ggcgcttgga aaaacattcg tgcggatgga
    11821 taattgaccg cttctctaac cgtaccgaac aaaagataat cttttaaggc ttcaactgcc
    11881 aaacgtccgc tgtctgccaa agcttcctga gtcgatcccc ctagatgagg caacataagt
    11941 atacggtcat tttctaagaa ctcttcttct ggaaaatcag tcaagtattt agccagatgt
    12001 tgtttttcca atgcttgcat aacagcatat ttatcaacaa tttcacttct tcctacattg
    12061 ataagtacag catctttttt cattttttct atattcgcct gatcgatcaa tccttttgta
    12121 tcttctgtca acggcagcat gactacgatg taatccgagg tagaaagaac tgtttctaaa
    12181 tctgcttgcc gaacataatc caactgggca tcgcggatag aataacccaa gacatccatc
    12241 cctaagctat agcaacttag cgctactttt gtgcctatcg ctccaagacc aagaagaccg
    12301 atggttttgc cttgcaattc tcgtcccaca taagcacttc ttttgttttc tgcctgttct
    12361 aaaatattag gacctgttag tgtttgtacc atacgacttg cttcgattat cggacgggaa
    12421 gataagagca aacagcacaa aacaagctct tttactgcat tagcatttac acctggtgta
    12481 ttcatgacga tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca
    12541 ccagctcttg aaattgctag caaatctggt gtaagccatt catctttcac tttcgtacta
    12601 cgaataacta ttccttcggc tggcaaatca tctattattt tgaaatattc cccttgtaac
    12661 ttttctatct cttgttctgg aatagcatca atcaaatgaa cgtgatacat aaaaccccct
    12721 ccttactaac aagtatctcg aaatttctgc aattagacaa ctgtttacca tttgacttcc
    12781 tagaagcaaa aagagcagtt agagcagagt aggctgagtg ataaaaataa ggctatgaca
    12841 agttactgcc ataagcctta ttttcgagag caaaatattg agggtgagat atcttgataa
    12901 atggtgttcc gttagctgac attgataata agactaaatt tcaaaaaagt tgaaaagcaa
    12961 atttcgtcaa tctcttctta tttcttaaaa ctaagtggct ttttcacaac ctctttttat
    13021 ctaataacca ctgtttccaa ctcaactaat tttgcccacg tttcaatttg atctgttgtt
    13081 agatttagcg agacaacggt atgatgaccg ccacctgcct gaatccatga atggacacct
    13141 tcatggaaat tcggtttagt tttccataac acacgtgcaa caggtaaatt aggtgcttca
    13201 gtcgttggtt caaaggcttc tacttcattg atcagcagtt tataatgcgt accgaaatct
    13261 gccatcgata cgactacccc ttctcctgct ttcccatcaa atacaagacg cgccggatct
    13321 tctctatctc ccattgataa aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc
    13381 ggatctactt ccatcatatg ggattccaag attgcttctt ggccactagc tagctcgtaa
    13441 gtataatctt ccatgaatcc tgtatctttc cctcgagcca tgattttcag caaccgatca
    13501 atcgctgcag ttttccagtc gccttcccca gcaaaaccgt atccttctgc catcaaacgt
    13561 tgtacagcta gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa
    13621 gcgttatagc ctccttcttc taagaaacgg cgaataccga tttcctgctg agcttgtact
    13681 tttacatgtt cctcccatgt cttttcttca taatcaccat agtcgaaggt atacaattcc
    13741 ttgtactctt caaataattg atcgacttct tcgtctgata cgcgatcgat tacttgtaca
    13801 agatctccga taccaaaata atcaacagtc caaccaaatt gaatctgtgc ttccactttg
    13861 tctccttctg tgacacccac gtttctcatg ttgtcaccaa aacgagccac ttttattcca
    13921 aaactttcat tataggctac tgcaacatcc atccaatcag caatctcttt ttggatctca
    13981 ggtcgtttcc agtagccaac gacgatttta ttttgtttat ttaatcgagc attgatgaac
    14041 ccatattcgc ggtcaccatg agcggattga tttaaattca tgaaatccat atcgatcgtt
    14101 ttccaaggaa tgctttcgtt atattgcgta gctaaatgaa gcaatggttt ttgtagaagc
    14161 tttgtcccgc ggatccacat ttttgctggt gagaaagtat gcatccaagt gatcacacct
    14221 gctacttctt ctcgataatt gacctctttc atgatttttg tgatcgtatc agcagtaact
    14281 gctaattctt gaaggcggat tggataaggc aattggccat tttcattcag ttcatcgacc
    14341 atttcttgtg catgtttctt tacttctcgc agtgcttctt ccccatataa atgctgtgat
    14401 cctacaacaa accaaaattc tttttctcca atattcaaca taattagcca ctccttttat
    14461 ttgttttgtc cgtaatacgc attttttccg tgttttcgta aataatgttt atctaaaatc
    14521 cgttgaggca gctcttctga aaatgaattg agctgtctgg taaataaatt catcttgcat
    14581 acttcatcta gtacgactgc gttcattacc gcactttgcg catctttccc ccaagtaaat
    14641 ggaccgtgtc cgtgtaaaag tacccctgga attgctaagg gatcaatttt gcgttctttg
    14701 aatgtttcta tgataacttt tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt
    14761 gtcaaaaatc gagcacaagg taccgatcca taaaaagtat ctgcatgcgt cgtccccatc
    14821 gcaggaacat ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg
    14881 attcctccga tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt atccgaagag
    14941 ggattgagtt taccttcaat gatatgattg tccaagtcca caacgaccat gtcgtctgct
    15001 gtcatctctt cataactgac tccgctaggt ttgataacga aatatcgtgt ttcaggatca
    15061 aaagcgctca catttcccca tgtatatttg acgagtccgt gtttaggcaa gtcaagattt
    15121 gcttggtata cttcttcttt cagttgttcc agcattttct atcactcctt tttcttgcaa
    15181 ttgatgaatc gctgtttttt caatgtcgat cccctcacgg taacgtctca taaatatctg
    15241 atagcctgct atttcttctg ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac
    15301 gtcttctgcc aagaagtttt ctaaagactt cccatgtcga tccatcatat aggctgctaa
    15361 taacgcgatt ccccacgctc caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc
    15421 catagcagat gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc
    15481 cactaaacga tcaatttgga cattctcaga tttcaatata tccattccga ttctcattgc
    15541 accaaaagca ctggctaaat ggatccgcat gaaattcgct aaatcaaaac ggctacctgg
    15601 tgtacgtaca aataatggcc ttccttcatt tactcctgta atgttttcac cggaaaaata
    15661 accataagaa agtaatcccc cgcaatccgt atcgccttct agcgctttgt tgaataatac
    15721 cgtgaataat ttatctctgc ttatctccat acccaaagaa ttggtaaatt cttcaaaaat
    15781 actgacccaa gcattgatat ccgaagagca gttattcgta tgaaccatgc ctactagttc
    15841 tcctgaaggt gtagtgacca tgtcgatttc cggatggaca ttttctagtt ctttttctaa
    15901 tacgatcata gcgaaggcgg aagtaccagc cgaaatgttc cctgtccgtt tggctacact
    15961 gttcgtagcg accataccgg ttcctgcatc cccttctggc ggacaaaccg ggattcctgg
    16021 tctcaaattt cccgtagggt cgatcagata agcccctgat tcgcttaact tgccagcttc
    16081 ttctcctgct actagaactt taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt
    16141 ttcgattaac tgaccaaaaa tatttttcat ttcttgatgg taggttttcg ttgaaggatc
    16201 gataggaaac atgcctgatg catctcctac gcctagtact ttctcaccag taagctgcca
    16261 atggacataa cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt cttctttatt
    16321 caaaatagat tgatacaaat gagcgatact ccatctctgc ggaatattgt attgaaattt
    16381 ttccgttaat attcgttctg cttcttcagt gattgtattt cgccaagtac gaaatggtac
    16441 tagtaactgt tccttgctat caaatgctag ataaccgtgc atcattgcac tgaacccaat
    16501 cgacccgatt gtcgtaagat tctcgccata gatttcgaat acttctgctg ccatttttcg
    16561 gtaactgacg cgcagtcctt tccaaatatc ttctaatgaa taggtccaga taccattttc
    16621 tagtttgttt tcccactcat aactaccagc ggcaatagga gtaaaagatg aatcaatcaa
    16681 tacagctttg atcctagttg atccaaactc aatccctaaa gatgtacgac catctatgac
    16741 atctaacata ctttgcgcat ttacttcctt cataatgatt taactccctc cttctcgtca
    16801 aacctgatca aaccgtcttt ttagtagcgc ttacaatgat tattgtatac ttttgtacgt
    16861 acatgtcaag cggttattta caagaataca ataaaaaaca cttgttttgt acgtacataa
    16921 gttataaaat gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat tctagaagga
    16981 gagaatactt atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc
    17041 atttggcggc attctatttg gatatgatat tggtgttatg accggcgcct tacctttttt
    17101 acagcatgat tggggtttgg caggtaaagc ttctttgata ggatggatca cgtcttcggt
    17161 catgttaggt gcgatccttg gcggctcttt atctggtctt ctttctgata agcttggacg
    17221 acgaaaaatg attcttcttt ctgcacttat ttttatggct ggttctgtcc tttctgcatc
    17281 tgcccctcac aatggttctt attttctgat tgcagcaagg attcttctag gtttagctgt
    17341 tggcgctgca tctgccctag ttcctgctta tatgtctgaa atggctcctg ctagactaag
    17401 gggaagattg tcagggatca atcaagtgat gattgcttct ggcatgctcc tttcctatgt
    17461 tgcagattat ttgctaaaag tgttgcctga aacgatggct tggcgcgtga tgttagggct
    17521 cgctgctgtc cccgcgctga ttttattttt cggtgtttta gctctaccag agtcaccgcg
    17581 ttttcttatg caatctggcc gcttggaaga ggcaaaaaga gtattgaact atattcggac
    17641 accaaaagaa gctgaacagg aattcgaaca aatccagctg aatgtcaaac aagaaaagac
    17701 aaccggaact tcttggcata ctttgttttt agaaaaatat cgttccttag tcattgccgg
    17761 tattggtgta gcagtattcc agcaatttca aggagctaat gctatttttt actatattcc
    17821 gctgatcgta gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt ggccgatcat
    17881 ccaaggaatc attttagttg ctggttcttt gctctttttg gttattgcag acaagttcaa
    17941 tcggcgaact ttattgaaaa taggcggtag cgtgatggga ctttcattca tcctaccagc
    18001 tgttttagga actgtactgg atgctcatac aaattcgttg ttgattctct tgtttttatg
    18061 tatttatgtc gcattttatt cttgcacatg ggctccactg acatgggtaa tcataggcga
    18121 aatattccca ctagctgtcc gcgggcgagc atctggccta gcctcttctt ttaattggat
    18181 tggttctttc ctagttggct tgctttttcc tgtgatgact gcttctatgt ctcaagaaat
    18241 cgttttcggc atttttggga tcatctgctt tttaggggtt ttatttattc aagagatcgt
    18301 accagaaact cgtgggaaat ctttagagga aatcgaacaa agtgcatcta aaaaaactta
    18361 tcctaaacgt atttctatcc atcattctta aagaggttgt gacagaagtg gtcagcttca
    18421 agaaataaga aggaaccgct tagtttccag tcacaaggaa caatttcaaa aaacagcttc
    18481 tcatgtttga tgaatattgt gacttgtgac cagttggtag gagaacaccg tttagtggag
    18541 aggtggtgaa agaaacgttc agccttgagc gatgacaaaa gctctcacaa acattgcgac
    18601 gagtaaccgt aggagcaata agaggcggta aaaacccgtc tcttattttt cgttttcttt
    18661 cactgtttcc cgttcaacta gtacaggttc atagagaata tttggctgtg cctgtccttt
    18721 ttcaatcgtt gtgacgatcc accgtgcggc atcttgtccc aacttttctt ttggatgcgt
    18781 caatgttgtc aattttttat tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat
    18841 cgataggtca tctggaatat gatagccatt ttctgtcaac ttatctagca ttaaactagc
    18901 tatttgatcg ttatagcaaa tgatgccatc taaagcttgt tcttctttca accgttgtac
    18961 tgttttttca gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa
    19021 aaaacgcaca ttatactgtt cacacgcgct gataaaacct ttcatcctgt attttccttg
    19081 aagatcgtcg atttttgtga tgaacaacag acgctgatgt ccttttttta tcaaatattc
    19141 tgctgccata aaaccgactt tcacatcatc taaacaaata taagaggcat ttaactcttc
    19201 ataagcagca ttgatcatca ccatcggaat gccttgttct ctcaaagcaa cataaagtgc
    19261 taagtttggg ttatactgat tacttttcgt aggctcaacg atcaaaccat ctaccccata
    19321 agcaatcatt ttcttcaaac actctttctc caattcgtga tcgttattcg tactggctaa
    19381 aagcaatgaa tacccagatt ctcgtaattc attttcgatt cctcggataa tcgacggaaa
    19441 aatataatca gagagatatg tggtgatgac accgatcgtt tttgttctgt tcaatggtgt
    19501 tttttctttc ttatagctgt catctacata tgtgcccgac cctttttctt tacgcagata
    19561 gccctcattt accaacacag cgattgcttg ccgaatcgta tgtctgctga cgtcatatat
    19621 ttcttgcagc ttgaattctg agggaatggt tgtatttact tcgtattcct cacgaaggat
    19681 cttttgtttt atctcttctg caatatcttg atattttgcc atttgccttg ccccttttta
    19741 tttgtacgta catatatcat aacgagtctg caaggtttat tcaagcaaaa atcaaggttt
    19801 cttgtgctca taaggcatac ggaacaggtt caccaaattc tggaaatcct tgctcattcc
    19861 acgtaaagca ttgcgcgttc gcgtgccgat taggattgtc taacggatcc cctttttggt
    19921 tcttctctgg tcgagcatga tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa
    19981 acgagttgtg accaggacca aattgcttat ttttttcaga agaaacgaat actggctccg
    20041 ataatttatg ccatgattct tttaataaaa gattcttgtg ctcatcagcc cacagcaggc
    20101 ccatggcata gttttcatct gttgcactac cagaatacgt aatgaatatt ttcccattgc
    20161 gagtaaggac tgctggacct tcatttactg aaaaaccccg tttttcccat tcatactcag
    20221 gtatagaaag gaggatctgc ttccccttta acgtccaagg attatccatt tcggatagat
    20281 aaagattaga attcccagta atttcaggat cttgttgggc ccacacataa tatagctttc
    20341 cttgagaaga gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc
    20401 ccttttctat ccaatctgta ttcattggat ctatatgctg attttcaagt acgaacatgc
    20461 ggtgatgatg agattcatca cgtatagttt tatgattgct tgcagcaaag taaatatacc
    20521 attttccatt gatataatgg acttcaggtg cccagatcaa ttcactcatc ggtccttctt
    20581 catgagcctg ccatacaatc aggcttgcag catgttctaa ttcattcagt gactttgccc
    20641 ttcttaattc aatcacttgg tagcctggaa ccgatcctgt aaaataataa taaccatctg
    20701 tatgtttata tacccaagga tctgcccgtt gccgaacgat aggattatta taaagtgtct
    20761 tcatcataac aaatttcctc acattttctt tattctaatt acattccaag agtatttttc
    20821 tatccctaca tttaattcgt catgtttttc tattgaaaaa tagtttattt caggttttat
    20881 tttttctggt tcttcacgtg tatttttgtc caacagatcg ctactggtta atgtgtagtg
    20941 ctctccttga tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt
    21001 gcgattgatc aagaaaatag tcagctgatt gccgttatct acgatcacac tgtccacata
    21061 aggaacattt tttatctttt cacaatcata aacagggcaa atgatttgtt gtttcaatac
    21121 ttgaccttta gcatatttcg ataagtgttg catcacataa taagttggat tcctccatgc
    21181 ttgtccttta gcatctgtca gtattaaagg tattgtgttt actaacaatg attgacaact
    21241 aatctttacg cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa ataataatgt
    21301 atcttccata gtaaagtaac accaatcgat gggactaccg acttcaaaat ccctatgtct
    21361 tttttccggt tgggcatgta cattccattc atcaaacgct agatacattg ttttctcact
    21421 tctcaaatat ccttttacat aatcacaagt agcaacgatt tcttcaattt gacgatcgaa
    21481 tcgataagct tttgcaaggt atgtcgctgt atcatcacgc tcatttttag gaggtttagt
    21541 caagtcttct tcatcatact tatcaatata gttatgcaaa gctaaataat ctacattctc
    21601 ataagcttct tctaatgcga tacgatccca ttcagggtac gatggtaaac gtggcgtaga
    21661 gcttccaact aagattgttt caattgaatc atccactaac ttcatgactt ttgaggcttc
    21721 atttgctaac ctaccatatt cataagcact cttagccccc acttgccatg gaccgtctag
    21781 ctcattccct aaacaccata atttaacgcc aaaaggcttt ttttgcccat tttttattct
    21841 taaatcactc cagaaggttc cttttggaaa attacaatat tctactaaat tttttgcttc
    21901 atcaattcct tttgttccta aattcaccgt gaatatcggt ttcgcttgta catgactgat
    21961 ccatttaaaa aattcgtgga gtccaaaaga atttggttct atacctcgcc atgctaaatc
    22021 aatttttttt ggtcgttgtt ctttcctacc tacagtatcc acccaatcat aaccagatgt
    22081 aaagtttcct ccaggatagc gaatgacacc taattctaat tcctttacat aagagaggac
    22141 atcttgtcta aacccgtcac catcagctgt ttcatggccc ggttcataaa tccctgtata
    22201 gacgacactt cccatatgct caacaaatga gccaaacagc cgagaactga tctctcctac
    22261 cgtaaaatcc tctctgattt ttactttcac ttcttcgtcc aacagctttc cctacctttc
    22321 atttcctata gtcaagataa tagatacaag tttccatacg ccaccagcaa agataaaaat
    22381 aaaaggaggg aaaagaaata tgagcagtac aaatcctaat aacaatacgc aacaaagaat
    22441 atttctcaaa aaatcaaaaa atactttcca tgcactttgt tcaaaaattt gtaaaaatgt
    22501 gatatctttg ttttcagaaa tcacatatat ccaggataaa agaaacatgg aataaataag
    22561 taaaactaca tatccactaa ttgaaagtac gattgtctgt tcttctaata attgattaac
    22621 aagaaaataa ataatgatcg ttaacagact aaggaacaag ttcttaccaa aatgttgctt
    22681 tattccttga aagaaaaaat aaatgatatt tcctgatccc tcacctttcc attgcgccat
    22741 tacataaaaa acagcacatg tagcaggcac gatagtaata acaggcaaac acacgattac
    22801 ccagagaata cctaataaga tgtaatcaga aaccacaatc aactgtccat aaaatttagt
    22861 ctcaactatt ttcatcttcc tcactctttc attccagaca tagctgttcc ttgaacaaaa
    22921 tatttctgtg caaaaatata aaccactagt aatggcagca ttgaaataac cgttccagcc
    22981 attacaggtc cataaaaaga aatgttctgc cctgtaaata aagctaaccc tgctggtaaa
    23041 gttctcattt ttgaactact tgtcaatatc aatggataaa gcaagtcatt ccaactattc
    23101 atcagcgtga aaatccctaa tgataaaaga gtaggtttgc tcaatggcaa catgattttc
    23161 aaaaaaatat taaactcact taatccatca atgcgtgctg cctcttccaa agaatctggt
    23221 aaagtaataa aaaaggaacg catcatgaag ataccaaatg cagttgtcat acgaggaatg
    23281 atcaaacctg cataagtatc aagcatgcca agcaaattga cttcaataaa tagaggaatc
    23341 atgaagactt ggaatgggat catcattgtc agcaaaacga aatagaacaa aactgatttt
    23401 cctttgaaac gcatacgagc aaatgcatac cccgcaagag aatcaaagaa tacagaagtg
    23461 atcactgtcc ctccagcaaa aatcactgta tttttgatgt aatctaataa agggatcgtt
    23521 gtccaaacct taatatagtt atctagggta tagctatctg caaatattct aggcggaaat
    23581 gagataatgt ctttttcgtg tttaaaagaa gaaataatca gccacaaaaa aggaaacgca
    23641 ataaccaaca atataactag catactaatc gttcgaatga tttttttacc actaactgct
    23701 tttcgactat tcacagtaac tgtaatcgag ctttctggtg ccatacaggt ttccctccta
    23761 tttcatttgt ttttctttcc gtgacatgta cacattagtg gagatagcta tagttgcaac
    23821 aatgataaac aaaaccgaag acagtgctga tgcatagccc aattcataag gtgctgtaaa
    23881 tcctcgactg taaatatatt gaacagctgt ttctgtctta aattgtgggc caccagcggt
    23941 tgcaacatat acttggtcaa acacttgcat cgaaccaatc agattcgtta aaatacaaaa
    24001 acctaaggag ggaacgatct gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc
    24061 accatccatt tgagcagatt catacaaaga gggagagatt ccctgaatag cagtcaagag
    24121 aatcgtcata gtaacaccaa aattcttcca aacagtcatg atggctaccg tcggcatagc
    24181 aagggttgga tcgcgaaaaa attgagggat cggcaagccg acttgtgaaa acaagtaagg
    24241 aatcgcccct acattcggat caagcaacat agaaaaaata atcgcaattg acgtcaatga
    24301 acaaataact ggaatgtaaa atgtcgaacg acaaaaacga ttgaatctgg tatttttaga
    24361 taatgctact gctgctaata aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa
    24421 atataaagaa tgaatcaacg aattaactgc tcgttcatca ctaaatatac gtgtgaaatt
    24481 ttcaaaactt acaaaatttc ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc
    24541 aaatgttcct aaaagcggta tgacagtaaa taataataaa actaaaaaag atggagttaa
    24601 aaacaaatac gcagcacgat caggacgatt ccaatatttg gtgaatccgt tcatatagtc
    24661 cacctcatct ttcattttga taaaatatac aatcttggtt taaacgaatc tgaataagat
    24721 atccgaacaa tcgctgtgat ttttgcaata acacctatct gcaaatacta ggtcgatcat
    24781 tcagatatct gtatcttgcc aacagtctat cctgtataaa gttctgtact atttttgcag
    24841 taattgattg atggcatcat ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa
    24901 taatttttca atcagtggat tgatgatatc gttattgatc gcagagattt gggtaaaacc
    24961 tggtaaatat ggctctgcat agtccatttg tttcgataat tcagatacaa taggatcatt
    25021 ttttacttct tcatcttttg ctactgaatc caagtaggca ggaaaaccat tttcgatact
    25081 ccatttttta ccaatttcag ttgtattcca atatttcaaa aactcataga cagcttcttt
    25141 tttattatta tctgtacttg ccggaatggc atatcctacg ccatctaaaa tagctgtttt
    25201 tttgtctgaa tcactccctt gtggtaaggt agtcacatta taatcaatct cgtttgcttt
    25261 caatccgtta tttaaccatg gcccattgat ttcaatggct agttgaccag cattcattaa
    25321 attgtccatt tcggcgccag aaatcgattc tggaccagtc ttgtctacat ggatcatttt
    25381 ttggatcgct tttaacgttt taaggttctc aggagacgca aattcagact tagtataatc
    25441 atcgttcact aatttaccgc catttgctaa catccagtta tataacggtg cagttccatc
    25501 cttattgaag acaaaacctg atacattttt actactatca gttaattttg gtgccatttc
    25561 tgtcaattct tcccaagtag taggtggttg ttcagtatct aatccagctt gtttaaacaa
    25621 atcattattc caatacatat acatgccttg tacctgcatg gggacaaaat actgtttacc
    25681 ttctacttca cctaattcaa tagccgtttc tttaaaatca gatttatcta ctccatcata
    25741 ttcccagaaa tcgtctaatg gttgtaccgc tccgttttct acatactgtg caaaatctgg
    25801 ataatttaat gcaataaagt caggtgcttt tttagaagaa atcgctgtag gaagtttttc
    25861 attcaaattg gcccaagtca tgatatccat ttcaattttg atgtttttat catttgtttt
    25921 attaaattca ttaacgattt cttgcaatat ttcaccatca gatgcagtaa acccattcca
    25981 aaaagtcaaa gtcacttctt tatcagaatc agattctgct gaacttccgc caccgcaagc
    26041 tcctaaaata cctgttgcag ctaccaacaa agagccaatt aaccattttt tacctttcat
    26101 tttttgttcc tccttcatct gataaacgac atctgaccac attccaagat agcggtttca
    26161 ataaacaatt gattttccca tcttcagctt ttacatttgt tgaatgactt ggttttactt
    26221 gttcactttt aggaagatta gttgcttttg tatcaaatcc actcatttca gaaaaatcaa
    26281 tcactccctc taatgataat tcagacaagt gaacagttag attcatttct tcttctccac
    26341 ggttaactgc aaatatgaca agttctttgt catttattac ggctatactt tctaaatacg
    26401 gcacttcttc aaaatcttcc gtcgcatagt ttgctgaacg aatttgtggg cttaacacct
    26461 ctccttgtcc atacacagca gcttgcataa atggataata gatcgtctgt ctccaagctt
    26521 ctccgtttgt ttctgtcctg attggtgcaa taacgtttac caattgagca agacaagcaa
    26581 tctttactct atctgcatgt ttcaataaag tgattaatag acagcctaat agtaatgcat
    26641 cttcaaaatt atagttatct tctagtaaag gaggagcaat ctgccaaggc gctgcttgct
    26701 catcttgttc cgctgaatga taccaaatat tccattcatc aaaggacagg ttgatctgct
    26761 tcttactatg tttcttcgcc ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc
    26821 gatccatgtc taaggaacgt gcaagataat gagaaagatc atttttttgg ttgccataat
    26881 attgatgcag agacaaataa tctatttgat cgtacgcttg atcaagaact gttaactccc
    26941 aatcaccaaa tgtaggcatt tgccgattcg aacttccgca taaaaccacc tcaattgaat
    27001 catctacgag tttcatcgcc ttagccgttt cctctgctaa ccgtccatat tcttccgctg
    27061 ttttatgtcc aatctgccaa ggaccgtcca tttcattccc taaacaccat gtcttaattc
    27121 caaaagggct ctcttgacca ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa
    27181 aattacaata ttcgactaaa ttacgagctg catctactcc acgagtaccc agattgacag
    27241 ccatattgat ttcggccccc acatgctgac accagttata aaattcatga atcccaactt
    27301 cattcgtttc aattgtccgc caagctaaat ccaaccttct aggtctattt gctatgggcc
    27361 caatactgtc ttcccaacga aatcctgaca caaaatttcc accaggatat ctaatcatgg
    27421 gaatgttcaa ttcttttacc aagtgaatga cgtcttggcg aaatccactt ttattagctg
    27481 atggatgatc tggctgataa atcccttcat atactgctcg tcccatatgt tcaataaatg
    27541 aactgtacaa ccgcggatca actttgccta cacgcttgac actactaatc aacattgtgg
    27601 cttccataac tgacctcctt taatttatac atgaataata cacctcgttg aaagcgttgt
    27661 caataatata aatgttaaac ttacttattt tttatcgatt ttaattaacc aaaatattaa
    27721 taatgattaa aacaaataat ttaatataac ttttattaac aaatatgtta atatataaga
    27781 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
    27841 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
    27901 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
    27961 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
    28021 tcgagccttc gtgtcgataa aatcgatatt agttttccag aaaaaatatt caatgctttt
    28081 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
    28141 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
    28201 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
    28261 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
    28321 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
    28381 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
    28441 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
    28501 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
    28561 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
    28621 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
    28681 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
    28741 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
    28801 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
    28861 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
    28921 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
    28981 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
    29041 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
    29101 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
    29161 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
    29221 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
    29281 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
    29341 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
    29401 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
    29461 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
    29521 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
    29581 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
    29641 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
    29701 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
    29761 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
    29821 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
    29881 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
    29941 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
    30001 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
    30061 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
    30121 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
    30181 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
    30241 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
    30301 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
    30361 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
    30421 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
    30481 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
    30541 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
    30601 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
    30661 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
    30721 ccttccactt ggatcaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
    30781 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
    30841 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
    30901 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
    30961 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
    31021 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
    31081 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
    31141 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
    31201 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
    31261 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
    31321 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
    31381 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
    31441 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
    31501 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
    31561 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
    31621 aaaatggcga gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag
    31681 caggttacca taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt
    31741 tctatcacta caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc
    31801 tgtttcactc ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc
    31861 gcatagtcga tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt
    31921 ttatcatatg tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa
    31981 ttaatagccg tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt
    32041 aaattactat tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg
    32101 atttagatcc aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa
    32161 ttgaaccttt aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttca
    32221 tgagaaaaaa atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt
    32281 tgtcaggagg aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca
    32341 gtccgagaac cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt
    32401 cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt
    32461 atcgaacaat ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg
    32521 accaccagaa agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg
    32581 ataccctctt gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga
    32641 gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct
    32701 atggacaaga gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat
    32761 ggatgaatat ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat
    32821 ttgatctcac tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc
    32881 aagctcataa gaagtggtta tcttacagct ggcctgaata tcagccaaaa gcgcataaag
    32941 gactaggaat gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg
    33001 gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat
    33061 gatccaacaa aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta
    33121 gatttttatg ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt
    33181 taaatatatt catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt
    33241 aagaaacaat tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa
    33301 accaagaagc tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg
    33361 cagatagctt tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac
    33421 tccaagctct ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccca
    33481 aaaaacaagt gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt
    33541 tgagttataa cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg
    33601 ctgcttcaaa taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct
    33661 tgcctgtttt cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa
    33721 taaatcatct agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc
    33781 aaattcttca agataccttc gctctttttc tttctgatag cccgtcggta aaagtgtcac
    33841 tttgtaatca taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg
    33901 atgattggta taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct
    33961 ggcatttgca ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt
    34021 ttcctcatct acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc
    34081 tgtgagcttt gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga
    34141 ttttcttgta aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct
    34201 tcatgcgaaa aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt
    34261 tcattcattg gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt
    34321 aatacggatc ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt
    34381 atctctacat aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa
    34441 acgcgtctca tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat
    34501 taccaaagtc tcttatttta aattgaatac attataatga tactgtaaat atatctttat
//
LOCUS       DAZKPO010000024        34118 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000024 DAZKPO010000000
VERSION     DAZKPO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.024"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8NZ_001107"
     CDS             complement(99..1229)
                     /locus_tag="ACG8NZ_001107"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0538801.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8NZ_001108"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8NZ_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0538802.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8NZ_001109"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8NZ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0538803.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8NZ_001110"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8NZ_001110"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0538804.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8NZ_001111"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8NZ_001111"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538805.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8NZ_001112"
     CDS             4880..5692
                     /locus_tag="ACG8NZ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538806.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8NZ_001113"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8NZ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538807.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG8NZ_001114"
     CDS             complement(6502..6732)
                     /locus_tag="ACG8NZ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538808.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG8NZ_001115"
     CDS             complement(6716..6913)
                     /locus_tag="ACG8NZ_001115"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0538809.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACG8NZ_001116"
     CDS             complement(7059..7937)
                     /locus_tag="ACG8NZ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0538810.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACG8NZ_001117"
     CDS             complement(7976..9094)
                     /locus_tag="ACG8NZ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0538811.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACG8NZ_001118"
     CDS             complement(9091..11190)
                     /locus_tag="ACG8NZ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0538812.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACG8NZ_001119"
     CDS             complement(11241..12062)
                     /locus_tag="ACG8NZ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0538813.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACG8NZ_001120"
     CDS             complement(12059..12868)
                     /locus_tag="ACG8NZ_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0538814.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACG8NZ_001121"
     CDS             complement(12889..13383)
                     /locus_tag="ACG8NZ_001121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0538815.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACG8NZ_001122"
     CDS             complement(13395..13895)
                     /locus_tag="ACG8NZ_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0538816.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACG8NZ_001123"
     CDS             complement(13960..16656)
                     /locus_tag="ACG8NZ_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0538817.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="ACG8NZ_001124"
     CDS             complement(17084..18157)
                     /locus_tag="ACG8NZ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0538818.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACG8NZ_001125"
     CDS             complement(18154..18981)
                     /locus_tag="ACG8NZ_001125"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538819.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACG8NZ_001126"
     CDS             complement(18982..19788)
                     /locus_tag="ACG8NZ_001126"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538820.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACG8NZ_001127"
     CDS             complement(19800..20885)
                     /locus_tag="ACG8NZ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538821.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACG8NZ_001128"
     CDS             complement(20902..21444)
                     /locus_tag="ACG8NZ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0538822.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="ACG8NZ_001129"
     CDS             21702..22691
                     /locus_tag="ACG8NZ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0538823.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="ACG8NZ_001130"
     CDS             22688..23425
                     /locus_tag="ACG8NZ_001130"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0538824.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="ACG8NZ_001131"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="ACG8NZ_001131"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0538825.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="ACG8NZ_001132"
     CDS             23989..24621
                     /locus_tag="ACG8NZ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0538826.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="ACG8NZ_001133"
     CDS             24706..25353
                     /locus_tag="ACG8NZ_001133"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0538827.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="ACG8NZ_001134"
     CDS             25358..26341
                     /locus_tag="ACG8NZ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0538828.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="ACG8NZ_001135"
     CDS             complement(26744..28258)
                     /locus_tag="ACG8NZ_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0538829.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="ACG8NZ_001136"
     CDS             28396..29202
                     /locus_tag="ACG8NZ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538830.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="ACG8NZ_001137"
     CDS             complement(29377..29946)
                     /locus_tag="ACG8NZ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0538831.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="ACG8NZ_001138"
     CDS             complement(30029..32089)
                     /locus_tag="ACG8NZ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0538832.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="ACG8NZ_001139"
     CDS             complement(32366..33844)
                     /locus_tag="ACG8NZ_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538833.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT        10255 a         7082 c         5747 g        11034 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctctttta
//
LOCUS       DAZKPO010000025        32713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000025 DAZKPO010000000
VERSION     DAZKPO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.025"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            309..629
                     /locus_tag="ACG8NZ_001140"
     CDS             309..629
                     /locus_tag="ACG8NZ_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0538834.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            783..1688
                     /locus_tag="ACG8NZ_001141"
     CDS             783..1688
                     /locus_tag="ACG8NZ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538835.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1685..2458
                     /locus_tag="ACG8NZ_001142"
     CDS             1685..2458
                     /locus_tag="ACG8NZ_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0538836.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2639..4063)
                     /locus_tag="ACG8NZ_001143"
     CDS             complement(2639..4063)
                     /locus_tag="ACG8NZ_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0538837.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4274..4720
                     /gene="nrdI"
                     /locus_tag="ACG8NZ_001144"
     CDS             4274..4720
                     /gene="nrdI"
                     /locus_tag="ACG8NZ_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0538838.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4785..6767)
                     /locus_tag="ACG8NZ_001145"
     CDS             complement(4785..6767)
                     /locus_tag="ACG8NZ_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0538839.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6781..7677)
                     /gene="murQ"
                     /locus_tag="ACG8NZ_001146"
     CDS             complement(6781..7677)
                     /gene="murQ"
                     /locus_tag="ACG8NZ_001146"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0538840.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7680..8741)
                     /locus_tag="ACG8NZ_001147"
     CDS             complement(7680..8741)
                     /locus_tag="ACG8NZ_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0538841.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8952..9479)
                     /locus_tag="ACG8NZ_001148"
     CDS             complement(8952..9479)
                     /locus_tag="ACG8NZ_001148"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0538842.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9546..11048)
                     /gene="eat(A)"
                     /locus_tag="ACG8NZ_001149"
     CDS             complement(9546..11048)
                     /gene="eat(A)"
                     /locus_tag="ACG8NZ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0538843.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11306..12427)
                     /locus_tag="ACG8NZ_001150"
     CDS             complement(11306..12427)
                     /locus_tag="ACG8NZ_001150"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0538844.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12553..13494
                     /locus_tag="ACG8NZ_001151"
     CDS             12553..13494
                     /locus_tag="ACG8NZ_001151"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0538845.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13644..14540)
                     /locus_tag="ACG8NZ_001152"
     CDS             complement(13644..14540)
                     /locus_tag="ACG8NZ_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0538846.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14809..16149
                     /locus_tag="ACG8NZ_001153"
     CDS             14809..16149
                     /locus_tag="ACG8NZ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0538847.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16367..16810
                     /locus_tag="ACG8NZ_001154"
     CDS             16367..16810
                     /locus_tag="ACG8NZ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0538848.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17314..18732)
                     /gene="nhaC"
                     /locus_tag="ACG8NZ_001155"
     CDS             complement(17314..18732)
                     /gene="nhaC"
                     /locus_tag="ACG8NZ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0538849.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19135..20358)
                     /locus_tag="ACG8NZ_001156"
     CDS             complement(19135..20358)
                     /locus_tag="ACG8NZ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0538850.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20389..21228)
                     /locus_tag="ACG8NZ_001157"
     CDS             complement(20389..21228)
                     /locus_tag="ACG8NZ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0538851.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21245..21925)
                     /locus_tag="ACG8NZ_001158"
     CDS             complement(21245..21925)
                     /locus_tag="ACG8NZ_001158"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0538852.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21927..23000)
                     /locus_tag="ACG8NZ_001159"
     CDS             complement(21927..23000)
                     /locus_tag="ACG8NZ_001159"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538853.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23056..23294)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23337..23570)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23647..24318)
                     /locus_tag="ACG8NZ_001160"
     CDS             complement(23647..24318)
                     /locus_tag="ACG8NZ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0538854.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24405..27053)
                     /gene="mgtA"
                     /locus_tag="ACG8NZ_001161"
     CDS             complement(24405..27053)
                     /gene="mgtA"
                     /locus_tag="ACG8NZ_001161"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0538855.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27395..27569)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27742..28179
                     /gene="ndk"
                     /locus_tag="ACG8NZ_001162"
     CDS             27742..28179
                     /gene="ndk"
                     /locus_tag="ACG8NZ_001162"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0538856.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28201..29067)
                     /locus_tag="ACG8NZ_001163"
     CDS             complement(28201..29067)
                     /locus_tag="ACG8NZ_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0538857.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29227..31320)
                     /locus_tag="ACG8NZ_001164"
     CDS             complement(29227..31320)
                     /locus_tag="ACG8NZ_001164"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0538858.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32069..32692)
                     /locus_tag="ACG8NZ_001165"
     CDS             complement(32069..32692)
                     /locus_tag="ACG8NZ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538859.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         9459 a         6832 c         5788 g        10634 t
ORIGIN      
        1 ttgcccgata atatatagaa ataaggtcta gttactgttt tttttaaatg attaataaat
       61 aacaacttct ttctatgaaa ttgcaaagca ttcatcttct tttccgctaa cgatatatcc
      121 gtcagtgctt ggtacacttt actaaaaagt atgagattgc cgatatctgg ataggtgtgc
      181 tatactacag aaaaagatat acaatctgcc accgggtagg ataaagagga tgatcattcc
      241 attaggtctt tgaagggatg attttcctct ttttgtgtat ttttttatag ttaaggagga
      301 atgtaagtat ggcatggatg gaattaatca tagcaggaat tttggaagta ttctggtcaa
      361 cgatgatgaa atggagtgat ggtttttcta aaatcaatta ttctttttac acagtgattg
      421 gcatgattgc tagtttttac tttttgtcaa aggcaatcaa aagtttgccg atgagtctct
      481 cttatcctat ctggacaggg attggcgcag ttggttcggt cataattggt gtggtcttgt
      541 ttcatgataa gctgaatctt tccacttggt tctttgtggg gatgctactc atcagtatca
      601 ttggcatcaa gattactagc ggccactaga cacttggaat aagtactgat cagaaaaaat
      661 aagccttttt atttaatatt caattttttt atgaaaaaat aagaaataag tccaatgacc
      721 tagcgacttc tccactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat
      781 ttttgaaaag tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca
      841 gcgatgtcag ttttagctta tcccctggca aaatcgttgg attagtaggt cctaatggcg
      901 ctggaaaaac aacgatcatg aaagctattt taggactgat ccattactca gatggaacaa
      961 tctgtattgg tgaaaatgag atttctccct ctagtcataa agggttggaa caagtaggag
     1021 cattgatcga atacccgggt atctatccat tcttaacagg ctatgatcat ctgaagttat
     1081 tctctgaaac caacgatgta tctgctatcg acactatcgt gaatcagctc aagatggaaa
     1141 agtacattca caaaaaagcg aaatcctact cgttgggaat gaaacaaaaa ttgggaattg
     1201 ctcttgcctt actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg
     1261 atccacaagc aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct
     1321 ctttcttgat ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc
     1381 tgatcaatca aggaaagatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc
     1441 aagatttcat cgttatctct acaacaaatg atctagctgc tcgaacgatc ttgacagaag
     1501 ctggatatac tttggaagat cctgataaaa tcaagctttt aaaagaaaac gaagaattat
     1561 taggaaccgt gatccagcta ttgtctgcac aaaatatcca tgtgacagac gtccagcatc
     1621 tgacgaatga tttggaatct tctctattgg aaattctgga ttcagtaaag ggggaagaag
     1681 aaaaatgaca ttgcttgtaa aacaagaatt atttaaattg atcaagaaaa agtcaactgc
     1741 tgttttatct gttctattag ttgttttgct cataggaaca gcacttctag ccaaaaaata
     1801 cacgacgatc attgatccag ttgaaatgac tgctcagctt tttagtgcca catcttggat
     1861 tgtctttatc atgattgcag ctgcaagcac gatcatttcg atggaagcac aatatggtac
     1921 attgaaaaat ctcctttatc gaaagtattc tcgaggagaa atattagtaa gcaaatggat
     1981 cacattagtt atttattctg tttatttata ccttttagct attattgtca ctgtgttgat
     2041 gaaactgatt cttttccctt cgatttcctt cacggagaag gtttctactg gtcaaacact
     2101 gatccaaagc ttgttcactt atactttggg tagttatatc ggtttatggc tgattttaag
     2161 tttagtgttg atgattgctt gctttatcaa tagttccggc gcatccatct cagcaggaat
     2221 cgtcttttat tttgcttcgt caatcatatc tggtatcctt attgcactca tccaaaaatg
     2281 ggagtggatc aaatggaatc cgatcagtat gttgaatctg caaaaccaaa ttggaaacga
     2341 agagatcatg aagcaattga ctcatctttc tactaatcaa atgctattcg gtaatttagc
     2401 ttacatcgtt ttgttcttag cattaggata tctcgtattt aagcgaaaaa atgtatgatg
     2461 actcatcaaa aaagaggctg tgaaagagac gttcagcctt attgccgatt ggctgtctct
     2521 taaacaccgt ttattctgaa aaaggagctg tgacaacatt tgtgtcacag ctcctttcct
     2581 agtatgtttt tgaataaacg acaggctcac aacctctctt tatacttctt ttatacttct
     2641 atacgtgaag catcccagtg gcaaataaaa ccattgcaat cactccgacc atcgtcacac
     2701 ctataagtaa aatcttatct aagtggatat gttgtacagg ctcgttttgc tccttacgtg
     2761 ctttgaggta aaaataaatg cctggcaaga aaagaacaag aacaagcatt acttcattaa
     2821 aggctacgac tagcatagcc catacttgaa aggcacttgc taaaagccct actaaaaatt
     2881 ggacccattc tttccgctga taagaaagtt tcatttggta caatcctaag aaaagccacg
     2941 tgaagaaaat agctgctgta ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata
     3001 ataatgaaag taaaaagaca ttcgtacata acgtcgtcaa tacgatagca taagttggtg
     3061 ccccattttt gttgagtttt ccccatgctt taggcaaaag tccgtcttca gccatgtttc
     3121 ccattgtttc aatcggtaag atcgtccatg agagccaaac accaactaaa gaaatgatca
     3181 aaccgccgtt gatcacgact gctccccatt taccaaccat catttcaaac acatagccca
     3241 tagcaggctg cgccttgatc gtttgcagct gattcatcgt cattgtccca tatggaagca
     3301 tggaaaccaa gacgtaaata ataatcaagc cgaatatccc tagaatcgtt gctttcccaa
     3361 catctgattg tttttttgct cgtgaaccta cgacagaggc tccttcaata ccaacaaata
     3421 cccaaagcat caccatgatg caactcaata cttgactgga aaacgggatc ggctgtccat
     3481 tcgcattcaa tataatgtcg ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc
     3541 tgatagctac tacaataaat aaaaataatg gcactaattt acagaatgtg acgatagcat
     3601 tgacgaaact agcattttcg actccccgat tcactagcca tgcatatccc cataaaaaga
     3661 ctgtactcat cacgatcgat gccagatttt gcccaccttc aaatactggg aagaaatatc
     3721 ctaatgcgct catcaacaat gttgcaaaag ctacgttgcc taaccagcat gtgagccagt
     3781 aaccccaacc gctgataaaa ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga
     3841 tcccgccttt taaatctgga cgttttttat tgatattatt gatcgacaag gagagcatca
     3901 acattgctgt tcctacgagt acccaactga aaatagctgg gccgccagcc gatccggaga
     3961 tgtccgtcat aataccaaat acgccacctc caatcgctga accaattact aaggcagcaa
     4021 gtggaattaa accaattcct tttttctgtt gctctgtatc catgtaagca tcctccaaaa
     4081 taaaaccgct tttatcaaaa agtataacaa acctttttta tttttactta attaataaaa
     4141 actttaatag aaatataatt ctaccggctt ttttctcatt atacaagcaa agcgcctagt
     4201 ccctttccaa attacaccac caaaaaactt aaaaaagatg ctatactaaa aggaaagaat
     4261 tggaggatga tccatgaaca ttttatatat atcgatttca ggaaatacgc gtgcctttgc
     4321 caaacatcta gcagaatatg ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt
     4381 tactttaaaa gaaatccatg aaaatagtga ttttacgaaa gaaacagaac cattttttac
     4441 tttcgttcct acttatttag acggcggaaa tggtttagat aacggtgata cagaaatctt
     4501 gaccgaaacg atgcgtgaat atctggaaca tgaagacaat cataaacttt gtcttggtgt
     4561 agtcggtagc ggaaataaaa acttcaataa tcagtattgt ctaacagcca aacaatatgc
     4621 acaaacattt ggctttcctt tcttggcaga ttatgaatta cggggaacac catctgatgt
     4681 gacacgaatt tatcagattt tagcagaaaa caataaataa taaaaaagaa gaagctgaaa
     4741 aatagatttc agtttcttct ttcgtttcta ttgtttctat catattaagc tttgacatcc
     4801 atcacaggtg cttttgcttt tgctttacct gttttttcca agacaaagtt ttttacacga
     4861 tccatattgg ttaccaagac gatcatggat gtttctttgc ctgctgcttt gatcgcttct
     4921 aagtcagctt ctgctacaag cgtatcaggt gtcacttgct gcccttctgt tactttcaca
     4981 tcaaatggcg taccgcctaa gtcaactgtg ttgattccca tatgaagcaa gatttctaaa
     5041 ccatttggcg ttgtgatccc aatagcgtgt ttcgttggga agacagagct gactacccca
     5101 gtaactggag aatagatatg tccgttttct ggttctacaa agtagccttc acccatcatc
     5161 ttttgtgcaa aaactggatc acttgcttta tctaaagaaa caactgttcc gtcagcaact
     5221 gatgagagga tagtcacact ttcagctgaa gtttgaggta ctactttttc aggcacttca
     5281 tttactgtgg ctgtttcagt cgctaattct tctttttcta aacggtcttt aggaataccg
     5341 aagaagaatg tagcaacgaa gccaccagca taagctgcca ataatccaag gatgtagcct
     5401 aaccatttac cgtcagagat caatgggatc aaagcggctc ctgaaggtcc aatagcgata
     5461 gccccgacat ttccgatcat tccgatcact gccccgccga tacctccacc gatacaagca
     5521 gtaataaatg ggcgtcctag aggtaatgtt actccgtaaa tcaatggttc gccgattcct
     5581 aggatcccga ctggtaaagc tcctttgatc atttctgtta attctttatc ttttttgcag
     5641 cgaatccata atgccaatgc tgcacccact tgtcctgcac cagccatcgc aagaattgga
     5701 agcaatagcg tacgaccagt ttcatcaatc atttgcacat ggattggagt caaaatttga
     5761 tgcagtccga acataaccat cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct
     5821 ccaccaacgc caagtaccca gttgatccca ccgaccatgc catcagaaat gaaacctgct
     5881 aaaggcatga tgaagaaaat ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta
     5941 acgatgatat ctaccgcatc aggtactact ttatgcagtt ttttctcaac gattgacaat
     6001 agccaaacag cgaagatgac acctaggatc ccaccttgtc cagctgcaag agcttcacca
     6061 gtaaataagt ttttcagtgg tgcttccgga ttcatccctg tcagcatcac aacggccccg
     6121 ataactccac caagagttgg tgttgcacca aaaacttgtg cagcattgat acctgtatag
     6181 ataactaggt agctaaacat cccgtttttc aagatgttca tcacatcgat atattgttgc
     6241 catgttgctg catcaagtgt tccggctgtg actaagtttg ctaaaactgc ggcaatccct
     6301 gccacgatcc cagtaccgac aaatgcagga atcattggca caaagatatt agaaatgtct
     6361 tttaataccg atttgatttt agatggtttt tgtttcgatt tttgtgccgc tttcatttct
     6421 tgtgcccgtt cgtttacacg gtcttttcca gatgctgatg aatttgctgt agctgtactt
     6481 acagaagaaa gtttttctcc taatttcacg cctgccaaat caaccatttg ctgtgctact
     6541 ttgttcactt tcccaggtcc gatgatgact tgaagctgtt catcttctac tacaccaagc
     6601 actcctggaa tcgcttttaa ttcagagaca tttacaagat catcattgat gatcttcata
     6661 cgcacacgag tcatgcagtg gacaatgctt tctacattgc ccatgccgcc tgccccttta
     6721 taaatttgtt ttgccagttc ttccaaagtc agttcgtttt ttgccatggt cttttccccc
     6781 tcataaggtc ccttttacaa atccgtttgc ttcagctaat tttgcttgag cttcctcttt
     6841 atcactattt gtcaaaatca tgacgatagc cagcttaaca ttttgatctg cttcttcaaa
     6901 gtacttttca gctgtttcgt aatcagcttc tgtcgcttgc atgatgattc gttttgagcg
     6961 ttcgattagt ttttcatttg ttggtttgac atcgaccatc aagttattga atactttacc
     7021 tataccaatc atactgatag tagaaatcat attcaagata agtttttgtg ctgttcctga
     7081 cttcaatcga gtagagcctg tcaaaaattc tggacctgca tcgacttcga ttgggaaatc
     7141 cgcgtgttta ctgatttcgg cttgtttatt gcaagaaatc gttcctgtga cagcaccgat
     7201 cgattttgcg taatctaatc caccgatcac atatggtgta cgaccgctag ctgcgatacc
     7261 aataactata tcattttttg tcaaattcag cttgattaga tcttcttgcc ctagctcata
     7321 agaatcttcg gccccttcga cagctacagt cattgcttct tctccaccag caattagacc
     7381 tatgaccatt tccggttcta caccgaatgt cggtacgcat tcagcagcat ctaacacacc
     7441 tagtcttcca cttgttccgg ctcccatata aatcagacgt ccaccttttt taaaggactc
     7501 aatcgtgtta gcaatcactg gttcgatact tgtcagttct ttttcaaccg cagcggccac
     7561 tttctgatct tctttgttca ttagttgaac ggcttgagaa atactcatct catctaatcc
     7621 aaaagtttct tggtttctgc gttccgttgt taggttctct aaattgattt tgctcatttt
     7681 tatcttcctt cttttcttat aaattgatag ttcataccag ctttgatttg gttgatcaat
     7741 ggtagctctt ctttgataac tttagctact acattcactt tttcatccgc tggcaaatca
     7801 tatttggtta attggatctc acccatatag cgcaaatact tttcgttatc taaagtaata
     7861 gaccctttca tacgcgtagc tgtatttctt gctgggatag gcggaatctc tcgaaaacgt
     7921 gcatctacgc ttcgtatgac atctcttgca tcgtcttgcc gattcgtatg cttccctaat
     7981 atatattcgt aaaattcttc atctacaggt tccgtatgga gcaagactgt ctgttctttt
     8041 tggaaagaag caaattgctc ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca
     8101 atatccgtgt tacttgctaa tagatctaga gcgcctgcaa gaggatggat ccctcgatgt
     8161 tcttccaaag ttggcaatcc ttgatatagt gggcctctca actctgtatc tcctggtaca
     8221 aaaccttgga caaggagacc ttgaattttg agccattgat tttttttctg ataccattct
     8281 ttgtccagcc ctgtttcggg acgtggataa taattatgcc aagcttcaat ctgtgagaaa
     8341 tcagcttctg cttctttcaa ttcatcgata tctttaggcg tgatcgtgct agcattcaaa
     8401 ctgattttca attgatgtga ccagtcggca atctgctgat tggaaatatg ataatccatt
     8461 cttaaacctg ttacaccaat tgctttcaaa ggcgttagat cttctaatga aaaacccgct
     8521 cgttccaatg cctctcctga aatatcgacc atcaattcta gtttattctt tttcgcttcg
     8581 cttcctagtt cgatcaatcg cttttgatat gccgaaacat catcttctgg tatatgcatg
     8641 gaagtgaaaa taccgacaaa gccattggat gccatttggc ggatatactg tttcttctct
     8701 tctgacaaag aatcgttcat aaatattgaa aagccaaaca tattatccct ctttttctgt
     8761 atccgtcttc atttctatga ctagagtata tatctaaaga aacaaaattt caatactttt
     8821 attaaaataa ttaaatttca tttctaaagg ttgagtttat attatcaaaa gaattgtcat
     8881 aacctaaaaa aggcactgct aagcaaaagc agtgccttga aatattttat ttaaataaca
     8941 caaaaacata attactcata agaaaccaat tctgtattat ctaccatgaa tgtaaagaac
     9001 ccctcattag gcatcggatg aaaataagca ttgatcactc ttatcaaggc attatggcaa
     9061 actaacagat agacattttc ttcgtcttcg atgcattctt tcaataaagg aacgatccgt
     9121 gcatacacat ctaagactga ctccccattt gggaatcgta cagcgaacgc taaacgtgct
     9181 ttttgaaagt tctcatcttt gcttggcagt ccatcataat ccccaaaatc catttctacc
     9241 aatcgttcat caacggtcag cggcaagctg agacgatcag caactgcttg tgctgtatct
     9301 cttgctcgtt gaagagggga atggatgatt ttcgtgattg gaacaggcaa atctactacc
     9361 ttttctgcta gtttctgtgc ctgcatctgt cctttttcag tcagcggcac atctgcccgt
     9421 ccgcaaacta atccttgtgc gttccaagtt gtttctccgt gtctcgttac ataaagcatg
     9481 tgattctctc cttcgttggc aaagttcccc ttaaccatac cttgttgttc ttgagattac
     9541 aagggttaga ttctctcaag agaaataatc tcagtagata ctttatccag gaaggtttga
     9601 tcatgttcca ctagtaacat ggcaggtttc acgttcaaga tcagttgttc taattgttct
     9661 tgattgaaga catccaaata attcaatggt tcatcccatg tatatagttc agctggctgt
     9721 gataaagatt tagccaattc cacttttttc cgttggccca tactcatctg ctcgatcttg
     9781 ttatgaaaaa catctctttc catcccaagc tttcggaggt tgttcaaaaa tgcttggtag
     9841 tctacttggt ttttctctgc aaattccgcc aacgttcctc gattgtcttc ataattttgg
     9901 cttgcataac taatgctcag atgttttggc tggtattttt ctcctataac cttgccgttg
     9961 aatgccccca gaagataatg gataatggat gactttcctg caccgttagg acctgaaatc
    10021 gccacccgct gatgaggctc gattgtaaaa tgaattggct cgaataacag attttcatac
    10081 cctaattgaa gatcttctac gcttaaaagt cgcttatggt gagacgcttg gctattcatc
    10141 gtcaaagaat cgatatattc gatatctttt aatagttttt ctttctcgct gatctgggtc
    10201 tccatccgat tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc agcacccact
    10261 gctcctttat tcactcgtct agattcagta tcgatgaatc cgacttgttt ctttgtttta
    10321 tctccttctc gggaacgaga ccattcggct ttttcagctg ctgttttctt tagcctactg
    10381 acttcttttt tcaatttttc attttgagcc atttcaaatt catcacgaag ttttttctgt
    10441 tcttcataga tagagaaatt cccttgataa agttccagtt gacttttttc gattgctaaa
    10501 acatggtcca ctacttcatc gataaatccc cggtcatggc tgaccacgat gaagccttgt
    10561 tttttctttt tcaaataagc cgctacttgt tttctaccag agatatccaa atgattcgtt
    10621 ggttcatcga ttaacgggaa atgagtgtca tccacaaata aaagtgccag taggactttt
    10681 gtcttctctc cccccgatag tgtgctgaat tctctccata agatttcagg atctgtttgc
    10741 atcaattgga gctctctttc gatttcccat atctcaaaat ccgtaatatc atttaacacg
    10801 taataggtta aacgttcttt atcttttgtc tgttgcggga aataggcaaa ttcttgctga
    10861 tggattactt gcccttgata aggtagtttg ttttgtagaa tattcagtaa agttgtcttt
    10921 cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt tagatttgct
    10981 tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg tcagattttt tatttcgatt
    11041 ttagacattt tccatcacct cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag
    11101 aggatgcaag ggttggatac acacaaaaag acccatacat cgaaaaaacc aagccaatcc
    11161 cgaaaatctg cacactgctt ccttgtctcc acaaggtaca gtctgaatgc agctttcatc
    11221 aatcgactta atttttcaat gcacggatcc ctcactttgt cctatatttg aaattacgat
    11281 agcataaaga aaatgatttt gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg
    11341 tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg ttccattgct tcttctaaac
    11401 ctattggacc caaatgaata cttaagacaa ttgggagaaa atcagaaagt ccttctagct
    11461 cttggtctct acttccgcaa atagcgatta ttggttttcg atattttttt gcaagcactg
    11521 ctacaccgta tggaactttt ccgtggatcg tttgctgatc gatccgccct tctcctgtaa
    11581 tgatgacatc tgcttgttcg atcttttttt ccaatccagt gatttggctg actaaatcga
    11641 aaccagaaac catcttcgct cccaacaaaa aaagggctcc accaataccg cctgctgctc
    11701 cacttcctgg aatatcatct aaatccacct cccagaaatc ttttatttgc tgagcaatct
    11761 ttcctgcttg tttatctaag aaagctagcg tcgtatctat tcctcctttt tgtcttccaa
    11821 attgccaagc agctccttgt tgacctgtat atggattggt aacatctgca gcgataatca
    11881 attttacttt agaaacaaga acgtttcttc gatcaaaatg aagtttttta gtagataata
    11941 aaggatttcc ttctgtgtac ccttctaaag aagcacccaa ggattggagt aatcctagac
    12001 caccatcgct acacccgctt cctcctaaag tgacaataat ttgttctact tcttgaagca
    12061 gtgcatcttt aataagttct cctaaggcat aactggaagc aagtcggata gtttgatccg
    12121 ttggtttatc aagtagatct aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt
    12181 ttccttgcca agttgtcaat aaatagctga tttttcgctg acggaataag agatccatcc
    12241 catcaatggt acgccatgtt cccctgagcg cgtaatagat tgcttctgca gtattttctc
    12301 cgccatctgc aatcggcaca ttgatttttc ttcctttacg atcttcccag ccatttagaa
    12361 ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa
    12421 tatccatctg tatccctcac tttcttctta agtaagacac tttttttcat ttttttcaaa
    12481 caaaacaatg ctattttctt ccatttttct atatactaaa agggaactca ttgaataaag
    12541 gagagaaaaa acatgaaaat cgcaatagct ggggccggag caatgggcag ccgaatcgga
    12601 ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt
    12661 gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa
    12721 ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga cttgatcgta
    12781 gcattaacaa aagccaatca attagatgat atgttttgtt cgatccaatc cattattact
    12841 gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt actggaaaaa
    12901 tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg tctagctggt
    12961 cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt ggaaccagaa
    13021 ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt aaatccgatc
    13081 tatagccata acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat
    13141 ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag
    13201 agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga aggaatcata
    13261 ctagatcaag aagaagtgta ccaacatatt gcttctactt atgatcctga caacatcggg
    13321 ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac
    13381 tacatcaatg gtgccatttg gcgaaaagga caaaaatatg atattgctac accatattgt
    13441 gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct
    13501 ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc aataaaagat
    13561 caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt ttcagtacat
    13621 aatctcttca aaatgtaggt tttttatttg ttttcaacag aggagaggtc atattcgtat
    13681 tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc tgcatcataa
    13741 aattccaata gctctgatag gattggctta aatggtgtat gcagaaattc cggtaattca
    13801 tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg ataatgagca
    13861 atcgcttgat tatccgtcac tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa
    13921 gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt
    13981 ttcaggtact cttcataagt aagatatcca ggttgttcat tccctcgatg tggaagaatg
    14041 aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc tgctcgttct
    14101 tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct agaagaaaaa
    14161 ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt tgcttccaaa
    14221 aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc
    14281 tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt ggtaaatggg
    14341 cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat cagctgataa
    14401 tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc ttttccggac
    14461 caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt tgcaactagc
    14521 agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt tcctatgata
    14581 gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat atttgtatga aaatttaaca
    14641 tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct aaaagttttc
    14701 tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat attgctattt
    14761 attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa
    14821 aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg tggagcaatc
    14881 ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc ttctgtattg
    14941 attgcttttt tgattggtgg gctgatttta tatatcgtga tgaaaggtct gggaaaaatc
    15001 gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc ttatctaggt
    15061 gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat caatatcatc
    15121 gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat tccagcctgg
    15181 gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc ggtacggtta
    15241 ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat cttactgatc
    15301 atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt ccctaccctt
    15361 cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat catcagttct
    15421 ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac ggtaagcgaa
    15481 gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt
    15541 tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa tactttagca
    15601 ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg
    15661 gatatcgtca attttgtgat tgtccttgct ttgttctcat ctatcaattc gggtgtgtat
    15721 gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc tagaaaacta
    15781 gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac cagtgttctt
    15841 tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt
    15901 tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc ctttgtactg
    15961 gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt agctgtctta
    16021 ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt attgactggc
    16081 ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt cgtattgacg
    16141 aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg agctattgaa
    16201 gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac agtctcatga
    16261 cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt tctccatgct
    16321 catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg
    16381 gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga tgtacttgaa
    16441 tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg cgttgatgat
    16501 gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa
    16561 gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct tgtttatggt
    16621 acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc ctcatcattc
    16681 tatcgctatc ctggcaagta aacgagctga tttaaaagat cctgaagtaa aaaaattagc
    16741 ggaagaaatc attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga
    16801 aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag ttatcagctt
    16861 caagaaataa gaagaagcta cttctggaac gccgtgaata aggggttgtg aaaaagctgc
    16921 cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt tcgtttggtt
    16981 aggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtaaaatcag
    17041 cgttctgacc tgagtattag agagacaaat ggaaaaatag ttcctcatat tttgccattt
    17101 tttgagctaa taccgagggt cagctgattg aacaccgttt atcgagaggt tgtgaaaaag
    17161 tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat ttttgtataa
    17221 atttggctta ataccgagaa actggctttt gaataccgtt tattcgtaaa agggattgtg
    17281 acaaaagtct tgtcacaatc cctttttatg tttttattgt tctaattgcg ctgtcttgct
    17341 tactgcttct tgcggtttca caaatttttt tgagattccc caacctgtga aggcactgac
    17401 catcgaaaac acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg
    17461 aataccaaat gtacttgcta cgaaacttcc ggcaattccc caaggcacta agtagttgat
    17521 tactgttcca ccatcttcta gcgttctacc taatacagtt ggatcaaatc ctgctttttc
    17581 ataagtttct ttaaatgcat taccaggcag aataacagat aggaattgtt caccaataaa
    17641 aatatttact ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat
    17701 ctttcttgat actcgttcca tcacggttga gatcaagcca aattcgatca acaatccacc
    17761 taatgacaaa gttagaataa tcaatgacac agtaggcatc atactttcga ttcctccgcg
    17821 gctaagcagt gaatcgattt gttggtttcc agtatttgaa acaaaccctg attcgatgac
    17881 cccagccaac ttcgttaagg aagtttgtgg gttttgaata aaaatcattc ctactgctac
    17941 agcgatattc aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac acagtaacat
    18001 taaaaataaa ggaatcagtg accagattga aatagtaaag tttgcttcta gaacatcagt
    18061 cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa
    18121 aaagaaagaa acaacaaatg ccgggacagt tgaccacatc atatgcttga tgtgtttgaa
    18181 caaatcggca tcaacaatag ctgctgctag atttgttgat tcggataatg gagacatctt
    18241 atctccaaaa acggcacctg aaacaatagc tccaacaacc agcgctggat ttaagcctag
    18301 tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa aggcactccc
    18361 tacagcgcta cctacgattg cacacacaac aaatacagaa gggacaaacc actggacact
    18421 gatcaatttg aacccaatga ccattagagt tggaatgatc cctgcttgta tccatacagc
    18481 aatcaatgaa ccgactaaaa taaaaataaa gattgggatg attcctggtt tgatgccatt
    18541 gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat acaacatgac
    18601 cattccgatc gctgctaaga tcgtgatatt aggtgagacc tttaatccga caacgccaat
    18661 cgcaatgcct aaaacaataa ccattaatac aagtgttgct tctaaaaaac ttggtgttcc
    18721 tttttttccc atgataatct ctcctttttt agttgttctt atgttctaaa aaagttaaga
    18781 tatcggatta ctccgccgca attcatccgc gattcctcct ttataaaaat aaaaagacct
    18841 gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca gatctgtttt
    18901 tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca attaggcttg
    18961 atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac tgcgtgaaaa
    19021 gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt
    19081 tttcattttt ctgaaaatta tttttataac tttcacacac aagattattt tttactagaa
    19141 agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct tgattctttc
    19201 taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt ctgctgcaat
    19261 cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc
    19321 ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga cttggctgac
    19381 atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt cttcatctgg
    19441 aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag taacgatgac
    19501 atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc
    19561 acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc gatttcctgc
    19621 acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc
    19681 tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag cgttgattcc
    19741 attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg ctgctgctgc
    19801 atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag attctgtcgt
    19861 tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct caaaaaaacc
    19921 ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc ctggggtgcc
    19981 atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc tggcggctaa
    20041 tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg gtaaagcatc
    20101 gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac gtgcatcaaa
    20161 ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt ctaattgttt
    20221 aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt cgtggatatc
    20281 tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca attggtttgt
    20341 tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt attttctccc
    20401 gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt
    20461 gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt catcttcttt
    20521 tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt ctaagaagat
    20581 ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga tcactgaaat
    20641 gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa tgtctagatt
    20701 ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct tatccacttt
    20761 gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa catcattagg
    20821 gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt tagaataaat
    20881 tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt gttctttatt
    20941 gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc catctgctaa
    21001 agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa tgttctcctt
    21061 tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa tccctagttt
    21121 tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc ctgccaacca
    21181 aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt tttctcctcc
    21241 actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt aaaaaaacta
    21301 agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga ttataccctc
    21361 tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg gtagtcagtc
    21421 cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa ccttctaaca
    21481 aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc gcttcgatca
    21541 ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac ggaacaactc
    21601 caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact aagaaacgag
    21661 tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg atattgatca
    21721 gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg cgtgtcacta
    21781 ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc caaaaagaca
    21841 tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa acattgggca
    21901 aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt gatacgacta
    21961 tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa ataatccaat
    22021 gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc taacggcgtt
    22081 tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata tttgctgtac
    22141 agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc caccagccat
    22201 ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca
    22261 agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt gctgaagatc
    22321 tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac cttgttgcag
    22381 atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc taactggcga
    22441 ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag tgcactggtt
    22501 gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt
    22561 tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc caaaccgaca
    22621 agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc agaatacttt
    22681 aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa atgctgaaag
    22741 atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg actcagattc
    22801 gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat cacacgaact
    22861 aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc tttttcaatc
    22921 gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa acatacagaa
    22981 acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa
    23041 tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt
    23101 gttcacacac aactcctctt caagtacgat catactctag caagatatcg gtcgcagccg
    23161 tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt
    23221 ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat
    23281 ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta tgtcagtaaa
    23341 aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc
    23401 tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat
    23461 cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca
    23521 gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg
    23581 ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa attatttttt
    23641 ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa gatcttttcc
    23701 ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga caaaactgga
    23761 atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga
    23821 atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag ctgtcagata
    23881 aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg tgtccaagta
    23941 atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc
    24001 aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg catatggttt
    24061 taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct tcgctcggac
    24121 ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac tttgtttcca
    24181 gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa attgctgaaa
    24241 tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac catctaaatc
    24301 aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca tagtttagca
    24361 cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa gagttcttgg
    24421 aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc gatgactgta
    24481 gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc agcaccaaaa
    24541 cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac aaacatggca
    24601 aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg gagcactaag
    24661 gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat gaaaatcact
    24721 cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca aataacaaaa
    24781 tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg
    24841 aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc tttatccatc
    24901 cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa caaaagttgt
    24961 aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga aaacatattg
    25021 ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa agtttctcgt
    25081 ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca
    25141 gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc cactttcatt
    25201 gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt tttacgtaaa
    25261 gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc atggtcttca
    25321 gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt aaccaattca
    25381 ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt gtccccagtc
    25441 agaactttta cagctacacc atgttctttc agagctttca acgcttgttt cgttgtttct
    25501 tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc atctttgact
    25561 gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac tcgtaagcca
    25621 tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt tgttaatggt
    25681 gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc aatcgcacct
    25741 tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat acgacgacgt
    25801 tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt atctaaagtt
    25861 tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag
    25921 ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga
    25981 tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca taacacatcg
    26041 attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt tttggccatc
    26101 acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggtgttaga
    26161 ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt tgtaagccca
    26221 ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag catggatgtt
    26281 ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt ttctgctaca
    26341 tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact tccactgatg
    26401 acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg atcgtcttca
    26461 catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc ttgggaaatg
    26521 aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc tgctgaaagc
    26581 cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt tccattacgt
    26641 tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg attggaacgt
    26701 acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat aatggctaca
    26761 ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa tgaaataatt
    26821 cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg
    26881 aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct tgaacgttcg
    26941 atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa ttcttgtttc
    27001 gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc catctcaatc
    27061 tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc tattcatcgg
    27121 atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc tggcctttcc
    27181 tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta
    27241 attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt agaccacctc
    27301 ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc caaatactca
    27361 gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt tgagttttga
    27421 cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa gccttatctc
    27481 gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg
    27541 tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata acaactaaaa
    27601 aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata cacacgttat
    27661 attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata atagaaatat
    27721 agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc agacggcgtg
    27781 agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact ggccattgct
    27841 gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca gcacttgact
    27901 gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt cgtttatctt
    27961 gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc aacaaaagct
    28021 gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac tgaaaacatc
    28081 attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca
    28141 ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta aactgctggt
    28201 ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc gttgattctt
    28261 tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc
    28321 taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat agatcactgt
    28381 acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac gtgccttttt
    28441 caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa agagatactg
    28501 ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca aataattgtt
    28561 ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata gttcaaattg
    28621 accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa
    28681 ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca aaaccaaata
    28741 atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt tataaggatc
    28801 aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact ttattggttc
    28861 atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc gctctaaaaa
    28921 tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc cgactagaga
    28981 agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt tgactcgaag
    29041 tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa atttttccac
    29101 attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt
    29161 ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga aggttggttt
    29221 gattgtttat tgattatttt tttgctgttc aacgtctgtt cttgttactg gatataagtt
    29281 ggttgttgtt ttattaccat tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt
    29341 ttcgattgct tcattacgtt ccgcttcatt tttatagtca tattttttcg gatcaatcgt
    29401 ctcaaatccg tttggcacat aaaaccgcaa gagattttcc tgattcagct tatctgaaag
    29461 acttaacgca gtcttgactt tttcctgatc tttggagatt tcctcatcga ttcctgctgc
    29521 tttcgtatcg atgatttctc ctgtctgatt attgtaagct tttcctccta ggacggtgta
    29581 tttgggtgtc acaaagttcc catttcgaaa agcaactact tgatcatgct gagaagataa
    29641 taagtctgta ccaaaatgaa gataatcttt atcatcgatt cccaatagat gtagtaatgt
    29701 cggcaaaaca tcgatttctc caccgtattc atgattgact gtccctttag tataaccagg
    29761 catatggatc atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc
    29821 tggatcttta ccaagagcgc ttgctaaatc tttattatct gtattcgaga tcccaaaatg
    29881 atccccgtaa atcacaaaga ttgaattttg gtaaatacca gattttttca aatacgtaaa
    29941 aaattgttcc aatgattgat ccagataatg tgccgttcga acataattat ctactgtgct
    30001 attgccagta ttcaaagatg gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg
    30061 gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc
    30121 agattctctg aataaatctt tatctaatat cccataacct aacgtttcgt cggattcatc
    30181 aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat gatctctatt
    30241 ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct tttgtccaag
    30301 gatcgctggt gcggcttgaa agacattgtc tgacccaagt tctgtgaata atgagccttg
    30361 aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct ttccctgtcc
    30421 tacttcatga aagaaattgt caaagctgat cgttgcttga ttctggaaaa tactatttaa
    30481 aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct ggaaactttc
    30541 taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg gatttggctc
    30601 tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc
    30661 gcgaacactg ctagtcattc ctgttttgat tccgtcataa atagtatacg catcgattcc
    30721 tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg gacggttggc
    30781 ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc
    30841 catcggtttg ccgacagggt tgcttttgat aggtattttc ttgaccttca accaaaccag
    30901 tagcccaatg aacacagcga tatccagcca gtagatgaca tcatggggct tcatcaaaga
    30961 aaaggagctt cctgacaatc cttgcgacac tttcgaaaag cctaatacag atttgactgt
    31021 aatgaaatct gtgaattccc gataaaaaat aatattcaag tacaacaaaa gcgtatttaa
    31081 aatatcaatg atcaaaagaa tagctaacgc tggctttggc tttttaatat acaaagaaag
    31141 gccaaaaaac aataaggtag tcgcaattgg gttgatccac aagataaagt actggatcgt
    31201 accttccaca cctaatgaaa aatctgcata gtaggcaatg atcgtcttca gccagaaaaa
    31261 gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac
    31321 gtatatcact tctctctatt cacatttttt cttattttgc tcctgaaaaa tgaaaatttc
    31381 ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc cgtccacttt
    31441 tcacagcata aaaccgctgc gatgtaagcg attaaactaa tgaagagata aaagtcttgt
    31501 ggtgaccgct tcactccacg gtattcaaaa gccagtttct cggtattcgt acaaaaaaag
    31561 aggctgtgac aaaattcttg tcgcagctcc ttatttcttg atgcaggaca gctttttcac
    31621 aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca caatatttat
    31681 aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa acggcaggct
    31741 caagacctct tgatacacgg tgttcaatca gctgaccctc ggtattagat tatgtcagga
    31801 cgctaatttt acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    31861 ccaaccaaac gaaaaatata aggagctatt ttttgaaatt gctccttgtg gctcagagtt
    31921 aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgacct tcggtattag
    31981 ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt gtttctctaa tactcaggtc
    32041 aaaacgctga tttcacaacc tctgtttttc attgtccata ggattgatat tgtccttgtg
    32101 atgactcatt cgtaataatt gaggcttgat tttcttcact tcccgttatc gtttgtccac
    32161 tgccagaatc tgaaggactt gttcctgtcg actcgttttt ttgagaagag acagttgttt
    32221 tcttgacttg ttcatttgtc tttttccatt ccgctaaacg atcggcgacc acttttttgt
    32281 ctactttttc agaataagcg gtgatcactt ttccattttt caaacgggct actttgtcgc
    32341 catcatgagt caaaagcaaa acggtcaaat gcttgtcttt aaattgatat tgtctgagag
    32401 aaatcgcact aagtacgtca tcgatattaa taaattgatt ttcttctttt ccttttggca
    32461 aataaatgtc caattcgcct ttttcgtaat tggcagttat tttatactcc gcccaccaat
    32521 aagttgagaa ttcttttttg aaattatcta ctgattcttt gcttgctacg attttttctg
    32581 tttctttctg agcaagatct gctgtcattt ttcctgaaaa tgctgcggat aaaataagca
    32641 gtaagaaaaa accgaataac caaaagagaa gaaacggtct ttttttcctc atgctttcct
    32701 ccactcctat tta
//
LOCUS       DAZKPO010000026        31704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000026 DAZKPO010000000
VERSION     DAZKPO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.026"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG8NZ_001166"
     CDS             complement(1016..1357)
                     /locus_tag="ACG8NZ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0538860.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG8NZ_001167"
     CDS             complement(1357..1587)
                     /locus_tag="ACG8NZ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0538861.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACG8NZ_001168"
     CDS             1874..2212
                     /locus_tag="ACG8NZ_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538862.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACG8NZ_001169"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACG8NZ_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACG8NZ_001170"
     CDS             complement(3711..4292)
                     /locus_tag="ACG8NZ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0538863.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACG8NZ_001171"
     CDS             complement(4475..5101)
                     /locus_tag="ACG8NZ_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0538864.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACG8NZ_001172"
     CDS             complement(5123..6454)
                     /locus_tag="ACG8NZ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0538865.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACG8NZ_001173"
     CDS             complement(6609..7010)
                     /locus_tag="ACG8NZ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0538866.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACG8NZ_001174"
     CDS             complement(7096..7302)
                     /locus_tag="ACG8NZ_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538867.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACG8NZ_001175"
     CDS             complement(7397..8899)
                     /locus_tag="ACG8NZ_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0538868.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8NZ_001176"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8NZ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0538869.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACG8NZ_001177"
     CDS             complement(9470..10087)
                     /locus_tag="ACG8NZ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0538870.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACG8NZ_001178"
     CDS             10308..11681
                     /locus_tag="ACG8NZ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0538871.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACG8NZ_001179"
     CDS             11756..12868
                     /locus_tag="ACG8NZ_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0538872.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACG8NZ_001180"
     CDS             complement(12940..14577)
                     /locus_tag="ACG8NZ_001180"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0538873.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACG8NZ_001181"
     CDS             complement(14745..15353)
                     /locus_tag="ACG8NZ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0538874.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15737..16888)
                     /gene="queG"
                     /locus_tag="ACG8NZ_001182"
     CDS             complement(15737..16888)
                     /gene="queG"
                     /locus_tag="ACG8NZ_001182"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0538875.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16907..17659)
                     /locus_tag="ACG8NZ_001183"
     CDS             complement(16907..17659)
                     /locus_tag="ACG8NZ_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538876.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17800..18750)
                     /locus_tag="ACG8NZ_001184"
     CDS             complement(17800..18750)
                     /locus_tag="ACG8NZ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0538877.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18751..19590)
                     /locus_tag="ACG8NZ_001185"
     CDS             complement(18751..19590)
                     /locus_tag="ACG8NZ_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0538878.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19587..20333)
                     /locus_tag="ACG8NZ_001186"
     CDS             complement(19587..20333)
                     /locus_tag="ACG8NZ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538879.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20571..21941
                     /locus_tag="ACG8NZ_001187"
     CDS             20571..21941
                     /locus_tag="ACG8NZ_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0538880.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21996..22892)
                     /locus_tag="ACG8NZ_001188"
     CDS             complement(21996..22892)
                     /locus_tag="ACG8NZ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0538881.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23006..23176)
                     /locus_tag="ACG8NZ_001189"
     CDS             complement(23006..23176)
                     /locus_tag="ACG8NZ_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538882.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23173..23850)
                     /locus_tag="ACG8NZ_001190"
     CDS             complement(23173..23850)
                     /locus_tag="ACG8NZ_001190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0538883.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23941..24846)
                     /locus_tag="ACG8NZ_001191"
     CDS             complement(23941..24846)
                     /locus_tag="ACG8NZ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0538884.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            25021..26475
                     /locus_tag="ACG8NZ_001192"
     CDS             25021..26475
                     /locus_tag="ACG8NZ_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0538885.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            26491..27522
                     /locus_tag="ACG8NZ_001193"
     CDS             26491..27522
                     /locus_tag="ACG8NZ_001193"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0538886.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27529..28716
                     /locus_tag="ACG8NZ_001194"
     CDS             27529..28716
                     /locus_tag="ACG8NZ_001194"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0538887.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28872..30254
                     /locus_tag="ACG8NZ_001195"
     CDS             28872..30254
                     /locus_tag="ACG8NZ_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0538888.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30291..30611
                     /locus_tag="ACG8NZ_001196"
     CDS             30291..30611
                     /locus_tag="ACG8NZ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538889.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(30811..31479)
                     /locus_tag="ACG8NZ_001197"
     CDS             complement(30811..31479)
                     /locus_tag="ACG8NZ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0538890.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8981 a         6422 c         5714 g        10587 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tcataggtaa aagaaacatg acatttaatg acaaaataac gaattattta atatttgtaa
    15481 catttaaaca aatatttttt tgatacaaaa ccataaaagc cttgttgtat aagcaaagta
    15541 atcgtttttc tatgtttttt tatttttgaa tgaaaaacgc agattctgta acattcactg
    15601 caataatcca taaaaaaaag caaaaaaatt tttgattttt atcaaattct cttaaaaaaa
    15661 tgtaaaaaat acaaaaaaac cagtaattgt tacaacaatt actggacaaa aactttgaat
    15721 aatgttaact atttttttat tttaactttc tccgcaataa tttctgggca tgttccaatt
    15781 cgtttataac gacttcttct gtttctttag ctaatatttc atctaataca tcaagccatt
    15841 tttccggttc ttttgttcct agacgtccaa tagcccaagc tgctgttcct ctgatgacag
    15901 ggcggacatc tgttaaacat tctgaaatct caggcaatgc cgttcgatca cctagattag
    15961 ctaaagcaat caatgcgttt ctctgcaaag gtttcttccc acgccatgaa ccagctaaat
    16021 gcccaaattg ctgtttgaag tctttattag acatcgaaag cattggcttt aattttggat
    16081 atatttcgtc tacttttggt tccatctctt catgaaaatg aaaatctttt ccttgattgt
    16141 aagggcagac taactggcag atatcacatc cataaatgac atttcgcatt tttttacgat
    16201 attcttccgg catcatccct tttgtctgag tttgataaga caaacatcgc tgggcattca
    16261 ttcttccgtc accaagcaat gcttttgtcg gacaagcagt cacgcagcgc atacagtcac
    16321 cgcaaccaaa ttctcctggt ttatcaggct caaatacaat attagtaacg atctcaccaa
    16381 gatagacaaa tgaaccgaac tcttcagtga tcaacaatcc gtttcgtcca ataaatccta
    16441 aaccagctcg ctgtgctaca gcgacatcga tcagttctcc agtatctact tgcggagcta
    16501 gacgccattc ctcttctttt tgccattttt ctgctcttgt ttggatgaat tcgatcaatc
    16561 ggttcaaacg gtctctgagt atatcatggt aatcgatccc ccaagaagca cgagcaaact
    16621 gcccgcgttt ttcatcccta ggcacttctt catggatcct agtaggatac gcaagtgcaa
    16681 tggaaatgat cgatttcggc tgatcaaatg tcatttctgg ataaagacgc tcatcgatat
    16741 ttggatgttc aaagccagaa gtatggccag ctgctttttg ttcttccaac gattctttta
    16801 atgaatcaaa aggttccgcc gtggtgaacc cgattttgtc aattcctaat cgtttgcttt
    16861 cttgtatgat ttcttctttt aatgtcatgg tcttcctcgc ttgctgttaa cggctgtata
    16921 acccataata tagtttttct tttttcagct ggacaaactg gatctgctga tcggcaatct
    16981 tcttttcttc catccatttt tttcttgcag caaatacttc ccaataattt gttgaaatta
    17041 attttctttt gttatctaat gcattaaaaa aattcaatga aactggtttt gtacttaaca
    17101 gttcatggag ccttacctct tcccatggcg aaaactcaga cagatcgcgg gaatacaata
    17161 cttcttcata gagatttttt tgctgtgtgt acgtgaatgt ttgcgggatt ttcccaggcc
    17221 tagcaatttc tactagacga ttataaaaca tattcattaa tttgctgttg gcaataaatt
    17281 gacttttata ataggccgac tggatctttt tgattactcc ttctgctttg atgactgaaa
    17341 ataatgcagg ccatgctttg acggttattt cgtcgttttg tttaactaat acgccaggtg
    17401 tgaagccgac tggagaaagg aacacaaaat aaccgatacc ggtattttca tctaaaggct
    17461 gaaattcatc aaacaaccat ttgcctatgt acagctcttc atctggttct gttgtctcat
    17521 tttgatttgc tatgaataat ttagctaatt ctgcttcaag aaattgaagg tcttgatcct
    17581 tgtcggtatt ttcctgctgg ctgatcaatc gaaagacttc ttctttaatc gtatatttat
    17641 ctttacgtgt aaaattcata gccacacctt ccttccttac tctaactata acggaatttc
    17701 ttcaaaaaag gtatctgtac gacgaaaaac agcataattt tttttcatat ctcttataaa
    17761 aaatgcaagg tctgctcctc aagctagacc ttgcactttt cagctttctt ttgtttgtga
    17821 caaaccttca tggatcgttt caatattcca tttcatcatc tgatagtaag aatctccttg
    17881 ttctccttct tttgctatgg aatcagtgaa cagtttatca tagattttca aacccgtttc
    17941 ttttgctacc cgttccatgc ttcttgagtc tacactggtt tccacgaata aaaccggtac
    18001 ttcttttgct cttatctgat caatgatcgc tttcatttga tcaggcgtac cttgactttc
    18061 tgtattgatc tcccatatat aagctgctgg caaatcatat gctcgtgaaa aatacttgaa
    18121 tgccccttca cttgttacta ataatttttt gttctcttcg atagaagcaa aactttcctt
    18181 cgcttgtgta tctaattctt ttagtttttc cacatatgct tctgcatttt ttttatagag
    18241 tgtcgcattt tctgcatctt tttcagagaa tatacgtgcg atttcctcca catattttat
    18301 tccgttagat aggtctagcc aagcatgggg atctgctttt gtatgttctt caccgctagt
    18361 taaataaaga ggttctacat ttttgctaac tgcaaaataa tcttttcctt cttctttttt
    18421 agccgtttcc atcaagttat cgaaccagct gttacctgtt tcaagattca aaccgttgta
    18481 taaaataaca tctgcatcgc ttgccttttt gatgtcttct ggtagtactt catattcatg
    18541 cggatctgtt ccgactggta cgatactgtg gatatctatt tgatctgttc cgacctcttt
    18601 tgccatgtcc gccaagatcg aattggtagc cacgactgac aacttttctt tacttccttc
    18661 atccgcttgg tcagaagcct gctttccgca accggctaat accaagaaac taactgccaa
    18721 gattaaaaag agtgattttt ttgttttcat ctatctgctt cctttctgaa ataaaattcc
    18781 ttttgttgga gaaaataaaa aagcaaggat aaaaaatgcg gtacatgcta acacgatcgt
    18841 agctcctgat ggccaattgt aactataact gaaaaacaca ccgacaactg cacttagcat
    18901 accgattcct gcagctgtaa tgatcatttt cagtaaatgg ttcgttagca agtaagccgt
    18961 ggcggctggt gtgatcaaca tggcaatcac caagattgtt cctactgtct gtaaactgac
    19021 tacagccact aatgtcaaaa agaacatcaa aagataatgg atcagcgatg tgttcagccc
    19081 ataagctttt gccattgtcg gatcgaatga tgtgatcttc aattctttat aaaagaaaaa
    19141 gacaaatatc aagacgattc cactaacgag tgctgtcagc aacaaatcgc tttccctaac
    19201 agctaagaca ttcccaaata aaatatgata caaatctgtc gcactctggg catatgaaat
    19261 caagataatc cctaatgcaa aaaaggagct aaagacaatt ccaatcgctg tatcattttt
    19321 caatcggctc cgctgagtca cgaaaccaat cactgctgct gttagcatcc caaaagcagt
    19381 ggctccaaag atgtaactaa aaccaaacat ataagaaatg gctacacctg gaagaactgc
    19441 gtgagagata gcgtctccca tcaaagacat ccctcgtaaa ataataaaag aaccgatgac
    19501 tccagaagca agtcctacaa gcatggaagt gaccaaagcg tattgcagga attgataatt
    19561 cattaatcca tcaataaagg attggatcat tttttctctc ctccttttac taaatgtctg
    19621 atgatctcac cataggccaa ttgaaggttc ttcgatgtaa agacttcttg tactggaccg
    19681 gaagcgatca gctgtttatt taaaagaata acctcatcga aatattcttc tactttatga
    19741 agatcatgat ggacgatcaa aatcgttttt cctgaattct tcaattcctg caagatgtca
    19801 aagatctttc gttcacttaa cgcatcaatc cctacgaatg gttcatctaa aaagatccat
    19861 tctgctcctt gggctagagc tcttgcaata aaaactctct gaagctgtcc acccgatagt
    19921 tcgctgatct gtcttttggc atattcttcc atccctactt ttttcaaagc ttgtcttgca
    19981 cgttcttttt cttttttccc aggtctttgt ccaattcgta aagatggata tgttcctaaa
    20041 agtacaacgc ctaatacatc aattggaaag gaaagatcca attcacttcg ctgttccacg
    20101 taggctattt ttttacggac tgagtttacc gactgtccag aaaaggtgac tttaccagta
    20161 gtagctggta tcaactctag caaagatttc ataaatgttg attttcctgc accgtttggt
    20221 ccgataatac cggtaatttt ttgtggattt atcgtcaagt tgatagtttt caaaacagtt
    20281 ttcccttgat aagcggctga aaggtcttga attacaatgg ccgtacgttc caaatcttac
    20341 actccttttt aggtttacct aactttttat ttaaatatag tttaactaaa atggaaagca
    20401 ttttcaagta gaatgatgaa aaagtttttt tcgaattttc ctcgcatttt atgaaaattt
    20461 tctgtagaat cacatcaaga gttcaataag gctaacctaa taatgaaaaa catgtgttga
    20521 aacgtttgat tattttttct tgttgactca acaacgaaag gagtttttgt atggaagaag
    20581 aaaagttaac tcgaagtctt agtgcccgtc atatccaaat gatcgcacta ggtggaacga
    20641 tcggtgtcgg tctgtttatg ggtgcctcct caaccatacg ttggacaggg ccctcagtga
    20701 tgggagccta tgcgattgca ggattctttt tgtatttgat tatgcgtgct ttaggcgaaa
    20761 tgctttacgt tgatccttcg actggttctt ttgcaaatta cgcgagcgag tacatccatc
    20821 ctgtggctgg ctacttgacc gcttggagta atatttttca atatattgtc gtaggaataa
    20881 gtgaagtgat tgctgtagga tcttatatga attattggtg gcctgatttg ccggcaatta
    20941 ttcccggaat cattgtcgtc ttgtttctta tgctggcaaa tcttatttct gtcaaggcgt
    21001 ttggcgaatt agaattttgg ttttcgatca tcaaagtcat aacgatcatt ttgatgatca
    21061 ttgcaggtct tggggtaatc ttatttggat ttggtaatca tggtcaggca gtagggattt
    21121 caaatctttg gaaaaatggt ggattcttca ctggaggcgt caaaggattt ttctttgcat
    21181 tatcgatcgt tgttgcttcc tatcaaggaa tcgaactgat cggaatgact gctggtgaag
    21241 cagagaatcc taaaaagacg atcattgaag ctgtccagtc cactatcggt cgaatcttga
    21301 ttttttatat tggtgctatt tttatcattg tgagtatcta tccgtggaat caattgagtg
    21361 agattggttc accttttgta caaacttttt ctaaagtcgg cattacattt gctgcaggac
    21421 tgattaattt tgtcgtgatt acagcggcat tatcaggttg caattcaggg attttcagcg
    21481 caagtcgtat ggtctataca cttgccataa atggaaagat gtctaaaaaa tttctgaaac
    21541 tatctcgtca cggtgcgcct ttttatcctg tcgtagcaat ctcaggtgga atcttaattg
    21601 gtctgatctt aaattatctt ctaccaatgt tcatcaacca gtcagaaaat attttcgttt
    21661 tcgtatacag ctcaagtatt ttgcctggta tggttccttg ggtagtgatt ttgatcagcg
    21721 agattcgctt caaaaagaag cacgcagata aaatgcatga tcatcctttt aaaatgccac
    21781 tatctcctta tacgaattat gtgacacttg cttttttagc tatcgtattg attttcatgt
    21841 ttatcaatcc tgaaacgaga atctcattga ttattggact cgttttcttg ctttacatga
    21901 cgatccatta ctttgtacgc gaaaaaagaa ccggaaaata aaaaaagaac gaagcttcac
    21961 cttagtggaa gcttcgttct tttttattgc ttttatcaat tcaattttag cgtctcaccg
    22021 ggagtcagga aataattctc catgctcatc ccattcagcg acaagatcgt ttctactggt
    22081 acgccagttc gttcggctat ttgatttgga ccttctcctt ccaaaactac ggttgtcgca
    22141 gcctgtgaag tcgcgctgtt ggtattagtt tcagaggaat ctgttgtgtc ttgtgttgtt
    22201 cgagcttgca tttcagaagc ttctaccgac tctgctgtct cagaatcaga agatgttgtg
    22261 tcttgtgtgc ttgttgactc tgcttgaaga gaggcgctag tctgcaaata aatatcggaa
    22321 atcttagcga catgattcgt ataattagcc gataattgat tgatcaaagt atctaagtct
    22381 acaccgtctt tattttctgc tgcctgatac gctgcacgta attttctgat cccttcgtcg
    22441 gagattgctt gaagctcacg tgctttttgg ttagcaactg ctgataaacc agctattccc
    22501 tcttgatttc cctgaatttc tgtctggtat tcttcaatca atgcaggcgt cttttcttcg
    22561 atctttttcg aataatcatt tacgattgct tgaagttgct ctggcaaacc atttgctgtt
    22621 ttttcttcag aagtttgtgt ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc
    22681 tctgttttct ttgtcgttgt ttccgttatg gtcgtacttt tcgcttctga cgaatttttt
    22741 ccatgaaaat atagacccgc aactcctcct gctatgatca aaaacacggc aactgctgcc
    22801 ccaatcatga tcatccgctt acgagctgcc tgtttttgct gtcttcttcg ggtcatccga
    22861 acattttctc cccactcatt ttcatcatac atttttttca ccaccaattt ttatgtataa
    22921 tagcgttatc aaacgcttca tattcttatt gtagcaaata attgtcgctg tgcacatttt
    22981 tatctttgtt atttataaga aaatttcacc agttgagatg attccaagat tgtccgtcga
    23041 tttctccttg cagctgcatt gttcgttcgt ctatctcagc catcagtttc tttatttcat
    23101 ataagagcgc acggtcttga tattctgatt tttcgcttaa ttcatctaat tgttggatca
    23161 gccaatcatt tgtcataatg aagttccttt catttcttgc agtgtaaagg tcaagtcttc
    23221 gatctgctct tttgcctcag tcagtgcttc tacgatatat gcttgttttt cagattcttc
    23281 ttcctgcatt ttctgatttt cctgtactct ttgaataaac cagtcaatcc gctgagtcag
    23341 cgatggtttg ccttccaatg agagatactg ctgataagca attagcttct cccaacgttt
    23401 ttcctgggat aaatacataa attgtgctaa aacgaatggc ggaagatagg tcatatggaa
    23461 tttatttgcg attcgctgat acggcctcaa aaattcggaa atcgtatatt cttcttttcc
    23521 tccagcttgg tattcttttt ccgccacacc tgttgtcaat acgattccta gttctttccc
    23581 ttttaataca tgtgctgctc gttccaagac attatcttgc caaattttta aatgagcagg
    23641 agaactatac cagtaaaacg gaaactggaa aatgatccga tcatgctggg ccaagagctg
    23701 tagttcagtc tctatatcta tttttccgtc tggataactt gattccaaat gatgccaagt
    23761 agctggtaaa ccagcagaag cttctttgaa aaattgctga tttaaagatt cttcgatcgt
    23821 tggatggcta atgatgataa gtgtacgcat cccttttcct cctttttagt cttcgcttta
    23881 cagtgtagca taaccgccac aagtttcttt tgccgatgct tgtcatttta accagactaa
    23941 tcaggttgtc caagaaactg cggtttgttt ttccaactgt tttttcaagg tatccaaagc
    24001 aatattctta aaattttcgt ttttcatacg tttacgatag ttcgtagccg tttctccggt
    24061 actctttttg aacattcggc taaatacgac tagactattg aaccctactt gataggcaat
    24121 actttccacg gattcatccg tcagagaaag aagaaacgcc cctcgattga tccgtacccc
    24181 gatcaaatat tccttgatac tggtacccat atgatttttg aatactcgcg acaaatggct
    24241 ccgattgacc gctaaatgct gggcgatttc ctcaacagac aatttttcag cataatgtgc
    24301 ctcgatgtat ttgactgttt caacagctaa agaggaatga accttttgta caccaccgct
    24361 gacctttccg ccatctttta aaaggactga taaaaaacgg taggttaatg ctgttaactg
    24421 taattcattt tccaaggttt cttccgtatc tttcatgcat tctaaaataa tgtcgatata
    24481 tttttgtatg tcagaagaga ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat
    24541 gatttcttct gcagcactcc caccaaatga aagccagcaa tacatccacg gatcttctcc
    24601 atctgataag taaaacgtcg agtctcctgg acgaatcaag aacagatcac cttttttcaa
    24661 ttggaattgt tgatttttca catgataaga tcctttgcct tccatcacga catgcaggat
    24721 gtaatcattg cgaatagcag gaccaaaaga atgaaacggc atcgttttag agaaaccaca
    24781 aaaatcaaaa tacaattctt tttctacttg ttgatgttcc ttttttaaaa aaatcgattg
    24841 ctccacgtct atccctcctt actcctttag tataatgcaa caaaaagtta aataaaagtc
    24901 acagaaatat attaaaaagg aagcgatttc ttgttaagtt taagtcatta aaagatcttt
    24961 gttattcgtt gtaaccggtt tacttagtat tatatattga acaggagagg aatacagatt
    25021 atgaaaagca aaatgatcca acgtgtgtcc tacgctttag gcgcttttgg acacgacaca
    25081 ttctatgtta cattaagcac ttattttatg gtattcgtga caagccagat gttcgcagga
    25141 acagataagg caacgaatgc aaaaatgatt gctacagtta caagcttagt tgtaggtatc
    25201 cgcttagttg aaattatttt tgatcctttg attggtggca tcatcgataa tactcgtaca
    25261 aaatacggta aattcaaacc atggttagtt atcggcggcg ttgttagttc cgtactcttg
    25321 gtagcaatct ttaccaattt ctttggctta gctactcata atacgacttt atttatcata
    25381 ttatttgcaa tcgttttcat cttattggat agtttttatt catttaagga tatcgctttt
    25441 tggtcaatga ttccagcttt gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt
    25501 gctcgtttcg gctcttctct aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt
    25561 actttcttta cctatcttgt tactcataaa catattgaag gcgcttcagg ctggttctgg
    25621 ttcggtgtga ttgctgctgt tatttcaggc ggtactgcga ttatcactgc cttgggcaca
    25681 aaagaagtcg atactgcgat ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc
    25741 aaagcagttg ctcaaaatga tcagttgatg tggcttgccc ttgcttatat cgcttatgcg
    25801 attgccaatg cagcaacaac tgccgtatta ttctacttct tcaaatatgt acttggccaa
    25861 ccaactgaat tttggatcgt tggtctagtt gctgccttac ttggtttagt agctgttcca
    25921 ctattcccaa tgcttacacg cgtcatctct cgtcgctacg tttaccttgg agggatcgca
    25981 atgatgattt cttcttactt tttctttacg atcgcaggtt caagtttgcc aatcgttatc
    26041 atcgggctag tattgttcta cttccctcaa caattgattt tcttatccgc tttgatgacg
    26101 atcacagact ctgttgaata tggtcaatgg aaaaatggta tccgtaacga agctgttact
    26161 ctatcaattc gccctttact agataaaatc gctggtgctt tgtctaacgg gatcgtaggg
    26221 tttgtcgcta tcgcttctgg gatgactggt aatgctactg ctgctgatat cactgctcat
    26281 ggcgtaacga ctttcaaagc ttatgcattc tatgctcctg ctgctttgat gattttctcc
    26341 gctattattt ttgcttggaa gattacattg acagagaaaa aacatgcaca aatcgttgag
    26401 aaattggaaa acaaattggc tccagctgat acaatggaag atgaacttca cgatgcagta
    26461 gatccttccc attaagaaag gatcttgaaa atgaagatta cagaaaaaga ttttggccaa
    26521 ggttatcact tgataacctt ggagaacaaa aacaagctag cactttctat ctctgattta
    26581 ggtgctagaa tcgtcagttt aaaatcaaat gatcgagaat tagttttggg atttgacacc
    26641 gctgaagaat atatcgaaaa agatccgtat atcggtgctt ctatcggtcg caccgcaggg
    26701 agaatcgaaa atggccgctt ttctctcaat ggaaaaactt accaattagc cacagatcca
    26761 aaaactggac ataatctgca tggtggtgct cctggatttg aattaaaaaa atggtcttat
    26821 gtcattttga atggcgaaaa tgaagcttct gtcattttta ctactacaag tcccgacgga
    26881 gaacatggtt ttccaggtac aatggatgtg gaaatccgct atactttgac aaaagataat
    26941 atctggcgag tgacctctcg cggcaccagt gaccaagata ctttattcaa tccgacaaat
    27001 catgtttact tcaacttgac gggagatgct tctcagtcaa tcgatcaaca tgagctttgg
    27061 ctcaatagcg aagcttatgc tcctttgcga acagatagta ttcctatagg ggtaaaagaa
    27121 aatgctgcag gaagtgcttt cgattttcaa atacctaaaa aattagcgtc cgtttttgct
    27181 agtgatttgg atcaaaaaaa tcttgttgac ggaatcgatc atccattttt cttaaaagaa
    27241 accggcttgg gtaaagaagc cgctcgcttg actagccccg ataaaagaat ccaagtagat
    27301 attgctacag atgcttctag tgtggttatt ttcactgcaa atttcggcac agaaacacca
    27361 gaaatgcgca accgaaaact ggcacatcac ggtggcatca catttgagac tcaaacagct
    27421 ccaggtgcag agcgcttctc agcatttggt tcgattcatt taaaagccgg atcagtattt
    27481 gaaacagtaa cagaattcaa aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa
    27541 agaagcttaa acacaaaatt tgccgaaaca ttcggaccta aaaaaactac acaatacttc
    27601 tcaccaggac gcatcaattt gattggtgaa cacacagatt acaatggagg ccatgtcttc
    27661 cccgcttcca tcacttatgg aacgcaaggt gtagcagcac ctcgagacga taataaaata
    27721 ttagtttatt ccacaaactt tgacgatatc ggtgtcatta gttttacttt agatgagcta
    27781 gaatatgata aaaaagcaaa ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa
    27841 gaagctggac actcaatcga tcatgggttt gagttattag tggaaggaac aatccctaat
    27901 ggtgcaggac tttccagtag cgcttcatta gaattattag taggtgttgt attagaagat
    27961 ctgtttgatt taaaaacaga tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat
    28021 gaattcatcg gtgtcaattc aggtatcatg gatcagtttg ccatcggctt cggtgagcta
    28081 gatcatgcaa tcttattaga tacaaatacg ctgaaatacg aaatggttcc agtgaaacta
    28141 gatggttatg caatcgtcat catgaatacg aataagcggc gggaattggc agattcaaaa
    28201 tacaatgaac gccgtagcga atgcgaagaa gccttggcac gtttgcaaaa aactttgcct
    28261 atctcttctt taggagactt agatgaagaa gaatttgaat caactattga ccaaatcggc
    28321 gatgatacat tgatacgcag agctcgccat gccgtttctg aaaatcagcg gactttgaag
    28381 gcaaaagctg aattagaagc tgggaactta gaagcatttg gtcaactgtt aaatgattct
    28441 caccactctc ttcgctatga ttatgaagta actgggatcg aattagatac actagtagat
    28501 gctgctcaaa aacaagaagg tgtcttaggt gcacgaatga caggagctgg atttggtggc
    28561 tgtgcgattg ctcttgtcaa agaagaaaat atacctgcct tcaaaaacaa tgtctatgat
    28621 gaatacatga aagttgtcgg ctatgctccg gaattttatg ttgcacacat tggaaatggg
    28681 acaacaaaga tcgatccaga agctgttcat gtttgataaa aaaatcaacg atctcagcta
    28741 aaaacaaata gataaaaata acctctcagc aatttccaaa tgtgagcctt ttgctcgtat
    28801 ttgaaaattg ctgtttttta ttgacacgga aagagaacgt gtgttctaat tgtgttgaag
    28861 aggtgattgt tatgtgggcg aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa
    28921 gagatatttt atgtattgac tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg
    28981 atcttgatcc ccttacaact aaattagtag tcatgtccta tccgtccgat tcgacagaaa
    29041 cacggggtag cggattaatc ttggcttcca gtcctgcagc aaaaaaagct tacggcatca
    29101 ccaatatcag ccgtgccaga gatcttccct tcccctatcc aagtgattta tatattgttg
    29161 ccccaaggat ggcctattat atggaaaaga acagagaaat caattctatc tataaaaaat
    29221 atgcagacga agcgaaccat catgtctatt ccgtcgatga aagcttcctt gatgtaacaa
    29281 atagcatggc actctttggt aaaacaaatg cgtacgacat ggctaaactg atccataaag
    29341 atgtgtttga gcagaccgga atctacacga ctataggaat tggcgataac ccgctacttg
    29401 ctaaactagc cttagacaat ggctcaaaag acggaaagca aatgtttgct gaatggcgtt
    29461 atgaagatgt accagaaaag ttatggaaaa tctcagatat cactgacttt tggggaatcg
    29521 gcaaacgaac agcaaaacgt ctgaaacaaa tgggtataca ttctgtttat gatttagctc
    29581 acgcggatta ttatcacttg aaaagcaagc taggggtcat cggtactcag ctttatgccc
    29641 atgcttgggg catcgatcga agttttttgg gacaagaata tacaccaaaa tcaaaaagta
    29701 tcggaaatag tcaaatccta gatagagatt atacaagacg agaagaaatc gagatcgtta
    29761 tcaaagaaat ggccgaccaa gtcggcactc gcttgagacg atctgcagca aaaacacaag
    29821 tagtcagctt atgggtagga tactctttag gctatcttga tccaaatggc aagaccgggt
    29881 ttcaccagca aatgaaaatc gaagcgacaa atgctagcag agatctagca gatgctttat
    29941 tgaccatttt ccaccagcat taccactacc aggatattcg gcgtgtaggc gtgaactgtt
    30001 ccgacctcgt atacgcgaca ggtttacagc ttaacctttt tgaagatcct gaaaagcaga
    30061 taaatgaagc gaaagttgat tttgttgtcg atacgattag aaaaaaatac ggctttcaat
    30121 caatcatcca tgcgcatagt ctcttgcaag gaggacgcgc catcgcacgt agttctctcg
    30181 ttggcgggca tgcaggtggt atgagtggat tagaaggtgg aaaagatgag acggacagca
    30241 aaaaaattca ttgactataa cgagtatcga gatcggccat ttggattaaa atgggggact
    30301 gcttttgcga tggaagaact agtcaacggt atcaaaaaaa tgaagaagaa gcacgcaaac
    30361 agcctcttcc taaacaacaa atgacattgc aagaaatcga tagtatactg acagaagcct
    30421 ttttatctcg taaagcagta atcattcagc tcaatttaaa agatgaattt ggaagactct
    30481 tggatgaggt cgaagggcac ttcattggtg aagcctatga agattacttt atgattgacc
    30541 atcagccgat attgtgggaa gacgtacggt atattgagtt aaaggaaaca aaaaaatggt
    30601 ttgtgacatg agaggttgtg acagaagtga tcaacttcaa aaaataagaa aggatttttc
    30661 gaaaattgct tcttaaattt ttgtggaatt ttggcttatt tttcgaagaa gcaacttctg
    30721 gaacaacgta tattagatat cagaggttgt gaaaaagtcg cttagttttg agtaacaagc
    30781 caaggcgctg atttcacaac ctctgtctat ctatccatct attcggtaag attcatgcat
    30841 gacgtcttct ttatttagta tgtagagccc tttttcttct gaccattcaa cataaatcag
    30901 ctgttttaca tctgcatagc cttctttctt caagttctcc ataagccatg cttcagtcaa
    30961 ttgattggac tgaagcactt tatgctgaat ttgcccttca tctacaagca ggatcgataa
    31021 agctttgtct tcatctgatt ttttcaatac actcatttga ccggcattct ctaggataac
    31081 atgtttggca ttttcaagaa aaaagcattc ttgctgtctt aagattgctc gcaactgctc
    31141 catctcgata tggttatttg tcaactctgt catgttgatg acgccgtctt tgattagaac
    31201 agatggctcg cctttgaccc aacgattgac cttttcattt ttttggacga tacgttcgat
    31261 accataaatc attactgccc aaagcgcaat ggcaaatagg acatgcccta catggacatt
    31321 gtcatcatag gttgattctt ctaagatccc acctaaaaca agcgtataga ctaaatcaaa
    31381 cggtgtgatt tctgacatgg atttcttccc aagcaaacgt accactaata ataaaccgat
    31441 caacccgaac accagtttca ttgtaattga aaaaatcatt ttttctcccc ctaactatct
    31501 gttaattaaa ttatatatgc tttctcacga acacgcatgg aatatacaag gaagaaatgt
    31561 atccttttac taaaaaaagg actgtgacac tgtagtgacc cccaaaagtt agactttttt
    31621 ggagtggaga aatccactcc ttttttttat gctctaaaat gatattttta cacaaccgtt
    31681 acggttgttt tacgaaattg gacg
//
LOCUS       DAZKPO010000027        31701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000027 DAZKPO010000000
VERSION     DAZKPO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.027"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8NZ_001198"
     CDS             complement(<1..563)
                     /locus_tag="ACG8NZ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0538891.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8NZ_001199"
     CDS             complement(750..1400)
                     /locus_tag="ACG8NZ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538892.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8NZ_001200"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8NZ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0538893.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8NZ_001201"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8NZ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0538894.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8NZ_001202"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8NZ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0538895.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8NZ_001203"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8NZ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0538896.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8NZ_001204"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8NZ_001204"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0538897.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8NZ_001205"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8NZ_001205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0538898.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8NZ_001206"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8NZ_001206"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0538899.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8NZ_001207"
     CDS             12268..13770
                     /locus_tag="ACG8NZ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0538900.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8NZ_001208"
     CDS             13772..14509
                     /locus_tag="ACG8NZ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538901.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG8NZ_001209"
     CDS             complement(14680..15873)
                     /locus_tag="ACG8NZ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0538902.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG8NZ_001210"
     CDS             complement(15952..16158)
                     /locus_tag="ACG8NZ_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0538903.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG8NZ_001211"
     CDS             complement(16151..17293)
                     /locus_tag="ACG8NZ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0538904.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG8NZ_001212"
     CDS             17805..18440
                     /locus_tag="ACG8NZ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0538905.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG8NZ_001213"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG8NZ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG8NZ_001214"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG8NZ_001214"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8NZ_001215"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8NZ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0538906.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACG8NZ_001216"
     CDS             21086..21253
                     /locus_tag="ACG8NZ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0538907.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACG8NZ_001217"
     CDS             complement(21313..21666)
                     /locus_tag="ACG8NZ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538908.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="ACG8NZ_001218"
     CDS             22170..22589
                     /locus_tag="ACG8NZ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0538909.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="ACG8NZ_001219"
     CDS             22615..22821
                     /locus_tag="ACG8NZ_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538910.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="ACG8NZ_001220"
     CDS             complement(23049..23222)
                     /locus_tag="ACG8NZ_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538911.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23234..23863
                     /locus_tag="ACG8NZ_001221"
     CDS             23234..23863
                     /locus_tag="ACG8NZ_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0538912.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG8NZ_001222"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG8NZ_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0538913.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG8NZ_001223"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG8NZ_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0538914.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG8NZ_001224"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG8NZ_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0538915.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG8NZ_001225"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG8NZ_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0538916.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG8NZ_001226"
                     /pseudo
     CDS             complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG8NZ_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(30257..30484)
                     /locus_tag="ACG8NZ_001227"
     CDS             complement(30257..30484)
                     /locus_tag="ACG8NZ_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0538917.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31575
                     /locus_tag="ACG8NZ_001228"
                     /pseudo
     CDS             30968..31575
                     /locus_tag="ACG8NZ_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT         9255 a         6465 c         5594 g        10387 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat t
//
LOCUS       DAZKPO010000028        31594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000028 DAZKPO010000000
VERSION     DAZKPO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.028"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..507)
                     /gene="dtd"
                     /locus_tag="ACG8NZ_001229"
     CDS             complement(61..507)
                     /gene="dtd"
                     /locus_tag="ACG8NZ_001229"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0538918.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(518..2731)
                     /locus_tag="ACG8NZ_001230"
     CDS             complement(518..2731)
                     /locus_tag="ACG8NZ_001230"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0538919.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(2964..3728)
                     /locus_tag="ACG8NZ_001231"
     CDS             complement(2964..3728)
                     /locus_tag="ACG8NZ_001231"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0538920.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3729..4676)
                     /gene="prmA"
                     /locus_tag="ACG8NZ_001232"
     CDS             complement(3729..4676)
                     /gene="prmA"
                     /locus_tag="ACG8NZ_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0538921.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4689..5177)
                     /locus_tag="ACG8NZ_001233"
     CDS             complement(4689..5177)
                     /locus_tag="ACG8NZ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0538922.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5143..5814)
                     /locus_tag="ACG8NZ_001234"
     CDS             complement(5143..5814)
                     /locus_tag="ACG8NZ_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0538923.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5952..7238
                     /locus_tag="ACG8NZ_001235"
     CDS             5952..7238
                     /locus_tag="ACG8NZ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0538924.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7310..7504
                     /gene="ssrS"
                     /locus_tag="ACG8NZ_001236"
     ncRNA           7310..7504
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8NZ_001236"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7582..7866
                     /locus_tag="ACG8NZ_001237"
     CDS             7582..7866
                     /locus_tag="ACG8NZ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538925.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7939..8418
                     /locus_tag="ACG8NZ_001238"
     CDS             7939..8418
                     /locus_tag="ACG8NZ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0538926.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8641..8784
                     /locus_tag="ACG8NZ_001239"
     CDS             8641..8784
                     /locus_tag="ACG8NZ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0538927.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9129..9257
                     /locus_tag="ACG8NZ_001240"
     CDS             9129..9257
                     /locus_tag="ACG8NZ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538928.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            9362..10945
                     /gene="abc-f"
                     /locus_tag="ACG8NZ_001241"
     CDS             9362..10945
                     /gene="abc-f"
                     /locus_tag="ACG8NZ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0538929.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11382..12566)
                     /locus_tag="ACG8NZ_001242"
     CDS             complement(11382..12566)
                     /locus_tag="ACG8NZ_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0538930.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12591..13598)
                     /locus_tag="ACG8NZ_001243"
     CDS             complement(12591..13598)
                     /locus_tag="ACG8NZ_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0538931.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13770..14129)
                     /gene="comGG"
                     /locus_tag="ACG8NZ_001244"
     CDS             complement(13770..14129)
                     /gene="comGG"
                     /locus_tag="ACG8NZ_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0538932.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14101..14526)
                     /gene="comGF"
                     /locus_tag="ACG8NZ_001245"
     CDS             complement(14101..14526)
                     /gene="comGF"
                     /locus_tag="ACG8NZ_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0538933.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14489..14836)
                     /locus_tag="ACG8NZ_001246"
     CDS             complement(14489..14836)
                     /locus_tag="ACG8NZ_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538934.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(14808..15278)
                     /gene="comGD"
                     /locus_tag="ACG8NZ_001247"
     CDS             complement(14808..15278)
                     /gene="comGD"
                     /locus_tag="ACG8NZ_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0538935.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15253..15531)
                     /gene="comGC"
                     /locus_tag="ACG8NZ_001248"
     CDS             complement(15253..15531)
                     /gene="comGC"
                     /locus_tag="ACG8NZ_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0538936.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15528..16577)
                     /gene="comGB"
                     /locus_tag="ACG8NZ_001249"
     CDS             complement(15528..16577)
                     /gene="comGB"
                     /locus_tag="ACG8NZ_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0538937.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16534..17463)
                     /gene="comGA"
                     /locus_tag="ACG8NZ_001250"
     CDS             complement(16534..17463)
                     /gene="comGA"
                     /locus_tag="ACG8NZ_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0538938.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17595..19349
                     /gene="ade"
                     /locus_tag="ACG8NZ_001251"
     CDS             17595..19349
                     /gene="ade"
                     /locus_tag="ACG8NZ_001251"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0538939.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19447..20109)
                     /gene="trmB"
                     /locus_tag="ACG8NZ_001252"
     CDS             complement(19447..20109)
                     /gene="trmB"
                     /locus_tag="ACG8NZ_001252"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0538940.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20179..20961)
                     /locus_tag="ACG8NZ_001253"
     CDS             complement(20179..20961)
                     /locus_tag="ACG8NZ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0538941.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21165..22376)
                     /locus_tag="ACG8NZ_001254"
     CDS             complement(21165..22376)
                     /locus_tag="ACG8NZ_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538942.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22369..23106)
                     /locus_tag="ACG8NZ_001255"
     CDS             complement(22369..23106)
                     /locus_tag="ACG8NZ_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538943.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23244..23678
                     /locus_tag="ACG8NZ_001256"
     CDS             23244..23678
                     /locus_tag="ACG8NZ_001256"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0538944.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23671..24015
                     /locus_tag="ACG8NZ_001257"
     CDS             23671..24015
                     /locus_tag="ACG8NZ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0538945.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            24195..25205
                     /locus_tag="ACG8NZ_001258"
     CDS             24195..25205
                     /locus_tag="ACG8NZ_001258"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0538946.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(25274..26218)
                     /locus_tag="ACG8NZ_001259"
     CDS             complement(25274..26218)
                     /locus_tag="ACG8NZ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0538947.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(26202..28922)
                     /locus_tag="ACG8NZ_001260"
     CDS             complement(26202..28922)
                     /locus_tag="ACG8NZ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0538948.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(28919..30139)
                     /locus_tag="ACG8NZ_001261"
     CDS             complement(28919..30139)
                     /locus_tag="ACG8NZ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0538949.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(30420..31265)
                     /locus_tag="ACG8NZ_001262"
     CDS             complement(30420..31265)
                     /locus_tag="ACG8NZ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0538950.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         9413 a         6484 c         5479 g        10218 t
ORIGIN      
        1 tttatcagtc taacttaagc gacgtgtttc atagttttta ctagtgaaaa ccagattact
       61 ttatttgttt ttagaatcaa taatgatcgt tacaggtcca tcattgacga gagaaaccga
      121 catatctgct ccgaattgtc cggtagcgac tgtgatgccg cgacttctga ttccttcatt
      181 gaatgattcg tataaaggaa tagctgtttc tggtcgtgcg gcttccacaa agcttggtcg
      241 atttcctttt ttcgtatccg caaacagtgt gaattgagag atacttaaaa tttgtcctcc
      301 cacatcttct acactaatat tcatttttcc ttgttcatct tcaaaaatac gcatttgtgc
      361 aatttttttt accagatagt ccacatcttt ttgcgtatcg gtttcatgaa tgcctaataa
      421 aacaagtagg ccgtagccaa tttttccaat ttcttgctga tcgactgaca cagcagcttt
      481 acttacccgt tgaacaactg ctctcatatc tgtcctccta tccattcgtc cggcgtacac
      541 tgtagacatc aggtactgct ttgattttat ccacgatact ctttaggtgg gataaattct
      601 ggataccaac cgtcaaatgg atcgtcgcca ttttatcttt attcgtccgt gcttcaacgc
      661 tgttgagatt tttcgttagc gcattgactg tttgaagtac atcattcaat aagcctgaac
      721 gattatagcc gtaaatttcc aaatctgcat catactcttt tcgtgtattc gatgtatctt
      781 cccattcgac ttcaatcaaa cgttctgcta catttggttt gtcaggctga acattcggac
      841 aatctcgacg atgaatggat atccctctgc ctttagtgat ataaccaacg atatcatcac
      901 caggaatcgg attacagcag cgactgatac gaattagtaa gttttctacc ccttgaatga
      961 caacgccacc ttcatgacgt actttcattt tgtcaggttc ttttttcggc tgattcatga
     1021 tttcttctgc ttcttgcttt tgctgctcga ttttttgttc tttacgttct ttttcagtca
     1081 gacgattggc catcgtcaag gggctaactt ctccataccc tacagctgca tagagatcat
     1141 cttctgtttg ataattaaaa cgatcgagtg cttcttgcag tttgttcttc gtcaaaatat
     1201 ctttaggcgt aaatcctaga tctgtaatac atttgaccac ggattcgtgg cctttgatca
     1261 cattttcttc tcgatcttgg gctttgaaga aacgtttgat cttatttctt gctttgctag
     1321 tagcaactaa tttcaaccag tcgcgacttg ggccaaatga atttggagaa gtcatgatct
     1381 caatgatatc tccgtttttc aatttgtaat caagctgcac cattttgcca tttacttttg
     1441 caccagtggt tttgttaccg atatctgtat ggatactgta tgcaaagtcc aatggtccgg
     1501 atccttttgg taactcagta acgtctcctt ttggtgtgaa gacgtagact ttatcactaa
     1561 agatatctcc ttttacgcct tccataaatt cagaagcatc atagctttcg tcttgaagtt
     1621 cgagtatctc atggaaccag cttaattgtt tcgtcatacc atctggttct actttttcat
     1681 tttttccttc tttataggcc cagtgtgcag caaccccgaa ttcagcgatt tcatgcattt
     1741 cctgtgttcg gatttgaatt tctaccggat ttcctgccgg accgatcacg gtagtatgca
     1801 aagattggta catattagct tttggcatgg caatataatc cttaaatctg ccgggcattg
     1861 gtttccattt tgtgtggatt gctcctaata cagcataaca atccttgatt gaatctacga
     1921 tcactcgaat agctagcaag tcataaattt cgttgaactg ctttttctga tctttcattt
     1981 tacgatagat tgaataaatg tgttttggcc gtccatagat ttctgcaaaa atccccagct
     2041 cttctgtcgc tattcgaata tcttcaacag taccacttac gtatttttca cgttcttctc
     2101 tcttcgtctg catcaagtgg acgatacggt aatactgttt tggatttaga taacgaagtg
     2161 ctgtatcttc taattcccat ttgatccggc tgatccctag acgatgtgca agaggcgcat
     2221 agatttctaa agtttcctga gcgatccttc tttgcttatc ttctcgcagg tgcttcaacg
     2281 tacgcatgtt atgtagtcta tcagctaatt tgaccatgat aactcgtaaa tcttgagcca
     2341 ttgctagcag catctttcgg tggttttctg ctagctgctc ttcgtgggat ttatatttta
     2401 ttttccctaa tttggttacg ccgtccacta acatagcaac gtcatcgcca aattcttctc
     2461 tcagatcttc taaagtgatt tctgtatctt caacaacatc atgtaagaag cctgtagcca
     2521 ctgtatgggg atccattttt aaatctgcta atatacctgc gacttggata ggatgaataa
     2581 tgtagggttc gcctgatttt ctaaactgtc cgtcatgtgc agcagtcgcg tattcgcatg
     2641 ctttctcaac aaatgctaca tgttcgggac ccatgtattg actaacgagc tttatgacac
     2701 cgggtcccgt taaaatttct tcttttgtca caggaatcga tccccctttc ttttttcggt
     2761 aaaacgggaa tcgggacaaa tatctgtttg ataattgtcc caaaatccgt gcagttatac
     2821 gtttttttac ggaaatgttc gctttttaaa agctttttca aacgattttt cgtatccatt
     2881 gtataaataa ttgtttctat tatacaaatt gttgactatt ttttcaattc tttctgtcga
     2941 tactctttta ttaatgaaga acgttagctg aaagaaggat tcattaattc ccattgatag
     3001 ctagcagcac ttaataaata aagaggtgct gtttctgtac gaaggattct tggtcctaat
     3061 ccacaaagta tccctttgtg ttctacaaaa taatcgactt catcgcggga taatccacct
     3121 tctggtccaa agacagctaa gattcgacta tctggttttg tttcactaag aattttggct
     3181 gcatttgatt tttcgccttg ctttgctgat tcctcataag caaccaagac cacgtcataa
     3241 tcatctagcc gctcaaataa atcttgtttc gtttccaata actctactga tggcacgatc
     3301 tgccgatggg attgttccgc tgcctcttga gcaatctttt caagccgttg ttgtcgtttc
     3361 atacgttttt ttgcatccca tttaacgaca gaggctgatg ccgggaaacc aagaaaagct
     3421 tgcgctccta attcagtgga tttctgaacg atccaatcca gcttttcccc tttaggatag
     3481 ccactcgcta tggtaatcga acttggcagt tccttttgat tttcttcttt tttaatttct
     3541 ttcagataaa cagcctcttc agtaatctcc gtgatttctg caacaatggc tatttgatca
     3601 ttgaatgcca aaaaaacttc atcctcgggt tccatcctca taacccgtac aatgtggtga
     3661 tagttttccc cttctaaaac aaaatagtct tttgattcat atgattcttt taaaaaatac
     3721 cgctgcatct attccacctc tggttttttc aaaataattg cataccagtc tttttgctgg
     3781 aaaatttgat ccacgataaa cccttgttct aacatggcat ccagaaccat ttgcttcttc
     3841 tcttcaatga ttcctgaaac gatcagcgtg cctgtttgct tcagtaaacg ccaagcatct
     3901 gggatcatca ataagatgat atctgctaaa atgttcgcaa cgattacatc actttcaatc
     3961 tcgattcctg tcaaaagatc gttagctgat acatggacat ctttggctat ttcattcata
     4021 tccatatttt ctttcgccga acgaacggct acttcgtcta aatcaaaggc atagacatct
     4081 tttgcaccaa gatattttgc tgcaatactt agcacgcccg acccagttcc cacatctagc
     4141 aatgtttcac caccacgcaa gactgtttct agtgctgtca atgtcaagga agtcgtggga
     4201 tgagtgcctg tgccaaatgc cataccagga tctagtgtga taattttttc tagaggatct
     4261 tctgtctggt agttttccca gcttggcgca accgtcagca ttcgcgtaat acgcaccgga
     4321 tgatagtatt tcttccaggc tgttgcccag ttgctttcct ctacttcact aactgttact
     4381 ttattttttc caatagctag accaaactca ggtaattgtg tgatacgatc tttgataaaa
     4441 ggcaagattt ccggtaagaa agtcgtttcc ggaaaataag ccatgactag tgcgccttct
     4501 ttcagcgatg taaaatcatt tttatctaaa atctcaccat attgatccga ctgaaaatgt
     4561 tcaacatcta aagaatcttc aatcgcgaca ccactcgccc ctgcctccat caaaatatta
     4621 gcaactgctt cgactgcttc actttcagtt tctactttta cttcattcca tttcatatct
     4681 gtcactcctt aataacttgg gtacggtaaa taagtatctg cttcattttg ctgttttact
     4741 tctactaatt ctgctagttg ctgattatcc cagtgaagct cgaatacagc ttcgtcatcg
     4801 tcttcatcca aaaaatcaag caaatcgctt tgcccttgag ctaatacttc atttagatat
     4861 tccactaaag aatcagctac tgattttgcc aacccttttt ttccttcata agggattact
     4921 gctagataat tgcgttcatc aaaaacagat ttctgaggat taaaaagaag tacgccgtct
     4981 tcaaattcga taattggttc ttgcgaaacg atcccttctg catcatcaat ctcaaattgt
     5041 gctttatttt ctgcaaacaa gcgaacaacg atctcgatcg tatgattttt tgtatcccaa
     5101 tctaaagcta catcatagtc tgtcacttgt ttttcaatta tttcatctaa atatgttaat
     5161 agatccttct ttttcatttt ctttcctcca accccaatct ttctcgattt ggttttttct
     5221 ttgtaaagag atataaggat ttcccgacac aacaaaacgc aacggctttt ctgtccatac
     5281 acctttgttt gggataccga ttctaggcaa agcattgatc ttttttggtg tctttttttt
     5341 ctcaaaaact aagcgtaacg aactatctgc tatagactga ccataaagtt cttgaggaat
     5401 tgctaatgct tccactaatt ttcccggacc gttgctgata tctggaccag tttttccgcc
     5461 acgattttta ctcatttgat caatcattgc ggcgggttcg attgcacgta tcattactcc
     5521 ttgaggaatc ccttctggct gagtaatgat gttcaaaatc cgatgagtat gcatcgtgta
     5581 aaggtagatc gtacctggtt tttcatacat tgcccgcaca cgcggtgttc ttcttaaacc
     5641 atagctatgg gcggcctcgt cttctggacc taggtatgct tccgcgtcta caatatatcc
     5701 agctaatctt ccttctggtg tgtcatgctc caaatacatc cccaataaat cccgtgccac
     5761 ttctggcgtc gttttattcc aaaaaaattc atctatcttt tgtttatccg tcatttttct
     5821 cacctacttt atcatacaca atattagttg gataagctat gaaaacaaaa caacagaagg
     5881 ctgttctttc taggcatctt gctaaatatg atataattaa gggacgtaaa aacaagaagg
     5941 aggaaaatcc tatgcaacag ccattggctt atcgcatgag acctcgaacg atcgatgaag
     6001 tcgttggtca gcagcatcta gtcggggaag gaaaaatcat acgccgcatg gtcgatgctc
     6061 gcatgctctc ttccatgatt ctatatggac ctcctggtac tggaaaaacg agtatcgcaa
     6121 gtgcgatcgc cggctctact aattatgcat ttcgcatgct aaacgccgct acagattcaa
     6181 aaaaggatct acaagttgta gcggaagaag caaaaatgag tggtacagtg atcctcttat
     6241 tggatgaagt acaccgatta gataaaacaa aacaggattt tctgcttcct catttagaaa
     6301 gtggccgaat catcttgatc ggagcgacta ctgagaatcc ttatattacc atcaatccag
     6361 cgatccgcag ccgtacacag atctttgaag tcaaaccatt aacagaacaa gacatccagt
     6421 tggcagtcga acatgcttta agggataaag aacgcggcct tggacaacaa gcaatccagt
     6481 tagacgaaga tgctcttctt cacctttcgc gtgcaacgaa cggcgatttg cggagtgcat
     6541 tgaatgggct ggaactggct acactatcga ctccttcaga taaagaagga aggattcatc
     6601 ttacactgtc gatcattgaa gaatgtgtcc aaagaaaagc tcttacccat gacaaaaatg
     6661 gagatgctca ttatgacgtt atttcggctt ttcaaaaatc gattcgtgga agtgatgtgg
     6721 atgctgcact tcattatctc gcccgtttag ttgaggcagg tgatctggca agtatttgcc
     6781 ggcgtctgat ggttatcggt tatgaagata taggtttagg taatcctgct gctgcggctc
     6841 gtacagtgaa cgctgtatta gctgctgaaa aattgggatt gcctgaggca cgtatcccac
     6901 tagcagacgt cgtcgtcgat ctttgcttgt cgccaaaatc caattctgcc tacatggcat
     6961 tagatgcagc acttgctgat attcgagagg gcaaagctgg agatgttcct gatcatttga
     7021 gagatagcca ttacaaaggt gcgaaatcac tcaaccgtgg cgtgggttat cagtatcctc
     7081 atcattttga tcaggcttgg gtcaatcagc aatatttacc agataaactg aagaatgctc
     7141 agtattatca gccaaaagat acagggaaat atgaacaggc tctgggtcaa cagtattacc
     7201 gtataaaaga gtggaaaaag catcctccaa aaaactagac aagaaaacgg ttctgtttct
     7261 gcgagctctc tcattgctct ttttaaattc atatgctact atagaggtgg gaaatctgtg
     7321 atgttcgaat tgtcctcttt gtgtgttgac cgaacatttt tattgatatc ttgggacccg
     7381 tcttgtgtca ctgctggtaa catgccttat catttggtag ttcgaagcta cgaccatggg
     7441 acccacctgc ttgaattcgc gggatcaata ctacaggaca aacaacggca ttaacggaga
     7501 ttaccatctt ttaaagaata aagcaatttg ctcttggcaa attgcttttt ctatttgttg
     7561 cttattttgg aggatttgtt catgattagg attttaatga ttatcgccac tttagtatta
     7621 ttattcgtta gctattattt atttaataaa caggatatct tttttgtatt gatcaacaaa
     7681 aatgataaaa atcaaggatt cctacaattt tacggagctg catatgctgt tttaggcgta
     7741 atgggtattt taacagcttt tttcaatcaa cgctttattg ccttggtctt tttactgatc
     7801 gtaattattg tttctgccac cttcagtatc cgatttgcaa aaaaaattgc tgaaccaaag
     7861 cagtaaataa ttctcttttt ccctaaagtg ttctacaata aaagagtaca atacgattga
     7921 cttgaggagt gaatgcttat gttacaacaa tacaaaaaaa tcatggttgc cgtagatggt
     7981 tctgatgaag ctgaattagc ttttaaaaag gcggtcaatg tagctatccg aaataacgga
     8041 gaacttctat tagcacatgt cattgacaca cgttctttcc aaaccgtttc atcctttgat
     8101 ggaatgcttg cagaacaagc tactgaaatg gcaaagcaaa cattggcaga ttatgaaagc
     8161 aatgcgaaga aagctggctt gaataatgta acctctgtcg tagaatatgg ctcaccaaaa
     8221 caaattattg ccagagaaat cccagaagat aatcaagtag atttgattat gttaggagcc
     8281 acaggattga atgctgtaga acgtttattt attggttctg tttctgaata tgttattaga
     8341 aacgcagctt gcgatgtgct tgttgtccga actgatttag aaaaccaatt gccagctaaa
     8401 gaccacacca aagcttaaca cgtagacgac ctgccaacta tcggcaggtt tttttgcaca
     8461 aaaaaccgtg ataaaaacaa aagacgcggc aagcactcat cggtctttat aatgtttaaa
     8521 ataaagggaa aacagtcaat gagttcaagt aaaataaaaa aaagtgaaca ccagggcgat
     8581 tagtcgccct ggtgtggttt aaaacgacct aagcatgcca aagcttattg gttcgtcaat
     8641 gtggaagttt ccgatgaatc tgatacgaaa aaatatgaag tatcgatggc ggataccgat
     8701 tttatcgcca aactaaaatt ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca
     8761 ttcggtaaat cattcaagaa ttgatgaaag gctaccgctt gccattgcaa ccacaacgat
     8821 actcctgaac gcgatgttgg tgagatttcc aaagagaaga gctgtttaat gcaacggtat
     8881 taagcaaatg gcaaaaggaa gtcatgtaga caaagttttt tatcaaacac catatgattt
     8941 cgcctgaacg ctggtttagc tcagaataca atgatgtggt ccacgagaca ttaccctcag
     9001 tcagataaaa aaagctctct gacagaatta cacatcgata aatattaagt aaatgtcagc
     9061 gctagttgaa ctttctgaca ctttcaggta cactggaaaa gacaactaca cgaaggaggg
     9121 aaaatactat gatgcgcatg actaaaataa caagtaatga aaattaccaa cgataccata
     9181 tcgcatttag ccatgcaatt ggttttacag ttgctgttga cgggacaagt ttgtccaaaa
     9241 cagttgaatt cacgtaaaat caatggccat ctcaaggtat ttttctcatg ataatctgag
     9301 tttaaataac acaggccatg gataaccatg gcctttttga gtggtctcaa ggagagagat
     9361 catgatcata caagcaattt ctattaaaaa aacaatcggc gggaatccat tatttgagga
     9421 tttatcctgt caaattgaag ctggtgaaaa aattgcctta gtcggtatca acggaactgg
     9481 caaatcaacc tttttacaaa tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag
     9541 tcgcaaaaaa ggcttaaaga tcgggacggt cgaacaagaa cttacagttt ccgaagcgac
     9601 cgtcaaccac tacttgctcc attctgccgc tgagatacag gacctcaaac agcaaatgag
     9661 tcgctacgaa gcattgatga cagaaccaga tacaaactta gagaagtgtc tggcccgtta
     9721 cggtgagctg cagcatcgct ttgaagaact aggcggttac gtattagaag accggattgc
     9781 agcgatcttg caaggcttag ggattccaca ccgtcaggaa gcacggttaa cagaacttag
     9841 cggaggcgaa cgagttaaag tcgctttagc caaaatttta gcagccgatg ccgatttact
     9901 tttactcgat gaaccgacca atcatttaga tcttaacagt attcgctggt tggaaaacta
     9961 tttacagaat acaaaaaaat cggttctggt cgtttctcat gatcggcaat ttctcgatca
    10021 agtcaccaca aaaacatggg aactggaaga aggtgcattg attgagtatc ccggaaacta
    10081 cagccgtttt cgagtcttga aagaagctcg cttggctgag ctgacaaaaa attacgaact
    10141 gcagcaaaaa gaagtccagc ggttgaaagt catgattcgt cgttttcgtc agtgggcaca
    10201 tgaaggagac aatgagtcct tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa
    10261 actgacactc gtcaaaccgc cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg
    10321 tggaaaatct ggcaaagaag ttttcatcat tcaaaacttg caccagcagt acgctgatca
    10381 agtactattc aaagacagca gttttgcagt ctatcgtgga gatcatttag ccatcatcgg
    10441 cgacaatggt gcaggaaaat caaccttact caaattatta ctcggtgagg aaaagcctta
    10501 cggtggcacc atcaaattag gtagtagctt gcaaatcggt tatttgccgc agcagctgca
    10561 gtttgccgat cccgatagcc gtttgctggc ttacgccatc acattgactg gaaacgaaga
    10621 agtcgctcga cggcaattag ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat
    10681 caaagattta tccggtggtg aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa
    10741 aatcaacgtc ctgattttgg atgaaccaac caatcacctc gacagctatg ctcgagaaga
    10801 aattgaagaa atgctgcaag actatcaagg aacgctccta gcagtcagcc atgaccgcta
    10861 ctttttgcag caacacttcc aagaagcact cgttattgat caggagcaga tcagccgcca
    10921 gccgctcggc caactgattg actaggacgt tttttcgcaa caaaaaactt gattttcata
    10981 cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat caagttttta ttattcgatc
    11041 aatgaatgtt tcggatcggt tttctagcta aacataaagc gaagaataaa gtcgacagcc
    11101 cacgaggctg atctaaaaat gttttttctt tttacatatg taatgtccct tataaagatg
    11161 aatgaaaata aaatattggg attttcctga aaatcaataa ataaaatgcc agtcttttcc
    11221 cttttgacca cttttccaag caaaattccc taagaagatc ctgtaaaaaa gaagggaaat
    11281 tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct
    11341 cttgcatgcg tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc
    11401 acatcacggg caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat
    11461 tcttttgtag agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca
    11521 caaccaaacc aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg
    11581 ataccagcag taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg
    11641 tattttcgga tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag
    11701 ttcttttcaa gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc
    11761 ttgttcaaaa cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg
    11821 agaacagctg gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg
    11881 aatcccatag aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt
    11941 cctaagtggc aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca
    12001 cgttccgaca cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct
    12061 tcataataag acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa
    12121 gatgtatcga atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc
    12181 cccattaagt ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc
    12241 attactttct catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg
    12301 tgccctactc cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct
    12361 aacagcattt ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga
    12421 ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct
    12481 ttcgcaacta cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca
    12541 gcattgattg caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca
    12601 gatgatttcc agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat
    12661 gaagcaagtt tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt
    12721 aagatcaaaa tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc
    12781 attccttgaa gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt
    12841 tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt
    12901 gattgctcta gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac
    12961 tcttttgctt tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg
    13021 acaggatcta ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt
    13081 gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt
    13141 ccttctgttt ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc
    13201 attcctgccg caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca
    13261 ataagaaaaa caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa
    13321 tgttctgtct ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc
    13381 tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt
    13441 gtcggcactc catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg
    13501 acataggctt ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg
    13561 ttttgattaa acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat
    13621 attataacaa ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt
    13681 ttcactgttt tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca
    13741 gaaaagaggg cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt
    13801 acttcttctg tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt
    13861 tgtacccact gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg
    13921 gcaagcgtag actgttccga taaagccatc tgcttcatta tttttgctat ataataattt
    13981 ttgattcgta tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa
    14041 aacgtacata aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt
    14101 ctactgatcg ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata
    14161 aaaagaaatt gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg
    14221 ttgatgtccg ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata
    14281 ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct
    14341 cttgagttct tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg
    14401 caaaaattca cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact
    14461 gctcaatatc atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt
    14521 tttcaatact gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat
    14581 tatcgacaat ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat
    14641 acatccgctg cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac
    14701 ttttcttttc tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag
    14761 aagcgatcat aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt
    14821 tttgtatagc gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc
    14881 caataaaatt gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata
    14941 gaagcatggc gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta
    15001 tctggattcg gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt
    15061 tggatctgat tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg
    15121 aaaaattgat agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa
    15181 aggatcgttg taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct
    15241 aaaaccttac cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc
    15301 attcaacaat tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt
    15361 ttgtgtgtct accaactgga tgatcgcttc acgactttct tggtctacat gattcctata
    15421 acgcgacaaa ttagggacga acaacaacac taatacactg atgatcagta agacgattag
    15481 catttcgatc aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc
    15541 tcctctatgc ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc
    15601 gctacgccca aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa
    15661 agctgttttc ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc
    15721 tctccctgta aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc
    15781 tcagccaatg acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc
    15841 agactttctt gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca
    15901 tgaaaaagct ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt
    15961 ccaatcaata attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga
    16021 cgatacctta ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa
    16081 tgaaagactt gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata
    16141 agctgaggca aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat
    16201 agcatcggat atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac
    16261 tgtttggcaa tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc
    16321 tgtaccagtt gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct
    16381 tcctttagct gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt
    16441 aataaataaa gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt
    16501 gcaaactggt tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt
    16561 ttcctgagct tcttcataac tttcctccca tgattttttt actgattctt cctccataaa
    16621 ttgataactc cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat
    16681 gacaccagct aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac
    16741 caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc
    16801 tgctcttacc gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc
    16861 tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt
    16921 gatctgtagc tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac
    16981 ttgcaatttt tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgg
    17041 tccgctgaaa aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg
    17101 agaactaaag caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact
    17161 ctctcgttga aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa
    17221 agaataagta aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa
    17281 atacttaaat cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt
    17341 tcgttttttt ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac
    17401 gtcttgggca tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc
    17461 cattattctc ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat
    17521 gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt atacttataa aaatttatct
    17581 tgggaggcac tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa
    17641 agcctgctga cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca
    17701 tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa
    17761 aaagcgtaca taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc
    17821 atatcgaaag ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag
    17881 ttactactgt agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg
    17941 agtttatgat agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt
    18001 gtgtacctgt aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc
    18061 cttttttaca aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag
    18121 caaataatga aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa
    18181 ttgacgggca tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta
    18241 ttcggacgga tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta
    18301 tgtatttaat ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga
    18361 tcacacctga aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt
    18421 tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc
    18481 cattacaagc cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac
    18541 taggtgcgat tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc
    18601 tgccaattca tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag
    18661 accatttatt tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc
    18721 aaccattaaa tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg
    18781 aaattatccc gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg
    18841 aacgattcat tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc
    18901 atcaaacggg aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga
    18961 tagcgacaac gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg
    19021 atatgctgta tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca
    19081 aacagcagga aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt
    19141 tttcgatcgt ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag
    19201 ctaaaacctt tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat
    19261 tgaagatcac cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg
    19321 gaaaaaccga tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc
    19381 tgtaatgttc attttctggg atttatttat ggatattaat gtttttatga aagcagaaag
    19441 ttagtttcac agacttttct ttttggtctt atcttgaaat tgagcttcta ctcgataaat
    19501 acgttgacct tttgaagaaa atttttcttc atactccgtc ataatattgc cttcaaattg
    19561 actttgatgt aaatctaacc atacctgttt gaggatcatg ccatattgag aaaaacttgc
    19621 cagagaatac tcaaacaatc cttgattatc tgttttgaaa tggatttctc catttggacg
    19681 aagaatctgt tcatctacag caagaaaagt cttatatgtc aatcgccgtt tttcatgacg
    19741 tttttttggc catggatcgg aaaaattcag atagatttga tctacttcgc tgtcagcgaa
    19801 atactctgtc aacgctgatc catccacatg tagaagtttc aggttaggaa gcggttgttc
    19861 gatcaacttg tctagtgcaa tcgaaacaac actgacctgc atttctactc caatataatt
    19921 gatttcagga tgggcttttg ccattccagt gatgaactgg cctttcccca taccaatctc
    19981 aatgtggatt ggatgatcgt tttcaaacaa ttcattccat tttcccttcc acaatgttgg
    20041 atcactaatt acaaaattcg ggtgagccgc taacatttcc gctgctcccg gacgatttcg
    20101 gacacgcatt atttatctcc attcctttcc tctaattaaa aaggtctggc caaatctccg
    20161 cagagtttgg gccaaacctc agccactgaa aattcgtttt aattgtaaaa tttcttcgtt
    20221 catccgtcga tgatcttcac gtttgcaata tctggtgatt tcttggagca aattcatggc
    20281 cgcataccaa tgaaattttt caagcatttc gtcagtcggg cgtatgccat aactgatcag
    20341 ccactgattc cagctagaca gtggcacata atttcctaaa atcgttgcaa tatccaccgc
    20401 agggtctgca aacatgattg aatcccaatc gactagatat aaataatttt tacatactaa
    20461 ccaattacga tggtttacat ctccgtgtac tactgataag ttttccttat ctattgacgg
    20521 gatctgctca cgaagaaaac gatggacaat ttgcagaaat tgatttttct ggagttcttc
    20581 cggtaattct ttttcatatt tttgcagcat cttctccggc gtcatgacac gaccaccgat
    20641 acgggtcagc atggttttaa gagaatttga atgatgtaaa tggtacaaaa catcaatcac
    20701 gtcgtttctt tgaccaatct cattaggagt cagcaagcga ccttccagcc actcttgtgc
    20761 tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca agttttggtg taattccttc
    20821 ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta ataaagactt tgtcctcatc
    20881 acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg
    20941 gtctaactga aactccatca tgcattctcc cgtcctctta ctctttgttt tgcagtactt
    21001 attattctag tcgcctgaac actacacgtc aagcacatat acgttctttt tcatttaatt
    21061 gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa
    21121 cgctctattt ttcattctca tttttaccct acacagcttt gttttcagtc tgacatcttc
    21181 ttcaaacgag ttggtacata tagtttaaca aagagcatag taacaatcaa ataagccgct
    21241 agcggaaccc atcgctgctg cccatctaat acgactgcag caatcaaacc aaaaataacc
    21301 gacgcgataa ttaataccca tgtgagtaga tactgaatcg cctgcttctt ctgattctta
    21361 tggattggat aaagctgaac gagaaccata tagcggaact ggttcgctaa aggtaacagt
    21421 tggaaaccga tcaaatataa aaataagaaa ccgataatca gactaaaata aaagtcgact
    21481 actccggcaa tcaataaccc acctataatt actaatcgga aataaagacc actaaaatca
    21541 gaacctcttg ccaatcttct tgcaaacaag taaagataag tgttttttga ttctctttta
    21601 atatgagtca atataggatc caaatagcgt cttcttttga tctttgccgt aatttcagga
    21661 acatctgtga ataaattgat aaattgatag atctgatgca acctatgttc ttctacatgc
    21721 accattttct cccaatccaa tcctttttct tgttgcttcc acaaaaacca gtaatataga
    21781 cttgcctgta ctaatgcacc tgctattcct gcccatatta gccaataaaa tgatagaaac
    21841 aaaaagatca gagaagaaaa aaaccagcct aatttccaat aacgaataac ttgttgatct
    21901 agtcgaaaca attctgctct cttgatttgc agatgactga atttcaaact ccataatatg
    21961 atcagataga cgaaaaacat cgaaaaggat tgctccgtac taaccaccac taatggcatg
    22021 gccaatccgc atgccaatat caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct
    22081 cgttccaaat aggtttgcat ttctttttct ttaggaagta aaaaaacaat atctgctgcc
    22141 tctgcaaaag tagccatctt acccacgaat aaacagagca tccaaaatac agagattatg
    22201 agccctccaa ttgcaaacgg tggctgcagg gttttcaacc aattagaata atataatccc
    22261 acaccgccaa tcaagaaaag acaaactaaa acaaaatgat cgttcaatac ataacgcata
    22321 tatttcatca ttttcttttg atgtcttgct aaacgttttt gaaaaaaact actcatgtgc
    22381 ctgctccttt gttaaagcaa tatagatttc gtccaaagaa gaatccggca gctgaaattc
    22441 ttttctcaac atctcaagag aaccgtctgc tcgtatctct ccgttatgta agaccacaaa
    22501 acgatcacaa tatttttctg cagtagctaa aatatgcgta gacatcaaaa taccggctcc
    22561 ttcttttttc atttctacca tcaattcaag caatgcattg atagctagtg gatccaagcc
    22621 taaaaatggt tcgtcgatga tatataaaga aggctcgatc aaaaaggcgc ataagatcat
    22681 cactttctgt ttcattccct ttgaaaaatt agctgggaac cagtcaagac gattagataa
    22741 ccgaaaagtc tccaacaact tttctgctct tttcattgct atatccatag gtatatcgta
    22801 ggccatcgca gtgacttcga tatgttctct caatgtcaat tcttcatata aagaaggagt
    22861 ctctggaata tacccaattt gtttgcgata ttcttccgta tttttcttta aggatagatg
    22921 attgatttct atcgttcctt ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt
    22981 acttttccct gctccgttca gtccaatcaa ccctactatc tctccaggct gtacatcgaa
    23041 actgatatct tttaacacag ggagatgtcc gtacccccct gtgacatgat cgataattaa
    23101 actcattctt tttcctccgc catttatttt tatttattat accacgaaga aaatgacggt
    23161 attttgtttt tttccttaca atagctttaa cttatggtaa agttaagctg aagtatttat
    23221 tctttataga aaggatgttt tatatggaaa actgtatttt ttgcaaaatc atcaatcaag
    23281 aaatcccaag ctataaaata tatgaagacg acaaggtcta tgctttttta gacatctctc
    23341 aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca tgtagcagat atttttgaat
    23401 atgatcccga acttgcagga gaggtttttt cgcgtgtgcc taagattgct caagcattgg
    23461 aaaaagcttt tccagaaatg aaaggtctta atatcttgaa taacaaccgt gaactagcct
    23521 atcaatctgt ttttcattca catatccatt tagtccctcg ctacagcaaa gaggatgatt
    23581 tttctattca ctttgtcaat catcaagata gttatggatc ggaagaacta aaagctattc
    23641 aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa actttttcaa aggactgtta
    23701 tttggagctg ctgctggcac tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa
    23761 acaagaaaga aactgatcga agaattggaa gaagcaacaa ctctgacaaa tgatttaaat
    23821 aacagcctga atcattttaa aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg
    23881 attcctgctt tccaagaagc aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa
    23941 ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa aagctgatct gccagagtat
    24001 cctgaacaaa tataataatg attattttct caacagattt aactatataa gtgagaaaag
    24061 tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt gtttgaaact accacgacaa
    24121 cgtttttaaa ggaaatggga tatggtatag ttaatatata aaaataataa ctaattacag
    24181 gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc agctactagt gcgttggctg
    24241 ttttaactct agcagcttgt tcaggcgata ctaataaaga tatcgcaacg atgaaaggcg
    24301 gaacgatcac tgtatctgat ttctatgatg aagcaaaact tgaatcatcg aaccaatcct
    24361 tagtacaacg gatgatcatt tataaagtat tcaataacaa atatggcgat aaagtaaccg
    24421 ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc attaggtgat acttttgaat
    24481 cacaattaga agctgctgga tatacgaaag atacttataa agaatatatt cgtaacaatt
    24541 tagcttttga agctggcttg aaagctcacg tagatattac agatgacgat ttgaaaactg
    24601 cttggaaatc tttccatcca gaagtagaag cacaaatcat caaattatca agtgaagatg
    24661 aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga tttctctaaa ttagccaaag
    24721 acaaatcaac tgatacagag acaaaagaag atggcggtaa agttaaattc gactcaacga
    24781 caacaactat tcctgcagaa gtaaaagaag cagctttcaa attgaaagac ggtgagatct
    24841 ccgatgttat tactacaaca aatcctactt cttatgcgac agaatactac gttgtcaaaa
    24901 tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata caaagatcaa ctaaaagata
    24961 tcgctactga aactaaacta agtgacaatg cattcacaac taaagtaatc ggtgaagaat
    25021 taaaagatgc aaatgtaaaa atcaaagatg atgcctttga aaatgtgttg agtgcattca
    25081 caacaacttc atcaagtacg aaagactcaa gtgaaactac agcttcaaca aagagttctg
    25141 acacaaaatc aactgattca acaaaagaat cttcaactga agaaacaact gattcatcaa
    25201 aataaaagaa aacaaaaaaa gcgaagactt atgtctcgct ttttttgttt tgaatgaaaa
    25261 aaataaattt ttgctataaa tcttttggta catagaaact tcggttgtcc ataccgaaaa
    25321 tccgatcggt gtattttcct ggttctgttt gacgaatcga agtcaacatc atctgcatcg
    25381 aagcatcgat attgtcgatt tgatgtaata tttctgcttc cattattctt ggacgtacag
    25441 gtgagccata ttctaataag ccatggtgtg ctaaaaccat atgacgtaat acgagaacat
    25501 cttcgtcagc ttcgctgatt ttcaacgcaa gacaggcttt agttatttct tcgtcaatca
    25561 ataccagatg accgatcaaa tttcctgcaa gcgtatattc tgtcgacata ggaccggaaa
    25621 gttcaatcac ctttcctaaa tcatgcaaaa tgataccagc atacaacaat gctcgattca
    25681 gctcaggata ttcatcggcg attgatttcc ctaagtgaag cattgtcaaa gtatgataag
    25741 ctaatccact ggcaaatgca tgatggtttc tttttgctgc cggaaactca aaaaattgtt
    25801 tttgatattt attaagtaga tagcgaacaa tacgattcca atgcgcattg gtgatctcaa
    25861 acaacaattg attgatttct tcttccattt cttctttctt caaaggggct ctttccatgt
    25921 ataaactagg ctctcctggc tcatctaccc ttgttaagcg catatgaaga atctttactt
    25981 gagggtttcc ttgataaacc tctcttttac cgtttagaaa aacgactgtg ccggctgtaa
    26041 atttattgat ttcctcctcc gaagcatccc agtatttacc atcgatagta cctgaagtat
    26101 cttggaaagt aaaagcaata aatttttttc cgtttttagc gattcttaca tctgcacttt
    26161 tgatcaggac aaattgttca aactgctcat cgattactaa atcacgaatt tttttcatta
    26221 tttgctcctt ctaattgttt tacgatttgg ttcatctcat ggtaatagct taccatttct
    26281 ttatcagaag aaaaacaaat tatttgatac ttttctccaa atttagctaa aagttcagct
    26341 aattgatact ttcgctgatg atcataatga agccagccat catcaatgat gatcgggcat
    26401 atcgaacggt tagcttctaa tgagaggtaa gcaaatctca tcgccataat cagctgatct
    26461 ttcgtacctg tagataactc atagatggga aattctgtat atccgtctgt taaagtcagt
    26521 gtatcatttt tcagtaatat cttctgataa tttccttttg ttaaaatacc taaataatca
    26581 cctgctgctc ttaacaactg gggtaattgt ctttcactca tctctgttga aagatcacct
    26641 aagaatgtcc cggcgatttc aagtgctccc cattcctcca gtaattcctg taattttgct
    26701 ttttgccggc tagcttgttg ataaagttcg tccaatgtgc cgtctctttg caactcttct
    26761 acttgaagtt ggagtcgttg ccgattttca cttgcttctt tttcttcttg ctggagttgg
    26821 gattgctttt ctttcagttg gcgcagtctt acagataatt tctcttttgt cagctcttca
    26881 ggaaatagtg gagtaagcat ttccgataat tcattgtatc ttttcatctg ctgttcggct
    26941 tgtttggctt tttgtaaaaa gacaggtact tccgaaggat aagataagcc tgcttgagtc
    27001 aattgttcgc tgtattcttg tatccatact ttttgtttct ttttcaattg attgatttct
    27061 tgcttcaata aagtattttg ctgatagctt ctggcaaatt tcatctgttc catctcttgt
    27121 ataaaacggg aaagcaatac cattttttct gaaattgtct tatcttgtat cggtaaccat
    27181 tctttaacaa actcaaattt ttggtcgatt tgtcgtagtt tttcttgtat ttcctgttct
    27241 cttttatgtt tccgctcaaa tacgccatat aatttctgat aatctatgac ttcctgtcca
    27301 tattctttca tgagtttgag cgtatccatc tttcctaagt gccgatgatc cgcaaaacga
    27361 tatacttctt gctgaagaat ctgtttttct tctccagttc tcttttgttt ttcttctgct
    27421 tcagctaatt gagctaaggc tagatcaagt tgtcctagtt tttcttgcca cattcctttt
    27481 atctcagaga aatcattttt agttttaatt tttggttgcc ttttgtagaa gaaatagcca
    27541 atcatgcctg caccaattag taaaaggatc actttcaagg gcattggtac aaatatacct
    27601 gcaatgatta ttgctccgcc agccatcagc atatacggtt tagatgaaga gactggtgct
    27661 atctctgact gattttgtaa taatgctgga taggtctctt ccaagtcatt cacttctgct
    27721 tcaatcatct cttgctgttc tttagctatt tggagacgca cagaaatctg gctttctaga
    27781 tcagcaatcc ttctttcttc agccagaaga tgcttccatt cttcttcata taaaaattct
    27841 tccggtaacg gatttttctg ccaatgccac ctttgttcca aaatcttcaa ttctgaattt
    27901 gtttctctag tctcaatttg taatctttgc cattcttcgt ttaattgttc cgctgtaaat
    27961 tgaagttgct ggagatcttt tagttcctgc tcctgctcta aataaaaata atatctggct
    28021 gactgctgat ccatcccgct atgcttttcc agtgttgctt ctaattcttc caaacgctca
    28081 tttgtttgct gataccgttt ggagaaagac tccagatcta aaagaagttg ctcatcagga
    28141 agacctgtag tgctgttttt tttgagagat tgccattctt catataaaga aaaattaagt
    28201 tcttgctgtc tggctaatga aaattgtttg ttcgcctctt ttaattgtgc caataactgc
    28261 tgctgttgcg actcgtattc ctgttcttgt tggatcaact tgccaaaata agcttcctgt
    28321 tcttgttttt gatgaatttt ttcttgcagc tctttccatt catttaattt ttgattgatt
    28381 agtaattttt ggcctttttt cttgaataat tgttgtgctt tctgataata ttcttgccgc
    28441 ttttggaata cttgagaact gcccgtgatg cctaaagaaa tcagcgcatc atgtagctct
    28501 ttttctctca aatgatctag ttccatcaac tgctcttgct ggaaagtaaa tacctgctgg
    28561 aataactctt tcgtcaaagg tgcgatcagt ttttctagta actcatcact tcccacttga
    28621 tcccccagcc acaccttcga ttttccgcga ttttgctgct tgtagcgctc gacagcaaat
    28681 tcaccatata caggatgttt aagccatatt ttgccgccat aggctgagcc gtcttttggt
    28741 gtataatcct tttttttacg gccttttgaa gggaaaccaa ataatattgc ttgaataaat
    28801 tgataaatcg ttgattttcc tgcttcgttt cccccataga tcagctgatt gcctgaataa
    28861 aagtcaaaac ttttttgacg ccattttcca aaagcagcaa tctcgatcct taaaatcttc
    28921 attcctgatc ctcctcaaat aaaaaatctt gattaagcag ctgcttggct ttttcaagtg
    28981 tttcttcttg atattccggc agttcgctca atattctagc cgcttcttga tgactatata
    29041 cttcatttaa aatttgttgg aaatcttgag gtgtctgata gtattgaaat aactgttcca
    29101 tcaatttagc agaagcagca agtggggtct tcgtataaaa agtattctct atgagagaaa
    29161 tacgccaaat aaagaaatca tttatcctat ctgagaattc ctctaacaaa tagtctaata
    29221 gctctcctga ttgtatccgt tccaaaacat ctgtattgag atggtcataa tcttttaatt
    29281 ttatctcaat taaactaaat ccatcctcta cttttgaaag ctgctgacga atgtgaacaa
    29341 tgacctcttt tgtcgttctt gctttcctca atgatatttc ctttgtcttc caatagacct
    29401 ctgctacttt aatcggatgt atttggcaac ttcctttact taattcaact agtagaatag
    29461 atgtagaagc ttgttccttt ttagtatgac cttgaggcgc acctggataa acaattgttt
    29521 gcttttcatt taagaccgtt ggcacatgga tatgccctaa agcccaataa tcgtatccct
    29581 tttcttgcat ctttgagggt tggaaaggag catagtttcc tttttgagag acgccaggtt
    29641 ccccgtggta caacccaata tggtaatctg tgagttctct agaaggaaat tccatcactt
    29701 tgtctttttg aatccacggt tgacgataac taaatccact gagagagaca gtttctccac
    29761 tttttatcgt tatttttttt gtctctaccg tttcggatgt aaataaatgg acatttttag
    29821 gaaaggagaa ccaataccgc tctttttgat agaaatcatg atttccgaat attataaata
    29881 caggtatatg gttcttttct aaccgtttca tctgttctga aaaatgtttc tgtattttta
    29941 atgaaggacg attttgatga aaattatctc ctgcaagtaa aacaaaatca acgttattta
    30001 ttattgcctg ttcaatgata tttgataaaa cggtcagatt cgtcttcaat agtttttcct
    30061 gtacagtttt atcaagagtt gtcagtcctt caaatgaccg atccatatgt aaatctgccg
    30121 catggataaa tttcaccatt aaaaatttcc tccttttttt cttttatcat accattttca
    30181 atgttttttt tcatcaataa ccgaaaattt gttcgtatta tgcaaagaaa ttacgataaa
    30241 tggccgactt caaaaaataa gtttattcga aaaaaagatt gtgacatgat ttttgtcaca
    30301 atcctaaata tctatgttat attcgcttcg tttgacactg aagaagtact atttgtactg
    30361 gaatttatgc tccatttttc cgtatttaga attctatgtt ctatactacc ttaattagtt
    30421 tatatggtaa gaattctttc aaatgcttga gatactccgt ctttatcatt tgaaacagtg
    30481 acataatcag cttttttcat gatttcttga tgactatttc ccattgcaaa actaatatct
    30541 gcactttcaa acatagataa gtcatttaaa ctatctccga tcgcgattct caaaggtttc
    30601 tttaaattgt agattgattc caaacgatca aacaccacac ctttagatac attttgaggt
    30661 acgatttcaa tatttacagg tgccgattca gtaacagaca catttagcat ctttttcaaa
    30721 tggttaaata tattagcttt caattgacga ttaggaataa aaataaaaaa tttgtaaatg
    30781 ctgatagcat ccttttctat aatttcctca atattattat aagggattga acaaatagat
    30841 ttttcatact caagctcata gtttagctct ttgatagaaa attcactccc tacctcgtga
    30901 aaaatttttt tcattttatt tttataattc tgagattcat aacttccgag atttgtatat
    30961 attttggttg gaaatttcga taggacagaa tcaatttttt ttacatcatt tttatcgaag
    31021 gaagactcaa atatcttttt ttctccatca aatccaacag aaccatttaa ccctactgct
    31081 ggtatcctta aaccagattt tttgagaata ttattaatgt cttctacttc tcttccagaa
    31141 caaatgaatg gaaaacagtc ttccggtaaa tgtttcattg ttcttgcatt tttttgacta
    31201 atgctctggt cagaattcaa aagagtaccg tctaaatcta taccaattag tttcatttca
    31261 tccatgtctc ctatacgttg attataaatt cactgcttat tgtaagtcta aatttctcac
    31321 aaaacatata ctaattatac ctaatttaag taagtacaaa catcatttta agaataggaa
    31381 aggacattta tatactattc tctgtatttt tgatttataa ataaatatga attgaatcca
    31441 aatcagctat attactattt tataataacc aactataaaa aacgaagtta ggtgaaaaac
    31501 taacttcgat attcataaaa gactctatta gttcaactta gcaaaagatt taaacaaaaa
    31561 aagatgaact atttctagtt catctttttg cctt
//
LOCUS       DAZKPO010000029        30826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000029 DAZKPO010000000
VERSION     DAZKPO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.029"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..336)
                     /locus_tag="ACG8NZ_001263"
     CDS             complement(172..336)
                     /locus_tag="ACG8NZ_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538951.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            631..1959
                     /locus_tag="ACG8NZ_001264"
     CDS             631..1959
                     /locus_tag="ACG8NZ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0538952.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2161..2994
                     /locus_tag="ACG8NZ_001265"
     CDS             2161..2994
                     /locus_tag="ACG8NZ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0538953.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3010..3747
                     /locus_tag="ACG8NZ_001266"
     CDS             3010..3747
                     /locus_tag="ACG8NZ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0538954.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3902..4870)
                     /locus_tag="ACG8NZ_001267"
     CDS             complement(3902..4870)
                     /locus_tag="ACG8NZ_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0538955.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5102..5935
                     /locus_tag="ACG8NZ_001268"
     CDS             5102..5935
                     /locus_tag="ACG8NZ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0538956.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5969..6808
                     /locus_tag="ACG8NZ_001269"
     CDS             5969..6808
                     /locus_tag="ACG8NZ_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0538957.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6834..7394
                     /locus_tag="ACG8NZ_001270"
     CDS             6834..7394
                     /locus_tag="ACG8NZ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0538958.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7593..9314
                     /locus_tag="ACG8NZ_001271"
     CDS             7593..9314
                     /locus_tag="ACG8NZ_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0538959.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9479..10621
                     /locus_tag="ACG8NZ_001272"
     CDS             9479..10621
                     /locus_tag="ACG8NZ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0538960.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10637..11848
                     /gene="thiI"
                     /locus_tag="ACG8NZ_001273"
     CDS             10637..11848
                     /gene="thiI"
                     /locus_tag="ACG8NZ_001273"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0538961.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12421..13068
                     /locus_tag="ACG8NZ_001274"
     CDS             12421..13068
                     /locus_tag="ACG8NZ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0538962.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13197..13413
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13461..16106
                     /locus_tag="ACG8NZ_001275"
     CDS             13461..16106
                     /locus_tag="ACG8NZ_001275"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0538963.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16416..17729
                     /locus_tag="ACG8NZ_001276"
     CDS             16416..17729
                     /locus_tag="ACG8NZ_001276"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0538964.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17719..18372
                     /locus_tag="ACG8NZ_001277"
     CDS             17719..18372
                     /locus_tag="ACG8NZ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0538965.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18427..19119
                     /gene="radC"
                     /locus_tag="ACG8NZ_001278"
     CDS             18427..19119
                     /gene="radC"
                     /locus_tag="ACG8NZ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0538966.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19269..19469
                     /locus_tag="ACG8NZ_001279"
     CDS             19269..19469
                     /locus_tag="ACG8NZ_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0538967.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19723..21402)
                     /locus_tag="ACG8NZ_001280"
     CDS             complement(19723..21402)
                     /locus_tag="ACG8NZ_001280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0538968.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21404..21616)
                     /locus_tag="ACG8NZ_001281"
     CDS             complement(21404..21616)
                     /locus_tag="ACG8NZ_001281"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0538969.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22009..23739
                     /gene="efrA"
                     /locus_tag="ACG8NZ_001282"
     CDS             22009..23739
                     /gene="efrA"
                     /locus_tag="ACG8NZ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0538970.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23736..25496
                     /gene="efrB"
                     /locus_tag="ACG8NZ_001283"
     CDS             23736..25496
                     /gene="efrB"
                     /locus_tag="ACG8NZ_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0538971.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25591..25788
                     /locus_tag="ACG8NZ_001284"
     CDS             25591..25788
                     /locus_tag="ACG8NZ_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538972.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25941..26426
                     /locus_tag="ACG8NZ_001285"
     CDS             25941..26426
                     /locus_tag="ACG8NZ_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0538973.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26530..27123)
                     /gene="clpP"
                     /locus_tag="ACG8NZ_001286"
     CDS             complement(26530..27123)
                     /gene="clpP"
                     /locus_tag="ACG8NZ_001286"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0538974.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27303..27830
                     /locus_tag="ACG8NZ_001287"
     CDS             27303..27830
                     /locus_tag="ACG8NZ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0538975.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27921..28658
                     /locus_tag="ACG8NZ_001288"
     CDS             27921..28658
                     /locus_tag="ACG8NZ_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0538976.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28662..29579
                     /gene="miaA"
                     /locus_tag="ACG8NZ_001289"
     CDS             28662..29579
                     /gene="miaA"
                     /locus_tag="ACG8NZ_001289"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0538977.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29581..30810
                     /gene="hflX"
                     /locus_tag="ACG8NZ_001290"
     CDS             29581..30810
                     /gene="hflX"
                     /locus_tag="ACG8NZ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0538978.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10178 a         5273 c         6579 g         8796 t
ORIGIN      
        1 catgatgtcg taggttcgag tcctactgcc ggagtaaata aagcttatgc aaagaaaaag
       61 aaatccaatc acttgggttt ctttttcttt tattattatg atgaatcaag ctttctgttt
      121 ttgtcacgta tgttgtaatc tttacgaaag agtagagttg gctactcata atcaaccata
      181 cttcccatca tcgctaaata cgaaataaat cagcattcct acaccaatca aagcacaagc
      241 tgcaccaaga aaagggatga cattcccaaa gttatctgta ttcataagta tgccaccacc
      301 cacaaacaaa aagccgccag caatcaggtt tttcatttta ttcatctcct ttcttacgtt
      361 atagtatttc cttctttcaa tgagtatttt gaaagctatc ttattgtatg atgttcttct
      421 aggcctcttc tttattatac aggccatttt cgttatctca tagaaatatt cctttttatc
      481 tgaaaactat tgtataccgt atgacatttt tcttcatgaa gttgtaagta tattctcatt
      541 caaatgaaaa tttcatcata gaagagattg tatttctaaa ttcttaagag taaaatacga
      601 agtataactt ggatttggaa ggatgattgg atgaaagcta ttacgcctga tatgacaaca
      661 aaatttaaca aacagtttca agcaaatatc aaacagcagg cattgcagcg ggcagttgtg
      721 aagaacggaa ttactgcttc tgctgaaaat gtttcagcac atgtggagaa tacaccggta
      781 ttttctatag atttggctac ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg
      841 atgtttgctg cactgaacac attccgtcat aagatgctga atcaattcca tttaaaagag
      901 tttgaattat cacaaaatta cacttttttc tgggataaat atgaaaaggc aaattatttc
      961 tatgaaaaca tcattgcaac aggaaaagag gcaatcgata gccggaaagt agctttctta
     1021 ctcgctactc ctcaacaaga tggcggccaa tgggatatga tcgtttcatt attccaaaaa
     1081 tatggcgttg tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat
     1141 ttgaataact acttgaacaa attactgcgt aaagatgcag tagtgttacg gaaaatggtt
     1201 gctcaaggag aaacattaga cgctattgaa gaaaagaaag aagaaatgct agaaagcatt
     1261 tataacttct tagctatctc attaggaaca cctccaaaag aattcgattt tgagtaccgc
     1321 gatgaagaaa agaactatca tcttgaccga ggactgacac cacaaatttt ttatgataaa
     1381 tacatcggtg taaaattaga tgattatgtt agcgtgatca atgcaccaac aaaagacaaa
     1441 ccattcaatc gttcttatac agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc
     1501 aaatatctga atgttgatat ggaaactttc aaaaaattag cgattgctca gcttgaagaa
     1561 ggagaatctg tttggtttgg ttgtgatgta ggtcaatctt ctactagaac tagtggcatc
     1621 atggccttag atgcttattc aatggatgat ctatttgata cggattttac aatgactaaa
     1681 gctgaacgtt tagattttgg agaaagtctg atgacccatg cgatggtatt gaccggtgtt
     1741 gacttagtag acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt
     1801 ggaaagaatg gcttctttgt tatgagtgat gcctggatgg acgaatatac gtaccaaatc
     1861 gttgtaagaa aagatttatt gacgaaagag caattaaatg cgttcaatga agaaccaatt
     1921 gttctatctc cgtgggatcc aatgggtgct ttagcataga cgagtatttc aatgaagagt
     1981 atgtagtaaa gataaaaaga gctggcttaa gccagctctt tttatcttta aagtccattc
     2041 cctgtttttt atggtccctg tgctataatt ctaaataaag gataaggatt ggaaataatc
     2101 cttttatcat taaaaatatg atgacgaagt agaataaaat tgggagtagg ggggaaagtc
     2161 atggatatcc aaactgtttt atctgatctg aaattaaatg gtaaagtgat tccagttgtt
     2221 ggtggcgatg ttaatcagac ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa
     2281 atccatccga atgtaaaaaa aggatttttt gaagcagaag tagacggatt aaaagaactg
     2341 tcagcattcg tacgtgtacc ggatacgtat atgctaggtg aaacaagtga aggcgcttat
     2401 ttgttaatgg aatggatcga gccgggaaaa ggggatcaac gagatcttgc tgcagcttta
     2461 gctaatctcc atcagcaaac agcgccacaa tttggttttc gtaaagataa ctatttgggt
     2521 acacttgttc agaaaaattc ttttgaagaa gattggtgga cttttttctt taaagatcgg
     2581 cttgaatccc agatttcttt ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa
     2641 aagtatctgc gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa gatcactcca
     2701 cgcctcttgc acggagacct gtggagtggt aatgtgttct ttgaccagca agggcatcca
     2761 gtttttgtcg acccagctgt ttcttatggg aatcgggaac aagatattgc aatgagccag
     2821 ttattcggtg gtttccgacc ggaattttta gatgcttatc aaactatatt tccattagaa
     2881 aagggatgga aagaccgact gccgatttac cagttatatt atcttttggc acatttgaat
     2941 atgtttggag aaagttacgg ttcccaagta gatcaattac tagaaaattt ttaagaggag
     3001 ggcgtgtttt tggcaaaaaa atataccaaa aagcatgaag tttcatatta tgagtgtgac
     3061 atcaaccaaa cgatgacatt tccttcgatg ttaggagtag ttatcaaaac gtctgaagat
     3121 caaagcgacc agttgggaag aggcagtgaa ttcgtcaata gctttggact tacttgggtc
     3181 atcacgaatt attccatgga gattaatcgt ctccctagag tcggtgaagt aatctcagta
     3241 acgactcaag ccatggagta caataagttt ttctgttatc ggaacttctg gatccatgat
     3301 gaagatggga aagaattagt caaaatagaa tctgttttcg tattgatgga ttttgtgaat
     3361 cgaaaaatga gcagtgttaa tgaagagatc attgctcctt ttgagagtga gaagatcaag
     3421 aaaatcaagc gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt gccatatcgt
     3481 gttcgttttt atgatatcga tagtaatcag catgttaata atgcgatgta ttttaattgg
     3541 atcattgacg tattaggata tgactttttg actacgcatg ttccagaaaa agtaattatt
     3601 cgatttgata aagaagtaga gtacgggaat gagatcgaaa gccattttga acaaatccct
     3661 atcgaaaatg gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct gtattgtgaa
     3721 gcaaatatta tatggaaaaa taaataaaaa agaggttgtg acagaagggg gcaacttcaa
     3781 gaaataagaa gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt
     3841 ttcgaagaag ctatttttgt cacagcctct ctttttaagt ttagctatta gataatgatg
     3901 tttagttttg gataaacgaa atcaattctt cacccgacaa accatagttt acagcaatca
     3961 atagttttaa acgtgctttt tgactattaa tactgttaca gaaaataatc cccatttttt
     4021 ctagttcttt tcccccgcct tcataatcat aaacaggctc tgcgattcca ttgaaacaac
     4081 gagaaacaag tactacaggg attttttgga caagaagctt ttccaaagca gctaaggctt
     4141 gtggagggag attccccgca cctaaagctt cgattaccaa accgtctact ttcgtatggg
     4201 cgatggcttc caataagtcc ccttgcatcc cagcatatgc tttaacgatc gggatagtcc
     4261 catctacagc ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag aataaaattc
     4321 gattcttagt tactaagccg atgggaccca atgtaggtgt tcggaaagta gcaacatttg
     4381 tggtatgggt ctttgtcacg tagcgtgcag aatggatctc atcgttcatg accaccaaaa
     4441 cccccttatc tagcgcttct tcacaacttg cgacacgaat cgcactttca aaattataaa
     4501 ggccgtcact tccaagttca ttacttgaac gcattgcccc agtcaaaaca attggtaatt
     4561 tgttgccaat cgttgtatca agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg
     4621 tgatcaccac tccggatata cctgatgcat aagcggacaa gatacgttct tttaatacca
     4681 acatgtgaga aggcatgatg tgtggtgagg gaagattgaa gatatcttca actaataatt
     4741 ctacatgctt ggggattgtg atttctgcat ctaataatgg gtttgccaca tcaggactaa
     4801 caccgcccgt gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt agtacaagta
     4861 tttttttcat tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt
     4921 atactatata aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa
     4981 aaaagctgac cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta
     5041 tctggttgag tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga
     5101 aatgatcaga atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa
     5161 taacactctt tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta
     5221 tggaattccc cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg
     5281 ctttgtagtc aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca
     5341 gaccgaagaa gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg
     5401 aaagatgttt tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt
     5461 ccagtacttg cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg
     5521 ggattattct tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc
     5581 cgctttttct ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga
     5641 tccatttgtc accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg
     5701 atggcaagca aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa
     5761 agaactgcaa gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg
     5821 tgtgattttt aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa
     5881 aatattaaat atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct
     5941 tattttattc aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg
     6001 aacattggtc accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc
     6061 tcggaaaaac ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga
     6121 aatcattgat gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac
     6181 tgcagatcgc acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga
     6241 atttgcagat gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc
     6301 aacgacgatg ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg
     6361 taaattcccg gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa
     6421 ggatcgttat gaaacgttag agatattaaa agaaccaatt tatcaatgta tattgctcag
     6481 tccggagtca gaacagaaaa agttagaaca tcgctttccc gagtgtacgt ttcagcgttc
     6541 aaatacgtat actgtagata tcatcccaaa aggtggttca aaattactag gcatccaagc
     6601 tttcgcaaat gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg atcattacaa
     6661 tgatatcgag atgctgaaag gtgtaggaat tggagtggcc atggggaatg ctcagatcga
     6721 agtaaaacat gctgcagact ttgtgactca atcaaatgaa gaagatggga tatacgaagg
     6781 actgaaacac ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc agtatggaag
     6841 cagagacacc ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca agaagaccag
     6901 agatacccac ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag
     6961 aacttgttga atttgttgca gctacttcac ctaataaaga acaactgcat gaagctgtta
     7021 tgctgctgca cgaagcatta gataaagcag aagaaaaaat acttggtaaa acacagtggg
     7081 gaagtgatcc gctggttgaa caagtagatg cactgacaga tatcctttac tttacttatg
     7141 gttctttttc attactagga atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa
     7201 atatggggaa attatttcca gatggaaaac ctcattatga ccctgtaaca aataaagtgt
     7261 tgaaacctga aaactggcag atggaatatg ctccagaacc taaaatcgcg gcagaaatcc
     7321 aacgtcagat tcaagaagcg attgctaaga agaaatccgc tgatataggc atgtgcacaa
     7381 atgaaaaaga gtgatcagag tttcactatt tttgttatct ttatacgatg gaaaaacaaa
     7441 agattttcgt ttaagttagt gaatgcatga aaatagtatg ctataatcta gaatagatgt
     7501 cttttgcacg agagacgtat tatttggatt tatgccaagc agaacgccat ctgttgcata
     7561 aaagatgcat actgaaaacg gaggaaccat aaatgggaaa taatgttatc atcggaatcg
     7621 tcattgcaat cattatagtc gccattgtcg tctttttggt tagttacttg atgaagaaaa
     7681 aaaaccaaga tcgattggat gccttagaaa aacgcaagga agaattattt gacttgccag
     7741 tgattgaaga agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct
     7801 ttcgtgaatg gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct gaactagaaa
     7861 gtcaaatttt cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag
     7921 cagttgttga agcagaagct acaatggacc aaatggagaa acaagtgacg gaaatccgtg
     7981 aaggtctaaa ggaattgcgg gaaacggaag aacgcaactc actagctgtt cagcaggcac
     8041 ttgacgtcta tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa tacggaccag
     8101 ctctttttga attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg cagtttgtga
     8161 ctttaaacac ttctggagac cctgttgaag cccgcgaggt tttggataaa gcagaaaaac
     8221 atacgtatga agtggaagct ctgatgaaaa aaattccacc gctgtatgag gagctccata
     8281 ccactttccc tgaacaactt gaagaaatca gtgatactta cgagaaattg atggaagaac
     8341 aatatgtatt cccagaagaa gatcttgctg aagatatcgc aaaagtaagt cgaagaattg
     8401 aaaattcatt agccaatctt gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg
     8461 aaacagctga tttgattgat tcgttatatg atatcttaga acgggaaatg gaagctcaga
     8521 gatatgtaaa aactaaccaa tcaacgattg cagagtatat caaacataca acaaagaata
     8581 accgtcaatt attgattgag ctggatcaca catctcaaag ctatacattg aaccacaatg
     8641 aaattggccg tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt caaaatgaac
     8701 agatgattcc acagatccgt caacacgaaa ttccttattc agaagtgcgc acgttctata
     8761 aaacagtctt caaagtctta gaagatatcg aaaagcaaca agtagagatc gacgactcat
     8821 tacatgaatt gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca
     8881 aattgcgcag tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt
     8941 acctagaatt cttttttgtt gctactgacc gtattgaaga actaagtgtt gtcttgaata
     9001 aaattcgagt aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa gaagatctag
     9061 aattattaga taagaagaca catgatttag tagatgcagc agcattaacg gagcaaatgc
     9121 tccaatatgc aaatcgttat cgtcatacac acgaagaagt gagagaagca attgataaaa
     9181 gctactattt attcaataaa gaatatcact atcaagaagc attagacgaa atcggtacag
     9241 cattagagcg agtagaacct ggtgctttca aacgcatcga agatttttat tttaatcatc
     9301 cagacttagt ataaagcaaa aaagttagta aaccagcaag agcaagacgg attattgtct
     9361 tgttcttgtc tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt
     9421 gaaagcacct tggacaaccg ttataataga aaagaatttc aaaaaagagg ggtttcttat
     9481 gatatatttt gataacagtg caacaacccc aatctttccg caaagcttag atgcatatgt
     9541 aaaaaccagt cagcggatta ttgggaatcc atcaagcttg catgatttag gcaatcaagc
     9601 taaccggttg ttgcagcaag caagaaagca gatcgctgat ttgatccatg tagaaccaga
     9661 agaaatctat tttacttcag gcggaacaga aggagataac tgggtattaa aaggaacgat
     9721 gatcgaaaaa agagaatatg ggaatcatat gatcatttca ggagtcgaac atccggccgt
     9781 cagcgagaca gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga
     9841 taaacgcgga tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag agacagtatt
     9901 agtctctgtt atggctgtga ataatgaagt aggatcgatt cagcccatcc aggaaatcag
     9961 tgatcttttg gatgattttc ctaaaattca tttccatgta gatgctgttc aggcgattgg
    10021 aaaagtacca ttctcgcaat ggttgacaga aagagttgat tttgcaacat tttcagctca
    10081 taaattccat ggcccacggg gaactggttt catttattgg aaaaagggtc gtcgtttagc
    10141 accattactg acaggtggag ggcaagaaag aaatcagcga agtgggacag aaaatgttcc
    10201 aggtattgtg gctatggtta aagctttacg tttatcatta gagataaagg aacaaaaacc
    10261 tgagcagaca caacgcttaa aagtagctct aatcaatgcg ttgaagacgt atgagaaagt
    10321 gacgattttt tcagaaggaa ctgactatgc accgcatatt ttatgttttg ctctaaaagg
    10381 aattcgcggc gaagttctcg tccatgcctt agaagaaaaa caaatatttg tttctactac
    10441 tagtgcctgc tcctcacgta aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc
    10501 aaatgatctg gctacatctg ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga
    10561 agtcgagcaa tttatgatta ttttcaagca gctttataag aaattttctg caatcaatta
    10621 aaagaggagt gttcttgtga aatacagtga aattatggtc cgttatgggg aactttcgac
    10681 aaaaggaaag aatcgaaaaa gctttatcat gcaactagca caaaatgtca gacaagcttt
    10741 gaaagacttt ccagaaatca aaatccatgc tgatcgggac cggatgcatc ttttattgaa
    10801 tggcgcagac gggcagctag ttattccaaa actagcaaaa atatttggga tccaaaattt
    10861 ctctccaagt attcgtgtag aaaaagatgt cgaagttttg aaacaagctg ttcaagacat
    10921 catgaaagaa atttatactg ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca
    10981 ccaatttgaa ttgacaagta atgaattgaa tcaaacatta ggtaatgctg tttttgatat
    11041 tttcccgcat atccaagttc aaatgcgtca accagatatt ccattacgtg tagagattcg
    11101 gcgtgatggc gcttatctat cgtatgaaac gatcaaggga gctggaggat tgcccgtagg
    11161 aacaagcgga aaaggaatgc ttatgctttc tggggggatt gattctcctg tagcagggta
    11221 ctttgccatg aagcggggag tggaaattga agctgttcat tttgctagtc cgccgtatac
    11281 aagtgaacaa gcactgcaaa aagcaaaaga tctaacagcc aaactagcac cttatgtggg
    11341 aacgatccat tttatcgaag tgccatttac cgagattcaa gaagaaatca aaaagtcagt
    11401 gccacaagga tattggatga caattactcg tcgaatgatg ttgcgtttga ccgatgaaat
    11461 tcgccgtatt cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc aagtggcttc
    11521 acaaactttg caaagtatgg tggctatcaa tgaagtaacg aatacgccaa tcattcggcc
    11581 tgtagcgaca atggataaac ttgaaatcat tgaagtagca gaacagatcg atacatttaa
    11641 tctttctatc cagccttttg aagattgctg tacgattttt gctcctccac agccaaaaac
    11701 acgccctcaa ctggaaaagg tccatcaata tgaagagcgt ttagcaatag atgaaatgat
    11761 ccaacgagca ctagctggct tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa
    11821 aactgaagaa ttttcagaat tgctataaga ggttatgaaa atagtgtttt gaccagagta
    11881 ttagagaaac aaaacgaaaa aatagctcca tgtattttac cgtttttgaa ctaatgtcga
    11941 agattagctg attgaatacc atttatcaag aagttatgga aaagccggtt cttttttact
    12001 aactgaaaaa taaaatttta tttagagatc aagctacagt tgataaatga tataaaaaag
    12061 agaatttccc atgaaatagc gaggttgtga cagaagtggg cagcttcaag aaataagaag
    12121 gaatttacga aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt
    12181 tacttctgga acaccgtgta tcagaggttg cgaaaaagct gccctgcatc aagtaataag
    12241 aacagccaaa caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat
    12301 gcaagctttt gaacaccgtt ttatttgtct cacacccttt tccttcctac ttgtaacctt
    12361 gctcccgtgt tagaaacatg ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt
    12421 atgcgagaaa aaacagatac actgccaaaa gcaactgcga aaagattacc tttatattta
    12481 cgctacttga agatgctgga tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc
    12541 gaaatcaccc aaattccttc tgcaacgatc cgcagagact tctcgcaatt aggcgagcta
    12601 ggcagaagcg gatatggcta tgatgtccct tttcttatcg atgtattcaa taatatcctt
    12661 aatactaaag aagaaaaacg aatcgcatta gtaggatatg gaaatcttgg caaagcattg
    12721 aaacacaaca attttagaag aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat
    12781 ccagcactga tcaatcgagt aattgatgga gaaatgatct atccgattga tcgtttcgcg
    12841 gagatagcca aagcaaaaaa tgtgacagta gcaatctcta cggtaccaag taaatattca
    12901 caatctgcta ttgatgaaat tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct
    12961 gacagagtaa ctgtcccagc ttatgtcaat gttcaataca ttgatttgac aaccgagttg
    13021 cagacgctta tttactttga tgagaattac tcagaagttt tttcttagga aaatttgagt
    13081 aggcttattg atcttgcgag aactcgtcgt aattctttca aaaatttttt agtactaaat
    13141 gaagtggttt tttcacgacc acttgttgac taaaaaaaaa aaccattgta gaatgaagca
    13201 taacgctgaa caagaggagt aactggcaca ttcattctta gagagagaac tatctgctga
    13261 aaggttctca atgacaacca gtgaaggtag cttgggagtt tctgtcttga atggagtaga
    13321 gatagacgag tcgtgatcgt taactcactt gagaggcaat aatgattgat tattgcaatt
    13381 taggtggtac cgcgtaaatc atacgtccta gaatacgaaa gtattttagg acgtttttta
    13441 gtttaaagga ggaaaacagc atgacggaag aaaataacct atcaacaaaa tataatccgc
    13501 aagaagttga agctggacgt tatcagcatt ggttagaaca agatttgttt aaaccgagcg
    13561 gagataaaaa agcaaaacct tattcaatcg taatcccgcc cccaaatgtt actggaaaac
    13621 ttcatttagg gcatgcttgg gatacgacct tgcaagatat gctgattcgt caaaagagaa
    13681 tgcaaggata tgatacttta tggctgcctg gtatggacca tgcaggaatc gcgacacaag
    13741 caaaagtaga agcgaagctt agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa
    13801 agttcatcga tcaagtgtgg gaatggaaag aagagtatgc gagccacatc cgcgaacagt
    13861 gggctaaaat gggcttatct ttagattaca gtagggaacg ttttacatta gatgatggat
    13921 tatctgaagc agtacgtaaa gtttttgtta ccttatacga aaaaggtttg atttatcgtg
    13981 gtgaatatat catcaactgg gatcctcaag cacgaacagc tctatctgat atcgaagtta
    14041 ttcataaaga tatcgaaggc gccttttatc atatgagcta tgaactagca gatggatcag
    14101 gcgtagtaga aattgcgaca acccgtccag aaacgatgtt gggagatacg gcgattgctg
    14161 ttcatccaga agacgagcgt tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag
    14221 tcaataaaca aatcccaatc attgcagatg aatatgtaga tatggaattc gggacaggtg
    14281 tagtaaaaat cactccagca catgatccaa acgactttga ggtaggtaat cgtcatgatc
    14341 ttccacgcgt aaatgttatg gatgatgatg gtacgatgaa tgaactagca ggaaaatatg
    14401 ctggaatgga acgttttgct gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc
    14461 gattgattaa aatcgatacg atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg
    14521 ttgttgaacc tcggctgtct actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag
    14581 ccattgataa ccaagaaaca gatcaagcag tggaattctt ccctccaaga ttcaatcaaa
    14641 cgtttttacg ttggatggaa aatgtccatg attgggtaat ctcacgacaa ttatggtggg
    14701 gacatcaaat tcctgcatgg tatcacaaag aaacaggtga aatgtatgta gggatggaag
    14761 caccagcgga tagtgaaaac tgggtacaag atcctgatgt tttagacact tggtttagtt
    14821 cagctttgtg gcctttctca acaatgggct ggccagatac ggaaagcgaa gattataagc
    14881 gatacttccc tactagcact ttagtaaccg gttatgacat cattttcttc tgggtaagca
    14941 gaatgatttt ccaaagcttg gaatttactg gcgaacgtcc atttgagaat gtcttgattc
    15001 atggattgat tcgtgacgaa caagggcgga agatgagtaa atcattaggt aatggaatcg
    15061 atccgatgga agtaatcgaa aagtatggag cagatgctct ccgctggttc ttatcgaatg
    15121 gttctgcacc aggacaagat gtccgcttca gctatgaaaa aatggatgct tcttggaact
    15181 ttatcaacaa aatctggaat gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg
    15241 tagaagagat cgatttatct ggagaaaaat cagtggctga ccggtggatc cttactcgtt
    15301 tgaatgaaac gatcgaacga gtaactgagc tatttgatcg ttttgaattt ggtgaagcag
    15361 gtcgacaact gtataacttt atctgggatg atttttgtga ctggtacatc gaaatgagta
    15421 aagaagtact ttatggagat gatgctgttt caaaacaaac cacccgtagc atcttagtcc
    15481 atacacttga tcagatccta cgtttattgc atccaatcat gccattcgtt acagaagaaa
    15541 tatgggaaca tattcctcat cagggaaatt ctctagtagt ggcagaatat cctgttgtac
    15601 gtccagagtt tgatgatgaa acagcttcta aagggatgga agtattgaaa gaattgattc
    15661 gagccgttcg aaatattcgt tcagaagtga atacaccatt gtctaaacca attacgttgc
    15721 taattaaaac aaacgatgct aaaatcgatc aatttttagt tgaaaataca agctatatcg
    15781 aacgcttctg taatcccgaa gagttgacga tatcaagtga gatcactgcg ccagatctag
    15841 cgatgtcagc tgttctgaca ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata
    15901 ttgaagaaga aatcaaacga ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac
    15961 gtgtccaagg aaaactttct aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag
    16021 aggcagagcg tgcaaaagaa aaagattatt tagagaagca aacggctgtc aaagaacgaa
    16081 tcgaaagttt gcgtactatt caataagaat agaaaaagag agtgtgaaat cagctagcta
    16141 ttactaaaag ctagctgatt gaacatcgtt acaggaaaga gtttgagcct cactcttagc
    16201 ttcgattcat aaggagatgt gacataaatt ttgtcgcatc tcttttttag tctactctta
    16261 ttatgtagga gaaggaggga ggtggatcat gtatcaagag tcaataaagt agttgataaa
    16321 atcgttcctc tctctgtttc ttttctcttt gtcatacttt tatttttctt atgtgttcta
    16381 tttgttataa taaaagagaa atgggggaat tgttgatgac gattgaagaa gcaatagaat
    16441 ggatccatag tcgattacca tttggttcac gtcctggcct tgaccgagtc gaagctttat
    16501 tagaagtggt agggcatcca gagagaaaag taaaaacgat ccatatcgct ggaacaaacg
    16561 gaaaaggttc tactgtagct tatttgagaa gcctattaga agaggctggg ctgacagtag
    16621 gtacatttac ttcaccttat atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa
    16681 gcattccaga caatgatttg atccagcttg taaaaaaata tcagccactg atcaaacaac
    16741 ttgatcaatt tgaagcagta gctggtatta ctgaattcga gacattaaca gcaatggcat
    16801 ttgattattt tttgatgaag aaagtggacg tagcgattat cgaagttggc ttaggcggct
    16861 tattggattc taccaatgta gctgtaccaa tgctgacggg aataacaacg attggacttg
    16921 atcataccga aatacttgga gatacaattg aagaaatcgc tgcacaaaaa gctggaatca
    16981 tcaaacaagg cgttccagta gtaacaggaa atattgcccc agaagctttt actgtcatcc
    17041 aacaaacagc taaagcaaaa aatgcaccgc tttatgtata cggggaaaat tataaaataa
    17101 actatctgca tcctgatgag atgtggggag aagtatttga attttttaat gaaataggaa
    17161 agatccctca attaaccact ccgcttttag ggcaacatca agctgaaaat gcaggagtag
    17221 ccatcgaatt gtattttgtt tactgtcaat tggaaaagct gcctttccga gaaaaagatg
    17281 ttttgaaggg attgaagaaa tctcgatggc caggaagaat ggagcgtttg agtaacgagc
    17341 cattagttgt tttagatggt gcccataata cgcatgcaat gaaccggctt gttgagaatt
    17401 tgaaaaaaga attcaaagga tatcaaatca acattttgtt ttccgcctta cgaactaaaa
    17461 atgttagaga aatgctagaa cgattgcagc aagtaccaaa tgtccatatt tatttgacga
    17521 cctttgaaca tccaaaagcc atcgaattaa gtgaagtatc agatttagca agtgagagag
    17581 tatcaatcgt ttcattgtgg caattcggat tagcagatat attggaaaag atgacaaatg
    17641 aagacgtatt agttgtgacc ggttcacttt atttcatttc agaagttcgc catttattaa
    17701 tacaattagg aggagatcat gaagtataat gcagtgattt ttgacatgga tggtttgctg
    17761 ttcgacacag agttagtgta ttacgaagcc acacaagtag tagcagacaa gatgggtttt
    17821 ccttatgata aagacctgta tttaaaattc ttaggtgttt cagatgaaga agtatgggcg
    17881 aattatcata tgattttctc ggaatttggc aaaacaagtg tacaaagatt catagatgat
    17941 tcataccagg aaacgcttga acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg
    18001 ttggaactcc tctctttttt agaagaacat cagattccca aagttgtcgc ttccagtaac
    18061 caacgagcag tcattgaact tttattgaag aagaacaatc tgtttgaacg atttgagacg
    18121 attgtttctg cagaaaatgt tcaacgagca aaaccagacc cagaaatttt cttgttagct
    18181 aatcagaagc taggtacagc gaagaaagaa acattgattt tagaagattc acaaaatggt
    18241 attttagcag cttctgcggc tgagatcccg gtagtcatgg taccagatct gcttccacca
    18301 tcccaagaat tgatcaaaaa aacagctggg gttgtttctt ctttacatga aatccctgca
    18361 ttgttaaaat aaaaatccat ctcttttcgt atcttatttt agataagata gaaaggagat
    18421 gtttttttgg gaaaacgatt gatcaaagaa gtacctacta gctctttacc aagggaacgt
    18481 atggaaattt atggcgcgga agctttatcg gatcaggagc ttttggcaat cttactgcgt
    18541 accggtcaac acccttatag tgtgatgtcg attgctggaa atctgttaaa aacgtttgga
    18601 ggactggcct ctttacgaca ggctactttg catgagttag aagagattcg agggatagga
    18661 cgcgtgaaag caatcgaaat caaagccttg atcgaattag gtcgacgaat tcagacggat
    18721 catgaaagag cggctccacc ggttcgtacc agctacgaat tggcccagca attgattctg
    18781 gaaatgaaag atcacaaaca agaacatttg gtttgtattt atttggacac gaagaatcaa
    18841 gtgatcttaa aaaaaacagt gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga
    18901 gaaattttcc attatgctgt acgttattgt gcagcaagga tcgttttagc gcataatcat
    18961 ccttctggta atgttattcc atcgcagcag gatatgaatt ttaccaaaag gatacaaaag
    19021 tgtggagaaa tgatggggat cactgtactg gaccacttga tcatcggacg caaacgatat
    19081 tttagtttga gagaagaagg aatgatggaa gagaagtaag ttgctttctt gcaaaaaaaa
    19141 tgaagcaagt gtacaattat attgtaattt tgtttgggaa tagaagttga aacatctgtt
    19201 gcacctctat cgtacacgag caatgttaca atttaaatta gtgcttttgg cacgaggagg
    19261 atattattat ggaaaacgga acagtaaaat ggttcaacgc tgaaaaagga tttggattta
    19321 tctcacgtga agatggaagc gacgtattcg ttcacttctc agctatccaa ggtgacggat
    19381 tcaaaacatt agaagaaggt caagcagtga ctttcgacgt tgaagaatca gaccgcggcc
    19441 cacaagctgc aaacgttgta aaagcttaat tttatctgta aatcaaattg atggtagaca
    19501 tcaattttta cgagagaaca aagcgagtaa caaaaatacc tgccacaaga gacaatttct
    19561 cttgtggcag gtattttttt gtatgaaaaa gtaaataaat ttaagaggct atgaaagagc
    19621 caccttgtat caaggaataa gaacaaacaa aaatggcttc tcatgttttt catttgtttt
    19681 ctcttatatt caggacaagg tgctgatttc ataacctctt atttattctt catctggtgt
    19741 cattaccatt ggcaaaatca ttggatgtct ttctgtacgt tcgaataaga aaggctgaag
    19801 cgcgccagtc atcgcttctt tcaatttata ttctgaacag tcaggtccat tcaaacattc
    19861 tcttaaagcg tggaataaga ttcgctgagc ttcattgatc atttctcctg attctcgcat
    19921 gtagacaaat ccacgagaga gaatatctgg accagccaag atttcttttt tcttgatatc
    19981 cactgtcgcg acagctaata caagaccttc ttctgaaagc aggtgacggt cacgtaaaac
    20041 gacattacca atatcaccga tcccgctacc atcgatatac acatctccag ccacaaaatg
    20101 cccagcagga cgcgcagaat cgctagttaa cgctaaaaca tcgccatttt ccatgatgaa
    20161 gcagttttct gcaggaacac ctacatcttg tgctagagaa gcatgtactt tcaacatgcg
    20221 gaattctccg tggacaggaa taaaatattt tggcttcatc aaacgaagca ttaatttttg
    20281 ttcttcttgt ccaccgtgac cggaagtatg gatgttattg atttttccgt gaatcacttc
    20341 tgcaccagct tcagacagca aattgatcaa atgattgaca cttgttgtat taccaggaat
    20401 aggtgaactt gaaaagatca ctgtatcgtc tggctggatt tggatttgac ggtgagtacc
    20461 attcgcgatg cggctcaacg cggccatcgc ttctccttga gaacctgtac ataggatcat
    20521 cgtttcattg gcaggcaagc gattgatttc gtgtgcatct acaaatgtgc cgtctggaac
    20581 tttgatatat cctaaacgtt gaccgttgac gatggcattt tccatgcttc gtccgaaaac
    20641 agcgatttta cgaccagttg ccacagcggc atctgctgct tgctgcaaac ggaagatgtt
    20701 agaagcaaag ctggcaaaaa tgatacgtcc ttcgattttt gaaaagatct tcaaaataga
    20761 atgcccaatc gttttttctg atttagtaaa tgttggaatc tcagcatttg tactatcaga
    20821 taaaaggcac aagactcctt cttccccgat acgagccatt ttatgcaagt ttgctggttc
    20881 gccaacagga gtgaaatcga atttgaagtc tccagtcgct acgatatttc cagaaggtgt
    20941 tttcaccacg atccctaatg catctgggat actatgggtc gttctgaaaa agctgacaga
    21001 agtctttcta aaacggatga tcgtatcttc attgatctca tggagctctg catctcgaag
    21061 caagccatgc tcatctaatt tgtttgtgat caacgctaat gcgagaggac cagcatagat
    21121 aggaatattt gcttgtttca gcaagtaggg tattccgccg atgtggtctt cgtgcccgtg
    21181 agtaatcacc aaggctttta ctttgtgtaa gttttgtatg atgtagctgt agtcgggaat
    21241 gacatagtca attccaagta aatcgtcttc cgggaatttg atcccggcat caatcaaaat
    21301 gatttcatct tggaattgta ccccatacgt atttttcccg atttcaccta atccaccaat
    21361 agcaaagacg cctgtctcgt tatttttgat ggaaagtttc atattaaaac tccactaatg
    21421 agaaatcagc attttctttt tcgtactcta gatgacttcc ttctagaagt tgaacgaatt
    21481 caatattgta tggggtattt tcttccacac gttggcgagc gtctacatcg ctttttgcct
    21541 caatgtaaag agattttgtt tcttcccgtt tagggttgcg tacttttgtt tcttgataat
    21601 aaactttata gatcattgtt ttgtctcctt tatctgcgta aagcagtgat ttagctagtt
    21661 gattttttta gacaacacaa aacgagggga caagccacct ggtttttcat atgctttgac
    21721 tagaatactg gattcgagtc aaagagacaa gcctcgtttt tgcttatctg ttcggatatt
    21781 ttttcaaccg taccttctgt ataaaaaata tacaacaatt aattgttatt ctagcataaa
    21841 acagcaaata agtatagaaa tcaactagat tgaaagaaaa aatcaatgtt tatcatcccg
    21901 ctataatcat aatgactttt ttatgagaga agtgttctat taataggctc gttgtgctat
    21961 catttttagt aatgaaaatg taacaaaaga ttaaaaggag atgagaaagt gaaactaatg
    22021 tggcattata ccatgcgtta taagaaattc ttgtttttca atttcatatg cgtatttggt
    22081 tttattttga ttgaattagg acttcctacg attttagcac gtatgatcga tgtaggaatc
    22141 cgcaatgatg attttgatta tgtgaagcaa caagggatat ttatgatcgt tattactgta
    22201 atcggtatta ttttgaatat cttgttaggt tattttggtt cgaggattac gactaatatc
    22261 gtagcagata ttcgagatga tttgttcgaa aagatccaaa gttattcgca tcaggaatac
    22321 gaaaatctag gcgtatcttc attgatcact cgtacaacaa acgatgctta tcagatcatg
    22381 ttgttcctgc aaaatatttt acgtatcggt tttatggcac caatgatgtt cattgtcagt
    22441 ttgtacatgg tcatgcgtac aagtgtgaca ctcagtttgt atgtagtagg tgcattacct
    22501 ttactattat tggcagtcgt agcgattgct aaggtgtctg aaccgttatc taaaaagcaa
    22561 caaaaaaact tagataaaat caacagtatc ttgcgggaaa atctttcagg attgcgtgtg
    22621 attcgtgcat tcgtcaatga aaagtttgaa gaaaaacgtt ttgctgtagt caatgagaat
    22681 tatgcttcta gttcaaaaag cttattccgt ttgatggctg ttgcacaacc gggtttttat
    22741 ttcttattta atatcgtaat ggtattgatc atctggagcg gggcagtgca aatcgatgca
    22801 ggcgagcttc gtgtaggaga tttgattgcg tttatcgagt atatattcca tgccttgttc
    22861 tcattcatgt tatttgctag tgtgttcatg atgtatccaa gagcagctgt ttcagctgga
    22921 cgtatccaag aagccctaga tgccaaacct tcgattgtag aagatccaaa tggtatatct
    22981 gatacaaaaa ctaaagggta tatcgaattt caaaatgtaa catttgctta tccaggccat
    23041 tcgcaagagc cagtgatacg aaatgtcagt tttacagcct ctccaggaga gaccgttgct
    23101 tttattggaa gtaccggtag cggaaaatct acgttgatcc aattgatccc tcgcttttat
    23161 gatgtgagtg aaggaaagat cttgatcgat ggagtagacg tgagagatta tcggctaagc
    23221 aagttgcgag ataagattgg gtttattccg caaaaagcgc tgctatttac tggaacgatt
    23281 gcagataatt tgcgatacgg gaaagaagat gctacgcaag aagaaatgga acgtgcagct
    23341 gagattgctc aagcttcaga attcatttct cgaaaaccag atggatttaa tgaacattta
    23401 tcagaaggtg gttccaattt ttcaggtgga caaaaacaac gattggcaat agccagagcc
    23461 atcatacgcc gtccggaaat ctatattttt gatgacagct tttctgcatt agattatcaa
    23521 acggatgcga agttacgtgc tcgtctgaaa aaagaaacga cagaatctac tgtattgatc
    23581 gtagcacaac gtgtgggaac gatcatgcat gcagataaga tcatcgtatt aaatgaagga
    23641 gaagtcgtcg gtatggggac gcatcgggag ttgttggaaa aatgcccgat ttattatgat
    23701 attgccgctt ctcaattatc aaaggaggaa ttagcatgaa aaaaaccttt tcttccttaa
    23761 aacgtttagc tcgatatatc agaccgtatc gtgagacttt tcttttagtg atcctattta
    23821 cagttctgac agtggctttc aacgtggctt tgccatatgt tacaggactt cctactacag
    23881 aaatcagccg taatattgcc aatggcgaat caatcaattt ttcttatata ttcaaatgtc
    23941 tcatttggat cacagttgtt ggaatagggt attgtatttc gcaattactt tctggtgttt
    24001 tgatgacaaa tgtcgttcaa agtgccatgc atgatttgag aagagacata gaagaaaaaa
    24061 tcaatcgtct tccggtttca tatttcgata aaaaccagca aggaaatatt ttgtctcgtg
    24121 tgacgaatga tgtagatgca gtcagcggtg ctttacaaca agcttttatt ggtatcgtaa
    24181 atgcgatatt gggaattacg ttggctgcag caatgatgtt ttatatccag ccgatgatgg
    24241 cgctgatctc tatgatcatg attccaacat cgatctggat ctcaaaacga gtgatcaatg
    24301 cttcgcagaa atattttcaa agcatgcaga attctcttgg tgagctaaac ggctacgtcc
    24361 aagaaaatat gacaggtttt agtgtattga aagtatatgg acgcgaaaaa gaaacattgg
    24421 aaggattcaa aaaagttaat cacagcttga aatactttgg gtttcgtgca gcattcattt
    24481 ctggcttgat gatgcctcta gttcaattaa ctgcctatgc tacttatatt ggtatggctg
    24541 ttctaggaag cttttatgtt attactgggg cgatcgttgt cggtcaacta caagcattca
    24601 tccaatatat ttggcaaatc agtcagccaa tggggaacgt gacacaattg tcttcagttt
    24661 tgcaaagtgc atcagcggca acgaaacgtg tattcgagat tttagatgag ccagaagaaa
    24721 aagaaaacaa agtagatgct tcactgccag aaacgatttt aggagatgtg acattcgatc
    24781 atgtcgattt tgcatatgat ccgaagaaac cattaatcca agatttgaat tttgaagtga
    24841 aagctggtca aaccgtagct gtggtcggtc cgaccggtgc cggcaagacg acattgatca
    24901 atctattgat gcgtttttat gacgtagaca aaggagcaat aaggattgat ggaatcgaca
    24961 ccaaatcaat gagtcgcagt gatgtgcgtt cgctatttgg tatggtcttg caagatgcat
    25021 ggctttatca aggaacgatt gcagataata tccgcttcgg aaagcttgat gcgacagatt
    25081 atgaagtagt tgatgctgcg aaaacagcaa acgtagatca ttttatccga acgatgccag
    25141 atggatatga tatggccatc aattctgagg gagacaatgt gtctttagga caaaaacagt
    25201 tgctgacgat tgcccgagca gtggtctccg atcctaaaat cttgatttta gatgaagcaa
    25261 caagttcagt agacacccgt ttagaagcgt tgattcaaaa agcaatggat cgcgtgatgg
    25321 agggacggac aagtttcgtc atcgctcacc gtttatcgac aatccgtgat gcagatctga
    25381 ttctagtgat ggatcaaggt cagatcattg aaaaaggaac gcatgagagt ttattggcac
    25441 aaggtggatt ttatgaaaaa ctgtataaca gccaatttgc tgaggaagca gaataagtca
    25501 tataagaata aaacagttta aaacttattt tttgttctct attcttgata atggtaaagt
    25561 aaagttatca agaaaagtga ggacacggag atggaacagt ttttaaatga aaaaattact
    25621 gaagccaaaa gagaagttga tcgtttaacg cacgtctgtc agacgaagcc ttttgattca
    25681 gctgattttt ttgaaaatga gtttcaaatc gaacgcttgg cagcacaaat agaagtgtac
    25741 gaattaattt taaaaaaagt aatagaagaa aatatagatg tacaataaaa acaggtaaaa
    25801 cgtcagttac cagatactgt aaatagacat ctggcctgag caggttttac ctgttttttt
    25861 tcttatgaaa taaatatatg ctatgatacc tttattcaat aggattagtt tctacaaaaa
    25921 atgaaaggag aaaaataaat atggaacggt ttatagcatc aaatgccaca gtgattggtg
    25981 atgtgacatt gtcagaagat gtgacgatct ggtatcaagc tgttttacgc ggggacagta
    26041 attggataaa aataggacaa agaacgaata tccaagatgg tacaatcatc catgtcgatc
    26101 atgatgcacc tgtagatatt gcggagaatg tgacggtagg acatcagtgc atgctacatg
    26161 gctgtacaat cgaaaaaggg gcgctgatcg ggatggggac cacgatattg aatcatgcgg
    26221 ttattggtga gaatagtttg attggtgctg gatcattagt aacagaagga aaagttattc
    26281 ctcctaatgt cttggctttt ggtcgcccag ctaaagtgat ccgtcctttg acaaaagaag
    26341 aaatacaaaa aaacaaagaa aacatccagc attatgtcga gataggtcgg atgcatgcgc
    26401 aaggaaaatt caaggagcaa agataggcag tagcatccat aaaataaaaa aggatggtca
    26461 gtttgagttg accatccttt tttatttggt ttagataaga gagcttaaaa aagcttcctt
    26521 tgaaacagat taatttaatg aagaactgtt ttccatgact tcatcgatca atccatatgc
    26581 ttttgcttgt tcagctgtca tatagttgtc acgatctgta tctttttcaa tcacttcaag
    26641 cggttgtcca gtgcgttcag ctaaaatttt gtttaagcgt tcgcgtgttt gcaaaatatg
    26701 gcgtgccgca atttcgattt cagttgcttg cccttgagcg ccgccaagag gttgatggat
    26761 catgatctca gcatttggta aagcaaatcg tttacctttt gtaccagctg tcagcaagaa
    26821 gcttcccatt gaagcagcca tacccataac aattgtttgg acatctgctt tgacaaagtt
    26881 cattgtgtcg taaatagcca tacctgctgt aactgaacca ccaggagaat tgatataaag
    26941 atagatatct ttttcagaat cttgagcgtc taagaaaagc aattgggcga tgatcgaatt
    27001 agcaagatca tctgttactt ggccgcttaa catgatgata cggtctttca ataaacgtga
    27061 gtagatatcg taagctcttt cacctcgtga agattgttca atgactgtag gaattaaatt
    27121 cataatagag ttcctccttg atttcgttta gaaatagtgt accataattt agtttctaat
    27181 acctaaaatg tagtatacat ttttggtcaa aaaaggtcaa ataaaaaact taggcaaatt
    27241 agtcgcataa acccgcttta tggtatgata agcagagaaa caaaacaagg aggaacaaca
    27301 gattgatagt tggtattact ggccttatcg gagtgctgac aggtgctgta gttgtctata
    27361 tcggcatgat actgcaaatg cgtcacttgg aaaataaaga acagcgtaga cgtgaattgg
    27421 aattgaaagt caaagaaatc gagacgctga atcaattaaa taaaaagatc aacgagatct
    27481 tgcaaaaacg gacaacgctg atggaagaat atgtcagctt tgatgcattt gatgattgtt
    27541 atatcacgat tgacgatttt gcgtatctcc aatcattttc agcgcaaaat agtttttatc
    27601 tgccaaatta tattttagac gagctattta agaatatctc ccatcgaaaa gtgattttat
    27661 ctccagatga aacaatgaag attggtggat atgcttataa aggcggacgc gtgattttag
    27721 agaattttac ggatagtctg actgaaatga tcaatgaaaa aaaagctcag ttgaagacta
    27781 tgacgcgcca gccgattggt ttcttctcga aagatgatgt ttggagctga tattttgaag
    27841 cattgccgtt gcacatgaga gcgtggtaat gctttctttt tttcttaatg atacaataga
    27901 actgaacgga gtgaagaaag atgttacaaa tctatgccca tcgcggaagc agcggcaccc
    27961 acccagaaaa tacgcttcct gcatttgcag aagctgtacg agtaggtgca gatggtatcg
    28021 aactagatgt ccatctaagc aaagatggtt atttgatcgt tatgcacgac gaagaagtgg
    28081 accggacgac taacggtaaa gggctgattc gagaaaaaac attggaagaa ctcaaaaaat
    28141 tgaatgcagg gggcaaattc agcaaagaat ttccagcagc taaagttcct gaattgagag
    28201 aagtgttagg actgctcctc caaaaaaatt accgagggat gctaatgatt gaaattaaaa
    28261 ctgatcagta tgaatatccc ggtatcgaag aaaaattagc cgaaacatta aaaagtagag
    28321 attggccttt tcaacatgca tattgcagct ttcatttgga ttctttggaa aaaatcgcat
    28381 tactggaacc ggagatacag ctggacttat tgatgagtac ttctgataaa aagatccaac
    28441 tagcagcaga aaccccttat atagaaggag tacatccaaa aaatacttgg ttgttcgaac
    28501 atccagtcca ggcagctgat tttccaaaag caattcgtgc atggacgatt aacgatgaag
    28561 aagaaatggc caaatgtcaa gaactcaaaa tagatggaat aataactgat tttccggaga
    28621 aagctcttcg gtggaaacaa tcaacgaaaa gtaggtaaaa tatggaaaaa gtattagtga
    28681 tcgtcggtcc gacagcagtc ggtaaaacag cgctaagtgt ggaactcgca caactatttg
    28741 gaggagagat catcagtggt gattcattgc aaatctataa aaatctggat atcggtacag
    28801 caaaaatcaa agaagaagag atgaataatg ttccgcatca tttgattgat gtgatcgaac
    28861 ctactcaacg ttactcagcc gttgattttc agcattcagg tcgacaattg attaccgaga
    28921 ttgcgaaccg agggcaccta cctatcatag ctggtggtac gggtctctat atccaatccc
    28981 ttctatatga ttatcagtta ggagcgacag aagaagcgaa tcctgagatt agaaaaaagt
    29041 atgaaacgtt agctgaaaca attggttccc aagcattatg gaatcagttg aaagaaaaag
    29101 acccggcagc tgctgagaaa atccactgga acaatcaacg caaagtgata agagctctgg
    29161 aagttttaga gacgacagga catagtatca cggcacctaa agaagagcca aagcaattgt
    29221 atgattacct catgattgga ttagatacga agcgtagtat tctttatgaa cgaatcaatc
    29281 aaagagtcga tttgatgcta gcagaaggtt tagtagcaga agcgcgtaag gtgtatgagc
    29341 ttggagaagt ccaggccagt caaggtattg gatataaaga atttttccct tattttgaag
    29401 ggaaagaaac attggaagaa gcaacagaac aagtcaaact acattcgaga agatacgcga
    29461 aacgtcagtt gacgtggttt cgtaaccgtt tggatgcaca gtggtttgat cttataagtg
    29521 agccaaatca aaaaagtcag attgagcaaa caatcaaaaa atggctggag gcggaataaa
    29581 atggaagaaa aagtgatcat cgtaggggta gaaaccgaag caaaccagcg ttacttttca
    29641 gagtcaatgg aagagctagt tcagctgacg aataccgcat caggagaagt cgtattcaca
    29701 attacacaaa agagaccaca agtggatcgg caaacgatta tcggtaaagg gaaattacaa
    29761 gaattagttc aacaagcgga tgcgcatgaa gcggatttga tcatctttaa tcatgagcta
    29821 actccgagac agagccaact gattaccgat gctgttggtc ttccggtaat tgatcgcgtg
    29881 cagcttatct tagatatttt tgcgatgcgt gctcgttcga aagaagggaa gctgcaggtt
    29941 gaacttgctc agttagaata cctgctgcct cgtttagtag ggcaagggaa aacattatct
    30001 cgtcttggcg gtgggatcgg aacaagaggg ccaggtgaaa caaagttaga aacagaccgc
    30061 cgacatatac ggaataaaat ttttgctgtt aagaaagaat tgaaagaagt agaagctcat
    30121 cgagagcgca atcgccaaaa aagaaaaaat agcgagattt ttcagattgg acttattggc
    30181 tacacgaatg ccgggaagtc aactatcttg aatctattga cacaagcgga tacttattcg
    30241 aaagatcaat tgtttgcaac gttagatcca ttgactaaga gatggcgttt tgctgaagga
    30301 tttgagatta ctgtaacgga taccgtcggg ttcatccaag acctgcctac gcaactgatc
    30361 gatgctttcc attctacatt agaagaaagt caaaatatgg atcttttgct acatgtagtc
    30421 gatgctagtt ctccagatcg tattttacag gagcagactg tcttaaaatt gatggatgaa
    30481 ctgaatatga aagaaatgcc gatactgact gtttacaata aggcagacca gatcgaccca
    30541 gcgatgttca cgcctacgct attcccaaat gtattgattt ctactcagac gaaagaaggt
    30601 aagatggcgt tgatcgaagc aatcaaacgt cagctgatgg aactaatgac cccttataca
    30661 ctttctgtgc caagcaacga aggacagaaa ttaagcgagt tgagaagaca aacaatggtt
    30721 ttggacgaag cgtacatgga agaaacaaac gaatatgaag ttaaagggtt tgcaaaaacc
    30781 aattcaaaat ggttaagaaa aaaccaataa aattttattt ttttaa
//
LOCUS       DAZKPO010000030        30723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000030 DAZKPO010000000
VERSION     DAZKPO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.030"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACG8NZ_001291"
     CDS             complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACG8NZ_001291"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0538979.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACG8NZ_001292"
     CDS             complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACG8NZ_001292"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0538980.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8110..9078
                     /locus_tag="ACG8NZ_001293"
     CDS             8110..9078
                     /locus_tag="ACG8NZ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0538981.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9096..10001
                     /locus_tag="ACG8NZ_001294"
     CDS             9096..10001
                     /locus_tag="ACG8NZ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0538982.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9998..10804
                     /locus_tag="ACG8NZ_001295"
     CDS             9998..10804
                     /locus_tag="ACG8NZ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0538983.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10961..11929
                     /locus_tag="ACG8NZ_001296"
     CDS             10961..11929
                     /locus_tag="ACG8NZ_001296"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0538984.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12246..12770)
                     /locus_tag="ACG8NZ_001297"
     CDS             complement(12246..12770)
                     /locus_tag="ACG8NZ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0538985.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13057..14754
                     /gene="scm"
                     /locus_tag="ACG8NZ_001298"
     CDS             13057..14754
                     /gene="scm"
                     /locus_tag="ACG8NZ_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0538986.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(14909..15895)
                     /locus_tag="ACG8NZ_001299"
     CDS             complement(14909..15895)
                     /locus_tag="ACG8NZ_001299"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0538987.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15918..16484)
                     /locus_tag="ACG8NZ_001300"
     CDS             complement(15918..16484)
                     /locus_tag="ACG8NZ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0538988.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16498..16914)
                     /locus_tag="ACG8NZ_001301"
     CDS             complement(16498..16914)
                     /locus_tag="ACG8NZ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0538989.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17371..19305)
                     /locus_tag="ACG8NZ_001302"
     CDS             complement(17371..19305)
                     /locus_tag="ACG8NZ_001302"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0538990.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19607..20542
                     /gene="pplA"
                     /locus_tag="ACG8NZ_001303"
     CDS             19607..20542
                     /gene="pplA"
                     /locus_tag="ACG8NZ_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0538991.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20632..21735
                     /locus_tag="ACG8NZ_001304"
     CDS             20632..21735
                     /locus_tag="ACG8NZ_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0538992.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21748..22701
                     /gene="menA"
                     /locus_tag="ACG8NZ_001305"
     CDS             21748..22701
                     /gene="menA"
                     /locus_tag="ACG8NZ_001305"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0538993.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23515..24861)
                     /gene="gor"
                     /locus_tag="ACG8NZ_001306"
     CDS             complement(23515..24861)
                     /gene="gor"
                     /locus_tag="ACG8NZ_001306"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0538994.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(24995..26248)
                     /locus_tag="ACG8NZ_001307"
     CDS             complement(24995..26248)
                     /locus_tag="ACG8NZ_001307"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0538995.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26254..27177)
                     /locus_tag="ACG8NZ_001308"
     CDS             complement(26254..27177)
                     /locus_tag="ACG8NZ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0538996.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27398..29890)
                     /locus_tag="ACG8NZ_001309"
     CDS             complement(27398..29890)
                     /locus_tag="ACG8NZ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0538997.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(29897..30364)
                     /locus_tag="ACG8NZ_001310"
     CDS             complement(29897..30364)
                     /locus_tag="ACG8NZ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0538998.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(30500..30573)
                     /locus_tag="ACG8NZ_001311"
     tRNA            complement(30500..30573)
                     /locus_tag="ACG8NZ_001311"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30537..30539),aa:Arg,seq:acg)
     gene            complement(30631..30704)
                     /locus_tag="ACG8NZ_001312"
     tRNA            complement(30631..30704)
                     /locus_tag="ACG8NZ_001312"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30668..30670),aa:Pro,seq:tgg)
BASE COUNT         8901 a         6536 c         5740 g         9546 t
ORIGIN      
        1 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
       61 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
      121 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
      181 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
      241 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
      301 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
      361 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
      421 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
      481 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
      541 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
      601 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
      661 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
      721 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
      781 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
      841 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
      901 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
      961 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
     1021 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
     1081 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
     1141 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
     1201 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
     1261 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
     1321 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
     1381 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
     1441 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
     1501 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
     1561 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
     1621 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
     1681 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
     1741 ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt
     1801 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
     1861 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
     1921 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
     1981 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
     2041 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
     2101 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
     2161 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta
     2221 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
     2281 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
     2341 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
     2401 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
     2461 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
     2521 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
     2581 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
     2641 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
     2701 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
     2761 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
     2821 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
     2881 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
     2941 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
     3001 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
     3061 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
     3121 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
     3181 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
     3241 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
     3301 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
     3361 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
     3421 cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
     3481 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
     3541 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
     3601 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
     3661 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
     3721 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
     3781 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
     3841 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
     3901 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
     3961 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
     4021 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
     4081 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
     4141 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
     4201 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
     4261 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
     4321 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
     4381 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
     4441 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
     4501 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
     4561 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
     4621 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
     4681 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
     4741 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
     4801 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
     4861 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
     4921 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
     4981 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
     5041 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
     5101 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
     5161 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
     5221 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
     5281 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt
     5341 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
     5401 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
     5461 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
     5521 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
     5581 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
     5641 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
     5701 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
     5761 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
     5821 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
     5881 tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca
     5941 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
     6001 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
     6061 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
     6121 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
     6181 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
     6241 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
     6301 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
     6361 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
     6421 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
     6481 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
     6541 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
     6601 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
     6661 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
     6721 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
     6781 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
     6841 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
     6901 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
     6961 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
     7021 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
     7081 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
     7141 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
     7201 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
     7261 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
     7321 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
     7381 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
     7441 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
     7501 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
     7561 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
     7621 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
     7681 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
     7741 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
     7801 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
     7861 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
     7921 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
     7981 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
     8041 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
     8101 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
     8161 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
     8221 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
     8281 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
     8341 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
     8401 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
     8461 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
     8521 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
     8581 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
     8641 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
     8701 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
     8761 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
     8821 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
     8881 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc
     8941 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
     9001 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
     9061 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
     9121 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
     9181 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
     9241 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
     9301 tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
     9361 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
     9421 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
     9481 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
     9541 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
     9601 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
     9661 tgatttctaa tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg
     9721 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
     9781 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
     9841 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
     9901 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
     9961 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    10021 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    10081 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    10141 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    10201 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    10261 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    10321 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    10381 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    10441 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    10501 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    10561 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    10621 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    10681 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    10741 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    10801 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    10861 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    10921 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    10981 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    11041 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    11101 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    11161 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc
    11221 tgaatctacg atgaaagatg tcaaactggc agcagctcaa ggagattctg tttcgaagtt
    11281 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    11341 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    11401 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    11461 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    11521 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    11581 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    11641 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    11701 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    11761 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    11821 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    11881 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    11941 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    12001 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    12061 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    12121 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    12181 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    12241 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    12301 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    12361 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    12421 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    12481 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    12541 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    12601 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    12661 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    12721 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    12781 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    12841 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    12901 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    12961 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    13021 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    13081 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    13141 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    13201 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    13261 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    13321 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    13381 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    13441 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    13501 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    13561 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    13621 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    13681 tttttattgg taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt
    13741 aatccaaatg atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta
    13801 cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg
    13861 aatatctctt ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa
    13921 ggaacaatcc ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt
    13981 ataggaacaa atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt
    14041 atcagcgaca atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca
    14101 agtgatagct cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt
    14161 gatagcacgg atacatcagc ttcaagcgac agtacagata ctacctctac cacaagtgat
    14221 agcacggaca catcagcttc aagtgacagt acagatacta cctctaccac aagtgatagc
    14281 acggacacgt cagcttcaag cgacagtacg gatacatcta ccacaacaag tgatagcacg
    14341 gacacgtcag cttcaagtga cagtacagat actacctcta ccacaagtga tagctcagat
    14401 tcatctacca ctacaagtga tagcacggac acgtcagctt caagtgacag tacagataca
    14461 tcctctacca caagtgatag ctcagattca tcaacttcaa gcgatagtac agacacgtcg
    14521 tctaccacaa gcgatacacg tcatgcaagt gtctccttct caaaaacaag ttcggataac
    14581 aggacgaact ttaacttaac taataaaaca agcaatgaca atggaaacaa atcaaaaaga
    14641 gcacttccta aaactggaag tcaatcgaat aattggataa cactcgcagg cgttatatta
    14701 cttgtaatcg gtttaagaat cacttttatg agttataaga aatcgaaaag gtaaaaaaat
    14761 attcaagata aaaaagagca acgtcgctaa cggacgttgc tctttatttt tgctttttat
    14821 cataaataca agaactacaa ttcaatttgt caaaaaacac gtagatttcc ttctgagaaa
    14881 tttatttacg tatcgaacct ttccctattc agtctttgcg atccaaaatc agttgggtaa
    14941 gggaaaagag attctctttt gttcgaagag aggtggcagg taatttcttt atttccttca
    15001 aagccttttc tgtgtaagag gccgctaact tcctagcttc ttccacgcct cctgtatgtt
    15061 caacgatttt gcgtacggcg tcgacatctt cttctgtcat tgccgctttc ttctctaaat
    15121 aaggtagtag ctttggtttg tttttttgca aagagtagat caacggtaaa gaatagaccc
    15181 cttgacgcat gtcttctagt acaggtttgc cgatttcctg gctctctttt gtataatcta
    15241 aaatatcatc gataatctga aaggccatcc cgatagaaag tccgatatct cctgcttttt
    15301 ttgcgaagcg ctcgcttgtt ccactttcaa aagcgccaac agaacaactt aattggaaca
    15361 gttcagctgt cttacctgaa atattttcca aatattcttt tacagaaagt tcataattat
    15421 agcgatcatc catctgccct aattcgccat tcaatacttt ttccatactg cgagaattca
    15481 attggagact tttcaaggaa gtggagtaat cagacaatag tttaaaacag cagacaaata
    15541 aatagtctcc tgcatagaca gaaacgacat taccaaactg actacggata ttaggctgac
    15601 cacgtcgcag atccgcttca tcaacgatat catcatggat cagtgtggcc gtatggagca
    15661 tttcgataga agctgccaaa gcgatggctt tgtctcgatc ttgctctggt ccaaattgtg
    15721 agaacaatag ctgataggca ggacgcaaca gctttccgcc ggcatggatc gtttctttga
    15781 ttgcttgttc gacaggtttg tttttcaggc ggacagtttc ttccatccgt tttagtgttt
    15841 ctgtcaattc tttacttaaa gcaggatagt tttcccacat tggatgtagc ttcataaaaa
    15901 aactccttgt gtgacgatta ttttttggta aacattgggc tccatttttg tttggttccc
    15961 tgctcataat ctttttggaa tcgttgaagt gtctgcacat gtcgtaaaag ataattcgct
    16021 gcgatcccga ttgcaatccc tgataaaatc cctaaaactg aaaggactgg caagtacaac
    16081 atgactgtcc atgattgtgc aatgaaagaa gccatcaaaa gctgtccgac attatgcatg
    16141 aaaccgccgg ccgcactaat tccgatgata ctgacacgtt ttggtcctaa ttgcttgacc
    16201 aataacattc caaaataact aagcaaagca ccactcatac tataaaggaa tgtggaaatc
    16261 gttccgccta gcaaagtagt caggaatagt cgtagccaaa gtagtaagaa gctgtctcgt
    16321 tttttcatgg taaaaatggc aacgatcgtg attagattgg ctaagcctag ttttgcacca
    16381 ggcgcaaaag aaaaaggata agggatcata ttttccaata aaccaatgat cactccttgt
    16441 gcaacaagta gcgaaatata aatcattttt tgtaatttac tcatggtgtg tgaaggtcta
    16501 ataaatcaga ctgtcttcgt cacctccatc tgttgaacgg acttcgatca ctaatttatg
    16561 tggcaaacag acgatggttt cgccattttt cgttgcccag cctcgacgta cgcagatctg
    16621 gtcatcgcaa tctgcttctt ttatccgtac tcgatcgcca tctatctcga tcaagttata
    16681 atctccgtgt tcatctttgt aagtatatgt gttgctctct tctccagcca tcaaagggaa
    16741 tgttttgatt tctgtgccat ccacacgcag taccgcttct ttttctgctg ttgcggtgtt
    16801 ttgctgaaga ccaaaaatca tgactggtaa aaaagaaaga acagtcaaaa gcaaaataat
    16861 aaaaacatcc caaggtttga gcagactttt ttcagcaaat tctttaaggc tcatttttgt
    16921 tctccttttt ccagattaac tcacccaatt ttagcataaa aacaagaggt tgtgacacct
    16981 gtgtcttgct ttgagtaata agaacgaaga atagaaaaca gctttccatg ttttctaatt
    17041 ctttgggctt attaccgaga agcagcaggt ggaacaccgt ttataacaag aggttgtgac
    17101 agaagtgggc aacttcaaga aataagaagg aatttacgaa aattactttt caagtttttg
    17161 tgaaatttcg gcttattttt cgaagaagct acttctggaa caccgtttat aacaagaggt
    17221 tgtaacagag gttgtgacac ctgtgtcttg ctcatggagg cttatctcgg gcatcttatt
    17281 cctttaattt tacataaaaa ggaggctgtg acggtttgtc tcagcccctt tctattgaaa
    17341 tcttgcattc aaataacgtt ctttatctat ttatttcttg ccgtactggg aacttggtct
    17401 accgtaccac caattaccta gtgtacgttg tagccatgga attacccagc gatccaaccc
    17461 aatcgcacgt ccggaaccgt tcatcaatgc gaaagcaaca aaaatgaacc aaatgtttac
    17521 ccagtaaaac atgcctgata agcagaacgt gatcaccaat acaatcgttg tcgcactgca
    17581 tagccatgtg aataatccag caatcaatgc taatgccaag gcaatttcaa agaataccat
    17641 gaatttttgg aagaacattg ctacatcatt gtttggcatg atcacttgca tgatctttgt
    17701 gaaccagtct ggtgacttct caatcacagc cattggttct tcaccgtatg cataactcaa
    17761 accaaaatgt gcagcttgag tcgctgcttc tccggcactt gcccctgctc cgctggcagc
    17821 tgtggttgca tcaccagcag cagaagcacc actggcaaca tctgttacat tgtattccca
    17881 tgtccaaggg aacacactgt ttccagtgaa ccatgatcct tcaccaaacc aagtacttgg
    17941 tttgaacact tcaccattcc caatgatttt tttggaagct tcaacgaacc aaactaaacc
    18001 atagaaaata cgaagtggca cactccataa gacattgccg taacgagaag aatgtcctcg
    18061 agtcacgttg cgatcatctt tgatatggaa aaactcatgc attaaatatt ggaacatata
    18121 ataaccagaa cgaatatcaa agaagtattt caaattcacg atatgtttca ttacgatagc
    18181 taaaaatccg cttaaatgga tcttatcaaa taggttagct accccccatt tggcaccaat
    18241 agaaaccata aatccttgat agtttccttt aaatttatgc ttttctccgc ctttgatcga
    18301 agccacgata ttcgctgcgg ctgtgtgtcc tgtttgttca gcagcttgaa cgatctgcgg
    18361 tgtcggtgtt cctgggaatt cttcataaaa gactaaatca ccaatgatat agatgttttt
    18421 atcttcgtag ccttttgcct gcatatattc attggcgatc aaccgctgtc cgcgtgctga
    18481 ttctaatcca aagtcagcag cgtcagaagt tcctttgaca ccagctgtcc aaatcagtgt
    18541 gtgagtcgga atgttgcgtc cgtctttcaa tttgatatga tcagcagcca cttctacgat
    18601 tggtgcattc aataataatt ccacgttgtg cttacgcaaa tagcgttcag cttttgcagc
    18661 atcatttcga tccaacatat tcaagatcgt cggcattgcc tctacgacca tcagtgtgat
    18721 ttcgtttgga tctaatttat aatctttggc taggcgattt ttccagtcga tcagttcccc
    18781 aatcatttca ataccggtaa atccagaacc acagacaacg aaagtcagca tggctttacg
    18841 cagttcatca tctggttcca atgcagcttt tgctactgtc ttctcaatat gttcccgaat
    18901 ccttacagaa tcttcaaatg accaaagggt gaatccgtgt gctttgacac caggtgtacc
    18961 aaagtcattc ggctctccgc ccattccaag gatcagctga tcaaaaggat aggaacctaa
    19021 tttggtcttg acgatttttc gttctttatc gataccgatt acagtgtctg tgacaagttg
    19081 tacattcttt tgacgagcaa acaaatgctg cagatcgtat tggattgctt ctggttctac
    19141 acgtccgcca gctacttcat gcaactcggt catcatagta tggtacgaat gacgatcgat
    19201 cagagtaatt gctacatctt cttttttcag tttctttgcc aagtattttg tagctgaaac
    19261 accggcatat cctgctccta caacgacaat attttgcttt gccattgatc atccgctcct
    19321 tatagactat ctatataatg aatatatgat aattctttta gtttgatact tattacacaa
    19381 tctccatata ttatatagat gttctgttct tttgtctaac tccttttgga tttttttcaa
    19441 attttaaaat aaagcgtttt ttcacgaaat ttgctgaaag aaaacgtttg aaatattaca
    19501 tgggttattt atcaaaaata gttgacttat aggctaaaaa gattagaatg tataatgaat
    19561 cacaaagaaa aattgtggac tagaaatgga atggtggggt attaaaatga agaaaaaacg
    19621 ttttgtaact ggatttgccg tattagcatt ttcagctcta gtgttaggcg cttgcggcgc
    19681 agatgacaac ggaagctcaa actcttcttc tgaatcatct acagcacaat cttcgactgc
    19741 aaaaagcaca acagaatcat ctgcaaaagt tgtagctggc ggagatttgc aagacggcac
    19801 atataaatta gaagaaaaaa attattcaaa tggttaccgt gcagtctttg aaatggttgt
    19861 aaaagacggc aaaatcactg aatcaaaata tgataacgtc aatgaaaacg gcgaatcaaa
    19921 aacaaaagat gcagaataca acaaaaacat ggaagcaaaa tctggcacaa acccagaaaa
    19981 atacattcct gagttgaatg accaattctt aaaagcacaa agcgcaagcg gtgtggaagt
    20041 cgtaactgga gctactcact catctgaaag cttccaaaac tacgcacaac aattgatcca
    20101 agctgctcaa gctggtaaca cagatacaat cgaaatcgac aatggtgctg atttgaaaga
    20161 cggcacttac aagcttgaag aaaagaacta ctctaacggc taccgtgtgc aattcgaaat
    20221 gacagttgct ggcggcaaag taactgaatc aaactttgat tatatcgaca aagacggtaa
    20281 atcaaaacaa gatgatacag aatataatga aaatatgaaa gcaaaaagcg gtacagaacc
    20341 aaaaacttac atccctactc tgaacgacga attcgtcaaa gcaatgggcg aagaagatgg
    20401 ctcacctgct aatgtagaag ttgttacagg tgctacacac agctcacatt cattcatcat
    20461 gtacgcacaa caattagtga atgcagctga aaaaggcgac actcaaacaa tcgaagtaga
    20521 taatatcgta acagaaaaat aatcacataa tcatactaat aaaagttgga gggtgggacg
    20581 atagccagat taggcagtcg tctcaccttt caaaatgaag agaggaaaaa gatgcggaaa
    20641 aaactactat tttccttaat cacactgggc atctctttta tggtgctaac tggctgcagc
    20701 aactcagcaa ataaaacaac cactgaaagt tcagcagcta ccaagatatt gaaagatcca
    20761 tattctgatg aacagttctt gttagggact tacgttcgta tccgtgtgta tgatgaaggc
    20821 aaagaatcag cattgaaacc tgccttcgat cgagtaaaag aacttggtga taaaatcacg
    20881 atcaatcaaa aaggttctga gatcgatgaa gtgaatgagc aagctgggat caaaccagta
    20941 aaagtttctg atgacgtcta cacgttagtc aagcgggcct acgaatatag ccaagattct
    21001 caaggcggat tcgatatggc gattggtgca atcacacaat tatggcgaat cggttttgat
    21061 gacgccagaa aaccttccca agaagaaatc gatcaggcat tgaaattagt ggattatcat
    21121 aagatcgaac taaatgacaa agaaaaaact gtttatctaa aagaaaaagg aatgatcatc
    21181 gatttaggtg caatcgccaa aggttacatt acggacgaag tagtgaaagt cttgaaaaaa
    21241 caaggagtga ccactgccat cgttgacttg ggcggtaatg tctatgtctt aggccacagc
    21301 ccaagaggcg aaaaccaaga ctggacagtc ggcatccaag atccgaacat ggctcgcgga
    21361 tctgttttag gttctattaa agaacggaac aaaacattag ttacctctgg aatttacgag
    21421 cgttatttag aagtagatgg caaaaaatac catcatttgt ttgatccaaa aacgggttat
    21481 ccgttcgaca atgatatcgc aagtgtcacg atcataacag ataaatcaat tgatggtgac
    21541 gggttgtcta cagcagtctt ttcaatgggt gtgaaaaaag gactggaata cgtcgaaagc
    21601 gaattgaata acgggacgga agcgatcttc gtgacaaaag acgataaagt atatgtgacc
    21661 gatccaatca aagacacctt caaattatcg gaggactccc attacacgat gggtgaccgt
    21721 tctgaactaa aataaggagt acgaaccatg acactaaaaa tctttttaga agtagttgaa
    21781 atacgaacaa aggtggctag tgtatttcct tttgcggtag gggtattatt ctctatcgct
    21841 ttttttcatg agatacattg gctaaacact tttttatttt ttattggaat gctagttttt
    21901 gatttagcga ctaccgctat caataattac atggattatg aaaaggctca ttctgaagta
    21961 tataagtatg aagaaaatgt cattggccgc agaaatctat ctcctgtatt gattcgaaac
    22021 atgattttag ctatgattgc atttgttttt gttatcggcg ttattctaac gatcaatacg
    22081 ggttggcttt tacttttgat gggatttatc tgttgtttta ttggagtatt ctatacattc
    22141 ggaccgattc ctttatctcg aatgccttta ggggaaattt tcagtggggt gactatgggc
    22201 cttggaattt ttgccatggt cgtttatgtc aatacattcg gttcagatgt ctatgatctg
    22261 acagtcaact tttcatctgg acaatttgct ttggtaggaa atctttgggc tatagcagca
    22321 atcgtattag cttccttacc cttggtgttt acgattgcca atatcatgtt ggctaataat
    22381 ttgcgggatc ttgaccgtga tattgaaaat catcgctata cactagtttt ttatatcgga
    22441 cgtcctgcgg gaacagtctt attccagcta ttgatgtatg cttgctattt cgtgatttta
    22501 atggggttgt tgagcggtat ctatgaatgg ccgatcttgg tcaccttctt gaccttgcca
    22561 attgtgaaga agcatttgga tcaattcaag caaacgttac cccagcctat cagtttcagc
    22621 tattccatca aaaatatggt cttattcaat gccagttatg tattggggct attactaaca
    22681 atcattatgc aagccattta acagcatgaa gaggttgtga aaaaactgcc ctgcatcaag
    22741 taataagaac agccaaataa aaatagctcc ttatattttt cgtttggttg ggcttattac
    22801 cgcagatgca agcttttgaa caccgtttat caggaggttg tgaaaaagtc gcttagtttc
    22861 gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa
    22921 taccgagaaa ctggcttttg aacaccgttt atcagaggtt gtaaaatcag tgttttgtcc
    22981 tgagtattag agaaacaaat ggaaaaatag ttcctcatat tttgccgttt tttgagctaa
    23041 taccgaagga cagctgattg aacactattt gttcggaaaa agtgactaac tatgacacaa
    23101 tttttttcac agtccctttt ttcacttgat ttgattcgta ccagcgaact tgacgagaac
    23161 tttcttggtt tggtaactat atggtattct tttaacatcc tcgtttgctt cttctaattt
    23221 atttatacaa agtcacactt ttttaaacat ctgttcttcg atacatttct atttctttta
    23281 aacatcgata ccaatatttt gaacttaaga gtgcatacca aatggattca tttactcttc
    23341 gtcagttacg aagataatca aaatctccaa cttccctact tatttcgttt tgagaattat
    23401 cgaagcaatc ttacataaat aactaaaaaa caaaaaacca gaaaatcagt atactgattt
    23461 tctggtttta catggtttgt tcaataaaaa gctgttggaa ctgttaatat taaattatcg
    23521 catcgttacg aattcttccg ctcctgttgg atggatggct actgtattgt caaagtcttc
    23581 tttagttgca cccatcttga tagccacggc aaaaccttgc agcatctcat cgacaccaac
    23641 accaatagca tgtaagccga cgattctttc ttcttttccg acacagatca atttcatttc
    23701 gcatttttgg cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac
    23761 tttcaattca ttttcgccat atttttccaa tgcttcttct tctgtcaaac caattgttgc
    23821 aattggtgga tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat aagaatcttt
    23881 ctttccatta aataaacgtt ctgataaacg tctaccagct gcgattgcaa caggagtcag
    23941 atctaattta ccgatcacat caccaacagc aaaaatatgt tcatccgttg tattttggta
    24001 tttatctact ttgataaacc cttttttgtc caattccaca tttgtatttt ccagacctaa
    24061 tttttcggta ttcggtacac gtccaccggc aaaaagtaca caatcaccag ttaaggtttc
    24121 accattttca aatagcaccg tatattcgtc attgttttta gtaatttctt tagcaatatg
    24181 atgtgcgtaa gtttttattc cagcttcttg gtacatttgg atcaaattgt ccgaaagcat
    24241 tttatcaaac gttctaagtg gtcgttcttt tcgatatgcc cacatgactt cactacctaa
    24301 accacgaaat acacctgaaa gttcagccgc aatgtatcca gcacctaaaa cgattgctct
    24361 cttaggtaac tctgttaatt caaaaaatcc atttgaatca attgcgtatt ctccaccagg
    24421 aatggtcatt ttacttgcac ggcctccagt agcgatcaag attttaggag cacgataggt
    24481 ttcaccgttt acttcgatcg tttgactatc gataaaagta gcgtaacctt ttatagcctc
    24541 gacatgattg ctatctaatc ctctttgata agcaccgtgt agaaaatcaa tatatttttc
    24601 tcgtctttct actagaccgg cgaaatcaac attttttaaa tctgcttgga taccgtaact
    24661 tgaggcatct cgttcaattg tttccaaaat cgtacttgct tgccacataa cttttttagg
    24721 tacacaaccg acgttgacac atgttccgcc caattcattc gcttcaatca gcaaaacttt
    24781 tgcgccatgc atccctgctc tgtttgctga agcaataccg ccagagccgc caccaattac
    24841 gatgtaatca tatatttgca ttttgttcct ccttattgaa gagatcaatc aaaaccttta
    24901 tttaacgatc ctttttctac agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga
    24961 agatgcttaa aacagggata tatcagcttt ttccttattt acgttttcgg atcatatctc
    25021 caggtttcac cggctgtgcg actggcatag ttaaaatcat cattggattt ggtgctcggt
    25081 caatggattc gccttcttca ttatgcataa cttcaacggt ttgactgaaa tgactgaatc
    25141 ctggaccata aaattcgatt tcatctccta cagaaaacaa gtttctctga cgtatcgtag
    25201 caatctgtgt tgcctcatca taagataata cttcaccgac aaatttatat actgggattt
    25261 tacgacgttc accgaataat tgttcgtttt cagttggtgt attgtagtaa aaacctgtag
    25321 ctaattctcg ttgtgctact ttccataact cgtcgatcca ttcttgcttg cagacatagt
    25381 tttcaggatc ttccatataa ctgtctacag ccgctttata gacatttgac accgtagata
    25441 cataatggat cgatttcatt cttccttcga tcttgaagct gtctacgccg ttttcaatca
    25501 attctggaat atgttcgatc attgacatat ccactgcact catcgagaat tcttcttcca
    25561 cttcgcctgt atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat
    25621 acttccagcg acaagattga gaacatccgc cacggtttgc atcccgcatc gacatatggt
    25681 tggacaatgt gcatcgacca gaataagaga tacacatggc accatggata aatgcttcga
    25741 tttcggtatc cgtattttta cgaatctctg ccacttcctc cattgaaact tcacgagcta
    25801 aaacgacacg ttccaaccct tcttctttcc agaattccaa tgtctcaaaa ttagttgctg
    25861 aagcttgagt ggataaatgg atcggcagac ctggggcttc tgtcgcacaa atttcgatca
    25921 aagctggatc agaaacaatc actgcagaaa taccgacatc acgcaaactg cggaaaaatt
    25981 cccctgctcc atcttcattt ccttcatggg tgaccatatt agcagcaacg tataccttcg
    26041 catcatgttc ttttgcaaaa gcaactcctt cagccatttc ttcataagta aaattacctg
    26101 cacggcttct taaaccatat gcattcccgc caatatatac tgcatctgct ccataatgga
    26161 tcgcggtttt taacttttcc agtgttcctg ctggcgctag gatttctggt cgtttcaatt
    26221 ttctttttac tgtcatttct ttcatcatac tccttatttg atagcagaag gatcaatata
    26281 aaagaatcct gtatctaatt cacggttttc aggatgcaat ttactgattt cgctagtcat
    26341 tttttccgct ttttcttgat cccagctacc ttctaaaatt tgtttttttg cttcggcgaa
    26401 tagtcgagtg atgtttacga atttttcgcc tggagtaaat aatccgtcca atttccatgt
    26461 acagtaatga tgctgataaa gttcattcaa ttcattcgag agattcagat cattgctggc
    26521 aaaaatatgt gtgccatgac tatcttcata aatagaataa tgtgtctctt cttttttagg
    26581 ttccgagata aaaagacctc tttcacgatc tttttgttcg tcttgtttcg tatagttata
    26641 gtaattctgt agcagtggac gtttggattg atggatgcat gtagcaccat acacaagggt
    26701 ttctacagga atatcgagtt tttcttccat cgccttcatc tcttcaaaag gaacttcacg
    26761 agcaagtact gctccaactg cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt
    26821 tactaatgtt tcagcatcat aaatatacgg tatacgcaat tcaggatttt ttttcatcgt
    26881 ataaataata cctgtatcac caactgtgat cacatctacg tttatttctt ttaaaaaagc
    26941 caaatattca ggtacatttt tcattttttc aggatgaatg atcccattta cagcaatatt
    27001 cgctttttta ccatacttgt gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa
    27061 tgaagcaggc agacgcaatc caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc
    27121 agcaagcaac tgttctgctt gttcaatagt ttctactgtg gttatgagtt cgatcataat
    27181 tttctcctcc tacaacttca taatagtagc atagctctaa aaactttcca atacttcttc
    27241 tatttaaagt cggatttttg aaaattattt cacaaaaaat caaaataaag ttcgatatat
    27301 tctgtccaaa tataaaaaga gattgtgaca aaaatcttgt cacaacctct taatcaagtc
    27361 ttactattat aaaaattcag ctacacaggc caagtaacta tagggaacct actgtttcct
    27421 ttttaggtcc gcgaacattc aatgtgatct taccttttga agcacctact gttacgtggt
    27481 cacctaaacg aatttctccg ccaagcaaag cctcgctcaa tcgatcttca atttcttttt
    27541 gaagtgcgcg acggattgga cgtgcaccat attcaggatc aaatccagct ttcccgatca
    27601 catcgattgc agatggtgtg attttcactt gaatatcttg ttctttcaat cgtttgataa
    27661 tcgctttgct catgatttgg acgattgtat ggatttcatc tttactcaat gaatggaaga
    27721 cgacagtctc atcgattcga ttcaagaatt caggtcggaa agctttcttt aactcttcta
    27781 agatacgttt ttgcatggct ttgtgatctt tagtaacatc ttggacattg aatccgacat
    27841 ttttttcttc tcgaatttgt gtcgctccga tatttgaagt cataatcatg attgtatttc
    27901 taaagtccac ttttctacct ttagaatctg ttaaatgacc atcatccaat acttgcagaa
    27961 gtaaattgaa aacgtctgga tgtgctttct ccacttcatc taagagaatc actgaatatg
    28021 gtttttgacg aattttttct gtcaactgtc caccttcatc ataccctacg tatcctggag
    28081 gagacccgat taatcggctt gtactgtatt tttccataaa ttctgacata tcaacacgga
    28141 tcagggcatc ttcacttcca aacatgactt ctgaaagtgc tttggccaat tctgtttttc
    28201 cgacaccagt tggtccaagg aacatgaatg aaccaattgg acgatcagga tctttcaatc
    28261 cgcttcgtgc acggcggatc gcacgtgaaa ctgctttgac agcctcatct tgaccaacga
    28321 cacgttcatg cagaagtcct tccagttcta acaagcgttc gctttctttt ttctccaact
    28381 gctgtagagg gacaccagtc cactccgata caactgtagc aacatcttct tctgttacac
    28441 gatcagaata accttgctcc tgttttgctt caattgcaga aactttttgc agctttttac
    28501 gtagtgcttt ttcttgtctg cgtaattgag cagcattttc aaagtcttgt ttttggatcg
    28561 ctgcttcctt ttcttgtact agttcatcga tttctagttt gatcacagtt gattttgtta
    28621 agtgatcggt ttgatccaaa cggactttcg ctgctgattc atcgatcaaa tcaattgctt
    28681 tatcgggtag ttgacgagaa gtgatataac gaacagaaag attgactgct gctcgtacag
    28741 cttcatctgt gatctccact ccatggtgct cttcatatct tgtacgtaat ccttgaagaa
    28801 tcacttctgc ttcttcagga gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta
    28861 atgcagaatc tttttcgata tatttttgat attcatctag ggtcgttgct ccgatcgttt
    28921 gtaattctcc tcgagctaac gccggtttca agatattcga agcatcaatt gctccttctg
    28981 ctccacctgc accaattaag gtatgcaatt catcaatgaa taagattact tgaccatcat
    29041 tatagatttc gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga tatttcgtac
    29101 cagcaaccaa agatcccata tcaagcatca tcaggcgctt ttcttgcatg tcttctggca
    29161 cttcacctaa aatgattttt tgagctaacc cttcagcaat ggctgttttg cctacaccag
    29221 gttcacccac cagtacaggg ttattctttg tccgtcggct aagaatctgg ataagccgtt
    29281 tcacttctct cgaacgacca acaactgggt ccagcttgtt ttcttttgct aatttcgtca
    29341 aatcacgtgc taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta
    29401 catttacatt tctacgacga ttcattcctt ttccagcaga atcaccagct agccccattt
    29461 tttgtttcag aacttgacgg gcttttgaaa ggctgatacc taagttgatc atgattcgag
    29521 tagccatgat actttcatct tttaataagc tcatcaaaat atgttctgtt ccaacttttt
    29581 gagaaccagt tttcttcact tcgctgctag ctaaagcaaa aacttgtttc attcttggtg
    29641 aatatggaag aaagactcct ttagggtaag aacggatacc accataacca atcagatgtt
    29701 caatttcttc ataaatttcc gtttcattca aatccatttc acgtaatact tttccagcaa
    29761 ttccattttg ctcgaccact aaggctagta aaacatgttc tgaactaact gattgatgtt
    29821 tcaaccgttt tgcttcttct tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa
    29881 gttcttccat ctaatctcac ttcccatctt cataacttaa acgttccaga aatgctcgga
    29941 gaatccgagc acgtgtatga tcttcatatt cattgaaatt caatgtgttt tttgcaattg
    30001 cactcaacat caagtttcct tctttttttg taattatttg gtcttcataa agtttttgaa
    30061 taatagcaaa agcatttttc tcactgattg tatcatcaaa taactgattt atctgctcaa
    30121 gtaattgttt tttatctgaa atccttactt ttgcaattcg tatataacca ccaccaccac
    30181 gtttgctttc tactagataa ccacgttgga tggtaaaacg ggtattaatc acatagttga
    30241 tttgtgaagg aacacagtta aagaggtctg ccatttcaat ccttcgaatc tcgatcattt
    30301 cactattttc cagaattttc tttatatatg attcaattaa atccgaggta ttctgatgtg
    30361 ccatcttatt cattcccttt gactaatatt gaccttaatt ataacgaatt tgataataga
    30421 aagaaagaag aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga
    30481 atatactatt ccgtatcgac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
    30541 gtcaaacact ctatccagtt gagctatggg cgcatattca atttaaaaaa attataaatc
    30601 atttgaaaac aaaaagcaag ataaaaaaaa tcgggaagac aggattcgaa cctgcgaccc
    30661 cttggtccca aaccaagtgc tctaccaagc tgagctactt cccgtttaaa aaataaaaaa
    30721 gac
//
LOCUS       DAZKPO010000031        29397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000031 DAZKPO010000000
VERSION     DAZKPO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.031"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(424..1368)
                     /locus_tag="ACG8NZ_001313"
     CDS             complement(424..1368)
                     /locus_tag="ACG8NZ_001313"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0538999.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="ACG8NZ_001314"
     CDS             complement(1664..2608)
                     /locus_tag="ACG8NZ_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0539000.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2828..4042
                     /locus_tag="ACG8NZ_001315"
     CDS             2828..4042
                     /locus_tag="ACG8NZ_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0539001.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4064..6079
                     /locus_tag="ACG8NZ_001316"
     CDS             4064..6079
                     /locus_tag="ACG8NZ_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0539002.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6162..7535)
                     /locus_tag="ACG8NZ_001317"
     CDS             complement(6162..7535)
                     /locus_tag="ACG8NZ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0539003.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7561..7845)
                     /locus_tag="ACG8NZ_001318"
     CDS             complement(7561..7845)
                     /locus_tag="ACG8NZ_001318"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0539004.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7847..9862)
                     /locus_tag="ACG8NZ_001319"
     CDS             complement(7847..9862)
                     /locus_tag="ACG8NZ_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0539005.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10041..10346)
                     /locus_tag="ACG8NZ_001320"
     CDS             complement(10041..10346)
                     /locus_tag="ACG8NZ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0539006.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10435..11465)
                     /locus_tag="ACG8NZ_001321"
                     /pseudo
     CDS             complement(10435..11465)
                     /locus_tag="ACG8NZ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11672..12547
                     /locus_tag="ACG8NZ_001322"
     CDS             11672..12547
                     /locus_tag="ACG8NZ_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0539007.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12699..13541)
                     /locus_tag="ACG8NZ_001323"
     CDS             complement(12699..13541)
                     /locus_tag="ACG8NZ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0539008.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13633..15510)
                     /locus_tag="ACG8NZ_001324"
     CDS             complement(13633..15510)
                     /locus_tag="ACG8NZ_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0539009.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15507..16928)
                     /locus_tag="ACG8NZ_001325"
     CDS             complement(15507..16928)
                     /locus_tag="ACG8NZ_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0539010.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16931..20320)
                     /locus_tag="ACG8NZ_001326"
     CDS             complement(16931..20320)
                     /locus_tag="ACG8NZ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0539011.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20577..21968
                     /locus_tag="ACG8NZ_001327"
     CDS             20577..21968
                     /locus_tag="ACG8NZ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539012.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22097..23371)
                     /locus_tag="ACG8NZ_001328"
     CDS             complement(22097..23371)
                     /locus_tag="ACG8NZ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0539013.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(23550..24197)
                     /locus_tag="ACG8NZ_001329"
     CDS             complement(23550..24197)
                     /locus_tag="ACG8NZ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0539014.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(24205..24891)
                     /locus_tag="ACG8NZ_001330"
     CDS             complement(24205..24891)
                     /locus_tag="ACG8NZ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539015.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24897..25970)
                     /locus_tag="ACG8NZ_001331"
     CDS             complement(24897..25970)
                     /locus_tag="ACG8NZ_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539016.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            26192..26866
                     /locus_tag="ACG8NZ_001332"
     CDS             26192..26866
                     /locus_tag="ACG8NZ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0539017.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26871..28211
                     /locus_tag="ACG8NZ_001333"
     CDS             26871..28211
                     /locus_tag="ACG8NZ_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0539018.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(28360..29346)
                     /locus_tag="ACG8NZ_001334"
     CDS             complement(28360..29346)
                     /locus_tag="ACG8NZ_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0539019.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8570 a         5556 c         5363 g         9908 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctattta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaatgtctaa ttcagctagt tccaattgac
     2641 tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact gctattatat
     2701 gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac ttcctgtgat
     2761 tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt acaaaaatga
     2821 aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag tctctcttat
     2881 tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta cttatgccac
     2941 gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg tcactatttt
     3001 tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg tgatcatcgg
     3061 attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt acagtattgt
     3121 cttagcttca agagctggac taggtattgg ctttggattg atcaattctt tggcagtcag
     3181 catgattgct gccttttatc acggagatga acgagcctct atgattggtt ttcaaagtgc
     3241 atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag tacagttcgg
     3301 ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct tgtttgtttt
     3361 gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc caacaagaca
     3421 agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc tggtcatcgt
     3481 ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt ctctaggaaa
     3541 cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg ctgccggagt
     3601 ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt ctttactact
     3661 gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga ttgggtttgg
     3721 tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca accaagtaag
     3781 tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg ttggcgctaa
     3841 tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt ctcttgggag
     3901 taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg cgcttattag
     3961 ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg aagaaataaa
     4021 agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa atgaaaaaca
     4081 taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac tgctacaaaa
     4141 tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg atgattgcca
     4201 tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt ttaaagtgac
     4261 gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg gaaaatggct
     4321 gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat cagggctacc
     4381 agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag caagtaattt
     4441 tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa aagatccaaa
     4501 tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga ttggcggagg
     4561 aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat ctctggatga
     4621 ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta tggatatcga
     4681 ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta aacgcgcttt
     4741 tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag tttttcatga
     4801 atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac tgaatcagat
     4861 ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta tcgacggtat
     4921 caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga tcaatcaata
     4981 tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag gacaaaatga
     5041 aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga ttggtatcga
     5101 aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc ctaagatgca
     5161 atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt atgggagagc
     5221 aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc ttgctgcaga
     5281 acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata tcttagctga
     5341 aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat atcaactatt
     5401 gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta tcgattgcta
     5461 tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt tattcgattt
     5521 tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg acactatcac
     5581 acagattact actccatatt tcaaattcta tgaacaagat gctttatgca aactcggaca
     5641 gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat ctcatgatgc
     5701 ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt acagtatgta
     5761 tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa tctatctttt
     5821 aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat ttatgataca
     5881 acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag atggaaagat
     5941 ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag gcggaacagt
     6001 gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct taacaagcta
     6061 ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga ctttaaatct
     6121 tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg ttctttagaa
     6181 acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag taaaaagaaa
     6241 gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc aagtacaagt
     6301 cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag atctcctaat
     6361 actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc agaacctaat
     6421 accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc tccgcagaag
     6481 aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat agcaaacatg
     6541 gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt aggtgcatat
     6601 ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt tttagcaatc
     6661 ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag gacgataaca
     6721 aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt gtctccattg
     6781 cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac atatacgatc
     6841 gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact tttaggaatc
     6901 tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca tccactagaa
     6961 gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt gatgttcttc
     7021 ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa gaccccgcct
     7081 aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac tgctgttaga
     7141 caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct tgtgaatcta
     7201 gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg tgtaccgtat
     7261 tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg agtatgcaga
     7321 gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc agcaccgccg
     7381 atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc tgaaaatttc
     7441 ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga gggctgactg
     7501 acgattgaca caaacaaatt caacaattta tccattgata gttcctccct tgaatcattt
     7561 ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt caatgatcga
     7621 ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca aagaatgctc
     7681 aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat gacttacttc
     7741 aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca tctctaccat
     7801 aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat tcgtctcctt
     7861 ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct tgaagatttt
     7921 gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc tcattatcga
     7981 tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc agatccacag
     8041 gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct ggacgagcat
     8101 gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc acgcgctcga
     8161 tcatagcttt gatataactt tcttctattt tattttctga taatagcggc tgtgcggcaa
     8221 atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt tcttctgtta
     8281 ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg ttcattttac
     8341 gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg atataaggca
     8401 gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc agccaatctc
     8461 tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga tgcagaacat
     8521 agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata atatcgtaag
     8581 aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa aagatcatcg
     8641 ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca cttggacaga
     8701 cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg atctctccgc
     8761 caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct attcgtcttt
     8821 ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga tgcttaattt
     8881 gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag gcagggacca
     8941 agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg gccatcagga
     9001 attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt gcaggctcct
     9061 ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc tccgaatttt
     9121 gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg actgcaagag
     9181 aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt cgaatggttg
     9241 tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga agatctgata
     9301 gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg gaaagggttg
     9361 cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga gcagtgcgat
     9421 tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca gttttcctag
     9481 tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg atatctgaca
     9541 ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa acagacgttg
     9601 cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta tacacagctt
     9661 caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat ttctttatac
     9721 ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc gcctcgcttg
     9781 tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt gttttcagga
     9841 gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct agtatggtag
     9901 cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt acgactgtac
     9961 cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt ctcctgcttt
    10021 tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt cagttcttca
    10081 aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg attgggctca
    10141 ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc tttattttct
    10201 gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc accagaatta
    10261 gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct taatcgtttg
    10321 tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat actttttcat
    10381 cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa tatgtcatat
    10441 ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca tttgtaagat
    10501 tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt aaatcccttc
    10561 gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat aactcccttg
    10621 ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa agtcttcatg
    10681 atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt gtttcacgaa
    10741 catccctttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat
    10801 tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt
    10861 cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa
    10921 agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat
    10981 tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa
    11041 atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac
    11101 cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct
    11161 tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg
    11221 agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg
    11281 atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt
    11341 ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt
    11401 cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta
    11461 agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag
    11521 tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggcttg
    11581 gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt
    11641 tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca
    11701 agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc
    11761 tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat
    11821 cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg
    11881 gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt
    11941 ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc
    12001 aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt
    12061 gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa
    12121 attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga
    12181 ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag
    12241 cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac
    12301 cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt
    12361 tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt
    12421 cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat
    12481 ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa
    12541 aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg
    12601 atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg
    12661 aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg
    12721 aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat
    12781 ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt
    12841 gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt
    12901 gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca
    12961 gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg
    13021 atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc
    13081 agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt
    13141 ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc
    13201 aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt
    13261 ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct
    13321 tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct
    13381 tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta
    13441 ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa
    13501 agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg
    13561 actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt
    13621 cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag
    13681 tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag
    13741 tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata
    13801 agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc
    13861 tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt
    13921 acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt
    13981 ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc
    14041 tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc
    14101 cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt
    14161 tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac
    14221 gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt
    14281 gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc
    14341 atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg
    14401 agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc
    14461 taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc
    14521 attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac
    14581 tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc
    14641 cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac
    14701 gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga
    14761 atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc
    14821 tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa
    14881 ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc
    14941 atcattaccg actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta
    15001 tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt
    15061 tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc
    15121 attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc
    15181 aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc
    15241 tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc
    15301 taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc
    15361 gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa
    15421 cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa
    15481 cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac
    15541 aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt
    15601 tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata
    15661 ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca
    15721 cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac
    15781 ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc
    15841 tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata
    15901 aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc
    15961 ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt
    16021 ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa
    16081 atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat
    16141 ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc
    16201 gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt
    16261 tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca
    16321 atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg
    16381 ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt
    16441 acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg tactttggat
    16501 tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa
    16561 gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa
    16621 gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt
    16681 agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga
    16741 tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg
    16801 tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct
    16861 acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt
    16921 tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg
    16981 cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta
    17041 atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg
    17101 acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta
    17161 tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat
    17221 aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg
    17281 attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt
    17341 tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg
    17401 tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat
    17461 cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa
    17521 cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg
    17581 tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg
    17641 ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag
    17701 gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg
    17761 tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt
    17821 tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt
    17881 gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat
    17941 caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg
    18001 aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg
    18061 catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga
    18121 atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc
    18181 tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg
    18241 gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt
    18301 gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata
    18361 actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta
    18421 attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat
    18481 ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt
    18541 ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg
    18601 gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa
    18661 tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta
    18721 cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa
    18781 gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg
    18841 agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc
    18901 gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg
    18961 atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc
    19021 gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc
    19081 gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat
    19141 gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac
    19201 caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac
    19261 ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca
    19321 tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa
    19381 tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata
    19441 cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa
    19501 acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac
    19561 cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat
    19621 ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat
    19681 ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg
    19741 taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa
    19801 atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg
    19861 gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat
    19921 tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca
    19981 gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct
    20041 gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac
    20101 caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg
    20161 ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat
    20221 catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca
    20281 gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt
    20341 ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta
    20401 tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca
    20461 taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt cattgaaaga
    20521 aaagggttga gggagaaaaa catatgatca ggttgttgga attaaactat agaaagatgg
    20581 aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa gaacttgcca
    20641 aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc aaaatccgct
    20701 ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt gaacatttga
    20761 atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca aacagtcgtt
    20821 tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt gaaaaaattt
    20881 ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt ttagaaagct
    20941 atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa agtgagctcc
    21001 agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc acgttgccat
    21061 ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt cagatgaatt
    21121 tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt gttgtgcatc
    21181 tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct tatgtacagt
    21241 tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga aaattgaagt
    21301 acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca gaattttcgg
    21361 agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt
    21421 catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta acgcaaattt
    21481 attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt gcaacttact
    21541 ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt gcagaacttc
    21601 aattttattc cactctactg aatcagaatc tgatttatta tttatctagc tatatctttt
    21661 ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa atactgatta
    21721 tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt gatattcttg
    21781 ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg tcgaaataca
    21841 atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc catgttcctc
    21901 atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc cgcttattcg
    21961 agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat ggctcttcgt
    22021 caaattgtgt tggcgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg
    22081 tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc
    22141 gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat
    22201 tcagcccctc agtgacgccg tttgagtggg gcaccttaaa ggcatttgaa atacccgatt
    22261 ggtagcgatg gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg
    22321 ttttaagggt ttcttgaaag ccttccgtat ctcgtttaac gaaataatag cgtaataatt
    22381 gacaaaaatg atacgcagtt cgcaattctt cgttgtagct taacagctca tcaacgattt
    22441 ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagt ggctccaatg
    22501 tcgtggaatc tttgagtaag agtttccaat atcgcttgag ccgacggtac ttcttctgat
    22561 tttcagagtg tgaggttcga aaacgattca tgatctggac acgtaattga ttgaagctgc
    22621 gattgatgtg ttggacaata tgaaagcggt cagttacgat ctccgcattt ggaaagactg
    22681 ttttaagcaa ctgacaatag ctggcattca tgtccatcac taggtacttg acctttaaac
    22741 gggcctttct aggaaagcgc aggaagtatt gagttagttt gtaaagtctg cgatcttcaa
    22801 ggatcgtaaa tagctgattg gttgtcccat caacacaaat aaaactcatg gaaccagaaa
    22861 cagacttcat tgatttgaac tcatccatac acaacaccgt cggaaggaag ttcatacgtg
    22921 gctggtaatg cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc gagacgaagt
    22981 acttcttagc gattagttta cgtgattgat tttcgtacag ttccatgaga atcgcctgct
    23041 tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca gaaaaggtcc
    23101 gtccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtc aacttttctt
    23161 tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaatcgatc
    23221 cacagtgttc acaatgggaa ggtgagtcat ccaatctccc tttaataaac tgcgcttgaa
    23281 cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta
    23341 atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag ggtgacgttt
    23401 tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaaagtg
    23461 ttagtagaaa atcgctttcg attttccacc aacacaaaat attatagaac cataaaaatt
    23521 tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat
    23581 ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc
    23641 aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat
    23701 tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca
    23761 gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac
    23821 ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc
    23881 cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg
    23941 tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa
    24001 aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg
    24061 tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat
    24121 aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga
    24181 tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc
    24241 atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat
    24301 aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc
    24361 atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata
    24421 taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact
    24481 atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc
    24541 tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat
    24601 caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt
    24661 ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg
    24721 caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct
    24781 gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg
    24841 accagaacca aatgttttgg taactttttt catcgttaat atttttttca tttttattaa
    24901 cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct
    24961 agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca
    25021 aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga
    25081 acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt
    25141 ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac
    25201 atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag
    25261 gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac
    25321 tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca
    25381 ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac
    25441 gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg
    25501 acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct
    25561 gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc
    25621 gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg
    25681 ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg
    25741 tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac
    25801 aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca
    25861 ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc
    25921 aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc
    25981 tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg
    26041 acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat
    26101 aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa
    26161 actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga
    26221 taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt
    26281 ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt
    26341 ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa
    26401 aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg
    26461 atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag aactgatcat
    26521 gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta
    26581 taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc
    26641 catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt
    26701 gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga cgattacagt
    26761 tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga
    26821 aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag
    26881 atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta
    26941 tcttttttct tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa
    27001 cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa
    27061 gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca
    27121 tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt
    27181 ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag
    27241 aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca
    27301 gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg
    27361 gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt
    27421 ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt
    27481 attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat
    27541 ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg
    27601 aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag
    27661 cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga
    27721 atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac
    27781 aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata
    27841 acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt
    27901 tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg
    27961 ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag
    28021 atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg
    28081 gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga
    28141 tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc
    28201 ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt
    28261 tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt
    28321 taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc
    28381 ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt
    28441 cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt
    28501 atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa
    28561 tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc
    28621 ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc
    28681 gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat
    28741 tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt
    28801 atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt
    28861 tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc
    28921 taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc
    28981 tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc
    29041 tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc
    29101 aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg
    29161 attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc
    29221 ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc
    29281 aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc
    29341 ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta cttataa
//
LOCUS       DAZKPO010000032        29017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000032 DAZKPO010000000
VERSION     DAZKPO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.032"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..746
                     /locus_tag="ACG8NZ_001335"
     CDS             <1..746
                     /locus_tag="ACG8NZ_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFQ0539020.1"
                     /translation="ELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSG
                     LTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLT
                     PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1099..1947)
                     /locus_tag="ACG8NZ_001336"
                     /pseudo
     CDS             complement(1099..1947)
                     /locus_tag="ACG8NZ_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2100..3212
                     /locus_tag="ACG8NZ_001337"
     CDS             2100..3212
                     /locus_tag="ACG8NZ_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0539021.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3290..3583)
                     /locus_tag="ACG8NZ_001338"
     CDS             complement(3290..3583)
                     /locus_tag="ACG8NZ_001338"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0539022.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3751..4065
                     /locus_tag="ACG8NZ_001339"
     CDS             3751..4065
                     /locus_tag="ACG8NZ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0539023.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4086..6065
                     /locus_tag="ACG8NZ_001340"
     CDS             4086..6065
                     /locus_tag="ACG8NZ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0539024.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6078..6404
                     /locus_tag="ACG8NZ_001341"
     CDS             6078..6404
                     /locus_tag="ACG8NZ_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0539025.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6420..6938
                     /locus_tag="ACG8NZ_001342"
     CDS             6420..6938
                     /locus_tag="ACG8NZ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539026.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6938..8290
                     /gene="celB"
                     /locus_tag="ACG8NZ_001343"
     CDS             6938..8290
                     /gene="celB"
                     /locus_tag="ACG8NZ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0539027.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8374..9879)
                     /locus_tag="ACG8NZ_001344"
     CDS             complement(8374..9879)
                     /locus_tag="ACG8NZ_001344"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0539028.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9971..10561)
                     /locus_tag="ACG8NZ_001345"
     CDS             complement(9971..10561)
                     /locus_tag="ACG8NZ_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0539029.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10740..12386
                     /locus_tag="ACG8NZ_001346"
     CDS             10740..12386
                     /locus_tag="ACG8NZ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0539030.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12435..13154
                     /locus_tag="ACG8NZ_001347"
     CDS             12435..13154
                     /locus_tag="ACG8NZ_001347"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0539031.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13633..14580
                     /locus_tag="ACG8NZ_001348"
     CDS             13633..14580
                     /locus_tag="ACG8NZ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539032.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14592..15512
                     /locus_tag="ACG8NZ_001349"
     CDS             14592..15512
                     /locus_tag="ACG8NZ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539033.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15553..17010
                     /locus_tag="ACG8NZ_001350"
     CDS             15553..17010
                     /locus_tag="ACG8NZ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0539034.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17355..17969
                     /locus_tag="ACG8NZ_001351"
     CDS             17355..17969
                     /locus_tag="ACG8NZ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0539035.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17962..19686
                     /locus_tag="ACG8NZ_001352"
     CDS             17962..19686
                     /locus_tag="ACG8NZ_001352"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0539036.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19683..21134
                     /locus_tag="ACG8NZ_001353"
     CDS             19683..21134
                     /locus_tag="ACG8NZ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0539037.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21607..23760)
                     /locus_tag="ACG8NZ_001354"
     CDS             complement(21607..23760)
                     /locus_tag="ACG8NZ_001354"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0539038.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23762..24808)
                     /locus_tag="ACG8NZ_001355"
     CDS             complement(23762..24808)
                     /locus_tag="ACG8NZ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0539039.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24958..26247
                     /locus_tag="ACG8NZ_001356"
     CDS             24958..26247
                     /locus_tag="ACG8NZ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0539040.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26260..28962
                     /locus_tag="ACG8NZ_001357"
     CDS             26260..28962
                     /locus_tag="ACG8NZ_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0539041.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9435 a         5016 c         5876 g         8690 t
ORIGIN      
        1 tagaattgac gaaagcaaac cgacttacac caggcggtgt cgtgttgaca aagatcgatg
       61 accaaagcgg ggaagtcctg caaggagctg tttttgagtt acaagatgcc aatggaaccg
      121 ttctgcagtc aggtctaacc acaggagcag atggaaaact ggcgattgac ggcttagcac
      181 caggagtgta tcagctggta gaaacccaag cgccaacggg gtatgaattg gatgcgacac
      241 caatcgagtt tgagatcgaa agaagtcaga cagcggtggt agaattgacg aaagaaaatc
      301 gacttacacc aggcggtgtc gtgttgacaa agatcgatga ccaaagcgga gaagtcctgc
      361 aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca ggtctaacca
      421 caggagcaga tggaaaactg gcgatagatg gcttagcacc aggagcatat cagctggtag
      481 aaactcaagc gccaacgggg tatgaattgg atgcgacacc agtaactttc aagatcgaaa
      541 aagaacaaga agtggcgatc tttttaacaa aagagaatag aaaagttgct gatagctcag
      601 atataagagg aaacaatcca acaagcagtg gaaataagca tcttccaaaa actggagaaa
      661 ccatcattag ccagtttata ttgtcaatat tgggtgtgct acttgttttc atcagtataa
      721 aatttagaaa gaaacgaaat atatgagcta aaataaaaaa ttgaataagc atagttttta
      781 aaatcaacta gtacccatca attgaatgga aactaccgat tttagaaggg gtatacaaac
      841 aaatattagt atcggagtag agaaggttga acccaaccag taaataagta aagacaattt
      901 tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa tactagttaa taaaaaatat
      961 acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt tttttgccta atgactcaag
     1021 tttattgggt aatactttga ttttctttct acaaataatc agcgatcagt tgtaattttt
     1081 tctctagttt taatggctct ttgtcaataa ggagaaaaac aagtcgggtc ttttgtctcg
     1141 gaaagcttca accaactcgt gaataaaggt tacgcctcct tgaaaggcgt ggaaaaacca
     1201 agtaatcgat caatcaccaa tttcgcattt ggtagaacat gtttggtgag ctgaaagtag
     1261 gtggcgttca tgtctgtaac taagaattct atttctttag gattagtgca gctaaggaaa
     1321 tagtttgtta atcgaggaat tttttgtgta ggtaaatttt ctcgattaat ttttctgtct
     1381 cgccatccgc ggaaataaaa ctcagtttat cctcaacgga aacgtgagaa cgaaattcac
     1441 aaccctcaac actcaagaca gaattctttt ggaagacttt ggtaaatagc ttctcaactc
     1501 tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa cttttcgaaa taaaaaataa
     1561 cgacactttt tcagtcagta aagaagtgat tttatgtctg acatgatttg aaatcgagtg
     1621 ccttggacgg ataaaataac tttgagcgtt ccaatggctt cgacagtttt tacaggtata
     1681 gcgctgcttt tttaggcgta taaccattgg catatgatta tattattcaa agtggacaat
     1741 ggtttccttc ttcttgtttt tcaccaccat taatttccta tttccatcta ccacagcaga
     1801 accacaattt ctacagacac atggagcagg gaagagaact gcagcaacga tcaatgtttt
     1861 tttcttctgt aaggtttcgt aagagacctc tgtaatcatc aaatttttgt ctattgatct
     1921 cagaattttt ttgatagaac cattcataaa gcatatcgtt ctctcagttg tttattttgt
     1981 ggtgatttaa tcatacaaga aaacgatatg tttttttata aaaacatgaa taggaaaaat
     2041 agttgacaag aaaatgacaa cgccatatga tgttccatag tatttacaac ctgtcataga
     2101 tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca ggtggatttt tatcagtctc
     2161 tacaactaga tccgtttatt ttaaaacaaa aaatacgaga agcagcttct aaaaaagaaa
     2221 agtgcatgta tatttgttca ttgtttttac gatcattatt catcgtattg tttgccattt
     2281 gttttatcat tataattaca acattatttg aatcgaagca taaaccatac gctgtggttt
     2341 tattctgtat gttgatgagt attcgttttg ttgactttgg ttataagatt tcccattcga
     2401 tcataggttt agcaatcgtc atgttctcac tattagtagc accgtatgtc cagctgatca
     2461 aatggtcagt tatgggcatg ttgattcatt ttattttact aagcagcata ttaatggcaa
     2521 ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg cttttcatat ctctttattg
     2581 tttattcgtt acctaaagat tcgttgaaca agaatttctt tacacaaaca agtagcttat
     2641 tatttttgtt tttttgttgg ttttcagttc tcctttacag aaaacatcga gaaaagaaca
     2701 aagacaaaag cttgtttaaa gagatttttt tgaaaggcat gtattcgcag gaaaaaattt
     2761 ggatgctcat ctatgcattt ggcattagtc ttttgatcgt tgcaggagag tatgtccctt
     2821 ttcagcgatt gatgtgggca gggtttgcat tttcttctat tgtatctagc tatggattga
     2881 tgtcgattgg tttcaaagaa cgagcagtag accgaatcat tggctcattg attggatgtg
     2941 ttttatttat tggtatcagc caatttattc cttttaactg ggtcggtata ttaggtggtc
     3001 tagctttagg tgtttgttct acctatagat ataaaacagt ttttaattct tttggtgctt
     3061 tggcaatcac tgccagttta ttcggggttc caggagcagt aacgatccgc atttttgaga
     3121 atatccttgg ggtatgttta ggaatcatgt atattggggt gactgaaata cttatccgaa
     3181 aaataagaga aaaacacggt ttgaatcatt agatgagatc gaataaaaaa taaggaaaaa
     3241 gtgaagatac acatggatga taaattgcta tcattccatg tgtatctttt tacggtaaat
     3301 cagtgaaacg ttcgactttc atttttaatt tatacttttt acggagttcg tttatttgag
     3361 ccatcatttc ggatttgtga tgcttttcga ttgcttcttc atctttccaa cggtcgatca
     3421 gtaaaagaga ttctggatct tctagtggaa agtaatagtc atatttctcg tttccttctt
     3481 ctgcacgaat cgctttgaca atgtttcttt cgaccatttc ttctgcaaat tttctggctt
     3541 gtccatttgt acttgtatac aaaatattca ctgtgatact cactaaatcc cctcctgaaa
     3601 aaattctatc actagtatat cacggtgcca gaaattatta ttcaagatat gatacacatt
     3661 ttttccgcaa tgctacggaa atatatattt tgaatagaaa acgcttacaa gatatgcttg
     3721 gtttataaaa ataaaaggag tgattgattt atgaaaaaag cattgattat ttgtgcggca
     3781 ggaatgtcat cctcgatgat ggcctcaaag acaacggatt tctttaagca aaaaagagaa
     3841 gaggtactcg tagatgcagt ttcagctaca gaaggcgata atatgattaa gaatagtgat
     3901 tttgatttat ttctgatcag cccgcaaacg acgatgtttt tggataaatt caaaaaatta
     3961 ggagaatctg ttggaaagcc tgttgttagt atccctttcc aagcgtatgt cccaatccct
     4021 acaggcatcc agaagttagc agaggtgatc gaagagaaca tatgatggaa taggagtgga
     4081 tgataatgaa acagaaggaa aaattgctgt tgagatattt gattgatcac aagaaggagt
     4141 atgtcaccag tcaacgttta gcttctgaat tgttattatc tgatcggaca atccgtaatt
     4201 acttgcagcg gatcaaagag ttagtagaaa aaaacggagg taagatcatt gctaaaccag
     4261 gttatggcta ccaactgcat attttacaaa gactgacatt tgacttgttt ttgagtcggc
     4321 aagaaatagt acctggatat gctagagaac cgcaaggatt ttatgagtcc gaagatcgac
     4381 aaaagtatat attgaaccaa ttgcttttgg aagatgccat tctttttatg gatgatttgg
     4441 cagaagagct ttatatcagt cgttccagtt tgacaaagga catgcaagaa atcaaggagc
     4501 gattgattcc atattcacta aaaatcgttt ctaagcatgg tcaagggaca tggatcgaag
     4561 gggaagaaag aaatcgacgg cattttatca tggatacttt ttttggaaac cattatggaa
     4621 attcattgaa agaatattta gggcaaagcc gttttttccc agacatcagt tttgaagagc
     4681 tagtcattat cattctcgat gaaacaagag aagctaagtt aaaagtttcg gattttatca
     4741 tacagaatct gactttacac ttggctttgg ggatcaaacg gctccgagaa gggtttgaaa
     4801 tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt agagtatcaa gtggcgcagc
     4861 ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt tcccgaagaa gaaattgctt
     4921 atttgacatt acatctcatg gctaagtcga atcacaaaga aaatcaaaat gatcaggaac
     4981 tatcaaagga attagcgaaa gttctgaaaa gattgtctca ggaatttcat ttttcattag
     5041 tagaggatct ccagcttttc aatggactac tcgatcattt gtcaccgatg atgattcgat
     5101 tgagccgagg tattcgcatt gagaacccat tgactgagga gataaagaaa gaaaatccca
     5161 aagtctttga cgcagtaaaa aggcatttca gcaacatgcc ggctttgaaa aactatacca
     5221 tcaatgaaga tgaatgggcc tatttggctt tgcatctcat ggcggcattg gaaaaagaaa
     5281 gagctgcgca taaactacat gcattgatta tctgtgcaac gggttacggc agtgcccaac
     5341 tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat tacagtggta agtgtaaaag
     5401 gctattatga gatcaatgaa acaactttaa aaggaatcga cctgattatt tcttctattg
     5461 atctttcgac aatgtttttc aaaattccag ttttacatgt cagtattttt ttgaatgaag
     5521 atgatgttcg taaaatacga aaggtagttg gagaatctat accctatcat tcctcagata
     5581 gaccgatacc agcattttct ctacagcaaa aaaagcagat ttacaccgaa cagttgtctg
     5641 aaaaattttt caagacttat acgtacgaac cgaaaaagga agaaattctt catgatttgc
     5701 tgcaaaagct gagcataaat gaagaagaag gatatataag agaaatgcga aagcagatcc
     5761 agcaacgtga aaatatggga caaatcatat tcagtgatac agtggcagtc ccacatcccg
     5821 ctgttccagt tggaatcgaa acaaaggtag ccgttgcatt gattccttct gggatgcagt
     5881 gggatcaata tcaagaaatt cattttgttt ttttagtttc gccttcttat gtcgaaaatg
     5941 aaggaattac ggtcgtgact aaagcaatcg ttaggttagt tgaccagcta aaagtacagc
     6001 aggaaatctt agctgaacct atttttgaga atttcagcag taaatttatc aaactgatcg
     6061 aataaggagg aaaacagatg gctgaaaaaa taaacagtga aacactacaa gtgacttctt
     6121 ttgatatcat tttccatagc ggaaatgcac ggacactgat acatgaagca tttcgctcga
     6181 tgcgtaaaga gatgtttgaa gaagtagaag agaagttgcg acaagcaaat gaagagattc
     6241 ttcatgcgca tcagtcacaa actcaacttc tgaaagaata tgccagcggg acaaaaatag
     6301 aaatggaaat tatcatggtc catgcacaag atcatctgat gacgacgatg acattattgg
     6361 aagttgcaca ggaaatgaat tatttgtatc aaaaaaataa atagaaagga aggatcattg
     6421 tgcaggatac agtaaaaagg aaaatcgaat tagaaaaaaa gcaacttagt ttgaaaaaca
     6481 tgtatttcaa tcgttatcta atcgtacgtt acctgactgc ctttttcttt ttcatgaaca
     6541 tgcaatggat gattctgctt ctatcagcta aaagtttagg aagtagttta ccgatgattc
     6601 tgattttagc tattttgcct gcagtaggag aacaagtaaa gctttatcga aaacaccaaa
     6661 cgaacgttcc atggacaaag tggtattttt tgttccagag tgtctttaat ttattagtga
     6721 ttccagtgtt gtttacctca ggatttacat tcttgtatcc ttttatggca aataacaatg
     6781 gaggacagtt atttgttttc attcttgttg taagcggtat ttttgccagt gcatttatac
     6841 agtatcgttt aaagaaaatt tcactcaacc aagaccaaca gtatatccgt attaaacaat
     6901 atgaaaaagc gttatattta ggaaaggaga ataactaatg tttgattttt tgcagaaata
     6961 cctgatggga cctatgggga aaattgcgca acttaaaatc gttcgggctg tcatggcagc
     7021 ggggatggct tctattcctt tcacgattgt aggctcgatg tttttggttt taaatatttt
     7081 gccattaccg ttcccatttt tagaaggatt ttttaatgca acttttttca aagttagtga
     7141 cttgtacatg atcgtcaata caatgacgat ggggatttta tctatttact ttgccattgt
     7201 gtttgcttat gaattaacgt caattgaacg agatgaacaa aaattaaatg ttaatccgct
     7261 cacgggggca ttactatctg tttttgcatt ctttatgtgt atccctgaat tgatcatgac
     7321 tgatggtaaa atcagcttga ttgcttctat caatgagaca gaaactgtag taaatggtat
     7381 taggatgggc tcttttgtcg aacgtttagg aacttcagga atctttacag ctattattat
     7441 gtcgattatt gcggtcgaat tgtattgtat gtgtgtcaag agaaattggg tcataaaaat
     7501 gccagatgtt gttccgccag gagtttctcg ttctttcact gctctgatcc caacatttgt
     7561 cattgcgttt gtggtgatga tcatcaatgg attattagtt gcactaggaa cggatatctt
     7621 caaaatgatt gctatcccat ttgggtttgt tacgcagttg acgaatacat ggcttggtgt
     7681 catggtgatt tatttcttga tccatgcatt gtggatcgta gggatccatg gagcgaatat
     7741 catcacttct ttcttgaccc cgatcgtatt agctaatatg gcagctaacg cacaaggcgc
     7801 aaattatccg ttggcaggag aattcaacaa ttcttatgta accgttggtg gatcaggtgc
     7861 gactttagga ttgatcatct ttattgcatt cctcgcgaaa tctgatcagc tgaaagtgtt
     7921 aggaaaagcc tcacttgtac caggattatt caatatcaat gaaccaatca ttttcgggat
     7981 gccaatcgtc tacaatccct atttagcggt tccgtttttc ttagctccga tggcttctgc
     8041 tacactcgct tattttgcca ttcggctgga aatcgtcaaa ccgatgcttg cacagatgcc
     8101 gtggccttca ccagtcggga tcggagcatt tgttggtagt ggtggtgatt ggaaagctgc
     8161 tgtgttagca gtcatttgtg caatcatcgc attcattatc tggttcccat ttatcaaatt
     8221 ctatgataat aaattattaa atgaagaaag agaaaaggca gcagaattag ctagtgaaaa
     8281 gttaaaatga tttctaagaa aaaaggaact gtgacaaaaa atcacagtcc ctttttttct
     8341 tagaaatggg aaaccactag tatataaata ccattattct tcaatcaacc gcttcacgat
     8401 tgcataatcc ccttcataag gttcatctac accattgatt ttttgatagg gatccacgcc
     8461 ttttcccgca ataacgactg aatcttgcgg agcgctttct gctaaagcgc gtcgaatcgc
     8521 ttcaggacga tccatttcat aatgaacgat caaatctgga ttagtaattg cttcattgat
     8581 ttcttccgcg atttttttcg gatcttcaaa tgctgggtca tctgccgtca aaaaggcgat
     8641 gtcagctgtt tctgatagca cttttccaaa atcatgtcga cgggagattg ctttgtttcc
     8701 tgggctgcct aatacaacaa ttacacggcc atctggatgt tcatttttgg caaatttcag
     8761 caatgttttc aagcttagat aattatgtgc atagtccaca tagacatgcg ctccattgga
     8821 ttggatcaat tgatccattc ggcctggtac acgagcttct tttaatccgt cttgtgcatc
     8881 ttgactggag gcacctgtta gtgctgcggc aatgatcgca ctggctgcat tttcttggtt
     8941 gaatcctcca gccagcaaaa tatcataagc agtatcgcag atagctaagc gatcttcatg
     9001 agaagataaa gtaaaaccgt gagtaccttt atctgaacgg atcacttgat aattgctttc
     9061 ttccgaacga ccataagtga tcgttggaat ctggaataga tcggctgttt ccctaagtaa
     9121 atcaaaatag tcactttcat gatttaaaac aactgttttt gaattcaaga tcaattgtcg
     9181 cttgcaatag aaatagtcat caaatgtcgg atgttcgatc ggactgatat ggtcaggcga
     9241 gatattcaag aagattccga catctaaagt caagccgtat acgcgttgtg ttttgtaagc
     9301 ttgtgaagaa acttccatca ctagatgagt cataccattt tcaactgctt ctgacatcat
     9361 acgatataaa tctaatgact caggcgttgt caaatgggat ttgaaatatg ttttgccatc
     9421 taacgttgta ttcatagtag aaagcaatgc tgttttttta tttgttgtgt gatccaagat
     9481 tgcttttgta aagtacgcag cagttgtttt accttttgtt ccagtgaatc cgatcacttt
     9541 caatttattt tgcggatagt cgtaaaaagc catagcaaga agcgccattg ctttacggat
     9601 atccgttacg atgattccta ctgctgaatt ttcgtatggt tgttctgata cataatattg
     9661 aataccctgt tcgattgctg aatccaaata ttcttctttg aaattcattc ctttacagaa
     9721 aaacaaagta gaagcatcag cttttcttga atcataactt aactgaagaa attcagcttt
     9781 ttctggtaca tcaagatgcc agcctcttgt tgaaacaaat tcttttaata gattttcttt
     9841 taatagtaaa tgacggatgt cttctaaaga gaagctcatt gattgatccc tcgctttatt
     9901 caatttcttg gtttattata gcacactctt tttaatatat cgtttgtttc cgctatgaca
     9961 aaatccagct ttattcaaat tttcttgaca caggggacaa gcggatgagt attttcataa
    10021 acaatgaact gactgtaata gataggagaa cgaatagtgt ctccaacaat ccaaaaacat
    10081 aataattcaa aaagacgata agactattgg taagtaaaag tccttgcggc attggaagcc
    10141 gaaaaaattt gtggaaaata atcggcggta tcgttatacc gccatttgag gccccgactg
    10201 tgtacaatgt gtaaacccca agactgaaca aaagacttcc tgaaatcagt gaaaagaaat
    10261 gtgaggagat catttccact tttggaacaa tctctaaaac aacaggaaaa agcagacttc
    10321 ccgctacagt cttaaaaaat actgattttc ctaataggac agcggctaga agcagcatca
    10381 taagattgat gctccaaaca gtaactgagc gttggataga aaaggccgtt tcaactaaat
    10441 gtccgatggc gcccactccg gcggaagcta tatcgtgagg gagcaagaac caattcaatc
    10501 caactgtgat aaagaaaaga cctaaaaata taacgggtag tttttttatt tcttgtgtca
    10561 ttgatatctc ctataaatgt tagccataaa aggctgtata tttctttact ttaccaaaaa
    10621 aacgaaaccg aattcaaaaa aacaattgtt cttataaaag ttatataaaa tcgattgcca
    10681 gtttctgttt taccaattaa aatgatagta atgttttgta gaaagttgga atctgaccga
    10741 tggataatca aagaaaacca gcattagatc aattaacacc gcaagaaaaa atggcacgag
    10801 gctctgcttg gatgaccgcc agtaatatca tttcaagatt gctaggcgct gtctatatca
    10861 ttccctggta tgcttggatg ggcgaaaatg ctaaggctgc caatggatta ttcaatatgg
    10921 gatacaatat atatgcattg tttctactga tctctacagc aggaatacct gctgcgattg
    10981 caaaacaaac tgcacgctat aattcattga atgaatatgg cacttcacga cgattattta
    11041 tccgtgcatt acagatgatg ggcgggttag gcctgttgtt tgctttgttc atgtacatcg
    11101 cctctccttg gctagctcat gcttctggtg gcggagaaga actgattcca acgatgcgtt
    11161 ctttgagtat cgctgtattg gtttttcctt gtatgagtgt gatacgaggg tatttgcaag
    11221 gcaatcaaga aatgatgcct tatgcgctgt cgcagattgt agaacaggtg gcacgtattt
    11281 tttatatgct attggctaca ttcatcatca tgaaagtcat ggatggtgac tatgtgacgg
    11341 cggtcacgca atcaactttc gcggcattta tcggtatgct agcaagtatt cttgtccttt
    11401 tgtatttttt gaagaaacat caagcgtata catcggcgtt tattcactac agcgaaaaca
    11461 aaataaatat tgcaacgaag gaattgttgt tagataccgt taaagaagcg attccgttta
    11521 ttattgtggg ttcaggggta acgatcttca aattagtcga tcagttcact tttatgaaga
    11581 tcatgtctgc atccaccgag tattccaatg cacagttact ggatcttttt tcgattttta
    11641 gtgccaatcc agataagttg acgatggtag ttattgcatt ggcaacatcg atcgcgtcta
    11701 ctgggttacc gttgattaca gaagctgtta cgttgaaaga tcgagtaggt ctggcaaaat
    11761 tgacaagcgg aaatttacag ttgttttcat tttttatgtt tccggcgaca tttggtgtta
    11821 tgttacttgc ctatccgcta aatacattat tttatacacc ggattctcta ggaagtaatg
    11881 tactgatcca agcaagtttt gtaggactct tcctaggact atatatgcta gtttccaaca
    11941 tgctgcaagg aatgtttgaa aacaaggcgg caattaatta tttggttatt gggtttctag
    12001 tgaaactcct tcttcaatac ccagccattc gtttatttga agtatacggc cctttattgg
    12061 cgacaatgat cggatttgct gtgtcttgta ccttgatctt aaagagaatc catgaagtag
    12121 cccgctttaa accaagtttt gtctggcgca ggacattgct gatctttatt cttaccttga
    12181 ttatgctttt ggcttcgtgg atcacaaaga tgatttttgg tgctttttta aacgaagata
    12241 gcaaattcca gtctctattg ttgattcttg tagtagcagg tataggggct ttagtgtatg
    12301 gttatttagc tttgaaaatc cgcttggcag aaagactact aggaccagga atagcgcgtt
    12361 tacgacataa attaaaaata aaataagtga atcagaagag ctagattgat gctcttttga
    12421 aatgggggaa acgcatgcgt ttagataagt ttttagcaga tatgggactt ggcagccgca
    12481 aagaagtcaa aggattaatc aaaaaaggaa cgatcgaagt caatggacaa gtggtgaaaa
    12541 gcgataaagc gcaagtaaat gaattcgaag atgcggttac ttatttagga gaaatcatta
    12601 cgtatcaaaa agatttttac tacattttgc ataaacctgc aggagtcatt tctgctactc
    12661 aggataatca tgatcaaacc gtaatcgatt tattggaaga ccaggattat cgggaagatc
    12721 tttttccagt cggccgatta gataaagaca cagagggcct cttgatttta acgaatgacg
    12781 gggtgtttgc ccatcagtta ctatcaccta aaaaacatgt ggagaaagaa tatctagctg
    12841 aggttagcgg tataatgaca gaagaagata tccgtttatt tgcagaagga ctgattattg
    12901 atggcgatga gcagacattg ccggcaaaac tccaaattga tcaagtagat caagagacag
    12961 aaacttcaat tatccgtctg attttgcatg aaggaaagtt tcaccaagtt aagcgaatgg
    13021 tcaaagcagt aggaaaagaa gtcttatatt taaaaagaat ccgtatggga aaattttggc
    13081 tgcctaaaga tttgaataaa gggcaatatc gacccatgac caaagaagag ctagaacaag
    13141 tgagagaaaa ataattgaat ggatagctaa aagaataagc agagtagttg tagcgccatc
    13201 tgaaaagcag acttgtccgt ctgactttcg gatggctttt ttgtgaaatt tcggcttatt
    13261 tccgtagaag ctacttctgg aacaccgttt gttcggaata agggactgtg acaagacttt
    13321 tgttacagtc cccattgttc cttgtgactc agagttagat agtaaggaca cgatctccct
    13381 aatactcagg ccaaaacgct gaattcacaa cctctgataa acggtgttca atcagctgac
    13441 cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttcccgt ttttttctct
    13501 aatactcagg ccaaaacgct gatttcacaa cctctcctat actttgtttc attttcccta
    13561 tactttgttt atttattctc attattgaaa gcgctataat tacgttgcga atgaatcgaa
    13621 aggggttttt agatgaaaaa acgaaagggg ttcaatcatt tttttatgaa tctatggcgt
    13681 tataaagcac ttgtcctgat ggctgtaccg ggtatgcttt ggatgatttt tttcttttac
    13741 atacctgtaa tggcgaatgt cgtagcattc aaaaattttc atatttcagc agatgggttc
    13801 ttggctagct tgagggaaag tccttgggtg ggttgggaaa atttcaaatt tttattttct
    13861 tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc atttatcctt
    13921 ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag aaataaacga
    13981 ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg ggtcattatc
    14041 agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca gtggatcact
    14101 ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc gtttatcttg
    14161 gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt tgcatctgtt
    14221 atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag caaatggcag
    14281 cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt aacgatttta
    14341 gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc taggaattcc
    14401 ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg cctgacatcc
    14461 acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt cggctttgtc
    14521 ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc attattctaa
    14581 ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct ttcaataaga
    14641 cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt attttgccat
    14701 ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact cacgggtaca
    14761 gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac cagatgcagg
    14821 ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt acactgatca
    14881 atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt ccattccgtc
    14941 gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg gtggccaatt
    15001 atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg atcttgccaa
    15061 tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt tcggtctctg
    15121 attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata tttgttcaga
    15181 tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc gcattgggtt
    15241 actggaatga ctggttcaat gctttactat acatccaaga tgataagcta gttccactac
    15301 agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat tcaggagcag
    15361 gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga atggcgatcg
    15421 tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag cattttgtca
    15481 aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat cataaaatta
    15541 ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc agcgttgttg
    15601 ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga agctagcaat
    15661 tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga tcaattgatg
    15721 gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat caattacatt
    15781 ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga aaattatgat
    15841 atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc tgatttgact
    15901 gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc atatattaaa
    15961 ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt ctttgctcaa
    16021 caagtactta cttttaacaa agctttacta gataaatacg atctttcaat tgatggaatc
    16081 caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga accaaacacg
    16141 gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta tcctttaggg
    16201 aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat caaccaatac
    16261 gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa acaaggactc
    16321 attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa tacttggcta
    16381 atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac aaatgcagca
    16441 aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc tcaagcacaa
    16501 atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt agaacttcta
    16561 gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat cgagggagaa
    16621 gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg ttatcagcca
    16681 aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac acaagagtct
    16741 attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga aacttcaccg
    16801 attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa tatttctaat
    16861 gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga tgaaacatta
    16921 ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa agaaatgcaa
    16981 aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg gttgtgacaa
    17041 aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt ttctcggtat
    17101 taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc ttactactcg
    17161 aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct aaccctcggt
    17221 attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc tctaataatc
    17281 aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata aacttaatgt
    17341 ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg atctgggtca
    17401 ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc atctttggat
    17461 taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc gagtataaag
    17521 aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga ggcaatctgc
    17581 tatttagttt atttttatct ggtactgctt tattgagtta caatttatat ttatctgttc
    17641 aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc ttgctctgcc
    17701 tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc tctgttccta
    17761 atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt ctaaaaataa
    17821 ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg atcctgtttg
    17881 gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg cgtgaagaat
    17941 tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa aggaacctta
    18001 ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat gactgctacc
    18061 gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa catgacaagt
    18121 gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag taggatcatc
    18181 aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta cttaacaaaa
    18241 cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga tgaattagtt
    18301 tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag tgcgatatat
    18361 atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa aggtggggaa
    18421 ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc tatcacacaa
    18481 ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact agatatgatt
    18541 ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg aacgacaaat
    18601 cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag acaagaaatg
    18661 atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct ggaaaaaaac
    18721 aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt actagataaa
    18781 aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg tatcattatt
    18841 ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc acagcaagta
    18901 tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac gcggatcgac
    18961 ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga aatgttggat
    19021 agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca agacgcacat
    19081 atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt agaatatatc
    19141 cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta tgcgttttcc
    19201 gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga agaactggac
    19261 ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt agcctatcat
    19321 tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat ccagccatta
    19381 gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc gctaagtgtc
    19441 aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg aaaagggatg
    19501 actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt ggaacttcac
    19561 ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg cagcagttat
    19621 tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac attcaaggag
    19681 ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg aatgggttaa
    19741 aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca gagaatgcca
    19801 aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg gatgtgacaa
    19861 tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt cataattttg
    19921 aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt atgcaattag
    19981 gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa agcctcaaga
    20041 aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa atctatcaag
    20101 aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa gaagaaataa
    20161 taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca agaactcatg
    20221 atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat caacgaaatt
    20281 acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat ggcttctgtt
    20341 gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg gaagagacag
    20401 attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta cagttgcatc
    20461 aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa acaaaagatc
    20521 aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaatta gaagtcgctc
    20581 aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg ccggaagata
    20641 tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt gatctggaag
    20701 atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg caagaacttc
    20761 agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac agtgacaatg
    20821 tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca ctcaaagaag
    20881 tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa aaagaaacga
    20941 aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa caactgcttt
    21001 tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac aacaccacgt
    21061 atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac cgagaaaaca
    21121 gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa attgtctaga
    21181 aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat gacctctcga
    21241 taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtggaa
    21301 agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc acgacctctc
    21361 cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga
    21421 ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt tcacaacctc
    21481 tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa acgaaaaata
    21541 tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct ttttcacaac
    21601 ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat atttcttagg
    21661 gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat cttgatgggt
    21721 aaaatataat ttcgtatatt ctttgtccat ccgtttgatt ttatggataa attgttgctg
    21781 cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa gcaaaacagc
    21841 cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga caggataaaa
    21901 tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat ctccagggta
    21961 acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg gctgtgccat
    22021 ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct ggtttgaaat
    22081 agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct caaaaccata
    22141 tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt ctttatatct
    22201 ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag caaagctgct
    22261 ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa atggttctct
    22321 gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa agatattttg
    22381 atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta gtaccttggc
    22441 aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga ctgattcgtg
    22501 gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc gtccataatt
    22561 tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt caggcatcaa
    22621 gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca atgtcccagg
    22681 cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt ctctatagct
    22741 gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag aataaagagg
    22801 ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac caggttttat
    22861 agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt cataaaatgt
    22921 ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt gttgttgatc
    22981 atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac tgtgagcaag
    23041 gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt gttccgagct
    23101 gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat acaacgggat
    23161 cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat attcaaatct
    23221 caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg tgatttttcc
    23281 ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg ataaaagtaa
    23341 accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc taggtatcaa
    23401 tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc aaaatatact
    23461 ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta gttttccatt
    23521 gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt gatgggtaag
    23581 acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt tttgatccgt
    23641 agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct ggcagttccc
    23701 attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac cgatattcat
    23761 tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta ctatcggatg
    23821 gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta cctaactgtt
    23881 caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct tggatatcat
    23941 caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc aagagattca
    24001 tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca tacttagtga
    24061 tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg tggaaagtca
    24121 aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg gattgtggat
    24181 gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc aaataagagg
    24241 cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt aactcataga
    24301 tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa agtaagtcaa
    24361 gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg attagctctt
    24421 ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc tgtttatctt
    24481 gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg acatatggaa
    24541 gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc ggagaagcgg
    24601 atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc tctaattcag
    24661 tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt tctttttcag
    24721 ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc aaatcattaa
    24781 atattgtctg gtacaatggt tgattcatat aaacacctct taatgatata tcatattcaa
    24841 ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc gtagacataa
    24901 ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt tttattagtg
    24961 gcttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac acaaaaatgt
    25021 ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa tactctgttg
    25081 acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga cattccagcg
    25141 atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc aaaagaagat
    25201 gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta tatcaatatc
    25261 gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca aacagatcat
    25321 acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt atgctaccca
    25381 atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt caatcaatct
    25441 tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga tcatgaacac
    25501 tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat caatgacggg
    25561 cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc tagcgatgat
    25621 acttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt gttaggttat
    25681 gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca aacacagaaa
    25741 ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa tcaaaaaggg
    25801 caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat ggatgacagt
    25861 aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca agaggaagat
    25921 tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta tgaaggaaag
    25981 tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc gattgcatta
    26041 gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt gaatttactt
    26101 gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa caatcctgaa
    26161 aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt agtcatggat
    26221 tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa agtatatatc
    26281 atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca tcacatgcga
    26341 ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc cttcaacagt
    26401 tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag gccagaaaaa
    26461 agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc attttatatc
    26521 ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct gattggcatg
    26581 gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga tacatttgga
    26641 aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc agcgtttggc
    26701 cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa ctattcttcc
    26761 caatactcag agatgtggtg gaaaggacct gatcaaacag caatctttgg tctgctattt
    26821 gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc tcttgccttt
    26881 tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt attgatgatg
    26941 aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca tctcgcaaat
    27001 gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct ggaagcagtt
    27061 caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag tcaagaaacg
    27121 gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa acaatggaat
    27181 accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat ggcttatgaa
    27241 gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt aatgcaaaat
    27301 catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga aatgatacct
    27361 cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt agaacagctg
    27421 accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt cattgtaaat
    27481 actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga aagaaaaaga
    27541 ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc taaacgtaag
    27601 tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga agaaactgtc
    27661 caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc aagaatgatc
    27721 aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt cgcgcttgta
    27781 gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg acgaatcatc
    27841 gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat ggaagatcgc
    27901 aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga tataggaaat
    27961 gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa caaagaaaac
    28021 tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat tctacaggaa
    28081 atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat ggttgtggaa
    28141 tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat aaaaacaatc
    28201 atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga caatcagatg
    28261 aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca tcacgaagca
    28321 gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg ggaaaacccc
    28381 acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta cggtgtaaca
    28441 gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat cgcagtgacg
    28501 ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc agaagcacag
    28561 tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga agaaaaattc
    28621 tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca aatcaaacat
    28681 catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga aacgtttgca
    28741 attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg tttcaatatg
    28801 tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt catattgaat
    28861 ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta tgagattcgg
    28921 acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc ggaacaaaat
    28981 ttgtgtgtgc ggtttcagat ggtggagaaa ttttaga
//
LOCUS       DAZKPO010000033        28866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000033 DAZKPO010000000
VERSION     DAZKPO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.033"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8NZ_001358"
     CDS             85..624
                     /locus_tag="ACG8NZ_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539042.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="ACG8NZ_001359"
     CDS             1644..2813
                     /locus_tag="ACG8NZ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0539043.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="ACG8NZ_001360"
     CDS             3286..3840
                     /locus_tag="ACG8NZ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0539044.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4450)
                     /locus_tag="ACG8NZ_001361"
     CDS             complement(4013..4450)
                     /locus_tag="ACG8NZ_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0539045.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5445
                     /locus_tag="ACG8NZ_001362"
                     /pseudo
     CDS             4498..5445
                     /locus_tag="ACG8NZ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5716..6151
                     /locus_tag="ACG8NZ_001363"
                     /pseudo
     CDS             5716..6151
                     /locus_tag="ACG8NZ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            6167..7000
                     /locus_tag="ACG8NZ_001364"
     CDS             6167..7000
                     /locus_tag="ACG8NZ_001364"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539046.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7245..8270
                     /locus_tag="ACG8NZ_001365"
     CDS             7245..8270
                     /locus_tag="ACG8NZ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539047.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8408..8533
                     /locus_tag="ACG8NZ_001366"
     CDS             8408..8533
                     /locus_tag="ACG8NZ_001366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539048.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8558..9673
                     /locus_tag="ACG8NZ_001367"
                     /pseudo
     CDS             8558..9673
                     /locus_tag="ACG8NZ_001367"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            9756..10652
                     /locus_tag="ACG8NZ_001368"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="ACG8NZ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10668..11492
                     /locus_tag="ACG8NZ_001369"
     CDS             10668..11492
                     /locus_tag="ACG8NZ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539049.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="ACG8NZ_001370"
     CDS             11524..12834
                     /locus_tag="ACG8NZ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0539050.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="ACG8NZ_001371"
     CDS             12982..14241
                     /locus_tag="ACG8NZ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0539051.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="ACG8NZ_001372"
     CDS             14251..15123
                     /locus_tag="ACG8NZ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539052.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="ACG8NZ_001373"
     CDS             15135..15965
                     /locus_tag="ACG8NZ_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539053.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="ACG8NZ_001374"
     CDS             16005..18188
                     /locus_tag="ACG8NZ_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0539054.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="ACG8NZ_001375"
     CDS             18249..19469
                     /locus_tag="ACG8NZ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0539055.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8NZ_001376"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="ACG8NZ_001376"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0539056.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="ACG8NZ_001377"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="ACG8NZ_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="ACG8NZ_001378"
     CDS             22431..22640
                     /locus_tag="ACG8NZ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0539057.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="ACG8NZ_001379"
     CDS             22633..22782
                     /locus_tag="ACG8NZ_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0539058.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="ACG8NZ_001380"
     CDS             22936..23601
                     /locus_tag="ACG8NZ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0539059.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8NZ_001381"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACG8NZ_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0539060.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="ACG8NZ_001382"
     CDS             25304..25537
                     /locus_tag="ACG8NZ_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0539061.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="ACG8NZ_001383"
     CDS             25534..25941
                     /locus_tag="ACG8NZ_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0539062.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="ACG8NZ_001384"
     CDS             complement(27078..27596)
                     /locus_tag="ACG8NZ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0539063.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="ACG8NZ_001385"
     CDS             27963..28211
                     /locus_tag="ACG8NZ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539064.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            28714..>28866
                     /locus_tag="ACG8NZ_001386"
     CDS             28714..>28866
                     /locus_tag="ACG8NZ_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0539065.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         9935 a         4350 c         5290 g         9291 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagcttg gataggctac aattaactag acagaaaaat taaggtgtgt
    28681 agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt
    28741 tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa
    28801 atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa gcaatctaaa
    28861 acgagt
//
LOCUS       DAZKPO010000034        27483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000034 DAZKPO010000000
VERSION     DAZKPO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.034"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..333)
                     /locus_tag="ACG8NZ_001387"
     CDS             complement(<1..333)
                     /locus_tag="ACG8NZ_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539066.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSN"
     gene            complement(405..1685)
                     /locus_tag="ACG8NZ_001388"
     CDS             complement(405..1685)
                     /locus_tag="ACG8NZ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0539067.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(1776..2228)
                     /locus_tag="ACG8NZ_001389"
     CDS             complement(1776..2228)
                     /locus_tag="ACG8NZ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0539068.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2241..2651)
                     /locus_tag="ACG8NZ_001390"
     CDS             complement(2241..2651)
                     /locus_tag="ACG8NZ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0539069.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2926..3138
                     /locus_tag="ACG8NZ_001391"
     CDS             2926..3138
                     /locus_tag="ACG8NZ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0539070.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3141..3353
                     /locus_tag="ACG8NZ_001392"
     CDS             3141..3353
                     /locus_tag="ACG8NZ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539071.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3493..3648
                     /locus_tag="ACG8NZ_001393"
     CDS             3493..3648
                     /locus_tag="ACG8NZ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539072.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3645..3869)
                     /locus_tag="ACG8NZ_001394"
     CDS             complement(3645..3869)
                     /locus_tag="ACG8NZ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0539073.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3947..4135
                     /locus_tag="ACG8NZ_001395"
     CDS             3947..4135
                     /locus_tag="ACG8NZ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539074.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4132..4305
                     /locus_tag="ACG8NZ_001396"
     CDS             4132..4305
                     /locus_tag="ACG8NZ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539075.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            4274..4402
                     /locus_tag="ACG8NZ_001397"
     CDS             4274..4402
                     /locus_tag="ACG8NZ_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539076.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            4461..4796
                     /locus_tag="ACG8NZ_001398"
     CDS             4461..4796
                     /locus_tag="ACG8NZ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539077.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5029..5970
                     /locus_tag="ACG8NZ_001399"
     CDS             5029..5970
                     /locus_tag="ACG8NZ_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0539078.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5972..6862
                     /locus_tag="ACG8NZ_001400"
     CDS             5972..6862
                     /locus_tag="ACG8NZ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0539079.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6905..7705
                     /locus_tag="ACG8NZ_001401"
     CDS             6905..7705
                     /locus_tag="ACG8NZ_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0539080.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7720..8571
                     /locus_tag="ACG8NZ_001402"
     CDS             7720..8571
                     /locus_tag="ACG8NZ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0539081.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8772..9233
                     /locus_tag="ACG8NZ_001403"
     CDS             8772..9233
                     /locus_tag="ACG8NZ_001403"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0539082.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9259..9711
                     /locus_tag="ACG8NZ_001404"
     CDS             9259..9711
                     /locus_tag="ACG8NZ_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539083.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9817..10011
                     /locus_tag="ACG8NZ_001405"
     CDS             9817..10011
                     /locus_tag="ACG8NZ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539084.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            10042..10335
                     /locus_tag="ACG8NZ_001406"
     CDS             10042..10335
                     /locus_tag="ACG8NZ_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539085.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            10266..10805
                     /locus_tag="ACG8NZ_001407"
     CDS             10266..10805
                     /locus_tag="ACG8NZ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0539086.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            10880..11347
                     /locus_tag="ACG8NZ_001408"
     CDS             10880..11347
                     /locus_tag="ACG8NZ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0539087.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            12094..12324
                     /locus_tag="ACG8NZ_001409"
     CDS             12094..12324
                     /locus_tag="ACG8NZ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539088.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            12483..13316
                     /gene="terS"
                     /locus_tag="ACG8NZ_001410"
     CDS             12483..13316
                     /gene="terS"
                     /locus_tag="ACG8NZ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0539089.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13309..14724
                     /locus_tag="ACG8NZ_001411"
     CDS             13309..14724
                     /locus_tag="ACG8NZ_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0539090.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14736..16265
                     /locus_tag="ACG8NZ_001412"
     CDS             14736..16265
                     /locus_tag="ACG8NZ_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0539091.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16183..17235
                     /locus_tag="ACG8NZ_001413"
     CDS             16183..17235
                     /locus_tag="ACG8NZ_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0539092.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17237..17554
                     /locus_tag="ACG8NZ_001414"
     CDS             17237..17554
                     /locus_tag="ACG8NZ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539093.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17681..18304
                     /locus_tag="ACG8NZ_001415"
     CDS             17681..18304
                     /locus_tag="ACG8NZ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0539094.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18319..19209
                     /locus_tag="ACG8NZ_001416"
     CDS             18319..19209
                     /locus_tag="ACG8NZ_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0539095.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19232..19447
                     /locus_tag="ACG8NZ_001417"
     CDS             19232..19447
                     /locus_tag="ACG8NZ_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539096.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19459..19800
                     /locus_tag="ACG8NZ_001418"
     CDS             19459..19800
                     /locus_tag="ACG8NZ_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539097.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19800..20171
                     /locus_tag="ACG8NZ_001419"
     CDS             19800..20171
                     /locus_tag="ACG8NZ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539098.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20164..20559
                     /locus_tag="ACG8NZ_001420"
     CDS             20164..20559
                     /locus_tag="ACG8NZ_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0539099.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20561..20938
                     /locus_tag="ACG8NZ_001421"
     CDS             20561..20938
                     /locus_tag="ACG8NZ_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0539100.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20939..21547
                     /locus_tag="ACG8NZ_001422"
     CDS             20939..21547
                     /locus_tag="ACG8NZ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0539101.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21547..21888
                     /locus_tag="ACG8NZ_001423"
     CDS             21547..21888
                     /locus_tag="ACG8NZ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539102.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22130..24919
                     /locus_tag="ACG8NZ_001424"
     CDS             22130..24919
                     /locus_tag="ACG8NZ_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0539103.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            24909..25649
                     /locus_tag="ACG8NZ_001425"
     CDS             24909..25649
                     /locus_tag="ACG8NZ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0539104.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            25646..>27483
                     /locus_tag="ACG8NZ_001426"
     CDS             25646..>27483
                     /locus_tag="ACG8NZ_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0539105.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9808 a         3956 c         5443 g         8276 t
ORIGIN      
        1 attactatta acgtttttta caagtacatc atcttttata tattttgaaa ttaatgatag
       61 ataaacctga gttgttcgat aggaactttc ttttacagtt ggcttgtatt tttcatccca
      121 tttagttttt aattctccaa aagttagtgg taattcttca tttcgtttta ttttttctgc
      181 gattttttca ttcaaaagta agttagcttg attccaagct tggcgtgaat tgctagataa
      241 agtaacggac acttttctct ttttttcagt taagggatcc gtataacgtt cggtgtactt
      301 aaattttttg tcatttattt tttctaacca cattatttct attcctttcg aatatacgtt
      361 cctattaatt gttaaaagaa aaagcccgaa ggctttttat atatttaatc ttttgacaat
      421 tctaaagatc gtctatattg ttcagcttta gctaattttg taaatgttac tgttccatta
      481 taattttcta aaacaacttt ctctatttcg tctagtgaaa ctttgaaaaa ttcttttctt
      541 tgattaattt tattaacttc ttgttttcta aaatgattat gcaatttagt ttccaaagac
      601 ggagcgtcct ctgaaaaaat catcgcatga acgtcaaatt caaatggaac cgatgcacta
      661 ctcaattctt ttattctatc cataggttct aatcttctag tcataccaat tttatatatg
      721 tcttcaccaa aagacccaat attactaatt acatacacat aaccggctct agtatttaat
      781 tctctttgta atacattttc acgtataact tctaattctt ttaatttttc ttccagttct
      841 tgaattttat ttgcatataa ttctttttcc acatctgcat tagctttttg catatatgcg
      901 attaatttgg aaatttcaga attgaattga cgttgttctt tatcaatacg tttcttttca
      961 cgatcaattt ctctacgaac tttttcctct tctatcatct gttcttttat tgctttttgt
     1021 tgttcttttt cttcggtaat tttcactaaa taagagtaat gaagttctag ttcttgaagt
     1081 ttaaactcta aaagaccctt ccttaattca gcattgtcat tcttgaataa attattaatt
     1141 ttatcaaata tttttatgat tttttcccta gtcgagtcta tattagaaat agttagttta
     1201 ttgagtaatc ctttcacttc tgaatcaaat ggaatgagca tttttttctt taaattattt
     1261 ttctctcgtt tatttaaaat aagagggaca gaaacagcat cgtcaataat atattccttt
     1321 tcttttaatt ttaacaaaga aagcttattt tttatttcag aagatgaagt ttcttctata
     1381 tttgaaacga taagtgaaga atagacattt tttaatgttt catcatactt ttgatttatt
     1441 tcatcattta aatcttcttt ctcttttttt aataaaataa tatcattttt taacatagta
     1501 atttgtttat ttaaatctga tagtgtttct tccttactta atataaatct ttctctatca
     1561 tgtaatttat ttagtttttc atcatttttt tgttctaatt cataagcttc tttttttatt
     1621 gtgttagctt tcgacagttt agtgattcct ataattaata aaataagtcc aatcaataat
     1681 aacattcaaa actcctcatt tctttgatat aattattgta gagtttactc agaaatgagg
     1741 aaagagtcca tgtgaaagca tgggcttttt tatttttaat gttgtaatac taaatgttca
     1801 agattatgag gaagccctaa atattcaata atttggaact gactcatatc agataaatct
     1861 tgttcatcta ttgttctagt taacatttca attgcaaaca tattagcttc agcttctata
     1921 ccataaatca tacaactaag gttgtttgct ctaaaagctg ctgtgcaaat acctttatgg
     1981 agtctaacgt gggctatttc atgccataat acaaatcttt tcatagaagg tgataggttt
     2041 tgattaatta atatcacata acatctttta ttgtgtatcg tttgtcctaa tgttttattc
     2101 cctaaatcag tttcaattaa atctatatca gaatatttga ttattttata tggattaatc
     2161 gcatcagatc caaactctac aaataatttt tctatttctt ctaatatcca gtatttgttt
     2221 aaatgcatac caaagcccac ctattgacga tattttttag gagtgaattt tttctttgct
     2281 ttaactttgg ttaaggcaag tgcaagttga atggcatcgt ataatttttc cttatcttca
     2341 tcgtctaaag gttcaccata ataatttgtt tcagcatctg aattcaagcc ttcaagtaat
     2401 ttttcagctt gtaaagcaat atcttgttta tctttttctg ttaaatcata ataatggcgt
     2461 ttatcagtac gtcctaaaag ataatcagtt gatacattaa agtaatctgc aactttttgt
     2521 attttatctc cagatggagt acttttattc catctagaca tgcttccatt tccgaaattt
     2581 aactttcttt caagttctgc caacgtcatt gaacgttctt ttaataagat ttttatacgg
     2641 tctaatagca taggatatgc attcctttca aaataccgac aaaaaaagta tttttctgca
     2701 aattatctat ttacaaatag agaatttgca gttatactag ttttgtaagc aagatattta
     2761 cggcatacaa agactaaaag caataaacta atagtctccc cagacatcag taagctcgct
     2821 ttttgtgcgc tgtattttgt atgcttacat aatagcatat ttgcagtttt tgtcaaactt
     2881 attagacaaa aatattagaa aatattcgat aagggggtat gaagcatgac tttaaaacag
     2941 caagtattaa ttgctttagt aaagaaaggt tggagtcaaa gagagttagc tcgacgaatg
     3001 ggtatatcaa ttacttattt acgagatatc ttcatcgaaa aaagaaaacc taaggaacgt
     3061 ttaaaacaaa tcgaagaact tttggatatc aagttagaag tagattctaa tgatcagcca
     3121 tcagaacagg aggcgtaaaa atggtacaac gtttgagcaa agcggagttt aaagagaaat
     3181 ataagtattc tgaaagtact tatcaaagaa gaatgaagga atttaaaaaa tcacgtttta
     3241 gtgaggggta taacgcagtt acttccaaag aaataacaat tgatgtcgat ttatatgaac
     3301 agtttagaac atggcgttct aaaaatcgat ttagagttag agctgtttgt taatagctaa
     3361 aaaatctaaa ttcaccactt ctgaaaagag gagttgctgc tctcctttca agtttgccaa
     3421 agtccacgtt ttttcatttt ggcaactcct cttcttggag gtggtgacgg cgagagacga
     3481 aggagggatg acatgaagga agaatcaaaa atagggattt tgaatgagaa caattacaca
     3541 agattaatta ttgaaacaga agaagggaaa aaagtagcag aagttacatt gaccgatgca
     3601 actcctgctg acggttatcg tattcgatta actcctaaat atgattaacc ttttggtggg
     3661 tgtggatcgt ttccgtgact atcacggctt tgaatttttc catctctgcc atgaataaat
     3721 agctcagatc cttgattttt agaaatctct ctagcgattt ttactgcttc tgattttgta
     3781 ttagtatgtg cagtcgcttt agaatttcca gcacctttga cattccaacc accattggaa
     3841 ttaggtacaa catgttgatt ttttcccata gttttcacct ccatttctat agaaatattt
     3901 tatcaaatag aaatggagaa attcaataaa cgaaaggaga agtgatatgg catatacctt
     3961 gcaacaagaa catcaaattc tcggtttgat taaacaacgc aggaaacaat tacaagatga
     4021 ccgtgcagcg cttagaaaag ccgatgagct atcagatagg caagctgaac taattgcttc
     4081 tgaacttgag gatttgagaa tgctagaaat aaaaaatagg gagattagat tatgaagaag
     4141 acagacacac tttttatagg attcattttg gggttattag tgattgtagc gcaccaaagt
     4201 attatcgggg gaagcttgtt cgcagcattg atggttttaa tcaatctgct tgattcaaaa
     4261 gaaaggagca actatggcac gagaagaagc gctaaaaatc ggtaaagtga ttgctgataa
     4321 tcggtgggga aatagccgcc ctattatttt aagcaagcaa catatcgaaa agcaaaaagc
     4381 atggcaacaa ataaaaaagt gactctgcca gccagcaaag agtcacatac aaatttataa
     4441 aactaggaga attatatcac atgaataaga aaattgaaaa tcttatcgaa gaattgaaac
     4501 gtgaatgcca aaagcaaggt gtttctatta tttgtacagc ccaaaaagaa ggggaactta
     4561 aaagtctcgt ttatggtgag acaacagaaa ttttactttg tcttgctatg caagaagaac
     4621 atttagatga aaatctccca ttgtcagcac atattatgcg tagaattgct gtggatgctt
     4681 acgaacaagc gaaaaatgaa gaagaaaatc aaccttcaaa tcatactttt gttattaata
     4741 acaaggaaga tttagctgat gtgatgactc gtattctcaa gggggaattt caatgatgaa
     4801 tgaaaaaaca attgatctac tcattatttt atttttcacg ttagtgattg tagcgaaaag
     4861 ttatgtaatc tttggcttgt tattcgcaat taccatgctt gtgctttgcg tgttagcaag
     4921 cagaaaagag gttaaacatg aacgagttag caaatcttga caactattta actgatcctg
     4981 attatgctaa accaccatat gaagcaccaa ttgatgagga ggatgaagat gagtaaatct
     5041 accttagaaa tgagccatca agaatggctt gaagaccgta agaaaggcat cggaggttcg
     5101 gatgttggaa caattttagg attgaataaa tggaaatctc cttatcaact atggctagaa
     5161 aaaacaggac aagtagtact tgaagaatca ggaagtgagc ccgcttattg gggtaatgtt
     5221 ttagaagaag tggttgctaa agagtttcag gaacgtacag gcaaaaaagt acgcagaaga
     5281 aaccaagtat ttgaacatcc attacatcca tttttaagag caaatattga tcgggaagta
     5341 gtgggagaaa atgccattct ggaatgcaaa acagccaatc aatttctcgg taaagaatgg
     5401 gaaggcgaag aggtaccgct tagctatctc tgtcaagttc aacattacat gaacgttcta
     5461 aacaaagact attgttacat cgctgtctta atcggtggtc aaaaatttat ctggaagcgg
     5521 attgaacgag atcaagagct gatcgataca atcactgaac aattagtaga gttttgggaa
     5581 acaaacgttc ttggaggtat cgagccaatt attgatggta gtcaagcgac agctgatttc
     5641 ttgaaagaga agtatgcaga tgttgaagac gttcaaacga cgttaccaat tcatttcgat
     5701 gaactagttg aacagaaaaa cgaactcaaa cggactaaga aagaaattga atcagctatc
     5761 cgtcaagtag acaacgagat tatcagcgag ctaggtaaac gtgaagccag tatcggtatc
     5821 actcaaaaaa acatcatcag ttggaaattt gtccgtacga gacgtatgaa ctcgaagaaa
     5881 ctagcagaga aatatccaga tgtcgcaaat gatgaagaga tttataacgt tactgaatca
     5941 agaaggctaa ccgaaaggga gatcaaataa tatggcaaca aatgaatcgt taaaaaatca
     6001 attggcagca aagccacaga aacaagttgc accaggtcaa ttaggactta aagctttgat
     6061 gaatacacca acaatgagaa agaagttcga agaggtgctt catgacaatg ctaatgcttt
     6121 tatgtcgaat gttatgactc ttgtatctaa tgacagttat cttgcagata gtgaaccgat
     6181 gtctatcatg agtggtgcgt taactgctgc aacattaaat cttgggctag ataagaattt
     6241 aggttatgca tatttagttc cattcaatag taaaaacaag caaacaggaa aatgggaaaa
     6301 gaaagctcaa ttcatgcttg gctataaagg atatatccaa ttagcccaac gatcaggtaa
     6361 atacaaagca ttaaatgtga tcgaagttta cgaaggagaa ctaaaaagct ggaaccgact
     6421 gacagaagag tttgagtttg atccaaatgg tagaacgtct gatgaagtca ttggatatgt
     6481 tggctatttc gagttactga atggattcaa aaaaactgtc tattggacca aacaagaaat
     6541 cgaagctcat cggattgcta acaataaaga tcgcgataag acaaagttaa gtggtgtgtg
     6601 ggcatctgat tacaatgcaa tggcacgaaa aactgttttg agaaatcttc tttctaaatg
     6661 gggaatcttg tccatcgaaa tgcaagaagc caccacatcg gatgagagag tccaaagggt
     6721 tcaagaagac ggcagcatta ttgctgaaac agaagttgaa gaagatattc ctgaaagaaa
     6781 agaagcagag gttatatctg aagaaaatga agatgtacaa actggattat ttgatgcatc
     6841 taatccgccg ttaaacaaat aatgagggag ctttctccct tgcttttcta gaggagaaat
     6901 acgaatgaac acaggatatg tcaaattaca tcgaaaagtg atgaattcat tcgtttggac
     6961 caatccttat atgtataaat tatggaactt gtgtttgatg aaagcgagtc atgagaatcg
     7021 caagatactt tttaatggaa aagagataca agtgaacagc ggagaattcg tcacagggcg
     7081 cgatgctatc acatctgaga tgaacgaagg cgtgaaacgt gaacatcaag tgaacagcgg
     7141 ttctgtatgg agatggctaa aacaatttga aaaaaacgga atgttgaaca tcaaatcaac
     7201 tacgaaatac agcgttatat caataaaaaa ctggtctttg taccaaagca gtgaacaaca
     7261 aatgaacatc aaacgcacaa caagtgaaca acaagtgcac acaaacaaga atgaaaagaa
     7321 ttataagaat gaaaagaata ataaaacatc atcgtcgcaa ccacgcaaaa agcgtgttta
     7381 cgacaccgac tcagtttact acattctcgc ggaggagtta ttcaaacaga tttgtcagaa
     7441 tcaggaaatc aaaaagccga atctgcaaag ttgggcagat gacattcgga aaatgatcga
     7501 gatcgacaag cggaccgaaa accaggtacg gggaatgatt gaatggagtc agcacaatgt
     7561 gttttgggca tcgaatattt tatctgctaa aaaattgcga gaaaaatacg acacaatggc
     7621 agcgcaagca aatcgtgatt ataaaacaaa acaaactaaa acgcttgaat acgagaaatt
     7681 tagcacagat gagttgccta tttgagagga ggcgtaggca tggaaactgt tggggaaatc
     7741 atggaaaagc tgatacagaa agtgcttgtc caacgtggcg aatgtcctga atgcggacag
     7801 cctttgtatg gatggcgtac gaagaaccct gatggctcag aacgttgtaa gccaacctgt
     7861 atgcaatgtg ggtataaagc attgcgagtt caggaagact tacaaacaga acgaatttac
     7921 aacgagagcc tgaaagcaag agcaatcaat tttttcaaag gtggttctgt tgtgcctaat
     7981 caagcgttgt ttgattgcac attgcagaat tatcaaattg tcgatcaaga aacaagacaa
     8041 gcggttgaag taaccaaacg ctttgttaat tcagtcttgt taggaaatcc aagtcacctt
     8101 gttttaactg gaaaacaagg aacaggtaaa agtcacctag cgatggcagc ggcttgggaa
     8161 gtcttgaagc gatcaaacta cgataaaaaa atcttattta tcgggttaca ggaaatgctg
     8221 gatcaaatca aattttcgta caacaatcct gaactcagaa aaacgattga gggatcgttg
     8281 attgcagata tcaaaacagc ggatttagtc atcattgatg atatcggttc agaactagga
     8341 aaagatgcat cagatagtcg agcgtttggc ataaacacgc taaattcgtt cttggatgca
     8401 cgacagaacc tagcaacgat tatcacaacg aacttgcttg gtgaagaact gaaaaaagct
     8461 tacggtacga gaacaatatc aagaatgttt gtcaactctg atggatttac gatggtattt
     8521 tctcaaacag cagacaagcg cataaaacca gtcaaaggag cgatcgcatg aatttgacta
     8581 aacaagaaaa aagaatctgt cgtcagataa tgttgcaaat tataggtgat cgtgacatgg
     8641 acgaattatc tcaacaagag agtcgaatgg ttcggaagat aagtaagcta attatttatc
     8701 gggatacaga aagatttctc actgttttag gtaacgtcta cgagaatcca gagttattgg
     8761 aggtagagta attgccaaaa atattagatg catgttgcgg tagtagattg ttctggtttg
     8821 ataagaacaa ttctgacgta acatttatgg attgtcgtca gcagtatgaa gaattatcaa
     8881 caggtcatgt gatcaatgtt gatccagatg tggtcgcaga ctttcgcgat atgccttttg
     8941 aagatgatgt gttcgatatg gttgtatttg acccgccaca cttaagacat gtcggcgaaa
     9001 actcatggct tgccaaaaaa tatggaaaat tagacgaact ttggccagaa gacatcaggc
     9061 aaggttttgc agagtgtatg cgagttttac gaccttcagg aacattaatt tttaagtgga
     9121 acgaggaaca gataccactt tctgatgtgt tagaggctat tggagaacag ccattgtttg
     9181 gtaataaacg aagcaaaaca cattggcttg tgtttatgaa aaaattcagc taacggagga
     9241 aacaaaggag gaaaagtaat gaagattgta ttaaataaat gttttggtgg ctttgggttg
     9301 agccatgaag caaaaatgga aatttttaaa agaaaaaata ttgaagtatt cccatacatc
     9361 aataacttta gctatgactc agatgatgaa tacacgagat acacaggcca aaagctaggt
     9421 tcgatggatt ttatctacta tttcaaaaaa gacccgaaaa tcgacaaagt tacaggaatt
     9481 tatagcgaaa ttgaccgact gtacggaatt gctgatgatt ccagtttttc aagtgattca
     9541 aacagaggtg ataaggacct cgttgctgtt gtcgaaaaac taggaagtga ggccagtgga
     9601 ccatatgcaa gtcttaaagt cgttgatata cccgatgggg ctgagtggga aataagtgat
     9661 tatgacggcg ttgaaactgc acattatggt tttcaaacag gtagtattta gtccactaat
     9721 gacaactaaa gaggaggcag aaggatgagt gaagatgaat actttgaaca gtagtctatt
     9781 aacgacagag atttttaact ggaaaggggt gtagtcatgg acgcatcgga agtagtaaat
     9841 aaaattaata gtctcaaaga ggttttcgga gacgtcgaag tagtgataaa cgcacaaggc
     9901 tatgacaacg aattttttaa gcagattacc gacattagca ttcagcaagg aatggaagac
     9961 gaagacggaa actttatcga tgaagtagcc attttagtga cttgcgagta agtaattgac
    10021 agctaaagag gaggaagcgg aatgaaacta aaagacggat tttactccag cggccacggc
    10081 atcggcggtt taatgctaga tatgccgaca aagaatccta aaacacgtaa gaaaccaaaa
    10141 ttcaaagtcg gtgacatggt tcgctgcgaa gcagaaggat tcatctatcc atttcgtgga
    10201 tatgtagaac acgtctataa tcactcagca atcattcgca ttgaaaacac gatggaatgt
    10261 gataagtggt tagcgaaaag caaagagaat ttagcagtgg tgagattggt ggacatggaa
    10321 gttataaaat cttaattttg cttttttaca actatcctac aacaatgagc gttattccac
    10381 aactggaagg agaagaggca attgtgaaca ttttagagaa tatagatatt aagcaaacaa
    10441 ggaaaaatgc tagacgatta ctaaaaagat acagaaattt agaacgtttg gtcggaccag
    10501 tgaaaataga cttttccgtg atgactgtta ccaaaaattt gaaattcaca attgacagtc
    10561 aaaacgaaga aatcattgaa gcgataagta ctcgagattc ggttatcgaa gcattaacac
    10621 ggctaagtag aatccatttc caggtacttt attatagtta ttgttttcct aataagatgt
    10681 caatgtatca aataggagaa aaattaggat attccgatag aactatcgag agaatgaagg
    10741 cagtagcttt ggttgaattt gctgaggcgt ataaatcagg agaactcatt tcacgtacaa
    10801 aataaaaaaa gtcggattcc tccgactatg aataatattt ccgacataag tattatatca
    10861 taattggggg aatcagagga tggtactttt cgatgtaaag aaatatgaga caccaagcgc
    10921 gaaagatgtt gatatggaac gcacaaaaca taatgtcgct gtgtttcttt cagcatatct
    10981 atcagctaga tgtagagtag ggcaacatcg agaaccaaaa gtgacagcat cttattcctt
    11041 ggttccacct tctacagctg atcatatatt tgaagcagaa agaatgctga tcgataaaga
    11101 agaagcacag gaagaatttg agtatctgca taaattgttt gttcgaggct attcagcgat
    11161 acagcatccg cataagcctg atgtgactga aaggcgcaag aagatattct atgatcgtta
    11221 tatcaatggt ctgcccattt atgtaactgc tcaaaggaat aatactagcg aagaatcggt
    11281 taaagtagaa tcaaacagaa ttatcatcca atttgcttca tcgttagaac tggttgcttt
    11341 caagtagcca gtttttacac tttttatacc cttttattac caatttggtt tccattttat
    11401 acctttttta taccaatcac ttacctattc aacgttgtat tatgatagtg tcgaaaaatt
    11461 aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
    11521 gcttcgatag gcagcaacgg aaatattaag aataaggatg tgaatttcaa ctccttctaa
    11581 attgttctta ttatctatca tccgttgctg tctattgttt ttttaattat tcacacgata
    11641 actaaaggta agtgaagaga aatgattcca ttaataattt caatttttgc gctttgtctt
    11701 aatgtatata tgattggatt taaaaatggg caaaataaaa aatagtagca gccaagaata
    11761 attttatagt gtcactgtgg cggaaagggt agacgctaag catgtgtgct aggtcaatgc
    11821 ttcggcaacc atgcaaggtt cgattccttg ccagtgactt tggcaaccga ggcatcggcg
    11881 gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg tgcatcgtgt aggttgctat
    11941 tacataactg gttaggttag attgagattt gggattcggt acaaatgaat cgtcaaatgt
    12001 ctcaagcaca ggatcggaaa cgtccctgcc tgtgcattac atattagatc actctttgag
    12061 tggtcttttt ctttacataa aggaggctac ataatgagaa actactggta tgtatcgcta
    12121 actaatgaat atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa
    12181 atcaaaggga agtactctat tgtcgaaatg gggagagaag cagaaccatg tgagattgat
    12241 gcgtgcaagc tcgtttattg cggtcatggt ttctatgatg aaccaaacat tcaaaataat
    12301 attaacaaga atttgaggga ttagaatgca agaaatggct taaaaatctc caatcaggac
    12361 aaatcaagtt agacaaagtt tccgatttaa agatattgat tgaagctgat ttaatgctaa
    12421 aagatattga aaattagaaa acaaaactca acctaaacag aatgtgaggt ggtgtgtatt
    12481 gaatggcaag acaacgtgat ccaagacgtg atgaagccaa aagaatttgg ttagaatcca
    12541 acggagaaaa gcagttaaag gaaattgcat ctgaattaaa tgtttcagat tctcaggtta
    12601 gaaaatggaa atcgcaagac aaatggagcg ctgaattgat tagtaacgtt accaatggca
    12661 aaagtaacgt tactaatcaa ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca
    12721 aaggaaatag ccgagcttct ccgccagtgg gtaataaaaa cgctttgaaa acaggtgagt
    12781 atgaaacaat attttttgac acgttaaccg ataatgagaa ggacatctat tctagtttga
    12841 atgatgatcc ttcttttgct ttgtccgaag aaatacgact tcttaaaata agacaacttc
    12901 gaatgatgaa aagaatccaa caagctgaag ctggactaaa tgatgaagaa gtcgaacgat
    12961 tgcagcagct aagaaagatt aaaactccga ttgaaaaaaa tggtaaaaag ctagaaatca
    13021 agcgtgaggt tatgcaagat gtgcagatta gcagaaaaaa acatcgcaaa attgatgata
    13081 ttctttcaat tgaagattca ttgactcgga ttagcaacca gttagctaaa gccatcaagc
    13141 aaatgaatga actttatatg aatgaataca gaactgattt aattaaagct cagactgata
    13201 agatccaagc tgagacaaat gaaattggcg gaaataattc aggtgaagaa atagaagaat
    13261 ggaaacaggc agttttaaat gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa
    13321 tttattcctt ttgctgatat tggttctgcc attgattatt actatgataa accagtagct
    13381 ttttgccaag atattttgca tttgaatcct gatgaatggc aagaaaatgt tttaaatgat
    13441 ttagctgaat tttcaaaggt ttctgttcgt tctggtcaag gagttggaaa aacagcatta
    13501 gaagcaggag caatactttg gttcttaacg tgtcgaccct acgctaaagt aatagcaaca
    13561 gctccgacaa tgaagcaact ttacgatgta ctttgggcag aggtagctaa atggttaaat
    13621 gatagcttga tcaaaaactt actgaagtgg acaaagacca aaatttatat ggttggtgat
    13681 tcagagcgtt ggtttgctac ggctagaaca gcgactaaac cagaaagtat gcaaggtttt
    13741 cacgaggacc atatgttgat tgtggtagat gaagcttctg gtgtgtctga tccaattatg
    13801 gaagctattc ttggtacgct atcaggtttt gataataagc tgttgatgtg tggaaacccc
    13861 aataatattg aaggtgtctt ttatgattcc cataattcag accgtgataa atacagagtt
    13921 cataaagtat caagctatga tagtaaacgt acaaacaaag acaatataga aatgattctt
    13981 aaaaaatatg gaaaagaaag tgatgttgct cgtgtccgta tttttggaga atttccgaaa
    14041 ggtgcgttgg attcatttat cagtcttgaa acagttgaat tggctacaga aaaacaaatt
    14101 agtgattctt tagtcaataa aacaaaggtt gctcatattg gtgtcgacgt agctcgatat
    14161 ggtgatgatt ctacgattct ctttcctaga attgctacca gggcattgga gtatgagaag
    14221 tattcaaaac gtagcaccat ggaaacaaca ggatatgtca tcaacatggc caagaatcta
    14281 atgagtcaat atccgagtat tgataaagtg atgattaaag tcgatgacac tggtgtcgga
    14341 ggtggtgtaa ccgaccgcct agaagaactt atagaagaca aacattatcc ttttgaggtg
    14401 tttggagtga ataacggttc aacatcagaa gacgattttt acgataattt aggtactcaa
    14461 ctatggggaa acatcaagga aatgttagaa gaaaatatga cagcaaatct taacggagaa
    14521 cagcctgtta ttgaattgcc ttctgatagt tcgttaatca aagaattaag tactcgcaaa
    14581 ttcaaaatga caagtagaag tcgtatacgt ttagaaagta aagatgatat gaaaaagcga
    14641 aatattggta gtcccgatat tgctgacgca ctggctttag cgttttatga gccaccaagt
    14701 cactatcaat ttattcaatt ttaggaggtg agcttttgat tagtacagta ttatctttag
    14761 agagatataa gaagctacgt gttaaatatg caacacaaat tgaagatgga atgtttgatc
    14821 cgaatggttt tatagaagat atgaaaccat tttttgctga tcgcgaaaga aaatatctag
    14881 cttatactag cgaaaagaat gaaatagata atagaccaaa gcctaacact gatattgtaa
    14941 aagttaataa taaacttcat gctggtatgt attcaattgt cgtagaccaa gcagtcaatc
    15001 attttactgg tatacctatc aaatgggatt acgatgtatc ggaacaaaaa agaacactca
    15061 ttcaaagatt aaaagataaa ttcttaaaaa atgatataga acttccgaca gttccagaag
    15121 cttttaacaa attaacaagc aatcttgatt ccatgagatt cgcaatgctt gattctgaaa
    15181 ctgcaacttt tcaaggagct tgtggagtag ctttccgatt gctagagcct gtggaagaag
    15241 atgatggttg gaagttaaga gcaagtaata ttgaaccttg gagagcagaa agatatggaa
    15301 atgccggaat ctatatcaaa gaaaagtatg actcttacca gaaaaaattt tttcaagaaa
    15361 tgaaagttat gacaagaaat aaaattctga catatgcttg ctatggtgat ttgaattttg
    15421 ttacaagcgg aacgttcaaa aaaattgatg agaccgataa ccctttgggg acgattacct
    15481 tggcagaatt taaaaataat acgaatcgtt attgtgattt tgaagtagct gaggaaattg
    15541 gagatgcaat tgatcgtgct ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg
    15601 cgtacatgct tgttactggc acaacaatga gcaaaggcac tgctaaagaa atgatgaatc
    15661 agctagggat tatcaactta aaagccccta ctgcaaaagc tgaatatgtc acaaaaaatt
    15721 tagcaaaaga tttcaatgaa tatcatatgg atctattgaa aaaacagttt tacaccattt
    15781 gtaaagcaat cgattttaac gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca
    15841 agtggcaaat cattagccta gaagcgaaaa caaatactaa agagcaatat tttagagaag
    15901 gattgaaaga atgcgcagaa acaattgctg cttttcttaa gtttcatgac aaagtagaaa
    15961 ttgagcctga aaaaattatt tttactttct ctagatcttt gccaactgat ttaagttatt
    16021 tagctgaagc tttacctaaa cttgcaccat acgtatccaa acgaactatt cagagccaaa
    16081 ttccatttgt aacagatgtt gattatgaaa atgaaatgat ggaattggaa agtggaagtg
    16141 cttatccaga tagtgaatac aattttggcg gaggtggcaa tagtgacgat agaaacgaag
    16201 tattggacca aacgtcgaga actggaggat caagcaaggc tcaaacaaga aaatcagaca
    16261 cttaaaaaat taactagtgt atttcctgaa gcactgaaag agatacaagc aaaactatta
    16321 tcacaagctg acttacacaa tatcacttat ccagaaatga tggagtttta cagtacaagt
    16381 aatcagaaaa aatatcgtga atatgtggaa aaaaattata agtcattaaa aatgtatgat
    16441 gcaaaataca aagagtttat cgatgaattt tttccaccat ttgactatgc aaaagtcaat
    16501 cgcttattac aaatacgatc agatgtattt aaaattcttg cagaatatgc gatggatgca
    16561 gatgtgaatc aatatttttc tgatcgctta gaggaaattc ttcaaagaac atattcttct
    16621 aatgctaatg tttttgttca acttttaaac gttgatatac caaattattt accagaaaat
    16681 gaaattaaac attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg
    16741 ggaaatattt catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt
    16801 ggagaaggcg ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg
    16861 tttaagttag aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat
    16921 gcaaaatttg cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac
    16981 aacgtattga ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag
    17041 aatccctata aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt
    17101 tgtagatgta cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat
    17161 gaccgtttat ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg
    17221 aaacggagga agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc
    17281 tgatattgca gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag
    17341 agaccattgg aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca
    17401 agataatact ccagttgata agaataccgt taatttaacg attcaattat tatctcattc
    17461 attaccaaat ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag
    17521 aactgactac aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg
    17581 ttaaactagc gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag
    17641 caatgcgggg cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc
    17701 attaatgcca atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga
    17761 tgacttcaaa tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc
    17821 tcaaagtgtt gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa
    17881 acaggatacc atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca
    17941 actggaaaaa ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa
    18001 taaagcattt gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc
    18061 tatttctcaa tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc
    18121 tgttgaaggt tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa
    18181 agaactacaa atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca
    18241 gaacaatgaa acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa
    18301 ctaggagagt gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt
    18361 tcaccagcga ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt
    18421 gctggaaaaa cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac
    18481 aacgatgatt cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga
    18541 acttggttta acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta
    18601 gatgctatta atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt
    18661 atggaaatga aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat
    18721 ggttctaaag gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca
    18781 gaaacagcca ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg
    18841 tatgaaaaag gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta
    18901 atgactgatg agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact
    18961 tttggattgc aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa
    19021 ccatcattgc caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca
    19081 ttacgtgaat ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt
    19141 gtaggtgaat actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag
    19201 tctgaataaa agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa
    19261 agatgaaact aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt
    19321 tgggtttgtt catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat
    19381 catttctata gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga
    19441 aatttaggaa gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa
    19501 gaagattttc cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta
    19561 gaaattgaat catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg
    19621 attagtgaag tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt
    19681 agacgtggcg atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt
    19741 ctaggctatg gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa
    19801 tgaatgaagc agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga
    19861 cggacattga agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata
    19921 atcctatttc ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg
    19981 gagagatggt aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac
    20041 taaaaaaagg agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat
    20101 ttgctactaa gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga
    20161 aaaatgggta atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat
    20221 atgactgtaa ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc
    20281 gttagtttag ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt
    20341 gtagtaaaag ctagaaaact atcaagcggt cgaattgttg gagaagttat aaacaatgaa
    20401 cattatttta aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg
    20461 aaattcatgt taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt
    20521 aaacaaatgg caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc
    20581 tgcaatcagt agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt
    20641 tatgcagtca actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa
    20701 tgtaggaatt gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa
    20761 ccctaataga aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat
    20821 tacaatcgat gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt
    20881 tcaacatatt cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat
    20941 gggagaaaaa agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt
    21001 ggaagatgag acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt
    21061 aagtatcaaa ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca
    21121 agatcaattt acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca
    21181 taaagtttta tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg
    21241 agaagatatt gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata
    21301 ccgttatacg gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat
    21361 tgaaggaagt aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc
    21421 aggacttgga tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat
    21481 cggaaattgg tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac
    21541 agaataatgt caaaatatca aacaacgatt aaattaacga aaaaagacga agaaggtaaa
    21601 tatgagcaag tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct
    21661 gcaggagtta tggaagaaat gcaaacagcg actgataagc aatcagttaa aaaagcttta
    21721 agtcgtgctt attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat
    21781 tattgcaaag gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct
    21841 gttactgcag gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca
    21901 agtcaccttc atttaagtat tcgattactt accgagaatt agatataaag acgcaattac
    21961 tcgaagcagg ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc
    22021 tttttgcttt tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca
    22081 ctcaatttta ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga
    22141 tgaatctggt tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga
    22201 aacaatcgaa agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt
    22261 tgctggaagt tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat
    22321 caagggttcc ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa
    22381 tttccaaaat atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct
    22441 tttatctcaa ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca
    22501 acttcttgct tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa
    22561 tgatggaatt cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt
    22621 atctcaaagt ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc
    22681 tcaacttgga cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct
    22741 gaaagaaggt ttatctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga
    22801 aatggatacc aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac
    22861 taagggaatt aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga
    22921 ggtaatagaa ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat
    22981 tattgataaa gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc
    23041 tacagcagta tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt
    23101 gaaattcatg atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca
    23161 acttaaaggt ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg
    23221 cgttggaaat tcattaaaga ttatgggggc aatagctgct gctaatccat ttgttttaat
    23281 tattggtgca gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga
    23341 gcttagaaac aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt
    23401 aaaaaatata aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa
    23461 tgcagttgag ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg
    23521 gaaaggtatt aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa
    23581 tgcagtcgat agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg
    23641 gcaaagtata aaagattcag ccagagaaaa atggaatgga atcaaaggtt ctattatgtc
    23701 agttgctggt ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct
    23761 agagacctta tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa
    23821 tgttatactt gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac
    23881 aaaaaataat atgattgctg tttggaataa tattaaagaa agtgccataa atatttggga
    23941 atctataaag aatatatttg taagttattt tacaaatatt tattttgctg cactcaatat
    24001 ttggacaggg ttcaagctta cattgataaa tatttggaat gaagtggtaa atcaagctaa
    24061 atcgatttgg ttaaacataa agtatttttt tattaatctt tggattgata taaaatattt
    24121 tgcaattcag aaatggattg aattgaagtt ttctattatt caaacttgga tcgatttaaa
    24181 atataatgcc attactactt ggaataatat taaacagttt ttcaaagata cttggaaaaa
    24241 tattaaagat acggcataca atacgtggat ttctataaaa aataccatga ttaatacatg
    24301 gaataacatt aaggactctt tctggaatat tgttactgga attgttaatt ctgctgaaaa
    24361 tgcatggaca aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa aagaaatctt
    24421 taattcgtta agggaagtca atttatttga aattggcaag aacatcattg atggacttat
    24481 aaatggtgta aaagaaaagt ggaatgcttt gaaagatact atcaaaggta ttgcaggtaa
    24541 tatcaaaaat tggattaaag gagctttaga tattcattct ccatcaagat ggatgcgtga
    24601 tatggttggt aaaaatattg ttcaaggtat tatcgttggt attgataaag agcaaagtaa
    24661 gttagatcaa accatgacaa atttagtaaa aacaccgtct gttcaacctg tagtaaatgg
    24721 aataagtcac ctaccaatta cacaaacaaa gcaaaatata tctgctagtg aaacaaaaga
    24781 aattcattta catttaaatg tttatggaga tttacctgat tcaatggttc ggaagattac
    24841 cacaaaaatt aaaacagaat tgacaagaca aatgaaacga gatgctggtg cagtaggagg
    24901 aacattatat gcaacttaag agaggacaat tttttattaa tcaacattat tcttctgaat
    24961 ttaatgtgta tattcaaaac aggcctgctt ctgtttcagc tagtcgagta attgaattga
    25021 gagagcgaga aggaaatgat tctatcatta ttgataaagg ctattacaaa aatgtgacta
    25081 gaaagattga gtgttactat aaagcacctt caattgatat ggtgcaggag tgggaagatc
    25141 gaatcactga atggttagat atgtcgtctt atagtgattt tattctttac tatgatgaac
    25201 aatatattta tcaagctgta gtaatagaag gacctgaatt taaaggaaca agaaaaacag
    25261 gaaatattgt cccttttgaa tttacagtga gtattcgacc atttaaggaa aattacaatg
    25321 gtagatttac tattcgacaa acagaaactt ttgaaattta taacccagag aagtattctt
    25381 caaaaccgct tattaaattg agtggttctg gagatgcttc tttttatatt aataaggata
    25441 aatatgattt gaaatcgttg gacagagaat tatttataga ttctaaatta gaagaggctt
    25501 atcgaaaatt agatggtaat ttagaacatc aggatcaggt caccttattt ttagattttc
    25561 catttttata tcctggaaaa aatgaaatta aatggacgaa aaatattcat tcatttgaaa
    25621 taatgcctag gtggtggaga aaagtatgaa accaagaata tatagtccta ctgaaacaga
    25681 tttctcaacg aatggcttag gaattttaaa ggattgtaca agatgtggaa tatatgaagt
    25741 agctaatgga aaatatgaat tagagttgga ttatccttta ggaactagat ttgatgaata
    25801 ttttgaaaat gactatcaaa taaaagcaaa gccaaacgat caagaagagt atcatatctt
    25861 ttttattgat gataaagata tagatacttt tttaaataca gtaactattt atgctcagag
    25921 tcgtacaaat cgacttggaa gacgggtagt cactcttgtg gaagtggact ctaaaactgg
    25981 tcaagaagcg atatcaatta ttgaaactaa aatggataaa aaatctgaca tacgacttta
    26041 ttctgatatt acggctgttt ctagcacaat ctttgaagcg agaaatgttt taaattgtat
    26101 tgctggtgaa caaggatcat tgcttcaata ttggggcgga gaaattaaac gtgaaccatt
    26161 taagctttct ttgttaaagc gaagaggtcg caataatgtt ggaacgattc ggtatggaaa
    26221 agatatgtct ggtttgaagg tcaaattaga ttggacaggt ataaaaacaa gaattattcc
    26281 atatgctgat cctcaaagtg aagtaggtac gactaatcga atttatggtt cgccagtaga
    26341 tagtgcctat attaataact atcctgatgt ctatacagaa catgttcagt ttacagaaga
    26401 acaaggagta aaagacatta agagcttaaa taaaatagct aaaaattact ttaaaacgat
    26461 caatcctggc tgtgataagc ctaaagtttc tattacggtt gaatttgata agttgactga
    26521 tagtgaagaa gcgaaagaat ttgcgaagat tagaaattat ggtctgtttg atacatttaa
    26581 aatatatcat aaaaaatata atatttatct tgaatcaaag gttagtggac tacaatacga
    26641 ttctttatct gaaaaaactt tgaaattaga agcaggagac attcaagttg ctttttatca
    26701 gcaacaagct gtaactattc aggataaatt aaaagattat gcaacgaata attatatgag
    26761 tgattttaat gattatgttt cttcaatgat tacaggtcaa ggcagtgcag gaggatatgt
    26821 agttttatgg ccaaaagaaa aaccttccaa catctttatt atggataatc cagatttaga
    26881 aaaggcaaga gaagtactaa gaatgaataa aaatgggatt gctttttcta agaaaggatg
    26941 gcgggggcct tttaattcag cttggacatt agatagtata tttaatgcca attttataca
    27001 aactggatta atcaaagcag atatctttca aaattctttt aacaagactg gagatgtatt
    27061 gaaattagtt aatggactac tccaaatttg gaacaacaaa aagaaaataa tggagttaac
    27121 taaaaaagga atggaatttt ggaattctaa gggttcaatt ggaacgattg gaacaactga
    27181 ttctgttggt aatccttttc ctggggcttc tactccaacc cctattgaag ataattcttt
    27241 agttattcgt acaaatggag acggcaaata tattttgatt tctcctaaag taggaaaagg
    27301 attagtttta ttaggaaatg gtaaagcaat ttattttgga gacttagatg tacaaggaaa
    27361 acttacagtt aaaggaaaag aaataattcc tggacaaggt ggtggtccat cgggaggtgg
    27421 agaatctact ggtgggtatc ctagtgaatt aaaaacagat gcagaaaaga gagcttggag
    27481 aat
//
LOCUS       DAZKPO010000035        27186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000035 DAZKPO010000000
VERSION     DAZKPO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.035"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..1628)
                     /locus_tag="ACG8NZ_001427"
     CDS             complement(60..1628)
                     /locus_tag="ACG8NZ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0539106.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1632..2042)
                     /locus_tag="ACG8NZ_001428"
     CDS             complement(1632..2042)
                     /locus_tag="ACG8NZ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0539107.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2039..2962)
                     /locus_tag="ACG8NZ_001429"
     CDS             complement(2039..2962)
                     /locus_tag="ACG8NZ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0539108.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2959..3837)
                     /locus_tag="ACG8NZ_001430"
     CDS             complement(2959..3837)
                     /locus_tag="ACG8NZ_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0539109.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3824..5161)
                     /locus_tag="ACG8NZ_001431"
     CDS             complement(3824..5161)
                     /locus_tag="ACG8NZ_001431"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0539110.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5164..6267)
                     /locus_tag="ACG8NZ_001432"
     CDS             complement(5164..6267)
                     /locus_tag="ACG8NZ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0539111.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6341..7849)
                     /locus_tag="ACG8NZ_001433"
     CDS             complement(6341..7849)
                     /locus_tag="ACG8NZ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0539112.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7815..8507)
                     /locus_tag="ACG8NZ_001434"
     CDS             complement(7815..8507)
                     /locus_tag="ACG8NZ_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0539113.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8529..9503)
                     /locus_tag="ACG8NZ_001435"
     CDS             complement(8529..9503)
                     /locus_tag="ACG8NZ_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539114.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9803..10675
                     /locus_tag="ACG8NZ_001436"
     CDS             9803..10675
                     /locus_tag="ACG8NZ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539115.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11133..13331)
                     /locus_tag="ACG8NZ_001437"
     CDS             complement(11133..13331)
                     /locus_tag="ACG8NZ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539116.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13563..14555
                     /locus_tag="ACG8NZ_001438"
     CDS             13563..14555
                     /locus_tag="ACG8NZ_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539117.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14615..16036
                     /locus_tag="ACG8NZ_001439"
     CDS             14615..16036
                     /locus_tag="ACG8NZ_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0539118.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16029..17528
                     /locus_tag="ACG8NZ_001440"
     CDS             16029..17528
                     /locus_tag="ACG8NZ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0539119.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17567..18961
                     /gene="uxaC"
                     /locus_tag="ACG8NZ_001441"
     CDS             17567..18961
                     /gene="uxaC"
                     /locus_tag="ACG8NZ_001441"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0539120.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19120..19803
                     /locus_tag="ACG8NZ_001442"
     CDS             19120..19803
                     /locus_tag="ACG8NZ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0539121.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19805..20641
                     /locus_tag="ACG8NZ_001443"
     CDS             19805..20641
                     /locus_tag="ACG8NZ_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0539122.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20652..22406
                     /locus_tag="ACG8NZ_001444"
     CDS             20652..22406
                     /locus_tag="ACG8NZ_001444"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539123.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22682..23023)
                     /locus_tag="ACG8NZ_001445"
     CDS             complement(22682..23023)
                     /locus_tag="ACG8NZ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0539124.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23091..25262)
                     /locus_tag="ACG8NZ_001446"
     CDS             complement(23091..25262)
                     /locus_tag="ACG8NZ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0539125.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25473..26330
                     /locus_tag="ACG8NZ_001447"
     CDS             25473..26330
                     /locus_tag="ACG8NZ_001447"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0539126.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26401..>27186)
                     /locus_tag="ACG8NZ_001448"
     CDS             complement(26401..>27186)
                     /locus_tag="ACG8NZ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0539127.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         8075 a         5610 c         4885 g         8616 t
ORIGIN      
        1 aaaaaaattc actaactagt gaaaataagt tagtgaacct catttattaa agaattaaat
       61 tacagatgat cttttccatc atacttgaaa aactcaaaat cagcataact tcctcttttt
      121 ttatttagat catgcactgc tatcccgata aaatttccag taaaccctcc tgagagtata
      181 cacatgtttt tttgcggacc tactttcttc cattcttcag ttgatacgtc tttcaaatga
      241 aactgtgctg tctctttctt tacagtgact ttcaaaaaag tcttcttttc tgtgagtgga
      301 atcgtccaat cttctatttg tgctactccg ttttctgatt ctatcatacg taggcatttt
      361 cccttgtcct cattccaagt aacataacaa taaatataat tggattcatt taagtacagt
      421 accagtccag ccatctgatt aaaattatta ggcatgtatt caaatgctgt ctccgcatca
      481 aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt cgaaggtaga ttgaagagat
      541 tcaccggaaa tcagacgtaa aaaacctggt ctacattttg tatcacacca atcagcattt
      601 ggcaaaatgc gtctggcatt ccagttcttc tttagctctc cttcaaatgt atccataaac
      661 tgatgatctt tttcttgttg tacctcttca tttgtctcaa tcattatttc ttccatcgga
      721 gttgtctggc tatctttaag tcttaaccaa tcgtcggacg tccaatacac ttcttgaata
      781 gcggtttctc ttcccaaaat agccgcaccg tttaatggtc tggtacaaag atatgtcatg
      841 taccatcttc ctgacggggt ttgtactaag ctcccatgtc ctgtacattg caatgtagaa
      901 gcaggtttgt cactagctgt cagcatagga aaaccaggat caagctcgta aggaccagta
      961 atattctttg ctcggcaaac tgtaactgaa tgtcctcttc ctgtaccgcc ttctgccgtc
     1021 atcaaataat aatacccgtt acgaaaatac aaatgtggtg cctccgtttt cgctaattct
     1081 gtacctgcaa atattttata gatgggacca tcaagcttct tcttctctgc atcatattct
     1141 tgtaatacga taccgctgga tttattcggt gtgtccagac gataatccca tagttcattg
     1201 acaagccatt tttttcccgt ttcaagatca tggaacaaag aaggatcaaa gccgttacta
     1261 tttaaataaa ttggttcgga ccaaggacct gtgatctcag atgcagtgat caaataattg
     1321 tggacatctt taaacggacg gcctgcactt ttgacatctg tgtaaacaag ataaaacaat
     1381 ccatcagcat aactgatctg tggcgcccat atacttccat ttgtaggatt tccttgcaaa
     1441 gatacttgag tggtcaaaat atccgttgta tgtgaccagt tgactaaatt tttcgactca
     1501 tatactcgga ttcctggaag ccattcaaaa gaggagacaa taatgtaata ccgctctttc
     1561 actcttaata tattaggatc tggattgaat cctcttaaaa taggattttt cactttgata
     1621 gtctgcatga ttcaggctcc ttttctcatt cttactcgat ataaactgaa tgccccagct
     1681 gcgaaataaa aaaatgcaaa aggaaacgca ataaactgag tgccaatcaa caatatgaat
     1741 cctgttataa aatacaaacc agtggcagtt gttaagtatt ttcctttagc agtcaaaaac
     1801 aatgctactg ctaaaaaagc aagtgaaata aacaacacaa cggcaaacag accagtcatg
     1861 tcctgtaata atccaaatgt attttctaaa gacaaagttg aatgatctac agcaagcgac
     1921 tgataacttt ttttaaactc tgacgcagac atattactta aagtaactgc taacccgcca
     1981 aagaaaatca aagatagact gccaccgacc attcctagca atagttccca ttttcttttc
     2041 atttctgaac cccctgcttt tctaaatgta tccattcagg tcttttgatc gatgtttcat
     2101 aattgttatt acgttcagag aatttgacag tctgttcatt tttcttgtca tcccaattat
     2161 cccaaccagt tgggtgaaca tgcccaccta tcttacacgc ttcaaataag acatttgcat
     2221 acggacgcca cggtcttcct aaatagacgt ttctagtatc aggttctgca gtcaaggtac
     2281 agctttcaaa acgataccct gttttttgat ttttcggcgt agatgctgct gtgatgtatc
     2341 caggattttt actggctaag ctatggatat gacactgatg aaaaacggca tctgccccac
     2401 caaatatgaa atccaccgtt ccttcaatcc agcaatattc ataggtctga cgacaatact
     2461 caggattggc agaagccggt gtacaaaaag gagtaccatc tttttgcaaa tcaggcaacg
     2521 gacccgtgca taaggtatcc tgataaccag atagtcgaca gttttttaaa gatacgcgat
     2581 ctcctgaatt gaataaagca acagcctgtc ctaccttttc tcctggacct gctgtatttt
     2641 cgatgtgaag attttctaat acgacattcg atccttctaa gaaaaaagta gcacttcgga
     2701 aagtccctaa ctggctaccg tcaagatact cctgttttgc aaaacgatta ccggagataa
     2761 ctactgttcc tgctcccaca agtttaaaat tggataatct gctttctata tattcatcat
     2821 atcttccttc ttttactatc aagacctttg gatggctatc cggcaggcct gccaaataat
     2881 cgattccttc ttggatagtt tcaaaatccg tcggggtatc tatagcattc tttccaatta
     2941 tcagtttgtc tttcatcctc ataaatgctc ccttatgaat tgtttccaaa tatttctcat
     3001 ttcttgtgtt tccatatgac cgcctgttaa agaaaaagaa gaaaagtttt ctggactacc
     3061 cattgattga tactttttgt ccagcccttt tctcagcttc agtacgcctt cgtccaagca
     3121 catcgtatcg ttttttccaa ctaagctaag acgagggcgc ggagcgattt tttcttggat
     3181 tgctaacgtt gtataagctg tcaatagatt tggcacatag taataaaacc catgatgatc
     3241 taggcggcga tgtagcaaaa gtatttcgat gtccacctgt gccgctagat caatacaaac
     3301 tttgacacga gaatccaatg ctgtcaccca ccagcttaat aagcctccca tagaaagacc
     3361 aatcgttgcg agccgttgag tatctgtgtc ttcccttgtc tccaaataat cgatcaagct
     3421 gatcacatca taaatacgca ttccccataa cgtttttcct gttaataaaa attgtttgaa
     3481 caactcgctt tcagaaatac ctccgcgttc ttcaaatccc caagcatcaa tcgaccaaac
     3541 agcatatccc attcctgtca gtacttctgc aaaagatgga gaagctaagt aaggagcacc
     3601 atggatcaat tcacttttcc cgactgaaaa atctccgccg tgagaatgca ggtaaaggac
     3661 cggcggaaac ggtccttctt tttctggata agcatagagg gctggtactg tttccaattc
     3721 atttaagtac aattgatagt attcaagcat aaaaccgttt tgtttttctc ggtataatcg
     3781 ttttccacta ggggtatgtc tgctagatgg agcacctaaa aaatcattca actgcataag
     3841 caaagttttt acgaacaacc agttcatcca tattttctga ttgatttgat tggaatttct
     3901 caatactgat ttctttagta ttttctaata gaaatgctgg tccctcgtga ttttcaatcg
     3961 acatctggtt aaaggaaacc ttacccgcaa agccaatata aaatccttgt ctgctcattt
     4021 cctctattcc tgtcatcata gcaggaacac cgggaactgc atcttgggcc atcgagacat
     4081 cgatattgtc aaatgtgatt tcttgtatga attgttccgc taatccataa ataaaacctg
     4141 cagaagcatg gacattcctt gccgtgatat tcgaaaaatg gatccttctg aatgctggtg
     4201 tccgttcatc tatcggataa gccttttttt cccatacata cggttctttc cctcgcggac
     4261 cacagaaata ataaagattc agtataaatg gacacatcac attatccatg actatattgg
     4321 atactcgaat atcttcaacg atcccgcctc gacctcgtct agatttcaat cgtattcctc
     4381 gatcagtttc ttggaagata caatttgaaa tggtaatatt acgaatacta ccactcattt
     4441 cacttcctaa tacgacaccg ccatgtccat ggaccatcgt gcaatttgtg atcgtgatat
     4501 tttcgcaagc gatcctttcg tacgtatctt cagtccctgc cttgattgca atacagtcat
     4561 ctcccacgtc aatatggcaa ttactaatac gcacattttt gcatgattct ggatcaattc
     4621 cgtctgtatt tggtgaatcc gctgggttca agatcgtcaa gttatcaaac gttgcattac
     4681 tgcaaagaat cgggttgatc gtccaacttg gtgactggat caatttaata tctttcacag
     4741 taattcgatg acagttatgg aaactcatca atttaggacg aggataagcg agattatctg
     4801 gttcatttct aaaagtatgc caccattttt ttccatttcc atctaatgta ccgaaaccag
     4861 tgacagagat gttctctaca ttttgagcat agatacagga agcataaact tttcgatgga
     4921 ctccttccca acgtgaatga acaacaggat aatcttttgg atcgtcagag aatttcagaa
     4981 ccgctcctgc gcttaaatga agttcgacat tgctttttaa aaataaagct cctgttagaa
     5041 attcacctgc tggaacaact acggtcccgc ctccgtcact tgctgcagca tcaatggctt
     5101 gttgtattgc ttcagtattc aattcatcaa ttgatgcgcc aaatgtcaaa atatcataca
     5161 tatttataat gcctccactt caatcaatgc ttgcaagaat gcaccaacgc ctttttgatc
     5221 attacaaata attggttcac tgatgtagta agcatatgaa ccatctcgct gatctgctcc
     5281 gcctaaccct gctacttgac agtttttatt caagttcagc cagccttctt ttgtttccag
     5341 aacaaattca tcaagtaatc cttgatgtga tttcttcact tgtggaagcc attcttcttt
     5401 gttcagcagt cccaaacgta ttcctttagc cattgcgtaa acaaacatac tactgccgga
     5461 agcttccaga taatttcctt tccgttttgt ttctgttgtt acttgatacc atgtcccgct
     5521 gttttcttct tgatatggaa gtaatgcagc cagtgtcttt tctagaattt ctttcagcaa
     5581 agaacaatct gtttcttcat gaagtaattc ataggtatct gctgcagcca tcaaatacca
     5641 gccaatcgat cggctccaaa aatgatggga tagacctgtt tttggatcag cccatggttg
     5701 gaccttcttt tcatcccatg catgaatcag caaatttgtt tttggatcga atagatgttt
     5761 ataactgatt tcaaactgtc tcaaaacatc ttggtaatct ttttcttcac taaaattctg
     5821 gataaattca gcataaaaag gagaacccat atataagcca tccaaccaaa tttgataagg
     5881 ataaatttct ttatgccaaa aagctccttc ggatgtgcgt ggatggtttg ccaactgctg
     5941 ggaaaggagt tctgctgctt ttttatattt ttcttcgcct gtttctttgt acaataaaaa
     6001 gaaaagcttt cctgtattta catggtcgat attgtattct tcaacgctgt agccacggat
     6061 cgttccatct tcttgcacga aatagtcgat attgtctttt atgaaatcaa aataaatttt
     6121 ttcttgggtt cgttcccaaa gtaattggaa ccctttaagt acgacgccat agtcataaga
     6181 ccattttcct ttataccact tttcttcata taagcgaggt gttctttcca tgatactttt
     6241 tgctaatttc tttgaccagt tttccattat ttgctctcct ttaaaaatta aaataggatt
     6301 tgggacaaat accaagaagt atttgtccca atcgtctata ttactgtgcc gctgctttgt
     6361 atgcttcctc gtactcttgt gtgattcgat cgcctccttg tgctttccag tcttctacag
     6421 cttttttgaa tccatcttcg tcaagctgtc ccatgatgta ttgcaccgta gcatcaatga
     6481 cgattttttc aatttctgat cctacttcat tgttcgtcgg tgaatccaat ggcacagaag
     6541 gatccattac agcaaattct tcattttctt tgatcaattg attagagaat tctttttcag
     6601 ggtctgtatc tttgaaagag aacgtcactt cactaggacg agagcttgat aacggttgca
     6661 cctcttgttg ccaaagatcc atatcgataa attcgataga accatcatca ttcaattcat
     6721 aatgcgttcc ttcgataccg ccagtcatca atttatacac atcttcttct aaaagatcat
     6781 caacaaattg aagtaatttc ttcaattgtg cttcgtcttt tacttgagat ttgggaaatg
     6841 ctagcaagcc gccaatacca tttccttctg accaaacatg ataaccgccg ccttcaccat
     6901 ctgagatctt gtttacaggc actaattcca tatcatcttg aataccttga cccatcgtct
     6961 tcagattctt gatatccacc attccagtgt agattccagt tcttccttgt gcaaattgtt
     7021 cttgctgatc agtcttttgg gtaactgcaa agtcttgtgc caaatagcca ttctcaaata
     7081 gatctcttga aaaattcaaa gctttcatat actcttctgt atcaaattct ggagtgaatt
     7141 tgccattatc gtctacagcc cagccatttg gtgctccaaa ataagaagtg aaaagtttaa
     7201 agctggtata acgaatatca ctaggcgcac gatcgccaaa accaactgtg tcgtccacac
     7261 catttccatc aggatcgttt tctgtaaatt gtttagctac ttcataaagc tcatccatgg
     7321 tagttggaac atctagtcct agattatcca accagtcttt tcgaatgact aagccagcac
     7381 gagcgtagtc tttttgtatc ggtacgccgt acaaggcacc gtcgatactc gcagcttcga
     7441 ttctatcttc agaaatttga cttaaatttt tatagtcttt taaatattct gatacatccc
     7501 agaatacgcc tgattttaac gcattgcgga cagaagagtt tttcaacatc gcaatttgaa
     7561 gtgtaacgat atctcctagt tcatcagaag ccaatgcagt tgttaatctt tcttcttttg
     7621 aagcatcagg aatccagttg aatgtcagtt ccgtatcggt tttttcttcg atcaagttca
     7681 caatcgtgtc tgtaggcggt gaagcatgat gcaaaatatt taaccaagtg attttagctt
     7741 ttccatcttc tgtctctgaa gaactgttac ttgaactgct gccgcatgcg gcaagtgcta
     7801 gaattcctgc aaaactaaga cctaatttta cgagcgttgc ttttttcata aattttctcc
     7861 ttcactgttt taataatatt tttgttttct tctgtttttt ctgtatatga gttcttccat
     7921 tccaaatggc aaaatataga atttgctgtc cacgtgactg cggaaaagaa gagactgcca
     7981 ccgataaagg ttaaaacacc tggcattgtt tggatgatca tcagataaat ccctaccata
     8041 cttaatatca acactaatga gtaatgaaga tgtgagaatc cgaataaaaa tgccattttc
     8101 attttgaaaa aatatccttt ccaatcataa tgagccatga ttgggaaaat atagagtgct
     8161 gagagcacat atatgaacgc agcaatatac aaagcgatcc taagatacca attgtgtatg
     8221 aaagaaagat ccacaagtaa aacaatcccg atggcgccat atatccagct catcagcaag
     8281 gtttcacgat agttttcttt aaaatatgtg aagtacgttt tcgtcaaagg aaacgtttct
     8341 tttgagcgca gccattttct aatcactgca accatcgcat acgatgcagg accgaaagta
     8401 aaaactccca aacctgcgat gactaaaccg ccccacagaa aattcaaata aaccaactgt
     8461 aaaatacgat ttagccttcc gtttatttta tctaatgact gcagcatatc tttccctctc
     8521 tttctatctt agtaaacgcc gtcttctcct agctttttgg caatcttatt agctaaaatt
     8581 accataaaca atcctacgac ccctttaaac aatcccattg ctgtactgaa acttaactgt
     8641 ccattcttca atccagctgt ataaatatag gtgtcgaaaa tttctgcaac gcttcgattc
     8701 aatgaattca ataacaagta catatgctcg aaccctaagt ctaatgtctg accgattttt
     8761 agaatcagca aggtaatgat tactggacga attgccggca atgtaacatg ccatgttttg
     8821 cgtaagcgtc ctgctccatc catttctgca gcttcataca actgtgtatc gactgcagtt
     8881 atcgctgaaa ggtagatgat cgtagaccag cccaactctt tccagatcac ttgtccgata
     8941 tatactgtgc gtgtccattc aggggatgtt agaaaactga ttttttcaaa tcccattgtt
     9001 gcaaggaatt cattcaatac ccctccgtca acattcaaaa atacataagt aatcgaaaca
     9061 atgattaccc aagacatgaa gtgagggatg taaatgatcg tttgaatccc gtttttcaat
     9121 cgtttgttcg ttacttcatt taatagtaaa gacaaaatga ttggcatcgg aaaaaacacg
     9181 aagatattta atccgaacaa gatcaacgta tttcttaata atgtaaagaa tgtcggttct
     9241 gtaaacaaac gaatgaaatg tttgaaaccg acccaaggac ttcccattat tcctaaaaat
     9301 ggttgataat cttgaaaagc aataacgagt ccccccatag gaagatacct aaaaatcaag
     9361 aaataaagga ctcccggcag aatcatcaca tataaaaatt tattcgtttt gattcctgca
     9421 attatttttt ttcgtcttct gagcttttct tcttttttcg atagcgtcag ttgaaatggt
     9481 tcttgtttct ttacactttc cataagcccc tccttagttt tgtgaatgtt tgctgcttat
     9541 gacttcatga tagataacta tctgaattac gtatataatc atttttaccg taagtaaaaa
     9601 cgcttacata gcaatgattt ttgtgctata caaaacgata aagtgtattt tttcacaaat
     9661 aaactacgca tagcaaacct tctatttttt tctatctgac acttagcata ggaaatgatt
     9721 atatacgtaa tctttcaccg atgataaatt gaacatatag aaacagaaac atttaattta
     9781 ttttgaggag gagcctatct atatgaaaaa gaaagtctct ataggagaaa gaagttttac
     9841 acttttcaac aatacgtttc taattttgct agctttgatc tgtatcgttc cttttttaaa
     9901 tatcatcgct acatcttttg cttctacaca agaagtcgtc gcgaaaaagt ttatcctttt
     9961 tcctactacc ttttctttag acgcgtatcg ctacattctt tctacgccga ccatttttag
    10021 agcacttgct gtttcgattg gtgttacagg tgtcggaacg atcgtcagta tgtgtgcaac
    10081 ttcactgatg gcctacggtc tttccagaaa gtatttgttt ggtcgaggtt tcgtcaactt
    10141 tcttgttgta ttttctatgt tgttcagcgg ggggatgatt ccaacgttct tagttgttcg
    10201 ttctctaggg ctggtcaatt cttactgggc catgatcctg cctgtggctg ttaatgcaat
    10261 gaatatgatc attatgagaa actttttcca agccttgccg gatagtttgg aagaatcagc
    10321 aaaaatggat ggttgcacag attttggtgt atttttcaaa attatgctgc cattagcgtt
    10381 accgtctatt gctactatct cgctttttta tgctgttact tactggaaca cttatatgac
    10441 tgctattttg tatatcaacg attcatctaa atggccgata cagattcttt tacgccaaat
    10501 cgtcattgta tcaagtggga tgcaagcaga aagtagtgcc gttgatgtga ttccaccagc
    10561 acaaacagtt aaaatggctg ttatcgttat tgcaacagtg cctatgctga ttgtttatcc
    10621 ttttgttcag aaatattttg tcaaaggagc actcgtaggt tctgtcaaag ggtgattatg
    10681 tactgaacgc aaaatggtag ttgtgtcaga agtggtcagc ttcaagaaat aagaaggcat
    10741 ttacgaaaat tgcttcttaa atttttgtga aatttcggct tatttttcga agaatctact
    10801 cctggaacac cgtgtatcag aggttgtgaa agagacgttc agccttgagc gatgataaaa
    10861 actctcacaa acattgcgac gagtaaccca caggagcaat aagaagcgat tttgaaaaat
    10921 agcttcccat atttcacaaa attatgcctt attgtcgata gcctgtctct tgaacgccat
    10981 ttattcgtaa aaaagaactg tgacaagact tttgtcacag ttctttttgc gtgcttagcc
    11041 ttgaaattta actatccata aatacaaata ctagggttat tctaacttct tttattgctt
    11101 gaatcagtcc acaagaaaaa cagcctcttg attcacaaat atttttttcg atattctccc
    11161 ggcgtaatcg attccttctt tttaaagaaa cgaataaaat tttgcggatt tcgatattgc
    11221 aatctttctg caatctcttt tacggagagg ttagtctctt tcaaccattt ttttgctacc
    11281 tctaaacgat gattttgaac aaaatcacca aatttttctc ctgtttcctt tttaaataca
    11341 ttacttaaat agttagggtt ataatgcaac ctatcagcga tcacatctaa agacagatca
    11401 cgatcatatt ctgtgtagac gatatgcata atattctccg acaaattttt aaattcatga
    11461 tcagtgattg cttgagagca ttctgtgatt ggcagaacta attgttctaa aacaagtttt
    11521 tctagttctt tgggatgata attgttcagt gcttttacat aaatttgttt catgttatca
    11581 aagttttttg aatctactcc caataactgt cctaattgaa cgagttcatt gatcaagcgg
    11641 accgaaacta cttcccgact caaaggattt ttattttgtg caaacaatgc atcaatcaat
    11701 tcatgtacta aattcttcac ttcctgattt ccagaacgaa tggcttcaaa caatcgattt
    11761 tgttcttcta tcggatatct aataagtgtt ttttcatgag cagcaggaac gatttcctga
    11821 taaaaaataa ttgaacttgg accagtattt acacgataat acagggcttc ttttgcccga
    11881 tcaaccgatt gcttgctttc attcagcgtt tcgaaccggt tactgatgcc tatactgata
    11941 gttaaattca agtattcttt tacacgtttt tgaatgagtc ggcaatactc catgactttt
    12001 ttgcttgctt ctgcatcatc tttatccatt cgataaatag tggcttgcat ttcttcattc
    12061 aaaacgatcg gcagcattcg attttccttt ggcacaattt ccgtgatcat gttgttgatc
    12121 gctaaaagta gcaaatcacg ctccccaccg tgactatctt ctaacagatc gatttgaacc
    12181 aaagctgtat aatagcagtc attttgagaa ctataaccaa attgctgcaa ccgctgatct
    12241 agttctcgcc tttctactcg atttctaaat aaactaagta caaatagcgt ttccagctgt
    12301 ggcttttggg taactaagtg tgcacttaac gcttcttttt ctcctatgat ccgactgact
    12361 gattgagcga ccaaatctag ttctttccca ctgaatcctt ttgatttaag attcagacgt
    12421 tcctgtattt gagaaatcgg acgggcaaat ctgtccgaaa ataagtagga aagaagacca
    12481 atgagtccta ccagtgttaa agacactgca actagcccga ttctcagatt gcggatcatt
    12541 gcgccaattg cactagagtc aacttctaat ctgtaaatcc aattattata atctgatttc
    12601 agtactatat acgttttatc tttttctctt atcactttca gctgattgta atctagatcc
    12661 acgtccgtta atacagttgg ttgctcttta ttttttactg tctgagtagt aaaaaggttg
    12721 ccaacttcgt tttcaataga aagtatgtct tctccacttt caccaacaac ttgttctatt
    12781 gtatgtttct ctatcgtagc aattcccaat gcatattttt ctttttcaaa cattggcaaa
    12841 gaaatcacca tttcgattcc tttgtttttc ttttgccaaa ataaattatt tttgttattt
    12901 atgtacttct gttgatactt ttcaatttcc tcttcggtca gttgactcaa cgatccattg
    12961 attactcccc atttaccagc caaactgact aaatcataat tattatcatc cataacaatc
    13021 gtttctataa aatttagttc tttcctgatt tcagaataag tggagtaatc tgtatatgtc
    13081 agcggatgct tgaatatgtc cgagaaattt ttggtcgaac catacgtaga aaacgaatat
    13141 tcaatcgttc taattttcca ttcaacgtta gttttaactt gcctgagcaa attctttttg
    13201 tctgttaaat aactcgattc gatcgattca taggtattct ggtaaatgaa taaactaaat
    13261 cctatgacaa gtacggctcc taataaaaac atcggtaaaa atattttata aaatactgta
    13321 cttttcttca ttttcttcct ctcctccttt taaaataaaa ggctttcatt tttctgtaaa
    13381 attgatactc atctaattaa atgagcattc cccgctttca agaatagcat aattaaaaat
    13441 aaaaatccaa gtaattttta acgtgtgcaa tcttttcata tcaaagatct attgacttat
    13501 cgtagttatc gtaatatgat gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg
    13561 gaatggtaac gatcaaagat gtcgctaaag cagctggcgt ttctcatacg acagtttctc
    13621 gtgctttgaa taacagcagt ttaataaaaa atgaaacaaa acaaaaaata cgacaactag
    13681 ctaaggaaat gaattattcg ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat
    13741 atctaatcgg actatttttc tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag
    13801 attccattgc aggcatccat tcagtattag acacaaatta cagtttatct gttgaaagta
    13861 tcgatgttgt tgatttaaca gaaatcaacc tgcaacgcta cgatggactg atcgttatga
    13921 gtcaaagtga tgatgatcag cctttcatcg actatctcaa agaaaaagaa tttcctttcg
    13981 ttgttgtcaa tcgtcacatc aatgatccag cggttatcaa cgtcactgcc gatgatgcgg
    14041 ccggtgttat gcaggcagta gattacggta ttggatgcgg acatacgaag atcgcttata
    14101 ttggcggaaa gaaaaatttt cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc
    14161 taaaaaaagc gagcatccct gtcaatccag cctatttttt aacaggagat tacagcttag
    14221 aaagcggcct ccaaaacatg gaatacctat tatcactttc tgacctgcct actctggttt
    14281 tttgcggcaa cgacgacata gcgattggtg cattgagagc tgtacattta gctggcctcc
    14341 gtgttcccca gaacatttct ctgatcgggt tcgatgattc tccagtcgtc tcctatctca
    14401 atccgccgct taccaccgta cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc
    14461 ttttactaga tttgatcgaa ggcaggacga tcgaacaatc taaaattgag ctctccacct
    14521 tgttgatcct tcgtgagtcg atcaattact tgtaaactac aagtaatttt atataaaaat
    14581 gttaacgtgt gcataacaca gaaaggaagt ttatatgaaa aaactgacaa aagaattagt
    14641 gaaaaaaaac gaagcacctg taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg
    14701 atttattgat tggcagattc agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc
    14761 aatcattcaa cctttagaac aaggtcttgg aaatatgatg aaccagcaag ataatttata
    14821 tacggtcatt ttacaaggac tcagcaatgg cgaaatcgtt gatacttccg agattatcac
    14881 cgtagtagaa caaatgatca atccttatga tgaatgggat gcttatctat ctctagcaga
    14941 aaatgacgac ttggcctata tcgtctccaa tacaacagag gcaggaatcc aattcgtgga
    15001 gactgataat ttaacagata ctcccccaca gagcctcccg ggcaaactga cagccttttt
    15061 ataccgacgt ttcaaattaa acaaagctgg ctttaccatc attccttgcg aactgattga
    15121 cagaaacggc gaacgtctaa aagaaatcat tttacgctac gcagaaaaat gggatttaga
    15181 acctgctttt gttgagtggt tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg
    15241 catcgtccca ggttatcctc gcgaagatgc aggtgttttc aatcaacggc ataactatga
    15301 agatcaattg atggtcaaag cagaaccatt catgctttgg gtgatcgaag ggcctaaaga
    15361 attggagaat caactaccat tcaaacaggc tggcttgaat gtcatcatca cagatgatat
    15421 gactccttat cgtgaacgta aggtgcattt gcttaatgga ccgcatacag cgatggtccc
    15481 tcttgctttg ttggcggaat tggaaactgt agaagaagta atgaaagatc ctttattctc
    15541 cgcatatgtt gatcagttat tcaatctaga attgattcct atgctttctt tacctaaaga
    15601 tgaattagcc atttatgctg accaaatcaa agaacgtttc ttgaatcctt ttgcccatca
    15661 caaattagaa gcgatttcat taaatagtgt ttcaaagttc agcacccgtc tgcttccagt
    15721 gttcaagaag tatatcgaag aacaaaatca ggtaccaccg ttaatcactg tttcactagc
    15781 tgcgctactt cttatgtatc gaggcgatca agtaaagcca catgatgatg aaaagacgat
    15841 aagcgaattt actgatgctt ggtctgataa tggaacagct attcctcgat tattgcaaaa
    15901 cgctgcgctt tggggagaag atttgtctca aataccgaat gtgacagata cagttcaaga
    15961 aatagctgca aagatcgatc aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac
    16021 gatggtccat gcttgaaacc ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg
    16081 tcgtcgcatt aacccctatt ccaaaacaaa cacaactgac gattgatcag caaacgatca
    16141 ccactcttga agatatcccg caaggtcata agattgcatt gggtgatttg aaagctggag
    16201 acaacgtgat caaatatggc tatccaatcg gtcatgtgac gacagacgta actgccggtc
    16261 aatggctgca cactcataat gtaaaaacca atctttctgg tgaattggat tatcgatatg
    16321 aaaaagatgt ccatccaagt cattaccttt ttgagaaccg aacatttcaa ggttacttgc
    16381 gaaaaaatgg aaaagtaggg attcgtaatg atttgttttt agtacctacc gttgggtgtg
    16441 tgaatggcat tgctgaactg atcgtcaaac agttcaagga aaaacatcca gatctgggac
    16501 agtttgacca tattacgatt ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc
    16561 atcaaaatac tcgagaaatt ttagcggacg cagtaaatca tcccaatgcc ggaggagttt
    16621 tagtatttgg attaggttgt gagaacaata cagttcctga attcaaaaaa attttaggtg
    16681 aatatgatga agaacgagtg aaatttctag tggctcaaga tgtttacgat gagatcgaac
    16741 aaggtgtggc tctattagaa gagcttttaa cagcggcgga aaatgaccag cgcatatcag
    16801 tcccgctctc taagttgaat gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa
    16861 taactgctaa tcctttactt ggtgcatttt ctgactactt gattgctcaa ggaggcagca
    16921 cgatcttaac agaagtgcca gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg
    16981 aaaatgaaga agtttttgac tcaatcgttg acttgatcaa tgacttcaaa cagtatttct
    17041 tgtcttacgg ggaaccagtt tacgaaaacc catctcctgg taataaagca ggtgggatca
    17101 ctacattaga agataagtct ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg
    17161 atgtgctaca atatggcgaa aaaatcagaa aaccagggct tagtttattg caagctcccg
    17221 ggaatgatct tgtagctgcc tctgctcttg cttcatccga ttgccagttg gtcttgttta
    17281 ccacaggaag agggactccg tttggctcat atgtcccaac ggtcaaggta tctacgaata
    17341 caactctttt tgaacgcaaa ggtcattgga tggattttaa tgcaggcgtc ttattagaac
    17401 aaccaatgga agtaatctta gaatcgtttg ttcaaaaaat catcgctgta gctagtggag
    17461 aagaaacgaa aaatgaacaa aatgacgttc gagaaattgc catttttaaa aacggagtaa
    17521 cactttgatt ttcataaaac agtataaaaa gaaagaagga agatttatgt ttttatctga
    17581 tgatttttta ttaaaagacg attgggcgaa aaagctttac cattcctacg caaaaaacat
    17641 gcccattatt gattatcact gtcacttaga tccaaaagaa atttacgaaa acaacaattt
    17701 cagcaatctg acagaagctt ggttaggagg cgatcattac aaatggcgct tgatgcgagc
    17761 ttgcggcgtg cctgaagaaa aaatcacagg aaatgcttct gattttgaaa aatttttagc
    17821 ttggtgtcaa accgtaccaa aaattatcgg aaatccgctt tacagttggt cacatcttga
    17881 attgaagcgt ttcttcggta ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg
    17941 gacacaagca aatgaaattt tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa
    18001 atcgaatgta gaagtgattt gtacaacaga tgatcccgca gacgacttgc actatcatca
    18061 gttgcttgct catgaagaac cagatttgaa ggttctacct tcattccgtc cagataaagc
    18121 actcaatatt gaaaaagaag gatttaatgc atgggtcaca ctactggaag aagcagctga
    18181 ccaaacgatc accacctatc aagaactgat agaagtctta gggcagcgga tagaatactt
    18241 ccatcaacat ggatgtcgtg tttccgacca cgctttagac atcttacgct ataaagaagc
    18301 ggatgaaaca gtattagaag agatttttca aaaagcacga gctaacaaac tattatcttc
    18361 agatgaaatc gatgcttatc gtacagaaac actgatacat ttgacccact tctatcattc
    18421 tcataattgg acgatgcagc tgcacatcca tgcttatcgt aactgtaata cacaaatgtt
    18481 ggaaaaatta gggcccgata ccggctatga tggaatgaat gatctttctt tgactattcc
    18541 tctgcaaaaa ttattgaacc gtgcagaaga aacggatcag ttgccaaaga cgatccttta
    18601 ttcattgaat ccgaatgact atcctgcgat tttagctttg atgggctgtt tccaaaaaga
    18661 acagaatgga aaactgcagt taggttctgg atggtggtac aacgatactc gagctggtat
    18721 gcgcgatcaa ttgacacaat ttgctgatgg tagtgctttg gggaattttg tcggcatgct
    18781 gacagattca agaagtttcc tgtcctacac tagacatgaa tatttccgtc gtgttctttg
    18841 tgaattttta ggcgaaatgg tcatgcgagg agaagcacct gaagatgaac aacttttagg
    18901 aagtctagtt caagcgatta gttatacaaa tgctaaaaat tactttggct tttttgccta
    18961 attgattaat agtaacagta acgaatcaaa aacaggatag tgaaataacg gaatccgttt
    19021 atccacgttt tagagaagaa gacggaccaa atttgctccg tcttcttctc ttgctgttct
    19081 gcttcattgc ctataaacct ttatgaaaga agagataaca tgcctactat ccatattgcc
    19141 ggtgattcta ctgcggcaga aaaagatgaa aacaagcgtc ccgagacagg ttggggagaa
    19201 aaaatctctg cttatttttc acccgagatc cagatcaata accaagcaaa gaatggacgc
    19261 agcaccaaat catttatcca ggaaggaagg ttagcccgtc tagcttcaca gtttcagccg
    19321 aatgatttct tatttgtaca atttggacat aacgaccaga aaatccaaga agaaagaggt
    19381 acgcttccct tcggtagcta tttaaaaaat ctgcagatat tttttgaatg cgcaaaaaaa
    19441 gcgaacgtcc aaatggtctt actgacatct gtgacgcgac gagattactt agaaaacgga
    19501 acattgaatc cagatatact tggagactat ccaaaagcga tgcgtgcatt tgctgagaaa
    19561 caccatattc ctcttttaga tgtgttctca agatcacaag aattgttcca gaccttctca
    19621 aaagaagaga caaaaaaatt ctacctgcat cttatacctg atacttgtaa aaattaccca
    19681 gaaggactaa aagacaatac ccactttagt ccagaaggcg ctgataaagt tgcccgaata
    19741 attgttgaat tgataaaaga atcccgtctg cctcttgcta attatttaca aaaaggagtt
    19801 tgagatgttt ccattaaatt taggcatccg tgcccacgat ttagatgttc gaaatcgtga
    19861 agaaatcgtt caaaaagtat cacagcttca cttgtcccat atccaatttg caccacaaaa
    19921 agcatttcct gaacaactta aattaactga catgacactt gggagtgctg cctattttgg
    19981 tgaatacttc caaaaaaaca atatcgaact ttccatttta ggatgctata tcaacctatc
    20041 cagcaaaaat caagaagttc gtgcacaagc attggcaact ttcaaacagc aaattgctct
    20101 gtgctctgcc tatcaagcca agatgattgc tacggaaacc ggtagtgtct ctgtcggtta
    20161 cacaaaagaa aattttactg aagaagctta tcagatcgcc agaaattcta tcattgaatt
    20221 agtcgctcat gcagaaaatt ttggcgtaac tgttgctgtt gaagcaggga tcaatcatcc
    20281 aatctataac tatcagttag ctaaacgttt aatcgatgaa gtagcttcac caaatttaaa
    20341 aatcatttta gattgtgcga atttgatgca taaagaaaat catgctgatc aggaaaaagt
    20401 gatccgaggt gccttggaca atttacatgg ttatattaca gcgcttcatc tcaaagacta
    20461 tatcatggaa aatggaaaga tccgcatggt acctgttggc aagggctgtt tagatttccg
    20521 cccggtactg cattatatca aatatgagcg tcctctaatg tacgccactc ttgaagctac
    20581 tcctgagatt tatgttcctg aagcaataga acacttgcag tttatttacc aagattgtta
    20641 aggagaagat tatggaggaa cgaatacaga aaagttatca aaaatatcat gcaaatgctt
    20701 ttcagacatt gctgcatctt tatcagggaa attattggcg ttttgccatg tcaggactct
    20761 tttttattat caaacatctt ccatcttggt taatgccgat cgctattgcc aatgtaatca
    20821 atgcagcttc atcgaaagat tcttctcagt tgcaggccat ttatttcaac gcttttttga
    20881 tggcttgtct gattggtcaa aatatattga cgaattattt tcatgtaaaa taccatagca
    20941 cttcgatccg tcaagtagaa gctggactaa gagcgaccct tatcaagaaa atccaagaac
    21001 tatctattcc ttatcaaaaa gaattgcaat ctggacgaat ccagtcaaag atcatccgtg
    21061 atgtagaagc tatccaaacg ttgtcctctc aagtatttgt cagcggatta aatatcgctg
    21121 tcaatttatt cgtagcttta ggtatcacgc tttataaaag tcctgttgtt ttcatgtttt
    21181 tcttattgac tgttcctctc gccgcattcg ttactctctt tttccgtaaa aggatcagac
    21241 agtccaactc agacttcagg caagaaatgg aagagacttc tgcaaaagtg atcgaagtga
    21301 tcgaattagc accgattgct cgagctcatg ggttagaaaa acacgaaaca actcgcttaa
    21361 ataagcattt tactcaaatc tatgaaaaag gattccactt agatatcctg caatctgttt
    21421 tcggatcagt cagctgggct agttttcaga tatttcagct gatttgtttg attttcactg
    21481 ggatattagc tttacaaggg cgcatccaac ccggcgatgt cgtgatgtat cagacgtatt
    21541 tcacgacgat cgtcaattcc gtttctggaa tgatcacgct gattcctacg atttcaaaag
    21601 gcatggaatc tatcacttct attggcgaac tgttgattgc tggcgatgta gaaacttatc
    21661 acggtaagaa aaaaatccaa catttggatg gaaattttcg ttttgaggat gtttcttttc
    21721 tttatccgga ctcactcaaa ccgattatcg atcatttttc tctagaggtg aaagctggag
    21781 aaacaattgc ttttgttggg ccatctggct ctggaaaatc tacactcctt aacttattga
    21841 ttggatttat ccagccgact tcaggaaaaa tttttctaga tgatcagcct ttggatgaac
    21901 tggatatgcg aagtgtgcga aattatttag ccgttgttcc tcaaaccact ttgttgttct
    21961 ccgcttcgat caaagagaac atcacttatg gcttgaaaaa cgtatcaaaa gaacgtttag
    22021 atgaagtcat cgaagctgcg caactttctt cactgattga ccagcttcct gatggtctgg
    22081 ataccttagt aggcgaacat ggcaacaagc tgtctggtgg ccaaaagcaa cgtatctcca
    22141 ttgcacgagc gttgatacgt cagccaaaaa tcattttgct agatgaagca acctcagctt
    22201 tagataacca gtcagagaaa aaaatccagc aagccctgaa ttacctgaca gaaacaccca
    22261 ctacttttat tgtggctcat cggctttcaa cgatcaaaga ggcagataaa atcgttgtga
    22321 tcgatgaagg aaaaatcgtt gaaataggta cttacgaaaa actgttagct caaaaaggct
    22381 atttctaccg cttgaaaaat gaatgacaaa aaataggagc tatgacaaga cttttgtcgt
    22441 agctcctatt tctgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    22501 gtcatcaaac atgagaagct gttttttgaa attgttcctt gtaactcaga gttaaacggt
    22561 aggctcacga cctcttggta aatggtgttc aagagacagc ctatcggcaa taagaagcaa
    22621 ttttgtcatc gctcaaggcc gaacgtctct ttcacaccct ctgttgtttc ataaaatagt
    22681 tttattcgct gtaaaggtcg cgaactggtg tcatgatgat acggttcaag tcattgatga
    22741 ttgtactgaa gccttgctct ttttgcatca agtcattgat tacttcttct gattggattt
    22801 tcacagccat ttcttgtgct ttcattgcat cttcgtcgct gaactcttgt ccactcattt
    22861 gtttttcttg taattcgatt tgcatcgctt ggaagtcttt gaacagtttg tgtgcttctt
    22921 cgtttgcttg tactttctcg tacgcttctt tcaacgcttt gaattctggt aattcacgga
    22981 tctcacgttc gatttgattc gcgctatcgt agatgtttga catgttatgc cctcttttct
    23041 tttagtagtt actctctatt gtaacatggg gttattcgtt ttcaaagggg ctactctcct
    23101 tgatatttcc gccagaggtc tttagccgct tcaccaattc cctgaacgcc ttctttcacc
    23161 atatcgccga agtttttcag cccttctttt gcttgttcac catattgatt caagttatct
    23221 tgccaatttc cagactctcc attagaattt cctgtgtttc caacttgatc tgcagctacc
    23281 acttgtcctc ctgtggcata ggcatctgct acactaaatc ctgtttttga agtatacgga
    23341 aggatgcctt ctgccgctga tttgaagact ttcccgacaa catttccact tgtcccttcc
    23401 aaatagtgca gcttgctgga ttcttcgtaa cccatccatg tcgaaatcac tacgtctggc
    23461 gtgtaaccga tcatccattg gtcatttgct tttgtggcat cgaaattagt ttctgtggtt
    23521 cctgttttcc ctgctatcgt ataacctgca ggattcgcag cagccgctgt tccatttgaa
    23581 aatgtaccaa gaagcatgct ggtcatttca tcggctgttt cctttgatat gacctgtttt
    23641 ggttttttgt ttgtgttatc gacgatcacc gctccagtcg aatcaacgat tttcgtgata
    23701 agatgcggcg tatacatctg accatcattg gcaaatgcac tgtaagcacc cgccatgatc
    23761 aatggagata ctcctttttc cagtccacca agggctaagc cataatactt atcagatttt
    23821 gctaaaggca ggccgaattc ttctgcttta tcatatcctt tttgtaaacc gatctcatgc
    23881 aaaagccata cagtaggcaa attcaaactt tgtgcaacag cttgatacat cggtacttct
    23941 ccttggtatg tcccgtcaaa attatgcgca tcgtaataac tttgcggttc gtctttcaag
    24001 atactggaag gcttgtatcc agcttccagt gctggtgtat atacgcttaa tggtttgatc
    24061 gttgatcctg gtgaacgacg catctgtgta gcaaagctga atcctctaaa gacatgatct
    24121 cctcttcgtc ctactagtgc ttgtacacca cctgtattcg gatctaaagc tactgaagca
    24181 gattggacca tggcaccatc tgaagcattt gctgggaata atgcactgtt ttgataagta
    24241 gcttctaatt gcttttgata atcctgatct aaagctgtat agattttata gcctttattc
    24301 atgacatctt cttcatcaag accatactcg ttaactgctt catcgatcac tgcatcaaaa
    24361 taataaggat aacgataacc agaatctgaa ttatcgtatg tgtcattaag atacgcgctg
    24421 atatctgtct ggctttctgc ttgttcttgt tcttttgtga tttttccgtt ttcttccatt
    24481 acacttagta ctgtgttttt ccgatcattc gcattttttg ggtgatcgat cggattataa
    24541 ataccaggac ctttcagcat tcctgccaaa gtagcagcct cagacagcgt aacattcgtt
    24601 gcatctactc caaaatactt tctggaagcg tcttggacac cccatacccc gttgccaaaa
    24661 taggcattgt taagatacat ggtcaagatt tcttctttgc tgtacttctt ttcgatctcg
    24721 attgccaaaa ataattcctt cgcttttcga tcaaacgttt ggtctaatgt cagatacgca
    24781 tttttggcaa gctgctgagt gatcgtactt cctccgcccc ctgatgttcc gccagacaag
    24841 accattcgga ctgctgcacg ggcaatccct ttgatatcaa acccgtgatg ctcgtaaaaa
    24901 ttcctatctt ctgtcgagat tactgcatct tggatatagg gagaaatcgc gtttaattct
    24961 acatatgttc ctttttgggc gtacagtgtg ccagcttctt cgtttttctt atcataaatg
    25021 actgtcgatt ggcttaatcc tgattttaac gtttcgacat ttgcttgttt tgctaaaata
    25081 aagaggtaga tacttgtcac taagacacag atcaaaccaa tcagcaaaat caatttgttc
    25141 acatgatatt ttttccagat gcgctttcgc catcgatgga attgtccgag atatggtctg
    25201 atccaacgcc aaaaactgat caaagcagct tttatctttt ctaaaatgct ttgaaaatcc
    25261 atttgcttct tctcctgtct atttgattca atcgtcttat tttaccatag tgaaagagtg
    25321 ttttcttcct gcttaagttg tagttcaata tgttttttta cgttagtgca agtagagtaa
    25381 cataagaatg aacagaaaaa tgtaagtttt ttcttaaata tgttttaaaa atggtacaat
    25441 tagtctgcga ttgcactgaa aggagtacac ttgtgaaaat cacgatcact ttacctatga
    25501 accatcctga aacaacaatt cgcgacttgc ttgaaaagga atggttagtt cctagaaagg
    25561 ttcgacactt tttgcgtata cgtaaaaacg tttggatcaa tcaacaacct gctttgttcc
    25621 atcatgaagt aaagggcaat gaccagatca cattgttatt cgaagaaaca gattataatt
    25681 atcaagaagt tcaattagga gatgcgaagc acatccgtgt tttatatgaa gatgagcatt
    25741 tgattatagt ggataagcct gcaggaatga agactcatcc aaatgaaccg acagaaaaca
    25801 gtaccttact caatcattta gctgcttatc tcgacactaa acagcaacga ccttacgttg
    25861 tccaccgctt ggacaaagaa acaagcggtg ccgttttatt tgcaaaaaat ccatttgtct
    25921 tgccgatact tggaagaatg ttggaacaaa aaatgatcta tcggagatat caagccatcg
    25981 tatggggaga aattgccaaa gatatgacca tcaccaataa aattggacga gatcgccatg
    26041 atcggagaaa aagggtcgta gatgataaaa atggtcagag cgccttgaca catgtcactg
    26101 tcaacaaagt actttctgaa caaacacaag tctattgtgt tttggaaacc ggacgtacac
    26161 accagatcag agttcattta gctcataagg gacatccgat cgttggagac cctctctacc
    26221 aaaatagaac agctgaacga ctgatgctcc atgcattaga actgcacatg atccatccat
    26281 ttactcaaga aaaaatagtc ggtaaagctt tacctggatt atgggaatag aaataaaaaa
    26341 gaacagagtc ttatcagcag ataatgataa gactctgttc tttttgtgta atttgtctgc
    26401 ttacttttga tgttcgtgtt ctgctaacag caaactaccg ataatcccac tttcattcgg
    26461 cagtccccaa cgaacgatat attcacttac ttgaggtgtt tccatataac cacccatcaa
    26521 ttcaacaaat tgctgacgga ttttttgaag gagatgatcc tgattcatta ccccaccgcc
    26581 taaaataatc ttttcaggtg ctaacgtcaa cgtatagttg atcagtgctt gagcaatata
    26641 ataagcctga atagcccaaa caggatgatc ttgcggcaga tcttgccctt tgatccctgt
    26701 gcgtgcctca atagacggtc ctgctgctaa tccttctaaa caatctttat gataaggaca
    26761 agtaccttca taatggtcat ctggatggcg tttcacccaa atatgtccca tttcaggatg
    26821 tgcgatccct gaaaaaattt cttcattgat cacgacacct gcaccgattc ctgttcccac
    26881 ggtaaggtaa atacaactat tttttccttg tgctgctcct tgcttcaatt ccccatatgc
    26941 agcagcattg acatccgttg tccaagcagc aggaatattg tagcgttcct tgattgtgcc
    27001 aagaaaatcg aattgtgccc accctttttt gggagttgcc aaaacgtatc cgtaattatc
    27061 ttttgttttg tcgataccaa tcgggccgaa tgagccaatc ccgattccct tcaattcata
    27121 acgatcaaaa aattcaaata cctgatccaa tgtttcttct ggtgttgtgg tagggatact
    27181 taattt
//
LOCUS       DAZKPO010000036        26811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000036 DAZKPO010000000
VERSION     DAZKPO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.036"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8NZ_001449"
     CDS             complement(104..1336)
                     /locus_tag="ACG8NZ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0539128.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8NZ_001450"
     CDS             1591..2160
                     /locus_tag="ACG8NZ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0539129.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8NZ_001451"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8NZ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0539130.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8NZ_001452"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8NZ_001452"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0539131.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8NZ_001453"
     CDS             3732..4655
                     /locus_tag="ACG8NZ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0539132.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG8NZ_001454"
     CDS             4731..5870
                     /locus_tag="ACG8NZ_001454"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0539133.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG8NZ_001455"
     CDS             5863..6663
                     /locus_tag="ACG8NZ_001455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0539134.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG8NZ_001456"
     CDS             6663..7490
                     /locus_tag="ACG8NZ_001456"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0539135.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG8NZ_001457"
     CDS             7495..8202
                     /locus_tag="ACG8NZ_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0539136.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8NZ_001458"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8NZ_001458"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0539137.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8NZ_001459"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8NZ_001459"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0539138.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8NZ_001460"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8NZ_001460"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0539139.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8NZ_001461"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8NZ_001461"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0539140.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG8NZ_001462"
     CDS             11787..13820
                     /locus_tag="ACG8NZ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539141.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG8NZ_001463"
     CDS             13864..15144
                     /locus_tag="ACG8NZ_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0539142.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG8NZ_001464"
     CDS             15354..16160
                     /locus_tag="ACG8NZ_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539143.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG8NZ_001465"
     CDS             16172..17392
                     /locus_tag="ACG8NZ_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539144.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG8NZ_001466"
     CDS             17382..18968
                     /locus_tag="ACG8NZ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0539145.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG8NZ_001467"
     CDS             19007..21145
                     /locus_tag="ACG8NZ_001467"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0539146.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG8NZ_001468"
     CDS             complement(21513..22496)
                     /locus_tag="ACG8NZ_001468"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0539147.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG8NZ_001469"
     CDS             22874..24265
                     /locus_tag="ACG8NZ_001469"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0539148.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG8NZ_001470"
     CDS             24281..25321
                     /locus_tag="ACG8NZ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0539149.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACG8NZ_001471"
     CDS             25340..26425
                     /locus_tag="ACG8NZ_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0539150.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26811
                     /locus_tag="ACG8NZ_001472"
     CDS             26458..>26811
                     /locus_tag="ACG8NZ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0539151.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEF"
BASE COUNT         8600 a         4451 c         5444 g         8316 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttattttaaa aagaatagtt cagtggagtt t
//
LOCUS       DAZKPO010000037        26512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000037 DAZKPO010000000
VERSION     DAZKPO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.037"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG8NZ_001473"
     CDS             172..516
                     /locus_tag="ACG8NZ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0539152.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG8NZ_001474"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACG8NZ_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACG8NZ_001475"
     CDS             complement(1028..1378)
                     /locus_tag="ACG8NZ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539153.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG8NZ_001476"
     CDS             complement(1344..2675)
                     /locus_tag="ACG8NZ_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0539154.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG8NZ_001477"
     CDS             2931..3497
                     /locus_tag="ACG8NZ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0539155.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG8NZ_001478"
     tRNA            3642..3729
                     /locus_tag="ACG8NZ_001478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ACG8NZ_001479"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ACG8NZ_001479"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0539156.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ACG8NZ_001480"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ACG8NZ_001480"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0539157.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ACG8NZ_001481"
     CDS             complement(7452..7898)
                     /locus_tag="ACG8NZ_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0539158.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG8NZ_001482"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG8NZ_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0539159.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ACG8NZ_001483"
     CDS             8363..8881
                     /locus_tag="ACG8NZ_001483"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0539160.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ACG8NZ_001484"
     CDS             complement(8958..9914)
                     /locus_tag="ACG8NZ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0539161.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ACG8NZ_001485"
     CDS             10080..10886
                     /locus_tag="ACG8NZ_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539162.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ACG8NZ_001486"
     CDS             10883..11872
                     /locus_tag="ACG8NZ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0539163.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ACG8NZ_001487"
     CDS             11869..12873
                     /locus_tag="ACG8NZ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0539164.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ACG8NZ_001488"
     CDS             13005..13547
                     /locus_tag="ACG8NZ_001488"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0539165.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ACG8NZ_001489"
     CDS             13567..14265
                     /locus_tag="ACG8NZ_001489"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0539166.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ACG8NZ_001490"
     CDS             14286..14498
                     /locus_tag="ACG8NZ_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0539167.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ACG8NZ_001491"
     CDS             14498..15460
                     /locus_tag="ACG8NZ_001491"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0539168.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ACG8NZ_001492"
     CDS             15478..15873
                     /locus_tag="ACG8NZ_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0539169.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ACG8NZ_001493"
     CDS             16036..16401
                     /locus_tag="ACG8NZ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0539170.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ACG8NZ_001494"
     CDS             16398..18209
                     /locus_tag="ACG8NZ_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0539171.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="ACG8NZ_001495"
     CDS             complement(18271..18438)
                     /locus_tag="ACG8NZ_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0539172.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="ACG8NZ_001496"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="ACG8NZ_001496"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0539173.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="ACG8NZ_001497"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="ACG8NZ_001497"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0539174.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="ACG8NZ_001498"
     CDS             21195..22175
                     /locus_tag="ACG8NZ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539175.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22366..23088
                     /gene="tsaB"
                     /locus_tag="ACG8NZ_001499"
     CDS             22366..23088
                     /gene="tsaB"
                     /locus_tag="ACG8NZ_001499"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0539176.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="ACG8NZ_001500"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="ACG8NZ_001500"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0539177.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="ACG8NZ_001501"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="ACG8NZ_001501"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0539178.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="ACG8NZ_001502"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="ACG8NZ_001502"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0539179.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25707..26408)
                     /locus_tag="ACG8NZ_001503"
     CDS             complement(25707..26408)
                     /locus_tag="ACG8NZ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0539180.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8573 a         4541 c         5528 g         7870 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatatttatc
    25681 agggtcactg cttatttttg ttcgcgttat tctctcatag ctggagtagt agctgtctta
    25741 cagaaagtaa aacgcttttt gtcataatct aacagcccgg aatcacgtag ttgtttgatt
    25801 acttgtccag cagtttcacg tgtcgtgccg ctattcactg ctaattcttt taaggtgatc
    25861 ggataaggaa tggtgatatg accatctttt gttggtacgc ccaattcttc tttcaaaatg
    25921 aataaatttt gttttacccg gtcaaaggcg ctagaggtca atccgatttg gatacgcaat
    25981 tcatgtattt caactacttt agaaattttt tgtatcatat agatcaactg ttcgggattt
    26041 tttcgtgcta atttttcata gtaggaaaca ggaaggtaga atgtttcaat atcagtcatc
    26101 gcttgagctg tataagtgta ggtctcttct ccaaacattc ctgacatagg aaaaaggtta
    26161 tttgtattca catagtcata gtagtaataa gttgcacttt catcataacg ttctaaacga
    26221 acgagtcctt tggtcaagat atacaaccgt tgtctgggat caccatcgtc gaaaaggatc
    26281 tgtccttttt tataagaacg aagaacagta tgttccttga tctgttggaa ctcttcatcc
    26341 gagaaatgtt caaaatcagg ttgatatctg agttttgaaa agtgaaaatc gattttatta
    26401 acacgcatta aaaagtcctc tccttattgt tcactatatt tattgtcact ttttttaaag
    26461 aatttgtaaa gttttaacat accaacatta gaaacaagtt ttaatacttt ac
//
LOCUS       DAZKPO010000038        26084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000038 DAZKPO010000000
VERSION     DAZKPO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.038"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8NZ_001504"
     CDS             <1..1125
                     /locus_tag="ACG8NZ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0539181.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACG8NZ_001505"
     CDS             1474..2106
                     /locus_tag="ACG8NZ_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0539182.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="ACG8NZ_001506"
     CDS             2694..4133
                     /locus_tag="ACG8NZ_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0539183.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="ACG8NZ_001507"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="ACG8NZ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            4895..5110
                     /locus_tag="ACG8NZ_001508"
     CDS             4895..5110
                     /locus_tag="ACG8NZ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539184.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="ACG8NZ_001509"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="ACG8NZ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="ACG8NZ_001510"
     CDS             complement(5770..6681)
                     /locus_tag="ACG8NZ_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0539185.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="ACG8NZ_001511"
     CDS             complement(6700..7704)
                     /locus_tag="ACG8NZ_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0539186.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="ACG8NZ_001512"
     CDS             complement(7701..9824)
                     /locus_tag="ACG8NZ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0539187.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="ACG8NZ_001513"
     CDS             complement(9829..12276)
                     /locus_tag="ACG8NZ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0539188.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="ACG8NZ_001514"
     CDS             complement(12263..12652)
                     /locus_tag="ACG8NZ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0539189.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="ACG8NZ_001515"
     CDS             complement(12712..13215)
                     /locus_tag="ACG8NZ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0539190.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="ACG8NZ_001516"
     CDS             complement(13228..13452)
                     /locus_tag="ACG8NZ_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539191.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_001517"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_001517"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0539192.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16349)
                     /locus_tag="ACG8NZ_001518"
     CDS             complement(16212..16349)
                     /locus_tag="ACG8NZ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0539193.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG8NZ_001519"
     CDS             complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG8NZ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0539194.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="ACG8NZ_001520"
     CDS             complement(17789..18079)
                     /locus_tag="ACG8NZ_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539195.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG8NZ_001521"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG8NZ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19287..19451)
                     /locus_tag="ACG8NZ_001522"
     CDS             complement(19287..19451)
                     /locus_tag="ACG8NZ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539196.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19444..20118)
                     /locus_tag="ACG8NZ_001523"
     CDS             complement(19444..20118)
                     /locus_tag="ACG8NZ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0539197.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="ACG8NZ_001524"
     CDS             complement(20196..21536)
                     /locus_tag="ACG8NZ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0539198.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="ACG8NZ_001525"
     CDS             complement(21619..21954)
                     /locus_tag="ACG8NZ_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0539199.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="ACG8NZ_001526"
     CDS             complement(21964..22338)
                     /locus_tag="ACG8NZ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0539200.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="ACG8NZ_001527"
     CDS             complement(22351..22665)
                     /locus_tag="ACG8NZ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0539201.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="ACG8NZ_001528"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="ACG8NZ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0539202.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7508 a         5453 c         4558 g         8565 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aacagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacagg ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat
    26041 aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaa
//
LOCUS       DAZKPO010000039        25998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000039 DAZKPO010000000
VERSION     DAZKPO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.039"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8NZ_001529"
     CDS             complement(164..388)
                     /locus_tag="ACG8NZ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0539203.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8NZ_001530"
     CDS             complement(586..2166)
                     /locus_tag="ACG8NZ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0539204.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8NZ_001531"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8NZ_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0539205.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8NZ_001532"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8NZ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0539206.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8NZ_001533"
     CDS             5331..5765
                     /locus_tag="ACG8NZ_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539207.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8NZ_001534"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8NZ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0539208.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8NZ_001535"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8NZ_001535"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0539209.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8NZ_001536"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8NZ_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0539210.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8NZ_001537"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8NZ_001537"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0539211.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8NZ_001538"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8NZ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0539212.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8NZ_001539"
     CDS             11001..11471
                     /locus_tag="ACG8NZ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0539213.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8NZ_001540"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8NZ_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0539214.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8NZ_001541"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8NZ_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0539215.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8NZ_001542"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8NZ_001542"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0539216.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8NZ_001543"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8NZ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0539217.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8NZ_001544"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8NZ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0539218.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8NZ_001545"
     CDS             16164..16967
                     /locus_tag="ACG8NZ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0539219.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8NZ_001546"
     CDS             17145..18092
                     /locus_tag="ACG8NZ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0539220.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG8NZ_001547"
     CDS             complement(18160..18453)
                     /locus_tag="ACG8NZ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0539221.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG8NZ_001548"
     CDS             complement(18481..19146)
                     /locus_tag="ACG8NZ_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0539222.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG8NZ_001549"
     CDS             complement(19286..19918)
                     /locus_tag="ACG8NZ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0539223.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8NZ_001550"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8NZ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0539224.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8NZ_001551"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8NZ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0539225.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8NZ_001552"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8NZ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0539226.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8NZ_001553"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8NZ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0539227.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACG8NZ_001554"
     CDS             23883..25052
                     /locus_tag="ACG8NZ_001554"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0539228.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..>25998)
                     /locus_tag="ACG8NZ_001555"
     CDS             complement(25319..>25998)
                     /locus_tag="ACG8NZ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539229.1"
                     /translation="TKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQLE
                     KFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVP
                     IGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVT
                     VNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKL
                     KIRTSHE"
BASE COUNT         7859 a         5190 c         4162 g         8787 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agttccttga aaattcattt ctttcaccat gttaactact gatgaataat
    25801 ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa
    25861 atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac aatgttgaaa
    25921 atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt aaggcaaacc
    25981 atggaaatat ttttgtac
//
LOCUS       DAZKPO010000040        25324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000040 DAZKPO010000000
VERSION     DAZKPO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.040"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG8NZ_001556"
     CDS             237..1877
                     /locus_tag="ACG8NZ_001556"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0539230.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG8NZ_001557"
     CDS             2031..3353
                     /locus_tag="ACG8NZ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0539231.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG8NZ_001558"
     CDS             complement(3415..3846)
                     /locus_tag="ACG8NZ_001558"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539232.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG8NZ_001559"
     CDS             complement(3910..4746)
                     /locus_tag="ACG8NZ_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0539233.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG8NZ_001560"
     CDS             complement(4936..5238)
                     /locus_tag="ACG8NZ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0539234.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8NZ_001561"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8NZ_001561"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0539235.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG8NZ_001562"
     CDS             complement(5692..5958)
                     /locus_tag="ACG8NZ_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0539236.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG8NZ_001563"
     CDS             complement(6212..6937)
                     /locus_tag="ACG8NZ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0539237.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG8NZ_001564"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG8NZ_001564"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG8NZ_001565"
     CDS             7490..8242
                     /locus_tag="ACG8NZ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0539238.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG8NZ_001566"
     CDS             8258..8737
                     /locus_tag="ACG8NZ_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0539239.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG8NZ_001567"
     CDS             complement(8988..10568)
                     /locus_tag="ACG8NZ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0539240.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG8NZ_001568"
     CDS             complement(10561..11304)
                     /locus_tag="ACG8NZ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539241.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACG8NZ_001569"
     CDS             complement(11480..12256)
                     /locus_tag="ACG8NZ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0539242.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACG8NZ_001570"
     CDS             complement(12504..13340)
                     /locus_tag="ACG8NZ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0539243.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACG8NZ_001571"
     CDS             complement(13351..14280)
                     /locus_tag="ACG8NZ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0539244.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACG8NZ_001572"
     CDS             14472..14825
                     /locus_tag="ACG8NZ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0539245.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACG8NZ_001573"
     CDS             complement(14899..15555)
                     /locus_tag="ACG8NZ_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0539246.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACG8NZ_001574"
     CDS             15781..17715
                     /locus_tag="ACG8NZ_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0539247.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACG8NZ_001575"
     CDS             complement(17801..19024)
                     /locus_tag="ACG8NZ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0539248.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACG8NZ_001576"
     CDS             complement(19018..20889)
                     /locus_tag="ACG8NZ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0539249.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACG8NZ_001577"
     CDS             complement(20910..22028)
                     /locus_tag="ACG8NZ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0539250.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACG8NZ_001578"
     CDS             complement(22111..22944)
                     /locus_tag="ACG8NZ_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539251.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACG8NZ_001579"
     CDS             complement(22937..23854)
                     /locus_tag="ACG8NZ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539252.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACG8NZ_001580"
     CDS             complement(24081..25205)
                     /locus_tag="ACG8NZ_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0539253.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7808 a         5048 c         4308 g         8160 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttc
//
LOCUS       DAZKPO010000041        24551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000041 DAZKPO010000000
VERSION     DAZKPO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.041"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8NZ_001581"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8NZ_001581"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0539254.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG8NZ_001582"
     CDS             complement(937..1518)
                     /locus_tag="ACG8NZ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0539255.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8NZ_001583"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8NZ_001583"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0539256.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8NZ_001584"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8NZ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0539257.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8NZ_001585"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8NZ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0539258.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8NZ_001586"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8NZ_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0539259.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8NZ_001587"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8NZ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0539260.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8NZ_001588"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8NZ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0539261.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8NZ_001589"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8NZ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0539262.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8NZ_001590"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8NZ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0539263.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8NZ_001591"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8NZ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0539264.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8NZ_001592"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8NZ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0539265.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8NZ_001593"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8NZ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0539266.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8NZ_001594"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8NZ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0539267.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8NZ_001595"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8NZ_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0539268.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8NZ_001596"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8NZ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0539269.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8NZ_001597"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8NZ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0539270.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8NZ_001598"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8NZ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0539271.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8NZ_001599"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8NZ_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0539272.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8NZ_001600"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8NZ_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0539273.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8NZ_001601"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8NZ_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0539274.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG8NZ_001602"
     CDS             13972..14157
                     /locus_tag="ACG8NZ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0539275.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8NZ_001603"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8NZ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0539276.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8NZ_001604"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8NZ_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0539277.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8NZ_001605"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8NZ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0539278.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8NZ_001606"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8NZ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0539279.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8NZ_001607"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8NZ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0539280.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8NZ_001608"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8NZ_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0539281.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG8NZ_001609"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG8NZ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0539282.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG8NZ_001610"
     CDS             18232..18879
                     /locus_tag="ACG8NZ_001610"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0539283.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG8NZ_001611"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG8NZ_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0539284.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8NZ_001612"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8NZ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0539285.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8NZ_001613"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8NZ_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0539286.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8NZ_001614"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8NZ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0539287.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG8NZ_001615"
     CDS             20317..21255
                     /locus_tag="ACG8NZ_001615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0539288.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8NZ_001616"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8NZ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0539289.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG8NZ_001617"
     CDS             21935..22774
                     /locus_tag="ACG8NZ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0539290.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG8NZ_001618"
     CDS             22750..23619
                     /locus_tag="ACG8NZ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0539291.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG8NZ_001619"
     CDS             23609..24406
                     /locus_tag="ACG8NZ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0539292.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         7953 a         4548 c         5279 g         6771 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa a
//
LOCUS       DAZKPO010000042        24455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000042 DAZKPO010000000
VERSION     DAZKPO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.042"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..850)
                     /locus_tag="ACG8NZ_001620"
     CDS             complement(2..850)
                     /locus_tag="ACG8NZ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0539293.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1074..3014)
                     /gene="thrS"
                     /locus_tag="ACG8NZ_001621"
     CDS             complement(1074..3014)
                     /gene="thrS"
                     /locus_tag="ACG8NZ_001621"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0539294.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3351..3530)
                     /locus_tag="ACG8NZ_001622"
     CDS             complement(3351..3530)
                     /locus_tag="ACG8NZ_001622"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0539295.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3577..5400)
                     /locus_tag="ACG8NZ_001623"
     CDS             complement(3577..5400)
                     /locus_tag="ACG8NZ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0539296.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5678..7957
                     /gene="helD"
                     /locus_tag="ACG8NZ_001624"
     CDS             5678..7957
                     /gene="helD"
                     /locus_tag="ACG8NZ_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0539297.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8050..8697)
                     /locus_tag="ACG8NZ_001625"
     CDS             complement(8050..8697)
                     /locus_tag="ACG8NZ_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539298.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8876..9595)
                     /locus_tag="ACG8NZ_001626"
     CDS             complement(8876..9595)
                     /locus_tag="ACG8NZ_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539299.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9726..10877)
                     /locus_tag="ACG8NZ_001627"
     CDS             complement(9726..10877)
                     /locus_tag="ACG8NZ_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0539300.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11094..11834)
                     /locus_tag="ACG8NZ_001628"
     CDS             complement(11094..11834)
                     /locus_tag="ACG8NZ_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0539301.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11838..12161)
                     /gene="rsfS"
                     /locus_tag="ACG8NZ_001629"
     CDS             complement(11838..12161)
                     /gene="rsfS"
                     /locus_tag="ACG8NZ_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0539302.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12218..12811)
                     /gene="yqeK"
                     /locus_tag="ACG8NZ_001630"
     CDS             complement(12218..12811)
                     /gene="yqeK"
                     /locus_tag="ACG8NZ_001630"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0539303.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12795..13445)
                     /locus_tag="ACG8NZ_001631"
     CDS             complement(12795..13445)
                     /locus_tag="ACG8NZ_001631"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0539304.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13455..13766)
                     /gene="yhbY"
                     /locus_tag="ACG8NZ_001632"
     CDS             complement(13455..13766)
                     /gene="yhbY"
                     /locus_tag="ACG8NZ_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0539305.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13782..14891)
                     /gene="yqeH"
                     /locus_tag="ACG8NZ_001633"
     CDS             complement(13782..14891)
                     /gene="yqeH"
                     /locus_tag="ACG8NZ_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0539306.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14888..15415)
                     /locus_tag="ACG8NZ_001634"
     CDS             complement(14888..15415)
                     /locus_tag="ACG8NZ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0539307.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15595..16380)
                     /locus_tag="ACG8NZ_001635"
     CDS             complement(15595..16380)
                     /locus_tag="ACG8NZ_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0539308.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16373..17242)
                     /gene="accD"
                     /locus_tag="ACG8NZ_001636"
     CDS             complement(16373..17242)
                     /gene="accD"
                     /locus_tag="ACG8NZ_001636"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0539309.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17243..18622)
                     /locus_tag="ACG8NZ_001637"
     CDS             complement(17243..18622)
                     /locus_tag="ACG8NZ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0539310.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18630..19049)
                     /gene="fabZ"
                     /locus_tag="ACG8NZ_001638"
     CDS             complement(18630..19049)
                     /gene="fabZ"
                     /locus_tag="ACG8NZ_001638"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0539311.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19074..19550)
                     /gene="accB"
                     /locus_tag="ACG8NZ_001639"
     CDS             complement(19074..19550)
                     /gene="accB"
                     /locus_tag="ACG8NZ_001639"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0539312.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19553..20788)
                     /gene="fabF"
                     /locus_tag="ACG8NZ_001640"
     CDS             complement(19553..20788)
                     /gene="fabF"
                     /locus_tag="ACG8NZ_001640"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0539313.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20807..21544)
                     /gene="fabG"
                     /locus_tag="ACG8NZ_001641"
     CDS             complement(20807..21544)
                     /gene="fabG"
                     /locus_tag="ACG8NZ_001641"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0539314.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21556..22473)
                     /gene="fabD"
                     /locus_tag="ACG8NZ_001642"
     CDS             complement(21556..22473)
                     /gene="fabD"
                     /locus_tag="ACG8NZ_001642"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0539315.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22580..22804)
                     /locus_tag="ACG8NZ_001643"
     CDS             complement(22580..22804)
                     /locus_tag="ACG8NZ_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0539316.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22857..23819)
                     /locus_tag="ACG8NZ_001644"
     CDS             complement(22857..23819)
                     /locus_tag="ACG8NZ_001644"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0539317.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23829..24269)
                     /locus_tag="ACG8NZ_001645"
     CDS             complement(23829..24269)
                     /locus_tag="ACG8NZ_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0539318.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6694 a         5441 c         4250 g         8070 t
ORIGIN      
        1 tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
       61 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
      121 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
      181 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
      241 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
      301 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
      361 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
      421 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
      481 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
      541 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
      601 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
      661 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
      721 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
      781 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
      841 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
      901 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
      961 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
     1021 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
     1081 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
     1141 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
     1201 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
     1261 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
     1321 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
     1381 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
     1441 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
     1501 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
     1561 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
     1621 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
     1681 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
     1741 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
     1801 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
     1861 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
     1921 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     1981 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     2041 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     2101 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     2161 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     2221 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     2281 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     2341 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     2401 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     2461 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     2521 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     2581 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     2641 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     2701 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     2761 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     2821 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     2881 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     2941 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     3001 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     3061 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     3121 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     3181 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     3241 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     3301 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     3361 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     3421 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     3481 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     3541 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     3601 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     3661 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     3721 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     3781 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     3841 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     3901 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     3961 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     4021 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     4081 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     4141 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     4201 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     4261 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     4321 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     4381 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     4441 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     4501 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     4561 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     4621 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     4681 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     4741 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     4801 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     4861 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     4921 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     4981 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     5041 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     5101 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     5161 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     5221 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     5281 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     5341 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     5401 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     5461 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     5521 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     5581 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     5641 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     5701 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     5761 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     5821 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     5881 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     5941 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     6001 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     6061 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     6121 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     6181 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     6241 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     6301 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     6361 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     6421 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     6481 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     6541 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     6601 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     6661 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     6721 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     6781 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     6841 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     6901 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     6961 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     7021 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     7081 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     7141 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     7201 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     7261 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     7321 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     7381 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
     7441 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
     7501 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
     7561 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
     7621 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
     7681 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
     7741 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
     7801 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
     7861 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
     7921 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
     7981 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
     8041 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
     8101 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
     8161 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
     8221 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
     8281 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
     8341 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
     8401 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
     8461 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
     8521 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
     8581 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
     8641 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
     8701 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
     8761 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
     8821 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
     8881 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
     8941 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
     9001 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
     9061 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
     9121 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
     9181 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
     9241 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
     9301 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
     9361 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
     9421 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
     9481 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
     9541 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
     9601 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
     9661 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
     9721 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
     9781 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
     9841 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
     9901 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
     9961 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    10021 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    10081 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    10141 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    10201 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    10261 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    10321 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    10381 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    10441 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    10501 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    10561 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    10621 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    10681 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    10741 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    10801 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    10861 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    10921 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    10981 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    11041 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    11101 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    11161 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    11221 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    11281 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    11341 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    11401 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    11461 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    11521 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    11581 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    11641 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    11701 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    11761 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    11821 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    11881 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    11941 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    12001 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    12061 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    12121 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    12181 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    12241 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    12301 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    12361 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    12421 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    12481 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    12541 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    12601 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    12661 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    12721 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    12781 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    12841 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    12901 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    12961 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    13021 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    13081 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    13141 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    13201 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    13261 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    13321 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    13381 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    13441 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    13501 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    13561 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    13621 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    13681 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    13741 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    13801 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    13861 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    13921 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    13981 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    14041 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    14101 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    14161 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    14221 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    14281 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    14341 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    14401 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    14461 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    14521 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    14581 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    14641 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    14701 aaaaagtcgt catccgtcaa tgagacatct tgggtctcat tataatggcg taatctgaaa
    14761 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    14821 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    14881 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    14941 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    15001 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    15061 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    15121 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    15181 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    15241 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    15301 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    15361 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    15421 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    15481 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    15541 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    15601 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    15661 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    15721 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    15781 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    15841 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    15901 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    15961 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    16021 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    16081 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    16141 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    16201 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    16261 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    16321 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    16381 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    16441 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    16501 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    16561 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    16621 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    16681 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    16741 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    16801 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    16861 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    16921 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    16981 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    17041 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    17101 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    17161 caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt
    17221 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    17281 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    17341 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    17401 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    17461 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    17521 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    17581 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    17641 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    17701 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    17761 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    17821 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    17881 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    17941 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    18001 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    18061 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    18121 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    18181 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    18241 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    18301 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    18361 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    18421 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    18481 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    18541 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    18601 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    18661 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    18721 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    18781 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    18841 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    18901 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    18961 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    19021 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    19081 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    19141 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    19201 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    19261 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    19321 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc aggtatgaca
    19381 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    19441 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    19501 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    19561 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    19621 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    19681 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    19741 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    19801 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    19861 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    19921 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    19981 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    20041 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    20101 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    20161 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    20221 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    20281 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    20341 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    20401 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    20461 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    20521 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    20581 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    20641 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    20701 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    20761 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    20821 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    20881 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    20941 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    21001 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    21061 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    21121 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    21181 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    21241 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    21301 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    21361 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    21421 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    21481 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    21541 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    21601 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    21661 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    21721 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    21781 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    21841 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    21901 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    21961 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    22021 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    22081 cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag cgcctctttt
    22141 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    22201 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    22261 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    22321 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    22381 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    22441 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    22501 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    22561 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    22621 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    22681 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    22741 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    22801 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    22861 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    22921 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    22981 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    23041 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    23101 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    23161 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    23221 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    23281 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    23341 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    23401 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    23461 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    23521 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    23581 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    23641 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    23701 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    23761 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    23821 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    23881 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    23941 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    24001 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    24061 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    24121 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    24181 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    24241 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    24301 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    24361 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    24421 catttataat ttcttttatt agaaaaatca tctat
//
LOCUS       DAZKPO010000043        24337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000043 DAZKPO010000000
VERSION     DAZKPO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.043"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..2477)
                     /locus_tag="ACG8NZ_001646"
     CDS             complement(132..2477)
                     /locus_tag="ACG8NZ_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0539319.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="ACG8NZ_001647"
     CDS             2756..3658
                     /locus_tag="ACG8NZ_001647"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0539320.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="ACG8NZ_001648"
     CDS             3869..5644
                     /locus_tag="ACG8NZ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0539321.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="ACG8NZ_001649"
     CDS             complement(5710..7296)
                     /locus_tag="ACG8NZ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0539322.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="ACG8NZ_001650"
     CDS             7547..7771
                     /locus_tag="ACG8NZ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0539323.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="ACG8NZ_001651"
     CDS             7913..9664
                     /locus_tag="ACG8NZ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0539324.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="ACG8NZ_001652"
     CDS             9664..11448
                     /locus_tag="ACG8NZ_001652"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539325.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /gene="licT"
                     /locus_tag="ACG8NZ_001653"
                     /pseudo
     CDS             11783..12635
                     /gene="licT"
                     /locus_tag="ACG8NZ_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12732..14618
                     /locus_tag="ACG8NZ_001654"
     CDS             12732..14618
                     /locus_tag="ACG8NZ_001654"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0539326.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="ACG8NZ_001655"
     CDS             complement(14659..15561)
                     /locus_tag="ACG8NZ_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0539327.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="ACG8NZ_001656"
     CDS             15791..17194
                     /locus_tag="ACG8NZ_001656"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0539328.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /locus_tag="ACG8NZ_001657"
     CDS             17194..18627
                     /locus_tag="ACG8NZ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0539329.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="ACG8NZ_001658"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="ACG8NZ_001658"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0539330.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="ACG8NZ_001659"
     CDS             19911..21638
                     /locus_tag="ACG8NZ_001659"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0539331.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21654..22004
                     /locus_tag="ACG8NZ_001660"
     CDS             21654..22004
                     /locus_tag="ACG8NZ_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0539332.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="ACG8NZ_001661"
     CDS             complement(22098..22958)
                     /locus_tag="ACG8NZ_001661"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0539333.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="ACG8NZ_001662"
     CDS             23050..23478
                     /locus_tag="ACG8NZ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0539334.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /gene="rpsU"
                     /locus_tag="ACG8NZ_001663"
     CDS             23640..23816
                     /gene="rpsU"
                     /locus_tag="ACG8NZ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0539335.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="ACG8NZ_001664"
     CDS             23843..24289
                     /locus_tag="ACG8NZ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0539336.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5186 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DAZKPO010000044        24274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000044 DAZKPO010000000
VERSION     DAZKPO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.044"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8NZ_001665"
     CDS             complement(55..789)
                     /locus_tag="ACG8NZ_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0539337.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG8NZ_001666"
     CDS             1116..1526
                     /locus_tag="ACG8NZ_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0539338.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG8NZ_001667"
     CDS             1519..2220
                     /locus_tag="ACG8NZ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0539339.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8NZ_001668"
     CDS             2220..3806
                     /locus_tag="ACG8NZ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539340.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8NZ_001669"
     CDS             3825..4046
                     /locus_tag="ACG8NZ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0539341.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8NZ_001670"
     CDS             4043..4804
                     /locus_tag="ACG8NZ_001670"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0539342.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8NZ_001671"
     CDS             5184..5693
                     /locus_tag="ACG8NZ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0539343.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8NZ_001672"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8NZ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0539344.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8NZ_001673"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8NZ_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0539345.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8NZ_001674"
     CDS             7320..8171
                     /locus_tag="ACG8NZ_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0539346.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8NZ_001675"
     CDS             8199..8834
                     /locus_tag="ACG8NZ_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0539347.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8NZ_001676"
     CDS             8854..9627
                     /locus_tag="ACG8NZ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0539348.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8NZ_001677"
     CDS             10035..10832
                     /locus_tag="ACG8NZ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0539349.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8NZ_001678"
     CDS             10810..11223
                     /locus_tag="ACG8NZ_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0539350.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8NZ_001679"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8NZ_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0539351.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8NZ_001680"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8NZ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0539352.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8NZ_001681"
     CDS             16000..16560
                     /locus_tag="ACG8NZ_001681"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0539353.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8NZ_001682"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8NZ_001682"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0539354.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8NZ_001683"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8NZ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0539355.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8NZ_001684"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8NZ_001684"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0539356.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8NZ_001685"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8NZ_001685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0539357.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8NZ_001686"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8NZ_001686"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0539358.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8NZ_001687"
     CDS             21907..22485
                     /locus_tag="ACG8NZ_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0539359.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8NZ_001688"
     CDS             22537..23019
                     /locus_tag="ACG8NZ_001688"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0539360.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8NZ_001689"
     CDS             23136..23510
                     /locus_tag="ACG8NZ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539361.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24274)
                     /locus_tag="ACG8NZ_001690"
     CDS             complement(23885..>24274)
                     /locus_tag="ACG8NZ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0539362.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7907 a         4353 c         5197 g         6817 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttatttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
    24061 actcgattag attggttgag taaatggttc tacgaatgct aggtggaaaa tcataaaaag
    24121 ttaataagtc ttggttttct atgagtgact gcgtcacttt aggatagttt ttcttccatt
    24181 tctcaatcat gccggataag aaggtattcg cttcttcttt tgagttagct tgataaacag
    24241 ccttaaagtc atcacagatt tcttttcggt cttt
//
LOCUS       DAZKPO010000045        23862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000045 DAZKPO010000000
VERSION     DAZKPO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.045"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..331)
                     /locus_tag="ACG8NZ_001691"
     CDS             complement(<1..331)
                     /locus_tag="ACG8NZ_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539363.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(345..602)
                     /locus_tag="ACG8NZ_001692"
     CDS             complement(345..602)
                     /locus_tag="ACG8NZ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539364.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(797..1339)
                     /locus_tag="ACG8NZ_001693"
     CDS             complement(797..1339)
                     /locus_tag="ACG8NZ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0539365.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1360..2451)
                     /locus_tag="ACG8NZ_001694"
     CDS             complement(1360..2451)
                     /locus_tag="ACG8NZ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0539366.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2459..4669)
                     /locus_tag="ACG8NZ_001695"
     CDS             complement(2459..4669)
                     /locus_tag="ACG8NZ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0539367.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4685..7213)
                     /locus_tag="ACG8NZ_001696"
     CDS             complement(4685..7213)
                     /locus_tag="ACG8NZ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0539368.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7265..7675)
                     /locus_tag="ACG8NZ_001697"
     CDS             complement(7265..7675)
                     /locus_tag="ACG8NZ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0539369.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7688..7906)
                     /locus_tag="ACG8NZ_001698"
     CDS             complement(7688..7906)
                     /locus_tag="ACG8NZ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0539370.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7903..8895)
                     /locus_tag="ACG8NZ_001699"
     CDS             complement(7903..8895)
                     /locus_tag="ACG8NZ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0539371.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8974..9885)
                     /locus_tag="ACG8NZ_001700"
     CDS             complement(8974..9885)
                     /locus_tag="ACG8NZ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0539372.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9904..10248)
                     /locus_tag="ACG8NZ_001701"
     CDS             complement(9904..10248)
                     /locus_tag="ACG8NZ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539373.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10268..10546)
                     /locus_tag="ACG8NZ_001702"
     CDS             complement(10268..10546)
                     /locus_tag="ACG8NZ_001702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539374.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10905..11405)
                     /locus_tag="ACG8NZ_001703"
     CDS             complement(10905..11405)
                     /locus_tag="ACG8NZ_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539375.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11406..11951)
                     /locus_tag="ACG8NZ_001704"
     CDS             complement(11406..11951)
                     /locus_tag="ACG8NZ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539376.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11948..13204)
                     /locus_tag="ACG8NZ_001705"
     CDS             complement(11948..13204)
                     /locus_tag="ACG8NZ_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0539377.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13565..13876)
                     /locus_tag="ACG8NZ_001706"
     CDS             complement(13565..13876)
                     /locus_tag="ACG8NZ_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539378.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13899..15374)
                     /locus_tag="ACG8NZ_001707"
     CDS             complement(13899..15374)
                     /locus_tag="ACG8NZ_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0539379.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15823..16275)
                     /locus_tag="ACG8NZ_001708"
     CDS             complement(15823..16275)
                     /locus_tag="ACG8NZ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539380.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16277..16642)
                     /locus_tag="ACG8NZ_001709"
     CDS             complement(16277..16642)
                     /locus_tag="ACG8NZ_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539381.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16741..21024)
                     /locus_tag="ACG8NZ_001710"
     CDS             complement(16741..21024)
                     /locus_tag="ACG8NZ_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0539382.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21171..21410)
                     /locus_tag="ACG8NZ_001711"
     CDS             complement(21171..21410)
                     /locus_tag="ACG8NZ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539383.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21500..21742)
                     /locus_tag="ACG8NZ_001712"
                     /pseudo
     CDS             complement(21500..21742)
                     /locus_tag="ACG8NZ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21855..22106)
                     /locus_tag="ACG8NZ_001713"
     CDS             complement(21855..22106)
                     /locus_tag="ACG8NZ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539384.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22269..23228
                     /locus_tag="ACG8NZ_001714"
     CDS             22269..23228
                     /locus_tag="ACG8NZ_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0539385.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23366..>23862
                     /locus_tag="ACG8NZ_001715"
     CDS             23366..>23862
                     /locus_tag="ACG8NZ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0539386.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         7104 a         5103 c         3609 g         8046 t
ORIGIN      
        1 atgaattcat tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag
       61 acttgtcata caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca
      121 atccattaaa cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa
      181 tttttatacc ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt
      241 gaatttcttg cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg
      301 attcaacgat ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag
      361 ccatgtagag cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt
      421 actctacatt ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca
      481 atgttcggat tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat
      541 acaaacaatc ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc
      601 atctcgtatc catctcctaa aaaatagaat agttcttata gttattcata agacatattt
      661 tttccgtttt tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca
      721 tagtttttct taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga
      781 gtgttcattt tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct
      841 tttgagcttc ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg
      901 tttcttctcc aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa
      961 gtttcccagt tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac
     1021 aacgatctgt catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac
     1081 tccattttct agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt
     1141 gcgctgtctc gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc
     1201 cacttgtttc ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt
     1261 tttcagaaga aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta
     1321 aaattatccc ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag
     1381 actacgaatt tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc
     1441 ggcattctgt tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc
     1501 ggtgtgacca tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc
     1561 tgcttgtaaa tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt
     1621 ataatcttcc cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc
     1681 taattgagat acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc
     1741 aatgatccga tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt
     1801 ttttgttggt aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata
     1861 ggacgttatg atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc
     1921 cgcatagcct ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca
     1981 ttgattaaag ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa
     2041 gttcggaaac ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg
     2101 tgggtaggga caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga
     2161 ttgtgactgt ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa
     2221 ctgatacttt acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg
     2281 tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc
     2341 tagacctgta gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag
     2401 aaaaaaggga agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc
     2461 atcctttctt cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc
     2521 ctggttttgc gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct
     2581 ctgttggctt atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg
     2641 gtttactttc agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg
     2701 ctaccgacga attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc
     2761 catttttcgc ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta
     2821 attgtggcac gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac
     2881 ctttatttaa caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg
     2941 attgtacctg ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg
     3001 aaagaccaga attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat
     3061 gataacgaac atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa
     3121 agggattaac cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact
     3181 tttgaaatcc atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg
     3241 ctaatcctgc acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt
     3301 gtttcatttg agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg
     3361 cagctcctaa ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata
     3421 ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat
     3481 cgtctactat gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt
     3541 gaaacatcaa gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa
     3601 caaaagaagc gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa
     3661 agataaaacg aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg
     3721 gcaccataga accaacgact gctttataaa cagctttagt tggttgaata tagacacgat
     3781 aagccttctt tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag
     3841 ataattcttc acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat
     3901 acaattcttc taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg
     3961 ctttataata aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa
     4021 caccattgac atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc
     4081 cttctacctc cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta
     4141 cgttactacc atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa
     4201 acaccatttt tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat
     4261 acaagacgac aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa
     4321 aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga
     4381 caaaatcaag gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt
     4441 ttagaaaatc tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag
     4501 ataaataaga agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca
     4561 cctgcttttc tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta
     4621 aaaaaaacaa aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc
     4681 atttctattg catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg
     4741 ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac
     4801 gtccataggg atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt
     4861 tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat
     4921 caaaagcaaa gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa
     4981 attgtgtatc taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa
     5041 taatactacg tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg
     5101 ttttcttact actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca
     5161 aacacaaaga ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta
     5221 atttcaaatc ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt
     5281 ctccataaga aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac
     5341 catattctcg aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc
     5401 caacttgttg tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc
     5461 cttttaaaag gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt
     5521 gtttcgcatc tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat
     5581 ttgtagcatc taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat
     5641 gtttctgata atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg
     5701 gatccacaaa taaactttgt acattcataa acgaactata caaaaagagc aagccaacta
     5761 aaaaggtctt ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg
     5821 cacctttgat tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat
     5881 aaattaattc ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt
     5941 gccgaccaat ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt
     6001 cgccaactgc ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc
     6061 ctaataaaca tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa
     6121 tcgtatgaag ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac
     6181 cgtatacaat aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct
     6241 ttaaatgttt tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt
     6301 gaaccaattc cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa
     6361 tacctccctg tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag
     6421 aaagatcctg agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa
     6481 taggcaacaa tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat
     6541 gttccttcgg tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt
     6601 gttcttctac tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct
     6661 ctttttttac taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat
     6721 aaggatcact ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg
     6781 ctaaacttcc tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg
     6841 ttgctttttc tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc
     6901 gttccaacaa tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag
     6961 aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca
     7021 ccaactccac atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta
     7081 tttcctgtgt cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg
     7141 cccagataca atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt
     7201 ttacaagcac caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct
     7261 ttttctaatc ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt
     7321 ttgatacctt gtcccagtta taatacacac aatcaccatt catcatacga atcatataac
     7381 aaaaccaatc ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg
     7441 ctaacacaca taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag
     7501 aaatgaccca accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa
     7561 aagttgctgc attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt
     7621 gtacgcgata aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac
     7681 tatcctccta atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg
     7741 ttggatcatt taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc
     7801 ctacttttga tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat
     7861 agccaccttg cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc
     7921 tttcctatcc aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt
     7981 aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac
     8041 cgttgctttt acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac
     8101 ttgatcaact ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc
     8161 ttttgcattg tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt
     8221 cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt
     8281 ttctgtattc tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt
     8341 tgaaatagaa aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact
     8401 ctctggattc atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc
     8461 aatttctttt aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc
     8521 tggttcttga ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg
     8581 ttcttgttca tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga
     8641 ttcaatttca ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg
     8701 ttggatttca tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc
     8761 caagccccct gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt
     8821 tgaatggatt ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag
     8881 ttgtcgtttt ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca
     8941 acgataagct tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt
     9001 tctctttctt ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga
     9061 aagtgcaaca tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt
     9121 tttgatttca atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa
     9181 attcccttca ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc
     9241 acctgctact tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact
     9301 agtgacaatc ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt
     9361 taccgatact gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc
     9421 tgatttttca acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt
     9481 tggctgttcc gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg
     9541 ttccggagtt gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc
     9601 gatcgaatca gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt
     9661 ttcaggtttc tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt
     9721 tagctctgtc tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt
     9781 aggtgtttct acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa
     9841 tgaaattcct agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat
     9901 tttttatgca attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa
     9961 gaactcccgt ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa
    10021 tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc
    10081 atccgaaaat tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa
    10141 aggatctcta taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac
    10201 atagaacaat gccataccac ataccaccaa aaataataat cgccacataa aaattccccc
    10261 ttttcttcta cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat
    10321 ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt
    10381 cttcatagcc atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct
    10441 cagcttgcat gacataacca aactctgcac catatcgttc tactgttaat aactgttgac
    10501 tacgcaataa tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac
    10561 gtttgtgaaa taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc
    10621 gataatctgg cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta
    10681 ttcgttcagc ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt
    10741 ttttatcacc tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg
    10801 ctctcctaaa aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag
    10861 aaaccgattt ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc
    10921 aaatgacatt ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg
    10981 gatagaatca cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac
    11041 ttacgcttct tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt
    11101 tctactaagc gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca
    11161 tgtttttcaa tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct
    11221 ggatacctta gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac
    11281 gattctaatc gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct
    11341 ggattttcta atactgttgg atcacgtgac caatacgcac caacataaat cataattttt
    11401 ttcatttaga caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg
    11461 gcatagcgac caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt
    11521 tcttctgttg gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata
    11581 agaggttttt cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag
    11641 ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat
    11701 tctagccttc ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta
    11761 cgaacttcta ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct
    11821 tttaccgctt gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt
    11881 tcatttaatg cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta
    11941 tatgcattca tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct
    12001 cgtcatttag ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat
    12061 ctgcttcttg taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac
    12121 gatctaatga gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata
    12181 attttgccca tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa
    12241 gttttgcatt aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa
    12301 taaatgcttc tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat
    12361 aaataccata tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa
    12421 aacgtttctc ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata
    12481 atccttcact tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct
    12541 taaagtccat ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat
    12601 gttcttccgt ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca
    12661 cacgtgtata accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc
    12721 gccaatctgt ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa
    12781 attgcacaag tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc
    12841 cacgaacaga aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca
    12901 gatccattcg taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac
    12961 gcaaataatc aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt
    13021 ccttgattcg ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa
    13081 tgtaatcttt tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat
    13141 ctccacttat attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg
    13201 tcattttctc gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca
    13261 ccgatatatc aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag
    13321 ataatcactt gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc
    13381 cccccatatt atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc
    13441 tttgtgagct gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta
    13501 atgcgcaccg ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata
    13561 ataactacaa aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa
    13621 cgcgataaat tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag
    13681 tcaatcgttc tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa
    13741 cacgttcttt gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat
    13801 aaaacgttct ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat
    13861 aacaactctc tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa
    13921 aaacttgttc cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac
    13981 aactgattat catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag
    14041 acaatcattt cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat
    14101 ggcgcaaaaa actctacagg tacatcaaat cctggaaaag catatccacg accaggttga
    14161 tctttgttgt aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg
    14221 ggagacaatc gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa
    14281 tattctgcat ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt
    14341 aatactaatg gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca
    14401 taaaaagctg cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa
    14461 tttttccctg gttcatattt aggatctttc atggttttca tataggcata ccgttgattc
    14521 atttctttta atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca
    14581 tgtcctttga atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa
    14641 atatctactg tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc
    14701 ccaccaccga ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa
    14761 gttttcatta aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca
    14821 gaatctgaaa ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca
    14881 cgcaaaaata cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca
    14941 ccatctaaag gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt
    15001 aatctcattt tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga
    15061 ttgtcaatta acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct
    15121 tttagtcgcg accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac
    15181 acaatactta ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga
    15241 taaaatactt tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca
    15301 taatagcaaa accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa
    15361 aaatgagctt tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac
    15421 ttttgacaaa aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa
    15481 agatttaatc gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc
    15541 caaatcactt cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca
    15601 cgtaaaaaca tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc
    15661 gttggattgt ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct
    15721 ctcaaagtat ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat
    15781 aaaaagaata ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc
    15841 cttagaatca acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt
    15901 aggattattt gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga
    15961 atattcacga ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa
    16021 tttcacagga tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg
    16081 tcgtgcaatc tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat
    16141 ttctcccgta taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc
    16201 aaagcgtacg gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg
    16261 aaattttaat cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa
    16321 catcttgata gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat
    16381 tcactcgttc tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt
    16441 ctggtaatcg taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt
    16501 cttctcgcga acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct
    16561 ttggtggatt aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca
    16621 tacgcaatgc tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta
    16681 aatatttcta cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga
    16741 ctaatctttg gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac
    16801 taatccaaga accataagta tactacttgt ttgttcacct gtttttggca acattggtac
    16861 aacagtagct ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc
    16921 cacaatttga accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc
    16981 aaacgtttct ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt
    17041 tcctgcaaga ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc
    17101 ggccgcaaag actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta
    17161 gccttgaaca attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc
    17221 aagcacagtt tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt
    17281 cgtcgtaatg gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg
    17341 agaagccaat aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg
    17401 caacgcacgt gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac
    17461 aaacgttaag gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt
    17521 gtccattaga acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac
    17581 agcgtctgta atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg
    17641 tgtagccgtt gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat
    17701 gtctttatgg tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc
    17761 acgaccattt atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc
    17821 tttggcaata aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc
    17881 ggttgcttta tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt
    17941 aatttcaacg gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc
    18001 ttcaaagaca gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc
    18061 gttgatatcc gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac
    18121 gatcgtttga cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt
    18181 gactttgtct gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg
    18241 cgcattcgtt gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa
    18301 gttggtataa gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc
    18361 accatcggct ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac
    18421 agaaaccact aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc
    18481 ttgtttatct gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc
    18541 atcggcaact agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat
    18601 tgcttgattt ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt
    18661 gttccattct tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg
    18721 gatcactttg atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa
    18781 aacgtaattt acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag
    18841 tgtaccgtta gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt
    18901 atcatttgga atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc
    18961 tgtattagca agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg
    19021 ttgttcaatt actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt
    19081 gacttcaagt ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta
    19141 cgtatcaaag acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac
    19201 ggttttcacc actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt
    19261 tggtttgtta tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc
    19321 aatggttcct ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac
    19381 aacaatttca attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa
    19441 aacatatttg ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt
    19501 caaggtaatt tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc
    19561 taaagaagct gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat
    19621 gtttccttgt tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa
    19681 cacaccaatt gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat
    19741 tttattcaat tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc
    19801 attgccgtga atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa
    19861 aaaatcattt ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt
    19921 attaaaagtt gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg
    19981 atcataatca acacggatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat
    20041 aaattgtgcc gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata
    20101 agagatcaag cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt
    20161 agctgtataa acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg
    20221 tccattaatc tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga
    20281 agtaacacca atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt
    20341 tgcacggcga acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc
    20401 tgtttttgga atgttctgct cttttttctt ttgattttca atactagccg tagattcttt
    20461 gtttggtttc gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt
    20521 ttcctcattt tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat
    20581 tcgtttcttt tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc
    20641 ggcagctttt tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt
    20701 ttcttcaact ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt
    20761 ctgatcgaga gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac
    20821 agtggcttca ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc
    20881 gacttgggca atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg
    20941 agcagttccc accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca
    21001 attcattttc gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata
    21061 aaacaaaacg ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg
    21121 gcgctcttat ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca
    21181 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt
    21241 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    21301 tctttccaac catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca
    21361 attgttacag gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga
    21421 tccgattcct cagtattatt gtcattacta gctactggtt tggttgttct actggcttat
    21481 tttgatacaa caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    21541 accactccgc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    21601 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    21661 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    21721 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    21781 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    21841 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    21901 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    21961 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22021 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22081 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22141 ggaatcacaa cttattcgct tcttctttat aaatcgccga ttgtaaaatt aagctagaca
    22201 actaaaaaaa gtcatttgtg ctacactcaa aatagttccg acgaaagaat tataaggagt
    22261 gagtacaaat gacctataac catcttacac caactgaact cgtaatgata gaagcttatt
    22321 tcaatcaatc acaacctgtt tcaaaagtag ccaatctgtt acaacgttct cgacaaacta
    22381 tttataaggt ctatcgtttt cttaaatctg gtggaagcgc aatgagctac tataaacaat
    22441 ataagaaaaa caaacgtaac tgcggaagac atccgattgt tttacctgat gagcaacagg
    22501 aatacattca aaaaaaggtt gttcaagggt gggctcctga tgtccttatt ggtcgagcag
    22561 agtttcctat tgcttgttcg gtacgcacac tgtatcgaat gtttaaagat aaaacctttg
    22621 acactcacga tttaccaatg aagggaaaaa gaaaaccaaa cggtcataaa gaaaaaagag
    22681 gaaaacaagg gtttcgccgg tcaatccgag acagaaagac agactatacc cagtttgatc
    22741 atgaatttgg tcacctggaa ggtgacacta tcgtgggtgg aaaacataaa agcgcggtca
    22801 tcacattagt tgaacggcta tctaaagtca tcattacctt gaaacccgaa ggcagacagg
    22861 ctaaagacat tgaaaatagg ctgaatcaat ggtttcgttg tgtcccaaaa aacttattta
    22921 aatccatcac atttgattgt ggaaaagaat tctcaaattg gaaaaagatt agtaatacca
    22981 acgatatttc aatttatttt gcggatccag gaacaccttc tcaacgagga ttgaatgaac
    23041 attcaaatgg cttattacgt aaagatggtt tgtataaagc tatggatttt aatggtgtat
    23101 ctgaaacctt tatccaatcg gtggcatcga aacgaaatca tattcctaga aaatcattac
    23161 actatcggac accaatggaa gtatttttga gttacgtcac aaaagatgat ttgtctaact
    23221 taatttgaca aacgaaaatt acaaaagtag tgtttcaaga gagtataaaa tattttgtgt
    23281 aaatgaaaaa atccatacaa aaaaggaagt cccttctgta gaataaagtt aacgacaacc
    23341 aattcacaga aaagaggact tccctatgaa tgattttact acagaaattg tgcaaactct
    23401 agtcactaaa ggcgatttaa atgaattatt ccgttcgcac ttagaaaaag cgataaacac
    23461 actcctacgg actgaattaa cggctttttt agattacgaa aaatatgatc gcactggttt
    23521 taattcaggt aattcgagaa acggttctta ctttcgatca atcaaaaccg aatatggtga
    23581 attaacattg gaaataccta gagatcgtaa tggtgagttt aaacaacaaa ctttaccagc
    23641 ctacaaaaga acaaacgata cattggaaac cactattatc catttattcg aaaaaggtgt
    23701 tacgatgtct gaaattgctg atttgatcga aaaaatgtac ggtcatcact atactccaca
    23761 aaccatgtcc aacatgacta aagttctgac tgaagaagta aatgccttta aatccagagc
    23821 cttaaatgat aagtatgtcg ctatttttat ggacgctact ta
//
LOCUS       DAZKPO010000046        23824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000046 DAZKPO010000000
VERSION     DAZKPO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.046"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..960)
                     /locus_tag="ACG8NZ_001716"
     CDS             complement(22..960)
                     /locus_tag="ACG8NZ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0539387.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1451..2668
                     /locus_tag="ACG8NZ_001717"
                     /pseudo
     CDS             1451..2668
                     /locus_tag="ACG8NZ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2802..3041
                     /locus_tag="ACG8NZ_001718"
     CDS             2802..3041
                     /locus_tag="ACG8NZ_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0539388.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            3105..3158
                     /locus_tag="ACG8NZ_001719"
     CDS             3105..3158
                     /locus_tag="ACG8NZ_001719"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0539389.1"
                     /translation="MRNVDKTSTVLKQKSSP"
     gene            complement(3196..4368)
                     /locus_tag="ACG8NZ_001720"
     CDS             complement(3196..4368)
                     /locus_tag="ACG8NZ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0539390.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            4496..4690
                     /locus_tag="ACG8NZ_001721"
     CDS             4496..4690
                     /locus_tag="ACG8NZ_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539391.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            4959..5258
                     /locus_tag="ACG8NZ_001722"
     CDS             4959..5258
                     /locus_tag="ACG8NZ_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539392.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(5355..5789)
                     /locus_tag="ACG8NZ_001723"
     CDS             complement(5355..5789)
                     /locus_tag="ACG8NZ_001723"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539393.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(6021..6731)
                     /locus_tag="ACG8NZ_001724"
     CDS             complement(6021..6731)
                     /locus_tag="ACG8NZ_001724"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0539394.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(6721..7581)
                     /locus_tag="ACG8NZ_001725"
     CDS             complement(6721..7581)
                     /locus_tag="ACG8NZ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0539395.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(7586..8224)
                     /locus_tag="ACG8NZ_001726"
     CDS             complement(7586..8224)
                     /locus_tag="ACG8NZ_001726"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0539396.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(8239..8538)
                     /locus_tag="ACG8NZ_001727"
     CDS             complement(8239..8538)
                     /locus_tag="ACG8NZ_001727"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0539397.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(8581..10065)
                     /locus_tag="ACG8NZ_001728"
     CDS             complement(8581..10065)
                     /locus_tag="ACG8NZ_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0539398.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(10085..10546)
                     /locus_tag="ACG8NZ_001729"
     CDS             complement(10085..10546)
                     /locus_tag="ACG8NZ_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0539399.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(10564..11631)
                     /gene="ulaG"
                     /locus_tag="ACG8NZ_001730"
     CDS             complement(10564..11631)
                     /gene="ulaG"
                     /locus_tag="ACG8NZ_001730"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0539400.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            11849..12619
                     /locus_tag="ACG8NZ_001731"
     CDS             11849..12619
                     /locus_tag="ACG8NZ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0539401.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(12892..13719)
                     /locus_tag="ACG8NZ_001732"
     CDS             complement(12892..13719)
                     /locus_tag="ACG8NZ_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539402.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            14287..15522
                     /locus_tag="ACG8NZ_001733"
     CDS             14287..15522
                     /locus_tag="ACG8NZ_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0539403.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(16521..16781)
                     /locus_tag="ACG8NZ_001734"
     CDS             complement(16521..16781)
                     /locus_tag="ACG8NZ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0539404.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(16808..18109)
                     /locus_tag="ACG8NZ_001735"
     CDS             complement(16808..18109)
                     /locus_tag="ACG8NZ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0539405.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(18401..18859)
                     /locus_tag="ACG8NZ_001736"
     CDS             complement(18401..18859)
                     /locus_tag="ACG8NZ_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0539406.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(18872..20203)
                     /locus_tag="ACG8NZ_001737"
     CDS             complement(18872..20203)
                     /locus_tag="ACG8NZ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0539407.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(20214..21656)
                     /locus_tag="ACG8NZ_001738"
     CDS             complement(20214..21656)
                     /locus_tag="ACG8NZ_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0539408.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(21643..22407)
                     /locus_tag="ACG8NZ_001739"
     CDS             complement(21643..22407)
                     /locus_tag="ACG8NZ_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0539409.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(22511..23386)
                     /locus_tag="ACG8NZ_001740"
     CDS             complement(22511..23386)
                     /locus_tag="ACG8NZ_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0539410.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(23641..23725)
                     /locus_tag="ACG8NZ_001741"
     tRNA            complement(23641..23725)
                     /locus_tag="ACG8NZ_001741"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23689..23691),aa:Leu,seq:caa)
     gene            complement(23743..23814)
                     /locus_tag="ACG8NZ_001742"
     tRNA            complement(23743..23814)
                     /locus_tag="ACG8NZ_001742"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23780..23782),aa:Gln,seq:ttg)
BASE COUNT         7647 a         4777 c         3776 g         7624 t
ORIGIN      
        1 ttttatcaaa taaaacttat tttacttcca aaacctaaat tcacgttgcc aaaaatcaat
       61 ctgcttttgc aattgttttt cgttcgcttt taaagtcgat ttcattaatt ccgttaaatc
      121 aattggagat atttctctaa tcaatttttt aaatttagtc ttagtattct tacttagctt
      181 tccccacata ctttcttcat gcaacaaagt ataaaccata gcttgctcat taattttttc
      241 taaagtagcc cacgcaggtt tcaagatgtg taaatcatta aaacaatcat tccagtaatc
      301 aaccatatct ctttttaatt caacttctac acgccataaa tgttcagaca caacttcaac
      361 atctgcgtta tctttacgtt cttgtttttt attataaatt ctaataaatc tatcactatc
      421 acggacacca aaatattttg tttctggctt gccattacga ccataaaaaa cagttttctt
      481 aactgcttta tcagtcattg catagtaatc gctcaaatca tcttcaaaat caaaagctaa
      541 gtctaatctt gtaaaaccgt catcttccat gtagtcgata atattttgtt ttaaccaaat
      601 catttcttca tgtgtgagtt tattgggatt aaattcaaca cgcatattac gtctatccca
      661 agtatctgct tttactttgt catattcgat ataaactttt tcttgaagtg ccttagcttt
      721 aaactttgtt tgaagtatat cccaaagtcg tatttgtggc tctacactca taaagtcaga
      781 tagcttttta gcattagttt tgttcaaatt tccaacgatt gtcatggcat caaaacttaa
      841 tgcgggttga gattttccca aagtttgacc acttaaccgg ctattactta accggctatt
      901 agagacggcc ggttttttta gtgagatttc tgcagctaat ttttcatcaa ttttcttcat
      961 tttttatcgc tcctaaaata atttttggaa cgaaaaaagg acgagtggaa tcgcaaaata
     1021 aatttcgcct gtatccattc gtccattttg ttttgtattc aaaatcaaaa attgatatac
     1081 taatcatcga atgaataaca agtgtggttt gttattccgg gcgagaacgt tggtagcgtt
     1141 gctcgtcttt ttttatgtct ttttttcgtt aaaactattt taaaactaaa tatatcttaa
     1201 agcaaataaa ttcaaggggc taaaagcgcc ccttaaaaac cccttgcctc tgaaaagttt
     1261 tgtgtttcct ttccgtcggt ttcaaaagct aatttactca aaaaaataga tcaagggtaa
     1321 atgcactcac tgcgttcgcc cttgagctat tttatttcgc aaattttaac gcatttgccg
     1381 acgaaaagat aaaacacata aaaatatacg cagatatatt taaaacgtct tagaacgcaa
     1441 aattttacgt ttaaaagcta tgcaaggact gtatgaagca aaattattga gttatccaag
     1501 aacagataca ccatttatta cagagaacga atttgcttat ttaaaagcga attttggcaa
     1561 atatagcggt tttttaggac ttgatcttga aatggttcaa acagagccta gaaagcgtta
     1621 tgtggacggt agtaaggtac aggaacacca cgccattatc ccaacaaaac aagtacctac
     1681 cgaatctgca ttagcgaaaa tggacgattt acaacgaaaa atttatgctt tagtcgttaa
     1741 aacgaccgtt gccatgtttt tacctgatta cttgtatgaa gaaaccaaga tacaaacgaa
     1801 agtagctgat ttgctgtttc aatcgatcgg aaagacacca aagcaagaag ggtggaaaat
     1861 tcttttcaaa caacaaacca aagaagaaaa agaggacgtt caaacgttac cactcgttat
     1921 cattggcgaa cgtgccgaag ttggtgttaa gagtgttgaa aaagaaacgc aaccgccaaa
     1981 agcttttaca gagggtacat tattaactgc tatgaaaacg gcgaataaaa cggttgatga
     2041 tgaagaagca atcaagattt tacaagaagt tgaggggatt ggaacagaag cgacaagagc
     2101 aagtattatt gaagcgttaa aacaaaaaga atatatccaa gtgattaaga ataagcttgt
     2161 tgtaactgaa aaaggaaaat tattgtgcca ggcagttgaa agtcagcacc ttttaacgag
     2221 tgctgaaatg acggctaaat gggaaacgta tttaaaaaaa atcggtaaaa gagaaggcaa
     2281 tcaagagaac tttattacga atatcaaaaa attcattgtt catttactgg aagctgtacc
     2341 taacgatata gaaaaactaa atttttctga ttaccaggaa cagaaagaaa aagaagcaga
     2401 aaaaagtatt gtaggaaaat gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt
     2461 ttatggttgt tcaaattatc ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa
     2521 aaaactaacc aaaacgaatg taaaagaatt actggaggga aaagaaaccc tggtaaaagg
     2581 aatcaaaaac aaagagaaaa agccctacaa tgccgttgta aaaattgggg aaaagggata
     2641 tattgatttt atatctttct caaaataaac ataaaagccc tttaaagagg gcttttatat
     2701 attaatcaca aatcacttat cacaaatcac ttatcacaaa tcacaagtga tttgtgattg
     2761 ttgatgataa aataagaata agaagaaata gaaagaagtg agtgattgtg ggaaatttag
     2821 gcgcacaaaa agaaaaacga aatgatacac caatcagtgc aaaaaaagat ataatgggag
     2881 ataagacggt tcgtgttcgt gctgacttgc accatatcat aaaaatcgaa acagcaaaga
     2941 atggcggaaa cgtaaaagaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
     3001 tgatagtgca ttatcttaaa attttgtata ataggaattg aagttaaatt agatgctaaa
     3061 aatttgtaat taagaaggag ggattcgtca tgttggtatt ccaaatgcgt aatgtagata
     3121 aaacatctac tgttttgaaa cagaagtcaa gtccataatc ctgtgtaaaa tactatacaa
     3181 tgttttaccg gtctctcatt gatcaaactt aatatatttt cttgtagaac tgagccattc
     3241 gtcatgaagg tccataagga cagctccaat taatcgattg gcagacgttc ggttgggaaa
     3301 aatccgaata atcttttctc ttctgcggac ttcctggttc agtcgttcaa gaaggttggt
     3361 gctttttagc cgattatgac tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc
     3421 attatccaat atttcgcagg cttttgtata tttaggctgg tcgatatatt caccgactaa
     3481 ggcattctta gctgttcgag cgagttcaat atccgtaaac ttaaagatcg cttttactgc
     3541 ttctctaaac ggttttgaat tcttttttgg aatggaactg aagatgtttc ttaaaaaatg
     3601 gacctggcat ctctgccaac ttgcgttggt aaatgactta cgaatcgcag acactaggcc
     3661 tttatgggca tcagaaatga tgagttctgt cccctttagg ccgcgttctt ttaagtattc
     3721 aaagaagatg gaccatgtgt catcactttc ttcattttga atcatgaagc caatgatttc
     3781 acggtcgcca ccttctgtta tcccgatcgc aatatggcag cttttagaaa gcactcgatg
     3841 gtcctcacgg acttttatgt agagaacatc agtcatcaga taaggataat tcgtacctga
     3901 gagtgaacgg ttctgccatt cgttgaccat cgggtctaac tgctcagtca ggctagaaac
     3961 aaaagatttc gatacagatt ttccacatag ctcttcaaca atctttgaaa ctttacgtgt
     4021 cgaaacgccc gaaacataca tctcaagcat tgaagcgagc agtgcctttt catttcgctg
     4081 ataacgctca aacaccgtcg gtgaaaattc accatcacgt gttctaggca cttttaattc
     4141 gagtgtaccc acacgagtcg taaagtctcg ctcataatag ccatttcttt gactctgacg
     4201 actttctgaa cgttcatagt catcggcttg aatatattct gttcgttgat tttccatcaa
     4261 ttggttgaaa acagtggtta aaatattttt agaaacatca tcttttaccg aatgttcaat
     4321 aatactttga acctcttcgt tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc
     4381 tgggtatgtt tttgtggtta aaaacattgt accgtaaaag ggctgttaca tggcctttta
     4441 tcttttacac aattatatgg actttatcaa aaacagcaag aaagaagagg atcaaatgta
     4501 ttatgtagaa gtaaaaacaa aaggcgtcaa aaacaaacaa tatgtgaaag gtatcagtaa
     4561 cgaatatcct ttacttggtt catggaaaga agctgctcct ttttccaaac catgtgccat
     4621 aaaaataaag aacgagctag aaaaagaact gacttgtggt aaagctgttg tagatatcat
     4681 cgaaaagtaa atccaatgta tagaaaggaa gatcacacat gaaaagcgac gtacaaattg
     4741 ctaagattat agaattagaa gaagaagacg cagtgttgac aattgaaaaa aaataaatgt
     4801 ttgtatacca tatgattatt tcaatatgta tcctggaatt ggagatctcg tagagctata
     4861 tcaagatgaa tcaaatatac ttgtcattca tcatgaaata tagagactta taaaattgtt
     4921 tatttttata atgtatcagt atagaaagga aataaaacat ggattatact gttgaatttg
     4981 acagtgaact acttaagcaa tttccacaag aagaacattt catgtataaa gaagcaattt
     5041 cccacaaagt gacagagtat ttggagcata atttttttcg tataaagcca gtgcgtactc
     5101 gttcggcaaa aggaatatac gagatgaaag tcaaaattaa taaaaaaaat tatcgcatag
     5161 catttgcttt aaaaaaaaat catgtgttca ttttttatat tagttcgaat ttgcaaaaaa
     5221 ttgtatttga taaagaggtg agtaaaaaaa taaaatagct gagcattgga ggatagattg
     5281 agtgatacta agtctatcct cttgtttttc taaagaacta agctagaatt ttttcttttg
     5341 tacgttatta tatgttattt gtcattacca aaaagcacct tttttcttct agtatctatg
     5401 acataagaaa taccacccat ttcctttact tgaacaaatc ctactgattc ataaaatttt
     5461 tttgttttct cagtatcatc tgtcaataat tcgatttgga ctggtccata actagtgatc
     5521 ttctccaaag cttcattcaa aagcactttg cctattcctt ggcgctgata cctcggatga
     5581 atgaggatat cttgaataaa tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg
     5641 ataatcttcc cttccccatt acgctttatc agagattcag attgttcaaa tgctctagct
     5701 aaccttgcag tgtcttttgt gtaattcgtc caaccaacgc tcttatataa ttcagaaatc
     5761 tcatcgatca taagggaatc aatattcatt tttttcttcc tttcttatac aattctccct
     5821 ctaataatac agttctttga ctatttaatc tatcacatat aactctcctg taaagattct
     5881 ctcgattcct taaaattcct ctttcctttt agaatgcata ctttttgata aataaaaagg
     5941 ttgtgataaa ttttcttcac aacctctgtt tctctctatc aagtgttatc ttttaatttt
     6001 ctacccttac cctagcatct ctattgcccg tagtatgcat actttccatg tttgcgaaaa
     6061 taatgtttat ctagtaaata ttgcgggatt tcacatatat tggggttgat cagctcgttt
     6121 tctttggcca tcagacaaat ttcatctaag atcaaactgt tttctactgc tttcataggt
     6181 gaatcccccc atgtgaacgg tccatgtcca tagacaagta ctccagggac ttcgtttgga
     6241 tctaattttc tttccttaaa cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct
     6301 tctcttactt cttctttcgt taattggcgt gtacaaggaa ccttaccata aaaagcatca
     6361 gcatgagtcg tgccataagc tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc
     6421 gtggaatgag tatgagtaat agcagttata tcttcaaaag tttgatacaa aacaacatgt
     6481 gtggggagat ctgaagatgg ttttaagcta tcttcttcta ttatatttcc cgacaaatct
     6541 gttaccacca tttgatctgc ttgcatgcat tcatatctga caccgcttgg cttaatgacg
     6601 atgactccta gctcacggtc cacttcactc acattgcccc aagtcaattt gactaatccg
     6661 tatcgcggca atgccagatt tgcttcaagg acacgttgct tcattgctgc aattttttct
     6721 tcacaagaca tcatagccag cctccttcaa tttagaactt aagtagtctt ttgcctgctg
     6781 gatttctctt cgaaagtctt ggcttttttc actccacatt tcaatcagaa atgttccatc
     6841 ataatctaga cgtttcaaat gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa
     6901 aggaacatca cgaaactggc ctgaatattc ttttgttaca gggtaagtat cttttaagtg
     6961 gatagcagtg atataatcga tccctttctc aagttctcga gccggattat tttccggcca
     7021 tgcggacaaa ttgcctaggt ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt
     7081 gatttctaaa aattttgaaa tggagttcat aaatggatca tccatgattt ccacggaaag
     7141 aatgatgcca tatttagaag cttcctttac gctttctttt attccttcga taaagtcttc
     7201 tctggaattc attgatttct cttcataata aacatcgtat ccagctattt ggattacttt
     7261 gacgcttaga ttatgcgcca aatggattgc ttttttcatt agttgtttcg ctgctttttt
     7321 cttttctgga tcagcagaac caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg
     7381 gatacgtaca ccggagctga acatctcttc tctcagctca gctatctttt cttcagacca
     7441 atctaatcgt gctaaccgct catccgtctc atcaatcgac atctcgataa aatcgaagtc
     7501 catttctttt acaagggaaa aacgttcttt ccatgaaata tctttgggta acgctttttc
     7561 ataaatccca atttgagcca tttccttacc cccagatacg aatgatctca tctttgaagt
     7621 tttgtgcggc cttcatcgga tcatttgctg ctgtaatacc acgaccagtg ataaaggtat
     7681 acacatcgat cccttcaaat aagtgtaatg tgtgtggatt taatccacca gttacagaaa
     7741 cattaaagcc tagttcaatc agttttttga tcttcgataa atctttttca ccccaagttt
     7801 caccagataa cagcgcatca cggctttgat ggtagattac ctgccggata ccgatgttat
     7861 gccaatccat ggcttgttca aaagtccaat tgccgtagag ttctacttgc aattcaccta
     7921 tttctttttg tgctgcctcc atagttggaa tcgttgcaca acaaatgact gtcatccagt
     7981 ctgctcctgc ttttgcaaca ttactcgcca ctgttcctcc agcgtctgca catttcgtat
     8041 cagcgactag ttttttatct gggaacatac tgcgaataca gcgaatcgct ttttgtcctt
     8101 cctgtaagca aagaattgtg cccacttcaa taatatctac tacatcacca actttcataa
     8161 catctcctaa tgcatgttct aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc
     8221 tcatcttagt tccttccttt acttgttttt ttggtaccac tttgtttcag caaatttttc
     8281 actgatttct ttgactgaca tcacattttt gataccgata actgtaactc cttttttctc
     8341 tgcctgagta aacatattca aaaaattgac tggcgtaaaa actacatcgt attggccagc
     8401 tgaacttttt ccctctgaaa tggcacaatg atggattttg gtaattggta cgtttagttc
     8461 tttcattgct ttttccacac ttctcatcat catcaagctt gttcctgatc catttgcaca
     8521 cgatactaaa attctcatct taatccctcc attatgagtg ttgtctaatc agattttctt
     8581 ttatactctc tgcttttctg catatgtgtc atagtcttct acaatcaaaa aataaccatc
     8641 aggatttttc cgatattgta actgcggaat agccaataaa ccaatgacaa ctacggcaat
     8701 tccagcataa cctaggactt tcatgattac tgtaaacaat ggccaagctg ttgcccagtc
     8761 gaacatcccg atataaccac cgtatgttga catcccaatc catgtagcga ataatgctga
     8821 accgcctact tgtatgattc ctgaaagaaa cgggaacagg caagcggctt tgaatccacc
     8881 tcggttattg gcataaactg ctataacggc attatcaaag aacaacggaa tgaaaccggc
     8941 tatgacgatc gttggtgatt tcaataaaat cagtaaacca atcatcaaaa actgtcccaa
     9001 agcaccaaac aagaccccaa tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc
     9061 tacgtcaatt cctggaacag ctcctggtaa aatcgtgttt gagattcctt ggaaagattg
     9121 agttagttca tcgacgaatg tccgaacacc taattgcaag atcgccagat ataccgcaaa
     9181 atttaaagaa gtcgtcataa tgtagaataa gaaactctgc ccttcttgca tgaattgcgc
     9241 ttgaatcaaa taatctttgc ctagtacaat cagaatgatc ccaaagaaaa atagcatgag
     9301 taacgaagta gcaaccatat tctcattgaa aatcgacaag aatcctggta atttgatatc
     9361 ttcaattttt ttgtcttgct tgattgatct atttttcttt tcatcccgct ttttcatcca
     9421 ctcagctaat tttgcaaata taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc
     9481 ggcaccttct gtcaattcct gcgtgattcc tactgttaag ttagaagcta ctgtccaata
     9541 acaacctaaa atcagtccca taaaaatcaa tgcttgtatc ctacctagat caggaaagca
     9601 aaacagtaaa atccaaaaag cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa
     9661 aacggaacgc agtttcgtat atttctgaaa acggactaat aagatattta aaacaaatgc
     9721 aatcagtaat aaaatcatca catctccaaa ggtacggcca aatgtctcca ttaatccagc
     9781 atccacagca ttttgtccaa aataaggatc tgtaaccatg gcatccaaat tgaaccgttc
     9841 ctttagtcca accaagattg gccgaaaatt attgaccaaa ccaccagagc caacagtcaa
     9901 aataaagtaa ccgaccgttg cctttaagaa tccagcgatg cattcatgaa gcgggcgttt
     9961 cagcaaaata tagcctaata acacgataaa accaatcagg tatgctggct gcgtcaagat
    10021 atttgttgca aaatacgtcc aaatgcttaa caaaaattcc cccatctttt ttctcctcta
    10081 tcaattaatt atattctgcc atcacaactt ggtaatcatc gatggagttc gtagagatga
    10141 gacgctcaac caatccatct gtcattaata attcggataa tttttggata ttttctagat
    10201 gttcttctgg atcttttgct gcgagtgtaa aaaacaaact agccgttttc tcctcattct
    10261 ccccttcact ttcaaaacgg attggctgtt tcaacttggt aaatgaaata gctgttccaa
    10321 aaacaccatc acttttttct gaagaatgcg gcattgctac ttgaggtgcg atcacaatat
    10381 aaggaccgta tttttctaca caatggatga tctcctccac atacgtttca tcaataattt
    10441 ccttatcgat caatacaaga cagctttcgg cgatagcttc acgccaatcc gtgatttctt
    10501 tatctgggta atgaatcaat tgatggtcaa aaaagtattt cagcaagaaa ctccccttct
    10561 ttcttataaa aacgacggaa acggtgtatc attttcaatc gagaagacat cttcaaatcc
    10621 tcgataaaaa ttaaactcaa gatcatcttt gtttgtcgga tagatgaatt tcccgcctac
    10681 ttgccagata taaggtttaa atccatactt caggcgatcc tttttgaatt tccaaatttc
    10741 agtgatctct tttggatcag ccataaaatt agaccaaata tcgtagtgga caggaatcac
    10801 tacttttgca ttcaatgact ccgccatgcg cagcatatca acagaagtaa ctttgtctgt
    10861 tatccctcgt gggttttcac cataggcccc taaacagaca tcgattttgt gttcattacc
    10921 atgtttggca aataaattgg agtaatgtga atctcctgca tgataaagat tccctcctga
    10981 tgtttcgaat aaatagttta ctgcaatttg atccatctcc tgaggcattt ttccttttaa
    11041 tacctcttct ggatcttcac aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac
    11101 tacattgatg tctttgattt tgactgcatc acctggcttt actgtaatcg ttttttcttc
    11161 aggaattccc catttttgcc acgtgcgaac cacttctttt ggcccaatga atgttgcttc
    11221 aggacaattt tgatgaactg ctgcggctgt atggatatct agatgatcgg aatggatatg
    11281 tgtaacgatt aaagcgtcta cattttttat cgcaaaagga tcgattacga aaggctgagt
    11341 acgtaaatta ggctgcatat tttgaacgcc actcatacgc atcatctgat ggcctttttt
    11401 catcatgcca ttcccatgac tacgttttcc cgtcccacac catagatcac ataaaatatt
    11461 cgttccttca tgacttttca gccaaattcc cgtacagccc agccaccaca tagcaaaagt
    11521 tccaggatcc actacttctg tttcgatttc ttcattcaag tatgtgcccc actcaggaaa
    11581 tgtttctact aaccactttt cttttgttac ttcattgaca gttttactca tttgcttgct
    11641 ccccccatat cgttgttcca acttacaagt aaatattaat gtgtatcagc atccatgaaa
    11701 acccttttat atgaccgttt gttttttata tgattttctt tcagtaatca atgattgttt
    11761 cagtataata aagacagata tgaatgttta aatgaacaac tagtcaaata aatccatgga
    11821 tcaagatgtc cctcaggagg caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg
    11881 tcaaaaagaa attttggaac tgcttcagca gactgaacaa ctatcaactt tcgaattagc
    11941 tgaatccctt gatgtatcta ttagtacgat acgaagagat ttaaatttat tagaagaaaa
    12001 aaacgagatc atacgcaatt acggttattg ttcttataat tacaaaaacc agactgattt
    12061 tgatcaatct ggtcctgaaa ggattaaaca agcgattgcc agagaagcta gtaaatacat
    12121 taacgattat gatacgctgt ttatcaattc tagctcgact gcgttgaaaa cactggattt
    12181 tcttcaatct aaccatttga cagtcattac aaacaacgtg aaaatcaatt actctccgca
    12241 caaagataat tgtagttata ttttaactgg tggagagatg cgctttccaa aagaagcact
    12301 agtgggcgac atcgcattaa atacgatttc ttcagtcaat gcagacattt gtatcattgg
    12361 ctgtagcgga gtcgatgtag tacatggggt cacaacaaag atattaaatg aatcaacgat
    12421 caacgaaatg atggtcaaca agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa
    12481 aatcggactg acatcaaagt tcaagattgc tgatatttca gtctttgatt atttgatcac
    12541 tgataaattt tgttctgaaa aagtattaga ggagatcaga aatacgggga taaaaaccat
    12601 tcaagtagat tcattttaag aaccaggacg taaatatgta aataggatta tcaaaaaaat
    12661 gaataacaaa tttatcgata aatgaaaaac acgctacgct caaattgata aaggaatccc
    12721 aagatgtatc catccaatcg ataccatcat aatctattgc ttcttttata accgttatat
    12781 gttgcctaaa taggaatcaa acaatatcga aaaaaggagg tatgacaaaa gtcacacctc
    12841 cttttttcga ataaacggta ttcaaatcag aacactgatt tcacaaccac tttatttcag
    12901 gtcgttttca atcaccttta ccgcgtaatc atttccgttg aaacgcgcta ttttttttgc
    12961 gtccatatat ctttctaaat aatcatcttc tcctaattgc ttatggcaag aagcaatttc
    13021 ataaagcaag tattccaatg cataagtcga tttatgtttc ttattatact caataccctt
    13081 ctcacataat tggattgctt tatcataatc ctcgatttta cggtaatagg tggcgatatc
    13141 ataatataca atgcagacca tcagattatc aaaatcagtc tcactcaata ttttaagtgt
    13201 acgctccaca taaactttcg caaaatctaa ttctcctttt gacaagtaga tcattgccac
    13261 gtttgcagct gcttgtaagt gatggatatc tgtttcggtc gtttgttcca acacttgatt
    13321 aaaataaaat aatgctgcat ctggtttttt cgcataaaga tgatcaagca gtccttgata
    13381 aaataaaatt tgatttaaaa gatcagaatt acttactttt tcctgatcga tcgtttgtaa
    13441 aattttataa gattcttggt attttgatac agaattcaat gattgaatct catttatttt
    13501 tttcctgatt ttgtcttctt ctgtttctac taatatatcg gagatctcga cattcagctt
    13561 tttgcaaagc gcacttaaga tattgatgcc tggcgacata ttcttgtttt cgatattgct
    13621 gacagttgct tgttcacaaa tgccttctgc taattgtact tgtgttaacc cctttttctt
    13681 tcggtattct tttagtaatg ctccatctac tttaatcaaa acaatttcct tccctttaaa
    13741 aaattatttc tcctttatat catacacaaa aaaaagttct aaacaaccaa aaatgtttag
    13801 aacagaataa aaattttaat tttaaaataa tattcaaatt aaacaactgg cgtatctgct
    13861 ccttgatttc tttttgtgtt atctgaataa ttatcgttta cagttataac acatagtgaa
    13921 aattgtcatg gcgagaaagt ctaagaatat ttttaaacat aactatttct ttcttacgtg
    13981 tgtataatga cgacagcaca cctatcaatt aatataatta ttttattttt ttatgttctc
    14041 atgtttattt ttaatgacac aacttctcgt atttatctgg cacaacatat aaatcgatca
    14101 aaaaattgtt gcgtgttttt ttgaaaattg gagtttcaac ttgattacaa aatttaaatt
    14161 tagaagttgt gaaaacagcg agagacagtt taatattcaa aatatacata ggctactcga
    14221 acgctgtgtt ttttagtata ctagtaagaa acgaagggat agactcattc caataaggag
    14281 gatttcatga agacaattct ttttgctcct gcagtattca atttagctga gacaacacga
    14341 atgattgagg ttgccaaagc tatgaaagac cgttattact gtgtattctt tggcttttct
    14401 aaaacttttg ctcctttgat cacagaaagt ggctttgagt atcatttact agcgccgatc
    14461 cttacgaaaa aacaagaaga acaaatcata aaatttgacc agttgcgtac acttaaaaac
    14521 ccttttacca aagaaatggt tgcctctcgt atcactagtg aacagagtct gattgctaag
    14581 tttgagcctc gactgattgt gacaggttcg aatgtgacaa tttttttatc ggcccgcaat
    14641 aaaaagattc ctttagtata tgttaaaccc tatgctttga gtgaaccttt ttttcaatca
    14701 gagcatgcgc tgatgcccca cgagttacgt ttcttcggcc ctttaagcaa tcccctttgg
    14761 cactggacaa aaaagcgtat agtaaatacc cgatggatcc ccaatgcctt caaacacgct
    14821 gcacaaaaag aaatgcttcc tttaccaaaa acaagtttac aaatgatgga tgctgatcta
    14881 aatctcatca caacgcctaa actatttttg gaagaagaac agctgccgga aaattatcga
    14941 atcgttggtc caatttttgc taagctcagt acttccttgc ctgaagaagt gctaactttt
    15001 atacagcgaa agcaacaaga aagaaaacgt attatctatt tcgcaatggg tagttcaggc
    15061 aatccgcgta tgttacgcaa aattttgttc ttcttacaaa aacaaccgga tctagcggtt
    15121 attgcaccta ttgactatct gcttgatgac attcaatctg actcagaaga tcttttaata
    15181 acagaatatc tgtctagtca tcttttgcat gaattcattg atttttcttt tatccacggc
    15241 ggggaaggga ccgtccaaac tgcctgtgaa tccggcaaac cgtttatcgg tgtggggatg
    15301 caatttgagc aaagctgtaa tattgactat tgtgtaaaat acggaaacgc aatcgccctc
    15361 acaaaaccag tgactgaaag aaaactgcag attgctctgc gcgaagtttc ttcacccaag
    15421 attcgtcagc aggcacgcac gctgcaagcc gtttttccaa taaatggtcc acaacaagca
    15481 attcaagaaa tcgaaacctt cctgagcaac cacccaatat agtttcaagt agaaaaagct
    15541 tttttaatag agactcatca gtaatttcgc tattcgagac ccagacagtg aaaatacaaa
    15601 aaaagtaaat agtggattga aaagttagag ttacttaaat tacggtgtct aaaaaacaca
    15661 aaatcatcct taaaataaaa gatctgacgc tatccaaatg agcataggta gcgtctgcca
    15721 tttattctta gtcaaattct ttgacacctt tctctggttg gatcatctca acgtttcttc
    15781 aaaagaaaga ccataataat taggcaaata cattttctta aaatgtgtca tatagatcta
    15841 gaagcatcgc tttgtagaga tttgtgccgc tagtcccctc aacaattact aaatcaaatg
    15901 taggatttac cactttatct ttgataatca acctccctct tctgttgaga caatgatctc
    15961 gcaatccgat gcatactcct atataaactg ccagcaatcc ccacatattg agtagatcat
    16021 gtcagaatgt tctatgtcgt cagcaacgat acactaaaag tcacgttcac catgactgat
    16081 tgctattccc agcacaatta tttctataca cgtttcatga tttgaataaa cactcacatc
    16141 atacatatta ccattcatac accatactgc agcccctact atatggttgt aagctcccaa
    16201 atctatattt gacttgatca ctttggtagt agatctacta attctaaatc cattgctgat
    16261 aaaatttcca ttttttcctc cttctttctg ccaaaacagt taaaacaata ataatcttta
    16321 tgtaaaaaag ggctgtgaca aaagccttgt cacagtccca tattccgaat aaacggtggt
    16381 caaaagcttg catctgcggt aataagttca accaaacgaa aaataagcca acatttcaca
    16441 aaaatttaag aagcaatttt cgtaaatttc ttcttatttc ttgaagctaa ccacttctgt
    16501 tacaaccact tttagaacat ttaaagcttc gatgtgacaa accatctatg ctgctcgatc
    16561 ataaaattga tagcaaaaat tccacaaagt acatatgtcc cccatgaaaa caaaacattc
    16621 atatgcggta caatgatctt tggaagaatc acgatcaaaa atgatgccag tgcccaaatc
    16681 catcgattga tttgaatatg atatgtagct agcatattca atataaggtg actagccgtt
    16741 aataaaaaga taaaaacaaa aaataaaatc agccaataca ttgtttccca ccttctaaaa
    16801 accaaactta gacagtctgg acttcgtttt ctccacgact ttgttcatct gacagcatct
    16861 ttttatcata agcttttaca aatggataat agataataaa tgctatcaat gtgttgatca
    16921 tgatcaagat aacaacacgc cagtcatttc ctgctgaaag ataaccgccg attggagctg
    16981 gtaatgtcca tgttggcaaa acagtaaatc tattgaccat ttctaatttg actgcaaaat
    17041 aactgataat agtcaaaact aaaggaacta agttgaacgg gatgaacatg taaggattca
    17101 acatcatagg agctccaaaa attatgggct cattgatatt gaagatacca ggaataatag
    17161 aaaaacgacc aacctctttc aagaattttg atttacagaa gaatataaac aaaacagata
    17221 ccacgatcgt tgctcctgca ccaccaattg ttaccgtcca ttgataaaac tgttcaggag
    17281 cgatataggg taattgggtg gcagctgtac catctgccaa tgcctttgca ttctcatcca
    17341 acatgatcag ccacattgga cgaacaacag cccctacaat actcatgcca tggataccaa
    17401 atgaccacaa caaatggatc aaaaacattg gtagtaaaac accaagcaaa ttattcccta
    17461 aaatattggt taaaggctcg aataacgata ataaggcagc gtttacatca aagcccgcga
    17521 cgcttcgaat cagccaagta attgttaaaa tgactgcacc tggaatcaat gcctcaaaac
    17581 tacgggcaac agcaggtggc acttgttctg gcattttgat agtaatattt cgatttttaa
    17641 agaaacgata tacttccact gcaaaaatca tagataagat ccccgcaaac atcccagatg
    17701 ctcccaaaga actcataggt aagacccagc caagaggtgc ctcttcagga gttaaaacgt
    17761 ttacaggaac ttgtaacata aagaacgttc ctaaagataa tataccacct gtcacactat
    17821 ctaatccata tgatttagct agatccgaac ccataacaaa agttgcataa atagccatca
    17881 atcccatcgt catcctaaag ggtaaaacaa tcgttgccga ataaggggca ataaattcat
    17941 tccatccagg aaacggtaaa ttgataaaga ccataaaaaa tgtcccaatc aaaatcaatg
    18001 acaatgttga aacaacccca ttacgaattg ctaataaatg acgctgtgta ccaatttttg
    18061 ccattggtgg gacaagtttc tcttcgaccc atttcatcag cttttccact aaaaccactc
    18121 cttttcaata aaatatgaac cattcattgc atgacaacgc aaagaatgtt cataactaat
    18181 tatataatta agaaatttta tttcaatata ttctcttaaa taatcaaatt tatttttaag
    18241 aatcctgaaa ataagttttc taaatcatag gagcaacaaa aaaattcgat aattaacaaa
    18301 aatcaaaatg attaaatcta gtgattgaaa catatcattt caaaaaaatt ttgtaaattc
    18361 acccataaat atcaccctat ttaacttttt tcatcgtaca ctaaaattca aaacaactat
    18421 ctttaatctt tacttcttgg ttttgtttta ataaatttcc ttcaaaaatg cctggagaaa
    18481 ctacaatgat caccgtatct ttttgtgctt gctccaactt cttcaaatct ataatagcta
    18541 acagtgtccc ttcttttacc tcctggttac tttcaacccg tatctgaaac ggacttcctt
    18601 cgagttcaac tgtatctaaa cccatatgga tcagtatttt tctgccagat tcactttcga
    18661 tcgttatggc atgtcgggta ggaaatatat tatcgattat tcctttaact ggcgcatgga
    18721 tatatccatc atgattcgtt atagcgtatc caactcctat catttttgaa gaaaagacct
    18781 cgtcttttac tgctgttaat ggaataattt gaccactagc tacagctttt agaatgtttc
    18841 ttgttttaaa gaaatgcact ttttatcacc tctactttaa tgctggccaa tattttttgt
    18901 ttactttgat catttcatct agtatttttt ttgcgactct ggcatttggt acggtttgat
    18961 tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc aaagtaagca tctactagta
    19021 acttctctac tgcaacttgt gcctccatca atcctttatg aaaatcactg attggttttc
    19081 ttagtgtaat tggctccaca ccagttgcac caacatatgc cggtatttca acaactgcat
    19141 ctgaccgtag attcggaatc gccccttgat taggaacgat tagcataaag cgacgacgat
    19201 catcattcaa caaggaaata gctatatcga cgatatattg tccatgctcc ccaaaattaa
    19261 agttcaatac ttcttccaca ataccgcggc gtattttatc agccatttct ttggtatttt
    19321 tctctcgtcc atccatgacc atattagcac gtgtatactc tttgtcagta tgctcaacga
    19381 ccatatcagg atataagtaa tactctaagt aattattcgg gatattactt ggaaaatttt
    19441 tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt tttatctcct atattaaaat
    19501 cggcgacttg catttctttt gttattagtt tttcaataat ctctggcata atttctcgtt
    19561 gcaattcttt atcataaatc gatgtatacc agccaaagtg gtttaaacca taatacgtaa
    19621 cgatccaatt cttacgatca taaccgtaat tcactgcaat cgtttcttca attgaaatag
    19681 tcatatcaca ggcattaatc atttttacat tcggatattt acgacgaaca gcttcagaaa
    19741 cgatcgactc tggatttgta taattcaaaa tccatgcttt gggggcatag tcttgtatat
    19801 gatcgacaat ttccaataaa cctttgattg agcgcatccc ataagcaaat cctccgagtc
    19861 cacacgtttc ttgtcctact aatccatgtt taagtggaat cttttcgtcc ttttctcgca
    19921 tctccaatcc tccaacacga atttgtgaga aaacaaaatc gcatcctgta aaagcaagct
    19981 ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc ttttcttttc agcataaatt
    20041 taacaatcaa gtacatatcc atatttcttt cttcatcaat atcatataaa cgtatttcag
    20101 ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac gattcctggt gtatacgtac
    20161 tcccgcctcc agctatagtg ataatctgtt ttttcatcat catcttttcc tttctattct
    20221 tctaataatt tattgaattc tgccataaca taagtcactt ctaagccgat gataatttga
    20281 taattttttt cagttggttt aatgatcccg cttatcccta attgcttgat tcgctgttcc
    20341 tcgaccatgg tgggatccac tacctctacc cgtaggcgtg tattacaact atagacatcc
    20401 aggatattgg cttttcctcc aaacgcttgt agcatttttg ttgccatata ttcataattt
    20461 ttacttgcta accctaactc tgtttcttct aaggttactt cattgccaaa ctctgcctta
    20521 gcttcacgtc ctaatgtctg aatgtttctc ttattgatca aataataaaa agtaacgtaa
    20581 tacaaagcaa aaaagaaaat tccgattgga agaatcaaaa tagcattggt agcgattcga
    20641 aaattgatga gataatcaac aatacatgct ccgaatgtaa aaccaattcg tatattgaat
    20701 aagtaacaga caatccctgc taaaccggca aaaagtgcat ggatcacgta caacatagga
    20761 gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat ttgcaataaa tgaagttcct
    20821 gcaccgcttc ccatgattcc tttgaccatc tctttatttt ctttataagc tgctttatag
    20881 atagctagac atatagctgg tataccaaac atcattacta caaaaaaaac tgataaaaac
    20941 agtccagcct gaggatctcc gttgataaag cgtgtcatct ctcctctgaa aacttccttg
    21001 ctaggagatg tatagcttcc taaatcgtaa taaatatacg tattcaacac atggtgcagt
    21061 ccaaagggga tcagcaaacg attcaaaaac ataaatatcc ccacacctaa tgctcccata
    21121 tcaacgagca gttttgcaaa attatcaatc ccaatttgga tgagcggcca gataaagccc
    21181 aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag tcaatgggaa tttttcgccc
    21241 gcaaaataag aaaaaactac tggtaatttt tgttctttaa agcgattata cgtccatgcc
    21301 gctactaaac cggaaataat tccaccaaaa atccccatat tcacttctgg gtccataatt
    21361 tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag taagtgcagg tattcctttg
    21421 tccttatttt tgacaaaacc gattgcaact cctatagcaa ataagatatc caagtttcca
    21481 aaaacggcat ttccagctgc accaattact gggatgttca gcatatcgtc agctgataaa
    21541 cgcatgatca atcccgctaa tggcatcgct gcaactggaa gtagcatcgc tcttccaagc
    21601 ttctgcacat ttgatttaac attcattttt attcctcctc tattaaatat cctcatatgt
    21661 tactaactca atcaaaggat catttttcac tttttgagca aatgctgaat gaattaaaaa
    21721 ttcgacttgg tgggtacgag gatacacaaa acttgaacca tttaacaatt cctgatccaa
    21781 atacgctgcg tgacacatga cttcgatcgt tttatcttct ctcaatatca tgagtaaact
    21841 tgccaaatat tcttccacac tcgtattgat ttgtcgtttt ttttcatcag tttctcctac
    21901 gacatcgaaa caaggctcaa aataatcgac atgacgaact tcatcttttc gttcaaagtt
    21961 gctccgaacc ggcaaatcat atttttttgc taatgtaaca actacctcct gattttcatc
    22021 caaagtatga gtatgatgat gactgtctaa atgagttggt atgatccccg catgataaat
    22081 tttttgaatc tgagcattcc attcctgata cagttgatct ttgtcaatgt taaatggatg
    22141 ctgataataa gataaggatc tgaactctcc gctttcgata agagtatcga cagttttgag
    22201 gagtggtttg ccacacgtca atgttaaatg gactcctaca cctagcctag gcatttcctt
    22261 tcttagtttc acagcatgct cgaaacccgg catatttgcc atcaatgtag ttgatgtcaa
    22321 aattcctcgc tgataagcat ccataatccc ataattcaca ccatggctat agccaaaatc
    22381 atccgaatta aaaatcactt ttcccatttc attctccttc ttttttctac taaaataggc
    22441 gcaaccaaat aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta
    22501 cgcccttatt ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt
    22561 gaatggcaag ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg
    22621 ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct
    22681 gataaatcaa gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg
    22741 attctttatc ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca
    22801 tttctactat ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat
    22861 gcatagtgat gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc
    22921 gctcgaccac ttctactgct atatcatatt catttggata aaggaatctc atttctggca
    22981 acataattgc tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat
    23041 ctgtcaatgt aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt
    23101 caatcacttc ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga
    23161 atcgtttgat cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa
    23221 atggcttact cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc
    23281 catcgtcatc gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt
    23341 cacctcctct atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct
    23401 ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct
    23461 aattgaatag tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt
    23521 atcaatatct tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat
    23581 tacataaaaa caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta
    23641 taccggcggc cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg
    23701 cgtctgccaa ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg
    23761 aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata
    23821 aaag
//
LOCUS       DAZKPO010000047        23644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000047 DAZKPO010000000
VERSION     DAZKPO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.047"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8NZ_001743"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8NZ_001743"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0539411.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG8NZ_001744"
     CDS             complement(1171..1866)
                     /locus_tag="ACG8NZ_001744"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0539412.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG8NZ_001745"
     CDS             complement(1879..2595)
                     /locus_tag="ACG8NZ_001745"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0539413.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG8NZ_001746"
     CDS             2925..3533
                     /locus_tag="ACG8NZ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0539414.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8NZ_001747"
     CDS             3858..4181
                     /locus_tag="ACG8NZ_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0539415.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8NZ_001748"
     CDS             4363..5733
                     /locus_tag="ACG8NZ_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0539416.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8NZ_001749"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8NZ_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0539417.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG8NZ_001750"
     CDS             complement(7518..9509)
                     /locus_tag="ACG8NZ_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0539418.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG8NZ_001751"
     CDS             complement(9653..9919)
                     /locus_tag="ACG8NZ_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0539419.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8NZ_001752"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8NZ_001752"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0539420.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8NZ_001753"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8NZ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0539421.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG8NZ_001754"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG8NZ_001754"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0539422.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG8NZ_001755"
     CDS             complement(14079..14600)
                     /locus_tag="ACG8NZ_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0539423.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG8NZ_001756"
     CDS             complement(14603..15445)
                     /locus_tag="ACG8NZ_001756"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0539424.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG8NZ_001757"
     CDS             complement(15438..16163)
                     /locus_tag="ACG8NZ_001757"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0539425.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG8NZ_001758"
     CDS             16339..16635
                     /locus_tag="ACG8NZ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539426.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG8NZ_001759"
     CDS             17203..18795
                     /locus_tag="ACG8NZ_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0539427.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG8NZ_001760"
     CDS             18808..19548
                     /locus_tag="ACG8NZ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539428.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG8NZ_001761"
     CDS             19716..21065
                     /locus_tag="ACG8NZ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0539429.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG8NZ_001762"
     CDS             complement(21193..21783)
                     /locus_tag="ACG8NZ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0539430.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8NZ_001763"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8NZ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0539431.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4762 c         4502 g         7610 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aact
//
LOCUS       DAZKPO010000048        23412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000048 DAZKPO010000000
VERSION     DAZKPO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.048"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG8NZ_001764"
     CDS             complement(33..1076)
                     /locus_tag="ACG8NZ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0539432.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG8NZ_001765"
     CDS             complement(1237..1920)
                     /locus_tag="ACG8NZ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0539433.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG8NZ_001766"
     CDS             2115..3044
                     /locus_tag="ACG8NZ_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0539434.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8NZ_001767"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8NZ_001767"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539435.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8NZ_001768"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8NZ_001768"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0539436.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8NZ_001769"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8NZ_001769"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0539437.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG8NZ_001770"
     CDS             5429..6232
                     /locus_tag="ACG8NZ_001770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0539438.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG8NZ_001771"
     CDS             6316..7863
                     /locus_tag="ACG8NZ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0539439.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG8NZ_001772"
     CDS             complement(8160..8357)
                     /locus_tag="ACG8NZ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539440.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG8NZ_001773"
     CDS             complement(8433..8633)
                     /locus_tag="ACG8NZ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0539441.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG8NZ_001774"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG8NZ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG8NZ_001775"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG8NZ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0539442.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="ACG8NZ_001776"
     CDS             complement(11515..12297)
                     /locus_tag="ACG8NZ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539443.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="ACG8NZ_001777"
     CDS             complement(12362..13198)
                     /locus_tag="ACG8NZ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0539444.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="ACG8NZ_001778"
     CDS             complement(13216..14625)
                     /locus_tag="ACG8NZ_001778"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0539445.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14639..16462)
                     /locus_tag="ACG8NZ_001779"
     CDS             complement(14639..16462)
                     /locus_tag="ACG8NZ_001779"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0539446.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(17305..17631)
                     /locus_tag="ACG8NZ_001780"
     CDS             complement(17305..17631)
                     /locus_tag="ACG8NZ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539447.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(17677..18039)
                     /locus_tag="ACG8NZ_001781"
     CDS             complement(17677..18039)
                     /locus_tag="ACG8NZ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0539448.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18076..18290)
                     /locus_tag="ACG8NZ_001782"
                     /pseudo
     CDS             complement(18076..18290)
                     /locus_tag="ACG8NZ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18418..18753
                     /locus_tag="ACG8NZ_001783"
     CDS             18418..18753
                     /locus_tag="ACG8NZ_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0539449.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(18829..20826)
                     /locus_tag="ACG8NZ_001784"
     CDS             complement(18829..20826)
                     /locus_tag="ACG8NZ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539450.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(20924..21199)
                     /locus_tag="ACG8NZ_001785"
     CDS             complement(20924..21199)
                     /locus_tag="ACG8NZ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539451.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            21431..22165
                     /locus_tag="ACG8NZ_001786"
     CDS             21431..22165
                     /locus_tag="ACG8NZ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0539452.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            22204..23346
                     /locus_tag="ACG8NZ_001787"
     CDS             22204..23346
                     /locus_tag="ACG8NZ_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0539453.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         7223 a         4623 c         4094 g         7472 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
    17041 ctgatttatt tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa
    17101 taccaaacat atccataata attttttctt tttcatttct tttatattgt tataacagtg
    17161 aataacataa aaattcccga caatcattgt aatatttttt tgtcacctgt attagtattt
    17221 aatttatagg aaactataaa aactccctag tgaatttaca cctttgggag ttcctgtttg
    17281 gacggtatgc ataatacatt taaatcaatt taacattaac acctcagcat atggtatctc
    17341 cacatatata cggtctttct ttggatagta ctcaatattt atgttgatag cacttccatt
    17401 gtctaatata gctttagccc ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc
    17461 atcacatgat cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca atggtttcga
    17521 tgacttctct aatccagatg tacctgtcat ttttgaaaaa atgataccta taatagccaa
    17581 gacaacgata actataatga taaacttctt gttcatattt actttattca actttttatc
    17641 tcctatctcc aaaataattt gctcttatcc ctcttatcag tacattgaca tccctaaaag
    17701 tacaccaaag attgctccag ctacccctgc tatcatcaaa atcgtaccca tttgttcatc
    17761 tttatctcga tataagtagc ccataataac agcaacagca ccaaatacaa caggaataaa
    17821 tgctaacgct actaccatag aaatccaacc aataatcaaa tacagcttcc ctgtctcttg
    17881 tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt tctttactgc
    17941 gtctgctcca gttgtctcat ctgtttcttg tagtgctccg caaaactggc aatacttact
    18001 ttcatctgtt atttctttgc cacattttct acaatacatg tcttctcctc ctaaaaacat
    18061 tcaattgttt attagttaac tgtttacttt attaaataca tcagaaaaca gctcattcat
    18121 tggtacactt aatatctcag aaagcatctt catttgatag ggtttaggaa tcgttctccc
    18181 tgtttgccac gaacttacac attgctgtgt aacattcatt tttttgctaa ctccgtttga
    18241 gaaagtcctt tttcttttct gtatcggctg agattactta cttttttcat gattctcccc
    18301 tcctgtttta ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaaactt
    18361 caaataccat tacaaataca acaacttgtt gtactatact gagatggggg tgtattaatg
    18421 aaacagaaac aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa
    18481 aaagaattag ctgaaaaaat cttctctaca caacaggcag tagcaagatg ggaaaattct
    18541 gtaacagagc ctaacctcga tagcttaacc gctctttcta gagccctagg aacccctgta
    18601 agccattttc tagacaatgt tgtcgtagat tatgaggaag agtttttggc tctctatcgt
    18661 tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt aaaaaggcaa
    18721 gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc gaactttaac
    18781 ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct agtttaatac
    18841 aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac ttgtaaattg
    18901 ataaagagga actaattcaa ctcctgataa tatcaattct tttattactt catctatccc
    18961 tgctcctttt gctaggtaga ctaaaaaact actacttgct ttcacattgt atagtctctt
    19021 aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc ctctgttttc
    19081 ttttaaagca ttttccacgc tgttattccc aacattacgt agacgttcac aaacagtgtt
    19141 agtaagttct ttctctgtca ttagatatgt tccttgccac gataagccct gtaaaacttc
    19201 atgtgcttca tctaaaatca attgtttttc cttgctaagt tctgatcggg aattttgagg
    19261 aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat aagaaaaata
    19321 ttgatatgta aaatgaaatt tttccagaag tggaaggata tcttcattca atttttgaat
    19381 cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat gattaaagtt
    19441 tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca aaggaacatc
    19501 ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta acttattaca
    19561 tttatctatg tcataagttt taccatagta aaaactcatt tttttagcta attgttcccc
    19621 cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat cagaaagcaa
    19681 gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga aaagtttgtc
    19741 tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg
    19801 tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc gactgtcttt
    19861 ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat tactggaatg
    19921 acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg gattatgatc
    19981 gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca tatcaatggt
    20041 taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca ggcagtcttt
    20101 aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa aatcggtttc
    20161 attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac atgtcatggt
    20221 aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta ttatcactgc
    20281 atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt catcacaccc
    20341 ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt cagtaattgc
    20401 ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc gaatactttt
    20461 ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt ctatgcttaa
    20521 gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt taacagtgtt
    20581 atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat tgcctttatt
    20641 ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa actcatctct
    20701 tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt ctttttttgt
    20761 aactggcttc tctaataatt tttctttatc tacataaatt ggaatattaa tcattgatgt
    20821 ttccatttgt gtttcctcct tataaaattc tgttaaagac taaaacatac agataataaa
    20881 aacaaactct ttattactat atgttttagt cttatttgtt tactcaacct ttataccgtt
    20941 ccatgaaact atcaatatca gattttttaa atctaatgac cgtatcaatt tttataacag
    21001 gtaacccgta ttgagaaaca tattttctaa tcgttcctac tgatacacct agatattcac
    21061 ttgcctgctg atagtccata tactctagag tgactccaga aataatgtct tctagcatag
    21121 gctttagcac tgtaattatc tctttcactt gttgttcaac ttgtatagaa acaatgctat
    21181 caatgatttg tagagacata tttccccctc ctttcgtgat tgcttgaaca aattttctaa
    21241 gaattatctt cgtatttttt gctcaaaata aaagtatttt gttattaacc gattaatcaa
    21301 acaaaaaatg attaccagtt catcgacata cacataatac ctcgataaaa aagaggtgtc
    21361 aatatctttg taaaatttat ttacaacacc cctttttcga ggtatactaa ttttaggagg
    21421 tgtggtcaat atgataaaac gtccaccaat aaattattta gaacgaaaaa agatactcgg
    21481 aacaaaaata aaagctataa gaaagagtaa aaaattaaca caaccagcat ttggtcttat
    21541 gattaacaac ggtcaattaa ttgacaaaaa aactatctac gaatgggaga agggaaccta
    21601 tttaccaata ccagagcgat tatcccgaat tgctgatttg ggaaatatga gtatagagga
    21661 gttagtttgt ggcaatgtag aagaatatat tttgggaatt attctttata gagatagcat
    21721 tgtactagat ggtataacat ttccagataa aaacctgttc cagcatcttc gtcaacagtt
    21781 cccaccagta cactctaatt tagatacatg gctggataga tattccaaat tagaaccaga
    21841 gatgcaagaa tttattgcta acaagacttg taataaagtt aaaaatgaaa aaattagtct
    21901 tttcaatatc ttaaaaatag aagaattatt catcaatgct attgtagaag aatttgacaa
    21961 taacattctt ttcctcacta gcagtataga agaactacta gaaagaatgg tagatgaatg
    22021 gctacctatt caattgaaag acatgtctta tcccgaagaa gctgtgagag aaattactga
    22081 taatataaac aagctagaac aaacgatttc atcaattggg aaaaaataca ctaagaaaaa
    22141 aatgaaggga ggtgatacca tctagtggta agtactcctt gtaaactaag aggagatgat
    22201 ttcatggcaa aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa
    22261 ggacacattg ccaccgatcc acgaactggt gaaaaaataa atactacgag aagaggtttt
    22321 agtagcaaac aagaagctca aaaagattat gacgagtaca gacatcaaat attatacggt
    22381 gttaaaaaga aagcacctga tatgacattt gaagacctgt ataatgaatg gataactcat
    22441 cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag taagatacgc aaataatcaa
    22501 atacttccag catttggtaa gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa
    22561 gtggtagatg aatggcacag taaatatgaa agttacgatt atatgagaaa acagactgca
    22621 caaattttac gctacggggt tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca
    22681 cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa agaggagctt
    22741 aataatttac tggatgcttt taaagatttt ggtaacatga aacaatacgc cttcttcaga
    22801 ttattggcat acacaggtat gagaaagagt gaagtactcg ctttacagtg gaaagacata
    22861 gacacgttta ataaagaact acatgtcaat aaaactcttg ctgtagatga atttggtaaa
    22921 gttataattc aatctccgaa aacaagagct tctagacgag ttatctcttt agatactgag
    22981 actctttcta tcctcaacaa ctggaaatta cagcaaaaag aagagtattt aaaacttgga
    23041 tacaatacta gctctaaaga gcaacatgtt tttacaacag ttaaaaacac tctatacatt
    23101 ccaaacactg ttaatgattg gttaagatat attttgaaaa aatataactt gcctcgtatt
    23161 acacctcatg ggtttagaca tacacatgct agtttattat tagaagcagg agaatctgtc
    23221 aaggtagttc aacaacgatt agggcatgaa aatagtaaag tgacattaga catctatgca
    23281 catattacaa ataatgcacc taagaaaaca ggtcaagact ttgctgatat gatggctcat
    23341 cagtaaaaga gctggtcaaa atctggtcat ttctagtata caggcaaaac aaaaaagcta
    23401 gaacccttat at
//
LOCUS       DAZKPO010000049        23032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000049 DAZKPO010000000
VERSION     DAZKPO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.049"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2563)
                     /locus_tag="ACG8NZ_001788"
     CDS             complement(<1..2563)
                     /locus_tag="ACG8NZ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0539454.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
     gene            complement(2785..3669)
                     /locus_tag="ACG8NZ_001789"
     CDS             complement(2785..3669)
                     /locus_tag="ACG8NZ_001789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0539455.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3764..4201)
                     /locus_tag="ACG8NZ_001790"
     CDS             complement(3764..4201)
                     /locus_tag="ACG8NZ_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539456.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4418..5329)
                     /locus_tag="ACG8NZ_001791"
     CDS             complement(4418..5329)
                     /locus_tag="ACG8NZ_001791"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0539457.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5706..6344)
                     /locus_tag="ACG8NZ_001792"
     CDS             complement(5706..6344)
                     /locus_tag="ACG8NZ_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539458.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6348..8330)
                     /locus_tag="ACG8NZ_001793"
                     /pseudo
     CDS             complement(6348..8330)
                     /locus_tag="ACG8NZ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8510..9175
                     /locus_tag="ACG8NZ_001794"
     CDS             8510..9175
                     /locus_tag="ACG8NZ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539459.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="ACG8NZ_001795"
     CDS             complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="ACG8NZ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0539460.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="ACG8NZ_001796"
     CDS             complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="ACG8NZ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0539461.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="ACG8NZ_001797"
     CDS             complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="ACG8NZ_001797"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0539462.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="ACG8NZ_001798"
     CDS             complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="ACG8NZ_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0539463.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21753..22328)
                     /locus_tag="ACG8NZ_001799"
     CDS             complement(21753..22328)
                     /locus_tag="ACG8NZ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0539464.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22328..22927)
                     /locus_tag="ACG8NZ_001800"
     CDS             complement(22328..22927)
                     /locus_tag="ACG8NZ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0539465.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4531 c         3342 g         8166 t
ORIGIN      
        1 ggatagaaag aacaaattta cctgtatcat caatagttgt ttcggacaaa acagcttgat
       61 tttttttcaa tgaaaccaaa gctcctgctt cgcctgtacc tgtgattgag cgagaagctg
      121 cattgatcaa tgtggtactt aatttagcag gctcaggagg aatcacatcg actgtcgttt
      181 tggcagagtc cagttctgtc acttcttggc cgcctcttac ttttttagca gcaactattt
      241 gcacttgatc ccccgttgtt agtctttctt cgttgggtac ttgaacctca aacaaaccgc
      301 cttgttcgcc ttctccccac ggattatatc ctgtcccttc attttcatct gtttgtcctt
      361 gtgtgagagc agtaatttct acaggtgatt tcgtcgtatc tgcataagta atacgtaaag
      421 tcacttctac ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa
      481 cgacatgccc atagattttt ttatctgcat tcgttggtac tcttaaggat tcaatcatcg
      541 gaagctggtt attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa
      601 attcatttgt taaacctgca ctagggcttg atacaaaatt acctaagtcg gttccattga
      661 tcgataagtc tgtcatatca gaaaaataat ccggatcttt ttcaaagtta tcgaacgtta
      721 ctcctgcctt tcgccataaa gcaatttctg aatgagcgat cttcaaagaa gaatcgccat
      781 ttccttgaaa tacccagcca cctatgccat tagtctcgct tccgatccgt ttgttttgga
      841 aatcgaaata ggtaggagat tctaagtcta cattcaacac atccccactg atgatgccag
      901 tcttatcttc cccagttttc ccgaccatga ctaaataagt tttttcgcca gccataaagt
      961 tcaaatcaat atctccagtt gtatctacgg tagctccgga atctgcgcgt atatcgatat
     1021 ttgatttttc tccagtaacc gtaaaatcag ctgaccctgg actttctaga aaaccagaag
     1081 cgtcataaaa ttggatcgct tggtttcctt gattgagtct gttcacataa gatttgccat
     1141 cccctgcatt tcttaccttc aatgagcctc cattagttac tgttagtttt tgcgtaccgt
     1201 tctcaaaacg aactgcgcta gaacgaccac tttgcttatc aatatcgatg actgatccgg
     1261 ctccttctac gatcaaagaa agtcctttcc ctacaaaacg catcccattg tttccaggtt
     1321 ggtcgccatc tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac
     1381 tagaatctcc ttgcaggcga aatccgccag tagtcgctgt attgattgtg attttcccat
     1441 cattcgacac agcgactctt gatcctgagc ctctcatttg gataattccg cgattgttgt
     1501 cgttggtaat attgatcttt gtatttaccg aaaattcact tcctgagcca ctgactttaa
     1561 atgtgacatt cccgggtagc gtatcgacag cctctccttc atcgctgacg ccatctgccc
     1621 gatcatagtc aagagagacc tttgaaccac tcaagatatg gagtcctggt gctgtttttc
     1681 cactgcccgt ctcatcacct gaatctagaa aaccatagaa ttttcctaga tatggtccat
     1741 aaagctgact accattgatt acatttagct gatagcttct acaaaaataa gctttacccg
     1801 gagcggcatt catcccttct ttcactggtt ggacatccaa tacaccggaa acaccatcta
     1861 atgtgacagt tgcacgaggg gcgtaaatca ggcctaattt agaaccatca tataagtcaa
     1921 gtgtcccggt aaattttatt tctcccgtaa aatgatatct gtttccccaa aaatcggaca
     1981 tataagcaat gataatggca tttccatcga tatttggtat caacccacct ggatgagctg
     2041 ccgttccttt aaaattctga aactcgatcg actgaggtgt ctcattgata tagatgtgat
     2101 gtgtgccgaa attatgagca agtccgccaa aatcgataac gatatctcgc ttgatctcca
     2161 attccctgtc taaagtcaaa ggctgagtta aacgaatctt agctactgat tcgtctgcga
     2221 gaagctgcgt cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacaactc
     2281 taacctgtac ctcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg
     2341 tcttattttc ttcttgcctt gtattagaag taggcgcctc actttgggat tctgtagttt
     2401 cagaagtaat tgattcttct gaaaaagtag tcttgtcaaa actttgattc tctgattgcg
     2461 ttgattcgct tgttttctct acatcggcaa aaacggatgt caccatcaag agagggaaga
     2521 caaacaaaat cgacgcaatc agctttttta catattgatt cattctccac catcctctct
     2581 gacgttttga caattttatt ttaacgtttt aatttatttg taattgtact ttcatattta
     2641 aaatatgtat ttttatggca aatttttctg ttaggtgaaa atttcttttt tgtacaaaaa
     2701 aaggctgtga caaaagtctt gtcacagtcc cttattccga acaaccgatg tcctcctgac
     2761 aactcttcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gtaacacttt
     2821 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
     2881 atcagctaat gacacaacta attgctcaca agtaagatag gctagatttt tcgtagattc
     2941 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
     3001 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
     3061 accgcctact gagttagtta gtcctatcag caagatactg ctctcaattt cttccttctt
     3121 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
     3181 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattgc
     3241 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
     3301 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
     3361 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
     3421 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
     3481 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
     3541 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
     3601 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
     3661 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
     3721 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
     3781 aaaataccca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
     3841 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
     3901 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
     3961 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacgacctt
     4021 gaataataaa tgcaacaatc cccgctataa ttagtgtgcc taataccaaa acggaaaatt
     4081 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
     4141 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
     4201 tttatctact tctacgtgca ttttttaata tcgttttctt tatttaagat tatacagaaa
     4261 aatatctagg gtggctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
     4321 aagtaataat taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt
     4381 attgatttaa atagttcata ttttttattt ataccgctta tttactactt gctgtactag
     4441 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
     4501 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
     4561 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
     4621 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
     4681 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
     4741 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
     4801 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
     4861 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
     4921 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
     4981 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
     5041 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgggaatcga
     5101 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
     5161 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
     5221 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
     5281 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
     5341 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
     5401 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
     5461 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
     5521 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
     5581 aatgttatcg attttgtttt tcttcttccg ctgttgggat attcaaatca gacactttta
     5641 tgttcgattt tccagacatt tttttgttgt ttttattaga aaaagtttgt tttttcattt
     5701 gttttctaaa gcatgatctt cttgtttcct attttggtca gatatggatc atgcgtgacc
     5761 acgataacta acgtaccttg ggaagcctgt tgcgataaga tttcaacgac ttgctcctta
     5821 tttcgtttat ctaatgatcc agttggttca tcggctaaaa tgattttgct attcttcaat
     5881 aagactctcg cacaggcgat tctttgcttc tcaccaccag ataatgtgta gataggcgta
     5941 cttagcttct tgtttatatt tacttttttc aatgcctcat actttgctgt tcgcttatct
     6001 gaaacattta aattcgaaaa cttcaatcca atatctaaat taaaatcgat tgtttcatat
     6061 tcaatcaacc caaaatcttg aaaaagaaaa gagagggcat ttctttttat caagcgcttc
     6121 tctcttccag ttaaatgttt tgtttcttga ttaaataaat aaaattgccc tgaactgaat
     6181 tgatcattca atcctaatat attcaataaa gtacttttcc ctgctccgct ctctccaact
     6241 acagtcacaa aatccccagc atccgcagtg aaacgatcca aatgaagaat cgttttgttt
     6301 cgaatgattt ttttcgcacc ttttatcact atcaacggct ccaattgtta acctccattc
     6361 atcagcaata actgactttt cttttctgta gacaaaataa aaacaactga aagaataaaa
     6421 tcaatgaagc taaagaaaaa aatattaaaa agatatttag gctctctttc tataagaaaa
     6481 aggaagatta cacttgtcgt tccccatttg atgaggttca tcatgaagaa atctttatac
     6541 gtttcaaaga gcgaataacc acttgaacga aagatagcga ttttccgaca attgtagaca
     6601 aaatatatgc ataacatgac atagctcaac aagaagaaag aaaagaaagt gagtaaaaat
     6661 atctgtatca agtaatgaaa tgatctgctt aacactttga cagctatctc ttcagtctga
     6721 gtatacggaa atgataactg caagttatca cgtaactgac tttcttcaat cagctcaaat
     6781 aacctttctt tcgacaaaga gcctatcttg atctttaacg gtggatagat ttctccgctg
     6841 agaatatttc tttcccaata cgtactgttt ttcaatgtca gtatttcaac aaccggataa
     6901 tgttcgatcc aggggataga aggaataaat gtataaatgg gttgattgtt ttttataaat
     6961 agaaatttcg gttctttttc actgatttct tgataatatt ctgttatttg cgccaggtca
     7021 ttttttaaat ctgtatgtct atatcgttca gggatcaata taacagggtg cgtttctttt
     7081 tcggttattt ggatcaacct tccagagaca tcaatcaaag gaaatttctt aagataattc
     7141 agatttattt ttatccctct gccaaactgt tcattttctt cattcaagta atctgataca
     7201 ttgactaata tacttccgtt tttattcaaa gtttcatata attcctgatc ggctgctgat
     7261 gcaaaacgat tggaataaat aaaatccacg tggtttttcc cgatggttac tggataaaat
     7321 acatgatagt cctcaccagc gatttctttt ggtgtttcat gggaaacagc taactgtgaa
     7381 gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg tcaaaagaag agaaaacaac
     7441 aatccaaatg taaaaagaat gtattggata tagttgaacg cttctcttaa cgaagcgatt
     7501 ttttcaacta ataaaagaga aatcaaagag ataccctata ttccgctgaa caagaaaacg
     7561 tacatctgga acaaataaag aaattgctcc ttataataca taaaaataaa accaccaatg
     7621 attcctgcaa ttatgaatgt aataatgatt ttctttttcc ccaataaaca ccacaatact
     7681 tgatgtaatg aaaagccatt attcaacaat aaactgattt ctttattcat caataagaaa
     7741 aaggatgata acaaaaggaa taaaagaatc aatccaatag ttaaatacac tcgataatta
     7801 tatacgtctg tacttgaaat aaccgaatac gaatagctat tttcaaagtc ttcaaaaata
     7861 ttagtggcat aatcagtgtg aaatgcttga ttgaatcgtt ccacaaatag taaaatgaac
     7921 cgttcatagg cttctttttc tctcgtcatc aagaaaaatt caccttcaaa atcttttatt
     7981 ttgatcgatt cttcaatgga cgttacgcga aaggaggtat tagaataggg taccttctcg
     8041 tgagaagatt tgacatcccc ttgataatac aatgtcattt caagctttgg ctgtatcttg
     8101 aagttccaag atgtatctac ttcattggat tcatttactg tttttttcat ataagaaagc
     8161 tcagtttctg atgagacatt ttcaaatacc atgattagct gctcatattc ttcttgccca
     8221 ataaaatgtt gtggcaatcg aatcggagta taagtatctg tttgatggaa aaattcattt
     8281 gtccgataga gtacctgttt atttataata accaaggcaa aaccactcat gataccggtc
     8341 actatgattg tgagtaaaac tgtgaacttt tttttgatta tcaccactct cccttactta
     8401 aatgattctt tgataaaatc ataccgtttt tcatcaaaaa aacgtatcta tcaaatgtat
     8461 aggtcatgta gaataaatct atatactgta gaatcgaaag gagaagaaga tgaacatcgg
     8521 agaaaaaata cgaacttacc gcagaaattg taagcttacg caaaaagaat taggcgaaaa
     8581 aatcggtgtt tccgataaaa caatctctag ttgggaaaat tctcgtacaa tgccagacct
     8641 tgaaatgctg tcacttttac atcagacttt aggtctgccg attgattttc cttctattga
     8701 atcagctacc gaatcaatag aaccaacatt gccggatgaa cctcttgtta tcaagaaatc
     8761 ttattcatca aaacaaccct tctcactcaa aaagacacta tctttctttc tgctcgtctt
     8821 ttgtttggtc tatctttttc ttatcaatta tcaatataaa aatgaaacgg ttgatcgatt
     8881 caatcctttt ttgaaaccac aaatcggcta taccacactc cctgtaggat acgtggaaaa
     8941 ttccatcgaa cattgggtat gtaatgatat ctttgttcat ggtacatggc tgtcttttca
     9001 cgctggagag gtagaaaaag gtaagaatca tgctgtaatc aaacacaaag gaaaacacgt
     9061 catgaacgtt cgcctgctca cttgggaagc cattccctca gattaccaag aaatcctcat
     9121 ccaagactat gacgatgcct taaaaagaag ccctcaggag ccagataggc attaagtagg
     9181 agatgaacgt agagatagaa aaaatcaatg cacatgatct tatcaaaaaa aaacaggggt
     9241 acaaattaat tgaaaaagct ttgagatgaa agttcctaaa taaaaaagtt cagaatgata
     9301 ggtaaaaaaa tgactaacta ttctagtatt aggctatttg tattattctt ctgttatttt
     9361 tatatcaaaa aatccagaaa taactaaata taggtatttc tggcttatag tataatattt
     9421 acgccacagc ccccaatgat tctttttgga atcatgatca aggacggaca tacacaaaaa
     9481 tattttgccc attcgatccg aatagattaa cgactaatct agtaagtaat atagattggt
     9541 acgtagtcaa accagcaaat ggaaaagttg tctattccac cccaagggct ttaaagacag
     9601 catcttccgg cgtttgataa aaaatcaaat taaaggtacc cattaattct gggggtacag
     9661 ttccaatatc agcagcgctt atcataggta caagtacttt tttcgctccg gcatcttttg
     9721 ctacttgtaa acagttagct aaattatcta ccttaatcat agaaccacca attgtaatat
     9781 cccctaaaat aactaaactt ggtaatacag gtttatttaa ggaaactgag cataaagcta
     9841 ttatagttga taacgataat gtttttgtca taccaatgcc attcatatct tgaacatgaa
     9901 cgacaaaatc ttttgtcgtc aaactaatag actgactaac acttccataa ttagctctta
     9961 aatatcggta tgccgaatcc atcgcttctc ttgctccagc atctgaattg atcccagtac
    10021 gttcaaactt tccatttccc gaagtcattt gcgtttccaa tttataggta cctaattgcc
    10081 cagttttgcc ataagatacc gtgtacaaat gccctggctt ccctaaccct tctggaatca
    10141 aagttcctcc accttgttca ggaacagaaa catactcttc tcgctcttct tctaaatcaa
    10201 tgtatgaaaa attgacatca taaaattcca ttccaccaat tttctttaat tgttctttta
    10261 cacgacgacg catttcaaga gcaaatctta gtatttcttc gacatcactt ttttcaaatc
    10321 taccatcagg atacaacagt ttcatcatcc cagatacaat ccgacgaact gcaatcgtat
    10381 cccgctgatt taattgagaa ccaagtttga aatatttatc aaaactatcg ccaaaactaa
    10441 cttttcgaag ctcacgcatg acttcagaaa aataatctgt aatgaagcca tagtcatttg
    10501 taaaaaaatc tggtcgatat ttaggaattt cccaacctgg taaataacaa tgcatccgat
    10561 ccaaaaaggc tgaatccgtc cccataacct ccggaaatgg ctcaaataaa ttcgaagttt
    10621 tcaatattac atcaacgctt tgattaatat ttcctacaaa agccattgat gctgaagcat
    10681 tcttttcttc tttgcctcta gcaaaagatc ctgaagccat gtaatctttc atgatttgaa
    10741 cgccatcatt atccttaaat ttaatcccag caacttcgtc aaaagcaaca cagtcccaca
    10801 tacctacaag tcccattgta cgactagcca tattatagaa aaggtttgct actgttgttt
    10861 gtccacctga aacaaggatt gagtttggtg aaatttcttt atatacatgc gatttacctg
    10921 taccacgtgg tcctagttca cagagattaa aattattttc aattaatgga ataagtcttg
    10981 ctaaatgaag ccattttacc cttttattaa attgtgtagg ttccatacca atggaccgta
    11041 gaagtaagtc aatccattca tttgtagtaa actccttgcg agccatcttg taagcctcaa
    11101 tgtctaaacc aggcatttgg attggatcaa ccttttttgc aataaaaggg ctattccaac
    11161 gatcattctc atcataccaa tattccatcg aaacaataca ccagatcccc ccacctaata
    11221 atcgatcaaa ttttttcgga tactcttcac taatttgaac attttttaat cccaggttag
    11281 agaattctgc ttcataacaa tctttctttt catttaaatg tacagttact ttatcaataa
    11341 ctgtataacc acccaattca cgaatttttg cttttacttt ttctgcttca tcaggacgca
    11401 catagtttcg agcaagaata tttttaacat tttccacacc tttttcaacc aatgtttcat
    11461 ccaatgacga agcatacata ccaagtaaat attccaatac ataaacaggt acattcgcac
    11521 cttctttaat tttctttgtt aaatcttttc tgacaatctt tccgggaaag aattcagata
    11581 actttaaaat ttcctgttgt tccactagaa ttcctccatg tgttcagtca ttataatagc
    11641 aattaaagta tatcatactt aaccagtagg cttgataata caaaagaaaa agtatccaca
    11701 aaaaattgtg gatacctcgt ttttatatat caaaatcaaa tgattctgca aaaggattat
    11761 ctataatgaa ttttattcgc tcggtcaaat catttttttg attttttatc actaaataat
    11821 aagtatcttc aactgtatat cgacgattta caaattcaaa aacaaattta gtaaaccgct
    11881 gatttgcatc ttctgttttt gaatccgcaa catagatttc ttcatttgag atacgttcac
    11941 catattcatc ttcaaaataa agccagaaag atgcggggtc ataaacatca ctaattgcat
    12001 ttttttgata gaattctaac gtggttgata atcctgtaat tctgcggttg ctagtcatta
    12061 attcaattgc tactggttcc tttgcattgc tacctttggc taccgtaatt tctagaacag
    12121 ggactagcat ttcttgaata gatgaccctc catgaacgta gctttgtccc ccgccagctt
    12181 tcttaaaaat acttgaagta atcgggacac aaatgtttct agtgtcatca tttcctaaag
    12241 aatctcccaa agagtaattt ttcgtaccaa ttatttcgta ttcttcttca ctaagaataa
    12301 aacgacgttc tacacgatct ttttcatttc tagatggatt ttcaatctta tctacttctt
    12361 caacgtggct tcttttataa ataaatccat gatcagccgt aactataaaa cgatagatac
    12421 tgccatcatt atgcaagcga ttaataagtc gggaaatttc gcttatcgct aaatcacagg
    12481 cctcaaaaac tcgattttca ttcgagtgat cacctgttct atccacttga tcgtgataaa
    12541 tataaatgac tgatttgcca ttaaatactt ctcgtacctc agccttattc atcttaacca
    12601 catcatcata atttaaagct acagcatttg catctcgttg ctggagaatc gcatttcgat
    12661 tggataaacc attcgttttt ttcccatcaa tcagtacttc tgttccttca ttgtaagtta
    12721 gttgattgtt tggtaataag gcggctttac cgaattcagt aactgaaggt aatacagaaa
    12781 gaaagctagc catgtttgtt gaatacttct tttctctaac taacttacgt tctaaattca
    12841 tccctaactc atatcgaaaa gcatctgaaa taataactac agttttaaca gagcgtttca
    12901 ttacagcttg attataaaat tttcgaatgg atggacgatt cttaaattca aaagtttgat
    12961 tccaaatttg tcctgattga tcaagaaact tcgaataatt tgcttcaact aatcgataca
    13021 attggccata tttttgcttt tcttctatat gatcactatt ccaaataaat ttccgataat
    13081 gcgtatcaac ccaatacatt tccttctcat attgagttag caaattatcc atgttcatcg
    13141 gcaagcacaa tctgttttta tccacaagat aataagcatg tttcaataat tgatactcat
    13201 aaatataatc cgatgcgaaa tgcatttttt cgcgctgctt taaactatca aaaattgttt
    13261 gtcctgcaat tttcgttgta gtgtcagaag cctttaatcg ttctgcataa aacattaata
    13321 tttttgcatg aatctcagaa aatacgtctg cttcaattag atcatcaatt tctatactgt
    13381 ctaataatcc atcaccatca atagcctgat atactttttt agatagctgt tcaaagcttt
    13441 ccatgtaacg tgtatcattc ataaactgat ccatgaaagt aataatgttt gctttcaaat
    13501 gtagtatttc aaatcctttg aacttcttcg gcatctcttg atcaatttga taataaaaat
    13561 agttcaaata aagacacatc gtcagctttt ccaatgttgg ctggctatcc gtaaagccaa
    13621 atttcatttg aataaattcc caaaatttag ctgttaactg atacttatca agttccctta
    13681 aaggattctc cctactttct gtaaagcttt ttagaactac acgcagtaaa tcatttaatc
    13741 gaatactttt cgatttcaac aaaacagcca ttatcattaa ttcaatcgtt tctgatttat
    13801 taattgtcat atgcgtacta aattcaccaa aacttctcat gcgatccttt gcattaaaga
    13861 aattacgata ctgtttaact gtctgatgaa atctaagtgg aatagccaat tcattcatga
    13921 taatcgctaa gcggtctgca ctaaattcct ctgaataatt caatacactc aacaaatgat
    13981 tatcttcatc tttatcatcc caacgaggaa atggcgcata gattaaatag ttagtttcgg
    14041 gatcttcata ttccaataaa cgcttagttt taaattgtgc gtccttttct aatcgataga
    14101 tttttgcatt ttgaatgacc aatgagtcaa tttcttcaag aaaatcctga tttgaatcaa
    14161 accaaaaaat gattttccta catccataat taggaaaacg ttgatttaat ttttcttcaa
    14221 tttgttgcgt atttatatct gtcatcaatc tcacctaatc gttctctttc tatacccctt
    14281 atattgacat aatttggata atttcgcacg caatttaaga ataaaaaaga aagttgttag
    14341 aaaacaactt tcttagataa ctattttttt ggttctttga ccccttttgc aaaaatttgg
    14401 aatttctcgc cggttttagg atcacgctgc aacttatcat aattgacaat tacaccatca
    14461 tctaagtcta attcaattcg ttttaatgct aagtgaccca acttctcgtc atactcacga
    14521 acttctttta attgcttctg aatctttaca atttcttttg tcattgcatt tctttctttt
    14581 gggttatcac ttgttgaaat ttgttgctga cgcaattcga ttcgattgtc atacgctttt
    14641 tgtagttcgt gtaggtattc agtacgtact tttcctaatt gatctggtgt atagcgatgc
    14701 aagtacatta acgctttaaa gccattttgt tttcctgagt ccaattgcca ataaattggt
    14761 cgattggagt atgtttttaa atggtctttg taaaaatctt gtacgaagta acgacgaata
    14821 atttctcgac ttgtcccttt gccacctaaa gtgtcagcga taaactggag attttcttca
    14881 agggtttctt taccaaaagt aatctcaacg aaatcaatga aacgattgac aatgtcaatt
    14941 tggctatcct tgaaatactc atcatcggtg atgagtaaaa cattatcagt atcaggttta
    15001 aatttttgat agcgggaagg atcccattcg ccgccagcga agatgagacc atcttcgtct
    15061 agggagtaac gaccgaagat acaacctatt gaataagaaa taaaatcctt aatttccctc
    15121 gccttatcag gtaaccttgc agagatatat tcatcttgaa cctcttttgt taattctttt
    15181 tcaagagaat aatgattaat gaatatgtcg ttcagttcct cttcattcga ttttagagta
    15241 ttaaattgat taaccaactg cctacttaga cagttaaatg cctctgagag atctttttta
    15301 aacatataac ttaacaaaat gtttcttcta aactcgtatg acgtttcaag agcattatag
    15361 tcttttctag ctatatcgat attttcatcc actttttctc ttattttttt taattttctc
    15421 tcagatgtaa ctttaagaaa aggtagtttt cccattggtc cttcatggta atccaaggtg
    15481 ggagaaataa aagttaaaaa gtgattaact aatttagagt ttacatagcc aaatatttct
    15541 tcaaagtcag ttatatcctt tgagaacaga acagatcctt tagtttcaaa aattgcctga
    15601 ttctgcaatc taaaagatac ttgtccagat gaaagtgtcg accaagtaat tcccttttta
    15661 aaatagtaat taagattttg tggtctagat tttaacttcc cattttcatt aacataattt
    15721 tttatttcat ctccatcatt ctcccagtta accacgtatt cattgtttcc ataccatttt
    15781 ctaaaacgac cacctttagt tgtaggaaac catttttttc tagacttttt agcgtcctct
    15841 aatgttttag catcaaactt tattttgtta aattctactt caaaccaata ccgcaaaaat
    15901 ttattgttat ttccggttgc caacccttgt tttgcatctg caacatcgtt agttaatttt
    15961 ccttgagcaa aatcagtgat tacgttatcg cttgcccagt aagcaatcgg cattcctggg
    16021 atttttttga agttgtcttg attagtacta taaaaataat cgcattcacc atgctgatga
    16081 attgcttcta acgttttaat tttttgaact tccattccac ctttaaaatc tgataatttt
    16141 atatagcttc catcttgttc atttgaattt tttatcgtaa acgtacaaat tggaacggtt
    16201 gcttcttcaa aagctgaata ctccatttga atcaaactag aaatgtttct ttcattaatt
    16261 aaaaatgagc gtaacttttc atagctagaa atgaacatcc aaacaaatgg cgtcataaag
    16321 gaagcatatc cattatcaat cagcatttgg ctattcaatt taataaacac agaaaataga
    16381 tcttttttga catcagaata gttatcattt acatattttt ttaatttctt atccatttta
    16441 ttaagataag gcggattagt gactaccgct acatatttat caactagcat ttcagccacg
    16501 tcaagaatgt gcaagtattt ttcttgtatt tccttcatcc cataaagaat atccatattc
    16561 gtaaattcgt tgaattttga tatatactct cttacttttc ctaagtattc tttagttatt
    16621 gcctgattat ttttatcgac taacttaatg attgatccaa gttctttccc attttcaaat
    16681 tgatcaaaaa ttaactcttt gtattcattc cagcgttcac tatacttatc attgttcaat
    16741 tcactaatac gctcttgata ctcgtctgtc actttaggag aatcaataaa ctctcttaaa
    16801 ttcggcgata gacttttttt caaaaaaaga cgatcgtatt gacgcccttt catcataatt
    16861 gcaaaataag acaattgata cgctctttta tcgatttcaa gaccatataa attgtattca
    16921 acgattgcac gtgcagcatc acgctcagaa tatcctaacg taagataaat ttccatcaat
    16981 acatcgaagg cgtaaactaa aatatgccca gaccccattg cgttatcgat aatttttagt
    17041 tcttctggtt ttaaattact tacagatgtt gcttgttcaa aagcatattt atctggaatt
    17101 aagtatttca acttatttct taattcacta ttgggataat gatctaacca atactttcct
    17161 aaactattgt caaccatata acgaacgacc caatctgtgg taaatagttg agttgctgcc
    17221 ggaatatctt gttttttgac aatcccttta taaatattca ccacttgatt atgtggctca
    17281 ctattatagt actgatacaa ccacccaata atctctacct gtccaccagc ttgaacatag
    17341 aaatcatcct ctggaatact taaaagatca gcgattacac catctttttt aatataagaa
    17401 gctgtaaaca aaagttctgt ataatcttct acttcttcaa atagctcagg taagtaatca
    17461 ttcaattgat tacagatttt tataaaaatc aattgataca gcttgtccat tgcttctgct
    17521 gaattatctg ttttccaagt tctgacttgt tgctgttcat tactagataa ttcttcaaag
    17581 atatcggcat cataaatatc tgtaataata tctggctcaa ttttcccact tatttcacta
    17641 gataatacac gatacgtgtc aggtaaataa tcatttacct ccataaaccg aatggcaatc
    17701 aatcggttaa accacgtata agcaacctct tcgattaact cttgaaaagc ttctgaataa
    17761 gaagtgacag aagcacgttg ttttagttct gcaatcaatt tgtttctttg cgctatttct
    17821 tttccttgaa ttcttattcc tgatttttcg atttcttgta aattgctatc aattttcttc
    17881 acatcttcaa tttttgattc atcaattcct aaacgttttg ctttagtcat tacttcatca
    17941 attaattttc tacgggcatt gaccgcgaaa ttctttatcg ctttcttatc catttttctt
    18001 tcccttctat atgtaacctt tgaaaagaca ggcacctttt tatgcctgtc ttttcactag
    18061 aattgaatcg tgatttctgt attttcttga agttgttcct tcagtttttg attaatttca
    18121 gctgttaact catcaatatc tgctgctgac ttaatagttt taacatgaac gttaagtgtt
    18181 ctcattggta ctgttttctg ttgaatagta ggttttacta tcgctatttc ttcagctttt
    18241 gctgcagagt ctatttcatc tttttcagtt tcacgaatca agtttgctgc ttcaatttgt
    18301 ttacgtgctt gttcttcttg acgtctgcgg ttttcttcat tatcgaaacg ttgattaaag
    18361 atcaaatact gttctgctac gtttaatgat aagaaaacaa gatcatctat agtattcgct
    18421 tgttcaattt ttgctttcag ttcttgaaat tcttggtcaa ctattggtac aaattgatcc
    18481 ttaaaatctt ttccttctaa taatttaaat gttgcttgtt tttcttccgt taatttattt
    18541 aatatttcct gactttccaa ctctaacagt tgattatatt gttcaataaa ttcttgaatc
    18601 aactcattta gtttcatcat atctgaataa ggatcagcct ttttcaaaat ggttttcatc
    18661 tcatgaatca attgatcaat ttcttggcta gaagtttgac gccgactttt ttcgattaat
    18721 ctaattttat cttgtgcatt ctcccagatt tttttctgag tactattttg cttgtggaaa
    18781 tttttaattt tatcatatgt ttcagcccag tcgaataatt catcgcgata ttttttgatt
    18841 gtataaaaaa agtcttcaac atctgacgtt tgaacaatta cttcaagata tttttttatt
    18901 tgctcatatt cataagcact agggtaggta taagtgtttt gtaaatcaaa ataagagcgt
    18961 aaattgtcta attgaccttt taaatatttc ttgaaatttt ccattgtatg ttcattgtta
    19021 ttttcatcaa caatattacg actttgaaac agttctctac cgatttcgtc aaccaatttt
    19081 tttgcttttg gcgaaatcac cgcgcttggt tcaattttta tcttttcact ttctcgtcgt
    19141 ttttgaataa tcattacaat actatttgga tcatccatcc gtgaaatttg tacattgtta
    19201 taaagtaaat caattttacc agacatgaaa agtttagcga tgcaccattc cacatcaagt
    19261 tctgtgaatc catacggttg ttttgtttca aaacgatcac gaattgtttt catcgtaatc
    19321 gttaggtgat ttctcttttg atcattgata aaattgaaaa tttcctgaac tgcttgctca
    19381 tttgatggat cttcaataat ttcatttttg ctttctttca ctaaatataa gatatcttct
    19441 ttttctttag gcgcatcaat ttggtttaat ttatggtata cttcttcagc cattttctga
    19501 agtgctgatt ggaattttgc tttaaagtcg gagcctgtca gttgaatttc tgcaccgtta
    19561 tagaaaaatt ttgcagattt caacagttca tttaattgat cgttgatact tgcacttagt
    19621 tcctgtaatt caccagattt ttccgaacga atcgcgtcaa agttctttac tacactcgaa
    19681 taagtcgttg ttaaataaag attcattcta gcatacattg ctatatcatc tttaaattct
    19741 ttatctgatg gcaattctac gaataataag ttacgggaac tcttaagcgc aagttctgcc
    19801 tcttgacctg ctaaatcaga gtatggtgaa ataaaattaa ctgtcatttc agcatttcca
    19861 gcttgcttgt atggacgttg atctaacact tgtgcataag taaacgtata ccggttttta
    19921 aatttatttc cacctgacat gcctaatttt tcataggcaa aactatttcg gtctagaacg
    19981 ccgtcaaaaa ttcgtttaga tagttctacg atcaaatcac tatctgtaat attttgattt
    20041 ttaatctcac gagtaatttc ctgttcttca tcagtttgga aaatataact atctaagttc
    20101 ttccgcacaa agttttcctt aattaaaagg tccaatgctt cttctactcg ttttttagtt
    20161 tccaggcggt cttcgttgat atttcttatc atcaagctcg taatattttc aacatttggt
    20221 ttgatggctt gatcaacata tttaatcatg aataaaattc gtaagacatt tacgttaaag
    20281 ttatctatat cagttggtct ttgaatatga gaattatttg tagcttgtgt aatcactcgg
    20341 ctaattgctg tatcaatcca acgttctaac gagtaataaa actgatctaa agaaaccaaa
    20401 ctggttgtat cattttcctt atttcgttca gcggcttctt taaacattgc tagtaatgaa
    20461 cgttcaccag acgataagtt tgatccttga tagctgtgat tacgaatctc ggttagaatt
    20521 tgccccaata aattgaattg atagggaata aatggataaa cttctgcgta attttcagca
    20581 tcttggaaga gctttaattc aggactatca acaaagagta atttattttt caattctgtt
    20641 tgaacagttt caaattgtaa ggctaaagaa tcattggcaa actctttttt cttcaaaata
    20701 cgttctttaa ttacttcatc ggcatttgct gaagataaac tcacagttgt ggcaaaacga
    20761 tccttgatct tagaaaaatc attatcagaa ttgttatcaa agtttgttac gtgatcaatc
    20821 gcttcctgac ttgtcactaa tacccaagcc ttaccttcac aaattcgacc caaatcttcc
    20881 acaacggttt gtaactgcaa agtcaactca cgattaccgc cgatatattg tccgacctca
    20941 tcgactagaa aggcaatatg atggtctgct tctttggaat caagatagct tttaactaga
    21001 tttgcaaagt catcaatgct tattaaataa tttcctgatg ctttttctaa aaaattatca
    21061 atagcggctt cactcataaa atcgatttta agtgccgctt ctttgatatc atcttggatc
    21121 caatcaaact catggcgctc ttcttgccat tttgccccat taatctcttc aaaagcagct
    21181 ttgaatgcat caaacttttc ttctttaatt aaccgacgtt caaaatctgc tagatgtgga
    21241 atcgtgctta atccttgcat ttcgttaaat acctttaaaa aaacattcac gattccgttt
    21301 ttgtcttttt taccacctgc atcacttttt gaatcaatat taaacagaat aacatctgta
    21361 tttatttgtg agcaccgttc catcgttgcc aaagtcagtg gatctttgat tttcccatct
    21421 tctttgaagt aatcaatggc tttcttgtca tttatttctc gatttgcgag caagtaagaa
    21481 ataattttta agaaatgaga tttacctgaa ccaaagaacc cctgaatcca aacacccatt
    21541 ttatctgttg gtgtattgat actccgttgg taggcatcaa aaattgtttt aaagtgtttt
    21601 tgtagttctt tcgtaaccac gtattcttct aattcttgtt gaacattttc atcctttgct
    21661 tgaccgacct taataactcc ttgaatattt cggtcaattg gttttgcaaa aatatcttta
    21721 attagcattt aatctcctac cttccatccc aactaaattc taaatgctcg ataataatta
    21781 tcatctttat tggtattaag aattgtcaaa cttaattcat catattcccc tggatacatc
    21841 atcacaactg gtacgcgatc aaatacctga tgcattttat ttaaaataga gtgcgctcta
    21901 attaatggat ataattcgcc aattcctgtc aggaacacaa ctgatttttc tggtgtatgg
    21961 gcatccacat attcaaaaaa tacattttct tcctcaggta aatccatttt caataaacga
    22021 ccaatgcttt ccactaattt ttgtaaacca cgtcgttctt ccatcttaat cgctttttca
    22081 gtgaaaccgc gtttttctag atactctaac ataatcacat agagattata aactttcact
    22141 tcataattat caccaacaga tgttccagat atatcattaa tataatctct tacaattaac
    22201 tcgtctttcg gatcgtagtc aaagatataa tagcctactt cattacctaa accacgattc
    22261 aataaaaaat cttctttttg aattacttca ttcaactttt ccaaatgctc ttgaatcgat
    22321 aatgacatta taaaattcct cccagtgcta gtacaatatc tttacgattc ttgtcaacta
    22381 accattgaat caatcgttta tctaataata tcggagtcat tcgtccatat tctttttccc
    22441 ctctgactaa tcctgtttca attagattcg tttgataagc ggcaataagc cgtttaatcg
    22501 tataatcttt ccattttgaa atatcttctg aacttctccg cttctctgta aaaaaagaac
    22561 ggtaatcaac tggtgttaat tgcaaatttc ctttacgtag attttcagca tatacctcag
    22621 tcataaattc attaaaaaga tcatcaatca taaggatgga aatcaaattc actaactttt
    22681 gattatctat atttaactga ttgaaaagtt gcaatccagc ttcatccagt tgactaactc
    22741 tccttttcac gttctgatag atttcttttt gacggttact gctcactgca aaaaaaatat
    22801 tttcatgttc atttttctct ttaatttcta tatctgtata tccttctagt cgtaattgaa
    22861 tatataactt gaactctgaa aaccaaaagc gtttagaaac tagaccagca gaatattttt
    22921 tattcaatag attgcctcct aatcatctat aattcaataa gtaaacaagt gttttttgct
    22981 catcgttagc attcattttt tagtatctac tatacaatac atatttatta ta
//
LOCUS       DAZKPO010000050        23003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000050 DAZKPO010000000
VERSION     DAZKPO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.050"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..653)
                     /locus_tag="ACG8NZ_001801"
     CDS             complement(<1..653)
                     /locus_tag="ACG8NZ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0539466.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIK"
     gene            complement(1221..2288)
                     /locus_tag="ACG8NZ_001802"
     CDS             complement(1221..2288)
                     /locus_tag="ACG8NZ_001802"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0539467.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2308..2787)
                     /gene="pssE"
                     /locus_tag="ACG8NZ_001803"
     CDS             complement(2308..2787)
                     /gene="pssE"
                     /locus_tag="ACG8NZ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0539468.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2804..3265)
                     /gene="pssD"
                     /locus_tag="ACG8NZ_001804"
     CDS             complement(2804..3265)
                     /gene="pssD"
                     /locus_tag="ACG8NZ_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0539469.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3279..4529)
                     /locus_tag="ACG8NZ_001805"
     CDS             complement(3279..4529)
                     /locus_tag="ACG8NZ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0539470.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4907..6358)
                     /locus_tag="ACG8NZ_001806"
     CDS             complement(4907..6358)
                     /locus_tag="ACG8NZ_001806"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0539471.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6679..7443)
                     /locus_tag="ACG8NZ_001807"
     CDS             complement(6679..7443)
                     /locus_tag="ACG8NZ_001807"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0539472.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7471..8169)
                     /locus_tag="ACG8NZ_001808"
     CDS             complement(7471..8169)
                     /locus_tag="ACG8NZ_001808"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0539473.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8181..8960)
                     /locus_tag="ACG8NZ_001809"
     CDS             complement(8181..8960)
                     /locus_tag="ACG8NZ_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0539474.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8976..9920)
                     /locus_tag="ACG8NZ_001810"
     CDS             complement(8976..9920)
                     /locus_tag="ACG8NZ_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0539475.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9966..10745)
                     /locus_tag="ACG8NZ_001811"
     CDS             complement(9966..10745)
                     /locus_tag="ACG8NZ_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539476.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(11125..13200)
                     /gene="glyS"
                     /locus_tag="ACG8NZ_001812"
     CDS             complement(11125..13200)
                     /gene="glyS"
                     /locus_tag="ACG8NZ_001812"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0539477.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(13202..14119)
                     /gene="glyQ"
                     /locus_tag="ACG8NZ_001813"
     CDS             complement(13202..14119)
                     /gene="glyQ"
                     /locus_tag="ACG8NZ_001813"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0539478.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(14505..15323)
                     /gene="recO"
                     /locus_tag="ACG8NZ_001814"
     CDS             complement(14505..15323)
                     /gene="recO"
                     /locus_tag="ACG8NZ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0539479.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(15466..16365)
                     /gene="era"
                     /locus_tag="ACG8NZ_001815"
     CDS             complement(15466..16365)
                     /gene="era"
                     /locus_tag="ACG8NZ_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0539480.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(16380..16775)
                     /locus_tag="ACG8NZ_001816"
     CDS             complement(16380..16775)
                     /locus_tag="ACG8NZ_001816"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0539481.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(16759..17235)
                     /gene="ybeY"
                     /locus_tag="ACG8NZ_001817"
     CDS             complement(16759..17235)
                     /gene="ybeY"
                     /locus_tag="ACG8NZ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0539482.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(17264..19456)
                     /locus_tag="ACG8NZ_001818"
     CDS             complement(17264..19456)
                     /locus_tag="ACG8NZ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0539483.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(19480..20451)
                     /locus_tag="ACG8NZ_001819"
     CDS             complement(19480..20451)
                     /locus_tag="ACG8NZ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0539484.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            21156..22535
                     /gene="brnQ"
                     /locus_tag="ACG8NZ_001820"
     CDS             21156..22535
                     /gene="brnQ"
                     /locus_tag="ACG8NZ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0539485.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            22817..>23003
                     /locus_tag="ACG8NZ_001821"
     CDS             22817..>23003
                     /locus_tag="ACG8NZ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0539486.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         6504 a         4820 c         3810 g         7869 t
ORIGIN      
        1 tctttaataa aacttgttat ttttgaatgt tgcaaatatg aaatatggaa tatgatatct
       61 tcagatatat aatccctttc tgacggaaac ttgatattgt gctgttgaat tatctcagat
      121 gaatacagcg catgccaaac tgacattggc aatataaaat cgactttata actcggctca
      181 gtgccaatca ttccaagcaa cactttttca attatagcgt tatttgtaaa tatcgtacta
      241 tcatatcttg ctggctttga aataatatta ttatcatcat aatattcata atatccagca
      301 taacatgtgt ctgcattact cctttttata ccatttaaca ttgcttcaat ataattagct
      361 gcaacaaaat catcagaatc aataaaagca acatattttc cttttacatg ttcaagtcct
      421 gtatttcttg ccaatcccaa gccagcatta tttttatgaa taatacgaat ccttgaatcc
      481 tttaatgcat agtcttcaca tattttatta gatgaatcct tagatccatc atccacaatt
      541 ataatatcta ggttcttata tgtttgatta cacactgatt ccaaacaact tcctacatac
      601 ttttcaacat tatatacagg tatgattaca ctaattagat cactcgaatt catccttcta
      661 tcctttcact cacgttttct caattagtca atgacggcat caacgatttt cttaaattct
      721 ctgaaaaaaa tagactctga tcttgatgat gtgaatgaca tactatcttc ttctagaaaa
      781 attatttcct ttaaattatt tattaaatca ataatatgat cctcgtaagt atgctttccc
      841 ttggaataat gaaaatatac ttttgtatta cacaatattg caattttatt tcttaatatg
      901 attatttcac tcattcatta ctgccacata tacacaactt ataaccaccc cctgcaaagt
      961 ttagggtaag ttatcagata atatcttcct cttcatcatc cagaatacaa aatctattta
     1021 tttatagaaa tatcattaat gtttttatat aatgtaccgt gcttgtactc ttgctctatt
     1081 acaagcacta aaacactact attcatttga attcttacac ggataaaaat ttacatcgtc
     1141 tatttcaata ttttttctct ctattttatt cacttaataa gttcattaca taatcgaaac
     1201 attttttatt ttccttgtca tcactccttg ctcccttttt gatacagatt taaaattcta
     1261 tcaaagaaat ttccatcgtt aaatttcgtt attgccaaat ccttcgaaca acgtgaaaat
     1321 tttacgtatt tatctttatc attgattttt agtattgcag atgtcaacgc tgatacgtca
     1381 ccaggttgaa acaaatatcc attttctcca tcacgtactg ctgaagaaat atctcctaca
     1441 tctgtagcaa ttacaggcat tccataagaa atcgcctcta gaatagatat aggcaatccc
     1501 tcgttatagg acggtaaaat catcacattt gatcttctat agcactcctc ctttttttct
     1561 cccacaaccc aacctgtaaa ttcaaaatac gattgcaaat tatattcttc tatcaattct
     1621 ttgagcgata tttcttcatc acctgctcca gcaatctcaa aaataaactt tttttctgga
     1681 tattgttctg caatgttttt aactgcttct accaaatcgt gtacaccttt tcgctttatc
     1741 aaaactccca taaacagaaa tcggcacatt tttgaattcc actttatggt atattctgga
     1801 actacaatag aattttctac aacaacagtt ttacaatttg gctcaatttc tttaattatc
     1861 ttcttccatt tttctccaag tacaatcgtg gaatcagact ctcttaacag caagcgaatc
     1921 tgattttgtt tttttacaga aacactatca taccattttt taaattccga tccatgaaga
     1981 tggatgatta caggaatatt taataatcta aaccacttat tcagcacata tttcctacta
     2041 aaactccctc tgtacgacat gtgcatatga ataatattag gtttactcaa taaagagata
     2101 cctataattt tagcaaatgc atacataaaa tataaagatt taaatattgc atttccaccc
     2161 ttataactcg gtataaaagt catctcgata ttcttttttt cccaattaaa attgagcatt
     2221 tgtgtaatta tacttgtaat tccacctttt accgagctgt cattaccaat cataagtact
     2281 ctttgcataa cgctttactc cttatattta tattttttct attaatacct ctatcgaatt
     2341 aataatcgtt tccgtgttag atacatactt gttaaacatc cgaactcttg tttctttaac
     2401 ggcatcttct aaattttcta attcataaca aggatagata atattcatct catcaaattg
     2461 tctaattaac tgcagttggt gatcatcgac atgctcacca tacttagcca atcgaggaac
     2521 agcaatgacc cttttacctt tttttacggc cccaattata gcacctgtac ctccatgagt
     2581 aatcaccatt tctgcttttt gcatagtttc agtaaactca tccctattta gaaatgcttt
     2641 ataggaataa tttagtggtt tataatcact ataaccactc tgtgcaaatg tactttcgat
     2701 tattactcct ttttcaatta aatcatcaat agcttttaac aatctatcaa attgaaattt
     2761 ttgtgaacct aatgttataa atatcatttt tttctcctat tatttaatat atcccaccca
     2821 aataaatagc attaggataa aattgtagca tagtttccca ctgaacataa aattgatctg
     2881 caaaatgata catcaatttt cctgtctgag tagggctggt tacttttgca aacgattcaa
     2941 tataaatcag tttttttccc atcatttttg ttattaagca gatagggatc atagctaaaa
     3001 cacctgtaca aatcacgaga tccggttttt cctttatata tataataatt gatttgaata
     3061 aattaataag tatagtacac aaaaaaaact tttcttttct atttacttgt ctcatgtagt
     3121 acgttttttc gccttttgac tcagctgaat attctgtctg ttctgttata acaaaactct
     3181 catacttctc catcagtggt tttaacatca ttaactgttc ataatgcccc ccagaggaag
     3241 ccgcaaaaca tatctttttg ttcatattaa cctctctatc agtcacgctg aaaaatatct
     3301 ctagtgtaaa ctttttcttt aatatcatcc aagcaggtat catatcgatt ggcaataata
     3361 gaatcacaca tcgccttaaa ctgctctaaa tcgttgataa ccttacttcc gaaaaaagtc
     3421 tctccatctt tcagtgcagg ctcataaatc accacagtcg cacctttagc ttttacgcgc
     3481 ttcataactc cctggattga actctgacga aaattatcac tgttgctctt catggtcagc
     3541 cggtatatac cgattaaaac tggattttcc ctgttgacat catactgatt atattcagcg
     3601 tagttgtaat agcccgccat ctgaagaaca cgatctgcga tgaaatcctt acgggtacga
     3661 ttagattcaa cgatagcctc aatcaaattt ccaggaacat ctgcatagtt tgccaacaac
     3721 tgcttggtat cttttggcaa gcaataacct ccatatccaa aggatggatt gttgtagtga
     3781 gttccaatac gaggatcgag acaaacacca ttgattatct gctgagtatt cagccccttc
     3841 atttcagcat aagtgtctaa ttcattgaaa tagctgactc gcagtgctaa ataggtattt
     3901 gcaaacagct ttactgcttc ggcttcagtg aatcccataa aaagagtatc cacgttctcc
     3961 ttaattgcac ttttctgcag aagtgttgca aaaacatgag cagcatttaa caagcgctca
     4021 tcagtcatat cagtaccaac aataatacga gatggataca gattatcata taaagccttg
     4081 ctctcgcgca gaaactctgg actaaaaata atgttcttac tccccgttgt ctcacggact
     4141 gattcagtgt agccaacagg aattgtagac ttgatgacca tgatggtatc aggattcacg
     4201 tccatcacaa cctcaataac tgactcaaca gcagaagtat cgaaatgctg agtacgactg
     4261 tcatagttag tcggtgtagc aataacaacg aaatctgcgg atttataagc gctccttgca
     4321 tccaaagtag cagttaagtc aagttctttt tctgtaagat acttttcaat atattcatcc
     4381 tgaattggag acttccgttt attaatcagt tcaatctttt cagggataat gtctaccgcg
     4441 gttacctggt gatgctgcgc caatagtgtg gcgatactca aaccaacata accggtgccg
     4501 gctactgcga tgttgtactt tttcttcaca atatctcctc tttcctctct taagtatgga
     4561 gaatgttact cctacggcta ttcatcatgt caaaataaca cagcttagaa tgttgataaa
     4621 ataattttcc agaatgtgat aaaatctctt tcatttataa accacacatc taattttatc
     4681 tattctccac aagacatgta gagtattctt taataacaag atacgacctt actaaatact
     4741 tcaaaaaatt atcatattta attcataaat ataaatcttc attttaaatg tatgcccaaa
     4801 aatttatctg taatgcgtca tcaaaatcta gattccaaat ctttatcaat ttttatccac
     4861 tcattctacc actttagtaa tattttctat ttataacact ttcgtcctac attgatcccc
     4921 ccctctgaag tacagttttt actgtcttaa ataaaatttt aaaatctaac cccatactcc
     4981 aattttttat atattccgtg tcaagttttg ctacttcttc aaaatcagta atatcacttc
     5041 taccacttac ctgccacatt ccagtaattc ctggcttaat agcaattctt gcacgatgat
     5101 gataatcata ttcttcatac tctgatttaa ggcatggtcg aaatcctata aggctcatct
     5161 ttcctgttag aacgttgaaa aattgaggga attcatctaa agacgtggat ctaataaatt
     5221 gaccgattcc tgtctttttt gttccatctg gaagtatttt atttccgata attcttggat
     5281 caaactccat cttaaacata agattattgc tcatcttatt ttctttcatt aattctactt
     5341 ttctttcttc tgcatccata tacatacttc taaacttata catcttgaac ggtttaccat
     5401 tctgacctat acgttcttgt gcaaagaaaa ttgggcctgg tgaattaata taaataagtg
     5461 gagcaataaa gataaaaata attcccgttg caatacatcc aacaatacct gcaagaatat
     5521 caattgttcg ttttataaat gcctgttgag ttgtcataac attgatactt gttgtaagta
     5581 ctgtatattc tcctatacga ccaatagatt gttttcccaa agcactatct gatacttttg
     5641 caagattcaa atgtacagta aggcccattt cagagcatcg ttcaataaat tctttcatac
     5701 acgaagtact tttattcaaa ataataaaga cttcatctac ccatccctga cataagtaat
     5761 ccgctgcatt tttggaatca gaaacaactg tcactccctc aacatcttca ccaatcatgc
     5821 ttttatctat gataatgaca ccatttattt tatacatttt atagttatgc tctttcacat
     5881 tacgaataac tacttcagca atactactag atgtaactat aataagtgat ttgttcccct
     5941 catttttcat tttatgtttc aagtaatgtt tccataagag gcgagttata tatgtaatta
     6001 caacgtataa acctcccatc aagaaaagaa cgactctaga ataatcattt ccaccctgaa
     6061 cactaatgag atacaaactt cctaacagca ccaacataat cgcttgcttt acagaagaag
     6121 caaattctat atagtaccct ctttttaata cacctctata tgcttcaaac atgaaaataa
     6181 ctaaaagatc tgccagttca atgaagactg ccatgttttg atacattgga atcacataag
     6241 gattccaatc tacctggcgt aagacatacg atagataaaa agcaagctgg atacacaata
     6301 aatctaaaat aataaaatca atatgtttaa cccatcccct tgaacttttc ttgtacattc
     6361 ctttttactc ccctcttttt aataaaataa attgtattac ttgttttctc tcctaaatat
     6421 ctttctgaag gattattgac tcgacaaatt ggcttgtgga tattcttata tatagaacat
     6481 ccgtaagcca acttaaaact ccttaacaaa atttttttac tcatattgta gatcaaaaca
     6541 agtatccaag aaatcatatt ttatttcttt tttctgctta ctcaacaaca gctaaaagaa
     6601 aaatcatcat aaacaaattc aactaagcca acgatcccct tgtgaaatca agttttttct
     6661 tgatttacga ataaagagct aaaataaacc aaacttcttc ttttttacct cttcatagac
     6721 tggataactg atttcatccc cgttgatcag atcctttgcg acttgtttca tctgtactac
     6781 tttttcctta ccaaagtcct ttgcaatctg ctcatagcac tcttttaagt aaaaggtgcg
     6841 cttagagacc ccatgggcat cagaagcagc catctgcacg agattatgtt tgaccatcgt
     6901 tttggctgtt ttctggatgt ctttgccaaa taccccaaca taacttggcg cagtgagttg
     6961 ggccaaacat cccatatcta ggaacggaag caaatgattt gggtcttcgc gaaaatgcac
     7021 gttgcgttct ggatggacga ttactggtgt ctttcctagt tctaacaact caaaaaaaag
     7081 ccgttctgca tataacggca cttccagtgt cggaaattcg atcagaagat atgtatcatc
     7141 taaatctgta aataatattt catctcggtg gatgtcttcg atgagcgttc cggtaatacg
     7201 tacttcttgt ccttccaata acgtcaaagg caactgtctt ttctctaatt cggcttgtaa
     7261 cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat tattgtggtg
     7321 tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca ttgcaatact
     7381 tgcttctaag ttttctgccc cgtcatcgat tcctggtagg atatggcaat gtagatcgat
     7441 catttctctt gtctcctctc ttcttattcc ttaatttccg tagtaataac cagcatcttt
     7501 gctatgttct gctccgttgt acacaacacc taagactcgt gcttgtacca tctccagcag
     7561 ttcttttgct ttagtcaagg attctttacg tgacatattt tcacgtacga ccaataaagt
     7621 gccatccgtt tttaaagaca tgatctgtgc atctgttacg gctactactg gtggcatatc
     7681 gaatatcacc acgtcaaata actgtcttgc ttcggatagg atctgatcca ttcttggcga
     7741 gcttaacaat tctgatgggt tgggtggctt aggtccacta ggcaaaatcg acaagttctc
     7801 gactggcgtt cgttggatca catctgctac acttcctgat gaactaagtg ctgtacttaa
     7861 tccactggca ttgttcagct ggaatgtctt atagacgacg ggtttacgta aatctgcatc
     7921 gactagtaga actcgttgac ctgatttagc aaaaacgact gcaatgtttg ctgcagtggt
     7981 ggatttccct tctccaggac ctgatgaagt cacgacaatc gttttgattt gctgaccagc
     8041 tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga ttggtgaaga
     8101 tgggtttgtt aatgtgatca aactgactgc atgagtttgt gctgcttttt gtttctgtgt
     8161 tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg ggctgtttta
     8221 tcgtttgtag cttttggtcg tgcaggagct gttccttttt tacggatacc agcgttcaat
     8281 tctttcgctg acatttctgg aactgttcct aagattggga atcctaatgt atctgtcaca
     8341 tatttttcat ctttcactgt ccgatcgagt aattccaata agaatgccag acctacacct
     8401 accatcattc ctaacattaa gccagtcatt aaattcaatt tattatttgg cgaaacaggt
     8461 gtcgtattgg caactgcacc tgaaatgatc gagattttat caacattcat cacatctttt
     8521 gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct gttgagcagc cactgcattc
     8581 gtacttattg cctgaatcga gaacatttgt gaattttctg cctgattaac tgagatggca
     8641 tctttgattt gttcagcagt cattttcaca ttgtcttctg tttctaaacg atctttgact
     8701 tcattgagca ctaaatcact gacgatcata tctttatacg tattgatcat ttgcaggttc
     8761 gtattgacat cattgacatt tgcagtgtct gattgtggca gtgtcacgat caattgtgct
     8821 tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat ggccaagcca
     8881 gcaaacattg agatgatgat tattaccaag tgtttgcgta atgtttcaaa aatttctttc
     8941 aagctgattg tctcttccat tttttctcca ctttcctatt tattctctag attcatttga
     9001 tcttttaatt cttttttcac acgatcaagt tcttgatcac tgactttttg ataggagata
     9061 ttatcttgca tgaagccttc tccttgcagc tgatcttgct taatcgtacc aaatgcctcc
     9121 cgataatcta aagcaatttt tttcatttca tcaaagttca tgtctgtttt catattcgac
     9181 tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttagactcaa tacttttttc
     9241 gctacgcctt ctacgatctt tctttggcgc ccttgtctac cgtaatcacc atttgggtct
     9301 tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtaat tttaccgatt
     9361 ggaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat accaccaact
     9421 gcatcaacga gttcctttag tcctttcata ttgatagaga catagtgatc gattgggatg
     9481 tctacatatt tttgtacggt atccatcgcc atggcagctc cgccgaatgc gtaagcgtgg
     9541 ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatctcg aggaatgctg
     9601 acgatcgttg tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt atctgatctt
     9661 ccttgatcga cccgacctaa atctccagta tcaatcccca tcaatagaac agagaaagat
     9721 tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc cactgattcg
     9781 taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac tcctacaatt
     9841 gctaatacta atacgataag aactgctaaa ataatcaaaa atattttttt ccctcttgat
     9901 tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa tcaacgtttt
     9961 aactgtcaat agcttatatt gctatagccc atttgctgag caacagtttg tgcttttgtt
    10021 gcgtatgcgg ctcttatttt ttcattccgg atctgctcta tttgactgag cgccgtcaaa
    10081 tattgatcgt aagtagcggt agctgttact tgaccgtctt tcagcatcgt atcgaatagc
    10141 tgatctagtt gcgtgacttg tgcaatttgg gtatcagcga atcctagata ttgcataacg
    10201 actgttttcc aagcactatc cttaaaaaag ctcatatttt cacgtacatg ggcgacatct
    10261 gcacttgcca acttttcttt gatgatgaca tcatccacag cttgctgcca gtttgatacg
    10321 tttttagtaa ataatttatt gacagccgtc tggatcttca gttttgattc tgcatcctct
    10381 aactgcgtta agactgcttt cttttcttct tggatcgcag cagcttcttt aggtaattct
    10441 gattccttga tgtcataatc atctgccgaa agtttgatag aagatagttt atgtgacaaa
    10501 tttgaaactg tttcttctgt tatttcagga tttaggaaaa ttttttcttt tgtactgtac
    10561 agcttatcta cttcttttga taaagaggtc acggtgtctt tttgattggt gatggcagat
    10621 tctgcgtaat tgatccgttg atgatattgc cgctgattat tgagatggta tccaaacaag
    10681 caaatcacaa tagcgcctgt gcctaccgcc acagtagcca ataacttgac gaacggcttt
    10741 tccatagatt cactcctaaa tattgatgat agataagtaa agatgtgatt cattcacatc
    10801 agaatgagcg ctttttattt taaaagcata attaaactaa tatattcata gaaatattta
    10861 taaacaggtt ttccctatat aatcatacca catacagttt ttttgactag ggaaattcct
    10921 aatttaaaag taaataaaac agtaacaaga ttataattaa tcgttttcat ttttatttcc
    10981 tcacttttca tgagcgtttt gaaaattcga acaaagaata aaataacaaa aattattaaa
    11041 atagaattaa taacagctat ttataacaaa aaaaacagag acacagcacg tttcgtgcat
    11101 gctctctgtt tttcaaacaa aagactattt tgtgatgatt tgatccaagc ttgctaatga
    11161 caaagccatg gcagctattt gacttagttg tgttagtcgg ttcttacgta ctgctggttc
    11221 atcagccatg accatcgttt gatcaaaata attttcgatc agtggacgaa gagcgacaag
    11281 ctggtcgtag ttttcgtgga tcgtttggtc agcaaaactt gctttcattg cttctactgc
    11341 tttgtgtaat tctttctctg cgtcgttttc gaacagctca gggtctactt ctgattgttt
    11401 ttcaagctgc gcttttttcg ctaagttcac gacacgtgtc aaagcttcca ttgaaggttt
    11461 gaagtcttca tctgcaagat gttgtttgaa caattgtgct gctgcaaata cttttgtcag
    11521 atctttttgt tccgcattca aaacagcatc gatgacatca tgacgaatat tcttcgttgt
    11581 caataattgg cgcagacggc ctttgataaa gtcgatcact tgttgttgcc cttctgataa
    11641 accaatgccg aaacgatccg tatctttact gataacatcg ctaatcgttt cttgcagaac
    11701 agacaaagga aaggcccatt ttttcgattc gatgatccga acgacaccgt atgcttggcg
    11761 acgtaaggca taaggatcgt ttgacccgct tggaatcatc ccaactgcaa agaaagaaaa
    11821 gatgctatct aatttatctg caatcgctaa aacagaacct actgtagact gaggtagctc
    11881 tccttcactg gatataggca tgtaatgctc acgaatcgct gtcgctacag caggtttctc
    11941 gcctttcaga agagcatatt tttcgcccat gatcccttgt agttcaggaa attcccctac
    12001 catgttggtt actagatcaa atttatagat ttcagcagct cgttgcaaat ccatcaattc
    12061 ttcctcagat agtcctactt gttcgccaat gatacgggca atgacttgta cccgttgcat
    12121 tttctcatag atactaccga ttttttcatg gaaagtaaca gtttttagtt tttcaacgta
    12181 ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt ctaaacgagc
    12241 cgtcaatact ttttgattgc ctcgaaccac atcttccaat tttacccgat caccattacg
    12301 agcagaaata aaatgaggaa gcagcatacc ttgatcattc cgtacctcga aataacgttg
    12361 atgttctttc atagaagtga ctagtacttc ttcaggaaca gaaagatatt tttcttcgaa
    12421 gtcgccagaa aagacagttg gatattcgac taaattgttg acttcttcta gtaaatcttc
    12481 gtctagatca ataacccagt gatttttttc tgctaattcg tttgcttgtg taacgatcat
    12541 ttcttgtctt ttttgcggag acgcaatgac gaattgttca ttcaattttt cttcatattc
    12601 ttttgcatgc gcaaatgtca catcttcgcc taagaaacga tgacctcgag aggctctgcc
    12661 tgttttgaca tccaagattt cgaatgggat tacttcgtca tctaacaatg ccacgatcca
    12721 gtgaataggg cggatatatt caaaatcgta ttctgcccaa tgcatcgtta cagggaaagt
    12781 caaacttttg atcacccctg ctaagcctgt caatacttct tttgcttcct taccatgagt
    12841 gtatttcgtt acatagacat attccacacc ttttaattct ctaaaagtga tagcgtctgt
    12901 cgtcagacct tgtccgcgga caaatccttc cgctgctttc gtccagtttc catctgcgtc
    12961 caaagcaatt tttttggctg gtcctttgac ttcttcttca ctgtccgctt gtcgttctgg
    13021 aatatttgtt actttgattg ctaaacggcg cggtgtggaa aatgtttcga tcgtatcata
    13081 agtcaattgg ttttcatcta aaaattttgc tgttttttct tctaattgtt tcatgcttgg
    13141 tgtgacaaca tgggcaggca tttcttccag cccgatttca agtaataagt cttttgccat
    13201 cttattcagc ctccttcacg gtttctggat ttgctgtgtt tttattcaac aaagggaatc
    13261 ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg ttgcggatgc
    13321 gagaaagata accggcacgc tctgttacgg atactgcacc gcgggcgtct aataagttga
    13381 atgtatggct acatttcaag atataatcat acgccggatg aacaagattt tcatcgatac
    13441 agcgtttagc ttctttttca aaacgatcaa agttgcttag tagcaagtct tggtcgctta
    13501 cttcaaaaga atatttggaa tgttcatatt ctggttgttt gaagatctcg ccatatttca
    13561 cgccatcagc ccattctaaa tcatagacac tttcaacttc ttggatataa gaagcaagac
    13621 gttctaatcc ataagtgatc tcagaagtta ctggtttaca aggcagtcca ccaacttgct
    13681 ggaagtacgt gaattgtgtg atttccattc cgtcaagcca tacttcccaa cccacaccgg
    13741 cacagcccat ggatgggttc tcccagttgt cttcaacaaa acggatgtcg tgttctaatg
    13801 gatcgattcc taaaaggcgc aagctttcta aataaagctc ttgaatattc tcaggtgaag
    13861 gtttcatcac cacttggaat tgatgatgct gatacaaacg gtttgggttt tcaccataac
    13921 gtccatcagc tggtcggcga gatggctcta cgtaagcagc attccatggt tctggtccga
    13981 ttgcccgtaa aaaggtatat gggctcatcg tacctgctcc tttttctgtg tcataagctt
    14041 gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc atatcttgga
    14101 cggtcaattt tttactcatc tgttttgccc ctttcaattt taaagcgttg ctgtcaaata
    14161 agaatacagg aaagaaaacg acaaaaaatc cctatgtctg attacagaca tagggacgaa
    14221 taattcgcgg ttccacccta cttccgaata aagattcggc agcttcattt atcgtactcg
    14281 caagtgcctc ttcaaaaatc gtttgggtcc ggctcccact ctcccgaact cgctaaacca
    14341 cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc ttgtttctat
    14401 aatagatcct atcactgaac gatctattta atctatggtt aataatagac ggaactccct
    14461 gtgtttgtca aatggttttg attttttgtt gtatgtttta tttactattc ttctttttca
    14521 ggtgtctctt tttctttttc agacatgtct ttctctgacg tctttaatac gtgtgcccaa
    14581 gacttcattt gattgatgaa ctttttgctt ttcaaatgga taccgacata ttcatcatac
    14641 agctggtcga tgagttcacg gattgcttgt ttggtttctt ctttcagatg gattccacta
    14701 attttatcat aagaaatagc tgaaaacatc cgaacgaaat aaacagctcg aggatctgca
    14761 tgataacggt gatcgtccat cgaccaatgt ctttcgcata tcacaccacc atatttggaa
    14821 gaatagtcaa attttccttt tgtttccccg catacgacgc agtgtgtcca gattggtgca
    14881 attccgaagc gttgcagtat ttggacttca aaaatattcg tgatgatttc tgcatcgtct
    14941 ccttggtcca tccgttgcag tgcttgttgc gtaaactgga acaaatgcgg gtcgtactga
    15001 tgatcttcga ttgccgcatc tactagattc aataaatatg tgccatagcc gctcaaaaag
    15061 atgtcttctt ggatgtgtcg aaacgcatgg acatctttga cactgcttaa aaatgacaaa
    15121 ccttcacttc gaaaattccc gatataaact gcttcagtaa aagggagcaa agccggagct
    15181 aagggctgat tttgccgatg gatctctttg atataaaaca tcattttgcc agaagattct
    15241 gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa aatcaagcct
    15301 tttgattctg caagctggct catattctcc ctcctaaaaa ataaatctgg aaaagcagat
    15361 tctgccgatc cagatcattt atgttgcgat cttccgcagc ttttctcaac acgcggattt
    15421 ttattcagtt tatttctata cctaatttac aagaagctca gaatatcagt attcgtcttt
    15481 tcggtagccg aagtcttgca gatagacttt tttgtctcgc cagtcttttt gaactttgac
    15541 ccaaagttct agaaagactt tgtctcctaa aagattttcg atatcttttc ttgctttcgt
    15601 tcctacttgc ttcagcattt ttccgccttt gccgataatg atcccttttt ggctatctct
    15661 ttcgacaata atcgtagcct ggatatggat tttatcattc tcgtttcgct tcatcgaatc
    15721 aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga ctttctcacg
    15781 gatcaattca gaaacgatga agtattcagg atgatcagtc acttgatcat ctggaaaata
    15841 ttgtgggccc tctggcattt cactgactaa tacatccatc aaacgttcga cgttgtttcc
    15901 ttctgtagca gagattggaa tcacttgtgc aaaaggcatc tgcgttgaat aatcttcgat
    15961 gattcccaac aactcgtctg gatggatttt gtcgatttta ttgataatca gataaacagg
    16021 agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccgaccgc gtttttgatc
    16081 agcactaatc ataaataaga ccgcgtccac ttctttcaac gcactgtaag ctgcttctac
    16141 cataaaatct cctaaacgat gctttggttt atggattcct ggtgtatcaa taaaaatcaa
    16201 ctgagcttct ggtgtagtgt atactccttg gattttattt ctagttgtct gtgccttgtc
    16261 actcatgatg gcaatttttt gacccacgat tcgattcaat aatgtggatt tccctacatt
    16321 tgggcggccg acgattgcga caaaccctga tttatgttct ggcatatatg ttctcctttt
    16381 taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaac gatgacggta
    16441 aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt cccaatcgga
    16501 tgaaaatgaa aatccgtaaa catgtccacg acattttcaa aaacagtatt gatgatctct
    16561 actatccata cgagaaaaac agctaaaagc agccataacc attctatccg atcaagttgg
    16621 aaaatcacgc ccataaacaa agcgaggatt ccgaacgcaa catgtttcct catgttgcgt
    16681 tcttcctcaa aaacagtttt gactccttgg acggcaaatt ccacggacat caggaaatgt
    16741 ttgtttttct ctacgttttt atcttttaag tccataggca tctaagatct ctttctgtaa
    16801 accaaacatt tctttctcat cctctggtgt catatggtca tacccgttga tatgcaagaa
    16861 tccatgaacc gctaaaaagc caagttcccg atcatacgaa tggccgtaag cttctgcttg
    16921 ctctgcagca cgttcagtag agatcatgat gtctcccagt acacgaggca atggattctc
    16981 tccatcttca aaaatcactg ggatttcgtc ttcgccctct tcttccaagg caaaactgat
    17041 gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgga ttgctgcatt
    17101 gtccatgaaa gtaacagaca tttctgtttc ttctggcaac tctaaatatg atgctgcaaa
    17161 ttgcagcaag tcgtcgattt ctttcatttt ctcttctgag acttggtttg tttcatccat
    17221 gaaggtaata tccattatac atcgcttctt tctattcttc ttgctattct tcttgttctt
    17281 cttttatctt ctcaacttcc gatttcattt taggatattt gatccgagaa tggaaggtac
    17341 tgcttaaacc gctgatcaat gatttttcta ctttacggat ttcttttaac gtcaatccac
    17401 tgtcgtccaa ttgtccatcg gaaatacgct cttcaataag attatggaca aaactttcga
    17461 ttttttcgct cgttggatgg tccattgccc gtactgccgc ttcacaactg tcagctatat
    17521 tgacgattcc tgcttcacgg ctttgaggtt ttggtccagg ataacggaat tgctcttccg
    17581 taacttccgg gttgcgttcc tttgccttaa cgtagaaata acgcatcagc gtggttccat
    17641 gatgctgtct acaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg
    17701 caccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt
    17761 gcggattttc cgctcctgaa ggcaagttct caacgaaaaa gctggcgtgt ttgatcttgc
    17821 cgatatcatg ataatagcaa gctactcgag ttagtaaaga acgtccaccg atttctgcta
    17881 cagcattagc acttagattg gctaccatca tgctatggtg atacgtacct ggtgcttctt
    17941 ccagcaactg tttcaataaa ggatgattgg gattgctgag ttcatttaat acgatcacac
    18001 tatcatcatt gaccatcaat tcaatatagg ggtgcagacc tatcgtcagc aagaatgaaa
    18061 acagtgcacc tgtgaatccg cagactagcg ttaaccaagt cgttgcatcc ccaaagctca
    18121 ttccttggta gataatcaaa atgacatcca tcatcacagg aaaaataatg acccacatag
    18181 cagcggataa cccttgttct gagatccgtt tgcgtctgac gatcgttgcc atgagaccag
    18241 aaaatacata ggacatcaaa ataactgtca gaaagttcgt gccaatcgaa tcataaaaaa
    18301 tgaaaagtgc ggatactgct tgaaaaatac cagccatgat ccctgctctt cgattcaaga
    18361 aaaagcttaa aacaagtgga gcaaaagccg cggggtaaaa cagcggaata taagcagctt
    18421 gttctgtttg gaagagttgg aaaaatttca ttagtacgac gcttactgac attgccgtca
    18481 cgtagaaaag tacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa
    18541 tcagtacacc gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatagaag
    18601 tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat
    18661 ttccttcgcg aacaataatt tctccttgga agatcatcac tggttgtaca gcttctcgtg
    18721 cagattgttt taattcttct gtctttttct cattcaatac atcattaact acgatgcctt
    18781 gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa tattgaatct
    18841 gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct
    18901 gttcgtcaat gagcttcaaa ctttcatccc taactgtgtg aatttccgtt tcagtcattt
    18961 caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat
    19021 tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga
    19081 tattttcatc ttccttcgcc ttatctacct tgccattata agatttgttg atggtatcat
    19141 tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat
    19201 ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg
    19261 tttcttccgt gttctcaatc gttttattcg cacggatact ctcttctgct acttggcctt
    19321 ctctgaaatc aatggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa
    19381 aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca
    19441 gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc
    19501 cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc
    19561 aaaattaaca aaatcgattt ttttgatttg ctttaatgtt cgttctgcat gaacaagacc
    19621 acttgtcgtc ccacgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga
    19681 ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc
    19741 gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta aaggtgcgat
    19801 ttcgatcacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa
    19861 agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa
    19921 gcctaggttt tcccctgcct ccacagcggg ccgcgtcaaa atgatttttt gcacttcgcc
    19981 tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg
    20041 ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct
    20101 gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc
    20161 aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacaa catctggcgt
    20221 acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa
    20281 ggaaatctgg gattgctcac caatgatctg aatgatttct ccacgtgtat ggatggttgt
    20341 ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac
    20401 atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca
    20461 tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca
    20521 aataagaaaa ggttgtaaaa gagtcgctta accatacgta ccaagaaacg ttaaaaaata
    20581 gccgtttcca ttcgctaaag gtctcgtttt gataaaaaga agctgagaat gtaagttata
    20641 tgaaagtgga gaacaaaata tttcgtttca ttcaacaagt attctctagg tatttttcct
    20701 gtttcaaaaa aagaaaacaa aatagttaaa atattcttgc atttcaaaaa aaacaggtgt
    20761 attattacgt caacaaataa acatacagca aagcgtcgag gaaaacagta ggctattcaa
    20821 cgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat agacgaacat
    20881 catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta tcaggctagc
    20941 ggataacttt atcaaaaagc gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg
    21001 taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt ttattttaag
    21061 gtggtggttt tcatggaatc tgatattagt ggcattacta attgctgcaa cgtttatatc
    21121 gtcaatcaaa atgataatta ttggaggaaa aatttatgga agaaacaaaa acttattcat
    21181 ggaaacaact attgatggtc agttcactta tcttcgggat gttcttcggg tcaggtaatc
    21241 taatctttcc tgtccactta ggtcaaatgg ctggcgcaaa ctgggtcagt gctggcgtag
    21301 gatttgctat ctcaggtgct cttttcccat tactagctat cttagctgta gtagtgacaa
    21361 aaagtgatgg tctgtatgac ttagctaaac ctgtcggaaa atggtatgct gccttgttct
    21421 taatccttgt tcatttgacg atcggaccat ttttcggtac accacggaca gctgctactg
    21481 cttttgaaat ggctgctaaa ccatttttgc ctgaaaaata taattcatta ggtatgtttt
    21541 tattctctgc tcttttcttt ttacttgctt atttccttac tgtccatcca acacggttgg
    21601 tcaaatacgt cggaaagtat ctaaactcct tgtttcttgc attattagca atcatttttg
    21661 tcgtttcttt attccatcca atgggaaatc taaatcaatc tgctgcagat acttatcaaa
    21721 caaacgcttt aacaaatggt gtcctagaag gttataacac ggttgatgca gtagctttgc
    21781 ttgccttaag tgtcactttt gtccatgcag tccgtgacct tggtttcaaa gataaaaaag
    21841 tcacagaatt aacggcaaaa gctggtagcc tgagcgttgc gctagaagtc atgatctatt
    21901 ttggacttgt tcttctcggt gcgatgagtt tgaataagat gtctttatca gaaaatggcg
    21961 gaactgcact ttctcagatc actaattttt atttaggtaa cttcggcgct agcttcttag
    22021 gagtgatggt cacattaggt gtcttcacta ccgctatggg actcgttgtt tcttttgcgc
    22081 aagatttcca taaactgttc cctaaagtca gttatatgac atggctgcgg ttaacgactt
    22141 tcgtctcttt tgtcgttgca aatgctggtt tagacaatat catccaatgg tctttaccag
    22201 tattgatgct tttgtatcca ttgtcgcttg cgttgatctt gctttcctta acagcaaaat
    22261 ttttccaaaa gacaccattt gtctatcaag taacgatgtt gtttgctgct gttcctgccg
    22321 tattggatat gctggcaaat tcgcctgctt tagtgagtca acaaagagtt gtagcaagca
    22381 tgctcgaatt ttatcatcat cacgttcctt ttgctgcatt aggacttggc tggatggtcc
    22441 caacgttact tggttacgct ggcagcctac tgttttatta cgcctatcgc ctatcagggt
    22501 ataaacaaga agcaaatgaa ctaccagaag aataaccaaa tcaaacacaa tttaacataa
    22561 gaaatattaa acaggaaaaa accagctaga tgaaagaatc ctattctttc gtctagctat
    22621 tttttagttc tcttttttac gcgcacaaaa aaactgcgga caatgattgt ccgcagttta
    22681 aatgggctct ataatatcta gtgttggtga cgccttctgt tgccaatact ttttcttttt
    22741 tctaataaac gcaaacagac accttgaaat cctttagaat aaagtcgacg aaaaccattc
    22801 aaaggagtgt ttcaagatgt cctattcaga gtttaccaaa gagttactag atattctaga
    22861 cctaaatctt atctttcatg aagatgcctt tcgcaaagag cgaatcaatg acgaaacatg
    22921 ttttgttttt gatggcacat tgacctatca accagaggaa tgcttccatt gtcactatca
    22981 aaataagcaa acgatcatta aat
//
LOCUS       DAZKPO010000051        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000051 DAZKPO010000000
VERSION     DAZKPO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.051"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG8NZ_001822"
     CDS             complement(11..1942)
                     /locus_tag="ACG8NZ_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0539487.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8NZ_001823"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8NZ_001823"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0539488.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG8NZ_001824"
     CDS             complement(2871..3623)
                     /locus_tag="ACG8NZ_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0539489.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG8NZ_001825"
     CDS             complement(3782..3991)
                     /locus_tag="ACG8NZ_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539490.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG8NZ_001826"
     CDS             complement(4002..5330)
                     /locus_tag="ACG8NZ_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539491.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG8NZ_001827"
     CDS             5466..5621
                     /locus_tag="ACG8NZ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0539492.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8NZ_001828"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8NZ_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0539493.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8NZ_001829"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8NZ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0539494.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8NZ_001830"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8NZ_001830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0539495.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG8NZ_001831"
     CDS             complement(9305..10237)
                     /locus_tag="ACG8NZ_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539496.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG8NZ_001832"
     CDS             complement(10394..11053)
                     /locus_tag="ACG8NZ_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0539497.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8NZ_001833"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8NZ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0539498.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG8NZ_001834"
     CDS             complement(12094..12384)
                     /locus_tag="ACG8NZ_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539499.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8NZ_001835"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8NZ_001835"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0539500.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8NZ_001836"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8NZ_001836"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0539501.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG8NZ_001837"
     CDS             complement(16049..17218)
                     /locus_tag="ACG8NZ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0539502.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8NZ_001838"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8NZ_001838"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0539503.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8NZ_001839"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8NZ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0539504.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG8NZ_001840"
     CDS             22182..22703
                     /locus_tag="ACG8NZ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0539505.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKPO010000052        22419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000052 DAZKPO010000000
VERSION     DAZKPO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.052"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8NZ_001841"
     CDS             6..305
                     /locus_tag="ACG8NZ_001841"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0539506.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8NZ_001842"
     CDS             378..1739
                     /locus_tag="ACG8NZ_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0539507.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8NZ_001843"
     CDS             1761..2021
                     /locus_tag="ACG8NZ_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539508.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8NZ_001844"
     CDS             2035..2787
                     /locus_tag="ACG8NZ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0539509.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8NZ_001845"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8NZ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0539510.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8NZ_001846"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8NZ_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0539511.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8NZ_001847"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8NZ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0539512.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8NZ_001848"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8NZ_001848"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0539513.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8NZ_001849"
     CDS             7161..8558
                     /locus_tag="ACG8NZ_001849"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0539514.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8NZ_001850"
     CDS             8639..9079
                     /locus_tag="ACG8NZ_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0539515.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8NZ_001851"
     CDS             9227..9553
                     /locus_tag="ACG8NZ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0539516.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8NZ_001852"
     CDS             9554..10303
                     /locus_tag="ACG8NZ_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0539517.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8NZ_001853"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8NZ_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0539518.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8NZ_001854"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8NZ_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539519.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8NZ_001855"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8NZ_001855"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0539520.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8NZ_001856"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8NZ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0539521.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8NZ_001857"
     CDS             14770..17436
                     /locus_tag="ACG8NZ_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0539522.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8NZ_001858"
     CDS             17800..18552
                     /locus_tag="ACG8NZ_001858"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0539523.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG8NZ_001859"
     CDS             complement(18574..19728)
                     /locus_tag="ACG8NZ_001859"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0539524.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACG8NZ_001860"
     CDS             19913..22354
                     /locus_tag="ACG8NZ_001860"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0539525.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3932 c         4672 g         6617 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttttt
//
LOCUS       DAZKPO010000053        22301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000053 DAZKPO010000000
VERSION     DAZKPO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.053"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG8NZ_001861"
     CDS             5..403
                     /locus_tag="ACG8NZ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0539526.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG8NZ_001862"
     CDS             387..1082
                     /locus_tag="ACG8NZ_001862"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0539527.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="ACG8NZ_001863"
     CDS             complement(1676..2563)
                     /locus_tag="ACG8NZ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0539528.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="ACG8NZ_001864"
     CDS             2748..3587
                     /locus_tag="ACG8NZ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0539529.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="ACG8NZ_001865"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="ACG8NZ_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0539530.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="ACG8NZ_001866"
     CDS             5055..5411
                     /locus_tag="ACG8NZ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539531.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5456..6571
                     /locus_tag="ACG8NZ_001867"
     CDS             5456..6571
                     /locus_tag="ACG8NZ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539532.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="ACG8NZ_001868"
     CDS             complement(6597..7037)
                     /locus_tag="ACG8NZ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0539533.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="ACG8NZ_001869"
     CDS             7197..7703
                     /locus_tag="ACG8NZ_001869"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0539534.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="ACG8NZ_001870"
     CDS             7871..9784
                     /locus_tag="ACG8NZ_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0539535.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="ACG8NZ_001871"
     CDS             9789..10790
                     /locus_tag="ACG8NZ_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0539536.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="ACG8NZ_001872"
     CDS             complement(10992..11198)
                     /locus_tag="ACG8NZ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0539537.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="ACG8NZ_001873"
     CDS             complement(11844..12692)
                     /locus_tag="ACG8NZ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0539538.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="ACG8NZ_001874"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="ACG8NZ_001874"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0539539.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="ACG8NZ_001875"
     CDS             13760..14761
                     /locus_tag="ACG8NZ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0539540.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="ACG8NZ_001876"
     CDS             14758..15042
                     /locus_tag="ACG8NZ_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0539541.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="ACG8NZ_001877"
     CDS             15075..15872
                     /locus_tag="ACG8NZ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0539542.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="ACG8NZ_001878"
     CDS             15886..16719
                     /locus_tag="ACG8NZ_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0539543.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="ACG8NZ_001879"
     CDS             16737..17594
                     /locus_tag="ACG8NZ_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0539544.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="ACG8NZ_001880"
     CDS             17616..18125
                     /locus_tag="ACG8NZ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0539545.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="ACG8NZ_001881"
     CDS             18162..18563
                     /locus_tag="ACG8NZ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539546.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="ACG8NZ_001882"
     CDS             18675..19028
                     /locus_tag="ACG8NZ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539547.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="ACG8NZ_001883"
     CDS             19062..19505
                     /locus_tag="ACG8NZ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0539548.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="ACG8NZ_001884"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="ACG8NZ_001884"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0539549.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="ACG8NZ_001885"
     CDS             20600..21313
                     /locus_tag="ACG8NZ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0539550.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21520..21669)
                     /locus_tag="ACG8NZ_001886"
     CDS             complement(21520..21669)
                     /locus_tag="ACG8NZ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539551.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22065..22265
                     /locus_tag="ACG8NZ_001887"
     CDS             22065..22265
                     /locus_tag="ACG8NZ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0539552.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7683 a         3427 c         4620 g         6571 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaaattt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa a
//
LOCUS       DAZKPO010000054        22268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000054 DAZKPO010000000
VERSION     DAZKPO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.054"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..535)
                     /gene="yajC"
                     /locus_tag="ACG8NZ_001888"
     CDS             complement(155..535)
                     /gene="yajC"
                     /locus_tag="ACG8NZ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0539553.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(632..1777)
                     /gene="tgt"
                     /locus_tag="ACG8NZ_001889"
     CDS             complement(632..1777)
                     /gene="tgt"
                     /locus_tag="ACG8NZ_001889"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0539554.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1995..2693
                     /locus_tag="ACG8NZ_001890"
     CDS             1995..2693
                     /locus_tag="ACG8NZ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0539555.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2861..5500)
                     /locus_tag="ACG8NZ_001891"
     CDS             complement(2861..5500)
                     /locus_tag="ACG8NZ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0539556.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6119..7150)
                     /gene="queA"
                     /locus_tag="ACG8NZ_001892"
     CDS             complement(6119..7150)
                     /gene="queA"
                     /locus_tag="ACG8NZ_001892"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0539557.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7343..8517
                     /locus_tag="ACG8NZ_001893"
                     /pseudo
     CDS             7343..8517
                     /locus_tag="ACG8NZ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8521..9162
                     /locus_tag="ACG8NZ_001894"
     CDS             8521..9162
                     /locus_tag="ACG8NZ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539558.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9159..10076
                     /locus_tag="ACG8NZ_001895"
                     /pseudo
     CDS             9159..10076
                     /locus_tag="ACG8NZ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            10073..10744
                     /locus_tag="ACG8NZ_001896"
     CDS             10073..10744
                     /locus_tag="ACG8NZ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539559.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11285..11773)
                     /locus_tag="ACG8NZ_001897"
     CDS             complement(11285..11773)
                     /locus_tag="ACG8NZ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0539560.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11974..12240)
                     /locus_tag="ACG8NZ_001898"
     CDS             complement(11974..12240)
                     /locus_tag="ACG8NZ_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0539561.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12240..12503)
                     /locus_tag="ACG8NZ_001899"
     CDS             complement(12240..12503)
                     /locus_tag="ACG8NZ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0539562.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13024..13164
                     /locus_tag="ACG8NZ_001900"
                     /pseudo
     CDS             13024..13164
                     /locus_tag="ACG8NZ_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13306..15129)
                     /locus_tag="ACG8NZ_001901"
     CDS             complement(13306..15129)
                     /locus_tag="ACG8NZ_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0539563.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15727..18432)
                     /locus_tag="ACG8NZ_001902"
     CDS             complement(15727..18432)
                     /locus_tag="ACG8NZ_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0539564.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18738..19685)
                     /locus_tag="ACG8NZ_001903"
     CDS             complement(18738..19685)
                     /locus_tag="ACG8NZ_001903"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0539565.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19875..20144
                     /gene="rpsN"
                     /locus_tag="ACG8NZ_001904"
     CDS             19875..20144
                     /gene="rpsN"
                     /locus_tag="ACG8NZ_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0539566.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20320..20682)
                     /locus_tag="ACG8NZ_001905"
     CDS             complement(20320..20682)
                     /locus_tag="ACG8NZ_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0539567.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20693..21814)
                     /gene="alr"
                     /locus_tag="ACG8NZ_001906"
     CDS             complement(20693..21814)
                     /gene="alr"
                     /locus_tag="ACG8NZ_001906"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0539568.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21830..22180)
                     /gene="acpS"
                     /locus_tag="ACG8NZ_001907"
     CDS             complement(21830..22180)
                     /gene="acpS"
                     /locus_tag="ACG8NZ_001907"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0539569.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         6306 a         4763 c         4144 g         7055 t
ORIGIN      
        1 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
       61 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
      121 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
      181 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
      241 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
      301 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
      361 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
      421 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
      481 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
      541 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
      601 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
      661 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
      721 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
      781 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
      841 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
      901 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
      961 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     1021 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     1081 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     1141 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     1201 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     1261 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     1321 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     1381 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     1441 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     1501 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     1561 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     1621 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     1681 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     1741 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     1801 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     1861 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     1921 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     1981 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     2041 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     2101 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     2161 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     2221 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     2281 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     2341 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     2401 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     2461 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     2521 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     2581 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     2641 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     2701 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     2761 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     2821 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     2881 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     2941 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     3001 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     3061 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     3121 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     3181 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     3241 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     3301 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     3361 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     3421 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     3481 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     3541 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     3601 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     3661 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     3721 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     3781 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
     3841 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
     3901 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
     3961 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
     4021 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
     4081 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
     4141 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
     4201 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
     4261 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
     4321 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
     4381 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
     4441 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
     4501 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
     4561 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
     4621 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
     4681 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
     4741 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
     4801 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
     4861 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
     4921 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
     4981 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
     5041 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
     5101 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
     5161 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
     5221 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
     5281 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
     5341 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
     5401 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
     5461 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
     5521 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
     5581 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
     5641 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
     5701 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
     5761 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
     5821 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
     5881 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
     5941 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
     6001 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
     6061 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
     6121 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
     6181 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
     6241 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
     6301 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
     6361 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
     6421 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
     6481 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
     6541 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
     6601 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
     6661 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
     6721 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
     6781 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
     6841 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
     6901 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
     6961 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
     7021 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
     7081 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
     7141 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
     7201 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
     7261 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
     7321 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
     7381 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
     7441 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
     7501 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
     7561 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
     7621 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
     7681 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
     7741 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
     7801 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
     7861 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
     7921 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
     7981 tgtgatcaat ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa
     8041 gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag agtcggcgaa
     8101 gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga agcaatcaaa
     8161 ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa tgtattaaaa
     8221 ggatttatcg atgtagaaac gttgaataaa aaacgaggaa aagtatccag tgtcggcgac
     8281 attttgaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa
     8341 cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta
     8401 ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg gggagaagat
     8461 cccgcaacta ctgatgttgc agagtcagct actcctgaag aatctaagga ggtctgacct
     8521 atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt tgaacatctt
     8581 tatatttcag gacttgcttt gatcattggt atcatcattg ctgtaccgct tggtgtctta
     8641 ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc cttgcagaca
     8701 atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg taaattacct
     8761 gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac gtacattggg
     8821 atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat gactgagttc
     8881 caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc tggtattcgt
     8941 ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg cgctggtggt
     9001 ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat tttggcaggt
     9061 acgatccctg taacaatttt agcactattg gctgattggc ttctaggtct cttggaacac
     9121 cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata ctaaaaatga
     9181 ccatggttct tttctctctt cttatacttg cgagttgttc cttccctggt cttgccagca
     9241 atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca cagatcttag
     9301 caagtctagt tgccggaatg gtttaacatt atacagataa aaataccgca atcatcaaca
     9361 atctggctac tacaactatc aaccatcaag cgatgatgaa cggagatgct tcaatttcag
     9421 cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct gaaaaagatc
     9481 cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacggt caaacgtggt
     9541 tcccgtccta tagttttgaa aatacgtatg tctttcttgt tcgaaaagac accgcacaaa
     9601 aataccatct atctaaagtc agcgatctga aaaatgtagc ggatgaatta gtagctggtg
     9661 tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa gaaacttatg
     9721 gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac gcagtcgaag
     9781 caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc agttatgatc
     9841 tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct ccagttgtag
     9901 acaacaagat attagctgat acgcctcatt taaaagaagc cttaaataag ttaggtcata
     9961 ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat ctagtggaac
    10021 catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg aagtgaagca
    10081 attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa atggctccta
    10141 tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc tctttgcagc
    10201 agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag caaactgggt
    10261 gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct ccattttaat
    10321 cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt attccctctt
    10381 accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca tccttgatgt
    10441 cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt taccactctc
    10501 tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg ggataaccgc
    10561 gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag gaaccaatgc
    10621 aacagagggc acatcgatta ttttagcagg tgcattgcca actgcattaa tggcgatcat
    10681 cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc gtacatcacg
    10741 ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca aaggcttttt
    10801 tagttctgtc atttttaagg caataatcca ctctgattct tccctcctta tgggcaaaga
    10861 gttgcttttc ttagctattt atccattccg tatactccaa cttcatactg ccaacgggtt
    10921 catatctgca tccaccgact aagttttttt cttatccgct aaggcatttt gccaatccat
    10981 tttcccatca ctcgtcatgg aattcatacc acatgatctc taaatactcg tctgcttgtt
    11041 ctttgaattg ggcatcaatt cttacatcct ttggcgtggc tttcatttaa gccttttcca
    11101 tcagtcctcg attgttcgat gccttcagct gcatcatcaa aactggtgac tgccgtatcg
    11161 gtaacttcga tcatctttta tacaatcggg aacttgccat cctcatcctt ggctaaaaac
    11221 atatctagcc attccagctt ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg
    11281 attcttatgg actgcatccg tcgcaactag acttggcgat acgatgatcc ttcaccagaa
    11341 gagcaccccc gtaaaccaat tgatcgatat attgccaata agaaaagaca ttggcgtaaa
    11401 agatatcgat ccctcgtgat gcattgccgt tgatactgct attcatggcc gaaagcgcca
    11461 cgactttaac ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct ttggataccc
    11521 gctctgctaa aggaacgaca ctggtattgt atttcgtatc tgtatctttt ccaaaagacc
    11581 atgccaacga atcctctggg aaccaataag acgactccgg ttgattgtcc attgcttttt
    11641 tcaacatact tttggctgtt tctcgataaa cgacatttcc aattgtcttc gcctcacttt
    11701 ggcgataatc gatggtcgtt gccactgcca acactaacaa cgatgctcca attttactga
    11761 ttttccttgt cacacttctt cctccttgtt ttacataaac ccgtcaacat tccattccta
    11821 aactcaaaca atgacccttc aaaggtggag atataggaac aaattaaaga aatcatgcaa
    11881 taaatccaag aaggttggtt ttagaacaaa aaaagagtct cctttttggg gagactcttt
    11941 gttaactatc taaaaaggtt ggctcttctc acttcactca taatgtgtcc aggcagaata
    12001 aatttctaca actttatttt cctcatcaac tttgtaaacc actcgatgct gtctattcag
    12061 cctgcgtgag tatctgcctt catgtgtagg tctcagcttt tcaaaagact gcgtgggcat
    12121 atatggatct tccttcaatg tctgaatcac ttcttcaaat tgtttcttta aattcgtttg
    12181 cttgattttt cttaaatcca ctttcgcaga gtttttgatt gcgactgtat agttactcat
    12241 caaagagctt cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg
    12301 tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc
    12361 cagtcttcca gcccaatcaa tacagcattg tttccactac gatcactaat aatctcgatt
    12421 tcagtatgat tttcatttac ctctttcaat aactgataaa aatttttacg agcagtgcta
    12481 ggattcatcg ttgtattagc cattgtctct cctccttttt ctgatataat tatagcgtac
    12541 ttgattaagt acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac ttttcctctt
    12601 ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct cacttcttac
    12661 tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat catctttagc
    12721 aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga aggttcctcg
    12781 ttgcagtgtg acttgattga tatcttgaat aacctgagga tacgcacgaa agatttgtaa
    12841 cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc
    12901 tttcgtctcc tgtcattcat gttcactatc gatttgatac ttgcttagac cgttgggttt
    12961 gtgaatcacc aattaaaaat ggcactgagt tatggtaaca aaaaatggtc acttgacgag
    13021 gctgagattc ctacttatga agacacatgg cacttaagag tcgaggtaaa ggaagaagct
    13081 gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga tcaagacatc
    13141 aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac gctgttccca
    13201 tctttaatgg cttatagagg gcacctttag ctgagaataa gacaaaacag accctctagc
    13261 ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt tcttcaaatg
    13321 gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc ttaaccgcaa
    13381 acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa
    13441 cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta cataacggat
    13501 aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga catctgggaa
    13561 atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt ctagcgaaac
    13621 atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac cgatatttac
    13681 ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat gtagttttgc
    13741 atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga tcttataaaa
    13801 aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta accgatagac
    13861 aaacaaaatc gcaatgattg catatgagat gaacgcaccc gtaatattgg caatcgattt
    13921 gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt gagataaagc
    13981 aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg agccatggaa
    14041 aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg aatagcctag
    14101 tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca gattgtatgc
    14161 aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca gcgctgtagc
    14221 aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa tttgagacaa
    14281 aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg tgatccccac
    14341 gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat tttttgctct
    14401 tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta aagaggaaga
    14461 accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag ccgctcctgg
    14521 aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac caacaacgat
    14581 cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg atgcagattc
    14641 cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag aaatagcgac
    14701 ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg ctgagacaga
    14761 tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca agccagcatt
    14821 ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat aagcatggat
    14881 gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag cggcaatggg
    14941 tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga tttgttccgt
    15001 accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag caaatcgggt
    15061 taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca acctttttaa
    15121 gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat agtcgaaaat
    15181 ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata aactttaaga
    15241 aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt aatacagcaa
    15301 aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg atagaacttg
    15361 acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag agatgtaagg
    15421 ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac aacctcttga
    15481 taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta aaaacataag
    15541 aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt tacaacctct
    15601 tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg gcaaaatatg
    15661 aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat ttcacaacct
    15721 ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg tcattttttc
    15781 ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca aaccgatcat
    15841 gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt cccgtaacaa
    15901 gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt tccctgagac
    15961 ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga taatagcgat
    16021 ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca tgaacgctaa
    16081 ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta ctcctagtaa
    16141 gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag
    16201 catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct ttcctaaata
    16261 gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac atagtgcagt
    16321 ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat tggtttgtag
    16381 ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca ataatttgat
    16441 gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg ctttttggat
    16501 accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt
    16561 ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt cattttttag
    16621 tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct gaatgatcgg
    16681 taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg tatccatatt
    16741 tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca tttctttttg
    16801 atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc tgagaagcga
    16861 atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat
    16921 gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga taccagcaat
    16981 gatgccattg atattattaa caacggcatc aatttgatct agcaattcat caatagcctt
    17041 cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca gattgcttag
    17101 caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat ttccagcact
    17161 tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt gttgagatat
    17221 tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat cagtcagctt
    17281 accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg
    17341 tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag
    17401 ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt
    17461 cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc
    17521 gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg
    17581 cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg
    17641 atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac
    17701 tttagggatg taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag
    17761 ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc
    17821 tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta
    17881 acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt
    17941 gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat
    18001 tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag
    18061 cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac
    18121 tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg
    18181 aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt
    18241 gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc
    18301 tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt
    18361 tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt
    18421 gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt
    18481 tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa
    18541 ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta
    18601 tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg
    18661 caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt
    18721 ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt
    18781 ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa
    18841 ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca
    18901 tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa
    18961 tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg
    19021 ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca
    19081 aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt
    19141 accttttcct catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga
    19201 agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc
    19261 ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt
    19321 ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac
    19381 acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga
    19441 acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca
    19501 taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa
    19561 ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga
    19621 tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt
    19681 cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat tttgacaaaa
    19741 aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa
    19801 cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt
    19861 ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa
    19921 cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct
    19981 tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg
    20041 gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag
    20101 cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta
    20161 atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt
    20221 cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc
    20281 ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag
    20341 tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc
    20401 caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag
    20461 gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag
    20521 cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt
    20581 acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac
    20641 tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt
    20701 gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta
    20761 attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca
    20821 cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac
    20881 gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg
    20941 gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat
    21001 atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag
    21061 aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat
    21121 taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata
    21181 aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca
    21241 cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag
    21301 aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg
    21361 cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta
    21421 gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca
    21481 accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt
    21541 cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga
    21601 ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag
    21661 ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc
    21721 cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca
    21781 ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca
    21841 aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt
    21901 catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt
    21961 gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt
    22021 aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt
    22081 catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc
    22141 taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata
    22201 aatttagaac actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa
    22261 taacaaaa
//
LOCUS       DAZKPO010000055        22190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000055 DAZKPO010000000
VERSION     DAZKPO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.055"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..2095
                     /gene="tdc"
                     /locus_tag="ACG8NZ_001908"
     CDS             260..2095
                     /gene="tdc"
                     /locus_tag="ACG8NZ_001908"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0539570.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2400..3878
                     /locus_tag="ACG8NZ_001909"
     CDS             2400..3878
                     /locus_tag="ACG8NZ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0539571.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3844..5175
                     /locus_tag="ACG8NZ_001910"
     CDS             3844..5175
                     /locus_tag="ACG8NZ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0539572.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5289..7841
                     /locus_tag="ACG8NZ_001911"
     CDS             5289..7841
                     /locus_tag="ACG8NZ_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0539573.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7848..9287)
                     /locus_tag="ACG8NZ_001912"
     CDS             complement(7848..9287)
                     /locus_tag="ACG8NZ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539574.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9262..9444
                     /locus_tag="ACG8NZ_001913"
     CDS             9262..9444
                     /locus_tag="ACG8NZ_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539575.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9633..9995
                     /locus_tag="ACG8NZ_001914"
     CDS             9633..9995
                     /locus_tag="ACG8NZ_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0539576.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10182..11039)
                     /locus_tag="ACG8NZ_001915"
     CDS             complement(10182..11039)
                     /locus_tag="ACG8NZ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0539577.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11159..12070
                     /locus_tag="ACG8NZ_001916"
     CDS             11159..12070
                     /locus_tag="ACG8NZ_001916"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0539578.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12077..13723
                     /gene="mdcA"
                     /locus_tag="ACG8NZ_001917"
     CDS             12077..13723
                     /gene="mdcA"
                     /locus_tag="ACG8NZ_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0539579.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13825..14124
                     /locus_tag="ACG8NZ_001918"
     CDS             13825..14124
                     /locus_tag="ACG8NZ_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0539580.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14114..15790
                     /gene="mdcD"
                     /locus_tag="ACG8NZ_001919"
     CDS             14114..15790
                     /gene="mdcD"
                     /locus_tag="ACG8NZ_001919"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0539581.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15794..16387
                     /locus_tag="ACG8NZ_001920"
     CDS             15794..16387
                     /locus_tag="ACG8NZ_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0539582.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16411..17487
                     /locus_tag="ACG8NZ_001921"
     CDS             16411..17487
                     /locus_tag="ACG8NZ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0539583.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17512..18381
                     /gene="mdcB"
                     /locus_tag="ACG8NZ_001922"
     CDS             17512..18381
                     /gene="mdcB"
                     /locus_tag="ACG8NZ_001922"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0539584.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18492..19256
                     /locus_tag="ACG8NZ_001923"
     CDS             18492..19256
                     /locus_tag="ACG8NZ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0539585.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19745..21838
                     /locus_tag="ACG8NZ_001924"
     CDS             19745..21838
                     /locus_tag="ACG8NZ_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0539586.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21863..22165
                     /locus_tag="ACG8NZ_001925"
     CDS             21863..22165
                     /locus_tag="ACG8NZ_001925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0539587.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7183 a         3789 c         4728 g         6490 t
ORIGIN      
        1 tgaaggacag tcattttatt tgtttgttcg ctttttttag gttatctatt ttagaagact
       61 gatttattga aaaattgttc gcttttttgc gttgaattat cagaaaaatc cgactttatt
      121 tatttttgtt tgtaaaaatc tacgtggatt tgatgttata aaaaaaaaat tctcattttt
      181 ggcgctatag tgaagaggtg ttacagataa acacatgtat ctgcaagaca ttgaccaaca
      241 aaatatggag ggaagaaaaa tgaaagatat ggatatcaag gccgtcttta taggcgacaa
      301 agctgaaaat ggaccagttt ataaaatgtt gttgaacaag atggtagatg aacatcttgg
      361 atggagagaa aattatattc cttcagatat gcctgcaatt agcgaaggag ataagctcac
      421 tccagattat ctagccaccc gcgaccatat gatagaggtc ttggatgaag tcagtcagcg
      481 attacgcgca ggatcgatcc catggcattc agcaggccgt tactggggac aaatgaatgc
      541 tgaaacattg atgcctgctt tattagctta caactatgcg atgctgtgga acccaaataa
      601 tgttgcattg gaatcttcta tggcaacttc gcaaatggaa gcagaagtag gacaggattt
      661 tgcttcatta ttcaatatga cagacggatg gggacacatt gcagcagatg gttctattgc
      721 taatcttgaa ggattatggt acgcccgttg catcaaatcg attcctttag ctgtcaagga
      781 agtgctgcct gaaaaagtga aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga
      841 agaaattttg gaaatgaccg aaagctttac ggacgaagaa atggatgagg tcaaagcagc
      901 ttcttcccgt agcggaaaaa atatccaaag attagggaaa tggctggtcc ctcaaacaaa
      961 acactattct tggatgaagg cgttagatat ttgcggagtc ggcttagatc agatggttgc
     1021 tatcccagtt caagaggatt atcgaatgga tattaatgct ttggaaaaaa caattcgtga
     1081 attagctgga caaaaaatcc caattctagg tgtggttgct gtcgtaggaa cgacagaaga
     1141 aggtcaagta gacagtgtag ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg
     1201 gatctatttc tatttacatg ttgatgccgc atatggcggc tatgcgcgtt cactgtttct
     1261 aaacgaagca ggagaatttg tgccgtatgc ttccttagct gaattctttg aagaacatca
     1321 tgttttccac cactgcgtaa cgattgacaa agaggtatat gaagggttca gagctatttc
     1381 agaagcggat tctgtcacga tagatcctca taagatgggc tatgtccctt atgctgctgg
     1441 aggaatcgta atcaaacata aaaatatgag aaatatcatt tcttatttcg cgccatatgt
     1501 gtttgaaaaa tcagtaaagg ctccagatat gttaggagct tatattttgg aaggatcaaa
     1561 agctggagca accgccgccg cagtctggac agcacatcgt gtattgccat tgaatgtgac
     1621 cggatatggt caattgattg gggcttcaat cgaggcagct caaagattca gagaatttct
     1681 cgatcatctg acttttactg ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc
     1741 tgattttaat atggtcaatt gggtattcaa agaacaaggc tgtacagatt tgaacgctat
     1801 caatgaatta aatgaaaaaa tgttcgatcg gtcttcttat atggatggcg atgtttatgg
     1861 tgaacggttt attacttctc atactacatt tacacaagaa gattacggtg attctccgat
     1921 tcgttttgtt gaaagaatgg gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac
     1981 tctgctacgt gcggctatca tgactcctta tttgaatgat gatcgaattt tcaattttta
     2041 tacaaaaaaa atcgccaaag caatggaaaa gaaactaaac gaaatcatcc aatagaactg
     2101 atttttgtgt gaaagaataa caagagacat atggagaaca gacaattatt tgcttcatca
     2161 aaacaattgt atttccccct gtttttcata tattcctcgc caagatattc ttttttataa
     2221 aacgttctat aagttggatc gttcgtttca tgtatcaaac ccagttcact aagatcgttc
     2281 aatggatgtt tcggtgatac tgggttttgt actgaatcga tgaaaccttg tcatttctgt
     2341 tcttgaacaa aaataaaaga aaacaaaaac atatgtcgct attgaaagaa ggaataaaga
     2401 tgaaagatac tggttcatca aagcaaatca cgaccatgac ctttatcgga atgacctgcg
     2461 cccttgtagc aagtgtacga aatattccag atgtagcagc aacgagctgg acgatgattt
     2521 tttacatgct ggttgcagta ttattgtacg cttttccaat ctcattgatc tctggtgaat
     2581 ttgctggaat gtttccccaa aaaggtggtc cagaactttg ggtatcgaat gctctaggaa
     2641 aaaaatgggg gttcgttgtc tcatggctgt tatgggtcca aatgtttcct gggatggtga
     2701 tggtcgcttc agcactcgct ccgttgtttg gtaatattat cgataatgtg ccgcttggtt
     2761 tgaacagcaa atttacatta gttgtcattt tagtcgttta ttggatcatc acttttttga
     2821 atttgaaatt tgatatggca aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg
     2881 tgcctttgac gatgatgctc ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac
     2941 caaccggaac attaggcagt ttttcatggg acaaattaat accagatact acgactgcat
     3001 cctcatttgt ttattttgcg ccgatcatgt ttatttttac tggtatcgaa atgtcctctg
     3061 tgtatatcac tcgattggaa aatccagtta aaacgtatat tcgcggagta tttgccgcgt
     3121 taatttttat atttttcgtc aatattttga atgctttagt tgtagcaaat gtcgtaccaa
     3181 aaggacaaat ggaattgaat aatatcgcgc aatctatttc gatttattgc caaattcttg
     3241 gtttgccaca tcttattgtg aatctgttta gtttacttgt cttcattggt gtagcagtcc
     3301 aattatctgc ttgggcctca ggaccagcaa agacagtgac agaaagtgct agaaaaggtg
     3361 cgtatccacc aaaattcaat ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta
     3421 ttttgactca gtctgtcatt atctcaatct ttgcactttt ttatttgctg attcctggcg
     3481 tcaatcaagc atttttgatg ctcgtaaatt caactactgt tatctactgt atcgtctatg
     3541 tcatcatggg gatcgcagtt ttgagattgc gttatacaca tgcaaagttg aatcgaccat
     3601 ttagaattgg aaaaaaagag aaaaagagca atcttggagt ttggactgtc gttattgttt
     3661 tatttgcggc aatcggattt tctgtcggac tgacgatgaa agctggaaca tggatcaatc
     3721 taattgcagt cacagccatc agtgtcattt tgtttgtagt accactctgt attgaaaaaa
     3781 taaagaaacc ttcttgggaa caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac
     3841 ttgatggaaa aaccggcaat gaaacggact ctaactaatc gaaatatcca gttaatcgca
     3901 ttaggtggcg caattggaac aggattattt ttaggttcag caaaggcaat tgcatcagca
     3961 gggccagctg ttattctcgt ttatttgata tgcggtttgg ctatttatgg tttgatgcgg
     4021 gccatgggtg atttattttt agcaaatagt acatttaact ctatcagtga tttcgttgac
     4081 tattatttag gcaaaaagtg ggcatttttt gtcaattgga cttattggct ttgctggctt
     4141 ggagctggac ttgcggaatt aacagcaata ggattgtata tccatttctg gtttgtacat
     4201 gtaccagctt tgataagcgg aggtagcgca ctaatgatct taatggcagt caaccttgtt
     4261 gcagtcaagt ggtttggtga attggaatct tttttctcaa tcatcaagat cttggggatt
     4321 ttccttttta tcttattggg tataggattg agtgtctcag attttcatac ccagtctatc
     4381 ggaatcaaag aaaattttgg acagatcgat gaattactac cgtttggctt tgctggaatg
     4441 gtagtctcat ttcctatggt tttattcagt tttgctggaa ttgaaatgat cggcttaacc
     4501 gttggcgaga caagtgatcc gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg
     4561 cgtattctct ttttctatat aggaacgatt ttgtcgattt tatttgttgt tccatggcat
     4621 tgtttagatt taaaagaaag tcctttagtg actatgctgc aagtgatcca ttgccaaggc
     4681 ggagcagcaa ttgtcaatct aatgattttg gttgcttctt tgtcctcagc aaatagtgca
     4741 gtctacagta ctagcaggat tttatatcaa acagcaaaag aaaaaagttt gccccattgg
     4801 ttttgtgcgc tttctaagaa aggcgtacct ttacacggta ttttaattac aggaatgtta
     4861 ttttttgccg gtctttgtct acattttttt gtagctgata gtcgattgtt ttttcagctt
     4921 ttggcaggca tgacaacgag ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat
     4981 gttcaattta ttaaagaaaa gaaacggaac actacacaaa gatataaaga tcttgctttg
     5041 ttggtgtttt ttacttcaat tgctttcagc ttgttattcg aaagcacgac acgctttatt
     5101 ccttttttat tggttatctg gtttgtattc ttaaattttg tatataaaag aataagcaat
     5161 agaaatatca gttaggccca gatacatttt gcaaaacgaa ttatttcaga cccccctgaa
     5221 ataatcgata cgcctgaaaa aacttccctg cgtataataa cagtatcaat taaacaggag
     5281 gggataggat gaactggtac agattgacgg tagaagatgt attgaaaaac tatcaaacat
     5341 caacagaagg cttgtcgcaa gaagaacgac agaaacgcct gactgatcaa ggatataatg
     5401 aaataaaagc aaggcagcag acaaaacgat ggaaaaaaat agcaaagcat tttactgatc
     5461 tgctgatgat cgtgttgatc attgcctctt ttttgaaatt tgcttctagt gaatatattg
     5521 aaggaagtat tattttattc gtagtgatcg tcaatggact cgttagttac tggcaagaaa
     5581 gaaaagcgga agaatcattg aatggtttga agcagctgat gggacaagaa gcgatagtac
     5641 tatcaaacgg gatgcaagaa aaaatccctg cgaaagaact ggtaaaagga gatgtcattg
     5701 tcttacgtgc cggagatgtc gttccagcag atatccgatt aatcgaagca catgatttat
     5761 tagtggaaga atcgattttg acgggagaat cagaagcatc tgaaaaaagt catgccgttt
     5821 tgacagaaga agaatcgatc ggcgaccaga aaaatatggg tttttcagga acgcttgtac
     5881 aatctggttc tgcagtcggt gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa
     5941 tcaatcaagc attgcaatcg gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc
     6001 gattaaacaa acaaattttt caaggcattc tttgtttgag cctattctta gttattttta
     6061 cgacatttcg atatggtatg gattggcata tccttctttc ggctacgatt gctttggtgg
     6121 tgtcaatggt acccgaagga ttgccagctg ttttgaccat gattctatcc gttggagtac
     6181 atgaaatggc aaaagaaaag gcgatcatca aaggacttcc ttcggtagaa acattaggtt
     6241 ctatgacagt gatctgttca gataagacag gaacattgac aaaaaatgaa atgacagttg
     6301 tggatgtagc tgcaaaagaa gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat
     6361 taaaaacaaa agaagaacag aaaacagaaa atttaagtgg caatccgaca gaagttgcgc
     6421 tgattcgaca cacagaaaca gccaatctac ctttgaaaaa aatagaagca aagattccgt
     6481 tcagttctga atacaaatat atggcaacga tgcatgcgga ggaaaaagga gcggtgattt
     6541 acgtcaaagg tgcaccagaa gtattatttg ctaaatcaac tctttcttct gcagaacaag
     6601 aagcatggag tcagacagct gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg
     6661 cttataaaaa agtagattcg aaacaggaac tcacgcatga gactttaacc caattaactt
     6721 ttgcagggat cgccggattg atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg
     6781 aatgccaaca agcagggatc tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa
     6841 aagcgattgc ggaacaaatt ggcttaaaac atactgccaa agttttagaa gggatcgatt
     6901 tggatcttat gtctgatgaa gagttgattc agcaagtgcc aatagtcgat gtctttgctc
     6961 gaacgacacc agaacataaa ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg
     7021 tcggaatgac cggagacggc gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa
     7081 ttgctatggg aatcaaaggt agtgaagtaa gcaaacaggc agctgatatg gttttaggag
     7141 atgataattt ccatacgatt gcaaaagccg taaaagaggg ccggcggatt ttggataatt
     7201 tgcaaaaaac catcaatttc ttcttgccaa ctgcattagc tcaaggattg atcttgattt
     7261 gggcacttat gctgaatcgc ccgctacctt tgtccccagt acagatttta tgggtcaata
     7321 tggtaacgac aatcacttta tcttatgcac tagggttcga gtcgccagat ccagaaatca
     7381 tgaagcgccc accaagagat ccaaagcaag gaatcctatc tggttatcat ttgtttcgga
     7441 tcatctatgt ctccctactg atcatggttc ctgcctattt attggctatg cagtttgaag
     7501 ggcaaagctt acagcaaacc attcttctgc aaaatatcgt cttagctcaa gctgtttaca
     7561 tgatcaattg cagagagttg tccaagccgt cattgaacca aggtcttttg caaaacaaag
     7621 cgctttttct ttcattaggt atccttgctt tattgcaagg gatggttatt tttctaccag
     7681 tcggccagca gctgatcggt acagtaagtt taacgatgac acaacagtta ttgattggag
     7741 caaatgtgat tcttctattc ttagttgtag aaatagaaaa agggatcatg aacaaactgt
     7801 ttcgcaaaaa agaaaaaaca gctagagaat acagtcgcta gccgttacta taaaaaagtt
     7861 ttgaaagtga gttcagtcaa acgattgagc tcatttttcg ttggaataga agaaatgtat
     7921 acaataggga tatctagaga atctagaggg aaattggaaa tcaagatatc tgttttttca
     7981 aaagaaatag tggaaagatc ggttagttcg ataggtaaga agcagacacg ttttcctaag
     8041 taatctttta attcctgctg caagaacgcc ttccatgaag gttctccttg gaaaataaaa
     8101 tagacattga acaaattcgg tttgatagaa aaaatcgttt gttggatcag tagagacaag
     8161 agttggagaa agtaatttgg ttcattgatt tctatttctt tttttagatc gttcagtaaa
     8221 ttctctttct gttgatacaa gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg
     8281 tattgacggt ccaatgcttc aatcaatatc ggcgattcat gatattttaa tatgaggaga
     8341 gccaagttat cagtcagaga tgtttttttt agttgatcaa gagacagctc tgaagctagt
     8401 tcctctacaa acgtttcgag gacctctaaa aatggagaat gatgcaggca gcgagcggtt
     8461 tcttgtccat agttattgtt gtaattccaa ctttccagaa agcagaaaga agcaaaagaa
     8521 agctcagtgt ctttaagata aataccttcc gttaaataca attttttcaa tgcagagtca
     8581 tacagggagg agagtgtgct gttggtatgg atagaagaat ctaatcggca attcgctttt
     8641 atacggatcg tattgataaa gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct
     8701 agttcagttt gactgaggtt tcttaaaaat tgaaaataat gggattcatg gatcgaataa
     8761 tcctcgaaaa aatagttgtt atgagtgaaa ggaatcagca atcgatgata aaaagtacga
     8821 atcgcacgct cttttccaag aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg
     8881 taacttgcta aacgctgatt gattttagtc acatgtcttt tcaatgtccc ataagaaagc
     8941 ccatggtcgg ctagaaaatc agggacagca actttttttg taaaaagcag atttttcagc
     9001 agttcgacac tgactgattg tttgaaaaaa atctcccaaa ggtcatttgt taataaattt
     9061 tgctggtttt gcaaactgat tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc
     9121 tgagagcgaa tagattgaag gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca
     9181 gctaattcat ttacggttat ctttgtatga ttcagcaatt gttctactaa agaaacatga
     9241 cgtcgaatat ctttttcggt aatgattttt ttcattagtg aatacatcaa tcagactccc
     9301 ttctattcta agaatacgga gaaaataaac ggtggtcaag tttcaactga tttttcaatc
     9361 attgtttatt tttctggagc atttactgat gataagaatt tcatcatgaa cttgaaaaat
     9421 gataccacta aaatcaaaaa ataggtgggc aaacaataat aaacatttta atagtagatg
     9481 gtagcaagac caacacaggc ggattgaaca tttaagagtt gataaaaaag aaaaatatcg
     9541 aagtatttga ttagcaacta cttgtattca tttttctatt cttacgtggt atctttgaat
     9601 aaagcgaatc aataagaaag agatggaagc agatgctaac gaaaaaagaa ctaatcgatc
     9661 acatcaatga aacatacaat ttgtctccag aatatatttt taaaaaattt cctgattatt
     9721 gcgtattcag acatcaaaaa agcaaaaaat ggtttggggt cctgatgtct gttccagaag
     9781 aaaaaatcta tgatgatgca agtgaccata ggatcatcaa tattcttgat ttgaaggtcg
     9841 atcctgaact tggcggaatt ttgcgaggaa aagaggggta ttttccagcc tatcacatga
     9901 ataaagaaca ttggatctct attgatctac gtaaaataga aaagctagat gaactagcag
     9961 atttgattga tggaagtttt aaatcaacgc aatgaatgtc taggacaata gagaagaggt
    10021 tgtgacagaa atgtttagct tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa
    10081 tttttgggaa atttcggctt attttctaag aaattacttc tggaacaccg tttgttcgaa
    10141 aaaagagctg cgacaagaat tttgtcccag ctcttttttt tttattttcc taaataaaat
    10201 tcccgcatcg tttttgcaat cattttgatc aaaggatgat cttctgtgaa gctagaagat
    10261 aaataaattg ttcgtgtcaa gggcaaatct ttgatttccc gcaagacgaa aggatcattt
    10321 ttatcgtaag accaagaaac ttttgggaaa aagctaacag ctaatccttg cttgacaata
    10381 gaacgtaatg tcgcaggatc atctgtcgtc gaactgatcg tcaattcgat acttttcgct
    10441 tcaagtgctg catccagtgt atgacggaag gcgttgctct ttgacagcat caagatgggg
    10501 gtgcgttgga tatcttctaa ttgcagatgt tcttttttag caagaggatg tttcttagga
    10561 atggcgacat aaacaggttc tttcatgata ggaatattgg tcatgccagg cttcatctcc
    10621 gaagtgatgt acagtacagg atcggtaata tcatctggta aatgatgttg catgatcgtt
    10681 aattggatat caggatgctt ttccataaaa attcggacca agttcggaat agaaacagag
    10741 gctacctcta aatacaatat gacggaagta acgatcttct cattgaccga agacaataat
    10801 ttgattcctt cttctaatgt gtctaatgtc ttgactgcat atggataaaa ttcttctgca
    10861 taaacggtcg tctcaatgtt tctgccgcta tggtaaaaca aagggacacc taattcgttt
    10921 tccaaatgtt tgatcgcacg gctcaaggca ggttgagaaa taaataattc tttggctgct
    10981 tgggtaacat tttttgtttc gaatactttt ttaaaatact gcagatgaag caatttcata
    11041 attatccatc ccatccatac ctttttgtta tggttttttc tttttttggc attttttatt
    11101 atagcagaaa ttgactaaga tagcagtgga aaaagtaagt gctgaggagg cgaggctcat
    11161 gagtacaggt ttcttatttc ctggacaagg cagtcagaag ttagggatgt tagcggatat
    11221 ccctgaaatg tacatggaac gtttagaaaa tgtgacgggg tatcggttag ttgataaaga
    11281 agaaaactat caagatacgg tttttatcca gctagcatta ctgacaaaag cagtatttta
    11341 tttagatgaa atggaaaaaa acggtattca gcctgatatt gcagcaggac attcgattgg
    11401 tgctttttgt gcagctgttg cttgtcgatc tttgagattt gagaatgcag cggaactagt
    11461 ctatcaccgc gcagtcttaa tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt
    11521 agtaggtgtg acgagaagcg aagctgagga aattgtagca aaaacatttg atcctgacca
    11581 tccagtttat ctgtcaaatg aaaattgtcc gatgcaacat acgttatctg ggagtatcga
    11641 aggtttatcc gttacgttgg cagaagccaa acggcaccaa gcgcaaacag cgaagctgct
    11701 gaaagtccct gtgccatctc actgtctctt gatgaatgat acggtcaaga aattcaagcc
    11761 gtatatgcag tctgtccatc tatctgatgc tagatgtttg tatataaaaa atacggatgg
    11821 aagagccact caggacgcag aagagattcg taaagatttg ttggaaaacc tagctttccc
    11881 tgtgcagtgg aaccaaatga tggacttggc aaaagaatta ggaatggatc tgacgattga
    11941 atttcccccc ggcaatactt tgacaaagct gattcataca aaatttggtg tagataaagg
    12001 gatccgtacg atcaatctag accaacatgg tattgatgac gccacgtttc tatatcaaaa
    12061 atggagatga ctaattatgg aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa
    12121 agcgcagcga ttggaaaaaa tcagcaactt gctcaatggc aagtatgtag aaactgaaaa
    12181 aatcgtagaa gtaatggaaa aactgattgc tcccggcgac aaagttgtat tagaaggcga
    12241 caatcaaaaa caagcaagtt ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt
    12301 gaatgatctg catttgatca tgtctagtat ctcacgacca gaacatttga atatttttga
    12361 aaaaggaatc gcaagcaaga tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc
    12421 ccaaatgatt gaagacggca caatgaaatt aggtgatatc catacttatt tggaacttta
    12481 tggacgctta ttcatcgatt tgattccaaa tgttgtttta gtagctgcag ataaagcaga
    12541 taaagacggc aacttgtaca caggtttcaa tacggaagaa acaccaacaa tcatcgaagc
    12601 tgcagctttc aaagatggta tcgtgatcgt tcaagttaac gaagttgtcg attctttacc
    12661 acgtgtagat atccctggtg actgggtaga cgttgtcgtg aaagcagata aaccttatca
    12721 attagaagct ttattcacac gagatcctca aaacatcaca gaattgcaaa ttctaatggc
    12781 aatgatggca atcaaaggaa tctacgcaga acacggcgta caatctttga atcacggtat
    12841 tggattcaat acagcagcta tcgaattgtt actgccaact tatggtgaat cactagggtt
    12901 aaaagggaaa atcgcgaaga actgggtatt gaacccacat ccaacgatga ttccagccat
    12961 cgaatcaggt tgggttgaaa gcatccatag ttttggtggc gaagttggta tggaaaaata
    13021 tattgctgct cgcccagatg tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg
    13081 aacattatca caagctgcag gacaatatgc catcgatatg tttatcggtt caaccttgca
    13141 attagattac gaaggaaact cttctaccgt aacaaaagga cgtttatctg gttttggcgg
    13201 tgcgcctaac atgggacata atcctggcgg tcgtcgtcat tctacaccag catggcaatc
    13261 acttcgcgac aatgatgatc cattagggaa aggccaaaaa ttagttgttc aaatggttga
    13321 aacatatggt tcaaataaaa aaccagtctt tgtcgaagaa ttagatgcac tagctgttca
    13381 aaaacaagca ggcttagcca atgcaccagt gatgatttat agcgaagata ctacacatat
    13441 cgtgacagaa gaaggaattg cttatttgta caaagcaaaa acaaaagaag aacgacaagc
    13501 agcaattgca gcaattggcg gtgttacacc tcttggaatg actagcacga aagaatcact
    13561 agatagcttg cgtgcagccg gcatcgtgaa attaccagaa gatctaggca tcaaacgtag
    13621 tgaagcgaaa cgttcattat tagctgcaca aaccatcgat gatttgatcg aatggtcaga
    13681 tggcctgtat gaaccaccaa tgaaattcag aacttggtca taaaattggc gagaaaaacc
    13741 ttttgtcgcc atttacccag atggaagaag aaagacgaat aagcagaacg tgtatgctct
    13801 gttttacaaa aaggggagag atccatggaa caattagctt ataaatatcc aacgaaacag
    13861 ccgatcaata agaaaatcca tgtaggagtg gtcggttcag gagatttgga aatcttgatg
    13921 tttccaacag aaaaaacaga atctactgta aaaatttgta caggaagcga tggcttcggc
    13981 gaagtatgga acaatgtatt aacgagattt tttgatcgtt atccaatttc agcagatatc
    14041 gtgatcaatg acttcggtgc tacaccagga gttgtgtacc tacgattgac gcaagcaatg
    14101 gaggaacttt cagatgaaaa atagttttgt agaattaaac gccagagaac gagtgaacgc
    14161 tctacttgac aatggacgtg aattgattga tccattcgat caaatcatcg cgccaagttt
    14221 accagcacaa ggaatcgtcc ctgaaagtga tgacgggatc gttgtcagcc gtggcacgat
    14281 caatggcaaa caagcagtag taatcgctat ggaaggaaaa ttccaaggtg gcggtatcgg
    14341 tgaagtttcc ggtgcaaaaa tcgttgctgc acttgaacat gtgttagaag aaaataaaaa
    14401 aggaaatgag atctttccag tcattgtact agatacaggt ggtgttcgtt tgcaagaagc
    14461 aaactacgga ctgttatcta tttcagaaat cggaaatgta atcattgctc taaaacaata
    14521 tgtaccagtc atcggtttga ttccaggacg tgtaggatca tttggcggta tgtcgattac
    14581 ttcagcatta atgagttatt taatcgcaac aaaaaaagcg agagtcggtt tgaatggacc
    14641 agaagttatt gaacaagaag caggcgttcg agaatttgat tctagtgaca aagacttgat
    14701 ttggaataca ttaggatctc gtcaacgggt agaatcaaaa atcattgatg aattagtgac
    14761 tgatagtgta gaagcaatca aagctgctgt catcgcagca atggacgaac gaaaagacac
    14821 gcatcgcagt gaaagctcag cattttattt atcattactt gattctttag atctttcaaa
    14881 accaatggcc attgacgatt acaatgaagc aattgctaac catcaagcaa aatcaattga
    14941 cttaccagaa gtagcagaag gaaatgaacc agctgcagca agtgttggtt acgattggtt
    15001 tgcggcacta actggcatga aaaatccaac ttcttctatt agtactgtgt attatggtaa
    15061 atcaagtaaa ttcatagaag aagccatcgt gacatcaatc gtacctgatc ctaaaaaccc
    15121 gatgtaccgt gtacgaaacg gtgaagttgg tttagttgaa ggtttccgca tgggacaaat
    15181 tttggatcac gtttacgaag aagataaaga caaaaaagtg aaacggccaa tcatcgttgt
    15241 gatcgatgta ccaagccaag catacggcta taacgaagaa ttgatcggta tccatgtggc
    15301 tttggctaat agtgctgctg cttatgcaaa actccgtcaa gctggacatc cagttattgg
    15361 attgattgca ggaaatgcta tttcaggtgc atttttagca catggattac aatcaagccg
    15421 cttgattgct ttaaacaata aagaaattac cgtccaagcg atgtctaaag aaagtgctgc
    15481 acgtgtgaca aaacgttcgg ttgctgaaat cgaagcagct gctgaaaaag ttccagctat
    15541 cgcttacgat atcaataatt atacaaaact aggagcgatc tatcatttcc tagaagatat
    15601 caccgaccaa gctgcttcag aacaaaatgt cagcaaagtc atcgaagcaa tcaatgaagc
    15661 cattcaagat gtccgcaata caaatgacac gatgctgaca aatcgataca caaacgaaac
    15721 agccaaaaca ttcggacgtg tcgaaacaaa taaagtccgt gccaaaatgg acgaagaatg
    15781 ggatgcataa aaaatgattc aagcacatga tattgtcctt tttgataaac atgaaataga
    15841 agggttcacc ttgcctgaat gggtcgttct aactgaaaag atgttcggaa cagttcgtcg
    15901 tgcgtcgacg gatcacgcag aaatactacc gattggttta cgtggacctg cccgcaatca
    15961 gagatggtca acagaaatct caaaacatgc cgtcaaacaa gtcattcatc cgtgggacat
    16021 catcgaacaa gaatcgtata gacaagagaa aatcatggat tatcctgttt atcagcaatt
    16081 tcatgctgct agaaacatat tatcaggatg taaatgggga atcggcggca gcttaggatt
    16141 tgaattatcg actgggattc cggcagtaaa agaaaccagt gattttgatt tgctgctgta
    16201 cgcaaattca ccgattgaat tgccaattca ggtaattcaa tcgcatcctg ctttttttga
    16261 gcaattcgat acccaagtag tgactcggca aggcggtttt tcactaaaag agtatttgag
    16321 agagccagag aaaaaaattc tattgaaaac agtcgaagga cctaaattaa ccaaagaact
    16381 ttggtaataa agaaaaagag agagaaagag atgtttgaaa acaaaaaaat caaaatcaac
    16441 gtaagtgact tccagaaagt atcagctgta acggctgtta tgatgcaaac gatattgagc
    16501 tttgctttgg caaacagcca cagcgcaaca aatacagctt ttcttggtac cttttacgtc
    16561 atcgttaaat acacagcacc aatgtttatt tttgcaattg tttataatat ggtaaaaaca
    16621 agtcagcatt tgtcttattg ggaatttttg aaagaaaagt tttttgaact agtcgttccg
    16681 tatcttctat ggacgacggc ttatctttgg ctatttccag cagtccaaca acaaactccg
    16741 tatacggata ctgcaagctt tttactgaaa tatgtcacag gtgatggagc gcctcattta
    16801 tggtataccg ttatgatgct gcagatccaa ttgttcatgc cgttttttgt ttggctcggt
    16861 tacaaagtat ttgctaataa aaaatatgta tggccagtat tgattggttc aacgatttta
    16921 tatgtcgcat ggtatctgtt ctatcaaaca caagtattga ctgggcctta tcatgacagc
    16981 tggtacttat tagatcggtt tgttgctagt ttcatgattt atggggtagc cgcatttgtt
    17041 tatcacgaaa aagtctatca atatttggac agagtacgtt atcttttctt acctgttgcc
    17101 ttagttatcg cgttcttttc tgtccgttca ttgctcgcac atccaggaga cttgagcttt
    17161 gcgaatgcac cttacttgaa tacgatccaa agtttataca gtttagtcat catctttgct
    17221 gtcttcatag gcgcttccaa aatgattgta aacgattcgc caaaattgcc attgttcaaa
    17281 tggttgtctg tttatgctta tcgtacgtat ttagccaatg tatttgtttt ccaagtactg
    17341 ttattattgt ttaaagatag ttggctgcag ttaccttcag gagtcatgat tcttgtagct
    17401 tatctaatga cggcaagttg tgcatttgct ttaagctggt tacttcatat catttgggta
    17461 gctatcaaaa aaggtttcag caagtaatag taggaagaag gagtgagtgt catgataaat
    17521 gacgaacgaa aaaaagcagt gactcagcaa ctcgctgcgt tggtagagga agcattgatc
    17581 gcagaagtaa cattaagtcc aaaacctgga ttagtggatg cactggatgc gggtgcacat
    17641 tccgatatgg actatgcttt atttcttaag tctgctaaga cgctaactcc tttttttgaa
    17701 gaaatggctc aaatagcatg gcatcatgct attgatcagg agttaagaga aagaattgct
    17761 gagattgggc gaaaagcgga aaaggcaatg ctgattgcta caaatggcat caatacccat
    17821 aaaggagcga tttgggcaat ggggctattg atcagtgtca ttgcacaaaa aatcagtatg
    17881 gaacagcctt tttctttatc gaaaatactt gatgatgtca gcaagcttgc tgcttttcct
    17941 gatacaaaat atcgtcataa aaaaaccact catggtcata gggtgaagca aaaatatggc
    18001 gtaaatggtg catatgaaga agcgatatta gggtatccgc atatcaggct tgcaattcaa
    18061 gcagctaagg agctagataa tgattcatat gtacagaaac aaatgcatat gttgttagtt
    18121 ttgatgagtt cgctagatga cacatgcgta ttgcatcgca gtgatcagaa aacgttagtg
    18181 gaaatgcaaa aacttgctca tgctgctagt caaagtgctt taccaaatcg tccatttaca
    18241 gcactgttgc acttttgtca ggaaaaccaa atctctcctg gaggaagcgc agatctcttg
    18301 tctgcaagct tgttcatgct gaagatagag gaattatgga ctgccgacac atacatttgt
    18361 ccgatgttac tcaatgacta aataagacaa aattgctaaa aaaacagttt atgcatctgg
    18421 ttattgactc ttccttttag caaggctatc ataaaaatag ttttttgtaa taaaagataa
    18481 cgaggaagac aatgaaaaaa atcaattatt tagcaatcat tttgttagtg ggcctatgtt
    18541 tgtttgctct gccgacccaa ggattcgctg tcgcgggtgg tcatggtgga ggtacatcta
    18601 gcggtcgaag ttcgggcaga ggttcatttg gtggtcgttc atatgggggc agttattttg
    18661 gcagtcgttc ttacggcaga gattcttatg ggtattatgg tccaggtaca ggtccttcta
    18721 gttatgagca aacgggattt cttgcattag ctgttttgtc agtaggaggg gtttctgctt
    18781 tttctatcgt caagaataaa agacaaaaga aaaggctgaa agcggagata ttgtcccgca
    18841 tgtcaggaac ggctaaagaa aagaaagcac agtttctgga aatccaaaag atattcatga
    18901 agatccaatc cgcttgggaa gaagaaactc ctgaaaaagc cagaagctgt tatacagaca
    18961 agctgtttgc cgaacatcag aaagttctgc aaaaaaacaa agcagacggt gtaagaaatc
    19021 atactaaaaa aataactatt caggaagtag gaaattatcg acagatccac gaaaatagtt
    19081 ttgccatccg aattgatttc agatgctgtg attattcagt cgatcgaaaa gatgggcgat
    19141 tgatctcagg aagtaagaca caacgacagt atttttctca gatatggtat tttgattttt
    19201 ctgagaaaga aaaaagatgg caagccgatt ttattcagcc gattatgctg gattgacagg
    19261 aaatatttat gaacggagtt gtgacaaaag ccttgtcgca gctccttttt ctgaataaac
    19321 ggtgtgccag agatagccta tcggcaataa ggcacgattt tgtaaaatat gggaagcgat
    19381 ttttcaaaat cgcttcttat cgctcaaggc tgaacatctc tgtcacaacc tctccactaa
    19441 atggtgttta aaagcttgca tctgcgataa taagaacaac caaacgaaaa ataggaagca
    19501 ctattttagt ttagttgttc ttattacttg atgcaggaca gctttttcac aacctctttt
    19561 ttgtatagat gggcaaaatt tttgtaataa taggcgtatg tctttttcgt tgaggagatg
    19621 aaaaagcata tggcttattt tttgtttgct ttgcttactt tctttccact tcaagtggca
    19681 gaaatatcta ctttttatta tgtaagcgga accatatact tgaaatcaga aggagatcat
    19741 ttctatgaaa acaagaacaa ttgaaatact tcacagaatc atgacagccc aatatccagt
    19801 aaaagcaaaa catctagcaa gtgagttcaa tgtcagtcag cggacgatcc gtcaggaagt
    19861 tcttgaagcg aatatctggt tgagcagtaa aaagcttccg gagatccgta caattcgcaa
    19921 caaaggtttt ttgttgaagc tggaaaagga aaagcaagct cgattagaaa ctgaaataaa
    19981 agatgtgaaa gacggctttt tgaatcggcg cgaacgtatt tttgatctag ttttagccgt
    20041 tgcttacagc cgaatcccga tccatctaaa caaaaaagaa gaagaatttc aaatctctaa
    20101 aagtacatta gatgaagata tgagacggtt acgagcaaca ctggtcagtt atgggataga
    20161 gatcgtcagt tatggaaagc aaggactggt ttataaaggt tcagaacgat ctatccgtac
    20221 gatgatctat gattttatca atcagaatct gggaatgacc gacttcttta tgaatcaaga
    20281 tgcacaatta acaccggctc aaaaaatctt tctccattat tttcctttgg tggaaatcaa
    20341 aaaaatacag gagatctatt ccaataagtt gttgaaacaa gaagatgata tttataaaaa
    20401 acaaatcctg ctttttacat tgatctggat ccagcgaatc aggaaacatg aatttatctc
    20461 tgccattaat tggaaaaata cagaaataaa agacagtagc ttttctgcat ttattgacca
    20521 catgatcgag gaatttcaat tggacaatgt cccgcaagta gaaagaaatt atcttcgttt
    20581 cactattgag acgttcaatg ctagagatat ctccaattca atcgagtggg tacaagctca
    20641 attactaacg atccaattga tccaatttgt agagaacgag acacacgtac ctttccatct
    20701 aaaggaagaa gctttattcg aaaatctcta taagcacatg gctgcactga ttgtaagaat
    20761 caagaatcat atccaagtca tgaatccatt gaaagaaaac atccgaacaa attatttccc
    20821 aatctaccgc gcagtcactc attttgttcc tacgatcgaa gaagtaattg gtggcaagat
    20881 actggaggac gagatagctt ttcttgtcat ccacttttcg acgatcgcca gtgtaatcaa
    20941 gcgagacttg aattatgttt ataaatcagt cgttgtctgt aaccatggga tggctactgg
    21001 aaatctgtta gcagagaatc tgaaagaaaa gtttcctcag atcgagatta ctgcagtttt
    21061 aagttcaaaa gaaatcgaat tagtagataa attggatgtc aatctggttt tcagcacctt
    21121 tcaattgaat tatcgaaata agccgctttt agttgtcgat ccgattttaa ctgagacgaa
    21181 tatcttggtt atagaagact ttttgaaaag aaatgaagaa ctacagcggt tagagcctaa
    21241 cagtcatgaa tcgacggagt tattcactca aataatagat gtcatgaaag aaagtggtgg
    21301 ccgagtagat cgaatcatct atggcaagtt agaaaaatta ttcgaaacaa acaatttaga
    21361 aatcaacaaa agggagatcc agcctatgtt aaaagatatt ttgacggaca gcaatatcat
    21421 gatcaaagaa aaagccacta cttgggagga atcgattgaa ctagctgcta gaccattggt
    21481 taaagaaaac attgtcgaag agcgctatgt cgaagcgatg attcgtgcag tgaatgatta
    21541 cggaccgtat atcgtgattg gcaaacacat ggcactagcc catgcccgac cagaagatgg
    21601 ggtcaataaa ttaggagtca gcgtagctac gattgagcaa ccaatcgatt tcggtaatcc
    21661 tgaaatggat ccagtaaaga ttattttttg tttggcagca gtagactcct actcgcatct
    21721 gaacatcatg aaagaactaa ttgaattgat caacaacgaa gagaagttgg atcgattgat
    21781 tgaatgcaca aatatcgaag atttcaaaca actgcttttt tcagtagttg agaaataata
    21841 aatgatacgg gggagaacaa agatgaacaa attgaacatt ttattcgtat gcggtgcagg
    21901 tttaggaagt agttttgcag cacagatgtc tgctgaggat gttttgaaca aattaggagt
    21961 agaagccaaa ctggaccatt gtgatatttc ttccgcagtt tccatgaaac cagatgtgat
    22021 catcacagca gaaaattttc gttcgcagtt cgaaaagttc gcagttgatc ctaaaacggc
    22081 gatgatctat ttgaaaaata ttgtttcaaa aaatgaaatt gaagaaaaaa tcaccccagt
    22141 tctgcaagat aagggagtta tctaaataca aaaagaaaag agggtaaaaa
//
LOCUS       DAZKPO010000056        22124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000056 DAZKPO010000000
VERSION     DAZKPO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.056"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACG8NZ_001926"
     CDS             complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACG8NZ_001926"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0539588.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACG8NZ_001927"
     CDS             complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACG8NZ_001927"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0539589.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACG8NZ_001928"
     CDS             complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACG8NZ_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0539590.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACG8NZ_001929"
     CDS             complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACG8NZ_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0539591.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACG8NZ_001930"
     CDS             complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACG8NZ_001930"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0539592.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACG8NZ_001931"
     CDS             complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACG8NZ_001931"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0539593.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8344..8814)
                     /locus_tag="ACG8NZ_001932"
     CDS             complement(8344..8814)
                     /locus_tag="ACG8NZ_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0539594.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8860..9285)
                     /locus_tag="ACG8NZ_001933"
     CDS             complement(8860..9285)
                     /locus_tag="ACG8NZ_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0539595.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9352..9621)
                     /locus_tag="ACG8NZ_001934"
     CDS             complement(9352..9621)
                     /locus_tag="ACG8NZ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0539596.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9632..11227)
                     /locus_tag="ACG8NZ_001935"
     CDS             complement(9632..11227)
                     /locus_tag="ACG8NZ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0539597.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACG8NZ_001936"
     CDS             complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACG8NZ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0539598.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACG8NZ_001937"
     CDS             complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACG8NZ_001937"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0539599.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15693..16667
                     /locus_tag="ACG8NZ_001938"
     CDS             15693..16667
                     /locus_tag="ACG8NZ_001938"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0539600.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16837..18237
                     /locus_tag="ACG8NZ_001939"
     CDS             16837..18237
                     /locus_tag="ACG8NZ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0539601.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18315..19037)
                     /locus_tag="ACG8NZ_001940"
     CDS             complement(18315..19037)
                     /locus_tag="ACG8NZ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539602.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19116..19622)
                     /locus_tag="ACG8NZ_001941"
     CDS             complement(19116..19622)
                     /locus_tag="ACG8NZ_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0539603.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19745..21544
                     /locus_tag="ACG8NZ_001942"
     CDS             19745..21544
                     /locus_tag="ACG8NZ_001942"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0539604.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21612..>22124)
                     /locus_tag="ACG8NZ_001943"
     CDS             complement(21612..>22124)
                     /locus_tag="ACG8NZ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0539605.1"
                     /translation="TTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPV
                     TPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGW
                     TVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQ
                     WFAPDFAVSM"
BASE COUNT         6126 a         4837 c         3999 g         7162 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt
       61 ttgaataaat attttcttct gtcttttgga caagtaataa tataccaact aaatttagct
      121 tggtcaacga attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt
      181 ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt
      241 tgaactttcg ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta
      301 gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg
      361 catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca
      421 taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta
      481 gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt
      541 ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc
      601 gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa
      661 acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc
      721 cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt
      781 ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat
      841 ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg
      901 ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat
      961 tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat
     1021 caattccttc attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa
     1081 ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat
     1141 tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg
     1201 tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc
     1261 ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc
     1321 tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt
     1381 ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga
     1441 tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct
     1501 catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat
     1561 gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg
     1621 tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt
     1681 ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt
     1741 gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt
     1801 gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg
     1861 aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag
     1921 aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag
     1981 gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg
     2041 aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc
     2101 tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg
     2161 tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt gaatcatcca
     2221 tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat
     2281 ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa tcgtcaaatg
     2341 ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt
     2401 acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac
     2461 agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac
     2521 tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc
     2581 tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat ctattatatc
     2641 ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc
     2701 aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt
     2761 tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc
     2821 aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac
     2881 gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc
     2941 taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca
     3001 gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc
     3061 acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac
     3121 aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc
     3181 tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc
     3241 gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc
     3301 cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg
     3361 ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt
     3421 ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt
     3481 tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg
     3541 atttttgatg taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc
     3601 tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag
     3661 tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg
     3721 acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc
     3781 gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt
     3841 tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct
     3901 tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata
     3961 aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg
     4021 acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc
     4081 tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg
     4141 tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa
     4201 agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc
     4261 gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc
     4321 agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc
     4381 ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc
     4441 ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg
     4501 acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg
     4561 ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg
     4621 tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc
     4681 ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt
     4741 gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata
     4801 agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat
     4861 cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc
     4921 agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt
     4981 ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac
     5041 tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa
     5101 acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac
     5161 gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg
     5221 ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat
     5281 ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac
     5341 acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa
     5401 cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg
     5461 tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc
     5521 tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc
     5581 agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt
     5641 catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa
     5701 aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct
     5761 tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct
     5821 caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga
     5881 acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt
     5941 gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag
     6001 ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat
     6061 gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg catttttcat
     6121 catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt
     6181 tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt
     6241 gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca
     6301 taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct
     6361 tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt
     6421 gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata
     6481 tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt
     6541 acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc
     6601 aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca
     6661 cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg
     6721 caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc
     6781 aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat
     6841 cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc
     6901 ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga
     6961 taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt
     7021 ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag
     7081 accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt
     7141 ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct
     7201 ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta
     7261 tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa
     7321 tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa
     7381 aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa
     7441 atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt
     7501 gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct
     7561 gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat
     7621 ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat
     7681 ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt
     7741 gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca
     7801 ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt
     7861 ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt
     7921 tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc
     7981 gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac
     8041 ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat
     8101 caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat
     8161 tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt
     8221 tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac
     8281 aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc
     8341 tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga
     8401 agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt
     8461 ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc
     8521 acggggatct ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg
     8581 ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt
     8641 aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga
     8701 cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa
     8761 atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa
     8821 aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact
     8881 agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac
     8941 tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc
     9001 atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat
     9061 ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa
     9121 aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct
     9181 gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc
     9241 atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa
     9301 tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc
     9361 tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt
     9421 ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt
     9481 caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat
     9541 tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt
     9601 taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca
     9661 ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa
     9721 tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag
     9781 cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca
     9841 gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc
     9901 aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc
     9961 ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag
    10021 tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct
    10081 tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa
    10141 ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga
    10201 gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga
    10261 ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag
    10321 gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac
    10381 ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca
    10441 aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa
    10501 tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga
    10561 acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa
    10621 ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt
    10681 ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac
    10741 gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga
    10801 aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga
    10861 tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga
    10921 caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt
    10981 gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac
    11041 cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa
    11101 aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg
    11161 ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat
    11221 gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac
    11281 aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg
    11341 tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc
    11401 ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat
    11461 atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg
    11521 cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc
    11581 atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc
    11641 gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc
    11701 gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc
    11761 gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc
    11821 acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga
    11881 tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat
    11941 cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc
    12001 tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt
    12061 tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg
    12121 accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc
    12181 ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt
    12241 ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc
    12301 ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc
    12361 aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct
    12421 cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg
    12481 ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct
    12541 aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt
    12601 acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc
    12661 gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa
    12721 gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc
    12781 tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg
    12841 tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc
    12901 agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata
    12961 tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat
    13021 ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg
    13081 aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat
    13141 aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac
    13201 ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc
    13261 tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct
    13321 cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc
    13381 ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat
    13441 caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc
    13501 tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca
    13561 acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata
    13621 ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa
    13681 aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg
    13741 ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc
    13801 gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga
    13861 agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc
    13921 ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa
    13981 aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat
    14041 ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac
    14101 ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga
    14161 atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat
    14221 atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac
    14281 atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg
    14341 ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc
    14401 gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct
    14461 ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg
    14521 aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc
    14581 ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc
    14641 aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc
    14701 ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc
    14761 cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt
    14821 ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat
    14881 tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc
    14941 cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga
    15001 taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc actgccagat
    15061 ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg
    15121 ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt
    15181 ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt
    15241 gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt
    15301 atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc
    15361 taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc
    15421 ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa
    15481 tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat
    15541 ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg
    15601 ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg
    15661 aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc
    15721 atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag
    15781 ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag
    15841 gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg
    15901 ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa
    15961 aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg
    16021 tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag
    16081 atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt
    16141 caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg
    16201 atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt
    16261 ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa
    16321 tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta
    16381 tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg
    16441 ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg
    16501 gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag
    16561 taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat
    16621 taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga
    16681 cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa
    16741 ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc
    16801 gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa
    16861 aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt
    16921 tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga
    16981 ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct
    17041 gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag
    17101 ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga
    17161 tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt
    17221 caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg
    17281 aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt
    17341 atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa
    17401 agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa
    17461 gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat
    17521 gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg
    17581 agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat
    17641 gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca
    17701 aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt
    17761 gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt
    17821 gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat
    17881 ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc
    17941 gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa
    18001 gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac
    18061 tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa
    18121 tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt
    18181 gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa
    18241 aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc
    18301 taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct
    18361 tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc
    18421 gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct
    18481 cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc
    18541 agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac
    18601 ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt
    18661 tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta
    18721 cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac
    18781 gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc
    18841 acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga
    18901 ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc
    18961 tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat
    19021 ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta
    19081 gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca
    19141 aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg
    19201 aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct
    19261 gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca
    19321 cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa
    19381 aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa
    19441 ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat
    19501 cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg
    19561 actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc
    19621 ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata
    19681 gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt
    19741 tttcatgagt tattcattaa attggaatct agattctatt ttttccggag gcagtcattc
    19801 tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg
    19861 cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt
    19921 gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc
    19981 tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc
    20041 tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga
    20101 ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat
    20161 tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc
    20221 tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat
    20281 tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat
    20341 gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg
    20401 gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc
    20461 tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt
    20521 gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt
    20581 taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca
    20641 agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc
    20701 agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc
    20761 tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta
    20821 ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt
    20881 taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg
    20941 ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt
    21001 cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat
    21061 caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg
    21121 attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa
    21181 gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta
    21241 ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa
    21301 cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa
    21361 gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac
    21421 ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc
    21481 aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat
    21541 ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
    21601 attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact
    21661 tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct
    21721 acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa
    21781 actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca
    21841 gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta
    21901 ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca
    21961 ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt
    22021 gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct
    22081 gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgt
//
LOCUS       DAZKPO010000057        20548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000057 DAZKPO010000000
VERSION     DAZKPO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.057"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1371
                     /locus_tag="ACG8NZ_001944"
     CDS             112..1371
                     /locus_tag="ACG8NZ_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0539606.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1460..2407)
                     /locus_tag="ACG8NZ_001945"
     CDS             complement(1460..2407)
                     /locus_tag="ACG8NZ_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0539607.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2801..4093
                     /gene="hisS"
                     /locus_tag="ACG8NZ_001946"
     CDS             2801..4093
                     /gene="hisS"
                     /locus_tag="ACG8NZ_001946"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0539608.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4108..5877
                     /gene="aspS"
                     /locus_tag="ACG8NZ_001947"
     CDS             4108..5877
                     /gene="aspS"
                     /locus_tag="ACG8NZ_001947"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0539609.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5986..6861
                     /locus_tag="ACG8NZ_001948"
     CDS             5986..6861
                     /locus_tag="ACG8NZ_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0539610.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7127..9010
                     /locus_tag="ACG8NZ_001949"
     CDS             7127..9010
                     /locus_tag="ACG8NZ_001949"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0539611.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9085..9369)
                     /locus_tag="ACG8NZ_001950"
     CDS             complement(9085..9369)
                     /locus_tag="ACG8NZ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0539612.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9359..10093)
                     /locus_tag="ACG8NZ_001951"
     CDS             complement(9359..10093)
                     /locus_tag="ACG8NZ_001951"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0539613.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10222..10845
                     /locus_tag="ACG8NZ_001952"
     CDS             10222..10845
                     /locus_tag="ACG8NZ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0539614.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            11037..12158
                     /locus_tag="ACG8NZ_001953"
     CDS             11037..12158
                     /locus_tag="ACG8NZ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0539615.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12165..15293
                     /locus_tag="ACG8NZ_001954"
     CDS             12165..15293
                     /locus_tag="ACG8NZ_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0539616.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15312..18506
                     /locus_tag="ACG8NZ_001955"
     CDS             15312..18506
                     /locus_tag="ACG8NZ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0539617.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18652..19359)
                     /locus_tag="ACG8NZ_001956"
     CDS             complement(18652..19359)
                     /locus_tag="ACG8NZ_001956"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0539618.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            20124..20522
                     /gene="spxA"
                     /locus_tag="ACG8NZ_001957"
     CDS             20124..20522
                     /gene="spxA"
                     /locus_tag="ACG8NZ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0539619.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         6894 a         3589 c         4305 g         5760 t
ORIGIN      
        1 actctaactt aagtggcttt tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa
       61 aaaagttaag cttaaattat acaagttatt ttttatagga ggttgctgaa gatgaaagaa
      121 gtaccagtag tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac
      181 atgttggagg agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat
      241 aaagaagcta tcacgatcta cggatcatca gcagcaaaag tattttatga tccaaggaat
      301 ttcaaacgaa aaggtgctat gccaaaatta gtattgaaga cactttttgg tcaaggtgga
      361 gtacagactt tggatggagc tgctcatcac catcggaaaa atatttttat ggatctaatg
      421 acaccggaaa gaatggaaga ttatcatcgt atcttagata agaatctgac acaagcactt
      481 gaagcacagc atggtcaatt tgaactattt gatttaagta agatggtttt ctttacatct
      541 atttgcgagt gggcaggtat caatttatca gcgatttcta aagatgaggt cgaaaaatta
      601 gcagaatatc aaatctctat gatcagcgga acgtttactt ctccgattga tcacataaaa
      661 ggtgtcgaaa atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga agaagctcga
      721 caaaatcctg tggctggaaa agaaaatgtg gcattatatg cttttgccaa tgccacagat
      781 ttagatggtc agcttttacc tctggaagtg gcagctgttg aattattgaa catcatccgt
      841 ccaacggttg ctctaactgt gtgggcggca ttgatgggac atgcactttt cagtagacca
      901 gatttgtatc agcaattaaa aaatgacttt tcgacgttac aagatccgtt tattcaagaa
      961 atgagacgct attatccttt cttcccaatg ctacctgcga tttcgctgaa agaagtagaa
     1021 gtagatggtt atcgtatacc agaaggcagt tgggttattt tagatttata tggaacagac
     1081 catgatgaac gaacagttga agcgccagat tcctttatga tcaaacggta tgtagggaaa
     1141 gcaaaagata tttcctataa agaagaatat gagatgattg cacaaggcgg gggaaatttt
     1201 cgacaaatgc atcgctgtgc aggagaatgg atcactcttc atagtctacg tgtgttttcc
     1261 gatcagctag taaataagtt tgagttctct gtacctgaac aagattggac gattccattc
     1321 aatcagtttc cgacttatcc gaatagccga gcgctacttt acaagaatta gattagcata
     1381 aacttatcga aaaaaaggaa gatcaaacag tgctgatctt ccttttttca tattactata
     1441 aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt tgataatctt
     1501 cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct ggacgcttag
     1561 ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa acaagttgcg
     1621 caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt
     1681 ctctgaaacg ttctccttgg aaatctggat ttctttttcg aggatctcct tcaccaaatt
     1741 gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg
     1801 gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca gcctgccgat
     1861 ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc gcttctttca
     1921 attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct tcttttaatt
     1981 cgtgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag tggatataaa
     2041 aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa
     2101 actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct tttgtagcga
     2161 tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc agttcttcgg
     2221 aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta ttcaaagctg
     2281 tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa
     2341 cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg attcttattt
     2401 ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa aagctgaact
     2461 aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact ggtttttagt
     2521 aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa actttacaag
     2581 agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac actctggaga
     2641 tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa gagaaatctt
     2701 tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat gaacaagggt
     2761 gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc aaagaccaaa
     2821 aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc
     2881 acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt ttgaacacta
     2941 tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag agatgtatga
     3001 tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag caccaatcgt
     3061 tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt
     3121 ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac gtcaattcca
     3181 tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg agagcatggt
     3241 gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag tcatcaattc
     3301 attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact atcttttacc
     3361 ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc cattacgcgt
     3421 attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat ctatcttaga
     3481 ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc tggatgcatt
     3541 ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt acacacatac
     3601 gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga tttgcgcagg
     3661 cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg gcttcggatt
     3721 tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag ttatccctgc
     3781 atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata tcgaagcatt
     3841 gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact tcatgaaccg
     3901 taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag tattggtgat
     3961 cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga
     4021 aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg atgaaatgac
     4081 tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg cggattagtt
     4141 tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa aagacgagac
     4201 cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt
     4261 aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag tgaatacgtg
     4321 atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc
     4381 actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg
     4441 ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat
     4501 ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc gacaacaaaa
     4561 gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc ttacctaggg
     4621 aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca tgctggacat
     4681 ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa tgctggattc
     4741 gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag
     4801 cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga aatccaaact
     4861 tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga agtcacattg
     4921 ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga caaaccggat
     4981 acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt agaattcaaa
     5041 gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct
     5101 gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg acaattcgga
     5161 gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa
     5221 ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc aggtgacttg
     5281 ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc tatccggaca
     5341 cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt atgggtaact
     5401 gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca
     5461 ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc aaaagtctat
     5521 gcagaagctt atgatatcgt cttgaacggt tatgaattag gcggaggttc tatccgtatt
     5581 catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa agaacaaatg
     5641 gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc acatggcggt
     5701 attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa tattcgtgaa
     5761 gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc accaagtatg
     5821 gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga agaatagccg
     5881 ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga tttaattgaa
     5941 aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa aaaaacattc
     6001 gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt ttatgcgttg
     6061 atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc aagtggtatt
     6121 actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt cgaagtaccg
     6181 gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg gttaaaaatc
     6241 gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct ttgtatccat
     6301 attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt tgggggggct
     6361 gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg cggtctagat
     6421 atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat ttcgatctac
     6481 ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata tatgttttac
     6541 agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac taaacaaaag
     6601 aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga aatccaaaac
     6661 tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa gcatgataag
     6721 cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag agaagcaatg
     6781 aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat acttggtcgt
     6841 ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac tgctgtagac
     6901 agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag aaaaactgct
     6961 tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt aaatttatgg
     7021 gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt cacttaatac
     7081 ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg aaaaacaaaa
     7141 aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat tagctgtggt
     7201 gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc
     7261 gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg aatgggaaca
     7321 aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac tctatccggc
     7381 acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc gagggctgtt
     7441 aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact ggtggcaaat
     7501 caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta cgcatatctg
     7561 gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg tattgttgat
     7621 ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat ccttcctctc
     7681 cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg tttattacgg
     7741 taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg tttggccaag
     7801 tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata tcgctggagg
     7861 aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt acaatttcat
     7921 ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg cagtgacagc
     7981 acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt ggattggcaa
     8041 acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg aagcaaaggt
     8101 gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat tgattctaat
     8161 catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg attatggtcg
     8221 tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga gcaaaccatg
     8281 ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa
     8341 aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt
     8401 agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc aagcgatcct
     8461 tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag aactaacggc
     8521 atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt atccaaaagt
     8581 agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt
     8641 aaaggaacaa gatttgttga aagacactgt tctgattgga ctaggaacga atggctcatt
     8701 ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt
     8761 caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc ttgaacagat
     8821 gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta atgaccatag
     8881 tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac attacattca
     8941 cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg agacaaacgt
     9001 gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc tgaagttttt
     9061 ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg ttgagtgtgt
     9121 ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt ctgcttttgt
     9181 tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc ttcttttttg
     9241 aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc tagaaagatc
     9301 ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa aataatgttc
     9361 atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata ttgtaagtga
     9421 tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct tcaactaaca
     9481 aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta ggtgcaattc
     9541 gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg accatagtaa
     9601 agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct aacgtcgtat
     9661 gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt gttggcagcc
     9721 ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga acgacagata
     9781 agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg ttaagcgcga
     9841 tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg atagccgcct
     9901 gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg tcaacaatct
     9961 tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa
    10021 acactttgga actttggata atcttgatat cttctgcgta taattggtcg attcgttcgt
    10081 cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat tatactattt
    10141 ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata ctatgaccat
    10201 aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt tcgagtacta
    10261 ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc gcaagatgag
    10321 aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct ggcggtagct
    10381 gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa tccagttctg
    10441 cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc aggaccaagc
    10501 gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat catgtttccc
    10561 agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc cagaatgtct
    10621 aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga tctgttcaag
    10681 cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat caagaaaacg
    10741 gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga caaattggac
    10801 aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga taagtttatg
    10861 cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa tgaaaacgag
    10921 ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga agacaggtgt
    10981 catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt gattgaatgc
    11041 gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat gacttgattg
    11101 aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa aaagtagagg
    11161 caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat gccgtagaat
    11221 tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt tttgctatat
    11281 caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc agttattcga
    11341 actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt aaaaacgtac
    11401 aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt gaagatgaca
    11461 atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag gagctatttg
    11521 attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt gtaaaatctg
    11581 attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa ctacttacac
    11641 cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga caggataatg
    11701 ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg
    11761 tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc gaacccctgc
    11821 atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc ttttatcagt
    11881 ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc atcccgaata
    11941 tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg gcaaacggtc
    12001 gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct caaaaattag
    12061 cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa gaaaaatggc
    12121 tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat
    12181 tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca cgtttgacag
    12241 aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg atttttgatg
    12301 ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga aaagagatgc
    12361 gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt gaacatcagc
    12421 agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg
    12481 gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa
    12541 taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg atgaatctcg
    12601 atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt aattttttgg
    12661 tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact caatttttcc
    12721 aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt
    12781 tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag
    12841 cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac
    12901 aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag caactagaaa
    12961 attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg atattaataa
    13021 acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa cgatggattg
    13081 ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa gaaataaaag
    13141 ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta gcgatgacta
    13201 cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa
    13261 tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct
    13321 tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa gaagaattga
    13381 atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga attcttcaag
    13441 aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga cagttaaaat
    13501 atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg
    13561 cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat
    13621 tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag cagcaaattg
    13681 ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga tcaacggaac
    13741 accccataca acaattaaaa cacgaacagc caaataagga agatatctta aaagtagaac
    13801 aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt gccaaaagtg
    13861 aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa atcatggcaa
    13921 cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa aaggcatttc
    13981 ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa tctttagata
    14041 gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga caacaagcag
    14101 aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa aataatcagc
    14161 tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat acggatagta
    14221 actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac tcgaatcaac
    14281 tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg cttcggacac
    14341 agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa aaggaacaag
    14401 aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa gaaatctgtg
    14461 catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact tcttatcatg
    14521 aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag ctaactgaga
    14581 cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt atgatcgaaa
    14641 taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg
    14701 atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt acgcaattac
    14761 agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg gaacggtatg
    14821 tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc caacgtttaa
    14881 cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga gggaaaaccg
    14941 gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg cataccctgt
    15001 ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc gatgtgatcc
    15061 agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga tttgggtcat
    15121 tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa agtgaaggaa
    15181 gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta caacaaataa
    15241 gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga taaaaaggga
    15301 gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga cgtcaatact
    15361 tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag
    15421 aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa gagatagatt
    15481 actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt gctgtggaac
    15541 tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca atgctagaac
    15601 cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac
    15661 aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa acgattgaga
    15721 ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc ggttcattgg
    15781 catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa gcttacacag
    15841 aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa cgggcgtttc
    15901 aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg catcaattat
    15961 taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg cttctcccag
    16021 tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg catctgcaga
    16081 taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct ttatcattca
    16141 aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt gattttttct
    16201 ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg aaatatcaac
    16261 aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca gaagtagtgt
    16321 tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg tcagagatcg
    16381 gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc gtcgaattta
    16441 ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc gtctatggtg
    16501 aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct tttcaggtgt
    16561 tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat cgctaccaga
    16621 aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat gcctttttcc
    16681 gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga aaattgcgag
    16741 agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag accggttcat
    16801 ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat catgtcctgc
    16861 aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt ctttcttttg
    16921 attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa tcgatacgta
    16981 cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa aatcaattgg
    17041 aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat ctcattcatc
    17101 cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga gattatcaaa
    17161 agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt attttagccg
    17221 atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg gaaaaagaag
    17281 aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac aactggcagg
    17341 cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg aataaaaaag
    17401 aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat gccagcttaa
    17461 gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt gatgaggcac
    17521 aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta gctgtacctc
    17581 agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg tggtcattgt
    17641 ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa cttgccgcag
    17701 aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa actgtgttga
    17761 aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa gaacaaaaaa
    17821 ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag
    17881 cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga caaatttgct
    17941 gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc gaacaagtaa
    18001 aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt tctcaattta
    18061 cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg acattttatt
    18121 taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt aatgctggcg
    18181 aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat ctcacgggag
    18241 cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa caagctgctg
    18301 gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg atctctgaag
    18361 gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg ttccaaaaag
    18421 tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc cgcatgattt
    18481 taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa
    18541 agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag gctgggattc
    18601 cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag gttatacaaa
    18661 gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat cgcaaattgg
    18721 agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt tagcactttt
    18781 tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt ctggaagcat
    18841 atgaaattct ttctcagcca gctgaaaaat ctctttttct ggtttttgga aacctgttgc
    18901 ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt gcttcagttt
    18961 ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat tcttttcctt
    19021 caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa gttcttcctc
    19081 gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt ctaaatcttt
    19141 tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc ctgccattac
    19201 ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat gcatatctgc
    19261 atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct gctgatcata
    19321 catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg cctttcggtt
    19381 ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc taaaaaagct
    19441 gataaataaa ggtttttctt actatatttc tatttccttg acagaataag gtttttatat
    19501 ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa caaattaatc
    19561 attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt tcgaaagatt
    19621 tattttttta atacagtctc aatataacta ttcaaacata aaataacgtt atttgattta
    19681 tttgtttata gtaaatttta tttagtaatc attgctttaa aatactacgc ttatataaca
    19741 gagaaaataa gcgttataat aaagaacagt attatttaaa atgacagaaa acattactat
    19801 taaagaaata aaaaacataa aaaatcttaa gaaatcggtt tcatttagct tttgagataa
    19861 aataatagta cagttttata cgctgtttca tatgtttaga aaatttgaat tagatttgaa
    19921 aactctaatt tttaaatgat aaaaaaagcg agaatgctgg ttttactgta tattaacctt
    19981 agataacttt cattttttta aaaaaattat gaaaaaaagg tagctattta aaagttgatt
    20041 atgtataata gaaacagatg ggagattatt atgatcttct gttacaaaaa agtgattctt
    20101 atcacgaaaa ggagtggtgc acgttgttaa cattatatac ttccccaagt tgtacttcct
    20161 gcagaaaggc tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa cgtaatattt
    20221 tttcagaacc attgaacagt tctgaattaa aagcgatttt acaaatgaca gaagacggaa
    20281 cagaagaaat catctcgaca cgttccaaag tattccaaaa actgaacatg gatttagatg
    20341 atctgccgtt acaagaatta ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc
    20401 caatcatgat tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt
    20461 tcctaccgcg cgatgtacga caattagaat tacgtcaagc acaattgatg gctggattat
    20521 agaaggataa tacgaaactt ggaaaaga
//
LOCUS       DAZKPO010000058        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000058 DAZKPO010000000
VERSION     DAZKPO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.058"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8NZ_001958"
     CDS             291..1430
                     /locus_tag="ACG8NZ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0539620.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8NZ_001959"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8NZ_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0539621.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8NZ_001960"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8NZ_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0539622.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8NZ_001961"
     CDS             3334..4302
                     /locus_tag="ACG8NZ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0539623.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8NZ_001962"
     CDS             4415..4693
                     /locus_tag="ACG8NZ_001962"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539624.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8NZ_001963"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8NZ_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0539625.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8NZ_001964"
     CDS             5150..5905
                     /locus_tag="ACG8NZ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0539626.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8NZ_001965"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8NZ_001965"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0539627.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8NZ_001966"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8NZ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0539628.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8NZ_001967"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8NZ_001967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0539629.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8NZ_001968"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8NZ_001968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0539630.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8NZ_001969"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8NZ_001969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0539631.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8NZ_001970"
     CDS             10642..11184
                     /locus_tag="ACG8NZ_001970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0539632.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8NZ_001971"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8NZ_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0539633.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8NZ_001972"
     CDS             12777..13679
                     /locus_tag="ACG8NZ_001972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0539634.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8NZ_001973"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8NZ_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0539635.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8NZ_001974"
     CDS             15122..15544
                     /locus_tag="ACG8NZ_001974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0539636.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8NZ_001975"
     CDS             16013..16243
                     /locus_tag="ACG8NZ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0539637.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8NZ_001976"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8NZ_001976"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0539638.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8NZ_001977"
     CDS             17654..17830
                     /locus_tag="ACG8NZ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0539639.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8NZ_001978"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8NZ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0539640.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8NZ_001979"
     CDS             18325..18708
                     /locus_tag="ACG8NZ_001979"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539641.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8NZ_001980"
     CDS             18877..19761
                     /locus_tag="ACG8NZ_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0539642.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8NZ_001981"
     CDS             19875..20111
                     /locus_tag="ACG8NZ_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0539643.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG8NZ_001982"
     tRNA            20199..20279
                     /locus_tag="ACG8NZ_001982"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG8NZ_001983"
     tRNA            20286..20357
                     /locus_tag="ACG8NZ_001983"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKPO010000059        20244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000059 DAZKPO010000000
VERSION     DAZKPO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.059"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1666)
                     /locus_tag="ACG8NZ_001984"
     CDS             complement(107..1666)
                     /locus_tag="ACG8NZ_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0539644.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1821..4055
                     /gene="pcrA"
                     /locus_tag="ACG8NZ_001985"
     CDS             1821..4055
                     /gene="pcrA"
                     /locus_tag="ACG8NZ_001985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0539645.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4072..6108
                     /gene="ligA"
                     /locus_tag="ACG8NZ_001986"
     CDS             4072..6108
                     /gene="ligA"
                     /locus_tag="ACG8NZ_001986"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0539646.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6214..6519
                     /gene="gatC"
                     /locus_tag="ACG8NZ_001987"
     CDS             6214..6519
                     /gene="gatC"
                     /locus_tag="ACG8NZ_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0539647.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6519..7991
                     /gene="gatA"
                     /locus_tag="ACG8NZ_001988"
     CDS             6519..7991
                     /gene="gatA"
                     /locus_tag="ACG8NZ_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0539648.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7988..9418
                     /gene="gatB"
                     /locus_tag="ACG8NZ_001989"
     CDS             7988..9418
                     /gene="gatB"
                     /locus_tag="ACG8NZ_001989"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0539649.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9428..10483
                     /locus_tag="ACG8NZ_001990"
     CDS             9428..10483
                     /locus_tag="ACG8NZ_001990"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0539650.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10594..11967
                     /gene="rlmD"
                     /locus_tag="ACG8NZ_001991"
     CDS             10594..11967
                     /gene="rlmD"
                     /locus_tag="ACG8NZ_001991"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0539651.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11970..12098
                     /locus_tag="ACG8NZ_001992"
     CDS             11970..12098
                     /locus_tag="ACG8NZ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0539652.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12538..12726
                     /locus_tag="ACG8NZ_001993"
     CDS             12538..12726
                     /locus_tag="ACG8NZ_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0539653.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12804..13817
                     /locus_tag="ACG8NZ_001994"
     CDS             12804..13817
                     /locus_tag="ACG8NZ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0539654.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13884..14918)
                     /locus_tag="ACG8NZ_001995"
     CDS             complement(13884..14918)
                     /locus_tag="ACG8NZ_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539655.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15033..17258
                     /locus_tag="ACG8NZ_001996"
     CDS             15033..17258
                     /locus_tag="ACG8NZ_001996"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0539656.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17276..19159
                     /locus_tag="ACG8NZ_001997"
     CDS             17276..19159
                     /locus_tag="ACG8NZ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0539657.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19140..20114
                     /locus_tag="ACG8NZ_001998"
     CDS             19140..20114
                     /locus_tag="ACG8NZ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0539658.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6817 a         3716 c         4346 g         5365 t
ORIGIN      
        1 aaaaaagaag aggctgggtt ttcccagcct cttctttttt tatagaatca tatagaatca
       61 agtagtgtag atgaatagtt gttgtaaaat agtttttcca tgttcgttaa aatgattgct
      121 tcaaatttgt gatagaagat ttagcctcga tttcctgctt gattcttggc aagtttgctt
      181 catcagtcaa gataaccaaa atatctccta cattcatgat cgtatcaccg tgagtcaaga
      241 tttcctgatt tcctctgcgt atagagatta gcagcatatt tttaggccaa ttgaagtctc
      301 gtaccattaa tccgtctaaa gaactctctg ccgttactgg aaaatcaaaa gttgtccttg
      361 caccttttag tgaggcggtc tgagtatttc caagtagacg ttccaataat gattcgtaaa
      421 ttggattacc gccgagaagg tcattgacga tataagcaac aagagcagcc aatcctaaag
      481 gcatcagatg attaagagag ccaaccattt ctgtcactaa aataattgct gtcagcggtg
      541 cttttccaat cgctgtgaaa taaccagcca tggcaataac gacaaaatcc ttgatgtaga
      601 ttggatccat cccgaaatca accataatgt ttccataaaa gctaccgatt aaagccccca
      661 atgtcaatat cggaaggaaa atcccccctg gaagaccccc accgtaagaa atcatggaaa
      721 aaacgaatcg aagaacaaac aaaccgataa tcataatgat tggcaaggaa gcatgtccaa
      781 aagctaaaac gatttgattg cctccgccta gagtactagg ccataaataa ccaattggca
      841 aaatcagcaa gaaaggaacg aacccgtgaa aataagctgg taaaggcaat cggttgtaaa
      901 attttggcaa agccagcaag acattctgat aaataaaacc taagatgcct agaagtactc
      961 ctaagatcac taaggaacca taatatttca acggcaaaga aggaaggtca cctagatata
     1021 aaacaggtct caagccgaaa aagtaaagag agacgaagtt agcagtgatt gctgaagcaa
     1081 atgatgtcag ccaaacaagt ggagaaaagg agtgatggat ttcttctaaa acaaagagta
     1141 atcctgcgat aggagcattg aatgctgctg caagtccgct acttgcaccg ctagaaatta
     1201 aaatcttttc ttctgaagat ggactccgaa acaattgtcc agctccttgt ccaatggatg
     1261 cgccaagctg tatagaaggt ccttctctac ctagaaaaag gcccgcacca actgaaagta
     1321 caccgccaat gaactttttc cataaaacag agaaccagtt cattgataat tccccgcgaa
     1381 gctgtcccac tacttgtgga atcccgcttc ccttgatatc cggttcattt ttgaccagat
     1441 aacccacgaa aaatgctgca aggatgcagc aaatagctag gccaattaag taaatcggct
     1501 gttcccgact catttcatag atacttcgaa ctaaatcgct tagcttttcg atagataatc
     1561 gaaacaggct aacgacgatt cctgtcagca gtccaacaaa tattcctttt agaataaaat
     1621 atatctgagt cttatctaat ttcttgattt ttcctttttt ttccattcaa acgcctcgct
     1681 ttcttttaat ttagcgcgaa atcaacgaat tgacaaggaa aaatccataa aaagaaacaa
     1741 atgattttga actagctagt ttaatgaaag ccaagaaaat gctataatta acaagattga
     1801 taaaagaaag gatgttgtta atgtcatcca aagctgaatt attaaacggg atgaaccccc
     1861 gtcagaaaga agctgttctt cacactgacg gaccattact gctaatggca ggtgcaggaa
     1921 gtggaaagac gagagtgctg acacaccgca ttgcctattt gattgaggaa aaagaagtca
     1981 atccttggaa catcctagcg attactttta ccaataaggc tgcaaaagaa atgaaagaac
     2041 gtgtcaatgc gattctcgca tctggaggcg aagatgtctg ggtatcgact tttcactcga
     2101 tgtgtgtgcg tattttaaga agagatgttg attttatcgg gtataaccgg aactttacaa
     2161 tcatcgattc ttctgaacag ctgaccttga tgaaacggat actaaaagaa ctgaacattg
     2221 atccgaaaaa atatgatcct cgaagtatat taggaacaat ctctcaagca aaaaacagcc
     2281 tgcagacccc tcaggatttt gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa
     2341 aatgttatgc tgcttaccaa aaagagcttc aatataatca atgtatggac tttgacgacc
     2401 tgatcatgaa tacgattcga ttatttgaag aacatcctga ttcactcacg tattatcaaa
     2461 acaaattcca ttatattcat gtagatgagt accaagatac aaatcacgca cagtatacgc
     2521 tagtcaattt attggcaggt agatttagga acctgtgcgt cgtgggagat gcagatcaaa
     2581 gtatttacgg ctggcgcggt gcagatatgc agaacatctt ggattttgaa aaagattatc
     2641 cagatgctgc agtaattttg ctagaacaaa actatcgctc cacgaaaaac atccttagcg
     2701 cagcaaacca agtcattgaa aacaatagca atcgaaaacc aaaaaacttg tggacagaaa
     2761 acaaagaagg aaacaagatc acttactacc gtgcagataa tgaacgagac gaaacccgtt
     2821 ttatcgtaga ccggatgcaa gaagagattc gaagcaatca tcggaattac ggcgactttg
     2881 ccatcttgta ccggacgaac gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa
     2941 atattcctta taaaatggtt ggagggcaca agttctacga tcgtaaagaa atcaaagata
     3001 ttttagcgta tttgaatgtg ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg
     3061 ttaattcgcc caaacgaggc atcggaccag gatctatcga aaaactacgc agttttgctt
     3121 ctcttcatga atggccactg cttgaagcag cgcaaaatgt ggatctggca aatatcggtg
     3181 gaaaagcagg acagcaatta ggtgcatttg gagagatgat ccaagaagtc actcagatga
     3241 tcccatactt gacagtgaca gaattgacga aagaggtttt ggatcgtagc ggatatctag
     3301 aagacttaaa aatccaaaat acattagaag cacaagcacg catcgaaaac ttggaggaat
     3361 ttttaacggt cacacaagaa ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg
     3421 atgcaccaga agaaaaacta acggtcttct taaatgatct ggcacttgtt tccgacattg
     3481 acaatttaga agaagatgct tcccaagtga cattgatgac tttgcatgca gccaaaggat
     3541 tagaatttcc agttgtattc ctgattggtc tagaagaggg ggtttttcct ttatctcgag
     3601 cattgatgga agaaagcgaa ctggaggaag aacgccgtct cgcatatgtg gggatcacgc
     3661 gagcagaaga agcgttgtat ttaacaaatg ctttctccag aacattatat ggacggacgc
     3721 aatataaccg accttctcgt tttgtagaag aaatcgatca ggagttactg gaaatcgaag
     3781 gcatgcgtcc tacgcctaaa aaaacgcctg tattcgctaa aaaaacagca tatagctata
     3841 aacagccaga gacagcggtt gttccctcta aaagtgctac tggaggggaa aataacagct
     3901 ggaaacctgg agataaagtc aaacacaaaa aatggggtct aggaacagtc gtccgagtaa
     3961 gcgggacgtc gaaggatcta gaactagatg tggctttccc aagtcaagga gtaaaacgtc
     4021 ttttggctgc ctttgcgcca attgaaaaag cttagtagtg gagggtaaat gatggaacat
     4081 gaaatgacga ttgacgaagc tgctaaacga gcagaagaac ttcgtacaag actgaaccaa
     4141 tggtctcgcg aatactacgt agaagataaa ccaacagtgg aagactatgt ttatgataaa
     4201 gaatacgcag aattagtagc aatcgaggaa caatatcctg atttgatcac gtctgattct
     4261 cccactcaac gagtaggcgg aaaagtatta gaaggttttg aaaaagtcac tcatgatatt
     4321 ccgctttaca gtttgaacga cgttttcagc aaagaagagc tgatagcttt tgatcaacgt
     4381 gtacaaaaag cagtgggacg tgtagttgac tattgctgtg aactaaaaat agatggactt
     4441 tccgtttctt tgcgttatga agatggaaat ttcgtgagag gtgcgactcg cggggatgga
     4501 actgtaggag aaaacatcac tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta
     4561 aaagaaccaa tgaacatcga agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc
     4621 caattgaacc aagacagaga agcagaggga aaagatatct ttgcgaatcc tcggaacgcc
     4681 gcagctggtt ctttgcgtca gttagattct aaaatcacag cgaaaagaaa tctggacact
     4741 tttttataca cagtagctga tttcggtcct atgcaagcaa aaacccagta tgatgcgcta
     4801 gaagagctag aaaaaattgg attccacacg aatcgggaaa aacgtctatg tcattcgatt
     4861 gatgaagtgt ggtcatacat tgaagaatat catgacaaac gtgtggattt gccctatgaa
     4921 atcgatggaa tcgtcatcaa agtgaatgaa ttctcgctac aagatcaact tggttttact
     4981 atcaaagcac cgagatgggc agcagcttac aagtttccgc cagaagaagt tgaaacgcta
     5041 atcgaaaaca tcgaatggac agtcggtcga acaggggtag taacgccaac agctatcatg
     5101 acaccagtcc gagtagcagg aacgacagta agccgtgcca gcctgcataa cggtgattat
     5161 attaagttga aggatatccg cttgaaagat acagttctga tttataaagc gggagacatc
     5221 atcccagaag tttcgcaagt cgtgttagat aaacgaccta aagacagcga agaataccaa
     5281 ttgcctacac attgtccagt ttgtggaagt gaacttgtcc atttggatga agaagtagcc
     5341 ttacggtgca tcaatcctaa atgccctgct caaatgaaag aaggtttgaa ccactttgtt
     5401 tcaagaaatg cgatgaatat cgatgggttg gggccgcgcg tactagaaca gatgtatgat
     5461 aaaaaactag tagcagatgt tgctgatctt tacaaattga cagaagaaga gttattgacg
     5521 ttggacaaaa taaaagaaaa atcagcaaat aatattttga cagcaatcga caacagcaaa
     5581 gataattcgg tcgagcggtt gattttcggg ttaggaatcc gacatgtcgg agcgaaagca
     5641 gcaaaaattt tagcagaaca tttcggtgat ctagaaacat tgagtaaaag tgactatgag
     5701 tcgattattg cgcttgatac gatcggtgat atcattgccg acagtgtagt cacttatttc
     5761 agtaacgaag aagtccatga attgatgaat gaattaaaac aagcaggcgt caatttcgaa
     5821 tacaaagggc ttcgaaatgc ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca
     5881 gtagtcctta ctggtaaact aacacgattt actcgtgaag aagcaaaaga gacgatcgaa
     5941 aatcttggcg ggaaagtgac tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga
     6001 gaagatgctg gcagcaagct gaccaaagca caagaattag gaatcgaagt ttggacagaa
     6061 gatcaaatgg cagatgcatt ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt
     6121 ttcagacaaa tttcaataaa caatttcaga cacggcactc ttgtgttaaa atagagagca
     6181 aagtttttaa ctcgtttaga aagaaggggt agtatggcga ttagtgaaga acaagtgaaa
     6241 cacgttgcca aattagcaaa acttgcgttc gcagatgaag aacttgcaag ttttaccgac
     6301 caattaggta aaatcatcga tatggttgaa atgctggaag aagtagatac ggaaggtgtt
     6361 ccatttacgt cgaatgtggt tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct
     6421 ggatggaacc gcgatgaatt attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa
     6481 gtacccgcaa tcatcgataa tggggaggct ggcgcataat gacagaatta cacaatcgtt
     6541 ctctagaaga actgcatcat atgcttgtat cgaaagaaat cactgtacaa gatttgacaa
     6601 aaacaacatt tgaacgaatc aaagaaacag agcctgaggt cgaggcattc attacattga
     6661 acgaagaaaa agcattggaa caggcaaaag ctttagatga aaaagggatt gatgaaagca
     6721 acgtccttgc tggtattcca attggtatca aggataatat cgtaacaaaa gatatcttga
     6781 caaccgctgc atctaagatc ctttacaatt ttgaaccaat ttatgatgca acggtcatgg
     6841 ataaaatcta tcaagcagat atgatccctg ttggtaagct aaacatggat gaatttgcta
     6901 tgggtggaag tacagaaact tcttatttca agaaaacaaa aaatgcttgg gacccaacaa
     6961 aagtacctgg tggctcttct ggtggttctg cagcagcagt tgcagctgga caaattccag
     7021 tttctttagg tagtgatact gggggaagta tccgccaacc agcagctttt aacggcatcg
     7081 tagggatgaa acctacttat ggccgtgtct cacgttttgg tttgattgcc tttgcttcat
     7141 ctcttgacca aatcggacca atgacccgta cggtgaaaga taatgcatta gcattgacag
     7201 ctatcagcgg ttatgacgaa aaagacggta cttcttcagg cgtttcagtt cctgattttg
     7261 cagagggact gacaggcgac atcaaaggaa tgaagattgc tttacctaaa gaatacttag
     7321 gcgaaggtgt ggcccctggc gtaaaagaag ctgtattgaa agcagcagaa acattccgtt
     7381 cattaggtgc tgttgtggaa gaagtcagct tgcctcattc aaaatacggt gtggctgttt
     7441 attatatcat tgcctcatca gaagcaagtt ccaatttgca acgattcgat ggtatccgct
     7501 acggttatcg ctctgaaaat gtgaaatcat tagatgatgt atatgtcaac tcacgttcag
     7561 aaggattcgg tgaagaagta aaacgccgta tcatgttagg tacattctcc ttaagcgcgg
     7621 gttattatga cgcttacttc aaaaaagcag gacaagtccg tacattgatc aaacgagatt
     7681 ttgaaaaagt ctttgcagat tatgacttga tcatcgggcc atcttcacca acagttgcat
     7741 tcggaatcgg tgagaacatt aacgatccaa tcacaatgta catgagtgac atcttaacga
     7801 tccctgtcaa cttggctgga cttccaggta tgtctgtacc ggcaggttta tcagaaggat
     7861 taccagttgg attgcagatc atcggtaatt attttgatga aaaaacgatg tatcaagcgg
     7921 catatgcgtt tgaacaagca acagaattcc ataaacaaca accagctatt ttaggaggga
     7981 aagacgaatg aattttgaaa cagtcattgg actagaagtc cacgtcgagt taaaaaccaa
     8041 ctcaaaaatt ttctctccag cgccagctca ttttggtgca gaaccaaaca gcaatacaaa
     8101 cgtgatcgac tggggttatc caggcgtgct gccagtaatg aataaagctg cgctggaatt
     8161 cgggatgaaa gcagctttgg cattaaactg tgaaatctct aaagatactc attttgaccg
     8221 gaaaaattac ttttacccag ataatccaaa agcatatcaa atttcccaat ttgatcaacc
     8281 aatcggtcat gacggatgga tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat
     8341 cgaacgtgtc catttagaag aagatgctgg aaaaaacatg catggtattg gcggttattc
     8401 ttacgttgat ttgaaccgac aaggcacgcc tttgatcgaa atcgtttctg aagctgatat
     8461 gcgttcacca gaagaagcat acgcttatct agaagcattg cgatcaatta tccaatttac
     8521 agaagtcagc gatgtgaaaa tggaagaagg ctctatgcgc tgtgatgcca atatttctct
     8581 tcgcccttat ggacaagaag agttcggtac gaaagcagaa ttgaaaaact tgaactcttt
     8641 atctttcgtg aaaaaaggtt tagcttttga agaaaaacgt caagcaaaag ttcttctttc
     8701 aggaggagaa atccaacaag aaacacgccg ttttgacgaa acaacgaata aaacagtttt
     8761 gatgcgtgtc aaagaagggt ctagtgatta tcgttacttc ccagagcctg atatcccgaa
     8821 attcgtgatc gatgacgaat ggattcaaaa agtaaaagct agtttgcctg agatgccagc
     8881 ttctcgtcgc gaacgctata tccgtgagtt tggacttcca gagtatgacg caatggtatt
     8941 gacacttaca aaagaaatgt ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc
     9001 caagcaagca tctaactggt tgatgggtga agtttctgct tacttgaaca gtgagaaact
     9061 ggaattagct gagacaaaat tgacacctga gaacttagca ggaatgattc gtttgatcgc
     9121 cgatggaacg atcagctcaa aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg
     9181 cggagaagcc caagaagtag ttgaaaaaaa tggttgggtg caattatctg atccgaacaa
     9241 actactgcca atcattaatg aaattttgga taacaaccaa caatctgtcg atgatttcaa
     9301 aaatggaaaa gatcgtgcgg ttggcttcct agtcggtcaa atcatgaaag caactaaagg
     9361 acaagcaaat cctggtgtcg tgaataagct acttaaagat gaattagcga aacgatagag
     9421 gctgaaaatg aaaaaagcac gcgtaattta taaccccacc tcgggaaaag aattattgaa
     9481 aaaaaatttg gcggatatcc tacaggcatt agaagaagca ggatttgaag ccagcgccta
     9541 tgcaactaca ccagaaccag attcagccaa gaatgaagca tgtagagtcg cagaactagg
     9601 ttttgatcta gtagtggcag ctggtggcga tggcacaatc aacgaagtgg ttaatggaat
     9661 cgctccgctt gaacaccgtc ctaaaatggc catcatccca gctggtacaa cgaacgacta
     9721 tgctcgagca ttaaaaattc ctagagataa tattaaagca gcagcagaag tgataaaaaa
     9781 acaccaaacc gtaaaaatgg acatcggcct ttctgataac agttacttca tcaatatcgc
     9841 tgcaggtggc tatttgacag aattgactta tgaagttcca tcagaactaa agagtatttt
     9901 tggttatctt gcttatcttg cgaaaggagc agagatgctc cctcgagtaa agccaatcaa
     9961 aatgcgcttg aagtatgatg aaggggaata tatcggaaat gcttcgatgt ttttcctagg
    10021 gctgacgaac tctgttggag gattcgaaca gatcgcgcca gatgccaaat tggatgatgg
    10081 gaagttttct ttaatcattg tgaagacagc aaatatcttt gagatcttgc atattgcagc
    10141 actcatgctg aatggcggtc gacatgtcga tgatccaaga ataatctata ccaaaacaag
    10201 cagtttgttc gttgaaacag aagatgatat caatcgacca gtaatgatca acttagatgg
    10261 tgaatacggc ggggaagcac cgatgcattt ccaaaatctt catcaacaca ttgagttctt
    10321 tgccaatacc gatcagattc ctgatgaagc aatcacaggt acagatgaag aagaattaga
    10381 ggttgtcagc aaagaattcg taaaagaagt cgaacgttta acggatgaag atatcgacgg
    10441 tgacggaaaa atagcagacg aaacacctaa tcaaaagaat taatcaaaag ccggtgctga
    10501 acgtagtaag gtcagtgccg gttttagttg tgtaattagc tgatttatag tagagttcag
    10561 taggattgaa aaataggctt aaaggagcaa aatatgacag aacagcaaat agtaaagaaa
    10621 aacgaacgat atacagtcga aatcgtcgac ttgagttatg aaggaatggg ggtagcaaaa
    10681 atcgatggtt atccattatt tatcgaaaat gcactccctg gagaagaagt agaaatacta
    10741 gtagtaaaag caggcaataa gtttggatat ggaaaagtcg aaacatttat tacacaatca
    10801 cctgatcgtc agcctgtaga aaacaatgca ttacttcgca taggtatcgc acctttagcc
    10861 catttgaagt atgaacaaca gcttgtcttc aaacaaaaac aagtagagca tgtaatggcg
    10921 aagattgcta agatgccaga agttcctgta cttccaacgg tcggaatggc agaaccattc
    10981 ggctaccgta ataaagcaca gatccctgtt cgccgtatcg atggacagtt agccaccggt
    11041 ttttacaaaa agaatagcca tgatctagtc cctatcgaag atttttatat ccaagatcct
    11101 gtcatcgatc aagcaatcaa agtagtcaga gatatcttgc aacggttcca agtacgagga
    11161 tataatgaag aaaaaaatga aggacagctt cgtcacatca ttatccgtcg tggtcattat
    11221 tcccatgaaa tgatggtcgt tttagttact cgtaaagaaa aattctttaa atccaaggaa
    11281 attgctgaag ctattcatga agaattgcca gaagtggttt cggtcatcca aaatatcaat
    11341 gaagaaaaaa caaatgtgat tttaggagac aaagaaaaag ttttatatgg cagagactat
    11401 atcgaagatc agctattagg aaaaacctat cgaatctcag caaagtcatt ttatcaagta
    11461 aatactcaac aagcagagta tctgtaccaa acagccatcg agtttgccga gttagataaa
    11521 aaagacattg tgatcgacgc ttattcagga ataggtacga ttggcttgtc tttagcagac
    11581 agagtggata aggtgtatgg gatggaagtt attccagaag caattgaaga tgcacagttc
    11641 aatgctttaa ataataaaat cgaaaatgtt cattatgaag tcggaaaagc agaaaaagtt
    11701 atgaaaaaat ggcaagaaaa agggatcaaa ccatctgtga tcgtagttga ccctccacga
    11761 aaaggattag atcatcattt tattgaatca gcaatcgaaa tgtcaccaga acgaatcgtc
    11821 tatatctcat gtaatccggc aacttttgca agagatgcta agttatttgc agaagcaggt
    11881 tatgccgtga agaaagttca gcctgtagat cttttcccgc aaacccatca tatcgaatta
    11941 gtcgcttcat tcgttaaagg agaataaaaa tggaccaaaa agaaaaaatg aaagaattga
    12001 tcaaaaagaa acaaggtgga ggccgatcca aacaaatgga aacacctaag aatgatcgta
    12061 aaaacatgag aaaaggccct aaaatcttta ataaatagac gtgctaaaga aagactaaga
    12121 agtgttaact tttgttattg tttcttagtc tttttagtta gtctacatta ttttggcgat
    12181 ttgccaataa ggactgttga gttgtgcaaa atcaaatctg ggtcagaatg aacgtcattc
    12241 tgtctcagat ttttcgttat ttcaatttga ttaactgatt gatcaaaaag atttttattt
    12301 tcatattctc aaatgattca acactaaata tacatgtttg acgcggaaat tattattcga
    12361 ttttggcggc atcagggggg atgacgacgt agcagaccca ttattttact caagaaaatg
    12421 tccgtgccta ttatttagaa tacgatagtg aacgtgctgg gagttttgtt cttttagaga
    12481 aagtctctga agataaatta gttgtcttag attttgactg taacgaaaaa tggcgaaatg
    12541 gaagagcacc agcaagtgat tgaccggatc tacgaagcga cacgatatct gcctttggat
    12601 cgtttgtgct taagtcctca atgtggattt gcttcaacag aagaagaaaa tgttttgttt
    12661 gaagaacaat ggaaaaaatt tcttttgtca aaagcattgc agaagaagtt tggggaaaat
    12721 cattaaatca tctaggagag cttgatagga aataacacta tggtataatc agaatgctta
    12781 atactaacta ctgagggagc cttatgaaaa agaaaattct taaatttatc ggatggttct
    12841 tcggaatcat tcttgggatc gcgtttgcaa tctttcttgc cttccagctt tcccccagac
    12901 ctggagcatt cttgatcaac catatgtttt caaatactgt tcaaatcact gataaaaaaa
    12961 cgttcgatca agcagaaaaa aacgtggcga aaaaaacaga tttgacgtat gagtccaagt
    13021 ataaagagaa tacttatgac atctactatc caaaaaacag caaggggcct gttcctgttc
    13081 tattttgggt acatggtgga ggattcgtcg gcggagataa atcgggtgtc aaagaatttg
    13141 cgacaaaatt agtttcagac gccaacatcg cagtggttgc gatgaattat gaattagcgc
    13201 ctgattcaga ataccctaat caagttcttc aagtgaatga actgatcaaa gatttattag
    13261 ctgaagaaaa agacgacaaa ttgctagaca tggaacaatt attttttggt ggagatagtg
    13321 caggttcaca aatcgcattg caatatgcag ctatacaaac gaatgatgcg tatgctaaac
    13381 aaatgaaaat caaacagcta cttccaaaag atagtctgaa aggaaccatt tcttattgcg
    13441 gacctgttga tttgaagcta acagcacagc agcattcgga tgatcgattt atgaagtttt
    13501 tcgtcaaaac cgttgcgtgg tcgttgattg gaacaaaaga ttggaaaacg agtgagcaat
    13561 tgaaagaagc tagtgtggct gaccatgttt ctaaggaatt tccgccaacg tatataactg
    13621 acggaaatgc atactcattc caggagcaag gtcaagcatt agaaaatcgg ttgacagaat
    13681 taaaggtacc cgtgcaatcc ttgttttata cagatacaaa aaaagagatt acccatgaat
    13741 accaatttga ttacacgcta aaagaatcgc aaaattgtta ccagcagaca ttggattttg
    13801 tgaataagta taagtaagag gttgtgaaaa agccgtttcg ttttgagtat gaagaaacga
    13861 agcggctttt cacaacctct ctttcaatac ctttttgttc ttgggataaa actttcccta
    13921 acaatcaatc gtgcaggata agtcaaaatg attggttcgt cccgtatacc gtcaattcgc
    13981 tccaatgcca ttcgaacagc tgcttgccca attgatttcg cattgatttg aacactggtc
    14041 aagccagggg agaggtttgc gattgtctcg atattatcaa agcttgctac aacaatctct
    14101 tctccaattt ggatattttt ttgtttcaat gcccgataga caccaattgc cattggatcg
    14161 ctcccgacaa gaatggcagt aggaagaggg gattcatttt ctaatagatt agtcgcaagt
    14221 acctcacctt gtttttctgt ccagccacct agatgataag acatatattt ttctaagtga
    14281 tgatcgatca taaaatgctg ataggcacgc atcctctttt catttataga aatctgcttc
    14341 ccaccgaatt catccatatc gactttatat cccccgatat aggctattct tttatgacca
    14401 ttttctaaga acaattccag tacggtttta gtcatccgtt caagatctgc atacaccata
    14461 tcgacctctt gctggatgtc agggttatcc acgacgacta tttgcatgtt ttgttttaac
    14521 aaggcggtaa tcgattcttt cgtaacagtc ccaacaacga tcaccgcacc taactgatct
    14581 agatcttccc attgtttagg attatcagat agattataga ttcgattcat ccctaaatgc
    14641 agacgtcgag cttcttcttc catccctact cgcaaatgtt tgtaatagat atcctgttct
    14701 tcgctcgctt tgccgatcgt agagatcaca ccaatcgaag gcattctctt tttgatatat
    14761 ttacgagtac tgatgtcata atccaacgca gcagctgcct ctactatccg ttgccgagta
    14821 gtctgagcca catggatcgt ctgatcgtta ttcaacacac gggaaactgt agcaggagag
    14881 acatttgcct ttcgtgcgac atctctaatt ccaaccattt ttacacactc ctcaaaaaaa
    14941 taaaaaattt ttattgacaa aattgtttag taaacagtat acaggaatta taaaaaaaag
    15001 taaacggttt ccgttttgtt gggagtgaaa tgatggatat tacgcataga aaaacagata
    15061 aaaatttcat cttatttgat gaaaagagcc gagtcttcca tttacgaaat acatttcttt
    15121 cttatctgat aaagatagaa gaaagcaatg tattggcgca tatttatttt ggcaaaccag
    15181 tcaagcagta caaagacaat aaaaattatc cccgaagaga tcgtggattt tcaggaaatg
    15241 ttcccttaaa tcctgaccgg tctctgtcga aagatacact accgcaagaa tactcttctc
    15301 atggcagcat ggactttcga acgcccgcat cgatcattca gcgcaagaat ggatcagact
    15361 tactggatct gagatatgac agccattata ttacagatgg gaagccagag atcgaaggct
    15421 tgcctcaaac ttatgtgatg gacaagtcag aggcacaaac gttggtgatt tcacttaagg
    15481 atagagaaac agcgatttat ttcgatttgt tctataccat atttactgat agagcagtga
    15541 tcacacgatc tgtaaagata agaaatgaaa ccggagaaac aatcaaacta gaaaaagcgg
    15601 ctagtttcca gctagacttt gcccatacta ggcgatttga tgaagtcatt gctttgccag
    15661 gagcacatgt caatgaaaga cagatttcac ggcaatctgt tttatctgga acaaaagtat
    15721 ttgaaagccg tcgaggaacg agcagtcacc atatgaataa ctttattgct ttagttcatc
    15781 atcatacgac agaaaatact ggagaagcga tcggacttca atttgtttat tccggcaacc
    15841 attctttcga acttgaaaaa gatcagatca atcaattgag agtcgtcggt gggatcaaca
    15901 gccatcgttt ttcgtgggaa ttgaatgcag gccagtcttt tcaaacacct gaaatgatcc
    15961 tttcttactc aagtcaggga ttgaacaaaa tgagtcagat ccaccatgag ttgctacgtg
    16021 aaagaatcgc aagaggtcgt catcaatttg ctgaacggcc gattctggtc aataactggg
    16081 aagccactta ctttgacttc aatagtgaaa aaatcaaggc gatcattgat gaagcaaagg
    16141 agctaggcat cgaaatgttc gtattagatg atggctggtt tggaaaaaga gatgcggaca
    16201 attcttcatt aggagactgg ttcgagtatg aaggcaagtt gacaaatgga ttacgtgaaa
    16261 tagcagatta tgctcatagc aaaggattga aatttggttt atggtttgaa cctgaaatga
    16321 tctcggttga ttctgaactg tatcgaacac atcctgattt tcttatgcag gagccaggta
    16381 gaatgccttc tgcttccaga tcacaacatg tactggattt tacaaggtta gatgttcggc
    16441 agacgattga gaaacaaatg agaaagattt tagatacgat tcctcttgac tacatcaaat
    16501 gggatatgaa ccgttcgctt tcagatgtct attcgattac ccttgaccca cagagacaag
    16561 gagaagtagc gcatcgttat atgctcgggc tttatgaact acttgaacat ttgacaactg
    16621 attatccgga aatcttatgg gaaggctgtt ctggtggagg tggacggttt gatgccgggt
    16681 ttatttatta tatgccgcag tcatggacaa gtgataacac ggatgcagtg gagcgtatga
    16741 aaatccaata tggaacaagc cttgcttatc cgatttcgtc catcacagct catgtatcag
    16801 ctgtaccaaa tcatcagacc gacagatcta cttcgttgaa aacaagagga gaaacagcta
    16861 tgagcgctgt ttttggttat gaactggatt tgacaaagct gtctcctgaa gaagaaaagc
    16921 aagtaaaaga ccaaattata tcctatcaaa cgattcgtcc ggtgatccag tatggccatt
    16981 attatcgttt ggcgagtcct tttgaagaaa atattgccgc atggatgttt gtgtctccta
    17041 aacaagatga agctattgtg tttttaggga ggatactagc ttcagctcaa ccagcgttcc
    17101 atgaagtata tctgatgggg ttagatgatg aggcacttta tcaggaacag acctcgaagc
    17161 ggatattttc gggggccgaa ttgatgacag ttggacttta cttccccgat tttcaaggtg
    17221 atttccaaac agaactgctt catttcaaaa agttatgaga gagaaggaaa aaagtatgaa
    17281 agcaaatata aaatggttag atgatccaca agtttttaga gtgaaccaat caaatgcaca
    17341 tagcgatcat gtttgttatg caacaaggga agaagtagaa accacatcct cccttaaaca
    17401 gtctttaaat ggatcatggc gtttttgtta ttcgccaaat ccttcggtac gaccaaaaac
    17461 attttataaa aaagattttg atgcctgttc ttttgatgaa atcactgttc ctagtcatat
    17521 tgaattagca aattatgaca agatccatta catcaatacg atgtatccgt gggagggaca
    17581 tgtatttcgc cgtccccctt tcacgacaag ccctgagaaa gaggcggaag ggatcttttc
    17641 agctgctaaa gacaatccgg ttggttcgta tttgacgact tttcgattag aagaaggtct
    17701 aaagggaaaa caaatctcga ttcattttgc tggtgtagaa caagcaatgt ttatttggtt
    17761 gaatggtcat tttatcgggt atgccgaaga ttcgttcact ccgtctgaat ttgatttaac
    17821 accttatttg gaagaagaaa atattctagc tgtggaggtt tacaaaagaa gtacagctgc
    17881 attcttggaa gatcaggact ttttccgatt ttttgggatc ttcagagatg tcttcttagt
    17941 tgctcgagga caaaaccatt tagaagatct ttggatcaaa cctcgcttag atgacaaatg
    18001 tcaaaatggc aaacttgaag tagatttgtc ttttgagcaa gtagccaaaa acagtcagct
    18061 gaatgtagcg atactagatc ctgatgggaa ggaaatctat caacagcaat atccgttaaa
    18121 agagcaaatg acgattcgca tcgatggatt tgaaaatgtg gcactgtggg ggcattttca
    18181 gcccaattta tataagcttg aattggagac gatagacgaa gaaaaaagag taacggagta
    18241 tattgactat tccttcggtt ttcgtaaaat cgaaatcaaa gataaaatca tctacttcaa
    18301 tggaaaacgg atgattatca acggggtaaa tcgacatgag tggcatccga aaaaaggacg
    18361 cgccatcact atagaagata tggaaaaaga tttggctatc ataaaaaatg caaagatcaa
    18421 cggcgtacgg acatctcatt accccaacca gatcacttgg tattatttat gcgaccaagc
    18481 aggaatccat gtgatggcgg aaacgaacct cgaatcccat ggctcatggc aaaaaatggg
    18541 cgcatttgag ccttcttaca acgtaccagg tgcagtacct cagtggcagg cggctgttct
    18601 tgatcgagca aaaacgaatt tcgaaacatt aaaaaaccat ccaagtattc ttttttggtc
    18661 tcttggaaat gaatcttatg cggaagaaaa cattcgacaa atgaacgatt acttcaaaaa
    18721 agtagacccc gatcgacttg tccattatga agggaatttt ccaaatccag cattcgaaga
    18781 ttcgatttca gatgtaaaaa gctgcatgta tgcgccacca gaagagatca ttgactacct
    18841 tgaaaataaa gcggaaaaac cgttcatctt atgtgaatat atgcatgaca tggggaattc
    18901 gttaggtgga atggattctt acatgacgtt gcttgatcgt tatgaaatgt accaaggagg
    18961 ctatatatgg gattttaaag atcaggcgat ccaagtgact gatccaatca caggcagaga
    19021 agtgatgcgc tatggcggtg actttgacga taaaccaagt gactatgaat tctcagggaa
    19081 tgggatcgtt tttgcagatg gacaagaaaa acccgccatg caggaggtaa gatattatta
    19141 tgaaaaatac agtaaataaa agtcatatgg atacggaaaa agttgcaatc gtcttcggcg
    19201 actgtacatt aggtgtcaaa tcggggaata cgcattatat tttttcttat acaagaggcg
    19261 gactggaatc gctcaataaa aacggaaaag aatggctata ccgggaaaca cttccagttt
    19321 tttggcgagc aacgaccgat aatgacagag gtagcggatt tggatttaaa tccggtatgt
    19381 ggttaggagc cggattgtat ccaaaagtca aagaaataga agtactggtg gacaataaaa
    19441 agatcgattt gccgattgct ccagtaaata atcaatattc aagtaatgaa tacgctgaaa
    19501 cggttgaaat catttttaca ctggaaatca acacagtacc aaaaacccaa gtgacaattt
    19561 cctaccaaat cgctggagag ggtaaaatgc ttgtcaatat gcattattat ggacaaaaag
    19621 gattaccgga attacccgca ttgggtttaa ggttcattat gccaacgaaa gcaaaatttt
    19681 ttgagtatga aggcctatca ggggaaacat atccggatcg gatggctggt ggtgtcgaag
    19741 gtatttataa agtggcagga ctccccgttt ccccatacct tgttccccaa gaatgcggca
    19801 tgcatatgga aacgaaatgg gtagaactaa caagaaatca aacgttggat aatacggata
    19861 aagatcaaga agatttttcg ttgcgatttg aacagcagga tacgccattt gctttttccg
    19921 ctttaccatt caaagcagaa gaactggaac aggcaacaca tatagaagaa ctaccgccag
    19981 aaagacgaac ggttcttacg atttttgcaa aagtaagagg agtcgggggt atcaatagct
    20041 ggggagcgga tgttgagtca ccttaccgaa tcagcagcga agaggaccat gaaatcggct
    20101 ttattattca gtagcaatct ttagattatt atttgaaaaa cggagtttac aagacaaagc
    20161 tgctttattc tatatgtcat gaacaagaga gtaaaaaagt tctcagatac ttttgttcat
    20221 gactattttc ttcaataaaa ccaa
//
LOCUS       DAZKPO010000060        19694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000060 DAZKPO010000000
VERSION     DAZKPO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.060"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8NZ_001999"
     CDS             complement(<1..210)
                     /locus_tag="ACG8NZ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0539659.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG8NZ_002000"
     CDS             complement(216..446)
                     /locus_tag="ACG8NZ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539660.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG8NZ_002001"
     CDS             1063..1725
                     /locus_tag="ACG8NZ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539661.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8NZ_002002"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8NZ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0539662.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG8NZ_002003"
     CDS             5145..5459
                     /locus_tag="ACG8NZ_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0539663.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG8NZ_002004"
     CDS             5472..5846
                     /locus_tag="ACG8NZ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0539664.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG8NZ_002005"
     CDS             5856..6200
                     /locus_tag="ACG8NZ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0539665.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG8NZ_002006"
     CDS             6271..7623
                     /locus_tag="ACG8NZ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0539666.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG8NZ_002007"
     CDS             7683..8825
                     /locus_tag="ACG8NZ_002007"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0539667.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG8NZ_002008"
     CDS             8850..9104
                     /locus_tag="ACG8NZ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539668.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8NZ_002009"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8NZ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0539669.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG8NZ_002010"
     CDS             10541..10678
                     /locus_tag="ACG8NZ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0539670.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG8NZ_002011"
     CDS             10683..10904
                     /locus_tag="ACG8NZ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539671.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG8NZ_002012"
     CDS             10917..11417
                     /locus_tag="ACG8NZ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0539672.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG8NZ_002013"
     CDS             11514..13361
                     /locus_tag="ACG8NZ_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0539673.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG8NZ_002014"
     CDS             13364..14260
                     /locus_tag="ACG8NZ_002014"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0539674.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG8NZ_002015"
     CDS             14310..14699
                     /locus_tag="ACG8NZ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0539675.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG8NZ_002016"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG8NZ_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_002017"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8NZ_002017"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0539676.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACG8NZ_002018"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACG8NZ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAZKPO010000061        19602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000061 DAZKPO010000000
VERSION     DAZKPO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.061"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(496..1449)
                     /locus_tag="ACG8NZ_002019"
     CDS             complement(496..1449)
                     /locus_tag="ACG8NZ_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0539677.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1472..2227)
                     /locus_tag="ACG8NZ_002020"
     CDS             complement(1472..2227)
                     /locus_tag="ACG8NZ_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539678.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2224..3183)
                     /locus_tag="ACG8NZ_002021"
     CDS             complement(2224..3183)
                     /locus_tag="ACG8NZ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0539679.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3176..4126)
                     /locus_tag="ACG8NZ_002022"
     CDS             complement(3176..4126)
                     /locus_tag="ACG8NZ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0539680.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4276..4728
                     /locus_tag="ACG8NZ_002023"
     CDS             4276..4728
                     /locus_tag="ACG8NZ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0539681.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4817..7084)
                     /gene="gshAB"
                     /locus_tag="ACG8NZ_002024"
     CDS             complement(4817..7084)
                     /gene="gshAB"
                     /locus_tag="ACG8NZ_002024"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0539682.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7271..8269)
                     /gene="ftsY"
                     /locus_tag="ACG8NZ_002025"
     CDS             complement(7271..8269)
                     /gene="ftsY"
                     /locus_tag="ACG8NZ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0539683.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8280..9089)
                     /locus_tag="ACG8NZ_002026"
     CDS             complement(8280..9089)
                     /locus_tag="ACG8NZ_002026"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0539684.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9098..12679)
                     /gene="smc"
                     /locus_tag="ACG8NZ_002027"
     CDS             complement(9098..12679)
                     /gene="smc"
                     /locus_tag="ACG8NZ_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0539685.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12699..13385)
                     /gene="rnc"
                     /locus_tag="ACG8NZ_002028"
     CDS             complement(12699..13385)
                     /gene="rnc"
                     /locus_tag="ACG8NZ_002028"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0539686.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13733..15514)
                     /locus_tag="ACG8NZ_002029"
     CDS             complement(13733..15514)
                     /locus_tag="ACG8NZ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0539687.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15540..16454)
                     /locus_tag="ACG8NZ_002030"
     CDS             complement(15540..16454)
                     /locus_tag="ACG8NZ_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539688.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16481..17443)
                     /gene="opp4B"
                     /locus_tag="ACG8NZ_002031"
     CDS             complement(16481..17443)
                     /gene="opp4B"
                     /locus_tag="ACG8NZ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0539689.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17443..18387)
                     /locus_tag="ACG8NZ_002032"
     CDS             complement(17443..18387)
                     /locus_tag="ACG8NZ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539690.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18388..19392)
                     /locus_tag="ACG8NZ_002033"
     CDS             complement(18388..19392)
                     /locus_tag="ACG8NZ_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539691.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5416 a         4227 c         3472 g         6487 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttagaag agattgtgac aactgcgatt
       61 tgttttgagt aataagaacg ctggacagaa aatagcttct catattttct agtctagtgg
      121 acttattgcc gagaagcagc agatggaaca tcgtttaaag aagagatcgg tacaaaataa
      181 aaatatctaa atttaagttt tatctaagtt tcgctttgta cctttgattc aaatcaaagg
      241 aggcgatctt gatgctgaaa aaatggatca cactattttt tgccactccc cattctagaa
      301 tcaacccgta tgatgtagat aatgaattca cgttgaaaaa agatttctca aataaataaa
      361 aaccatccgt aaaatcatca atacgatttt acggatggtt tttgataact tctttgagga
      421 taggctcttg ctatttaaga atgtaagaaa cattattgtt acatagtatg agagtttaat
      481 cctctgttat ttatgctatt taaatgcttg attcacatct tcgatcatca gtttcattga
      541 ttctaaaccg cccccgctta aataccagac atctggttca agggagatga tctgctgatt
      601 tttaccagca ttcgtttgtg ctaccagttc attggcagaa atatcatctt tgctgtcgtc
      661 tccaccaata gcttttgttc tgtctactac aaataagata tccggatttt tttccagcac
      721 atattcataa gaaacacttt gaccgtgagt ggatgcttcg atttgatcat ctgcttgttc
      781 aaagccaaat aaatcatgga tgaaaccgaa tctagaacca gatccgtagg ctgataattg
      841 cccttcattc acaagtgtga ctagtgcttt tttgtcagta gcttctgctt tctttttcgt
      901 ctgatcgatt gcactagata attcctctaa ttgagtttta gcttcttctt gcttaccaaa
      961 gatctcccct aaagcagtga cattttgctg gaaagattcc catggatttt tcgcatctaa
     1021 agaaaggaaa atggtcggag caatttgact tagatctttt tgaaaatctc tttgtcggcc
     1081 agaaatgatg attagatctg gctgaagctg attaattttt tctaagtctg gttcttttat
     1141 tccacctgct gattctactt ttttatagga tgaaagatac tcaggaaggc tgcttgtagc
     1201 tgctccaact accttatctc caacaccaag agcatccatc gtatctaaag aaccattgtc
     1261 gaatacgacg actttttctg gattatttgg cacctcgatt tggtctccat tactatctgt
     1321 tatcgtcaat gtagtttcag tttgtgtgct gctttttttt gtgtctgcct gactggtgtt
     1381 gttgctattt ccacatgctg ctaaaccaaa tgatgctaga atcaagatac ctactactaa
     1441 ttttttcatc tcttctctcc taatgttagt tttagaaata aagacagaat ctgcggcctt
     1501 caatctcaca aatccgtatg tccatttcgt ataattcatc caatacatct ttttttatca
     1561 tttcttgggt cgttccttgc gtaaataact gtccatcttt cattgcaaca atttcgtctg
     1621 cataacttgc agcaaagttg atatcatgaa gaacaataac aattgttttc ccgatttctt
     1681 gtactaaatt ttttaaaatc tgcatgattt gaaccgcaaa attcatatct agattgttca
     1741 aaggttcatc cagtaaaata taatccgtgt cttgcgctag caccattgca atatacgcac
     1801 gttggagttg accgcctgaa agagtgtcaa tcgattgatc cgctatttct tctaacccaa
     1861 tgtgttccag tgattcctga acgatttcct cgtcttttgt cgtcaatttt cctttcgtat
     1921 aaggaaatcg tccaaattct actaattctt tcacggtgat ttggacattt atttcattcg
     1981 attgtcgtag gatcgataat tttttggcta attcattgga tttccaagcc tttacttcgt
     2041 tatggtctaa atagatttca cctgtatctt ttggtatcaa acgactgatc atcgacaaca
     2101 ccgttgactt tccagcacca tttggaccga taaaagcagt caacttcttt tcaggaatcg
     2161 ttaactgtac atctttaacc actgattttc catggtacag tttattgacc ttttgtaaat
     2221 caatcatagg atactcttcc tttcgcgaat caattttgtg acaaaataaa ttcctccgac
     2281 aaattcaacg acgacactca ctgttgtatt ccagccgaaa atctgctcta ctgcgagctg
     2341 tcctcctgtc agaaacagca tagctaccag aaagcttccg ataaatagct gaccatgacg
     2401 ataagtagaa aagacacgat aactgatatt tgcgacaata aaccctaaaa aagtaactgg
     2461 acccacaaga gccgttgatg tccctgtcaa aagactgaca caacataaaa tcaaaaattc
     2521 aaaacgatct gtgtctatcc cgaggtttac tgcttgttca tttccgagat ggaaaacgtc
     2581 tagcacatgt cgatatcgaa ataacaggta ccagcagccg ataatgatca aagaagcagg
     2641 caaaagatac tgactctgga tattgccaaa gcttgcaaat aatctgcctt gaagcaaatc
     2701 atattcattc ggatccatga tgacttgtaa aaaggtactg acactaccaa aaagcgtacc
     2761 taaaatcatc cctgacatca gtaataaaaa aagattcgct ctcatttttt tcaaaagaaa
     2821 gaaaaataac aatagactca gcccgaccat cagcccaacg ttcaataaga aaaaaactgt
     2881 acctgtctgc tgaagcatcg tcactcctcc gataaagaag aaaaggaccg tttggacaag
     2941 gacatataac gactcaagtc ccaatactga aggagtcaaa aactggttat gcgtaattgt
     3001 ctgaaagctg attgtggcac tgctgacagc tagcgagaca aggataaatg caaaaatttt
     3061 cttactcctc aatgcacatg caaaatgcca gtttccatat gtatggatcg tcaagtacat
     3121 tcctgtaaca aataaaacaa gaacaagcat accaataaaa atagatggct tttttttaag
     3181 catatcgtcg tccccttatc agaagataaa ggaagatagc cccaccgatg attccaacga
     3241 tcacactgac tggtatttca taaggaaaaa tcaacacgcg agcgacaaga tcgcaaaata
     3301 ctaagaacaa actacctgct aaagcagtta cgccaattgt tttgtgcact tgatctccat
     3361 atattctaga gacaagattc ggaatgatca ctccgataaa aggaatgctt cctaccatga
     3421 tcaacatgac tgcactagcc aatgatacta agccaatgcc taaaaactgc atcttctggt
     3481 aatctattcc cagatttact gcaaacgatt cgcccagacc aatgatcgta aatcggtaag
     3541 caaatagcca aaccaagagc aacaatggaa caacagcata caataactca taatttcctc
     3601 gtatcacagt cgcaaaattc ccttgaagcc atgaagtcat attttgtacc aactggaatt
     3661 gataagcaaa aaaagtagca actgaaccaa tgatattccc taacatgact ccaatcaacg
     3721 gaagcatcaa ttgatttttc tggaggaaca atcgcgtaag ggataaaaaa agcaatgttc
     3781 ctgcaaaagc aaagcaaaat gctgtcaagg atcggacaat caaggatgca ccaggtagga
     3841 aaatcatagc cactaaaata cctaaacgcg cactatccat cgtgcccgca gtcgttggtg
     3901 aaacaaattt attttgggtc aaatgttgca tgatcaaccc acaaatacta ctggtcgctc
     3961 ccacaaggat caaacaaatc gttcgtggca aacgagtcac tagcaagatt tgtaagcttt
     4021 gcccatctaa ctggagcagt tctgacaatt gtacattcga tactccgatg aacaatgaaa
     4081 tactacttaa aataactaat aatgctacta gtcgtccgta gcgcatccaa tccacctttc
     4141 taattgagaa tgattttcat tcttatttta agtgaatcgt tgaacaaaag caaccgcttt
     4201 ttcatatgtt ttaggtatta ttgggaatat gctataatcg taagaaagac aaagagaaga
     4261 aggagggtat tttttatggc acaagatgat gtaaaaagcc atttggataa gggactttat
     4321 ggtacacctt tagtgaaccc tgaagaacag cataaatatt tagggacatt cagggaacgc
     4381 tgctatctta gtatgactgt tgctgaaatg atccaaccaa aaaacaaaga aaacttttta
     4441 aaagaactca aaaaacatcc agatgcttcc atactcttga atggggctct acccctttct
     4501 gtccaaaacc aatatatcca gctagcaacg aaaaacaata gccgtttcac tgtagtcaac
     4561 gattacgtca ctgcaaatcc tgaggaattc ggattgctct taacagcaaa agaagcagta
     4621 aatgaacctg tgattgatat cgaaaaaaaa tacccagaag aaaactcttc gccagctgaa
     4681 aaagaaaaac caaaaaagaa aaaaagcttc tgggatcgtt tattttaaaa cgaaaaaaca
     4741 cataaatttt tacatgcaaa aaaagctttt gatcagattg acatcaaata ggacaatctg
     4801 tcaaaagctt tttcttttaa aatacttcag gatataacaa tttcaataca ttcatcgtca
     4861 atcttctgcc ttttcctgca aaaggataaa catgcatatg catggctgga ttgaaatttg
     4921 cttcgatgat cccatatgct tttttatcag ttgtaggatc gatttcttta tctggtataa
     4981 tcaaatcgat accactaatc ttagctccta gtgcttctac cgcagaaacg gcgatttttt
     5041 tgtaagcttc agaaaattca tcagtcatat cgatcgaatc accacctgta ctgatatttg
     5101 agttctccct tagataaaca atttgattgg cttggggaac tgattcgatc gtcaaccctt
     5161 gttctttcaa catcaattgt tctaattcac ctaattggat caattctaaa ggtgcacgat
     5221 gattcgttcc acgcaaaggg tcactatttt tttcttcaac aagttctttc actgttcgga
     5281 tactgtcacc aataacatta gcagggactc gcaacatgat ggcttggact tctccgtcga
     5341 tcacaaagaa acgatattcc gttcccggca taaattcttc tactaaaacc gaactgtctt
     5401 cacgaaaagc aatggctaaa cctgcttgat aatcttccag actagctcct tccttgaaaa
     5461 tcgtgatacc taacccgtaa ttggttgatt taggtttgat cacaaatgcc tgctcggcaa
     5521 atctcggata agcttttacc gcttcttcca tggatgaaaa ttctgcgcct cctggtaccc
     5581 ggaacccagc ttcttttaag acttttttcg ttactgtttt gttttccatg ataagtggga
     5641 cgatatagct gtccttactt gtcatgtttg catttttcac atattctaca tggtcttgga
     5701 attgcagacg caaaaattga tccgactcat ctaatacttt gacttggaca cctttttgga
     5761 tggcatcgaa cataaatatc tgagtagaaa gttccatttc gcgaaaaccg gccaactgat
     5821 agggacgttc atggctttct ttataatatt gatgtcccaa tgccacagca acttcttgct
     5881 gactcgatag ctgtgcttct tgatagagtt tcgctgctaa cgtttgttca ggatgatcca
     5941 tttgagtcaa tgcttcctct actaaataaa gagaatccaa ccaatccaat tcaaccagca
     6001 tttgcttcat tccttcaaga atttctctac cttcttgatg aaattctgtt tgatctgatg
     6061 gttgttctag tgctactttg ttatttcgag tcgtaccttc tgctacccat tggtcacagt
     6121 cttctttttc ttctgtccat aacatgtaca tcaggaataa ttgtaagaac cgaacttgct
     6181 ctggattgat acccaatctt gcataaggat tcaaatcgat attccgcaat tcaatataac
     6241 gaacacctgt tgttgctaaa tctgcgactt tctttccgcc tcggaaacga agcggtgtat
     6301 aaaattcttt ctcctccgaa agtttccctt cttcgatcat tcgctcgata tctgctaaat
     6361 actgtttcat agaagcatag gaaactttca catttggatg attcgtatag cccagcgcac
     6421 tgttgcgaat actgcgaaca ggttcttgcg gatgtgattc atctaaaaag tagttcttct
     6481 cactcattgg tgaagcaccg aataaataag tgatcatcca gcgatagcgc ataaagtttc
     6541 tcgctgtttt caaataaagt tctgttttga attcggaaaa atcttgaaat tcttcctgat
     6601 ggctgaacaa tgttcttagc aactcatcgc caaattcaaa attgaaatga ataccgctta
     6661 ccatctgttt tcgcttcccg tattcttttg ctaaataacg tcgatacaag acatcttcga
     6721 aattttttaa ttttgcaata atgatctctt cgtctttttc cggtaaggca ggtggcatac
     6781 ttaatggcca gatcatctct tctttcggta tagaacgagc agtcacatca taaacagcag
     6841 cgagatactg aaataattcg ggaatagaat cggtaacagg ggtgatcatc tctgtttgtg
     6901 tttcgctaaa atctgtttgg atataggggt gataggatcg atcgccaaag attgctggat
     6961 gatcggtttt tgctagattt cctgcaagat caacccgctg tccttcccgt tctataccaa
     7021 atcgagcttg gtcgataaaa gggcgcgcat tgacatgcaa taataattgc ttaaaattca
     7081 tcattaagtg acctccaatt catatgaatc taattataat caaaaagtct aagaaaaaga
     7141 acgaaattgt ttgagaaccg cttacaaata agtataaaaa tgaaggcatc aaaaacacag
     7201 ctatgttaaa aacaatcatt caaacaagga gctgcaactt ttgtcacagc tcctgctttt
     7261 tagacatggg ttattcttct tttagtaatc ctttgaacaa tccaacgaca aaatcatttg
     7321 gatcaaacgg ctccaagtca tcgattcctt caccaagacc gaccaatttc actggtaaat
     7381 gcaattcatt acggatagca agtacgatac cgccctttgc tgtaccatct aattttgtca
     7441 aaacaagacc ggtcacatct gttgtttctt tgaattgttt tgcttgaacc atcgcgtttt
     7501 gaccagtggt tgcatctaat acaagaagtg tttcttgggg tgcttctggt aattcccgct
     7561 ggattactcg ttttattttt tctaattcgt tcatcaagtt cactttattt tgcagtcggc
     7621 ctgcagtatc aactagtaaa acatctgcct gctctgtctt tgctctttcc aaagcatcga
     7681 atacgacagc tgctggatcg cctcctgcat tgccacgaac aacttcaaca ccagcgcgtt
     7741 cgccccagac aactaactgg tcgattgctc cagcgcggaa ggtatctgct gctgccatca
     7801 atactttttt accttcgttt ttgaactgat gggcaagttt accaatactt gttgttttcc
     7861 ctacaccatt aacaccgaca aacaaaataa ctgttagtcc gtcttcttgg atatttagtt
     7921 catttacttc attgattcct tccgcttcat agagatccac aagtttctcg atgatcgcat
     7981 tttggacttc tgccgatttt ttcgcatttc tgagtttcac ttcttgacgt aatgcttcgg
     8041 tgattcgtat cgctgtttca aaacctacat ctgcaccgat aagtgtttct tctaattctt
     8101 caaaaaagtc ttcatcgact gttcgaaaat tggcaaacaa ctcattcatc cgttcaccaa
     8161 atgttttacg cgtcttttct aaacccttgt cgtacttctc ctgtacttca acttcttctg
     8221 ttttctcttc gccagtaaaa gcacgcttga ttttatcaaa aaatcccatt tgactccccc
     8281 tactctaata caaatttttg gatcactttg gctacgccat tttcttcatt gctggctgtc
     8341 acgaaatctg ctgcttcttt gacgaaaggc acagcattct ccatcgctac gcctagtcca
     8401 gcatattgga tcatcggtaa atcattttcc tcatcaccga tcgccatgat ttcttcttgt
     8461 ttcaaaccaa gatcttttgc caataaagaa ataccataag cttttgtaac accttttggc
     8521 ataaattcca aaagcatact tcttgttttg atgatctcat acatttcatg gaattctgct
     8581 ggtatttctt tgattttagg atctaattct tcttggtcat aagcgattac tgccttatta
     8641 agcacaaatt catctgtaat atcgcttata tccgctggtt ggaattgcag cagcttattc
     8701 aacacattgt acaaggattg cttccttggg gcactaggca gctgtaaaac cacattgtca
     8761 gacaaaacat ctagtggcag atccaattgc tgagccagtt tgtataagcg ttggatatct
     8821 gatacttcca tcactttctt ttccatgatt tcaccagtat catttttttg taccaaaccg
     8881 ccgttgaaag tgatgctgta gtcaccttca tctaccagtc ctaattcttg cagataatgc
     8941 gccatcgcag ctaaggggcg tcctgtacat agaacgatct tgaccccttg ctgttttgct
     9001 tgtgctaatg cttgtttatt ctcatctgaa atcagctttt gttcatttaa taatgtaccg
     9061 tccaaatcga ttgctactaa tttgatcatt ttcgttccta cactttcgtt tcgttgattt
     9121 tcgcctcttc attgatatct tccatccgta cagaaacgat ttttgataca ccggattcct
     9181 gcatcgtcac tccgtacagt acatctgctg cgaccatcgt tcctttgcgg tgtgtgacta
     9241 cgataaattg tgtgtcatct tggaaatcgc tcaagtaacg accaaaacgt ttcacgttgg
     9301 cttcatctaa cgctgcttct acctcatcta agatacaaaa cggaacagga cagacacgga
     9361 taatggaaaa gagcaacgcg atggctgtaa gtgctcgttc tcctccagaa agcaaactta
     9421 gactttgcag ttttttgcca ggaggttgta cttcaatctc aatacctgtt ttcagcaaat
     9481 cagttggttc cgtcagtact aattcagctt taccgccgcc aaacatattt ggaaagacaa
     9541 ttttgaattc ttgacggata gcttcaaata cttctttgaa tcttgtccgc acttcatcat
     9601 ccatctcatc catcgtttca aacaattggt ttttcgctgc taacagatca tctcgctgtg
     9661 ttgctaagaa tgtatgtctt tcactgactt gttcgtattg ttcgattgaa tttaagttta
     9721 ctggtcccag tttttcaatc tgttctttta aggatgaaac ctttgttcta ctgtcttcga
     9781 tatcggttgt ttcttgatag tcttgggaag ctttctcaaa tgtcagctga tattctgact
     9841 gcagataggt caaatgatta tctaaaatca tttcagctcg gtctttttgg acttctaatt
     9901 tggattgttc cgacagtcgt gctttttgct cacgattacg ctctgccaag actgcttcaa
     9961 gcttatcgat ctccttttgc cttctatctc gctgctcttt ggcttttgcc aactcggctt
    10021 ttaatgtttc tcgctgagca gataattcgt taatttgttt ttccaaactt tcttctgtga
    10081 cttcatgatc agaaaagtct gctgtcaatg tcgccaactg cttttctatc gcttcttgac
    10141 gttcggttgc ctctgctttt tggactcggg caccgcggag ctggatctgc agatgattaa
    10201 actgctcttt caagacagca agatctgctt gttcttgtgc tttttgactt tgtacttgtg
    10261 ctcttctggc ttccatctgg tcgctttctt gacttaacga tttgatttct tcgtcgattt
    10321 tttgtcgttg cgattcaaga tctgtttgtt tgtctttcaa ttcgttcgtt tgcttttcgt
    10381 aatcttcgat aaactgctgc agttcacgtg tctcaaaagt tgagatttgc ttttcttttt
    10441 caaaacgttc aagttcattt atgatgtttt gtagctgatt tgtcgcttct tgttcttcaa
    10501 aacgtaattg ttcgcctcgt gtacgcaaaa cttcttgttc ttcggctaaa cgagctgttt
    10561 cttgttgcag ctcctgaact tttttttctt gtgcttggag ttgtttatct gcttcttcaa
    10621 attcactagt caactgtttc aattcttgat tttgtacaaa caaactgcct tggtttcctc
    10681 gtttattcgc tccacctgtc atggatcctc cggcattcat cacatcacct tctaaggaaa
    10741 ccactcgata ttgatagcgg atcgtttgcg cgattgcatt ggcactagtt aagtctttgg
    10801 ctaaaaggat cgttcctagc aggttttgaa ccacagtttg gatttgatcg ggataactga
    10861 cttgttcact ggcaatccct ataaaccctt ctacagctgc tgcttgtgtc aatatatgag
    10921 ccggtaattg tcttggtttg attgtcgtca gtggaaggaa tgttgctctt cctcctcttt
    10981 gctgtttcaa atacgtgatg gcttgccgag catctttttc attttcaacg atgacatgct
    11041 gggctgcccc acctaaagct gcttcgatag ctaacgtgaa atctgcagga acatcgatca
    11101 gttcagccac tgctccgaca atacccgaaa gctgctgttt atgctgcaag accaatcgaa
    11161 ccccttggta aaaaccaaaa tagttttctt ggatttcttg aaggcttttc ttacgagcac
    11221 gtagctgctg gacttgattc attaattgat acattttagg ctgttctttt gctaatgctt
    11281 cttggaactt tttcccgttt tgttggatca tttctaactt ctcttttgtt tcagttaaag
    11341 atgattttag ctgcgcctgc ttttgtgtca attcttcttt ttgtaagatc aatcggtcta
    11401 ctgatgcttc tacttccgat tgcttggcta acgtctgctt gctttttgct gtttcttgta
    11461 tatattgacg ttctagatac ttcagttcgt ttcctacagc tgcttgttct tgcatcaaat
    11521 caacatattg atctcttaat tctgccaata attctttcgt tgatttttgg tatttttcta
    11581 attcttgctg tgtttttatg agatttgctt cagctttttg gatctctgtt tctttttctg
    11641 ctgctgcttt catcaagctt tcttgaagct tttcaaaatg tttaaccttt ttttgtgctt
    11701 ctgctaatga agtttgatat tcttgcgagc ttttttgtgt atgctttgtc cgctcctgca
    11761 aaacatcttt ttgtccttct gtctgcttca gtttttccga aagatctaat aatacttgct
    11821 gatttttttc gatcagacga tcggcttgcg cattttcttt acgctgcttc gccaaaatgg
    11881 attcttgttc ttggatcgac tcagatagtt tgcctaattc tagattgaat ttcgcaagct
    11941 gtgcttgttt gttgtcccaa tctttttttg ctgtcttgat ttcagcaacc atcaaagaga
    12001 catcggtttg agtcagtgtt tcttttagcc gtaaaaactc ctttgctgcc tcactctggg
    12061 cggccaaagg agttaactgt tcttccaatt cgtggatgat gtcttggacg cggctgaggt
    12121 tgtcttctgt ttcaaaaagc ttctgttctg ctttcttttt ccgctgcttg tatttcaaga
    12181 caccagctgc ttcttcaaag atcccacgac ggtcttccgg tttactactg aaaatcgctt
    12241 ctacttttcc ttgagaaatg attgagaacg attcttttcc taatccagaa tctaagaaca
    12301 attcttggat atcttttaat ctgcagcttt gtttattgat gaaaaactcg ctttccccag
    12361 tccgtcgata acggcgggtt acactgatct cattgtactc caatggcaga taatgatcac
    12421 tattgtctaa aatcactgtg acttctgcaa tattcagctg cttacgggta tcggaaccgg
    12481 caaagatgat atccggcatt tttccgcctc gcagactttt agcagattgt tctcctaata
    12541 cccagcggat agcctctgta atattgcttt ttccgctgcc atttggtcca accactgctg
    12601 tgacgctatt ttcaaaatca atgactgtct tgtctgcaaa tgatttaaaa ccggctattt
    12661 caattcgttt caaatacaaa gctttccctc acttactttt acagtgctgc caatgcattt
    12721 tccgccgcag cttgttctgc taattttttc gatttccctt gtccagtgcc aatcaattga
    12781 tcatctacat aaacttcgat ccaaaatact cgttcatgag ctggaccttc ttcattgatc
    12841 aatcggtatt cgattgatac atcacctgat ttttgcaaga cttcttgtag ttttgtttta
    12901 tgatccatct catgtgaaaa agcacctgct ttgactttag gaaaaatcac tttttcgatg
    12961 aatgcatggg tcgtgtcgaa tccctggtca agatataacg cgcctaaaaa agcttcgaat
    13021 aggtcgcaaa gcaatgctgg tcgcgtacgt cctcctgaat tctcttctcc cttgcccaac
    13081 aaaacatatt gatcaaagtg acattctttg gcaaatttcg acaagctgtc ttcgcgaacg
    13141 atcgcagctc gtgtttttgt caattttcct tctggcaaat gaggatataa acggaataaa
    13201 tactgagaaa ccatcagttc taaaacggca tctcctaaaa attcaagtcg ctcattatcc
    13261 gataattgca gattgcgatg ctcattcaca taggatgaat gggtaaaagc ttgttcaagc
    13321 aaatttagat cgtgaaaaac gatgttatag ttttctttta gttcttttgt cagctggttg
    13381 tccattttcc acattccttt tatggatatt ttttcgatac ttccttatta tactggtttt
    13441 ttgtcaaatt tgaaatggtt cggtcaaaag gtaagtaaag ctttaataat cctagtgatt
    13501 gacgagaagt tgtgaaaaat tcgtttagtt tcgagtatga agaaaaaatt ttgtgaaaat
    13561 ggctttccat atttttgcat aaatttgact taataccgag aaactggact ttgaacaccg
    13621 tttattggag aagtcatgaa atcagcgcct tgacctgaat attagagaaa taaacagaaa
    13681 aatatcaaaa aaccgaaaaa ggacatcgcc ctttttcggt catttttagt gattatgaac
    13741 gtgaatctgc tgtaacagaa acatttgccc atgggtttgg tacatcataa gaccaatccc
    13801 agtctttgac acggtcgttg actggcaata cttcattacg gaacaacgtt ggaatcgcaa
    13861 atgcttcttc aaatgcatat tgttgccatt tttgatatgc ttctttttgg tactcttcat
    13921 caaatgcttt gcttgaactg atatcgttga tcaattttgt attttcttct gattcataac
    13981 gagtgtagtt gaacgcagca tttggtcccc atagtccact tggatttgga tcagttcctg
    14041 tattccaagc agcttggtaa acatcaattt caggatcatc gttttctaat ttatcataga
    14101 aagaattgaa ttcgatcaga cgacctgttg tgtattggac atccaaacca atttctttcc
    14161 attgttggat atagtaatct gccaatggtt gcgctgtttc cccaccggac atcgaagcaa
    14221 agttgattgt tagtttattt ccatctttgt cttctcttaa accatcgtca tccacatctt
    14281 tgtagccagc atcatctaat agttttttcg ctttgtctag atcttgtgtg aatcctttca
    14341 tctcatcatc atggtatgac tggaatactg gaggaatcaa tgaagtcgca ttgcttctca
    14401 aacctgcata gaaacgttca cctactgcat tattatctaa tgcatagccc attgcttgac
    14461 gtaatgattt atcagccatt tttgaatctg gattgtattc cacactcttt gtatctgcat
    14521 tccatttacc taacttgaat cctagataag tataagaagt ttgttcacgg cctaagtttg
    14581 tatagccagc aatttcttta taggtatcgt aagtatctgt tggcatttgg aaaatcatat
    14641 catataattt agagttcaat gcttcagttg cacttgccat cggtacagat ttcatcgtga
    14701 tctttttcaa tttaggcgta ccattccagt agtgctcgtt tggcaagtat tctactgatt
    14761 ctcctgcaac gatattactc atgtaataag ggccaaaagt cactggattt ttacgtactt
    14821 gatcgcttga ttccatatct ttgattgcta caccttctaa cgtatgtttt ggtgctgcat
    14881 atgaccagat accaccaccc gattgaagca tgcttgggtt tacttcttta tattcgattt
    14941 caactgtttt gtcgtctatt tttttgattc cggagattgt gtcggcactt ccatcatgat
    15001 attcttccat acctacgatg ttacggaaag tgtcgtcata acggatacca gtgtaatctt
    15061 tattcccaat gatttcgtaa gggaaaatca catcgtccgc cgttacatct tcaccatctg
    15121 accatttgac gttgtctttt actttgattg tcgctttttt cgtatcttca tctagatcaa
    15181 gcgttgcagc tccgtcgtct gtgattctga aatcgtcatc gtttgcaaat agcggttcat
    15241 gagaaggagc cataaaagct gcatcatatg aatcttggta aaactcccac tggaacaatc
    15301 ctttaaactg actgtccgtt gcgaccgcag cttctaattc gccgtcttcg attgcatcat
    15361 cattattttt tgttgcaatt ggaaaagtta attcctcttt gttattgccg ttgcctccag
    15421 agttgtttcc agaggtatct gtacctccgc ccccgcatgc tgcaagaacg acagcagaca
    15481 tcagcgctgc aaatccaaaa atcgttttct tcattcctat ttcccccttc gcttctatac
    15541 tagcctaacc gttggcgcgc atctgcagaa cgcttgaatg cttgtccaac ataattgata
    15601 ctcaacatca aaactaaaat aaggattgat gcgggtaacc agatccattg ccgattcact
    15661 aagacttctc cgttacttgc ataaccgatc aatgtaccta aactaggtac atttgccggc
    15721 aagccgaaac ctaagaaagt caaagttgtc tcgataccga tgtttgccgc aaagttcatc
    15781 gtcaaattag taatgattaa tgaacttaga ttcggcatga tttcgcggaa catgatttta
    15841 aaatcattgg ttcccattgt ttttgaggca ctgacataat ctcttcttgc ttcagataat
    15901 gtcttgcttc ggaataatcg ggctttagct acccaataaa atgcactcat gatgaatata
    15961 aaggaccaga cattgtatct ggaaataatc gatacgaaga cgataatgat cagcatgatc
    16021 ggtagaatca tgataaaatc aacgatccgc atcaagatgt tgtcaatgat ccctccgtag
    16081 tagccagaga tgatccctaa tccaaccccg atgatcgaag taaggatcgt gatcgcaaat
    16141 ccgatcgtaa cagagtttct agcacctatg atcaattgtc ctaatacatc acgtccgcct
    16201 tcatctgctc ccagaatgaa cttttgtcct tgtgtcgaaa ctgttcctgg tgcagcgtat
    16261 ttgtcgaaga tactaacata catcactttg tcttgatcgg tcagcatcgc accgataaag
    16321 atcactaata ataaaaccac tagcaaacct aatgaaaaaa tagcaatttt atctttttta
    16381 aattctcgag caatcattcg tatccccata ggaggaatac tttcttgaac tgctgtattg
    16441 tttttttcac tcatcgatcc attctccttt ctgattggtt ttattgaatc cgaatacgcg
    16501 ggtcaacgat actcatgatg atatctgaaa gtaatgtccc cactagcgtc gcgattccta
    16561 aaatcaatac taaggatgtg atcacggtat agtcccgttg gccgatggaa tcaataaata
    16621 gttttccgac tcccggataa ccaaaaatct tttcgataac tacagaacca gcaatcaacg
    16681 aagtgatttc ataccccatt tgagaagcca tcggcaacgc tgcatttcgg aaaatatgtc
    16741 gactgaatac tttattcgtc ggtacccctt tggaacgggc tgttctgaca aaatcgagcg
    16801 atttcgaatc gattacttca cttcgcagat attggattgt gacggcagtc gccaaaaatg
    16861 cttgggtaat tgatggtaac agaatatatt tcaaacggtc tatccattga gtgataaatc
    16921 caccttgcaa acctagtgtt tgtgatccac ttgttgggaa ccagcctaaa cgataaccaa
    16981 aaacaaatag catgatcaag ccgaagataa ataaaggaac agcaaaacta aagaagttgt
    17041 agattacgac aatcttatcc aagattgaat tttgataccg gccagcaagc atccccagag
    17101 gcacagcaat caaataagta aggacaactg tcaagatcga tagatataaa gtgttgatga
    17161 tccgttcgcc gatcaagtca gcaacagagc gtttgtataa gaaactttga ccaaaatcgc
    17221 cttgcaaagc atttccgatc caacggaagt actggacata ccatgggtca ttcaagccag
    17281 ctgcttgtcg catctgttcg atcactgccg gatcctgatt tggattgatc aaaccggtaa
    17341 atggatcccc cggcatcgct tgggctaata agaagatcag aacacttaaa ataataactt
    17401 gcgggatcat caataaaaca cgtcgcagaa tcgttttcca cattagcttg cacctccatc
    17461 tttcaacgcc actttgtgtg tagttgtgat cgtacgcaaa tcgtagactc gaccagtcgc
    17521 atcatagtat tctttttggt tttggatata ctcttgttct actcttcgtc gattttcttt
    17581 gtggctttcc ctattttcta catcgatttg cggaatagct gaaagcagac gtttcgtata
    17641 gatatgtcgc gggtcattat aaatgtcttc tcttgtaccg atttctacaa atcgaccttt
    17701 gtacataatt gcgatattgt cgcacatgtg ttttactaca cctaaatcat gtgaaatgaa
    17761 taaatagctg aggccgtatt cttgctggat tcgtttcatg aaattcaata cttgcgcttg
    17821 aacagataga tccagagcag atacaggttc atcggctacg atcagttttg gattggtagc
    17881 aacagcacgg gctactccta gacgttgacg ttgtccgcct gaaaattcat gaggatattt
    17941 atatagcgca tcttccggca tcccaacgat atctagcagt ccttttacct ttttcttttc
    18001 ttcttgatcg cttaaccgtt caaaatttcg gatgggttca gcaatgatat ccaacactcg
    18061 ctttttagga ttcaaactgg acattgaatc ttggaagatc atctgcacat ccttgttata
    18121 acccattttt ttgcggttgc ttcgcttcgt tacatctttt ccttcataca tgatttgtcc
    18181 ggctgttacc ttttctaatc ccacaacggc ttttcccgta gtagattttc cagagccaga
    18241 ctctcccact aaaccatatg tctttccttt ttcgataata aaatccacac catctacagc
    18301 atatacatga tccgttactc gattgaagaa tccactgcga atagggtaat gaactttcag
    18361 gtctttgatt gtgattaatt ctgtcatcta cacctctcct ttttcgtctc taaagtggaa
    18421 atgcttatag caggagcaac gtaccaagtg gttcggtgcg acttcatgaa ggataggctc
    18481 ttcttcatga tcgcttgctg gtatccatgg aatacgtggt gcaaaccgac aacctttacg
    18541 aggcatgttt ttcagagaag gaacgacacc ttcgatcaca tggagttctg catcatgatt
    18601 tccttcttgt ggtatagagt tcagtaaaga gcgtgtataa ggatgcttag gatgatcaaa
    18661 cagttctttt acacctgcta cttcaacgaa ctgtccgcca tacatcactg ccactcgatc
    18721 agccatttct gctacgactc ctaagtcatg agtgatcaag atgatccctg tttttgtttc
    18781 ttcctgaagg tctttcagta aatcaagaat ctgtgcttgg atggttacat ctagggctgt
    18841 cgtcggttca tctgcaatga cgattggagg tttgcaggcg attgcgattg cgatgatgac
    18901 acgttgtctc ataccgcctg atagttcatg gggatattgg cgtccaactc tcgcaggatt
    18961 aggaatccct acttgttcta gcaattctag tgcacgagct tgacgctgtt ctgcattcat
    19021 ctttgtgtga taggaaagtc cttctttgat ctggtcttcg attctcatga gaggatttaa
    19081 tgcagacaac ggatcttgga aaatcattcc gatatcattt ccgcggattt tattatagag
    19141 cgtttcgttc aggtcgatta aattcagatc gttatataaa atctcacctg taatttttgt
    19201 gttattagga tcaagtaatc ccataatcgt agtagccaag gtacttttcc cgcacccaga
    19261 ttccccaacg attgctaaaa tctcgttttt gtctaaagta aatgaaacgt catctactgc
    19321 atcgtaatag tcatctttga gtcggaatcc tgtatgtagg ttctgaactt ctaataattg
    19381 gttatcggac aatcgaatac ccctctctgc ttcctaaatg gaagtttttc ttaataaaaa
    19441 aggacattac acagaataaa tcgttttata acaaaggtta ttcttatctt tgtgtaatga
    19501 attattaata attatataga aaaccgcttt actttgcaac ataatttctg tttatcgcca
    19561 gaattatcag aaaaatgact tttacgtgtc aaaaagatta aa
//
LOCUS       DAZKPO010000062        17424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000062 DAZKPO010000000
VERSION     DAZKPO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.062"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..666)
                     /locus_tag="ACG8NZ_002034"
     CDS             complement(121..666)
                     /locus_tag="ACG8NZ_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0539692.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(678..1370)
                     /locus_tag="ACG8NZ_002035"
     CDS             complement(678..1370)
                     /locus_tag="ACG8NZ_002035"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0539693.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1389..1673)
                     /gene="macP"
                     /locus_tag="ACG8NZ_002036"
     CDS             complement(1389..1673)
                     /gene="macP"
                     /locus_tag="ACG8NZ_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0539694.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1686..2249)
                     /locus_tag="ACG8NZ_002037"
     CDS             complement(1686..2249)
                     /locus_tag="ACG8NZ_002037"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0539695.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2477..3139
                     /locus_tag="ACG8NZ_002038"
     CDS             2477..3139
                     /locus_tag="ACG8NZ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0539696.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3263..4459
                     /locus_tag="ACG8NZ_002039"
     CDS             3263..4459
                     /locus_tag="ACG8NZ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0539697.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4560..5936)
                     /locus_tag="ACG8NZ_002040"
     CDS             complement(4560..5936)
                     /locus_tag="ACG8NZ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0539698.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5973..6839)
                     /locus_tag="ACG8NZ_002041"
     CDS             complement(5973..6839)
                     /locus_tag="ACG8NZ_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0539699.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6928..7953)
                     /locus_tag="ACG8NZ_002042"
     CDS             complement(6928..7953)
                     /locus_tag="ACG8NZ_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0539700.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7971..8645)
                     /locus_tag="ACG8NZ_002043"
     CDS             complement(7971..8645)
                     /locus_tag="ACG8NZ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0539701.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8829..9722)
                     /locus_tag="ACG8NZ_002044"
     CDS             complement(8829..9722)
                     /locus_tag="ACG8NZ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0539702.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9796..11493)
                     /locus_tag="ACG8NZ_002045"
     CDS             complement(9796..11493)
                     /locus_tag="ACG8NZ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0539703.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11486..12799)
                     /locus_tag="ACG8NZ_002046"
     CDS             complement(11486..12799)
                     /locus_tag="ACG8NZ_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0539704.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12799..16740)
                     /locus_tag="ACG8NZ_002047"
     CDS             complement(12799..16740)
                     /locus_tag="ACG8NZ_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0539705.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16849..17307)
                     /locus_tag="ACG8NZ_002048"
     CDS             complement(16849..17307)
                     /locus_tag="ACG8NZ_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0539706.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4892 a         3904 c         3025 g         5603 t
ORIGIN      
        1 cggaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa caaagtcgtt
       61 tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca ctttgttttt
      121 ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt catgtccaac
      181 gggatcgaaa cttgccacgg aatctttgaa aatatggagt ttgtatccta gattatacgc
      241 atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat aaatatctgt
      301 gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg aataatgccg
      361 tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac tttttaaaga
      421 gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata agcggttctc
      481 cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa aatccttttg
      541 atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc ctgctgtccc
      601 agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc
      661 gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat ttctgctgat
      721 cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg atctgctgtg
      781 tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt ttgtccaaca
      841 gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat ttctttgatt
      901 ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt tgcgttcaaa
      961 ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc ttcataatat
     1021 agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa
     1081 taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc gattccgcca
     1141 gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag acttgtggta
     1201 atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg gttacctaat
     1261 gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct taggttctct
     1321 ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat ctttttaatt
     1381 ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag gataacaata
     1441 ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc gctgatcctt
     1501 gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc aatctttttt
     1561 tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc gcttgcttgt
     1621 gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc cataagccga
     1681 tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat cgtctttgca
     1741 tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag atgatacaac
     1801 tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac cccttgtgca
     1861 tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga agtaagatgg
     1921 ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc tgtcatttcg
     1981 ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat cactttttct
     2041 aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa agcaatgatc
     2101 ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc tggcaaacga
     2161 acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg actgattgtt
     2221 ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt aatcataact
     2281 gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc atagataaac
     2341 cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt ctagtagtcc
     2401 gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca acgataaatg
     2461 acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat tggtcttgct
     2521 atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat tgataggtgt
     2581 agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac aagaagaaat
     2641 tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga cagcgaatga
     2701 aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc aattgcaaat
     2761 gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc cagtcaaagc
     2821 agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg aagcaagtca
     2881 gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata atgagatcaa
     2941 tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg cgcagatcat
     3001 cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga
     3061 tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata ataaatatga
     3121 accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca ttcagatgat
     3181 gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa tcgagaacag
     3241 attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag atgttacacc
     3301 agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa cagatacgct
     3361 gaccacttca caacaagcgg agattgatca gctccaagag caacagacag ctgctcgttt
     3421 aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag caaaaatcga
     3481 tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa aattaagtga
     3541 attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg taggagattc
     3601 cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc gtgccggaga
     3661 agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc
     3721 aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag ataaagaaaa
     3781 agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa acaaagacta
     3841 ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag aacttcaaac
     3901 aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga tggatgttca
     3961 gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc atgatcttcg
     4021 cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc aaaacacaaa
     4081 tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc tgtggaaaaa
     4141 ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca
     4201 agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga aaatctcagc
     4261 catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac
     4321 tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag aaggaaaaga
     4381 aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga aacaaaaact
     4441 gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc ccaacaacag
     4501 tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc cattggagtc
     4561 tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat cagtccaata
     4621 accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat caatacaata
     4681 caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa ttctccagct
     4741 tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag catcaaatag
     4801 gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa tccggcaaag
     4861 aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg cttttgatcg
     4921 atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag taaacgagca
     4981 agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc tacagcgatc
     5041 accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg gtcattgact
     5101 cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat gtaaaggaca
     5161 gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg gtttttgact
     5221 tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac aagagctgca
     5281 ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc aggtttaacg
     5341 aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata taccatggca
     5401 ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac gaatatcact
     5461 acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc gccagttaat
     5521 gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc agaccatccg
     5581 gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc tgtcagccag
     5641 gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat cactggcact
     5701 cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc agctaagaca
     5761 aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt caccacaaat
     5821 attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt ttttagttct
     5881 tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa atccattgaa
     5941 gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata ccctaaaagg
     6001 ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc ttttttcgtt
     6061 tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc catttgtgaa
     6121 agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca gatgtttcct
     6181 aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac tttaacttta
     6241 cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa gataattgac
     6301 atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc aatcgtagag
     6361 aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt atctattttt
     6421 ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc taaagcaata
     6481 ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc
     6541 gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac acccatgtac
     6601 ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga taaaaatccg
     6661 atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac taaagaaaac
     6721 agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc aattttacca
     6781 cttactaaac caatactacc ccatgccaac atagggacta atgctattaa aatttccata
     6841 accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt aacaacacaa
     6901 gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat ttaagattgg
     6961 gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca aatggatacc
     7021 taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat gtgttttgct
     7081 aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt cttccgctgg
     7141 gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct tttctatgct
     7201 cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca attggttgaa
     7261 gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa cgatctcgta
     7321 gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct gaatgattcc
     7381 ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata
     7441 caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta atgtatactt
     7501 ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg aaagtggcac
     7561 tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat tactgattgc
     7621 tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt ctaccagttt
     7681 ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt tttgtatgtc
     7741 ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat aaaaaatcag
     7801 aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt tgatgtttgg
     7861 cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca agatcaatgt
     7921 ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt tcagggaacg
     7981 atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg ttcgattttt
     8041 cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc
     8101 cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt catcaaaata
     8161 aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat ttccatactt
     8221 ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga taattctgtg
     8281 tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc gatttggaaa
     8341 tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc catattcgta
     8401 ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt ttgacaccaa
     8461 taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg ttcttctctt
     8521 tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc
     8581 tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac catgattttt
     8641 gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa gcccttgtga
     8701 aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat
     8761 gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta ttcttaataa
     8821 ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt tcgataacac
     8881 caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag taatcgataa
     8941 agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa gggaactcgt
     9001 cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg
     9061 actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag gacaacaatt
     9121 ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa tcggtcatct
     9181 tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct atgggcggta
     9241 atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag gactgtcgct
     9301 cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac attgattttc
     9361 ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg attttggaca
     9421 tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat tactgatgta
     9481 tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg tttttcttct
     9541 ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa ataattgctg
     9601 agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa catcactgcc
     9661 ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat tttctttttc
     9721 attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc gattgctgca
     9781 gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc agctgtacgc
     9841 tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc caccagtagc
     9901 aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt ctactcctac
     9961 gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa aggctgcctc
    10021 acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat aggttccata
    10081 atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg ttgcttgtcc
    10141 gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag gcgactcccc
    10201 tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc ttgcatcttt
    10261 aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg ttgtgacttt
    10321 ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga tttcaataac
    10381 cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca aattatacgt
    10441 gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta ctttggctac
    10501 tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt ctactatata
    10561 atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct gatcataata
    10621 agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag cgcttccgtt
    10681 ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag aagccgtttt
    10741 tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat taataatcgt
    10801 tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc tgatttcgtt
    10861 tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac catcccacac
    10921 gccatccacc atatcaggca tcatcactgg catcataaca accattggag acgcgattct
    10981 ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc tagaagaagg
    11041 tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat tcgttgtttg
    11101 tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc gtttttgaat
    11161 ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta aaggattatc
    11221 tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat
    11281 attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt ccggaacaga
    11341 tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg cgacttcttc
    11401 ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga gtaacagtac
    11461 actcattccc gtgacaatca tttttttatt catttttttc tccccgttct ttttttatta
    11521 ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat
    11581 tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga ccactcggct
    11641 ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt gttgcggatt
    11701 cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt ccgttaacta
    11761 ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg acctcgattc
    11821 tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca aaataatagg
    11881 tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt
    11941 tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat tgatttttca
    12001 agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc tgatatagag
    12061 caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga cgtccatact
    12121 tgaagaagta tggatctctt agataaccga catttttagg atagatatga atctcttgca
    12181 gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt gggatagctg
    12241 tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg aaaatcaaat
    12301 aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc ccgtctccta
    12361 atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca agcctttctt
    12421 ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg acgaattctt
    12481 taggagtcat gttttcagca gctttttttt catgataata atcggtcatg tcgtaaatgg
    12541 caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt
    12601 cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta taatcgatat
    12661 ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat tcttctgccg
    12721 acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat tgaagcatca
    12781 aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga caaacaaacc
    12841 ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga ataatcgtcc
    12901 ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa gctgtaatct
    12961 gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag gcgtctctgc
    13021 ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt ttcctggaac
    13081 tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa taaagtaacc
    13141 tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga ccccttccaa
    13201 caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc ctccctcgat
    13261 gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc cttcaagacg
    13321 tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta gttcatcttc
    13381 taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg gagcagtttc
    13441 gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg caaactggaa
    13501 cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct catattcacc
    13561 aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta ctcctacggc
    13621 acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt ctaacggatt
    13681 accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga tatcgaaacc
    13741 tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct cccgataatc
    13801 aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg ttgtatacgt
    13861 gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta tagcattatc
    13921 gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt ctgggttctg
    13981 gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct tataaacaaa
    14041 tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta catatttccc
    14101 gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt cgttcacacc
    14161 agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg tttcataaag
    14221 ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat tgatattcat
    14281 gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat catactctgt
    14341 gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt cataatagga
    14401 attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt catctacaag
    14461 ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt ggaattgata
    14521 cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc
    14581 gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc
    14641 gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg gattttggtc
    14701 atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct tgctgacttg
    14761 gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca tccgtccagt
    14821 agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt tcactgtgca
    14881 aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc tgaccatttc
    14941 cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc cgacatacgt
    15001 tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat cccatatttc
    15061 tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact gaatatctgc
    15121 cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg
    15181 ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa tgtcattctt
    15241 gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag caatattttt
    15301 caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc catcgattcc
    15361 taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca gccaatgatg
    15421 ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc cattcgtatt
    15481 gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt cttttacctc
    15541 tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag atgcataggt
    15601 aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa aatttctgaa
    15661 caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc tccaagctcc
    15721 attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc
    15781 tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt ctttatacaa
    15841 tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg gtgtacgatt
    15901 taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc
    15961 cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat tcaccatacc
    16021 gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt ttactgcctc
    16081 aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac tcatactcgc
    16141 tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct cgcccccgat
    16201 gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat cagatgctgt
    16261 gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat aatccaagta
    16321 actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg gaacgtttct
    16381 atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc gccagttcgc
    16441 tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg gcgagccttt
    16501 gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg tatagtcatt
    16561 cgttgtaaac ttcgggtagc gatgctgttc gttttcaact tctccgctag gatagatata
    16621 ttcatagggg tcttgcgtag ctgttggccc aagacgctcc aaaataccgt tgaataactg
    16681 gcgttgaatg cgctggagtg cttgaccgct gggtacgatt ggttcgtcca gcttttccat
    16741 ctcattttct tcgctacgtt gaatttcagc ggaatcttct gtatcagttt cattttcttg
    16801 taattcttgg ttatctattc tttcatcaac caccacttga agaggtagtt acccttcgtt
    16861 cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt cttttgaata
    16921 aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt cttctccatc
    16981 ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa tcccggcggc
    17041 tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt tttcaaaagc
    17101 cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt ttgcatagtc
    17161 ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta catccagggt
    17221 cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag ttactaactg
    17281 caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat atttgtcttc
    17341 tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa ttttaatttt
    17401 aaatcaaatg atatcctttt tagt
//
LOCUS       DAZKPO010000063        16831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000063 DAZKPO010000000
VERSION     DAZKPO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.064"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..386)
                     /locus_tag="ACG8NZ_002049"
     CDS             complement(<1..386)
                     /locus_tag="ACG8NZ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539707.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKIL"
     gene            complement(571..969)
                     /locus_tag="ACG8NZ_002050"
     CDS             complement(571..969)
                     /locus_tag="ACG8NZ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0539708.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(966..1739)
                     /locus_tag="ACG8NZ_002051"
     CDS             complement(966..1739)
                     /locus_tag="ACG8NZ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0539709.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1736..2419)
                     /locus_tag="ACG8NZ_002052"
     CDS             complement(1736..2419)
                     /locus_tag="ACG8NZ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0539710.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2586..3473)
                     /locus_tag="ACG8NZ_002053"
     CDS             complement(2586..3473)
                     /locus_tag="ACG8NZ_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539711.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3470..4339)
                     /locus_tag="ACG8NZ_002054"
     CDS             complement(3470..4339)
                     /locus_tag="ACG8NZ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0539712.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4476..5762)
                     /locus_tag="ACG8NZ_002055"
     CDS             complement(4476..5762)
                     /locus_tag="ACG8NZ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0539713.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6035..6613
                     /locus_tag="ACG8NZ_002056"
     CDS             6035..6613
                     /locus_tag="ACG8NZ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0539714.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6613..7542
                     /locus_tag="ACG8NZ_002057"
     CDS             6613..7542
                     /locus_tag="ACG8NZ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539715.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7539..9275
                     /locus_tag="ACG8NZ_002058"
     CDS             7539..9275
                     /locus_tag="ACG8NZ_002058"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0539716.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="ACG8NZ_002059"
     CDS             complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="ACG8NZ_002059"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0539717.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10171..10680)
                     /locus_tag="ACG8NZ_002060"
     CDS             complement(10171..10680)
                     /locus_tag="ACG8NZ_002060"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0539718.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="ACG8NZ_002061"
     CDS             complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="ACG8NZ_002061"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0539719.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11981..13072)
                     /locus_tag="ACG8NZ_002062"
     CDS             complement(11981..13072)
                     /locus_tag="ACG8NZ_002062"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0539720.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="ACG8NZ_002063"
     CDS             complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="ACG8NZ_002063"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0539721.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="ACG8NZ_002064"
     CDS             complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="ACG8NZ_002064"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0539722.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="ACG8NZ_002065"
     CDS             complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="ACG8NZ_002065"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0539723.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4874 a         3711 c         2975 g         5271 t
ORIGIN      
        1 agaatctttt gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc
       61 atttgttttt gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt
      121 aaaccttctg gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata
      181 taatctttta ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct
      241 tctgcttgat agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc
      301 agcaatgttt tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta
      361 tcttttaaaa caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag
      421 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
      481 taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      541 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      601 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      661 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      721 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      781 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      841 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      901 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      961 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
     1021 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
     1081 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
     1141 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
     1201 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
     1261 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
     1321 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
     1381 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1441 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1501 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1561 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1621 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1681 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1741 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1801 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1861 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1921 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1981 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     2041 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     2101 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     2161 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     2221 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     2281 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     2341 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     2401 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2461 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2521 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2581 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2641 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2701 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2761 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2821 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2881 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2941 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     3001 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     3061 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     3121 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     3181 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     3241 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     3301 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     3361 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3421 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3481 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3541 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3601 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3661 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3721 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3781 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3841 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3901 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3961 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     4021 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     4081 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     4141 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     4201 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     4261 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     4321 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     4381 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4441 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4501 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4561 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4621 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4681 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4741 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4801 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4861 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4921 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4981 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     5041 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     5101 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     5161 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     5221 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     5281 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     5341 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     5401 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5461 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5521 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5581 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5641 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5701 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5761 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5821 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5881 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5941 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     6001 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     6061 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     6121 tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc
     6181 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     6241 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     6301 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     6361 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6421 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6481 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6541 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6601 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6661 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6721 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6781 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6841 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6901 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6961 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     7021 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     7081 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     7141 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     7201 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     7261 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     7321 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     7381 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7441 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7501 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7561 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7621 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7681 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7741 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7801 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7861 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7921 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7981 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     8041 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     8101 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     8161 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     8221 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     8281 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     8341 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     8401 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8461 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8521 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8581 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8641 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8701 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8761 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8821 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8881 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8941 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     9001 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     9061 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     9121 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     9181 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     9241 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     9301 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     9361 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9421 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9481 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9541 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9601 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9661 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9721 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9781 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9841 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9901 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9961 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
    10021 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
    10081 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
    10141 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
    10201 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
    10261 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
    10321 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
    10381 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10441 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10501 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10561 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10621 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10681 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10741 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10801 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10861 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10921 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10981 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    11041 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    11101 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    11161 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    11221 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    11281 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    11341 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    11401 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11461 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11521 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11581 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11641 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11701 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11761 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11821 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11881 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11941 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    12001 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    12061 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    12121 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    12181 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    12241 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    12301 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    12361 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12421 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12481 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12541 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12601 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12661 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12721 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12781 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12841 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12901 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12961 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    13021 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    13081 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    13141 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    13201 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    13261 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    13321 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    13381 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13441 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13501 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13561 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13621 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13681 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13741 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13801 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13861 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13921 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13981 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    14041 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    14101 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    14161 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    14221 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    14281 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    14341 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    14401 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14461 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14521 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14581 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14641 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14701 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14761 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14821 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14881 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14941 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    15001 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    15061 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    15121 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    15181 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    15241 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    15301 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    15361 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15421 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15481 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15541 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15601 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15661 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15721 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15781 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15841 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15901 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15961 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    16021 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    16081 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    16141 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    16201 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    16261 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    16321 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    16381 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16441 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16501 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16561 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16621 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16681 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16741 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16801 agaattgtac ctagtataga cataaaaaaa t
//
LOCUS       DAZKPO010000064        16502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000064 DAZKPO010000000
VERSION     DAZKPO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.065"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8NZ_002066"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8NZ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0539724.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8NZ_002067"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8NZ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0539725.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8NZ_002068"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8NZ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0539726.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8NZ_002069"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8NZ_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539727.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8NZ_002070"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8NZ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0539728.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8NZ_002071"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8NZ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0539729.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8NZ_002072"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8NZ_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539730.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8NZ_002073"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8NZ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0539731.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8NZ_002074"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8NZ_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0539732.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8NZ_002075"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8NZ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0539733.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8NZ_002076"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8NZ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0539734.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8NZ_002077"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8NZ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0539735.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8NZ_002078"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8NZ_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0539736.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8NZ_002079"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8NZ_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0539737.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8NZ_002080"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8NZ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0539738.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8NZ_002081"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8NZ_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0539739.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8NZ_002082"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8NZ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539740.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8NZ_002083"
     CDS             13967..14695
                     /locus_tag="ACG8NZ_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0539741.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8NZ_002084"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8NZ_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0539742.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8NZ_002085"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8NZ_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539743.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8NZ_002086"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8NZ_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539744.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8NZ_002087"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8NZ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0539745.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8NZ_002088"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8NZ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539746.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4645 a         3475 c         2559 g         5823 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 ag
//
LOCUS       DAZKPO010000065        16211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000065 DAZKPO010000000
VERSION     DAZKPO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.066"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1570)
                     /locus_tag="ACG8NZ_002089"
     CDS             complement(<1..1570)
                     /locus_tag="ACG8NZ_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0539747.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTII"
     gene            complement(1557..3245)
                     /locus_tag="ACG8NZ_002090"
     CDS             complement(1557..3245)
                     /locus_tag="ACG8NZ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539748.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3391..5028
                     /locus_tag="ACG8NZ_002091"
     CDS             3391..5028
                     /locus_tag="ACG8NZ_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539749.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(5173..5913)
                     /gene="truA"
                     /locus_tag="ACG8NZ_002092"
     CDS             complement(5173..5913)
                     /gene="truA"
                     /locus_tag="ACG8NZ_002092"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0539750.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6104..6739)
                     /locus_tag="ACG8NZ_002093"
     CDS             complement(6104..6739)
                     /locus_tag="ACG8NZ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0539751.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6886..8106)
                     /gene="sstT"
                     /locus_tag="ACG8NZ_002094"
     CDS             complement(6886..8106)
                     /gene="sstT"
                     /locus_tag="ACG8NZ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0539752.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            8325..9323
                     /locus_tag="ACG8NZ_002095"
     CDS             8325..9323
                     /locus_tag="ACG8NZ_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539753.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9519..11354
                     /locus_tag="ACG8NZ_002096"
     CDS             9519..11354
                     /locus_tag="ACG8NZ_002096"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0539754.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(11414..12130)
                     /locus_tag="ACG8NZ_002097"
     CDS             complement(11414..12130)
                     /locus_tag="ACG8NZ_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0539755.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(12357..12899)
                     /locus_tag="ACG8NZ_002098"
     CDS             complement(12357..12899)
                     /locus_tag="ACG8NZ_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0539756.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12904..13131)
                     /locus_tag="ACG8NZ_002099"
     CDS             complement(12904..13131)
                     /locus_tag="ACG8NZ_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0539757.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(13210..15090)
                     /locus_tag="ACG8NZ_002100"
     CDS             complement(13210..15090)
                     /locus_tag="ACG8NZ_002100"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0539758.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(15279..15893)
                     /locus_tag="ACG8NZ_002101"
     CDS             complement(15279..15893)
                     /locus_tag="ACG8NZ_002101"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539759.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            16035..>16211
                     /locus_tag="ACG8NZ_002102"
     CDS             16035..>16211
                     /locus_tag="ACG8NZ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0539760.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         5163 a         3065 c         2528 g         5455 t
ORIGIN      
        1 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
       61 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
      121 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
      181 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
      241 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
      301 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
      361 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
      421 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
      481 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
      541 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
      601 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
      661 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
      721 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
      781 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
      841 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
      901 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
      961 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
     1021 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
     1081 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
     1141 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
     1201 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
     1261 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
     1321 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
     1381 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
     1441 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
     1501 agatgaaaga atatatacta ctaactataa ttaatccact caatattact acttttttat
     1561 ttattttcat tcttcttgtt tatctccttt ttagtcacat atcgagaatc tttcaactta
     1621 ttaggtgcaa atcgtttaaa acttgaatac accactaaac aataagtcag aatactgatc
     1681 cataaagcca tgagagcagt tggagtatat tttgttgtaa tttctatctt attttctcca
     1741 gcatttacat ccacattcac taagccatgt tttttataag agtcatttac gactttaccg
     1801 tttactttta cctgtgtacc taaataagca tatactggta ctgtcacttt ttgctttttg
     1861 ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt tatacttccc tttttcccac
     1921 gattctccta cttttacttc atgtttttct aagtgaggcc tttcttttat cgcttctgtt
     1981 ggggcgtaat cgtataatcc tcccatatct gatgttgtat aacgtgggag tgtttcttca
     2041 ttgataacaa tatgaccgtg ttttgattgg ataacttttg cgtaattttc ttttgctgct
     2101 atagacgtga cgagtagaaa caccacagta gcgccaatga ttaaccatga atatttttta
     2161 ttcatagtct ttttcataaa aacaagcgtt atgatcattg aagaaaaaaa gatttggata
     2221 cctaagatac gccaaggaaa ctggagtaag cttatcggtg ttttttgaaa caaaaaccat
     2281 ggaaataaat tagttacgca tacccatgta gaaactacgc caacatagat gattttttct
     2341 tttttctcca tttctttcag tatgaataaa ggaaaaatca acgcaaccaa tcctaataac
     2401 cccatagtgt atgttgtaat atcgttattt aacgcttgaa tgatactatc tgaaatagaa
     2461 agtgcttcta gctgcaaatc tgttttgaag ggactgtgta tttcttgata attcatctgc
     2521 tgcaacatag gataccaaaa atagctagtt aatgcacaag tcaaaaaaac agcctttact
     2581 aaattcacga ccacaaattt tatatcgttt tttatttgct tgcttaaaaa caaacgaact
     2641 ataatcatac aagcaatcaa tgcactacac aatagtgtag ttaagacatg agtatacaca
     2701 agtagactta tactgacaga taaagcgatg actttttttt ctctattgaa tatcaatcca
     2761 taagcataat ataaaacagc tgggataatc gtaagagcga ttgcttctgc aattgcggat
     2821 cgataataga tatcaactgt tcgataagtt gcaaaagtat ataaacaact aaataaaaaa
     2881 gcagcatcta tctttttaaa aaacttttct ccataataaa aactaatcag aatcgtacaa
     2941 atatttaata accaaatata tagaacataa cttaatatta agttatttga aattccataa
     3001 aaaacgactg caggaattat tgtaagaaaa ggataaaaat agttcagtcc atctccataa
     3061 ttgttaaatg ttgtatagtt gattggccct tcaaaaatag aagataaccc tttgattcgt
     3121 gaaagatgaa aagataaatc acctgttgta taaatttctt gagcatttct cataaaattt
     3181 actgaatata aagtactaag taagataaga actactaaaa gaaatgcttt tttacttatt
     3241 ttcatagatt tgttgttctc cttttttaat tctattttca gtatactaga attgtctttt
     3301 tttaataagt tttgcgttaa atttttcata gcactagtat gatatactag aaaagaatca
     3361 atttttatta cttttaaata ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat
     3421 atcattttta tgttatttct aagttttgct tacgtcatcc ctctattcat gtctaaggga
     3481 atctatcata gtgtcaatca ggatacttac tttcatttat ctcgaatcat aggactagat
     3541 aatgtatgga gttctccagt aaacttcaat aacttcgatc atcatggaac aatgatgaat
     3601 attttttatc catggcttac tctttatcca gcttttttat tttataaaat gatgggaaat
     3661 cttgttttaa gttataatat ctactatttt tttatcactt ttttaacaat ggtcgtttct
     3721 tatttttcta tgaaacaaat aaaaaacaat cgttatattt cgctgttatt ttcgattatt
     3781 tatacatttt ccgcctatcg tgccattgac atattccatc gtgcttcatt gggagaagct
     3841 gtcgctttaa catttttgcc tttgatacta atggggtgtt acgaaattta tattagagat
     3901 taccaaaaat ggtactggtt gtctattggc atgacgctag tagtgtatac tcatttactt
     3961 tctgtcgcta tggtttctgt atttattgga ggaacgttgt ttctttcttt ttatttttgg
     4021 gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta ccgtcctcac ctttttttta
     4081 agtgcgggat tcttaattcc ttttattcaa caatctagag cacaagaatt gaaagtccca
     4141 ctgggaaaag aattaagtgg tatggctcct agtgatatgc tgacacatat tttaaataat
     4201 aactataaca actatactat tggattattc ttatttctcg gattgattgg tgcttttatt
     4261 tttataaaaa aattaactgc tgatgattta tttattttct ttcttggtgt atttgttctt
     4321 ttttgttcaa ccaatttatt tccttggcaa ctttttaacc atacaccagt taagagtcta
     4381 caatttgtct ggcggttaaa tggtttttct tctttattta tagcctatac gatgagtata
     4441 gtgatctatt atattttttc tactaaaaat aattcatgga aaataatggt ctttacgttt
     4501 cttgcactaa tattgcatct ctcaggagta agcaattcta tccatgctaa caaagattca
     4561 ttaacactta tagcacctga agacgctgtt gctatagctg aaaattacaa tcatactgat
     4621 tatgcaaaca aagagtcaat ctatcatcct gatatgatta ataatgatca atatttacta
     4681 ggtaaccagg aaataaatcc aacaactcat tttatgagta acaaacttac aattgaatta
     4741 gataattcga ataataaaaa tactgtcttg acgacaccta tttatcgtta taaagggcaa
     4801 gtggcttcta ttaatggcaa actagtacaa acaaaattat ctaagtttgg cacaactgaa
     4861 ttaactattc ctccaggtat aaataaagtc gttattactt atcagtacac aaaactagca
     4921 attgcttcaa gatatttatc tatcgttact ttgattttat ttttacttta tcgatttact
     4981 ttttctaaac aaaaacaacc gagaagagaa gtacaacatt ctcactagat ttttatttaa
     5041 gaaagacttt ttagctgtac acataaacag gctaaaaagt ctttttttat atttctttaa
     5101 tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt ttcatccatt
     5161 atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg cagggacggt
     5221 ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt ctatcggcat
     5281 ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt tatataagaa
     5341 tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag ataattcatc
     5401 aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg
     5461 tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact cttgagggaa
     5521 ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa cataatagct
     5581 gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt cttcgattct
     5641 tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt ctgccagtcc
     5701 aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt
     5761 tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata ctttttcgat
     5821 ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat atctttttcc
     5881 gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct gtcttccatt
     5941 tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg cttctctaag
     6001 acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa aaacagccac
     6061 cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt ttgagatgca
     6121 agatactcaa ccaggcaatg ggcacataat taaagaaaaa actgattctt tggcaaaaac
     6181 ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt ccataaatcg
     6241 ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg tccatttgcc
     6301 cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta
     6361 aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca tgggtattga
     6421 tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg gctgctgtaa
     6481 ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca ggcaatgaca
     6541 ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt acaggactgg
     6601 aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga taatacaatc
     6661 ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag aaaaaaccaa
     6721 atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt ttaattgtta
     6781 ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca tctatataac
     6841 tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac gttcacttaa
     6901 tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg cgcctagctc
     6961 tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca ttgaatcctg
     7021 gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat tagagatacc
     7081 aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc
     7141 acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag gtgcttcaat
     7201 ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat
     7261 attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca atttcaatct
     7321 ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg
     7381 aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt taataaacaa
     7441 aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga tcaaaacaag
     7501 taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg attgataaac
     7561 caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt gtgacaacgc
     7621 tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa aagctagtcc
     7681 caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag attgaacagg
     7741 attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt gcggtgcttt
     7801 ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta caataactgc
     7861 tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct tcacgaatgt
     7921 cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg
     7981 tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa atgtccatgt
     8041 ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac gttgaactaa
     8101 cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta acaatcattg
     8161 tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac aatacaaaca
     8221 accatttctt gctaattttg tactttttct gtctacacgt tgttttttga gatattctaa
     8281 gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt ttgtccaatt
     8341 tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa
     8401 tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca gtcactattc
     8461 ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa acttccgttg
     8521 ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa atcgcccgtt
     8581 taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc acactagttt
     8641 gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc
     8701 taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta
     8761 gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac
     8821 tattattttt aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg
     8881 cattacagaa tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt
     8941 tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct
     9001 tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa
     9061 atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt
     9121 tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa
     9181 attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt
     9241 atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa
     9301 atatcgggca gttttttatt tgatatttat tagcagtgtc cctcctagtt atctttttat
     9361 tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga
     9421 ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt
     9481 attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac
     9541 caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag
     9601 cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt
     9661 ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt
     9721 cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc
     9781 tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat
     9841 tgatccttct cctattattg attactagta ttattgtaca acgtagacta caaaaacagt
     9901 attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt
     9961 tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat
    10021 ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca
    10081 ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag
    10141 aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa
    10201 gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc
    10261 ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta
    10321 taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca
    10381 tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac
    10441 cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg
    10501 aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata
    10561 aatttggttt tgacaggttc tattatttag acagtccgga taaattaact tatacggata
    10621 aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt
    10681 tacaaagcaa tacggaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc
    10741 cttatgatga ttactatagt gaattgaact acactgctga aggaactgct gtcattccta
    10801 atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa
    10861 aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg
    10921 accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc
    10981 agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata
    11041 aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag
    11101 taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg
    11161 atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata
    11221 gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc
    11281 gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa
    11341 aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc
    11401 cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt
    11461 cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg
    11521 tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat
    11581 atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc
    11641 cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt
    11701 tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac
    11761 ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg
    11821 atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg
    11881 aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact
    11941 ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc
    12001 tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg
    12061 attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc
    12121 tttttttcaa ttaaaaaact cctttcgatt actcccactt ctttattcta tcaaataagc
    12181 aatgtgattt tattgatttt tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg
    12241 agaagcaccc gctgatgttg tctgtaatac atcagcgggt gcttctttta ttttaatctt
    12301 cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat agacttttag
    12361 tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag tctttgtact
    12421 tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg caagccatat
    12481 ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg gaaatcaaaa
    12541 tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg accatgttcg
    12601 taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc attttcaatc
    12661 actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata ttctgaaaaa
    12721 agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg gatcaatttc
    12781 atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc agttggttta
    12841 tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac tatattcatt
    12901 gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt ccacaactgc
    12961 agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt tgctcgcatt
    13021 aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt cgattttttg
    13081 catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca tcgtagttct
    13141 tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa aattgattct
    13201 tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata aacgcatcgc
    13261 gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag ccatgtaaat
    13321 aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa tcactacatt
    13381 ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca acgattcaaa
    13441 attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc ctgatcccat
    13501 agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat ctccaataaa
    13561 agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt cgtctggtaa
    13621 taattcagca tggacttcat caatgcctaa catatcagct acataatcag cagtcatctg
    13681 attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact tagctaattg
    13741 ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga tttttttgtc
    13801 gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg
    13861 ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt
    13921 atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat ctttgactgc
    13981 taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc gccccagagg
    14041 atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt catcctctgt
    14101 atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag ttttatcaaa
    14161 gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga ctaagatgcc
    14221 ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc
    14281 gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac taatcagacc
    14341 aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc gatcaatgaa
    14401 tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa ttttggcaaa
    14461 ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt
    14521 tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac cagtcactgc
    14581 ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa tagctccacc
    14641 agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt cttcagtcgt
    14701 tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag ccttgattga
    14761 ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa acaagaatgt
    14821 aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag caatagtaac
    14881 gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga tcataatagg
    14941 tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga caaaaccagt
    15001 aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga tatgatttcg
    15061 atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt cattcttacg
    15121 agactaagta tagatactta tccatcataa aaaattgacc actatcaagt ttagtcaaaa
    15181 aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag actcactaaa
    15241 agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat tttcctgtac
    15301 agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat tttttccttt
    15361 agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc tgtaaaaagg
    15421 aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt accgatgcct
    15481 tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc
    15541 tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt actttcttta
    15601 gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc aaaagaacga
    15661 tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc gtcctctgca
    15721 tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata tcttccgtaa
    15781 agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc aacagcaaaa
    15841 tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt catcatttcg
    15901 cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt gtctactatc
    15961 tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga tatgtcggat
    16021 aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa aaagctttgg
    16081 ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact atacgcgaac
    16141 taacagaaac ggcagatgta catcgttcga ctttttatac acattataag gatatctatg
    16201 atctatatga t
//
LOCUS       DAZKPO010000066        15762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000066 DAZKPO010000000
VERSION     DAZKPO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.067"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1554
                     /locus_tag="ACG8NZ_002103"
     CDS             127..1554
                     /locus_tag="ACG8NZ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0539761.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1646..2011)
                     /locus_tag="ACG8NZ_002104"
     CDS             complement(1646..2011)
                     /locus_tag="ACG8NZ_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0539762.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2008..2373)
                     /locus_tag="ACG8NZ_002105"
     CDS             complement(2008..2373)
                     /locus_tag="ACG8NZ_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0539763.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2565..2813)
                     /locus_tag="ACG8NZ_002106"
     CDS             complement(2565..2813)
                     /locus_tag="ACG8NZ_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539764.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2812..2940
                     /locus_tag="ACG8NZ_002107"
     CDS             2812..2940
                     /locus_tag="ACG8NZ_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0539765.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2997..4691
                     /locus_tag="ACG8NZ_002108"
     CDS             2997..4691
                     /locus_tag="ACG8NZ_002108"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0539766.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4758..4964
                     /locus_tag="ACG8NZ_002109"
     CDS             4758..4964
                     /locus_tag="ACG8NZ_002109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539767.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5342..6475
                     /locus_tag="ACG8NZ_002110"
     CDS             5342..6475
                     /locus_tag="ACG8NZ_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0539768.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6506..6826
                     /locus_tag="ACG8NZ_002111"
     CDS             6506..6826
                     /locus_tag="ACG8NZ_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0539769.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6855..7319)
                     /locus_tag="ACG8NZ_002112"
     CDS             complement(6855..7319)
                     /locus_tag="ACG8NZ_002112"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539770.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7451..8704)
                     /locus_tag="ACG8NZ_002113"
     CDS             complement(7451..8704)
                     /locus_tag="ACG8NZ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0539771.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8733..9050)
                     /locus_tag="ACG8NZ_002114"
     CDS             complement(8733..9050)
                     /locus_tag="ACG8NZ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0539772.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9076..9396)
                     /locus_tag="ACG8NZ_002115"
     CDS             complement(9076..9396)
                     /locus_tag="ACG8NZ_002115"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0539773.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9860..11275)
                     /locus_tag="ACG8NZ_002116"
     CDS             complement(9860..11275)
                     /locus_tag="ACG8NZ_002116"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0539774.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11292..12608)
                     /locus_tag="ACG8NZ_002117"
     CDS             complement(11292..12608)
                     /locus_tag="ACG8NZ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0539775.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12758..13456)
                     /locus_tag="ACG8NZ_002118"
     CDS             complement(12758..13456)
                     /locus_tag="ACG8NZ_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0539776.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13745..14734)
                     /locus_tag="ACG8NZ_002119"
     CDS             complement(13745..14734)
                     /locus_tag="ACG8NZ_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0539777.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14814..15647)
                     /gene="msrA"
                     /locus_tag="ACG8NZ_002120"
     CDS             complement(14814..15647)
                     /gene="msrA"
                     /locus_tag="ACG8NZ_002120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0539778.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4908 a         3192 c         2695 g         4967 t
ORIGIN      
        1 taacataaaa aacctttttt aacggcattt atatgctttt tcagaaatta actgatgatt
       61 tcaaaaagaa aacatgtcat aatatttccc atattgtgca aaaaaataca aggcggaggc
      121 tctactatga ttgaagaata tattgagaaa aatattttaa gacagctgtt tttatgtggg
      181 caattctatg taaataaaga agtcaatcta gaaaagctct ccaatctcct gcatgtttgc
      241 aaaaccactt tactcaatga tatcaataac ataaaaaaag aatttgaaga acagatcgct
      301 tatacacatc gggaaaagga ccgttacact ttatattttt cagaacatat acctcgatgc
      361 aagatcatgc agcaattgtc tcaaaattcg ttgtttttaa aaacttgtct gctttatttg
      421 gaagaagatg aacctgatta tcttcaatta actgaatgtg aattcatctc tgtcagcaaa
      481 gcttattcat taaaaaaaca agtattagct tattttaatg actgcgggat cgaaatcgac
      541 cgctactccc ctcgattcac tgaaatggaa agacgtctgc tgcttcttaa tgtgtcgtat
      601 cgtttaggcg gttttaattc ttgggagcta cctgaatcgt tttttgaacg tgccgatcgt
      661 ttcattgaat ccgtaacgga aaatagtggc agattttatg ataaagaaaa taaagaaatc
      721 ttatcgattg gttttgctat cagtttccta cgccagcaag tgtgtgctgt gacaatagat
      781 tcaaaattta tcgaagaaat caaaaaacgt cctatttaca attatgtcga atcagcatgg
      841 gaaaacactg atttccaaac ctattataaa aaagaagaat tcgcctttat tttgacacta
      901 ttcaatcttt gtaattatgg tttccattct tatcagttga ttgcggaaga ctttcaacag
      961 ctacaccaag tgtttattga caatactccc gaaataaaag aactagttgc tacctttgaa
     1021 tctcacttca atcaagaatt attcggaaac caaccgttcg aacgtgcgct gattcatctg
     1081 atgcgttcgg cttgggataa ctatcaatta ttcatgccag aaaaatttta tcttttaaat
     1141 gaggaacaga caaatttata taaagaagtc cagacaattt tttccagctg gtccagccag
     1201 ttaccttatg atctacggtt gaatcctaat tgcatgcggg cttttgtgat cgaattgagc
     1261 ggaattctcc gtctgacaaa agaacatctg actatctata tcgtcacaaa ttcggatgtt
     1321 cattatctga tttatcggga agccttggaa gctgtaacta ctttcgattt tcaagttgca
     1381 ccaacaattt attcaagtat atctgatata aagaagtatg cgcagcagtc ctcaaaccgg
     1441 gtattatgtg aacgtacatt atatacacca gatgcggtcc aatatgaaaa tattattcct
     1501 atttcgatca atacgatcga tcgagctatc atttcagctg tgcaaaacaa ataaggggct
     1561 gtgacaaaaa tcatgtcaca gcctcttatt tgttgaagat gcccatttct gtaacaatct
     1621 cttgatacac gtcaggctca caacctcact tattttggac atctacgggt ccgatctctt
     1681 tatttaaaaa ataagaaatg atggttcgga taactaccaa cgccgctagt tggaaaatat
     1741 cctgcaaggt aggtttgata atcgattcga tgatcccagc tgaaacaaga atttccaaac
     1801 ttagaagtat atagcctccc aacatctttt taatagcatt gttctcgctg actcggtcgc
     1861 tttgcacttt tgtatggaca aataaatatt tcaaaaaatc cttgattgat agacaaacac
     1921 cccaaaccaa taaacagata gcaaagatat caagtcccag tacaagtttt tcgaaatatg
     1981 gaagaagaaa ctgcatcaac tctgtcgtca tcctacctct cctcttcttt gtttttcttt
     2041 gtcttctatt tcctgattca agaaataaga aatgactgtt cgaatgataa taataagtgc
     2101 aagcttcaaa atatctgtcc aagtcggctt gataactgac tcgatgatat cagcaacgac
     2161 tagtacttcc aagcttaaca aaatataact tgctagcagt tttcttgtat ctgcattacg
     2221 agtattacgt tctgcatagt ttttcttgac atgagaccaa tcaaaaagtt tgaatagaac
     2281 aagaacgatc ccgacaatta agataccgat agaacaaata tttaaaatag caatgatcca
     2341 ttctagaaat tcaattaaat ttgagcgaaa catatacgct acctactttc ttttaatttg
     2401 ttcctattct tttattgtaa aagaaagtta ccttgttcgt ctatttaaaa gttgttctaa
     2461 tgataacggt tcgcttaaat ttttcttttt cactctccca tttttctccc tccatccata
     2521 aatcgtggaa gtatccaaaa caacgtagca agttaaaata aggcttagtc cgtcatcagc
     2581 ttttcaaaga agcactgtct cggtgctttt tttgtaccca aatttctaaa aatagaacca
     2641 tacacctcct caaatctttt cttcttacct accctatttt tatttacagg aaaatcagac
     2701 acttcttctt ttttgtcaaa atttcctttt tcttctcgtc ctatcatttg gcattctaat
     2761 aaaaacgaac tatccttgga tatagaaaga gaaaacaagg aggcgttttt catgagtaaa
     2821 aaattaatca aagtcggcat cggtttaggt ttgcttgccc ttggcgctgc atatctagga
     2881 aaaaagacag gactattcga agatgacagt catttatatg atgaattcga atcaatctag
     2941 catcctattt agttgaatca ctacggacat acttggaaga cattggagga atcacaatga
     3001 gaaaaagtaa agcaataatg attggtgccg gtattgccaa tatggcagcg gctgtttatt
     3061 tgattcaaga aggcaaatgg aaaggggatc agatcacctt ttattcattg gatgatcacg
     3121 gttcaaatga tggtgcaccg accgacactg tagtggatga atattggaat aaaaaccatc
     3181 cattagaaaa ccaaaaaggc tatattgctc gaggcggtcg catgcttaat taccgtacct
     3241 atgtagattt gatggatctg ttaagtcgaa taccttctgt tactgagcca gggatgactg
     3301 ctgaagaaga tacgagagat tttgatgcaa aacaccgaac ctttgacaaa gcacgtttga
     3361 tggaaggtgg caaaggaatc attgatggcg gaaaactggg attcaataat aaagaccgta
     3421 ttcttctgac taaactgatt gctatgcctg attcagaaga agaaaaacta gacaatatca
     3481 ctatcgaaga atatttcaaa gatgatccac acttttttga aacaaacttc tggtttatgt
     3541 gggaaacaac atttgctttc agaacacgta gttctgctca agaattgcgt cgctatatgc
     3601 atcaaatgat ttatgaattc actcaaatcg aacatctagt gggcgtcaat cgaacacgtt
     3661 acaatcaata cgaaagtatt atgcttcctt tgatcgagta cttgaaagaa caaggatgca
     3721 aaatcatttt gaaccgccgt gtcactgact gggaattcaa agaaacaccg atgcaagatg
     3781 aaatcactgt gactggtttg aaaattacca atacactgac tcaagaagaa gaatacgttg
     3841 tggtcgatga agatacagca gttattttca ctaatgggtc cattacagat tcagctactt
     3901 taggagatta cgaaacacca gctgtagaaa atatggatta tggtgcagct tctagtctct
     3961 ggaaaaaagc cagtgaacgt ttctacaact tgggaaatcc tgacaaattc tttgctgatc
     4021 gagatgcaag cgaatgggtc agctttaccc tgacgacgaa aaaccatttg ttgttgaatg
     4081 aaatcacccg tatcacgact caagaaccag gaaatgcctt gaattcattt atttcaacca
     4141 caccgatcac gccgttaggc caaaaagatg tcaatatgtc tattgtcgtg catcatcaac
     4201 cgcattttac gtctcagaaa ccaaatgaaa cagttatttg gggttatttc ctttatccaa
     4261 gacgacgtgg cgaatttgtg gataaacagt atattaaaat gaatggaaaa gaaatgctag
     4321 aagaactgat tggacaactt tcaaaagtag atcctggacc agtgaatatt cgtgaaaaag
     4381 aaacagaaat tttcgacagc gtaataaata atatccctgt ctatatgcct tatgcttccg
     4441 cgttgttcaa taatcgtgcc aaaagcgatc gaccaaaagt cattcctaaa cattcgacca
     4501 atctggcctt tacaggagaa tttgtcgagc agccttatca gatgatcttt actgaacaaa
     4561 gtgctgtacg ttctggagaa atcgctgctt tccattttgc gggtattcct atgtcccgtc
     4621 ttgtcaaaac accgcgttac gacaaagata ttcctactct agctcgtgcc gcaaaaaaaa
     4681 tgtttgaata atcaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca
     4741 ggctaaaaca gcttcttatg tttttgattt ggttggactt attaccgcag atgcaagctt
     4801 ttgaacaccg tttaccaaga ggttatgaaa aagctgccct gcatcaagta ataagagcag
     4861 ccaagctaaa acagcttctt atgtttttga cttggttaga cttattaccg cagatgcaag
     4921 cttttgaaca ccgtttacca agaggttatg aaaaaggcgt ttagtttcga gtatgaagaa
     4981 aaattttgtg aaaatggttc ctcacgtttt tgcataaatt tgacttaata ccgagaaact
     5041 gatcccttct gtcataacct ctttttattt atgtctgttt ctttttgaaa acaactattt
     5101 tttcaacgtt tactctcttg ccaatccaac atcatctaac ggatacaact atttcaatga
     5161 ttaccattac actaaaaaat cggaaacaca cctctccgaa cacaaataca taagactata
     5221 gaatgtttca tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc tcctttaaaa
     5281 tgaaattatc tgtagtacta ataatgcaga caattttatg aatctgaaag gagcatctcc
     5341 aatgaaagca gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg tagatgatcc
     5401 aaaaatatta gaaccaacag atgcaatcat ccgtatcaca gcaacagcca tttgcggctc
     5461 cgatctgcat ctttaccatc atggtgaagc agttttggaa aaagggtatg ttgtaggaca
     5521 cgaaccaatg ggaatcgttg aagaagtggg atctcaagtt caaacattga aaaaagggca
     5581 acgtgtggtt atccctttta atattagttg tggtcattgt catttctgcc tgaatcacat
     5641 ggaaagccaa tgtgatgaat caaatccaga tcagttatat ggcgggcttt tcggatttgg
     5701 caagttgaac ggtaatcatt ggggtggtca agcggaatat ctaagagttc cttatgcaga
     5761 ctctacttca tttgttgtac cagataatga tcttcctgat gaaaaagttt tatttctatc
     5821 agatattttg cccacagctt attggagcgt tgaaaacgca ggagtaaaaa aaggcgatac
     5881 agttgtgatt ctaggttctg gtccagtcgg tctattcgct caaaaattcg ctgttatggc
     5941 tggagcagag cgcgtaatcg cagtagatcc agtacagcac cgtctagata aagcggctaa
     6001 ttacaatggg gttgaaactt atttcttaga agacacggca caagctggaa cagaattgta
     6061 tgaactggct aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg acggattaga
     6121 gcctgtcaaa gaaaaagcga aaaatttagt aagcttacaa agcggcacaa tctctccatt
     6181 acagatggca tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg gtgtttatat
     6241 gactccagct tcttcttacc cgctgcaaga attttttatg cgggatatcg ctgtcaagca
     6301 tggacaggca ccaattatcc atctaatgcc aaaaatttac gcaatgattg cagaaggact
     6361 attcgatcct acacaaatca ttactcattc gctgccacta tcagaagcag cacaagcata
     6421 tgagatcttt gacaaaaaag aagataaaaa tataaaagtt gttttgaaac cttgaattca
     6481 gtaaaaaata caggtgattt tctttatgcc caaaatcaaa cgaaataccc atgagatcga
     6541 aataggaaaa gaagaagata tcgaaatacg aggacgtcgc ttccgcttct atttccaaaa
     6601 tcgttacaca ttgatctcac tggctgtcga tttattaacc gggattttct atatttttgg
     6661 aagccttgct tccttaacta aaatccccga tcgatatggc acctattttt atttggctgg
     6721 tgctattttc ttgacgattc gtcctattct cagaatcgta aggaacattt ttatttacga
     6781 cgaaaaaaaa gcaatgaaga gaacaagcaa aaaaaaagag gcatgagaat ttttcaaatc
     6841 tcatgcctct tttttcaccc tatcgtatca ctttgataca gacgagcggt cgtcccactt
     6901 tcttccttga ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt ggtatgatct
     6961 gtaattaagt aaagtttttc gtacccaagt tgatgcattt cttttctgat ttccgctaat
     7021 atccgcttgg ctatcccctg cttacgcttt tccggttcaa caaacagggc acaaagattt
     7081 ggtgtcagat cagggcggtt atggaagtcg ttttcaatga cgcccgctcc tccgacgatg
     7141 gtttcttttt catctaagac cacataccat tgaggaatac tttccttatt agcgattcca
     7201 gtacgaatac tttcctcata cacaacagca ggaactcccc attttgatga aaaccaattc
     7261 gcgctttgtt ttactaattc tgggtgttct cttaatttga tccagttata tgtaaccaat
     7321 gtttaaacct ccgactaaaa taaaacgttt acaaaaatta tacaagtctt tatgaaaatt
     7381 agcaatgaca acaatagtga tgtaaaaaaa aaaacggaaa acaatcactt gttctccgtt
     7441 ttttgattaa tcaagcatct aaagcttctt ttcttctttc ttggatcgta gctaccttga
     7501 cgaacggtag atagatcaaa atagaaagaa ccaaattgac agcggctagt acaccacctg
     7561 caatactttt tgttgccaat acaccgccaa taattggtgg agttacccat ggaggcataa
     7621 cggtagctgc tggcactaaa cctgtagaag tcgcaaagta agcaacacta actaagacca
     7681 ttggtgtcaa aataaatgga atgaacatga tcgggttcaa cacgataggt aacccaaaca
     7741 tcgttggttc attaatattg aagacacctg gcgcaatcga caagtttgtt acaaccatat
     7801 atggtttgtt tttacgtcct actaaataaa tagctaacag taaacctaaa gttgcacctg
     7861 ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac ggtgcgctat
     7921 gtccagctgc gattgcatta acgtttgctt caatggctgg cgcattgatt gtttgcatca
     7981 aaggatcgac catattcgct ccgtgtaaac caaagaacca taagaatggt gtgataaatg
     8041 caagcagtaa ggcagatgga taagagtttg caagtcccat aaatggttct tggaccactg
     8101 tatagaaaga acctacgata tcatcaacac cgaatgcagc aaaaattgca gcaactaaag
     8161 caaacgcaga gatagtgatc attgctggta gtaatgaagc aaatgctttt gcaactgccg
     8221 gtggcacacc atcaggcatt ttgatgatca atttttcatt tccgcttaaa cgtacaaaga
     8281 tttctgttga aataatccca acaataatag caataaacaa accgtttgag ctcataccta
     8341 atgctccgcc taaagcgaag aaagagccta aagaaatcac acctgcggaa acaccgtctt
     8401 ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc aatcaagaca gaaagaatgg
     8461 caaatgtacc gttccaagca gcagccccga atcctttcca tgattcacca ccaaaaattg
     8521 agttcatcaa ttcttggaac ccgggaatcg gtaagttatt gatcattact gctaatgctc
     8581 ctaagatcat taacggcata ctcaccataa atgagtcccg aatcgcaact aaatgccgtt
     8641 gtgcaccaat ttttgatgct acaggtataa aatgtttctc cataaatgca acaaatttat
     8701 ccataaatct tccctctttt ctttactgta gattacgcgt tcaatcgacg atacagatca
     8761 atgatctctt ttgctaaatc tgtaaaagct atagatgtca tcagatgatc ttgactgtgg
     8821 accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag catctctgtt
     8881 tgagagtgat gtgcttggac taaagattcc tctgcatctt tgattttttc atctgctaaa
     8941 gtgaaatcac cttttttcgc agcagcaatc gcttccattg catcgctttt tgcatttccg
     9001 ccaaacatga tcagacccat gatggcttct agattttttt gatcttccat ttattgtatc
     9061 ctccgataat ttttattatt tgtttattag tttttctgct tggtcgagta ctttttcacc
     9121 attcatcatg ccatagtcag ccatattgat tacatctaaa ggaatgcctt taggtgctag
     9181 cttttcagca aattgatttt gcataaagcg tacttgcggt cctaaaagta aaacgtctac
     9241 atttttagat tctagttgat tatccgcatc tgaagcagat acagcaaaga tgtctgcttc
     9301 cattcctttt gcttctgctg ccttttgcat ttttgtgact aggagacttg tactcattcc
     9361 agctgaacat acaagcatga ttgttttctt ttccatttta aacacgttcc tttctatact
     9421 tacacttgta tataagcaag tagcgtgcca actttaaaag cgctttaaaa agttgatatc
     9481 atcacattta aggtctatac caagttgcat acacactaaa aaagaaagcg cacactaaac
     9541 acacaaatta atttttatta ccttttttct tttaacgtgc acaaaaaaag ttgtgacaca
     9601 aaacttgtca caactttttt caaataaacg gtattcaaaa gcttgcatct gcggtaataa
     9661 gtccaaccaa gttaaaaaca taagatgttg tttttacttt gctattctta ttacttgatg
     9721 caggacagct ttttcacaac ctctcaataa acggtgttca atcagctgac cctgggtatt
     9781 agctcaaaac gctggattca caacctcttt tatttcaaat aaccagtaaa tacaatgaac
     9841 atctacaaaa aacttctatt tatttataga tttcgtcagg caaatccaat tctctcttat
     9901 caatcaaagt ctgataccac gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca
     9961 attcgattcg aactaatcca tatcgattct taaatgcatt cattggtgac acacagtcag
    10021 tgaatgccca taacatatag ccttcgcaat ttgcgccttc ttctgttgct ttgatcaagt
    10081 aatgcaaatg ctgtccgata aattcgatac ggtaatcatc ttgcacgatt cctttctcat
    10141 ccatatactg ttcttcatat tctcttccca taccgttttc tgttatcagc caagggatat
    10201 tatgataatt ttcttttaga tacatggcta tatcatacat gattttcgga tagatttccc
    10261 atcccctgga attattcatt ttcttcccag gtaaatcaaa gacttcataa tatcgttcag
    10321 gatggaaagg tgtctcttca ttccattgat aacgaggggc gcgaacgcgt ttaggataat
    10381 attgattgat tccaaggaaa tctactgtat gatgctgaat cgtttctaaa tcctctgttg
    10441 caggatcgaa ataaatcgat tgtttttgac acaactccag gaactcttct gtgtagtatc
    10501 cttttaccat cggatcaaaa aatactcgat tataaaacaa atcatacata taagcagctt
    10561 tttggtcttc tggagaattg gatcgagcat agaccatttc aggatttaaa atacagccaa
    10621 tccttccacc ataatttccg ctgcggtaaa tctctactgc tttagccgtt gctaaaactt
    10681 tatgataatt ccacatcatc cacttttttg tgttttgttc atgcggccag cgaacagcat
    10741 ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc
    10801 tatcaccgta acgatcaaag gcaatttgag catacttagc ataaaaatca actacttttc
    10861 ttgaagacca gccatcatat ttttccaaaa gaaaagctgg gacttcataa tgttccaaac
    10921 aaatcatcgg ttctacgtct gcttcgatca aagagtcgat catttcactt atatgctgag
    10981 catattcttc atctactata cagttctcat aatcagtaaa aaaacgagcc caattgatag
    11041 aagtccgata atgtgtcaga tggatggatt tcatcagatc cacatcctgc tggtattgtt
    11101 ccataaaatt agttgctacg gctgggccat acccattatg ccaaacaaaa tgttcatttt
    11161 tataccagct atctataaac gaatcctgtc cttctttttt tcctttccaa ccttccgttt
    11221 gccaagcaga agatgcagca ccaagaataa aattttttgg aatagtaatt ttcatgattt
    11281 cctcctgttt tttagatccc atcattttcc acacgatttg ctacttttac aaatggcaga
    11341 tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa taactgcacc gatatttcct
    11401 gctgttgcca gataagcatt gacgatcggc ggcattggcc aagggaccat tacaactgct
    11461 ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg ttccagtgat aagaggcgtt
    11521 aagataaacg gaatagctaa aattggattc agcatgatcg gcataccaaa aataactggt
    11581 tcattgatgt tgaagatccc aggagcaagg gataacttgg tgatttgttt catatcttcc
    11641 cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg tgactccaga cccgcccata
    11701 ctcatataca tatcccaaaa tggcatagta ataatgttcg gtacttcttt tccagcttca
    11761 aaagcttccg tatttacagc aattgccgct aaaagcaacg gttcccgtat cggcttcacc
    11821 atttgatttc catggatacc aatcacccaa aagacttgcg caataaacat caatagcaaa
    11881 atacccggta acccttgtac gacaccttct aatgggcgtt gtacgacatt gtaaatgata
    11941 tcataaaggt acattcctgt aagttctttg atgataaatc ccgcagccgc gatacccgat
    12001 acagtcagaa tcgtcgggaa taaagcagaa aaactagcag aaacattcgt tggcacggta
    12061 tcaggcattc tcagctgtag tttcttctgt ttcctcaacc agcaataaag ttcaatagaa
    12121 aggatggaga ccaacattcc caaaaataac cctttcgtat ctgtatattg tttagccaat
    12181 acgttttcta ctataacagt ctgattttca ttcactaatt cataaacagt tggattaaca
    12241 gataaataag acatcactgc gatgatacca gggaatacac catccacttt attcagcttg
    12301 gctaattcga tccctatcag aaaaacagca tagatggtca agaaacttag tgtgacatag
    12361 ctgatggcag atgcgatagg ctttaattct tctaaaaaac gcaagaaact tatctgtgct
    12421 aagccatttt ccgagtcaaa gaccattgct gaaaacaatg tgccaaaagc acccgtaata
    12481 attaccggaa tcaatgcagc aaatgcgttt tttatcacca tgatatacct taaattattg
    12541 attctcgttg ccacgttccc taaaacattg gctaatttat ccataaaact cattccagat
    12601 cccctcattc ctgcttcttc tccttgacgt catctctttt tgtctattag tcagcgctta
    12661 catttcgatg aacagaaact atttactcct cttttttacc aaatataaaa caaaaattca
    12721 tctgatgtat ttatcataaa atacatcaga tgaactgtca attagtttct ttttctccta
    12781 ttattttctg taattctctc cgatttattg ccatatggac agtcattgca tcaaacgcag
    12841 caagtgcatt ccgcctttcg atggcctgaa cgatctctgt atgaatatcg atcgtctcga
    12901 ttttcagatg ctggatatca taatttttat taaacaaagc aattgattca ttaatgattg
    12961 ggataatatg cagcaaggct acgtttccgc tagcttctgc aatcatcgta tgaaaatcga
    13021 tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga agtttcaatc ttttgcgtta
    13081 attttttcat ttggacgatt tgattcttcg ttgcatattc cgctgccaaa cctgccatct
    13141 taggctccaa taaatatctc agttcaaata agtctcgtat catccgatcc tgatcttcaa
    13201 tcaaagaaaa accgagtggg tcattggcaa tccctcgctt atcactgaca aatgtccccg
    13261 ctccttgccg aacttcaagg atattcctgg aagctagagc ccgcacagct tctcgcaccg
    13321 tgcttcgccc cacctctaaa taatcggaca attcatattc attcggcagt ttatctccta
    13381 cttgcaactg catttccttt aaatatctaa ggatcttgtc agtagttact tctactaggg
    13441 ttttattttc ttccaaacca catacctcct tttcatctga tgtatttttt cgtaattatt
    13501 ataacacgtt tgaatcatct gatgattttt tgtatacaaa attgattaat ccgttcatag
    13561 acatacaaac gaagaagata tttaccataa aaaagactag gataaaattc actcctagcc
    13621 ttcgatcatt tttattttga acgacaaaaa atatgatgtt caataacaga cgatcttcat
    13681 tcgattcttg atgtcatctt ttgttttttt taatatatgg ataaaaagtt tgattacatc
    13741 gtttctattc tagaatttgg tcgattctca attcttcttc tggtgagaaa tctaaattat
    13801 ccatcattcg aacactttct tgtaactgac ttaaccgact tgctccgacc agtacacttg
    13861 taacagattt ttgtcgcaaa ttccatgcaa gggccatttg cgctaatgat tgtcctcgtg
    13921 atacggcgat tgtttgcagt gctttgattt tttccagagt tgatccaact tgttcttctg
    13981 aaaggaatgg tatttcctta cgatgtgctc tagaatcttc aggaatacca tgtagataac
    14041 gatttgtcag caacccttga gcaagcggac tgaacgtgat tgcccctagc ccttcttctt
    14101 ctaatactgg agatagatcg tcttctagta gtgcacgaga aaacatattg tagcgcatct
    14161 gatgaataat gaacggtgct tcctttcttt ttaaaatttc agtcattttt tttgtatctt
    14221 ctccattata gttggatata ccaatatata atgcttttcc ttgtcttaca agttgcatca
    14281 aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga tagaagatgt
    14341 caacataatc tagccccatt ctttggagac tttggtcaca acttgaaatg atggatttac
    14401 gagagcccca ttccccatat ggcccttccc acatatgata acctgctttg ctagaaataa
    14461 tcagttcatc acggtaggct tgaaaatctt ctctaaaaat acgcccaaag tttttctctg
    14521 cagctccagc agggcctcca taattattcg caaggtcgaa atgggtaatt cccatgtcga
    14581 atgcgcctct taaaatctct ctttgattat ggataggatc ataatcacca aaattttgcc
    14641 ataatccgag agataaaatc ggtaatttca aacctgaatg accggattta cgatattcca
    14701 ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat caaatctttt
    14761 taaaacgctt tcttttttct atttttagtt tactacattt taaagaaaag gagttaatca
    14821 aaatgatttt ttcgtatatt tttttgccat ttgtttttta ctcgttgata gatcagaaat
    14881 tgattacgtg cttgctggat ttttttataa cgtttcggtt gttttttgta aaattgctga
    14941 tgatagtttt ctgctggcca aaacgtaccg gctggttcga tttttgtgac gattggttgg
    15001 tggaaagttc ctgaactgtc tagtttctgt tttgattgtt ccgctaattc tttttgtcgt
    15061 tcgacttgat aaaaaatcac tggacgatat tgcttccctc tatcttgaaa ttgtccaaat
    15121 gtatctgtgg gatcagtaat ttgccaatag attgtcagca actcgctata acttatttct
    15181 cttgtgtcat aaatgatctc cacagcttcc acatgacctg tgtaaccacc acttacttga
    15241 tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacaga taggatccct
    15301 tttttcgatt caaacggctc gaccatacac cagaaacatc cccctgcaaa tactgcacgt
    15361 tcttgatagt cagcatcttg catggcatgc ttggaaaagt catatttttg ctccttatca
    15421 aatccttccg taatcaataa ataaaagtcc attacatcag gcgtcagatt cattcggata
    15481 gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttaacgga tatattttct
    15541 cttatgtcat tctttgtctg aatcaatact tttcgttccc aatctcgtgt attcggattc
    15601 aaaatcagat tataaagtgt ttgaatgaat atttgttcct catccataga ttctcctcct
    15661 ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataagc
    15721 gattacttca tccccctaca gatttttcgt ataaaaaaga ga
//
LOCUS       DAZKPO010000067        15741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000067 DAZKPO010000000
VERSION     DAZKPO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.068"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8NZ_002121"
     CDS             complement(24..722)
                     /locus_tag="ACG8NZ_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0539779.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8NZ_002122"
     CDS             complement(715..2067)
                     /locus_tag="ACG8NZ_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0539780.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8NZ_002123"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8NZ_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0539781.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8NZ_002124"
     CDS             3851..4588
                     /locus_tag="ACG8NZ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0539782.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8NZ_002125"
     CDS             4713..5192
                     /locus_tag="ACG8NZ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0539783.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8NZ_002126"
     CDS             5189..5956
                     /locus_tag="ACG8NZ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0539784.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8NZ_002127"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8NZ_002127"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0539785.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8NZ_002128"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8NZ_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0539786.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8NZ_002129"
     CDS             7567..7797
                     /locus_tag="ACG8NZ_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539787.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8NZ_002130"
     CDS             7788..8036
                     /locus_tag="ACG8NZ_002130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539788.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8NZ_002131"
     CDS             8142..8726
                     /locus_tag="ACG8NZ_002131"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0539789.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8NZ_002132"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8NZ_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0539790.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8NZ_002133"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8NZ_002133"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0539791.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8NZ_002134"
     CDS             10627..10806
                     /locus_tag="ACG8NZ_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539792.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8NZ_002135"
     CDS             10828..11853
                     /locus_tag="ACG8NZ_002135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0539793.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8NZ_002136"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8NZ_002136"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0539794.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8NZ_002137"
     CDS             13425..14372
                     /locus_tag="ACG8NZ_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0539795.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8NZ_002138"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8NZ_002138"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0539796.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8NZ_002139"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8NZ_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539797.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8NZ_002140"
     tRNA            15637..15709
                     /locus_tag="ACG8NZ_002140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4374 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt t
//
LOCUS       DAZKPO010000068        15314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000068 DAZKPO010000000
VERSION     DAZKPO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.069"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8NZ_002141"
     CDS             278..787
                     /locus_tag="ACG8NZ_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0539798.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8NZ_002142"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8NZ_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0539799.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8NZ_002143"
     CDS             2322..3302
                     /locus_tag="ACG8NZ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0539800.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8NZ_002144"
     CDS             3424..4842
                     /locus_tag="ACG8NZ_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0539801.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8NZ_002145"
     CDS             4913..6382
                     /locus_tag="ACG8NZ_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0539802.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8NZ_002146"
     CDS             6392..6859
                     /locus_tag="ACG8NZ_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0539803.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8NZ_002147"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8NZ_002147"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0539804.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACG8NZ_002148"
     CDS             7849..10383
                     /locus_tag="ACG8NZ_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0539805.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACG8NZ_002149"
     CDS             10539..11312
                     /locus_tag="ACG8NZ_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0539806.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACG8NZ_002150"
     CDS             11305..12096
                     /locus_tag="ACG8NZ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0539807.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACG8NZ_002151"
     CDS             12106..12597
                     /locus_tag="ACG8NZ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0539808.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACG8NZ_002152"
     CDS             12615..13025
                     /locus_tag="ACG8NZ_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0539809.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACG8NZ_002153"
     CDS             13029..14429
                     /locus_tag="ACG8NZ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0539810.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACG8NZ_002154"
     CDS             14452..14826
                     /locus_tag="ACG8NZ_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0539811.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DAZKPO010000069        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000069 DAZKPO010000000
VERSION     DAZKPO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.070"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8NZ_002155"
     CDS             2..1021
                     /locus_tag="ACG8NZ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0539812.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8NZ_002156"
     CDS             1018..1785
                     /locus_tag="ACG8NZ_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0539813.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8NZ_002157"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8NZ_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0539814.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8NZ_002158"
     CDS             3750..4070
                     /locus_tag="ACG8NZ_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0539815.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8NZ_002159"
     CDS             4070..4420
                     /locus_tag="ACG8NZ_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0539816.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8NZ_002160"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8NZ_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0539817.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8NZ_002161"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8NZ_002161"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0539818.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8NZ_002162"
     CDS             6414..7439
                     /locus_tag="ACG8NZ_002162"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0539819.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8NZ_002163"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8NZ_002163"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0539820.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8NZ_002164"
     CDS             8517..8957
                     /locus_tag="ACG8NZ_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0539821.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8NZ_002165"
     CDS             8960..9562
                     /locus_tag="ACG8NZ_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0539822.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8NZ_002166"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8NZ_002166"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0539823.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8NZ_002167"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8NZ_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0539824.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8NZ_002168"
     CDS             12294..12689
                     /locus_tag="ACG8NZ_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539825.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8NZ_002169"
     CDS             12689..12940
                     /locus_tag="ACG8NZ_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539826.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8NZ_002170"
     CDS             12983..13378
                     /locus_tag="ACG8NZ_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539827.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8NZ_002171"
     CDS             13375..14631
                     /locus_tag="ACG8NZ_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0539828.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8NZ_002172"
     CDS             14643..14966
                     /locus_tag="ACG8NZ_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539829.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8NZ_002173"
     CDS             14959..15225
                     /locus_tag="ACG8NZ_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539830.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKPO010000070        15072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000070 DAZKPO010000000
VERSION     DAZKPO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.072"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8NZ_002174"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8NZ_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8NZ_002175"
     CDS             706..1734
                     /locus_tag="ACG8NZ_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0539831.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8NZ_002176"
     CDS             1731..2489
                     /locus_tag="ACG8NZ_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0539832.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8NZ_002177"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8NZ_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0539833.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8NZ_002178"
     CDS             3376..4629
                     /locus_tag="ACG8NZ_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0539834.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8NZ_002179"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8NZ_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8NZ_002180"
     CDS             5300..5626
                     /locus_tag="ACG8NZ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0539835.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8NZ_002181"
     CDS             5694..6407
                     /locus_tag="ACG8NZ_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0539836.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8NZ_002182"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8NZ_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8NZ_002183"
     CDS             7690..9555
                     /locus_tag="ACG8NZ_002183"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0539837.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8NZ_002184"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8NZ_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8NZ_002185"
     CDS             11120..11821
                     /locus_tag="ACG8NZ_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0539838.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8NZ_002186"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8NZ_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG8NZ_002187"
     CDS             complement(12857..13066)
                     /locus_tag="ACG8NZ_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0539839.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG8NZ_002188"
     CDS             13307..13882
                     /locus_tag="ACG8NZ_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0539840.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG8NZ_002189"
     CDS             13882..14322
                     /locus_tag="ACG8NZ_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0539841.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG8NZ_002190"
     CDS             14338..15039
                     /locus_tag="ACG8NZ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0539842.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DAZKPO010000071        14838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000071 DAZKPO010000000
VERSION     DAZKPO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.073"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1030
                     /locus_tag="ACG8NZ_002191"
     CDS             416..1030
                     /locus_tag="ACG8NZ_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0539843.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1803..3542
                     /gene="yfcC"
                     /locus_tag="ACG8NZ_002192"
     CDS             1803..3542
                     /gene="yfcC"
                     /locus_tag="ACG8NZ_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0539844.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3699..4883
                     /gene="iadA"
                     /locus_tag="ACG8NZ_002193"
     CDS             3699..4883
                     /gene="iadA"
                     /locus_tag="ACG8NZ_002193"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0539845.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4994..5197
                     /locus_tag="ACG8NZ_002194"
     CDS             4994..5197
                     /locus_tag="ACG8NZ_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0539846.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5298..5960)
                     /locus_tag="ACG8NZ_002195"
     CDS             complement(5298..5960)
                     /locus_tag="ACG8NZ_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539847.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5973..7475)
                     /locus_tag="ACG8NZ_002196"
     CDS             complement(5973..7475)
                     /locus_tag="ACG8NZ_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539848.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8023..9702
                     /locus_tag="ACG8NZ_002197"
     CDS             8023..9702
                     /locus_tag="ACG8NZ_002197"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0539849.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9829..10308
                     /locus_tag="ACG8NZ_002198"
     CDS             9829..10308
                     /locus_tag="ACG8NZ_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0539850.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10527..11207
                     /locus_tag="ACG8NZ_002199"
     CDS             10527..11207
                     /locus_tag="ACG8NZ_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0539851.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11215..12546
                     /locus_tag="ACG8NZ_002200"
     CDS             11215..12546
                     /locus_tag="ACG8NZ_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0539852.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12561..13523
                     /locus_tag="ACG8NZ_002201"
     CDS             12561..13523
                     /locus_tag="ACG8NZ_002201"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0539853.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13634..13972
                     /locus_tag="ACG8NZ_002202"
     CDS             13634..13972
                     /locus_tag="ACG8NZ_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0539854.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14051..14758
                     /locus_tag="ACG8NZ_002203"
     CDS             14051..14758
                     /locus_tag="ACG8NZ_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0539855.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4762 a         2578 c         3191 g         4307 t
ORIGIN      
        1 tttttgtatt ttcttgcttg cacttggata agagattact tacaataaat tcagatagaa
       61 acagctgaat aaaaaaaggc tccctgaccc ttagttggcg ctaagaatca gggaatgacc
      121 agtcacacac atacaccctg accgatcatt ttgccatagt caatgcagtg catcgaccta
      181 atttattgta cctaatctag ggtgaaaatt ctacccctat gtagatattt tccatggtct
      241 gcacgttgtt ttatttgcta tgtcaaggta ttgggcgtat tcgttcaatg ccttttttgt
      301 gtttgaaaca gcaaagacca catgtgttac tgtctaacta ctgttttttt atgtacttcc
      361 ttagattagg cagctgtttc tattcatgtc aagtatagct aggcgggaga gataaatgag
      421 tttatgttat tttgactatt atcaccaatt ggaagagaaa ttaaggatag aagagccgct
      481 gatgcttcaa gctggcactt ggatcgcgaa acaaattaat caaggtggta gattgcagat
      541 atatgctagc cgcacactaa gtggtgtagc atttgaattt tgggatcaaa ccccggatct
      601 tgtaccaagt atattgattg aaaatccagc cggaggtgtc tatgaaacgc tagaaggtgc
      661 aggacaggca attattgatc agctttctgt caaatcagaa gacatttttc ttcttctttc
      721 gaatgaagga agaaacccgg caatcatcga actagctcag tggatcaaag aaaatggtca
      781 tccattaatc attgttactg gatttgattt gtcacgaagt atcaaatcgc aacatagcag
      841 tggtttgcga ttatttgaat ttgctgattt ggttttggac aaccacgcca atttacatga
      901 tgcagtcttg actttgccaa atctagatcc agcgatctgc ggaacagcct ctcttgcaac
      961 gatctttttg ctgcagcaag ttttgtattt tacagcaaaa cagctagtcc aggacacaag
     1021 tggaaaatag ttatccacat gttgacaaag ttccttaaaa aaataatgtt ccacataaaa
     1081 atggagcgtt tgaaaaaaag aaaacgatta ttaatgagga ttaacaacga ctactttttt
     1141 tgttttagtt attttaagat ggatcatctt tgattttaag cttgttttgt ttaaagtttg
     1201 ttatttttat aagttgtttc acagttatga gaaatgagtt ataagaatat gtagtgtcag
     1261 aaaaataaaa agtagtggaa aaagagacaa gaaagaatcc atatggattc tttcttgtct
     1321 cttaatacac gttgatgaaa gagtcttccc caaaactctt tacaaagtaa ttatagtaca
     1381 tttgttatca ttattgcaat gaagtttacc tgatataaca aaattgtcca acaggatacc
     1441 tttttctaat agaaataaaa tacacaaaaa ttgaaggata gataatggat atttataagt
     1501 gtttattatt ttttttgcaa tgaaattcaa tttttcaatg attgtaaaat gaaaaaaaca
     1561 ggacgagatt ttctctaatc taaaaataaa aaaagaattt taaggaaaaa cagagaaata
     1621 aaatacttaa tataaatgga aagagaaaaa aataatcata cataggagaa aaaaaacaaa
     1681 atagaaaaaa gtgattttct aataaattat tcatgtggaa atgtcatttt tactgtataa
     1741 ttcatttgat tatccatttt ttattcatca aaattactga aaaaagaaag gaagaaataa
     1801 atttgaagaa aacaaattct tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac
     1861 cccatacgta tgtcattatt tttggtgtag tcatctttgc atggatactg acttttgtcg
     1921 ttccagcagg aaagttcagc acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa
     1981 gtactcgtac tgttttacgt caagattcct tccgttatgc ttacgagctg gataagtcgt
     2041 atgtgtttga tcaattagaa gaactgcaag atcatccagc agaacgtgaa aaactggatg
     2101 ttccggaaaa aggattggaa aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa
     2161 aactagatga aatctccttg acagatgatg tgctttacga tcagtacggc gaaaatatct
     2221 atgatacttc taaaaaattg cataaaacag ccaaaatatg gggaacagat gattttggcg
     2281 gctttggttt cttaaatttt gtttttgaag gactagtatc tggagataag tatggatctg
     2341 ctgtagggat cgctgcattg atccttgttg ttggaggtgc ttttggtatt attatgcgta
     2401 ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa aactaaggga ctggagcgtt
     2461 tagctttacc gctattattc tttgcttttt cattcggtgg cgctacattc gggatggcag
     2521 aagaggtaat tccattctca atggtgatgg ttccatttgt cattgcgtta ggatatgact
     2581 cgatcgtagc agtaaccgtg acttatgtcg catctcaagt cggaaatgcg acctcttgga
     2641 tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg aatccccgtg ttgtctggtg
     2701 cgacattccg attgattatg tgggtagttg ttactgcatt agccgctggt tatatgatga
     2761 tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt
     2821 atttccgttc tcatatcaaa aagacatctg atgaaaataa aaaattcatg cttgggcata
     2881 aattgatctt attagagatg ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa
     2941 aaggctatta cattccagaa atcgcttctc aattctttgt gatgggatta gttgctggaa
     3001 tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa tgacatcgct tcttctttcc
     3061 aatctggtgc agctgattta gctggtacag caatcgtagt aggtatggcc aaagggattc
     3121 ttcttgtctt gggaggatct gatgcatctg ttccttcagc tttgaatacg attttgcatg
     3181 ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg tgcttgggcg atgtacatct
     3241 tccaaagttt gttcaacttg gttgttacat caaattcagg acaagcagct ttgactatgc
     3301 caatcatggc tccactggca gatttagtag gtgtatccag acaagtagca gttcttgctt
     3361 atcagcttgg ttcaggtttt atggacgctt ttacacccgt atcagccagc ttgattggtg
     3421 tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag attccaaatc aagatgcaag
     3481 gattcctgtt tgttttagga actattttca tcatgattgc cattgcaata ggtctgcagt
     3541 agtactggag acctgtaaat aaaaaagtaa tccaaacagt agataaggac tccaagaacc
     3601 gtcattttac ggtaaaatca aaggagtaaa gatcctatcg tttggattat tttttaagat
     3661 cctatcatca gttatactta aagaaagagg aaaaatgtat gaaaatcatt cgacaaattg
     3721 aagtgtttgc accagattac ttaggaaaaa tggatgtact gattgccggt gggaagattt
     3781 tggctgtaga agaacagcta gaaggcggct acgaaggagt ggaagtagaa gaactttcag
     3841 gtgagggaaa agtactaacc ccgggtttca ttgactgtca tttccatatt ttaggtggag
     3901 gaggagaagg cggttatcaa aatcgaacgc cagaagtcac attgagccaa ttgactacag
     3961 ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg agaaggtaga gatatgactg
     4021 cgttgatcag caaagcgaaa ggtctggaag cagaaggaat ctccacttat gtatacgaag
     4081 gttcgtaccg attgccggta aaaacagtaa cagattctat catcaaagac tttttgacaa
     4141 ttgataaaat tatcggaatc ggagaaatcg cagtatccga tcaccgttct tctcagccaa
     4201 gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt aggaggcatg ttatccggaa
     4261 aagctgggat catcaatgtc cacttaggcg gaggaaaaag aaagctagag ctattgacac
     4321 gaatcgtaga agaaacagaa attccgatca cacaattttt gcctactcat gcaaatcgca
     4381 caccagaatt atttgaagct tgcgtggcat ttgccaaacg tggtggcacg attgacttca
     4441 ctgctagcga agatcctgat ttttgggaga agacagatgg tgaagttcgc ttcagcaaag
     4501 cactgaaacg attgatcgag gaagatgttt ctttagataa tttcacgatg acctcagatg
     4561 gacaaggaag cttgccttat tttgatgaaa acaatcattt cctagggtta ggagtaggaa
     4621 gtgccaaggc attgctagta gggatcaaag aagctgtgca aaaagaaagt atccctttgg
     4681 aaatcgcctt acgagctata accagtaatc cagcacgtat attgaaattg gataaaaaag
     4741 gaaaaatcga aattggtgct gatgcagatc tgtgtatctt ggataaagaa acgcttgata
     4801 ttgatacagt tatcgctaaa ggagaaataa tggtccaaga aaaagaagtc aaagtatggg
     4861 ggactttcga aaaaagtttt taatcaacag ctctaactat cttttttaga aaacgagcgt
     4921 tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt agatcaataa acaaactaag
     4981 aggaggaagc acaatggcag atttaaaagg acgttctaaa gatatgaaag ataaagtagt
     5041 cggtgaagga aaagaagcat acggcaaagc aacagacgac aagagcaaag aaacagaagg
     5101 gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct cgaaatttag gcgacgaagt
     5161 caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa ccacttatta ctaattgaat
     5221 caagcaaata aaaaatccga accatatctg cgagacagat atggttcgga ttttttattt
     5281 gatcattatc ctgtttttta aacttcgatt ttttgaaatt ttttattttt tagttcaaag
     5341 acttcatcac agctttttgc tacttcaatc tcatgagtca cgatgataat acagcgattt
     5401 tgttcatggg cgatttcttg gaaaagtttg acgatatctt gtgaagttgt ttcatctaga
     5461 tttccagtag gctcatccgc tacgattagt tgatgttcac agcagatagc acgaacgata
     5521 gcgactcgct gctgctgacc tcccgaaagt ttggtcacct gtttttttgc taatatttca
     5581 tcaattccta cacgagccag tcgatctaaa gcaacttgtc gataattctc ttgcttggtc
     5641 ttagtgattt ccattgctgc tatgacgttg tctagagccg ataaataagg aagcaagttg
     5701 taagcttgga aaataatcga gacatcattt ttacgataac ttcttaaccc gattttgttc
     5761 agtgaacgat cttgatagta tacctttcct tcttttggtg catctagtcc agaaataagc
     5821 gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa tagcgtacat ttttccagct
     5881 tcgaaattca gttctacatc tttataaagc gcatcttgcg gatcttgata ccaaaatccc
     5941 aattgttctg tccgtaacat caaattcctc ctttagttta ataggatttt tcttgggttt
     6001 aatcttaata tccctataga agctaaaaag acagaaaaca gactgattcc aaatgctact
     6061 ccggctaata gtcccaattg tgcaggtttg acattgattt ccaattcgct gacttgatca
     6121 gaaacagcaa atggattatt cgtggttcct tgttgaccat cgcgttgacc gccttgttct
     6181 tgtctgttac ctggcatttg tccaccgcca ggagcttgct ggttttgtgt cgtggtttct
     6241 gtctgttggt caagtagctg ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa
     6301 gcaactccta atgcaagaat catacaaata gccacttcgg taaacaattg gagaatgatt
     6361 ttacttctgg attctcctaa agatagcaag acgcctaact catgccgacg ctcccgaatg
     6421 gtaatcataa tgatcagagt aagtatgata ataccggcta cagccactag taaaacaaca
     6481 tttttagaaa aacttgccac gttgtttaaa ggttccaaca ttgactgata catggaatca
     6541 tttgattgta agctaaatgt atctgtgtcg atcagcttct cagcttcttt gagaaactca
     6601 tccattttat ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat
     6661 gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg
     6721 aagttgaatt tcatacccat actgccgctt gtttctctgg attcataaat tcctttgatc
     6781 gtcatttcat aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt atctccgact
     6841 gccaggtcat ttgtttctgc tagagtagaa tcaatgacta cattgtttgt gcctttgtcg
     6901 ctgtcatcta ttccttcacc atccgtgatt tttgctgttc catcagaaaa attactattt
     6961 tgtgcagttg ctgacacacc actgatttga aagtctgctt gagtcatttg cggattcccg
     7021 cccattcctc cagcaggttc accgcctgca tcagtagttt ctgaactgtc ttcacttgaa
     7081 tcagaactag tgatggcagt tattccatct tttgccatag ctgaggcgga actttcaaag
     7141 gaataactct ttacattatc taattcagct attttttccg cgtccgttaa ggaaactggt
     7201 gtaagagaaa aactgcctgg atctggacgt tcttcagtgg tagaaccgct tgaagaagtt
     7261 tgttcctgtt ttttaaatga agcttctcga ttggcactta aagtcactgt tgctccaact
     7321 tctttttgtg cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag gccagctagc
     7381 acaaaaataa gaatggctgt gaacacggca aacaataaca atgaacgtcc ccattttact
     7441 tttgtacttt tccatgcacg tttcataaaa ttcatatttt gcatccctcc tagtagatgc
     7501 atttgataat agacaaccta tcttaaagct agcttaaatc ttatgctgtt tgcacaccat
     7561 caacatgagg ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa
     7621 tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc
     7681 ctagatgcta cagagggatt tttacgtgca tagttgttct aacaattgct gatactagcg
     7741 atttagttta tttgttgtat aataatgggg aacagctgaa tacttatata agtattctgg
     7801 ctatagcgtt tcgcaaaaga tccattttgt taatgaagta tatccaaaat aaaatgccca
     7861 tccaactgtc agaacgcgga tgtgctggta attttcctgc tgattctcca taagttgaga
     7921 aatagtgatt ttgcatatgc ttcattggaa tggctcatat atttagataa agaagaaaat
     7981 cgacatcttt aaacttggat gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt
     8041 atcccattag gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag
     8101 gatattttta ttttagattg tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac
     8161 gtcgtaatcc ctgattttac gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt
     8221 ttaacacatg gacatgcaga tgcgatcggg gcacttcctt acttgttgtc aaaaatttct
     8281 gtaccagtat tcgggacaga gttgacgatc gagttagcaa aattgaatgt cagtcgtaat
     8341 gaagcagcca aaggatttaa ggatttccat gtggtagatg agcatacaga aatagacttt
     8401 ggtgaaacag tagtgagctt tttccgtacc actcatacga taccagattc agtggggatc
     8461 aatctgaaaa caaaagaagg tagtatcgtt tatacgggag attttaaatt tgaccaaacg
     8521 gctatcccaa tgtatcaaac agattatgca cgattggcgg aaatcggcaa agaaggagtc
     8581 ttagccttac taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc
     8641 caaatagcag atgaagtatt tgatacgatc aaatattggg aagggcgtat catcgtcgct
     8701 tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg atgcagcaga aaaagcacaa
     8761 cgaaaagttg tcttgactgg tcaagatttc gaacgaatta ttcgcacagc gatgcgttta
     8821 gaaaaacttc aattaccaag tgaagatctg cttgttacgt tgaaagacat gaaaaactat
     8881 gatccagaag aactgatcat tttagaaact ggccgaatgg gtgaacccat caagtctctg
     8941 caaaaaatgg caaacggtac acaccgtaat gtcaagattg aagaaggaga tttggtttat
     9001 atcactacta cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga agatatcatt
     9061 tatcgagctg gtggaacagt gaagttgatc tctgagaata tgcgcgtttc tggccatgcc
     9121 aatcctaatg acttgcaatt aatgatcaat ttgatgaaac caacatattt tgtgccagtt
     9181 caaggagagt atcgtgaatt agctgctcat gcagatttgg ctcatgcagt agggatgcca
     9241 tacaaaaata tctatattac tggacgcgga gacgtgctgg aatataagaa caagcgtatg
     9301 agtgttgcag gaactacaag tgcagaaaat gtaatgattg atggattagg cataggtgat
     9361 atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag aagacgggat tttcgtagcg
     9421 gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa aacctcaaat tacttcacgg
     9481 ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag aaagcagcaa tatgattgaa
     9541 acaatcgttg agaaacattt agaaagcaat gattttgagt ggagcaaatt aaagcaagac
     9601 atccgagatc aattaggccg ttacttattt gaacaaacaa aacgccgccc agtgatcttg
     9661 ccagtcatta tggaagctac tcaacgtcga aataaaaaat aaacaaaaag agtaagatga
     9721 acctctctat agagtgctcg tcttgctctt tttttgcgaa aaacaaataa tttttgaata
     9781 cagcatccct cgctttataa taaacgatag aataaaggga gtgaatcaat gatgagtcaa
     9841 acaaaagtcg aacgttattt agaagaacaa aagatcagct atcaaccact tgattttagt
     9901 catgtggtag aagaatcttt cacagaagaa ctacaaaaaa gagggattga tccagctttg
     9961 atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg tgatcatcgc attgattcct
    10021 ttgaacgatc acttagatta caaaaaaacg agacaagtga cgaaagatcg taaagttggt
    10081 tttcctggaa tggattttgt attaatgcat acaggatatc cccacggagc aaacacccct
    10141 attggaataa aactcgcaca tcctgattat tgttttttag cagacagttc tttaaaagaa
    10201 aaagaagaag taattgtgtc aagcggtgaa atcgggagaa gccttcggat aaaagtagcc
    10261 gatttagaaa agatcgtcca tcctatttac gctgatttaa ttagttaagg gacgagaaga
    10321 gaagctaagc agagcagaga cctcaaactt gagattatat ctgcttggta cttggtgcat
    10381 cctgtattat ctgttacaat tgctttgtac atactgtact atcaatccaa tgaaaatata
    10441 tttagcagct atattttttc attgatgtgc aagtgatgaa atcgttttta ataaaaaaga
    10501 tacagagaaa acgcgaggtg taaaatatga aaattacgta tcatggacat tcctgtttaa
    10561 caattgaaat ggaaaatggc caacgcgtga tgattgaccc gtttattaca ggaaatccat
    10621 tgacagactt aactgcagaa gaagtaaaga cagattggtt gctgattact catggtcata
    10681 gtgaccacat aggagatatg attcctattg caaaagaaaa tcaagccaca attatcagta
    10741 ttgttgagat tgctgattat gcgaacagca gaggagtaga ttcattcggt atgaatatcg
    10801 gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt acatgcacag catagttcaa
    10861 gctatgaagt agacggtatc gttcagtata tgggcgagcc tagcggaatt attattcaag
    10921 cagaaggaaa aacgatttat catgcagggg atactgctta tttcagtgat ctaggattgc
    10981 ttgcagaaga attcgatatt gatgtcgctt tcttgccgat tggcgataac tatacgatgg
    11041 ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc caagaaagta gttccaatcc
    11101 attacaatac tttccctgtc attgaacaag atcccgaatc ttttgtagag ttactggaag
    11161 atagtgaagg aaaaataatg agcgtctcgg aaacgctgga agtgtaggca gaaaatggcg
    11221 acaaaacatg atttgatttt agaatatatt gaaagtttac ctgttggcaa tcgaatttct
    11281 gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa cagcgtaccg tgcaatcaaa
    11341 gatgcagaaa atgtcggact agtttcaaca attcaacgag tagggaccat ccgtatcgaa
    11401 cgcaaattaa aaaaacatat tgaacgtttg acatttggcg aggtcgtcag gatcattgaa
    11461 ggggacgttt taggcggggc ggcaggatta gacaaggtct tgaataaatt cgtcatcggc
    11521 gcgatgacta aacatgccat ggaacgctat attacgccag gatcactaat gatcgtcggc
    11581 aaccgggcgg aagtccaaaa attagcattg gaagatggcg ccgcagtatt gatcacagga
    11641 ggattcaata cgactgctga gattgcaaag ctagctgatg atcttgaaat gccgatctta
    11701 cgcacctctt atgatacatt tacagtcgca accatgatca accgagcgtt aagcgaccaa
    11761 ttgatcaaaa aagatatcat gcttgtcagt gatatctata tgccagctga aaagactcat
    11821 tatttgcagc agacagatac gatttctgat tatcagcgtt tgtcagaaga aacgcagcat
    11881 tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg ggattgttac ggcaaaagat
    11941 gttttaggga aatcgccaaa ccagatcatc gatcgggtga tgacgaaaga accaatcagt
    12001 gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa tgatctggga tgggctagaa
    12061 gtcatgccgg tcgtttccga tgacttgaca ttggaaggct tgattacccg gcaagatgta
    12121 atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg cagatacgat ttcggatcag
    12181 atatctggaa atatccatac gattgatact gaccgtgaag gaaatcgttt ggaaaaccct
    12241 aagttcaaat tcgtagtagc accacaaatg gtcaatggcg ttggaaccat ttcttttggc
    12301 gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa tggtgatgaa ccaaaaacga
    12361 aatatcttga ttgaacaagt gaacctacat tatttgcggc tgatccaatt ggaaagcgaa
    12421 ttagacattc gtccacgtgt attggaaatc ggcagacgtt ccgctaaact ggatatcgaa
    12481 gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg tttgtcaagt aatggaacgt
    12541 tcttagaaag caggagtgaa atgacagtac agcaagaaat agtagataca ataaaaaaat
    12601 acgataaaat cattatccac aggcatttgc gccctgatcc tgatgcatta ggatcacaag
    12661 taggattagc cgaactatta cgggaaagtt tcccagaaaa agaaatacgt caagtaggtg
    12721 aaacgattga agggcttcgc tttttagcag aaatgcaaga agttgaagat gtcttctacc
    12781 aaggagcgct agtcatcgtg actgatacag ctaattcacc cagaatcagt gatcaaagat
    12841 atcagttggc agaaaaactg atcaaaatcg accatcatcc caatgatgac ccttatggag
    12901 atcttgtgtg ggtaaataca aaagcaagta gctgtagtga gatgattgca gaatttgctt
    12961 tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc acggttactt tatgcaggaa
    13021 tcgtcggaga tacagggcgc tttttgtatc cctccacgac gtcccgtaca ttagagattg
    13081 cttcgatatt gagaaattat ccatttgatg caagtgcttt gaacagagag attgaacaaa
    13141 tgccgatgaa agtcgctaaa ctttccggtt atctttatca aaatatccag gtggatgaaa
    13201 atggtgcagg aagaataatc cttccgcaag aaattcttga taaatatgga atcgatgatt
    13261 cagagacagc ttcgatcgtt tcacttcctg gagtgattga cgaagtgctg gcttggggga
    13321 tttttgttca gcagccttca gggtattacc gcgtacgttt acgttctaag gggccgatca
    13381 tcaatgaatt ggctaaacgc catcatggag gaggccatcc attagcaagt ggtgcgaatg
    13441 caaaagattt agcagaagta gaggaaatct ataaagagat ccaagatata tgccaatcgt
    13501 ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg tgccttttgt agtaaaaatc
    13561 acaaatgtat cttttcaaga tcttcttttt ccgttacaat agtgctcact aaagatttta
    13621 aaggagaaag aagatgacat taccaaattg cccagaatgt ggctcagagt acacatatga
    13681 agatcgaggc ttgttgattt gcccagaatg cgggaacgaa tggaacaaag aagaacaaga
    13741 aactgtcata gaagacggat tagtggtatt agatgcaaat ggaaaccgtc tgttagaggg
    13801 cgatagcgtg acagtgatta aagacttgaa agtaaaaggt gcgtcaggtg ccattaaaca
    13861 aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat cataatatcg attgcaaggt
    13921 agaaggattt ggaccgatga aactgaaatc ggaatttgtg aaaaagaatt aaagaatatt
    13981 caaggtcttt tcacattctt ttcctactac taatgataaa ataaagtaaa cgaaaaggag
    14041 tgaaacgaaa atgtatggtt atggaatgta tggtttttta gatccgacat ttcttttggt
    14101 cattcttgga ttagttattt ccggaattgc gtcagcgtac gtcaatagta ctttccgaaa
    14161 atacgatcaa gtgagaagca aaaacaatgt gactggtaca caagcagcac aatatatatt
    14221 acaaagtcaa cagataaata atgtcggtgt tcaacaaatt gccggagatc tcacagataa
    14281 ctacaattca ggtaataaga tgttgagttt gtctcaagca actgcacagt ctacttcagt
    14341 tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg gtacaagatg cttcgggtta
    14401 tgtcccattg aagattcgtg cagcacttgt tccagcagct aactttggtt cgatgatttc
    14461 gttccccttg attatggtag gtgtattgct tagctggaac tctacgttaa tcaatatcgg
    14521 gattttcgct ttttctttag cattactttt ccaattggtg acattaccag tcgaatttaa
    14581 cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga ctactgacgg aagaagaagt
    14641 accgatggca agacacgtat tatttgcagc agcactgact tatgttgcag cagcgctttc
    14701 gacattcctg cagctgttgc gcttgatctt attgttcggc ggcaaccgaa gagattaaaa
    14761 taaagaagtt gtgaaatcag cgctttgatc tgagctaata ccgagagtta gctgattgaa
    14821 taccatttat ttggaata
//
LOCUS       DAZKPO010000072        14631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000072 DAZKPO010000000
VERSION     DAZKPO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.074"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(198..764)
                     /locus_tag="ACG8NZ_002204"
     CDS             complement(198..764)
                     /locus_tag="ACG8NZ_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0539856.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(764..1720)
                     /locus_tag="ACG8NZ_002205"
     CDS             complement(764..1720)
                     /locus_tag="ACG8NZ_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0539857.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1720..2376)
                     /locus_tag="ACG8NZ_002206"
     CDS             complement(1720..2376)
                     /locus_tag="ACG8NZ_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0539858.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2746..4764
                     /locus_tag="ACG8NZ_002207"
     CDS             2746..4764
                     /locus_tag="ACG8NZ_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0539859.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4834..5040
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5191..7605
                     /gene="leuS"
                     /locus_tag="ACG8NZ_002208"
     CDS             5191..7605
                     /gene="leuS"
                     /locus_tag="ACG8NZ_002208"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0539860.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7953..9506
                     /locus_tag="ACG8NZ_002209"
     CDS             7953..9506
                     /locus_tag="ACG8NZ_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0539861.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9887..11671)
                     /locus_tag="ACG8NZ_002210"
     CDS             complement(9887..11671)
                     /locus_tag="ACG8NZ_002210"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539862.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11686..13407)
                     /locus_tag="ACG8NZ_002211"
     CDS             complement(11686..13407)
                     /locus_tag="ACG8NZ_002211"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539863.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13507..14268
                     /locus_tag="ACG8NZ_002212"
     CDS             13507..14268
                     /locus_tag="ACG8NZ_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0539864.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4751 a         2705 c         2916 g         4259 t
ORIGIN      
        1 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
       61 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
      121 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
      181 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
      241 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
      301 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
      361 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
      421 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
      481 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
      541 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
      601 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
      661 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
      721 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
      781 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
      841 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
      901 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
      961 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
     1021 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
     1081 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
     1141 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
     1201 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
     1261 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
     1321 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
     1381 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
     1441 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
     1501 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
     1561 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
     1621 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
     1681 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
     1741 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
     1801 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
     1861 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
     1921 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
     1981 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
     2041 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
     2101 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
     2161 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
     2221 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
     2281 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
     2341 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
     2401 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
     2461 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
     2521 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
     2581 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
     2641 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
     2701 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
     2761 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
     2821 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
     2881 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
     2941 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
     3001 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
     3061 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
     3121 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
     3181 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
     3241 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
     3301 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
     3361 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
     3421 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
     3481 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
     3541 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
     3601 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
     3661 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
     3721 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
     3781 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
     3841 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
     3901 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
     3961 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
     4021 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
     4081 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
     4141 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
     4201 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
     4261 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
     4321 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
     4381 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
     4441 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
     4501 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
     4561 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
     4621 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
     4681 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
     4741 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
     4801 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
     4861 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
     4921 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
     4981 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
     5041 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
     5101 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
     5161 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
     5221 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
     5281 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
     5341 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
     5401 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
     5461 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
     5521 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
     5581 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
     5641 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
     5701 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
     5761 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
     5821 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
     5881 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
     5941 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
     6001 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
     6061 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
     6121 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
     6181 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
     6241 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
     6301 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
     6361 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
     6421 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
     6481 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
     6541 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
     6601 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
     6661 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
     6721 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
     6781 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
     6841 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
     6901 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
     6961 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
     7021 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
     7081 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
     7141 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
     7201 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
     7261 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
     7321 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
     7381 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
     7441 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
     7501 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
     7561 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
     7621 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
     7681 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
     7741 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
     7801 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
     7861 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
     7921 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
     7981 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
     8041 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
     8101 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
     8161 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
     8221 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
     8281 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
     8341 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
     8401 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
     8461 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
     8521 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
     8581 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
     8641 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
     8701 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
     8761 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
     8821 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
     8881 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
     8941 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
     9001 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
     9061 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
     9121 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
     9181 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
     9241 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
     9301 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
     9361 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
     9421 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
     9481 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
     9541 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
     9601 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
     9661 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
     9721 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
     9781 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
     9841 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
     9901 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
     9961 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    10021 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    10081 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    10141 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    10201 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    10261 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    10321 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    10381 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    10441 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    10501 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    10561 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    10621 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    10681 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    10741 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    10801 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    10861 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    10921 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    10981 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    11041 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    11101 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    11161 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    11221 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    11281 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    11341 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    11401 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    11461 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    11521 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    11581 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    11641 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    11701 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    11761 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    11821 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    11881 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    11941 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    12001 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    12061 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    12121 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    12181 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    12241 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    12301 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    12361 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    12421 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    12481 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    12541 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    12601 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    12661 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    12721 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    12781 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    12841 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    12901 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    12961 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    13021 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    13081 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    13141 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    13201 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    13261 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    13321 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    13381 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    13441 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    13501 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    13561 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    13621 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    13681 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    13741 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    13801 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    13861 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    13921 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    13981 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    14041 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    14101 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    14161 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    14221 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    14281 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    14341 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    14401 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    14461 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    14521 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    14581 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac a
//
LOCUS       DAZKPO010000073        14549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000073 DAZKPO010000000
VERSION     DAZKPO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.075"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8NZ_002213"
     CDS             4..816
                     /locus_tag="ACG8NZ_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0539865.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8NZ_002214"
     CDS             813..1613
                     /locus_tag="ACG8NZ_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0539866.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8NZ_002215"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8NZ_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0539867.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8NZ_002216"
     CDS             3110..4819
                     /locus_tag="ACG8NZ_002216"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0539868.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8NZ_002217"
     CDS             5031..9389
                     /locus_tag="ACG8NZ_002217"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0539869.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8NZ_002218"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8NZ_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0539870.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8NZ_002219"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8NZ_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0539871.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8NZ_002220"
     CDS             11227..11520
                     /locus_tag="ACG8NZ_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0539872.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8NZ_002221"
     CDS             11517..11828
                     /locus_tag="ACG8NZ_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0539873.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8NZ_002222"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8NZ_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0539874.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8NZ_002223"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8NZ_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0539875.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DAZKPO010000074        13978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000074 DAZKPO010000000
VERSION     DAZKPO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.076"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..461
                     /locus_tag="ACG8NZ_002224"
     CDS             141..461
                     /locus_tag="ACG8NZ_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539876.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="ACG8NZ_002225"
     CDS             913..1113
                     /locus_tag="ACG8NZ_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0539877.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="ACG8NZ_002226"
     CDS             1260..4049
                     /locus_tag="ACG8NZ_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0539878.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="ACG8NZ_002227"
     CDS             4096..4623
                     /locus_tag="ACG8NZ_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0539879.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="ACG8NZ_002228"
     CDS             4644..5834
                     /locus_tag="ACG8NZ_002228"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0539880.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8NZ_002229"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8NZ_002229"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0539881.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7967..8641
                     /locus_tag="ACG8NZ_002230"
     CDS             7967..8641
                     /locus_tag="ACG8NZ_002230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0539882.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8638..8979
                     /locus_tag="ACG8NZ_002231"
     CDS             8638..8979
                     /locus_tag="ACG8NZ_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539883.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9009..9368
                     /locus_tag="ACG8NZ_002232"
     CDS             9009..9368
                     /locus_tag="ACG8NZ_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0539884.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9628..9750
                     /locus_tag="ACG8NZ_002233"
     CDS             9628..9750
                     /locus_tag="ACG8NZ_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539885.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9892..11976
                     /locus_tag="ACG8NZ_002234"
     CDS             9892..11976
                     /locus_tag="ACG8NZ_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0539886.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12287..13753
                     /locus_tag="ACG8NZ_002235"
     CDS             12287..13753
                     /locus_tag="ACG8NZ_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0539887.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4636 a         2430 c         2974 g         3938 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact tcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     5341 tcaatggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggga
     5401 attctgagat catcaatgga atgattgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccag
     5581 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatca catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     6721 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     6901 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacggacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggttgt gcgcctgccg ttcagctccg
     7201 agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt gtgacttgtg
     7261 gccggagagc aacgtttatt taaaaaaaag aactgcgaac tctcgcagtt ctttttttta
     7321 aatacgggat aatggaagtt tccagcagac aagagaagaa agcaaaagaa attgctattt
     7381 gattcacaag gttctataac gaaatcgcaa tcaatcaaac atttacaaag aaggtaattc
     7441 aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat ctagcacctg
     7501 aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag catgcaataa
     7561 tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt atgaccaatc
     7621 tatcccaagc aatctagaag agatattagt aaagggacat ttactagaag tcttagatga
     7681 gcctataaat atatgtttcg atggcgtcat cagtggtctg attgacggaa gcttgggtgt
     7741 gatcgatctc tctggaccaa acaacgaagg aatcaagggg aatccggcac gttaagaaaa
     7801 aaatagcata aaaaactaga cacttgatga gcgttcattc aattgtttga aggaactagg
     7861 ctggtgtcat tttttataca tatattttct ctttcgtctt gatagggctt atgggataat
     7921 ataaggaaga aaatcatcgt tttattcaaa aaggagtttt tgacaaatga aaaaacgtat
     7981 attatttatc ggtgatagca ttacagacgc aggaagatac cgagcagaag caactgatct
     8041 aggtaaaggt tatccacttt tgattgccgg aaaactaatc tcccaatatc caacgatgga
     8101 atttgaatgc atgaaccgtg ggataggagg agacaaaatt gcagatctgc aagtacgctg
     8161 gcaagaagat tgtttagctt tacaacctga ctatgtatcg attttgatcg gaatcaatga
     8221 tacctggcat aatactagcg atcaggctat attcggaact gagaaatctg ctcagcaatt
     8281 tgaatccgtt tatcgaaaat tactaaatga acttcaaaca gcggaaatct gcaacatcat
     8341 cttgatggag ccgtttgtac ttcctataac cgacgaccgt catggctggc gtaaagactt
     8401 agaccctaaa atccaaattg ttcgtaaact tgctaaggaa tatggcgcat tgctggttcc
     8461 tttagatggt atattcaatg ctttagggat tgcttatggt ttttcgaaat atacaagaga
     8521 agatggggtc cacccaacga ttggcggaca tgagatcatt gcacaaagct ggatagatgc
     8581 aattgtcgca gtcgattttt ttagtggaga taaaaatgag cgaaagaaaa gtgaaaaatg
     8641 atggaaataa ggtgggcagt aaaagaagat ttacctcaaa tcaatcagct gattactgat
     8701 catccagaat cattcgtaaa agcaaaaacg gcagctgacc taaatgataa gaataaacta
     8761 ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat ttgcgcaagc ggacgtgcaa
     8821 cagtcagaag aacgattgga tcagcgacca tcaggaaaac taatccaaca cacattagta
     8881 acaaaagaaa atacggaaga aatccaaaaa aaatttgcag aatacttgat gctggaagct
     8941 aaaaaaagaa gaataaaatc aatttttgcc cagttttaat caaaaacagt aatagaagga
     9001 gaaatacaat gagttgttta ggaaatgtca tctggtttat attcggcgga ctaatcggtg
     9061 gtcttagctg gttagcagcg gggatacttt ggtgtatcac gatcataggt atcccaatcg
     9121 gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc ttttggcaaa gaagtaattt
     9181 atcaccagtc tggcatgtca ttgatcgtta atatcatctg gctgatcgtt tcaggcctac
     9241 caattgctct cgcacatgtc gtcagcggca tccttctatg tatcacgatc atagggatac
     9301 cttttgccaa gcaatctttc aaacttgctc gattggcgtt gatgccattc ggtgcagaaa
     9361 ttgtataaaa gagaataata aaggttagat aaagcataga gccaggtggg atacatgtat
     9421 ttcacctgac tctatgcgtt ttcctgtata agttcttcta tgtgactttt acgtctttta
     9481 tttatatggg gttactgttt atggaaagtt gaaataattt tcattaaaga actctttcta
     9541 tatttttgaa agcgtagtgg agcttcgtat atctgggaag gacattttgc agggattgaa
     9601 aaaagatatt gtcctatcga aaaattaatg aatttagaca aattgccagc aacaggcttc
     9661 aaacttagtt gctatcctgt caagatcaag aaggcaagtg cgggttggat aagagcagtg
     9721 gcaatgatcg aagagaaaaa gaaagaataa gccaaaacat ctgtatggaa gaaattttcc
     9781 gatgcagatg tttttcattt tttctttcgg ataccttgac atatgaataa ccattcattt
     9841 ataattagtt atgaagagat attcatatgt tgaggcgagg aggtcattaa aatgaaaagt
     9901 tacagattag aaggtttaga ttgtgcgaac tgtgcaatga aaatcgaaaa aggagttcag
     9961 aaaatcaatg gagtcaagga agcaacggta aacttcactt ctggaaaact aacgattgac
    10021 gcagaggaag atcatttggc tacgattgaa caagaaacaa aaaaagtagt gaaggaatta
    10081 gaaccagatg tcaaagtaac agaaatagat aaggaaaaag tatctgaaca tggaaatgaa
    10141 aaaaagagaa atacgctgtt ccggatttta ttttcccttg caggtatcgc tttattgcta
    10201 ttatttgatt ttaatgaacc aattcgcttg atagggtatt tattgattta tctgctgata
    10261 ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca aaggaaaaat ctttgatgag
    10321 aattttctta tgtctgttgc tacgattggc gctatgttga tcggtgaata tccagaagcc
    10381 gtagcagtga tgctatttta tcaaatcggc gagtatttcc aaggattggc agtcagtcat
    10441 tcaagaaaat cgattcgcga attgatggcg atccgtccag aaacagctca cgtgcagacg
    10501 gcggaaggat tgatgaccgt gaaccctgaa gacgtgctga tcggtcagtt tgtacttgtg
    10561 aagcctggtg aacgtgtacc gttagatgga acgatcatcg aaggagaatc attagtcgat
    10621 acgtcagcac ttacaggaga atcagtccct aaatctgtat atgttggtga aacagtatta
    10681 agcggattta tcaataaaaa taaaccattt ttagttcaag tagaaaaatc ttatgaaaat
    10741 tctactatca gcaaattatt agaattagta gaaaatgcca gcagtaaaaa agcaccagct
    10801 gaaaatttca ttactaaatt cgcgcgctat tacacaccag tcgttgtagg attggccgtt
    10861 ttattagctg ttttacctcc acttgttgtt tctagagcgg cgttttctga atggatctat
    10921 cgagcattga catttttagt gatctcatgt ccatgtgcat tagtgatttc cgtgccactt
    10981 agtttctttg gcggtatcgg aggagcaagt aaaatcggtg tattagtaaa aggttcaaat
    11041 tacttggagt tgcttgcaca aactgagaca gtggtattcg ataaaactgg cacattaacg
    11101 aaaggtgact tttcgatcca gacgatcgat ccaacaggaa ttgatccaaa gaaatttatg
    11161 caatatgttg caagtgcaga acagttttct acacatccga ttgctcaatc tgttttagaa
    11221 ggctacgcag gtccgctttt accaacagct aatatccagg aatttgcagg tgaagggatc
    11281 ttagctgaag ttgatggcaa acaagtatta gtaggtaatc acaaattgat ggaaagattt
    11341 gaaatctctt ttccttcaag tcaagaaatc ggtacattgc tttatctagc gatcgatcaa
    11401 agttacagtg gttatcttgt tattgcagat accttgaaag aagatgccgt agacgcattg
    11461 gttcaattaa aacaggctgg tgtgaaaaac acagtcatgt tgacgggcga ctcgaaaaaa
    11521 attgctgatc atatcgggaa gcaagtaggc gtggacaaga tatatagcga attgcttcca
    11581 gaagataaag tgcagcggtt agaggaaatc ttacaaagta acaataaaaa gaccgcattt
    11641 gtcggagatg gaatcaacga tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg
    11701 ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa
    11761 ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa cattgaaaat cgtcaaacag
    11821 aacattgtct ttgctattgg gataaaaatt ttggtacttt ccttaggtgc ttttggtttt
    11881 gcatcaatgg gagatgcagt atttgcagat gttggggtga cagtattagc tgttttgaat
    11941 gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt
    12001 cctctttttt ttatatcctt ttcttaatga aaaatgtaaa gaaaatagat aggattataa
    12061 aataactaat gtttttaatt ttgcagaaaa ttcaaaatag cttgaaattc aataaggtca
    12121 atgaaataaa agaaaataac ttattttttt gatggaaaaa atatgaaaaa acgatgtgta
    12181 ttaaataaaa gaatacacac ataaaataac actgtcttgt aagtttgtta aaaattgaat
    12241 atgattacat ctggataaca aatttttatc ataaaggaga gaacgaatga acaacaaaca
    12301 attacgctgg tttactgttg cgttaattgc atttaacatg gtttggggca tggggaatgt
    12361 agttaataac tatgctcagc aaggagtatc agttgtcacc tcttggctat tgattttggc
    12421 catttatttt attccatacg cattgatcgt tggacaatta ggatcagcct tcaaggatag
    12481 taaaggaggc gtcagttcat gggtcgaaaa tacgacatct aataaacgac tggcatacta
    12541 tgccgcttgg acttactggg tcgtccatat tccttatttg gcacaaaagc ctcaagcaat
    12601 tctgattgct ggtggttggg cagtccaagg aaatggaaat atcgtaaata cgatgtcagt
    12661 acaaatggta gcagttattt cacttattat atttcttgcc tttttatatt tatcaacaaa
    12721 aggactttct actttgaaag tgattggcgg actggccgga acagcgatgt tcgtcatgtc
    12781 gatcttattt attcttttag ctgtaacggc accgttcatc aatccaacga tggaatttgc
    12841 cacaccacat atgaatcaag tgaaaacata tattccaaca tttgatttca gttatttcac
    12901 aaccgtttcg atgctagtat ttgcagttgg aggggcggaa aaaatatccc cttatgtcaa
    12961 ctctacaaga aatcctgcaa aggaatttcc taaagggatg atcttcttag cagcaatggt
    13021 agggatttgt gctgtattag gctcagtcgc aatgggtatg ctgtttgcta gcgataatat
    13081 ccctaaagac ttgatggcaa atggggcata cagtgctttt caaattttag gtaattacta
    13141 tggtatcggt aactcattga tgatcattta cgcattgaca aacatgattg gacaaatctc
    13201 cgcattagcc ttttcaattg atgcaccact aaaaatcttg ttagcagatg cggatccaga
    13261 atttgtacca aactggctac gtaaacgtaa tcgtaaaggt acattggtga atggttatct
    13321 tctgacaggt atactagtag gaatcattat tttgttgccg atctttggta tcaaagatat
    13381 gaatgaatta gtaaaatggt tgacgaactt gaactcagtt gtcatgccaa tgcgttacct
    13441 atgggtgttc ttggcatata tgatgttgaa taaagcttat aagaaattca attctgaata
    13501 taaatttgtt aaaaatccaa aaatcggttt tatctttggt gcttggtgct ttgccttcac
    13561 tgcgtttgca tgtatcctag gaatggtgcc aaaagtagat tatactgctg atcctaaagc
    13621 atggtggttc caattattca cgaatatcat cacaccaatt gtcttgatct tattaggtat
    13681 gatcttgcca gcaattgctc gtcgagacaa aaacaaagaa attactgaac cagaaacagt
    13741 ggttcagcct taaaatagaa atacttgtta caagaggttg tgacagaagc tacttctgga
    13801 acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    13861 caaaaatagc tcctcatatt ttgccgtttt ttgagctaat gctgaaggtc agctgattga
    13921 acaccattta ttcgaaacaa gggactttga cacgactttt gtcacagtcc cttgtttt
//
LOCUS       DAZKPO010000075        13820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000075 DAZKPO010000000
VERSION     DAZKPO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.077"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..2171)
                     /gene="tkt"
                     /locus_tag="ACG8NZ_002236"
     CDS             complement(174..2171)
                     /gene="tkt"
                     /locus_tag="ACG8NZ_002236"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0539888.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2273..2524)
                     /locus_tag="ACG8NZ_002237"
     CDS             complement(2273..2524)
                     /locus_tag="ACG8NZ_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0539889.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2671..3294
                     /gene="lexA"
                     /locus_tag="ACG8NZ_002238"
     CDS             2671..3294
                     /gene="lexA"
                     /locus_tag="ACG8NZ_002238"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0539890.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3474..3692
                     /locus_tag="ACG8NZ_002239"
     CDS             3474..3692
                     /locus_tag="ACG8NZ_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539891.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3981..4493)
                     /locus_tag="ACG8NZ_002240"
     CDS             complement(3981..4493)
                     /locus_tag="ACG8NZ_002240"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0539892.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4507..6804)
                     /gene="recJ"
                     /locus_tag="ACG8NZ_002241"
     CDS             complement(4507..6804)
                     /gene="recJ"
                     /locus_tag="ACG8NZ_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0539893.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6946..7389)
                     /locus_tag="ACG8NZ_002242"
     CDS             complement(6946..7389)
                     /locus_tag="ACG8NZ_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0539894.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7390..8178)
                     /locus_tag="ACG8NZ_002243"
     CDS             complement(7390..8178)
                     /locus_tag="ACG8NZ_002243"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0539895.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8175..9116)
                     /gene="rnz"
                     /locus_tag="ACG8NZ_002244"
     CDS             complement(8175..9116)
                     /gene="rnz"
                     /locus_tag="ACG8NZ_002244"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0539896.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9293..9880)
                     /locus_tag="ACG8NZ_002245"
     CDS             complement(9293..9880)
                     /locus_tag="ACG8NZ_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0539897.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10101..11414)
                     /gene="obgE"
                     /locus_tag="ACG8NZ_002246"
     CDS             complement(10101..11414)
                     /gene="obgE"
                     /locus_tag="ACG8NZ_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0539898.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11702..12712
                     /gene="gap"
                     /locus_tag="ACG8NZ_002247"
     CDS             11702..12712
                     /gene="gap"
                     /locus_tag="ACG8NZ_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0539899.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12797..13732)
                     /locus_tag="ACG8NZ_002248"
     CDS             complement(12797..13732)
                     /locus_tag="ACG8NZ_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0539900.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3815 a         3097 c         2435 g         4473 t
ORIGIN      
        1 acaaataata ttctcaagaa acacaaacta atccgaagaa ttctttgttt tgtcatttaa
       61 atgtaacata gccgaaaacg aaaagtaata ttgatttatt cttctaacac ataaagaaca
      121 gctgtctcca acttgcagac agctgttctt atcagtcaaa caattgcttg gatctatgat
      181 agttgattgt acgtattcac gacattttct actgtaaatc caaattcttc taaaatttta
      241 gtgccagggg cagatgcacc gaaatgatcg attgtaatcg ttgcgccctc tgttcctacg
      301 tagcgttccc atccaaatga tgcagcagct tcgatagcca cacgtttctt cactgattta
      361 ggaagtacac tttctttata ttcagaagat tgtttttcaa agaggtcaaa gcttggcata
      421 gaaacaacag aaacatcttt gcctttttca gccaattctt tttgagcttt cacagccaaa
      481 tcaacttcag aacctgttgc aatcaaaata ccttctggtg tttctccttg tgatggagaa
      541 agaacatacg cgcctttttt caccatttca tctgctactt cttttgtaga aggcaatact
      601 ggaaggtttt gacgactcaa tacaaggata gtaggcgcat ctgttgtctc catcgctact
      661 ttccaagcag cacgtgtttc atttccgtct gccggacgaa tcacttgaac accaggcatg
      721 cagcgaacag atgctagttg ttcaatcggt tcgtgcgttg gtccgtcttc tccgacagca
      781 accgagtcat gtgttaagac gtaaacaaca ggagtatgtt ggattgctgc taaacgaact
      841 gctggtctta gataatcgac aaagacaaag aatgttccac cgtagatacg agttccgcca
      901 tgaagttgga taccattcat agccgaagcc attgcaaatt cgcggacacc gaaccagatg
      961 ttgcgtcctt cataatgttc aggagtgaaa tctttatctg cagccaccat tgtattattg
     1021 gaacctgaca agtccgctga accaccccag aagcttggta tagctttcga taattcttgg
     1081 atgacttctt tacttgaaac acggcttgct tgactgcttc caacttcata agttggcaat
     1141 tctgcatccc agttttctgg tagtttgcca tcaaaagcat cttcaaattg ttgtgctaat
     1201 tcaggataag cttttttata attggcaaac atttcattcc atgcatcttc agctttttgt
     1261 ccttcttcta tcatcgtttg atggaagcgg gcagctactt cttctggaac agtgaaatct
     1321 ggatactccc aaccataaac ttctttagcc atcttgatgc catcttcacc aagaggagca
     1381 ccatgaacag cagatgttcc ttctttagga gcaccatagc cgatgactgt ttttacttcg
     1441 atcaatgttg gtttgtctgt ttcagctttt gcttcttcaa tcgctttaga gattgcttct
     1501 aggtcatttc cgtctttcac taagatatgt tgccagccat atgcttcata gcgagcacca
     1561 acgttttctg tgaaagcttt tgaagttgga ccgtctagtg aaatatcgtt tgaatcgtat
     1621 aaaacgatca atttccctag tttcatatga cctgccatcg aacttgcttc ttgagatacc
     1681 ccttccatca agtctccgtc accgcatatt gcatacgtat agtgatccat cacattaaag
     1741 ttttctttgt tatacgttgc tgcaaggtgc gcttctgcca ttgccatacc gacagccatt
     1801 gcaatccctt gtcctaatgg ccctgtagtg gcttctacac catctgtatg atgtacttca
     1861 ggatgtcctg gtgttttaga atcccattga cggaattgtt tcaaatcatc gattgtcact
     1921 tgatagccag ctaaatgaag caagctatac aacattgctg acccatgtcc tgccgaaaga
     1981 acgaaacggt cgcggtcagc ccagtttttt gatgtcgttg gatttacttt caggtgtttt
     2041 gtccataacg catatgccat aggtgctgcg cccatcggca atccaggatg tcctgagttt
     2101 gctttttgga ctgcttcaat acttaatgta cgaattgtgt taacgccaag ttggtcaatt
     2161 ttgtcaaaca aaataacccc tcctattaat ttaggttttt tttaccttta tttcttacac
     2221 tttcattcta taagatgtca cacgaaaaag caatcttttt cagtattttt tactaaaagt
     2281 tgtcatttcg gttgtgtaag ccttttttct tttggatttc ttttaatttt tccggggtca
     2341 cgtcattgcc ctcaggatcg acgactttca ttccttcgat atgatgacgc ataccggaac
     2401 gaaaagcttc caaatactct ttgcgcaagt tgatttgttc ttgttcttcg gcttttgaga
     2461 gaccattttc cttcttttta cgtgcaagtt cattgatccg ctctattttt tcaggtgaca
     2521 gcatgctcag ccctcctttt actctccttc atattatata aaaactgaaa aaaaaacaac
     2581 tagataaact aaataaaata cgaacattcg tttgatttag cgctttttat atggtactat
     2641 ttatttatca agtgaaagaa ggtgtacctt gtggcccgac aaactgaatc aagacaaatc
     2701 gaagtcttac gatatatcca tgaacaagta acccaaaaag gatacccgcc gactgtccgt
     2761 gaaatcgggg aagctgttca gctttcttct acttctactg ttcacggtca tcttgcacgg
     2821 cttgaaaaaa aagggtttat ccaaagagat ccgacgaaac ctcgtgcaat cgagttgaca
     2881 aaacaaggtt tagaaaagat tgggattcgt ccaacaacta ttccagtact tggcgtcgta
     2941 acagctggag aacccatttt agcagtagag gatgcttcag acttctttcc aatccctcct
     3001 aatctttctt ccgaagaagg cagtctcttt atgctgacaa ttcgtggaga tagtatgatc
     3061 aatgctggga tattggatgg tgacaacgtg atcgttcgga agcaagaaac tgctcaaaat
     3121 ggcgatattg tcattgcaat gactgccgag gacgaagcga cttgcaaacg attctataaa
     3181 gaaaaaaact ttttccgtct gcagcctgaa aacgacttgc tcgaaccaat catcttagat
     3241 caagtatcta tattaggacg agtagtagga ctttatcgta gtcatatcta ttaattgcta
     3301 ctaattaaaa gaaaactttt taaaaagagt ttgagacgtt ggtctcaaac tcttttttcg
     3361 cccgggttct tcttctttca aatttttatc acttccctct ttacaaaccg aacaaatatt
     3421 cgtatactaa atatacgaac aattgttcgc ataaattaaa gaggtgagaa ttcatgaaat
     3481 caatgagacg tcttggctta gtattaactg ttttgttttt aggtattatt attggtaatt
     3541 tcggcatacg tccagcttta cttgtgtttg ttccgttatt tactttgtgg ttcatgttgt
     3601 gggatgaaaa gaagtaccgt caatctgagc ggcgcagaat tcatgaagat catttctatc
     3661 atgagcccta ctacagatcc aggcaatcat aataaggatt gactgctaat tattcaaaag
     3721 acaagaagtt aaacttttac gggggtgtga caaaaatcgg tcacatcctt tttttcgaat
     3781 aaacggtgtt ctaaagccag tttttcggta ttaagtcaaa ttgcggaaaa agtgattttt
     3841 taaacttcta ctggtctttg cttgtttcca tttataaaaa aatagcgacc ctcaaaagtc
     3901 agatagagaa tctgactttt gagggtcgct ttaaaatcgc ttctttaaga ataacatcaa
     3961 gaatagcttc ttttttctgc ttaatattcc attaatgtaa tgatgtcata tcctttgatt
     4021 ttgtcgcgtc catggagatc catcaattcg atcaaaaatg cgcagcctac gactacaccg
     4081 cctaattgtt cgatcagttc gatcgtcgct ttgattgttc ctccagtagc tagcaaatcg
     4141 tcacaaatca gcacacgttg acctggttta atggcatcct tatgcaatgt caatgtatct
     4201 gttccatatt ctaatccata agtcacttcg atggtttctc gaggtaactt cccttttttg
     4261 cggactgggg caaaaccgac tcctaactca taagcaacag gacatcctac gatgaatcca
     4321 cgggcttctg gcccaactac catatcaatt cgtttttctt ttgcataatt tacgatttgt
     4381 ttagtagctt cccgataagc gtcaccatct gccatcagtg gggaaatatc tcgaaacaca
     4441 attccttttg acggatagtc gggaatactg gcaatataat cttttaaatc cactctttgt
     4501 ttcctcctag gttgttaacc atttctttat ggtaggaata tcgctcaaaa gcaaaaattc
     4561 ctcaactttg attttattga gccgtcgctg gtataatctg ctctcagtca aaggatgact
     4621 ttctgggcta gcgacttttt gtaaaacacc gttctctatt gtaacaaatt tcaattcaaa
     4681 aaacacctgt atcataaaaa ttaataattt ttcagggata cgcaaatatt gagctattgc
     4741 ttttatttta tgacgtatat caattcgttc ttgagaatgg atcaatttaa acaattttgc
     4801 gtactgttct cttgtgccta ccccattcaa ataggcctca tcaggtgaaa tccccatcag
     4861 ataaacacga ttgaatgaat aagaccgaaa aacttctttc agatccgcta aatcatctgg
     4921 acaatccata acaactagag agtttggttt tcttttttca attattgcct ttacagaatc
     4981 tagtccttcg aaaacaatcg cagcttcctg gataatctca ggcacgaatt tcaacgtcga
     5041 agaatcaaat gctaaataaa ggctaccttc cgacgttcgg ctctgttcac ggaaacgttt
     5101 tgcccgccaa tcaaatactt gcagaccacc cacagaaaaa tcagatacca tcagctgcgg
     5161 tttcttacgc ccgttccatt cattgatcga aagctttcct accacatcta cctgattatt
     5221 cagcagttct tcttcttgag taccaaaacc aaaagcgacc gcatcaagct gactcgatgc
     5281 atcggaaagg acaaatttca gatgctgttg gtttgctccg attcttttga tattttctgc
     5341 ttggacttgt cgaaataaaa actgaggcaa aggattatcc gtaccaaatg gcgccaatag
     5401 tttcagctca tcaattcgct ctactgttac ttcatttggc agcaatactt catcaattct
     5461 caactcaggc cctctcatca agtcaatttg atgatcgact atatattggt tcatcttctc
     5521 ctgtagtata gtgacatttt cacttggcat cgttaaaccg actgctgcat gatgtcctcc
     5581 gaagtaagta aataaatctc gcatctggtc aagcatttca aaaagattca aggcttcaat
     5641 actacgtcca gatcctttag ctgatccatc ttcttttaaa gtaaggacaa gcgttggttt
     5701 acccgtttca ttcattattt tcccagcaac aatgcccaaa acgccttcat gccatcctcg
     5761 gtttaccaat agatggatat gattctcctt attgaccatc gctaatgctt cttgtgttat
     5821 ctgctctacg attgcttttc tttcttcatt gatctcattc aatttttttg cttgaacgga
     5881 tgcttcctct tcatcaaacg tcgcaagtaa cgagacggca ggacgtggat cgcccatccg
     5941 accgattgca tttagtctgg gagcaatcga aaaaccgatc gttgtttcgt ctgcgtccct
     6001 catttttacg ccgctttctt caaatagcgc ctgaagtcct atccgttctg aatgatgaat
     6061 ggcatcgatt cctaatgaaa caaggattcg attctcatca gtcaatgaaa ctaaatcggc
     6121 gatcgttcct atcgctacta aatccaaaaa ttctgcaggc ggttcttcca ataatgcaga
     6181 agctactttg aatgctactc cgactcctgc caaatctgga aatggatagt gccccgctgg
     6241 atgacgaggg tgaacaatcg cataagcgtc tggtaaattc tcaggtagtt cgtgatgatc
     6301 tgtaatgatc acgtctacac cagcttgctg agcgtatgct accgcatcat ttcctgctac
     6361 cccgttatca acagtgatga tcaactggat tccttcttcg atcttttctt ggtataccga
     6421 ttggtttggt ccatacccat gctcaaatcg atttggcagg aatacttcta cttctgcacc
     6481 aagtaattcc agtgtttctt tcattaccgt tgcactggtg atcccatctg cgtcataatc
     6541 accgtaaacg aggatttttt ctccactgat tacagcttct tggattcggc tgattgcttt
     6601 atccatatcg tgtatcagat acggatcatg taattcttgt tcctttggat gtaaaaatcg
     6661 gttgatatcc ttttcctcat ggtaacctct ttgccacagt aacttaccga caaatggaga
     6721 taatttcagt tcacggatca tttggagaaa ggattcagaa ggctctgtct cgcttaactt
     6781 ccaatcaaaa tttgcttgtc tcactttctc actcctaact tagttattat agcaaaggaa
     6841 atcagcaaaa aaaagcagaa agaaagaata aaaacagatc agatccgcta gacagatgtt
     6901 cagcaggacc tgatcttttg tacgaccatt tatgaaatat ccctattagt caaaataatt
     6961 gaagctttgt ttgggcgatt gagttttttg ttcttccggc gctttcaatt gactgatttg
     7021 tgcggttttc gcttgtaact ccgcctgata agcagcttcc aactccgcac gttcgtttct
     7081 aaactcgcgt tgctgttctt cttttgcttc ttcaattttc ttttgaagtt ctgtttgata
     7141 ttctgacatt tcctgagtca gctgtttgat ttgctttttt tgctgccata gtgttgccga
     7201 agcagtcaaa aatacaatca gtgcaccaat cagcgcagaa ccgatgatca ctaagatcaa
     7261 tggcgcagaa aaggaagtga agccaaaact tacaggaaca tttttattgt tcaatacagc
     7321 aaaaagaaca ataatcaaaa ctaaaacaaa tccaagaatc acattgcttt gctttttcaa
     7381 ttccttcacc tactttttgt taaaaattcc gctggctaaa taatcaccaa tcgttggaaa
     7441 cagcgtataa aattttgatg caacttctaa aataaacggt tgattgattt cccgttttgg
     7501 atgtctcata ttgcggacga tcatttttgc caacttggca ggttctaaaa cgatttgtcc
     7561 gactttttcc aaatagcttc cgcttggatc agccttatcg aagaaatttg tctcgattgg
     7621 acctggattc actgtcgtaa cagctacgcc aagtggtttc aattctaatc gcaaagcgtt
     7681 ggagaagcca aggactgcaa atttcgtggc agaataaacc gttgattttg cagttgccat
     7741 ttttccagcc atagaggcga tattaatgat atgaccttgt cttctttctg ccatatcgat
     7801 cgcaaatttt tgtgtcaaaa ccatcattcc taagatgttg acttcaaaca tatcgtatgc
     7861 ttttccaagg tcgaatgtaa ggaagtcttc gaaaatccca aaacccgcat tgtttaccag
     7921 aatatcgacg cgcccgactt cttccatcac cttttcatat aattcgtcca cgctttcagg
     7981 attgctgacg tctaccttga aagcataagc ctctttgccg ctcaagcgtc tgcactcgtc
     8041 tctgacacct tctacaaatg ccattcttcg tgcgcaactt ataacgattg ctccttgctt
     8101 tgctgcttca taacagattt gtgcgcccag accgcctgaa ctacctgtta ctaaaacaac
     8161 tttatcttct aaattcatgc ctcttcctcc tcgcgaaatg gaatctcaat gctatcaaaa
     8221 tctttcataa tcgtcgtgtt ttcaaacact tcttgtgctt ctttttcaag ctctaaagct
     8281 gcttttccta aatatctcgc actgatatga gtaagtagca gccgttttgc ctgcgcttct
     8341 tttgcaactt cagcagcttg atgagtcgtg gaatggaaat agttgtgcgc cattcttgct
     8401 tcatctttat taaatgtgct ttcatgtacc agaatatcgc tattttccgc aagcacaacg
     8461 gaattatgtg tttttctcgt atcgcctaaa atagtcacga ttcgtccttt tttatctgga
     8521 cctacgaaat cttttccatt gatcgtccgc ccatcttcta gttggatcgt ttcaccttgc
     8581 ttcagtttgc cgtacagtgg tccagctgga atacctaatt cttttagacg atccacctgc
     8641 aattctcctt tatgatcatg ttcaaccaca cgataaccaa aactgtcgat cccatgattt
     8701 agtttttttg tataaacaga aaattgctga tcagtaaaaa tcggggctgt ttcatttagc
     8761 tcaatgaatt tcagcggata acttaaacgc gtttgcgaaa tacctaaaga gacttttatg
     8821 tattcctcta tcccttttgg cccgtaaatc tctaatggcg tatccccacc ttgaaatgac
     8881 cggctgctga tgagcccggg aagtccaaaa atatgatcgc cgtgtaaatg tgtaatgaaa
     8941 attttaccga tttttctcgg acgaatagtc gttcgcaaaa tctgcatttg cgtgccttcg
     9001 ccacagtcga acaaccaaac ttcattgcgc tcgtctaaaa gtttcaatgc tatacttgtc
     9061 acatttctat gttttgcagg aacacctgca cctgttccta aaaattgtaa ttccataatc
     9121 tacctgactt tctttaagtt cctctttaat tttagggtaa aaagaaggga taatcaaccg
     9181 taagtcgaga atgggttcaa attaaaaata acgttctaaa gcgatagcgt tttctttgaa
     9241 aattactata aaaacagcag cgaacccgtt tgagatcctc tgctgttttt tattaatttg
     9301 aagatccaga actttcattg tcgttggaag tttgcggatg tttgtccttc caagtctttt
     9361 gtgcagcttc tttcgcatga ttttcagctt ctttcgcatc atgcacatct ttttcgacat
     9421 cttcataatc caagcgagta atttctccgc ctaattccat cattaccaat tgattcagtg
     9481 ggcgattgtc ctcttcatca gcaatcaaga tcagtcctgt agctccttca ccgattttat
     9541 ttcctacaaa gtcgaacacg gtctgggcat cttttatttc tttggcatct tgtgtcgcac
     9601 cgatcattcc tccggcaaac catcctaata aaatccctag cggaccacct aatacaccaa
     9661 ccaacattcc gattaatcca cccgtagaag atttgttatt accggtaaag tcgataaaat
     9721 cttctatttt gaattgatgt gtcccattag gatcatggct gacaactgcc atctgttctc
     9781 ctttaatcga acgaacagca tgtaactttt tgatttctga aaatgcctga tatgcctggc
     9841 tttctacttg aaaatttaaa atgattactc gtttgctcat taaaaacgcc cctttcatac
     9901 cttcttgtta tttattttac ctttaattga aaaataagaa aagaaaagca ctcttttttt
     9961 ccaataaaat agggctgtga caaaagtcgt atcacagctc ctttttccga ataaacggcg
    10021 ttcttgaagc tgatcacttc tgtcacaacc tcccttaaat ctgaatctat attttttggt
    10081 atttgttttt atttgcttgc ctattcgaca aattcaaatt caaactcacc gatacgtaca
    10141 aggtcgccat ctttcgcacc gcgtgcccga agagcttcat ccacacccat accacgtaat
    10201 tgacgagcaa aacgcatcac tgtttcatca tgatcgaaat tcgtcatttg gaataatttt
    10261 tcgatcttct caccagataa gatccacgtt gcatctgaat cacgatcgat ttggaactca
    10321 ggaccttctg gattgaatcc atagtgtaca gtttcttctt ccagttcttc ttcgtacaat
    10381 gggaattctg gcgttacttc taacaaatct gctgtagcat tcaataacgc gtccaatcct
    10441 tgtcgagtca ctccagaaat tggaaatact gggatgtcat cagcaaattc atcttctttt
    10501 tccttgttca gctgttcttt gaatttcact agattttccg gtgcatccgg catatccatt
    10561 ttgtttgcca cgatgatttg cggacgctct agcaagcgta agttataagt agataattct
    10621 ttgttgatgg caagatagtc ttcataagga tctcgtcctt ccatcccact catatcgatc
    10681 acatgtaaaa ttactcgtgt tctttcgata tgacgaagga attgtgtacc cagcccaaca
    10741 ccttgcgaag ctccttcaat caagcctggc aagtctgctg cggcaaaact tctgccatca
    10801 cttgtagtaa ccattcctag attaggtacg agcgtcgtga aatgataagc gccgattttt
    10861 ggacgagcag atgaaataac ggatagtaaa gtcgatttcc cgacagatgg aaaacctaca
    10921 agtccaacgt ctgccaatac tttcaattct agttcgattt ttcgctcttg tcctggttca
    10981 ccgttttcag caatttccgg tgcaggattc cgaggagagg caaaacggat attccctcgt
    11041 ccaccgcgtc caccttttgc cacaactagt gtttgaccat tttcaattaa atctcctaaa
    11101 agagcaccag tctccgcatc tcggacagtg gttccttgtg gtactttaac ataggtatct
    11161 tctgaaccac gaccgtgcat ccctttactc atcccatttt cacctggctg cgctttgaaa
    11221 tggcgattga accgaaaatc catcaatgta cgtaaccctt catcgacgat caagatgaca
    11281 tcgccgccac gaccaccgtc tccgccagcc ggtccgccgt caggaacgta tttttcgcga
    11341 cgaaatgcaa ccattccgtc tccgcccttg ccagctttca catcgatcgt tacttgatct
    11401 aaaaacatgg acataattaa gtcctcctat tattcattta tttatttgac tgcctgaatt
    11461 tccacaataa acaaaagtct tacttatttt atcggtgaaa tctgattttg tctatcattt
    11521 cagcaaaaag attgtttacc ttcttctttt tttatcattt ggtaaggttt ccaacctttt
    11581 ttctgctacc aagtataatt ttttgacaaa atattctttg cgtagcagaa taacagtgta
    11641 ttaattgtat taattgagga tgccgtttca cagcacccct aataaaggag gaacttttga
    11701 tatgacagta aaagtaggta ttaacggatt tgggcgtatt ggccgtctgg ctttccgccg
    11761 catcaaagaa gtctcagatg atattgaagt agtagcaatc aacgacttaa caagtccaac
    11821 tatgttagca caattattgc aatttgactc tactcatggt acttatcctg gcacagttac
    11881 tgcaacagaa gactcaatcg tagtggatgg cgaagcaact cgtgtttatg cagaacccga
    11941 cgcaagcaaa atcccttggg caaaagaaaa cggtgtagat atcgtactag aatgtactgg
    12001 tttttataca tctgaagaaa aagcaaaagc gcatttagat gctggtgtca aacgagtagt
    12061 tatttctgcc ccagctggtg ctatgaaaac catcgtctat aacgtcaatg atgatacatt
    12121 agatgaaaac gacaaaatca tctcagcagg ttcatgtaca acaaactgtt tagcacctat
    12181 ggcttatttc ttaaataatg aatttgggat cgaagttggt acaatgacaa cagttcatgc
    12241 ctacacttcc acacaaatgc tcttagatgg acctgtaaaa ggcggtaatt tgcgtgctgc
    12301 tcgttcagct gctgacaata ctatcccaca ttctactggt gctgcgaaag caatcggctt
    12361 agttatccct gagctacaag gaaaattaca aggacacgca caacgtgttc cagtcgttga
    12421 tggttcatta actgaattgg tttcagtatt gaaaacaaaa gtaactgctg atcaagtaaa
    12481 cgaagcaatc aaaaaacaca cagttgacaa tccttcattc gggtatgatg accgtcaaat
    12541 cgtttctagc gatgtcattg gaacaactca aggttcgatc tttgacccaa cgcaaacaga
    12601 agtaacaact gctggtgatt tccaacttgt taaaactgtt gcatggtatg acaatgagta
    12661 tggatttact tgccaaatga ttcgcttact agaaaaattc gctaacttgt aatttgatag
    12721 atcaatcgat aaaagaagcc tttgactgga tccagtcaaa ggcttctttc ttttcatccc
    12781 ctacttttaa actcgttcaa tgaacatatt ggcttaaaaa ttgttggact acttgctgat
    12841 atttttcagg atctgtcttg tatgcttccg catgctcggc tccttttaca acatactttt
    12901 cttttggtcc cttcgttgca cgatagacct tatctaacat ttcaaatggt acaaatgtat
    12961 cttcgtctcc atgtataaag agcattgggc gctggttttt cttcaattgt tccactgcat
    13021 ttgcttcacc gaaaaagtag ccagcacgga cttttgtcac tagactcgtt acttgcataa
    13081 gcggaaatgc tggaagacta aacatatcgt ctagctgata agcaagctca tcatgaacgg
    13141 aagaataacc gcaatcttca atgattgctt tcacattatc tggtagtttt tctccgctag
    13201 tcatcataac tgttgctgct cccatgctga caccgtatag aacgatttct tgcgattttc
    13261 cattgttttc tagcactttg tttatccact gtacatagtc tttacgctct ggccatccaa
    13321 atccaatgta atccccttcg ctttttccat gtcctcttgc atcaggaaca aggacattgt
    13381 aacccatatc atgaaacatc tttgcgtaat tggtcatcgt ttcagcgttt cccatatagc
    13441 catgagccac aagagctgtt ttttcacttt tcgtttcagc aggaagataa atcgctgata
    13501 atttcagacc gtcttttgaa gtgatggtcc aatcgcttcg gttttctttt gaactaaacc
    13561 atttatgtgc atccgtttct ttggatgtct gtgttgtacc aggtgtatct tcgtctaaaa
    13621 agtctttctc agaaggcaca acagcataat tataaaaata attccctgca aacactaacg
    13681 caacaatcag agctaaagcg acgcctaaaa ggattttcat ccaaagtttc ataaatcatc
    13741 catctcattt ctcttttttc atataacctt gaattagtat aatcaaagaa tctctactat
    13801 gcaatgttca tttttataac
//
LOCUS       DAZKPO010000076        13724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000076 DAZKPO010000000
VERSION     DAZKPO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.078"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG8NZ_002249"
     CDS             complement(45..1364)
                     /locus_tag="ACG8NZ_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0539901.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG8NZ_002250"
     CDS             complement(1361..2014)
                     /locus_tag="ACG8NZ_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0539902.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="ACG8NZ_002251"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="ACG8NZ_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2820..3539)
                     /locus_tag="ACG8NZ_002252"
                     /pseudo
     CDS             complement(2820..3539)
                     /locus_tag="ACG8NZ_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3564..3749)
                     /locus_tag="ACG8NZ_002253"
     CDS             complement(3564..3749)
                     /locus_tag="ACG8NZ_002253"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0539903.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="ACG8NZ_002254"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="ACG8NZ_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="ACG8NZ_002255"
     CDS             complement(5356..6387)
                     /locus_tag="ACG8NZ_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0539904.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="ACG8NZ_002256"
     CDS             complement(6394..7224)
                     /locus_tag="ACG8NZ_002256"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539905.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="ACG8NZ_002257"
     CDS             complement(7221..8036)
                     /locus_tag="ACG8NZ_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539906.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="ACG8NZ_002258"
     CDS             complement(8033..8857)
                     /locus_tag="ACG8NZ_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0539907.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="ACG8NZ_002259"
     CDS             complement(8847..9947)
                     /locus_tag="ACG8NZ_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0539908.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="ACG8NZ_002260"
     CDS             10125..10910
                     /locus_tag="ACG8NZ_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0539909.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="ACG8NZ_002261"
     CDS             10943..11326
                     /locus_tag="ACG8NZ_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0539910.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="ACG8NZ_002262"
     CDS             11402..11533
                     /locus_tag="ACG8NZ_002262"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0539911.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="ACG8NZ_002263"
     CDS             11502..11738
                     /locus_tag="ACG8NZ_002263"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0539912.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11750..11836
                     /locus_tag="ACG8NZ_002264"
                     /pseudo
     CDS             11750..11836
                     /locus_tag="ACG8NZ_002264"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11816..12787)
                     /locus_tag="ACG8NZ_002265"
     CDS             complement(11816..12787)
                     /locus_tag="ACG8NZ_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0539913.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13439..>13724
                     /locus_tag="ACG8NZ_002266"
     CDS             13439..>13724
                     /locus_tag="ACG8NZ_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0539914.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         4163 a         2983 c         2379 g         4199 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tact
//
LOCUS       DAZKPO010000077        13301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000077 DAZKPO010000000
VERSION     DAZKPO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.079"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(327..399)
                     /locus_tag="ACG8NZ_002267"
     tRNA            complement(327..399)
                     /locus_tag="ACG8NZ_002267"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Lys,seq:ctt)
     gene            780..1349
                     /locus_tag="ACG8NZ_002268"
     CDS             780..1349
                     /locus_tag="ACG8NZ_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0539915.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1336..2196
                     /locus_tag="ACG8NZ_002269"
     CDS             1336..2196
                     /locus_tag="ACG8NZ_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0539916.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2386..3090
                     /gene="yycF"
                     /locus_tag="ACG8NZ_002270"
     CDS             2386..3090
                     /gene="yycF"
                     /locus_tag="ACG8NZ_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0539917.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3095..4930
                     /gene="walK"
                     /locus_tag="ACG8NZ_002271"
     CDS             3095..4930
                     /gene="walK"
                     /locus_tag="ACG8NZ_002271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0539918.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4927..6243
                     /gene="yycH"
                     /locus_tag="ACG8NZ_002272"
     CDS             4927..6243
                     /gene="yycH"
                     /locus_tag="ACG8NZ_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0539919.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6244..7113
                     /locus_tag="ACG8NZ_002273"
     CDS             6244..7113
                     /locus_tag="ACG8NZ_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0539920.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7168..7977
                     /locus_tag="ACG8NZ_002274"
     CDS             7168..7977
                     /locus_tag="ACG8NZ_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0539921.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8080..8709)
                     /locus_tag="ACG8NZ_002275"
     CDS             complement(8080..8709)
                     /locus_tag="ACG8NZ_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0539922.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8879..10171
                     /locus_tag="ACG8NZ_002276"
     CDS             8879..10171
                     /locus_tag="ACG8NZ_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0539923.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10247..10909)
                     /locus_tag="ACG8NZ_002277"
     CDS             complement(10247..10909)
                     /locus_tag="ACG8NZ_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0539924.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11134..11418
                     /gene="groES"
                     /locus_tag="ACG8NZ_002278"
     CDS             11134..11418
                     /gene="groES"
                     /locus_tag="ACG8NZ_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0539925.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11469..13094
                     /gene="groL"
                     /locus_tag="ACG8NZ_002279"
     CDS             11469..13094
                     /gene="groL"
                     /locus_tag="ACG8NZ_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0539926.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4424 a         2290 c         2858 g         3729 t
ORIGIN      
        1 aaaaggggaa agcagcaagt aaatttagtt tttctattaa atattcttca ctttttaacc
       61 ttttatagta cttagattta atcctatatg tttagagagt tcattggcag catgattttg
      121 caaagcttct cgaattattg ttatattaga aactttactg ttcataatta tgcttctttt
      181 ttaactcaaa gtccaaaggg ctacagtgga ctttgagtta aaaataaaaa gccctagtta
      241 ttttgaaaaa taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt
      301 acctgttccg tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg
      361 attaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat
      421 agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta
      481 cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac
      541 caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt
      601 tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc
      661 ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga
      721 ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga
      781 tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc
      841 ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg
      901 atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg
      961 taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag
     1021 aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa
     1081 tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat
     1141 tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc
     1201 atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt
     1261 tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa
     1321 aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat
     1381 gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat
     1441 aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg
     1501 ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag
     1561 caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc
     1621 gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta
     1681 ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg
     1741 ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga
     1801 acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt
     1861 ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa
     1921 gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg
     1981 gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat
     2041 ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa
     2101 gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga
     2161 ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt
     2221 gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca
     2281 ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg
     2341 gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta
     2401 gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc
     2461 tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa
     2521 cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt
     2581 gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt
     2641 gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac
     2701 cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa
     2761 ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct
     2821 tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc
     2881 ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt
     2941 tggggttatg attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt
     3001 gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt
     3061 tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg
     3121 atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat
     3181 tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga
     3241 tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga
     3301 taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac
     3361 tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga
     3421 tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac
     3481 gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc
     3541 gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga
     3601 acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc
     3661 ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg
     3721 tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt
     3781 gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat
     3841 tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca
     3901 tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat
     3961 ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact
     4021 tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt
     4081 acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc
     4141 tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac
     4201 agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact
     4261 tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa
     4321 agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat
     4381 tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca
     4441 attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat
     4501 ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg
     4561 ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat
     4621 caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt
     4681 gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt
     4741 cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg
     4801 attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa
     4861 ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg
     4921 gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta
     4981 agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta
     5041 gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt
     5101 cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat
     5161 ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta
     5221 gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg
     5281 gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat
     5341 gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa
     5401 ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt
     5461 tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa
     5521 atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg
     5581 aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc
     5641 cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg
     5701 aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca
     5761 gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc
     5821 agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata
     5881 ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt
     5941 ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa
     6001 gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag
     6061 ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg
     6121 caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat
     6181 aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat
     6241 taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg
     6301 tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat
     6361 cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt
     6421 tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca
     6481 ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac
     6541 ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca
     6601 gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt
     6661 aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa
     6721 gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct
     6781 ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat
     6841 atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg
     6901 tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc
     6961 tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa
     7021 acaagcgata aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca
     7081 aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta
     7141 caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt
     7201 atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt
     7261 ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg
     7321 acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc
     7381 gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa
     7441 gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg
     7501 aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg
     7561 gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc
     7621 ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa
     7681 agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt
     7741 attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg
     7801 atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa
     7861 gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat
     7921 gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca
     7981 gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt
     8041 ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat
     8101 gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc
     8161 catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata
     8221 agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg
     8281 tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata
     8341 gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa
     8401 taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat
     8461 caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc
     8521 aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat
     8581 aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc
     8641 tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc
     8701 aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc
     8761 gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt
     8821 gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat
     8881 gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc
     8941 cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct
     9001 gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg
     9061 attttctatg tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat
     9121 cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg
     9181 cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac
     9241 tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg
     9301 gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga
     9361 aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct
     9421 tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac
     9481 atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg
     9541 tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt
     9601 ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca
     9661 agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat
     9721 gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa
     9781 atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa
     9841 tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat
     9901 tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc
     9961 tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt
    10021 aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta
    10081 tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa
    10141 tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat
    10201 tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat actagccctt
    10261 tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg
    10321 atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt
    10381 agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc
    10441 cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact
    10501 cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca
    10561 tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta
    10621 acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa
    10681 caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct
    10741 taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga
    10801 tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa
    10861 ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc
    10921 ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata
    10981 atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa
    11041 agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac
    11101 agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc
    11161 attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct
    11221 gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa
    11281 aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac
    11341 gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc
    11401 atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg
    11461 aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg
    11521 gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg
    11581 tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag
    11641 aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat
    11701 caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa
    11761 tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg
    11821 ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg
    11881 attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac
    11941 atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat
    12001 caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact
    12061 tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata
    12121 tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa
    12181 tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc
    12241 caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg
    12301 gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag
    12361 taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg
    12421 cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag
    12481 aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt
    12541 ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta
    12601 aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat
    12661 tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag
    12721 tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca
    12781 agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg
    12841 aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg
    12901 ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac
    12961 gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg
    13021 ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg
    13081 gcggtatgat gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag
    13141 ataaaaagag gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa
    13201 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc
    13261 tgtttatacg tgtttagagc gtgagacaaa agtgttcttt a
//
LOCUS       DAZKPO010000078        13285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000078 DAZKPO010000000
VERSION     DAZKPO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.080"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8NZ_002280"
     CDS             complement(105..629)
                     /locus_tag="ACG8NZ_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0539927.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8NZ_002281"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8NZ_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0539928.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8NZ_002282"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8NZ_002282"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0539929.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8NZ_002283"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8NZ_002283"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0539930.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8NZ_002284"
     CDS             3157..4026
                     /locus_tag="ACG8NZ_002284"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0539931.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8NZ_002285"
     CDS             4052..4567
                     /locus_tag="ACG8NZ_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0539932.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8NZ_002286"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8NZ_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0539933.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8NZ_002287"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8NZ_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539934.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8NZ_002288"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8NZ_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539935.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8NZ_002289"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8NZ_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0539936.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8NZ_002290"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8NZ_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0539937.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="ACG8NZ_002291"
     CDS             complement(7873..9090)
                     /locus_tag="ACG8NZ_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0539938.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="ACG8NZ_002292"
     CDS             complement(9348..10625)
                     /locus_tag="ACG8NZ_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539939.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8NZ_002293"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8NZ_002293"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0539940.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="ACG8NZ_002294"
     CDS             complement(11547..12491)
                     /locus_tag="ACG8NZ_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0539941.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="ACG8NZ_002295"
     CDS             complement(12493..13179)
                     /locus_tag="ACG8NZ_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0539942.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3775 a         2774 c         2397 g         4339 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    13261 agacactatt tttaaatatt tttcg
//
LOCUS       DAZKPO010000079        13212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000079 DAZKPO010000000
VERSION     DAZKPO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.081"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8NZ_002296"
     CDS             147..1016
                     /locus_tag="ACG8NZ_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0539943.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8NZ_002297"
     CDS             1225..2250
                     /locus_tag="ACG8NZ_002297"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0539944.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8NZ_002298"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8NZ_002298"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0539945.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG8NZ_002299"
     CDS             3333..5036
                     /locus_tag="ACG8NZ_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0539946.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8NZ_002300"
     CDS             5038..7071
                     /locus_tag="ACG8NZ_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0539947.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8NZ_002301"
     CDS             7082..7522
                     /locus_tag="ACG8NZ_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0539948.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8NZ_002302"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8NZ_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0539949.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8NZ_002303"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8NZ_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0539950.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG8NZ_002304"
     CDS             9793..11559
                     /locus_tag="ACG8NZ_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0539951.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG8NZ_002305"
     CDS             11574..12011
                     /locus_tag="ACG8NZ_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0539952.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG8NZ_002306"
     CDS             12024..13175
                     /locus_tag="ACG8NZ_002306"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0539953.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZKPO010000080        12929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000080 DAZKPO010000000
VERSION     DAZKPO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.082"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            697..1014
                     /locus_tag="ACG8NZ_002307"
     CDS             697..1014
                     /locus_tag="ACG8NZ_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0539954.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1218..1583)
                     /locus_tag="ACG8NZ_002308"
     CDS             complement(1218..1583)
                     /locus_tag="ACG8NZ_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539955.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1791..2273
                     /locus_tag="ACG8NZ_002309"
     CDS             1791..2273
                     /locus_tag="ACG8NZ_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539956.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2470..3360
                     /locus_tag="ACG8NZ_002310"
     CDS             2470..3360
                     /locus_tag="ACG8NZ_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0539957.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3685..6156
                     /locus_tag="ACG8NZ_002311"
     CDS             3685..6156
                     /locus_tag="ACG8NZ_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0539958.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6259..7995)
                     /locus_tag="ACG8NZ_002312"
     CDS             complement(6259..7995)
                     /locus_tag="ACG8NZ_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0539959.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7992..8696)
                     /locus_tag="ACG8NZ_002313"
     CDS             complement(7992..8696)
                     /locus_tag="ACG8NZ_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0539960.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8827..9330
                     /locus_tag="ACG8NZ_002314"
     CDS             8827..9330
                     /locus_tag="ACG8NZ_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0539961.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9290..9628
                     /locus_tag="ACG8NZ_002315"
     CDS             9290..9628
                     /locus_tag="ACG8NZ_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0539962.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9649..11067
                     /gene="ffh"
                     /locus_tag="ACG8NZ_002316"
     CDS             9649..11067
                     /gene="ffh"
                     /locus_tag="ACG8NZ_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0539963.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11179..11757
                     /locus_tag="ACG8NZ_002317"
     CDS             11179..11757
                     /locus_tag="ACG8NZ_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0539964.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11761..12282
                     /locus_tag="ACG8NZ_002318"
     CDS             11761..12282
                     /locus_tag="ACG8NZ_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0539965.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12361..12915)
                     /locus_tag="ACG8NZ_002319"
     CDS             complement(12361..12915)
                     /locus_tag="ACG8NZ_002319"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0539966.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         4273 a         2267 c         2605 g         3784 t
ORIGIN      
        1 ataatacaaa aaggcaaaga tattttcaaa acattttttc ttgatgtgat atccttgtct
       61 tttttgtaat gtttttaact tattgctttt gcaaaattta tttacctgtt ttctggatca
      121 acgatagtgt atattatgct atccttgttt ggattttagg acgtttaaag aggcactttt
      181 ttacataact taatccatag accttttaag catgctgtga gataataata aagaagagtt
      241 taaagcgcac cccaaaccac ttccccataa gtgtattacg ctttaaactc ttttatattt
      301 gaattcacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg cttataatta
      361 ggatttagag taattaatta gatgtttatt ttataggact gatattatag attacaaata
      421 gtatcgtatt tcgcaatctt aaaattatct tcttactatg aataagataa taaaatttgt
      481 atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca acgaaagtat
      541 actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg ctgctaaaat
      601 atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct aaacataatt
      661 ttttcggtat ttaatattta attaggaggg aatattttgc ttttttatag taattttatc
      721 ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag tagaaatgca
      781 agtataggtt ttagaactaa acgaagtctg tcttctaaaa aaaattgggt atactcacaa
      841 actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact ttattctttg
      901 aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca
      961 attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac
     1021 tagtgatagt tttggtgata gtgatattaa aaatcagaaa agatagaaaa agcaggagat
     1081 agataaaatg ctgatatttc agcatttttt aagagtataa aagagaaaaa aagtaataaa
     1141 tgtatacatg atataagaaa aacatatatg tataaaaaga agaaagtagc ttctctttgt
     1201 acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc taccgcccat
     1261 aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat cagctgggtc
     1321 ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa
     1381 gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa
     1441 taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac ggaatgataa
     1501 cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag caacggaagg
     1561 tttttccagc aagtttttat catctttcat caccacctaa caagtgttat tattttgtta
     1621 gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt ctagattaaa
     1681 tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa tttttttaga
     1741 tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta atgagaagaa
     1801 aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt ttgctacctt
     1861 ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc caaggaagcg
     1921 gatttgtggt ttctaggaat tttccagctt tgactgtttt ctatttagtg ataagtgtag
     1981 gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt tacttttctt
     2041 gcttattagt atttacatct atgttttctt tgctgcctcg cttgattatc ggccctaatg
     2101 gtttgagcga gagtggattg aaggattcag cttattcctc catgtatttc ttgacgatca
     2161 aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt tacttctgga
     2221 cagaaatcag gataaaaagt gaacgatctg tatctataaa aaataactat tgatggtgga
     2281 tcatcagtaa aaaagcatat gagctgcgta aaatgcatgt ttctcatatg ctttttttat
     2341 tattatgtaa agaatgcgaa aaacgtttac atgattttta cataacttaa ctatattttt
     2401 acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa ataagaaagg
     2461 agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct tttagccgga
     2521 tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc aagtgactca
     2581 aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt agtcgatgca
     2641 gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca aggaggagga
     2701 agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa ctctgatgta
     2761 tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa agtagcagtg
     2821 gtcgggatgg cgccaatcgt caataaagat acagatgtca aagatatcac caaacaggaa
     2881 ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg gaaagatcaa
     2941 aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt cgaaaaatgg
     3001 ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg aacagttcgg
     3061 cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata tctcgatgat
     3121 tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt agcagataac
     3181 agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc ccctgaagtc
     3241 caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt caaagaattg
     3301 ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa tatcaaataa
     3361 ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac tatgtttccc
     3421 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     3481 ctaataccga gggtcagctg attaaacacc gtttattcga aaaaaagagc tacgacatag
     3541 aactgtcgta gctctttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg
     3601 tgaggaacac tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa
     3661 aacgaaatca atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag
     3721 aaagtaacac aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga
     3781 ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat
     3841 cccacattca aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg
     3901 atctttctta tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt
     3961 ttaccagtta tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag
     4021 atcttggacg aactaaatgt tttaaataca gcaaaagtca atgtcatacg ggacgggata
     4081 gaacaaacaa tagatataga agaccttgta atagatgaca ttgttatttt agagactgga
     4141 catcaaattc cagcagatgc agaggtcgtg gaaggaaaac tgcaagttaa tgaggcttta
     4201 ttgacaggcg aagcagatga aataaccaaa gaaataggcg atccattaat gtccggcagt
     4261 ttcattgttt ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt
     4321 tcccagctga cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct
     4381 tctcttaatc aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt
     4441 ttttctcaaa gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa
     4501 gcagcattga tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca
     4561 ttaagtgcga ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag
     4621 acattggcaa gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa
     4681 atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa
     4741 ttggaagcat gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa
     4801 gctgttaaag catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt
     4861 tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg
     4921 cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct
     4981 gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta
     5041 ttgacagaag cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa
     5101 gcaagagcca catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga
     5161 gataatccgc tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag
     5221 tacgttgatg catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca
     5281 gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac
     5341 aatacagtag ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac
     5401 tgcagtatag cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta
     5461 cttgattctg acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac
     5521 aatattgagc gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca
     5581 ctgttttcag tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt
     5641 agtttgttta ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga
     5701 atcagaggga aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat
     5761 atgatcgtag tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat
     5821 gtttctacag catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc
     5881 tgttcaccgc tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt
     5941 ttttcggtca tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt
     6001 ttgctgttga gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg
     6061 gtagaatatt tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct
     6121 aaaaaatttc tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta
     6181 aaaaagaaca accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc
     6241 acgagcagtt gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc
     6301 aagatgactg tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc
     6361 taaccctaat ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg
     6421 ctcaaagatc cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa
     6481 cgatagatca tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg
     6541 gacagcattt tcaatcagat ttttgataat tggataaagg atatctgttt tagtagtaaa
     6601 tggttcgttc tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata
     6661 attttgaacg atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt
     6721 ctcgccatct tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc
     6781 tttttgcatg atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag
     6841 taaagtctcg gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt
     6901 gccaacgaaa tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac
     6961 ccctagaatc tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt
     7021 ctgccgacct tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg
     7081 atgaatcagc tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg
     7141 atagcttacc acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc
     7201 agcatcatca atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc
     7261 ttctgtggta gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt
     7321 ctgatacaat tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt
     7381 ttgcaccatt ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat
     7441 cagataatag atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca
     7501 acggcgaaaa ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat
     7561 ggcttctaac tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa
     7621 agtagtactt ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc
     7681 gggacgggag tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc
     7741 agtagcatcc attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt
     7801 tttgttttgc gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt
     7861 ttttgtcagg tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa
     7921 ttgccagcca ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat
     7981 ttttttccat ttcatcgttt tggttcctga aacttatagc caaatcctct aatagtcacc
     8041 aagtactttg gatgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca
     8101 tctacgattc ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct
     8161 cggtcgatca cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt
     8221 gttagttcaa tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt
     8281 tgtccaatcg atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatc
     8341 cgtctgaata ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa
     8401 tagtcgtcag cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc
     8461 agcatcaaaa tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca
     8521 tccatcgttg gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat
     8581 tctaatcctt cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg
     8641 aacgtcaaga gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa
     8701 atctccttta aataggtttc attctgttta tattttagca tagaatcagt acaaagggct
     8761 attttacaaa agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag
     8821 cagtcaatga ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag
     8881 aaaaaagaag taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt
     8941 ccagaagtga tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat
     9001 ggattcgatg tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg
     9061 gaagcgtaca aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat
     9121 ctagtcatca tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt
     9181 ttttccggaa taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa
     9241 atgactgtgc tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga
     9301 aaagaccaat cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca
     9361 aatgaactac atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga
     9421 atacgaagta agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga
     9481 ggattatgaa aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga
     9541 aaatatgact gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt
     9601 acagcaaatt caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa
     9661 agcttaactg aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca
     9721 gaagctgacg taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt
     9781 aacttgcaag tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa
     9841 gtgctggaaa gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg
     9901 atcacacttg gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc
     9961 atgatggctg gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat
    10021 ttaaagaaaa atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct
    10081 gcgatcgacc aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga
    10141 actgaagtca gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa
    10201 aacgactatg tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat
    10261 gagttgaaac aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc
    10321 atgactggtc aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca
    10381 ggggtcgtca tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc
    10441 tcagtgacag gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa
    10501 gtattccacc cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg
    10561 attgaaaaag cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg
    10621 cgggaaaatt cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg
    10681 ggcccactgg aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa
    10741 aacgtcaagg tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg
    10801 ccagcagaaa gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc
    10861 ggttctggaa acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga
    10921 aaaatgatgc agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt
    10981 ggttcaggcg tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat
    11041 aagaagaaaa agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa
    11101 tgtctactgc cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta
    11161 aacgaagagg tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa
    11221 tatcatgata ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg
    11281 atgcttgata ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca
    11341 ttcgatgaag catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa
    11401 atcagtgaac taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc
    11461 gtccaaaacg ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat
    11521 ctttggtcgt tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc
    11581 ccaacaacaa gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa
    11641 tttatcggaa gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac
    11701 cttgacagtt gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc
    11761 atgcgaaaaa tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg
    11821 gatgatcttc aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt
    11881 tatgaaacaa tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat
    11941 ttggagaccg aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa
    12001 ttacaattgc cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta
    12061 caaatgacag aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta
    12121 ttggaacatc aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac
    12181 gggattcctt tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca
    12241 cgattcggtg aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa
    12301 aggcaactat atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt
    12361 ttactctgtt aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact
    12421 taatgttcct tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata
    12481 tgcttccggt tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg
    12541 gtttgcgcca aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg
    12601 atcccaaaca ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc
    12661 tggcacagaa cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc
    12721 ctcacgaaaa cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta
    12781 aaatggcaga tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc
    12841 aaaagcttct gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc
    12901 aatttttttt gtcatacgat ttttcctcc
//
LOCUS       DAZKPO010000081        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000081 DAZKPO010000000
VERSION     DAZKPO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.083"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8NZ_002320"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8NZ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0539967.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8NZ_002321"
     CDS             complement(323..892)
                     /locus_tag="ACG8NZ_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0539968.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8NZ_002322"
     CDS             1078..1980
                     /locus_tag="ACG8NZ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0539969.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8NZ_002323"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8NZ_002323"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0539970.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8NZ_002324"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8NZ_002324"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0539971.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8NZ_002325"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8NZ_002325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0539972.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8NZ_002326"
     CDS             8554..8739
                     /locus_tag="ACG8NZ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0539973.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8NZ_002327"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8NZ_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0539974.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8NZ_002328"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8NZ_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0539975.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8NZ_002329"
     CDS             11896..12705
                     /locus_tag="ACG8NZ_002329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0539976.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKPO010000082        12742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000082 DAZKPO010000000
VERSION     DAZKPO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.084"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..544
                     /locus_tag="ACG8NZ_002330"
     CDS             101..544
                     /locus_tag="ACG8NZ_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0539977.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="ACG8NZ_002331"
     CDS             complement(550..1734)
                     /locus_tag="ACG8NZ_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0539978.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="ACG8NZ_002332"
     CDS             2002..2700
                     /locus_tag="ACG8NZ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0539979.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="ACG8NZ_002333"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="ACG8NZ_002333"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0539980.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="ACG8NZ_002334"
     CDS             complement(3510..4043)
                     /locus_tag="ACG8NZ_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539981.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="ACG8NZ_002335"
     CDS             complement(4340..6721)
                     /locus_tag="ACG8NZ_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0539982.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACG8NZ_002336"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACG8NZ_002336"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0539983.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="ACG8NZ_002337"
     CDS             7422..7961
                     /locus_tag="ACG8NZ_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0539984.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="ACG8NZ_002338"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="ACG8NZ_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0539985.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="ACG8NZ_002339"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8NZ_002339"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="ACG8NZ_002340"
     CDS             9068..10234
                     /locus_tag="ACG8NZ_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0539986.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="ACG8NZ_002341"
     CDS             10280..11776
                     /locus_tag="ACG8NZ_002341"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0539987.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="ACG8NZ_002342"
     CDS             11857..12540
                     /locus_tag="ACG8NZ_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0539988.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12549..>12742
                     /locus_tag="ACG8NZ_002343"
     CDS             12549..>12742
                     /locus_tag="ACG8NZ_002343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539989.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3609 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta tt
//
LOCUS       DAZKPO010000083        12492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000083 DAZKPO010000000
VERSION     DAZKPO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.085"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8NZ_002344"
     CDS             <1..375
                     /locus_tag="ACG8NZ_002344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539990.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8NZ_002345"
     CDS             complement(324..893)
                     /locus_tag="ACG8NZ_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0539991.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8NZ_002346"
     CDS             complement(967..2256)
                     /locus_tag="ACG8NZ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0539992.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG8NZ_002347"
     CDS             2514..3458
                     /locus_tag="ACG8NZ_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0539993.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG8NZ_002348"
     CDS             3472..4545
                     /locus_tag="ACG8NZ_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0539994.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG8NZ_002349"
     CDS             4535..6706
                     /locus_tag="ACG8NZ_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0539995.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8NZ_002350"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8NZ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0539996.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG8NZ_002351"
     CDS             complement(9009..11195)
                     /locus_tag="ACG8NZ_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0539997.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8NZ_002352"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8NZ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0539998.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG8NZ_002353"
     CDS             complement(11417..11857)
                     /locus_tag="ACG8NZ_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0539999.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG8NZ_002354"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG8NZ_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aa
//
LOCUS       DAZKPO010000084        12343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000084 DAZKPO010000000
VERSION     DAZKPO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.086"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8NZ_002355"
     CDS             <1..154
                     /locus_tag="ACG8NZ_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0540000.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8NZ_002356"
     CDS             complement(208..486)
                     /locus_tag="ACG8NZ_002356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540001.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8NZ_002357"
     CDS             complement(601..1218)
                     /locus_tag="ACG8NZ_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540002.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8NZ_002358"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8NZ_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0540003.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8NZ_002359"
     CDS             2780..3196
                     /locus_tag="ACG8NZ_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0540004.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8NZ_002360"
     CDS             3193..3663
                     /locus_tag="ACG8NZ_002360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0540005.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8NZ_002361"
     CDS             3677..4450
                     /locus_tag="ACG8NZ_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0540006.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8NZ_002362"
     CDS             4464..5276
                     /locus_tag="ACG8NZ_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0540007.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8NZ_002363"
     CDS             5330..6295
                     /locus_tag="ACG8NZ_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0540008.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8NZ_002364"
     CDS             6431..9154
                     /locus_tag="ACG8NZ_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0540009.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8NZ_002365"
     CDS             9167..9799
                     /locus_tag="ACG8NZ_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0540010.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8NZ_002366"
     CDS             10007..10231
                     /locus_tag="ACG8NZ_002366"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0540011.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8NZ_002367"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8NZ_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0540012.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8NZ_002368"
     CDS             10556..10825
                     /locus_tag="ACG8NZ_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0540013.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG8NZ_002369"
     CDS             complement(11004..12305)
                     /locus_tag="ACG8NZ_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0540014.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4032 a         1999 c         2422 g         3890 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattca att
//
LOCUS       DAZKPO010000085        12200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000085 DAZKPO010000000
VERSION     DAZKPO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.087"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG8NZ_002370"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG8NZ_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0540015.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG8NZ_002371"
     CDS             complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG8NZ_002371"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0540016.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG8NZ_002372"
     CDS             complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG8NZ_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0540017.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG8NZ_002373"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG8NZ_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0540018.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="ACG8NZ_002374"
     CDS             3632..4111
                     /locus_tag="ACG8NZ_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0540019.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="ACG8NZ_002375"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="ACG8NZ_002375"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0540020.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="ACG8NZ_002376"
     CDS             5412..5540
                     /locus_tag="ACG8NZ_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0540021.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="ACG8NZ_002377"
     CDS             5544..6089
                     /locus_tag="ACG8NZ_002377"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0540022.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG8NZ_002378"
     CDS             complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG8NZ_002378"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0540023.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8NZ_002379"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8NZ_002379"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0540024.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8NZ_002380"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8NZ_002380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0540025.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8NZ_002381"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8NZ_002381"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0540026.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3342 a         2529 c         2202 g         4127 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc
//
LOCUS       DAZKPO010000086        11813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000086 DAZKPO010000000
VERSION     DAZKPO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.088"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG8NZ_002382"
     CDS             complement(<1..205)
                     /locus_tag="ACG8NZ_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540027.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACG8NZ_002383"
     CDS             complement(221..835)
                     /locus_tag="ACG8NZ_002383"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0540028.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACG8NZ_002384"
     CDS             complement(982..1665)
                     /locus_tag="ACG8NZ_002384"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0540029.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACG8NZ_002385"
     CDS             complement(1774..2232)
                     /locus_tag="ACG8NZ_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0540030.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACG8NZ_002386"
     CDS             complement(2316..4079)
                     /locus_tag="ACG8NZ_002386"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0540031.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACG8NZ_002387"
     CDS             complement(4300..5760)
                     /locus_tag="ACG8NZ_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540032.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8NZ_002388"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8NZ_002388"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0540033.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACG8NZ_002389"
     CDS             complement(6917..8344)
                     /locus_tag="ACG8NZ_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0540034.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACG8NZ_002390"
     CDS             complement(8375..9589)
                     /locus_tag="ACG8NZ_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0540035.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9675..9902)
                     /locus_tag="ACG8NZ_002391"
     CDS             complement(9675..9902)
                     /locus_tag="ACG8NZ_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540036.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9930..10172)
                     /locus_tag="ACG8NZ_002392"
     CDS             complement(9930..10172)
                     /locus_tag="ACG8NZ_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0540037.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10204..10761)
                     /locus_tag="ACG8NZ_002393"
     CDS             complement(10204..10761)
                     /locus_tag="ACG8NZ_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0540038.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10774..10962)
                     /locus_tag="ACG8NZ_002394"
     CDS             complement(10774..10962)
                     /locus_tag="ACG8NZ_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0540039.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="ACG8NZ_002395"
     CDS             complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="ACG8NZ_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0540040.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3465 a         2447 c         1934 g         3967 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     9841 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc
     9901 atttcaacat aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact
     9961 aatactaaaa tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt
    10021 ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt
    10081 gcgatgatcc ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata
    10141 ccgccgacga ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac
    10201 ttcttattgt acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc
    10261 agattttgct ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt
    10321 tactttgtct tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc
    10381 aacattcaca ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat
    10441 caattctttg atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc
    10501 tagatccaca gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc
    10561 tgtgttgact aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc
    10621 atctacatct ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa
    10681 ttcaccattc atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt
    10741 attcatgttt ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat
    10801 tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa
    10861 caatgtcttg aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc
    10921 aaagataata gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc
    10981 cactcgagag tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac
    11041 tgccactttg accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg
    11101 catcaataca cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg
    11161 gatatggatt cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc
    11221 ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc
    11281 atcttcccgt ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa
    11341 gatgataata gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta
    11401 gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc
    11461 attgatcaac gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat
    11521 tatttttaac cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa
    11581 ctctaccgac aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg
    11641 ctaccaaaca ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa
    11701 ttgcttactt attctctatt tacttttgat tgctggctat acttaaagga tattattcaa
    11761 gattaaaaat tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DAZKPO010000087        11328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000087 DAZKPO010000000
VERSION     DAZKPO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.089"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8NZ_002396"
     tRNA            12..84
                     /locus_tag="ACG8NZ_002396"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8NZ_002397"
     tRNA            88..160
                     /locus_tag="ACG8NZ_002397"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8NZ_002398"
     tRNA            168..249
                     /locus_tag="ACG8NZ_002398"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8NZ_002399"
     tRNA            267..339
                     /locus_tag="ACG8NZ_002399"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8NZ_002400"
     tRNA            351..422
                     /locus_tag="ACG8NZ_002400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8NZ_002401"
     tRNA            439..524
                     /locus_tag="ACG8NZ_002401"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8NZ_002402"
     tRNA            535..608
                     /locus_tag="ACG8NZ_002402"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8NZ_002403"
     tRNA            618..691
                     /locus_tag="ACG8NZ_002403"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8NZ_002404"
     tRNA            711..783
                     /locus_tag="ACG8NZ_002404"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8NZ_002405"
     tRNA            811..884
                     /locus_tag="ACG8NZ_002405"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8NZ_002406"
     tRNA            898..971
                     /locus_tag="ACG8NZ_002406"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8NZ_002407"
     tRNA            995..1084
                     /locus_tag="ACG8NZ_002407"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8NZ_002408"
     tRNA            1097..1170
                     /locus_tag="ACG8NZ_002408"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8NZ_002409"
     tRNA            1189..1264
                     /locus_tag="ACG8NZ_002409"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8NZ_002410"
     tRNA            1266..1336
                     /locus_tag="ACG8NZ_002410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8NZ_002411"
     tRNA            1350..1423
                     /locus_tag="ACG8NZ_002411"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8NZ_002412"
     tRNA            1448..1535
                     /locus_tag="ACG8NZ_002412"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8NZ_002413"
     tRNA            1557..1628
                     /locus_tag="ACG8NZ_002413"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8NZ_002414"
     tRNA            1651..1740
                     /locus_tag="ACG8NZ_002414"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8NZ_002415"
     tRNA            1753..1826
                     /locus_tag="ACG8NZ_002415"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8NZ_002416"
     tRNA            1845..1917
                     /locus_tag="ACG8NZ_002416"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8NZ_002417"
     tRNA            1921..1993
                     /locus_tag="ACG8NZ_002417"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8NZ_002418"
     tRNA            1998..2068
                     /locus_tag="ACG8NZ_002418"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8NZ_002419"
     tRNA            2081..2154
                     /locus_tag="ACG8NZ_002419"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8NZ_002420"
     tRNA            2166..2237
                     /locus_tag="ACG8NZ_002420"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8NZ_002421"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8NZ_002421"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0540041.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8NZ_002422"
     CDS             3826..5298
                     /locus_tag="ACG8NZ_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0540042.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8NZ_002423"
     CDS             5381..6169
                     /locus_tag="ACG8NZ_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0540043.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8NZ_002424"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8NZ_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0540044.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8NZ_002425"
     CDS             7019..8731
                     /locus_tag="ACG8NZ_002425"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0540045.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG8NZ_002426"
     CDS             8734..10506
                     /locus_tag="ACG8NZ_002426"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0540046.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11328)
                     /locus_tag="ACG8NZ_002427"
     CDS             complement(10588..>11328)
                     /locus_tag="ACG8NZ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0540047.1"
                     /translation="YNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEI
                     KKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNK
                     ITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISK
                     NGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHED
                     SKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3264 a         2025 c         2557 g         3482 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa
    11281 tttaaaaatc attcctgtaa aagtttttaa atgtattaca taattata
//
LOCUS       DAZKPO010000088        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000088 DAZKPO010000000
VERSION     DAZKPO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.090"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8NZ_002428"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8NZ_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0540048.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8NZ_002429"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8NZ_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0540049.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG8NZ_002430"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG8NZ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0540050.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8NZ_002431"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8NZ_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0540051.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8NZ_002432"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8NZ_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0540052.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG8NZ_002433"
     CDS             complement(6343..7062)
                     /locus_tag="ACG8NZ_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0540053.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG8NZ_002434"
     CDS             complement(7059..8372)
                     /locus_tag="ACG8NZ_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0540054.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG8NZ_002435"
     CDS             complement(8453..8773)
                     /locus_tag="ACG8NZ_002435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0540055.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG8NZ_002436"
     CDS             complement(8881..9168)
                     /locus_tag="ACG8NZ_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540056.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG8NZ_002437"
     CDS             9295..9939
                     /locus_tag="ACG8NZ_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0540057.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG8NZ_002438"
     CDS             complement(9998..10705)
                     /locus_tag="ACG8NZ_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0540058.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG8NZ_002439"
     CDS             complement(10925..11095)
                     /locus_tag="ACG8NZ_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540059.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKPO010000089        11133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000089 DAZKPO010000000
VERSION     DAZKPO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.091"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1354
                     /gene="lysA"
                     /locus_tag="ACG8NZ_002440"
     CDS             95..1354
                     /gene="lysA"
                     /locus_tag="ACG8NZ_002440"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0540060.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1341..2141
                     /gene="dapF"
                     /locus_tag="ACG8NZ_002441"
     CDS             1341..2141
                     /gene="dapF"
                     /locus_tag="ACG8NZ_002441"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0540061.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2144..3025
                     /gene="dapA"
                     /locus_tag="ACG8NZ_002442"
     CDS             2144..3025
                     /gene="dapA"
                     /locus_tag="ACG8NZ_002442"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0540062.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3022..3738
                     /gene="dapB"
                     /locus_tag="ACG8NZ_002443"
     CDS             3022..3738
                     /gene="dapB"
                     /locus_tag="ACG8NZ_002443"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0540063.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3744..4439
                     /gene="dapD"
                     /locus_tag="ACG8NZ_002444"
     CDS             3744..4439
                     /gene="dapD"
                     /locus_tag="ACG8NZ_002444"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0540064.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4455..5456
                     /locus_tag="ACG8NZ_002445"
     CDS             4455..5456
                     /locus_tag="ACG8NZ_002445"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0540065.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5453..6814
                     /locus_tag="ACG8NZ_002446"
     CDS             5453..6814
                     /locus_tag="ACG8NZ_002446"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0540066.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6774..7925
                     /locus_tag="ACG8NZ_002447"
     CDS             6774..7925
                     /locus_tag="ACG8NZ_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0540067.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7900..9069
                     /locus_tag="ACG8NZ_002448"
     CDS             7900..9069
                     /locus_tag="ACG8NZ_002448"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0540068.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9244..10623
                     /locus_tag="ACG8NZ_002449"
     CDS             9244..10623
                     /locus_tag="ACG8NZ_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0540069.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10904..11133
                     /locus_tag="ACG8NZ_002450"
                     /pseudo
     CDS             10904..11133
                     /locus_tag="ACG8NZ_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3706 a         1945 c         2494 g         2988 t
ORIGIN      
        1 aaaattttgt ttaatattat tccaataaaa ttcaaatctt tatcaaatga aattccaata
       61 aaaacggaaa aaaagtgaaa agaggaatga aaaaatgaca gaattgataa ttggcggagt
      121 cacagcaagc acattggcag aaaagtataa gacacctctg tatgtgtatg atgaagaaaa
      181 gatgagacaa acgatgctgt cattcaagcg aggctttgta tcgaaacgtt ttgatacaaa
      241 agtgctttac gcctctaaag cattccaaac gattgagatg ctgcatttag ttcgtgaata
      301 tggattcggg ctagatgttg tcagtggtgg ggaaatctat accgctttgc aagctgattt
      361 tcctagagat actatctact tccatggtaa caacaagacg ccagaggaac tgatctatgc
      421 acttgaaaat gatttgttgc atattgttgc cgataatttg atggaagttg aattattagc
      481 agaattaagc caacgctatt taaaaaaaat ctgtatcatg cttcggttga atgtcggagt
      541 agaagcacat actcatgaat atatcgtcac tgcgcatatt gattcaaaat tcggcatgag
      601 ctatgaaagt aaagagtgcc aacaagccct tcagttgata aaagaaagca gctatctaga
      661 gttagaagga tttcatgctc atatcgggtc acaaattttt gatgttactg cttggttagc
      721 tgaaatcgac aaattgacga attacttaac cgattttgac caacctcttt ccttgaatct
      781 tggcggaggg tttgggattc gttatacaca aaatgatcag ccgatcccag tcgaagaggc
      841 actaaaaaaa ctgattgaat atacggaaga agctttggaa aaacgaaaac tgaccattcg
      901 tcaattactg atcgaaccgg gtagaagtat tgttggagaa gctggtacaa cactatacac
      961 gattggtttc atcaaaaaga caccacataa aaaatattac tttgtagatg gcgggatgac
     1021 agataatatc cgtccagctc tgtatcaggc agaatacgat tgtgatttag cagcaaaact
     1081 gtcggaagaa aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat caggcgatgt
     1141 ggtaattaaa gaagcttatt tgccgaaagc agaatcgggt gatcttttag ctgtttattc
     1201 cacgggagct tatggatact cgatgagcag caactacaat cgagcgacac gaccagcagt
     1261 tgtttttgtc aataatggcc aatcaagact gattgttcgc agacaagcat tttctgatct
     1321 actaaggggg gaagtttcct atgatccttc ataaatataa tggctgcgga aatgatttta
     1381 tcttattaga ttacgcagaa gacacagatt atagtcaatt agccgcttct ttatgcacaa
     1441 aagaaaaata tgatatagac ggattgatcg ccgtaaaaat cgatccgttg gaaatgatct
     1501 attacaacaa agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc tgttttgctc
     1561 gatacgtaaa tgatcagaac atgatgaatg aacaggaatt caacgtagag acattagcag
     1621 gcacaagaaa aattacggtg ttggatccct caccttttta ttgcagcgta gatatgggag
     1681 tgccagatga ttctccggaa aaaatcaaag ctaatcaaac ttatccaata aaaaaccagg
     1741 aaatagagat caatggtgaa aaagtaacga tcactagttt atttttagga acgattcata
     1801 ccgttgtttt tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt gaactgatct
     1861 gtcatcatcc tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta gtcaatgaac
     1921 aagaattgat cgtccgtacc tatgaaagag gagtaggttg gacacttgct tgcggtacag
     1981 gttgctgtgc ctcttatgtg gttgctaagg aaactgggaa aatttcagca gatcaggtag
     2041 ttgttcactt ggaacaagga gacttgatga tctctggtga ggatgcgata caaatggctg
     2101 gcccagcagt tcatgaatgg acaagaatat tggaggaatg aagatgataa aaggttcgat
     2161 cgtggcgctg atcacgccga tgaatgaaga gggatcagta gattatgctg gtttggaaaa
     2221 attgatccaa ttccatttag atgaacaaac agatggatta ttagttttag gtacaacagg
     2281 agaatcttct acgctcacac aatctgagga ggagcagatc cttcaactga cagtaaagaa
     2341 agtggctggc cgtgtgccgg tgattgcagg agctggaacg aataatacga aagaaacgat
     2401 tgaaaaagcg aagcattttg cttctttggg ggcagacgca cttttggtca tcacccctta
     2461 ctacaacaaa acaagtgatg caggactagc tgctcatttt acagcaattg cagaagcctc
     2521 accgattccg cttattttgt ataatgttcc ttctagaaca ggcatgtcga ttccaatcca
     2581 tgtattagta aatttagcag aacatcctaa tatcattggt ttgaaagaag cctcaggaga
     2641 tatggcttat gtgatggatg ccgctcggtt gattggagaa gaattcttcc tttacagtgg
     2701 aaatgatgat ttgatcctac cagtaatgag tgtaggagga agcggtgtaa taagtgtatg
     2761 ggcgaatatc caacctaaaa tagtccatga attagtaaaa gatacgcagg acggaagatg
     2821 gcagcaagca aaagaaaagc aattaaatgc acttgaactg atccatgcct tgttcagtga
     2881 gaccaatcct attcctgtca aagcagccat gtcattatta gacttacctt ccggacctct
     2941 acgcctgcct ttagtttctc tttcaaaaga gaaaaagaaa caattagctc agcttttgtt
     3001 aaaagaaagg acggttgaaa aatgaaaatc ggactgattg gcagtggaca gatgggaaat
     3061 agagtcgaag aagttgcaca agaaagagga gatacggtcc ttcttttacg gacggcacct
     3121 aaagaagcaa ctacgcttgc ttttacggaa ctgccagaac tgcttattga tttttctcat
     3181 ccagataatt tagagaaaat cctttcgtat agcttatcat atcaacttcc gttagtcatt
     3241 ggcacgacag gttatacaca atcacagttc caagaaataa aagaacatgc caaacatgta
     3301 cctgtgatgt atagtgctaa tttttcgttc ggcatcatgg tgatgaatca actaatcaag
     3361 caagcagctg caatgttgca aggctggcag atcgaactga ttgaaaaaca tcatagtcgt
     3421 aaaaaagatg caccatctgg tactgcaaat atgctgcttc aaacaatcca agaagtgcaa
     3481 aagctgcagc ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca aattggtgta
     3541 cacagtattc gtgcaggttc tttacctggt gaacatgatg tattgtttgc tgctactgat
     3601 gaaatattta cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc aggcgcagta
     3661 gaagcagcgg actggttgaa agatcagtca ccaggttatt acaaattaga agatttatta
     3721 atggacaaag gggtgtaaaa aacgtggaag ctcaagaaat catccgctac attcaaacgg
     3781 catctaaaaa gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc gaatttaaag
     3841 aaagtaaagt ttttggtgaa ggaagcagct tcgttgtttt tggtgactat gagacgattg
     3901 ctccggtttt agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa actgctgcaa
     3961 gatacagcgc agttcctttg ctagatatta aaaaaattaa tgctcgtatc gaaccaggtg
     4021 ctattatcag agatcaagtg tcaatcggca acaatgccgt gatcatgatg ggagcgatta
     4081 tcaatattgg tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct gtcttaggtg
     4141 gacgagcgac agtgggcaaa aactgccata ttggtgcagg agctgtctta gcaggtgtga
     4201 tcgaaccagc atcggccaaa ccggttatcg tagaagacgg cgtactggta ggtgcgaatg
     4261 cagtgattgt tgaaggtgtc catatcggaa aagatgcagt cgtggcagca ggagcagtgg
     4321 ttttagaaga tgtggcagca gaaactgttg tcggtggaat acctgcacgt gtattaaaaa
     4381 taagtgatga tcagacaaaa gaaaatacag cattgattgc tgcgttacgc gaattataga
     4441 ggagatggag aaaaatgcgc gaaatagata cctgtattgt gggggcaacc ggattagtcg
     4501 gccaaacgat gctcaaagtg ttagaagaat atgattttcc agtaggacga ttgaaattat
     4561 tagcttcaaa acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa cacactgtac
     4621 aggaattgac agaaacttca tttgaaggat atgatttagc gctattttct gcaggtgctt
     4681 ctgtatccaa agagtttgcg ccaatcgcac gaagtcaggg actatttgtc atagataatt
     4741 cctcagcatg gcgggaagat tcggatattc cattaatcgt tccagaggtc aatctggaag
     4801 actatacatt gaatcggctg attgcaaatc caaattgttc aaccattcaa gcggtacttc
     4861 cgcttaaagc tttacaagaa gcctttggta tccagcgagt caattattcg acctatcaag
     4921 cagtttctgg ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa aatgacgagg
     4981 aaccagtttt gtatccttat aatattagcc aaacggtgat tccggaaatc gatcagcctt
     5041 tggaaaatgg gtatacaaaa gaagaacaga aaatgataaa tgagacccgt aagattctgc
     5101 atcagcctgc tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa aatggacatg
     5161 gagtctctgt ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt agagagtgtc
     5221 tcagccagtt tccagggatt caattagaag atgatctagc agcacataaa tatccgacga
     5281 gcattcttgc tagaggaaca gatatggttt atgttgggag gatcagaaaa gacacttctt
     5341 tagagaacgg attgttgttc tatacgactg ccgataacat acgcaaagga gccgctgcga
     5401 atgccgtaca gattgctgca gctttaatgc agaaaatagc ggaggtggca agatgagtaa
     5461 tacgctgaaa gtagcgaaat tcggcggaac atcgatgtct catgaagagc aattcaaaaa
     5521 agtccgtaca attgttcttt cagatcccaa tcgccaagtg atcgttgttt ctgctttagg
     5581 caaacgtacg tcagatgatg acaaagtaac agacttactt tatttgattt atgcttacct
     5641 tcagcatcat gttgattgga cacccctttg gaaaaggatt tgtgatcgtt tcattcaagt
     5701 aagagatgtt cttgccctgc agttacctat tgaagatgaa ctagcacgca tacagaaaca
     5761 actggaagac gaaaagataa ctgaagatta tttagtaagt agaggtgaat atctgactgc
     5821 tcaactgatg gctgagtatc ttggttacca gtttattgac gcaaaagacc tgattttctt
     5881 tgactatgat gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa aagcatttaa
     5941 aagatatccg aaaatggttg ttccagggtt ttacggggcg aaccctgcag gaaaagaaaa
     6001 attattgggc cgtgggggaa gcgacattac cggcgcgata ctggcgaaga tgttgcaagc
     6061 ggaaagttat gagaattgga cggatgtttc aggaatcatg atggctgatc cgcgaatcat
     6121 cgatcatcct aaaatcatcg aggaaatctc ttttagagaa ctgagagaga tggcttatat
     6181 gggggctaac gttcttcatg aagcagctgt tttcccagtt caagaggctg atatccccat
     6241 ccaaataaaa aataccaatg atcctttcgc tcaagggaca aaaatatcga atcacgaaat
     6301 agaaaaggaa aatggactga caggaatagc tggacgcaag gattttcttt ccatcacttt
     6361 attcaaacgt catatgtccg atgaaatcgg gtttatctgg aaagcaatga gcatttttgc
     6421 taaacatgag atatcaatcg aacatattcc ttcagggatc gacaatatcg gagtgatcgt
     6481 ctcagcagaa gcgattgccg ataagttatt tctcatcacg aaagaattaa aagaaacact
     6541 tggcgtggaa gaaatcgtcg tgatcgaaga cttagctttg atctctgtcg ttgggggtcc
     6601 gcataaagaa ttgattggat tatctgggaa agtgttatcg atattgaaca aattagaaat
     6661 tcgcacatcg atcttatcac aaggagcaca ggagttgaat ctgatcatcg gtgtaccgaa
     6721 taatcaatat gaaacagtag tgaagggtat ctatgaaggg atggtgaata cggatgctga
     6781 aagaatatcg aatggcactc catcaaattc ctgaaatcgg gtttcatgaa ttcaagacaa
     6841 aaaaatattt atatgaacaa gtaaaaaaca gcgaggccgt tatccatgaa atcggcgaaa
     6901 caggactact cctttatttt gataatcagc aagaagaaac gattgctttt cgaacagata
     6961 tcgatgcact gccagtcaaa gaagaaacga atctctcttt tgcttctaag catgaaggtt
     7021 atatgcatgc atgtggtcat gacggacata tggcgatgct tttagggttg actgacttta
     7081 taactaaaca cattgaggat cttatttata atgtagtact gatctatcag ccatcagaag
     7141 aaatttccgg tggcgcacaa agcgttattc agtcaggatt gttggataaa tatgaagtac
     7201 aagcaatatt tggctttcat ctatggcctg gactagctaa gggagaagtt ttctctcgac
     7261 caggaccttt gatggcacaa agcagtgaaa cagacattat cattaaaggc cagtctgcac
     7321 atattgcttc tagtgaaaaa ggaatcgata gtctggaggc agctgtacga tttttgaaga
     7381 atgtttatga ttttgaccgg caattaccgg cagagtggaa tcatctaatg aaatttggcc
     7441 aaatccaagg tggaacgatc cgaaatgttt tagctggaga agttattgta tccggaagta
     7501 tccgttctta cagttcagat atgcaaaatt atttgaagag gggattagac catttagcag
     7561 aacagtttca ctctacagct actatttctt ttcgctataa tgacggctat ccagctgtaa
     7621 tcaaccatag aggcttatat gcagcactgt tagattcaaa caaactacat gaattagatg
     7681 ctccagttct tcaagccgaa gattttggca tatatacgca acaatatcca tgtgtatttt
     7741 tctttttagg tgtcggtgat acccctgctt tacatgatcc gacgttcgat tttgatatgg
     7801 atgttttaga aaatggtgta gaatggtaca aaacgattct ttttacagaa aatcttcttg
     7861 taaaaatcga tagcagttca aaagaggagc gagtaaataa tgatgaattt atccaatcga
     7921 tttagtccta ggctttctag aatagaagta tcaaaaatac gacaattcga tcagcaaata
     7981 tcgtcgatac cagatgtgat caaactcacc ctgggagaac cggattttcc aactcctgaa
     8041 catgtcaagc aagcaggaat agcagcgatt gaagaagact tttctcacta taccggaatg
     8101 agaggattag aggaactgcg ggaagcggct tgtatatttc agcaacagcg ttatggatta
     8161 acttatgatc cgcaaacgga agttttgacg acagttggtg caacggaagc gattgcctct
     8221 gcgttgttat ctgttttgga agaaggagac aaggttctta ttccagcgcc tgcttattca
     8281 ggatatcagc cattagtgga actagctggt gctgaattga tcccgataga tacttctgac
     8341 acagggtttg tctgtcagcc ggaacaattt gaacgagcat ttgaacaata tggatcagct
     8401 gtcaaagcag tcattttaaa ctatcctaac aatcctactg ggacgacatt gtctgcaaaa
     8461 caattgaaag caatcgctga ggttttaaaa aaatatccgg tatttgttat aagtgatgaa
     8521 gtatatgctg aattgactta tagtggaacg catatgtcca tagctactta tcttccagaa
     8581 caaacaatcg tgataagcgg gttatccaag tcacatgcga tgactggttg gcgagtgggc
     8641 tttatttttg ctcaaaaagc ccttattgac gaactaatta aagtccatca atatctggtg
     8701 actgccgcta caacgatgtc gcaaaaagct gcggctgaag cgctaataaa tggggtgaaa
     8761 gatagcgaga atatgaaaga gcaatatcaa acacgaagag actatctggt ccagcaattg
     8821 gcgcctttag gatttgaagt gacccagcca aacggtgcat tttacttgtt ttgcaaactc
     8881 ccagctgcca ttcaaactga ctcttggcag ttttgtgtag aattagcgga acaggcaaaa
     8941 gttgcgtgta tcccaggaat tgcatttggc ccagaaggag aaggttatat ccggatttct
     9001 tatgctagca gcatggaaaa attacatgag gcctgcaaac ggatcaaaga ctttttaaca
     9061 gaacagtgat tcatagattt tatcaaaaaa acaatcgtta attttgcggt atattctgca
     9121 aggagacgat tgttttttaa atttagttca aatgaacgcg tttcttttga ttcataacta
     9181 atgtaatgtt aagtttaaat tgtttagaat gattttaaaa gaatattcaa ggaggtttca
     9241 tcaatggaaa aaatcaaagt tgtcattgta ggtgcatctc atggagggca tcaatcgatc
     9301 ttggaactat tgacacgata cggtgaaaat gtagatatca cgctatttga agcaggagac
     9361 tatatttcct tcatgtcatg tggtatggag ctgtatttag aagacaaggt aacagatgta
     9421 aatgatgtac gtaatttcag accagaaaac tttccgcaat ccaatgttca tattttaaat
     9481 aatcacgaag taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca
     9541 gataacgaaa caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca
     9601 aacaatctgc cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat
     9661 tgggcaacca aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta
     9721 ggtgcgggtt acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc
     9781 gtattgcttg atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat
     9841 attttagaac aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa
     9901 gcttttactg gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca
     9961 gatctagtca ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc
    10021 gacttagatg aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat
    10081 gtgtatgcag taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg
    10141 atcgctttag cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa
    10201 gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac
    10261 tatcattttg cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta
    10321 cgagtgtctt attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat
    10381 ccaaaagtgt ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca
    10441 gtattatcta cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac
    10501 aaactgactt tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga
    10561 caatggagca tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa
    10621 taagaaaacg aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag
    10681 aacagccaag taaaaacagc ttcttatgtt tttaacttgg ctggacttat taccgcagat
    10741 gcaagctttt gaacaccgtt tattttcaaa aaagggactg tgacacgatt tgtgtaccga
    10801 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggttaaat
    10861 atacttttga acttaaatta aagatcgttc atgattattt ggatggcaaa ggcgattgga
    10921 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaaattcggg agaagcacaa
    10981 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
    11041 aaagaaagtt cagcgcttga tcaagaagct aggaataaaa gtaacggcat acacgcgtaa
    11101 atcacgtcgc tacaattctt atcgaggaaa agt
//
LOCUS       DAZKPO010000090        10865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000090 DAZKPO010000000
VERSION     DAZKPO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.092"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1557)
                     /locus_tag="ACG8NZ_002451"
     CDS             complement(46..1557)
                     /locus_tag="ACG8NZ_002451"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0540070.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8NZ_002452"
     CDS             complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8NZ_002452"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0540071.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="ACG8NZ_002453"
     CDS             complement(3384..4460)
                     /locus_tag="ACG8NZ_002453"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0540072.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="ACG8NZ_002454"
     CDS             4656..6401
                     /locus_tag="ACG8NZ_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0540073.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="ACG8NZ_002455"
     CDS             complement(6681..7118)
                     /locus_tag="ACG8NZ_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0540074.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="ACG8NZ_002456"
     CDS             complement(7102..8475)
                     /locus_tag="ACG8NZ_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540075.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="ACG8NZ_002457"
     CDS             complement(8797..9285)
                     /locus_tag="ACG8NZ_002457"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0540076.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="ACG8NZ_002458"
     CDS             complement(9307..9909)
                     /locus_tag="ACG8NZ_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0540077.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(9978..>10865)
                     /locus_tag="ACG8NZ_002459"
     CDS             complement(9978..>10865)
                     /locus_tag="ACG8NZ_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0540078.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         3166 a         2269 c         1918 g         3512 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc cttatgaact
     9961 gaaccccaaa agttgaacta tttaatggac tgtttccgat attctactgg agatagtcca
    10021 tttaacttta gttttatcct tttgttatta taccacctaa tatactcatt taatttactt
    10081 tgaaatatct caattgatcg gaatttttct cgataaaaaa actctgattt aagcacacca
    10141 aaaaaatttt ctattacaga attatctaag caatttcctt ttctagacat gctttgaata
    10201 atgttattct cttttaattt tttttgatat tgtggcatct gatactgcca tccttgatct
    10261 gaatgtagaa tcagtgaacc ctcagttccc ttttttttaa ttgcttgttg aagcatttct
    10321 tcaatcagtt tatatgttgg actagttgat atactataac taattatttc tccgttaaat
    10381 aaatcaagta taggagatag atagattttt cttcctttta tcttgaattc agtgacgtct
    10441 gtcacccatt ttttattggg tgtatctact gaaaaatttc gttttaacac attcttggca
    10501 atttttccta ctgttccttt ataagattta tatcgcttta ttcggatttg acaggtaagt
    10561 cccatttggg acattaattt tctaactgtt ttatgattga ttgtatatcc tttcatcttt
    10621 aacgctaaag tgactctacg ataaccataa gagtttcttg attcttttac aatcgctgta
    10681 atttcttgct ttatcttgct atatttatct ggcttatcta atttttttac ccagtaataa
    10741 taagttgact tcgctaattg tgcaattgaa agtaatagat tcaatttaaa ttcttttttg
    10801 agctgaagga ttgttttaac cttgatttct tcttgctcaa atcttgttct tgaatcaagg
    10861 tttct
//
LOCUS       DAZKPO010000091        10655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000091 DAZKPO010000000
VERSION     DAZKPO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.093"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACG8NZ_002460"
     CDS             23..646
                     /locus_tag="ACG8NZ_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540079.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8NZ_002461"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8NZ_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0540080.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACG8NZ_002462"
     CDS             2142..3023
                     /locus_tag="ACG8NZ_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0540081.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACG8NZ_002463"
     CDS             3026..3796
                     /locus_tag="ACG8NZ_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0540082.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8NZ_002464"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8NZ_002464"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0540083.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACG8NZ_002465"
     CDS             5107..6270
                     /locus_tag="ACG8NZ_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0540084.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACG8NZ_002466"
     CDS             6387..6797
                     /locus_tag="ACG8NZ_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0540085.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACG8NZ_002467"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACG8NZ_002467"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0540086.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACG8NZ_002468"
     CDS             9370..9654
                     /locus_tag="ACG8NZ_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540087.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACG8NZ_002469"
     CDS             9814..10050
                     /locus_tag="ACG8NZ_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0540088.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACG8NZ_002470"
     CDS             10047..10445
                     /locus_tag="ACG8NZ_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0540089.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3718 a         1773 c         2254 g         2910 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttag
//
LOCUS       DAZKPO010000092        10519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000092 DAZKPO010000000
VERSION     DAZKPO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.094"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8NZ_002471"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8NZ_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8NZ_002472"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8NZ_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8NZ_002473"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8NZ_002473"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0540090.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8NZ_002474"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8NZ_002474"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0540091.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8NZ_002475"
     CDS             5704..6489
                     /locus_tag="ACG8NZ_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0540092.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8NZ_002476"
     CDS             6502..7353
                     /locus_tag="ACG8NZ_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0540093.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8NZ_002477"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8NZ_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540094.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG8NZ_002478"
     CDS             complement(8394..8762)
                     /locus_tag="ACG8NZ_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0540095.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG8NZ_002479"
     CDS             8912..9394
                     /locus_tag="ACG8NZ_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0540096.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG8NZ_002480"
     CDS             9431..10441
                     /locus_tag="ACG8NZ_002480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0540097.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3577 a         1748 c         2192 g         3002 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaat
//
LOCUS       DAZKPO010000093        10297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000093 DAZKPO010000000
VERSION     DAZKPO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.095"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..256)
                     /gene="acpP"
                     /locus_tag="ACG8NZ_002481"
     CDS             complement(14..256)
                     /gene="acpP"
                     /locus_tag="ACG8NZ_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0540098.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(378..1379)
                     /gene="plsX"
                     /locus_tag="ACG8NZ_002482"
     CDS             complement(378..1379)
                     /gene="plsX"
                     /locus_tag="ACG8NZ_002482"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0540099.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1428..3464)
                     /gene="recG"
                     /locus_tag="ACG8NZ_002483"
     CDS             complement(1428..3464)
                     /gene="recG"
                     /locus_tag="ACG8NZ_002483"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0540100.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3581..5263)
                     /locus_tag="ACG8NZ_002484"
     CDS             complement(3581..5263)
                     /locus_tag="ACG8NZ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0540101.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5315..5677)
                     /locus_tag="ACG8NZ_002485"
     CDS             complement(5315..5677)
                     /locus_tag="ACG8NZ_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0540102.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5947..6135
                     /gene="rpmB"
                     /locus_tag="ACG8NZ_002486"
     CDS             5947..6135
                     /gene="rpmB"
                     /locus_tag="ACG8NZ_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0540103.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6373..9042
                     /locus_tag="ACG8NZ_002487"
     CDS             6373..9042
                     /locus_tag="ACG8NZ_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0540104.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9497..10243)
                     /gene="truA"
                     /locus_tag="ACG8NZ_002488"
     CDS             complement(9497..10243)
                     /gene="truA"
                     /locus_tag="ACG8NZ_002488"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0540105.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2800 a         2224 c         1852 g         3421 t
ORIGIN      
        1 cttttatttg aaattattta agatggctgc tgatataatc tactgcgcca ccgactgttt
       61 caatttgttc agcgtcttca tctgaaattt ccgtaccgaa ttcatcttca agctctaaaa
      121 cgaattccat aatactaatt gaatcagctt ttaaatcatc tttgatgttc aattcatccg
      181 tcactttatc ggtatctatc tcaaaatgat ttgaaataat tttcgctact ttgttaaata
      241 cttcttcgcg agtcaacttt ttcacctcca agaatcatac acttgtcaat tacctacacc
      301 atggctaatt ttactggcat ttttcaggaa tgtccacctt tttttcaatg gaacggatcg
      361 cttcagatat ccaaatacta ttcttcgctt ttttcaaatt gttcgactaa ttgtccaact
      421 acatctgtct caagcattgt gtggatttgg cggatcgtat agcgaacagc atcggggcct
      481 gttgctccat gagttttaat gactggtgct ttcaagccaa acaaaacggc accgccatgt
      541 ttggaataat ccatttctgc tttcaaagaa cgtaagccgt cttttaaaag catagcgccc
      601 atttttcctt tgatcccttc atttagaata gcagatttta atagattcat catattcatt
      661 gctgttcctt caatagattt caaaacagcg ttgcctgtaa atccatctgt gaccactaca
      721 tcagccacac cattgagtag ctcacgagct tctacgtttc caataaaatt caagcttgtt
      781 tcattctgaa ggagttcata ggcttttttc gtcacttcac taccttttgt tgcttctgtt
      841 ccgttgttta agagtgcgac acgaggacgt ccgatacctc ggactttacg tgcataaaaa
      901 gaacctaaaa taccgtattg caacaaatgt tctggtttgt tttctgcgtt agcgccaaga
      961 tccagcatat cgaaaccggc accttctttc cctactactg gcaaggtaga catcaagcca
     1021 gggcgttcga tatttttgat acgtcctacg atgaacaacc ctgctgctag tagcgcgcct
     1081 gtattccctg ctgaaaatac agcatctgct tcaccgtttt tcactgcttg tgcagcaagg
     1141 accattgatg cagtcttttt tcgtcgaatt gcttttactg gctcatcatc actattgatt
     1201 ttttcgtctg tatggataat cgtgatgttt ttttcatttg ttaagtattt cttgatttct
     1261 gcttcttttc cgtaaagtaa gaattctact tctggaaaat cttgctgcgc taaagccacg
     1321 ccttctacaa ttgcttgagg tgcgtgatct ccgcccattg catctacggc aattttcatt
     1381 gtttccactc ctttttccgt catcagtata aagtatagca cagtctgcta atcgaaataa
     1441 cgattatcct gattttctat ttggaggctt tgcgctaaag gctgatactc aggaagcatc
     1501 tgccaatcct ttatttgcca aagggctgca gcctcttctc tggctacctc caaaacattt
     1561 ccatccgtca ccaagtcagc aatcgcaaat tgaggcagac cagattgacg gaagccaaat
     1621 acttcaccag gaccacgtaa ttccagatct ttttcgctta gtacaaaacc attggtggtc
     1681 tctgtcatga ttttcatccg ttccacacct agttcatttt taggattagc aactaagata
     1741 cagtaggaag catcacttcc gcgtccaaca cgtccacgaa gttggtgcag ctgtgccaat
     1801 ccaaatcgat ccgcatccat gatcagcatg acggtcgcgt tcggcacgtt tacccctact
     1861 tcaattacag tagtcgaaac taatatctgc atctggttgt ctttgaaggc ttccatgatt
     1921 acttcttttt cctgattctt cattttacca tgtaacaagc caacttcata ttttggtgca
     1981 aatagttcac gaagtttttc atagatttcc actgcatttt ttacatctaa tgcttctgat
     2041 tcttcaatca gcggacagat gacatacatt tgatgtcctc ttgccagttc ttttttcgtc
     2101 caatccagta cactgtccaa ttgaggtgtt cttacccagc gtgtttcgat cgggatacgt
     2161 ccagctggaa gttcatcaat gatagataca tccatctcac catatgctgt aatagctaat
     2221 gttctaggaa tcggagtcgc tgtcataaat aagacatctg gtcgccagcc tttttcacga
     2281 agtactttcc gctggttcac gccaaaacgg tgctgctcat ccgtaataac tagacctaaa
     2341 ttctgaaatt cgactccatc ttggatcaat gcatgtgtgc cgatcacgac gttaatttcc
     2401 ccttgctcta gtttttcaag gagctcacgt cgttcttttg ctttcgttga ccccgtaatc
     2461 aatgcgatgg ttacttcatt gggatcaaac agctgttgca agctttccat atgttgttgt
     2521 gccaagattt ctgtaggcac catcaacgca ccttggaaac ctgctgtaac ggtagcgtat
     2581 aacgcaatcg cggcaaccac tgtttttccg ctacctacat ctccctgcaa aagtcgctgc
     2641 atatgctgcg gactgcgaag atcccgacag atttcattcg ttacacgttt ttgtgcattt
     2701 gtcagttcaa aaggtagctt ttgagtaaat tgtttcaatt tttggacatc ataaaggatc
     2761 tccaaaccat tcgtttctgc tttttctgct tttttcaatc cttgaagacg catttgaaat
     2821 aaaaagaact cttcaaaaac gacacgtcgt ttggcttgat ggttttcttc gtggtctttt
     2881 ggaaaatgca tacttcttac agcagaagct cggtcaagca gtcgatattt ctccacaagg
     2941 ttgtttggca gattttcttc aatcaatgtg ccgtattctt gaaacccttt tttgattaga
     3001 tcaactaatg tcgtttgacg aattgatttg ttgacgtgat atatcggcga aaagtcatcc
     3061 attgtttgag aacctaaaat cttcatcccg ttcaaggctt tacgctttgc atcccattta
     3121 ccgtatacgg cgatttcctc cgataaaatg actttttctt ttagataagg ttgattaaaa
     3181 aaagaaacgt tgaacacatc atgttcttgc atcatacgaa attgcaatcg gcttttttta
     3241 taaccaaatc gattcatcac aggaggcgag actacgattc ctttcaaagt aaccttttct
     3301 tgatcctgga tctcgtttag atttctttcc tgaatatctt cgtagcgaaa aggataataa
     3361 gtcagtaagt cttcaatcgt ttggatgccc aagctggcaa gactagctgc tcttttttct
     3421 ccgactcctg ccagcatagt gattggatca tctaaagaac gcataaattt ttcctccctc
     3481 ctttaacaaa aaaaggactt ggtcagcgat catacctgat cactaaccga aggcctttta
     3541 tgaatgaata ctgtgttaga aagatattct gacacagaaa ttattcagca gaaaatagat
     3601 aaggatagac aggctgatct ccttggtgga tctcgatttc caaatcagga tgtttttctg
     3661 ttagtttctc agcaaattca tttgcttgtt caactgttcc ttcatcacct gccaagatcg
     3721 ttacgatctc tgtgtcctca ttgatcattt gttctagtgt acttaacgct gttgctgtga
     3781 tatctgggtt agagatcagg atttttccat cgatcatgcc caaaaagtct ccttctttga
     3841 tctctacacc gtcaattgag gtatctctga tcgcgtgagt gacttgaccg ctcacaacgc
     3901 tagacaacat ttctgtcatc gtttctttgt tttcttctaa agaagcttga tcattgaatg
     3961 aaagcatagc tgtcaatcct tgggaaatcg ttcttgaagg aacaactgct actgggatat
     4021 ctgctacttc tgctgcttga tcagctgcca tgaagatgtt cttattgttt ggcaagacaa
     4081 tcactttatc tgcgtttact tcttgaatcg ctttaacaat gtcttccgta cttggattca
     4141 ttgtttgtcc accactgata acataattag cgcctaaact ttcaaacagc tcttggacgc
     4201 ctttaccagc agcgatcgct acgataccat acgggatacg tggtatttct tttttcgcaa
     4261 actcgttcac ttcttcatcg tgttccaaaa tcgtctcatg ctgcaagcgc atgttgtcga
     4321 ctttgatttt gactaatgaa ccgaattttt gtccataatt caagacttct cctgggtttt
     4381 ctgtatgcac gtggactttg atgatctcgt catcattgac cacaagtaaa gaatctccta
     4441 taccatctaa atagttacgg aattcttcgt aatcgaatgt actatcgact gtaggtcctt
     4501 ctccgatttt taccatgatt tctgtacagt aaccaaaatg gatatcttct gtcgccattt
     4561 gaccttggac actgcgatga tgttctgcat tgaccatatc atccatttct gctggagaag
     4621 gttcatatgt ttcatccgct tggaattctc cattcaatgc acttaaaaag ccctcataaa
     4681 caaacagcag tccttgccct ccactgtcta cgacaccgac ttcttttagg actggaagca
     4741 agtctggagt tttagctaaa gcacgctttg ctccttttac aacagctgtc attacttcaa
     4801 cgacatcatc agtacttttt gctttctttt ctccagcttt cgctgcttct ctagctactg
     4861 tcaaaatcgt tccttctact ggtttcatta ctgctttata cgcagtttca actccatgag
     4921 tgaaggctgc agcaagatca gaagcattta atgagacaac atctggaatt tgcttagaaa
     4981 atccacgaaa caattgagac aaaataacgc cggaatttcc gcgcgcaccc atcagcaatc
     5041 cttttgaaag cattgctgct aattctccta ctttttctga ggttgaatct actactgcct
     5101 tagctccact agtcatagat agattcatat ttgttcccgt atcgccatct ggcacaggga
     5161 atacatttaa tgaattgacg tattctgcat ttacttgcag acgattcgca cctgcctgga
     5221 ccatttcctg gaactggctt gcacttattt ctgttacact cacctagata atcctccttc
     5281 agatcgactg ctgagttacc agccagtctc ctgcctagtc tggcagtaca cgaacacctt
     5341 gtacaaagac attaactgaa ttggcagtta cgcctaataa tgcctctaga ttgtatttca
     5401 ctttttcttg gacgttgcgg gaaacttcag agattttcgt accatagctg ataatcgtat
     5461 aaacatctac agcaatacca ttatcttctt ggcggacaac tactcctctt gagtaatttt
     5521 ctttacgcaa aatctcagta atattatctt taatttgatt tttgctggcc attcctacaa
     5581 caccgaacac atctgtcgcg gcgccaccaa caactgttgc aattacatca ttggtaattt
     5641 caatggttcc agctgacgtt tgaattttta cagccatgaa tgagcctcct taacgggtaa
     5701 aaaactccca tttttcttct atcatatttt atcatagcgc aacttgaaat aaaaggcagt
     5761 tacccaaagt actcaaaaac cttgcaaaat ttcaggtata accttgttta atttacaatt
     5821 attcttctca attgtcaatt gtatctatga aaatgtactt gcaaattccc attctatatg
     5881 atatgttagt ctggtatgag ctgaattatt ctattgctcc aaatcaaggc aaaggaggaa
     5941 attaccatgg caaaagtttg ttactttaca ggtcgcaaga caaaaagtgg aaacaaccgt
     6001 tcacacgcta tgaactcaac aaaacgtact gttaaaccta acttacaaaa agttcgcgtt
     6061 atggtcgacg gaaaacctaa aaaagtttgg gtgtcaactc gtgctttgaa atcaggaaaa
     6121 gttgaacgcg tttaattgaa tgatacttgg accgttcaga tagtgaacgg ttttttattt
     6181 aataaaaatt tttaagggtg ggacttttga ttcctaccct ttttttgcgt ttatttttgt
     6241 tatacgaaca aattatgaag ttatttattc taaattttat ttttttgtga aaactagact
     6301 ctttttaagc agagaaaaac aaggatatga taaactgaat acaacttcat agaaatttgg
     6361 tggaaaataa atatgaaaaa aaatttaaag tcattcttta gagaaaacgg acgctacgca
     6421 gcagctagtt tcctgattcc gtttttgatt ttagcacttg tttatctaag tatcggaatc
     6481 tatcctggaa gcagcagaag tattttggca agtgatgcct tttcacagtt ttctaatttt
     6541 catgccagct tccgaaatgt tcttcttgga aagcaaagta ttttttatac atggaatgct
     6601 tctctaggat tgaattattt atctttaatt tcttactatc taggcgggat cttcacgcca
     6661 ctggtgttct tttttccaaa ccagatgatg ccagatgcac tgtatgttct gactttgctt
     6721 aaggtcggct gtgccggatt ggctttttgg tttcttgcac gtacgttcaa gattcctcgc
     6781 tgggggcatg ttgcacttag cgtcagttac gcattgatct catttatcac tgcacattcg
     6841 gagatcatca tgtggctgga tgcatttttt tatcttccat tagtgatttt ggggattcat
     6901 cgtgttatgg atcttaaacg gccgaaactt ttatttatca gttatttact cttgttttta
     6961 tccagttttt atatgggttt tatgattggc gtattttctt tcttatattt tatcgcccgt
     7021 ctattgacaa actggtcaga atataaaaaa acgattgttc cttatggtat cacttcttta
     7081 ctggcaggcg gcgcttcaat gatcattgta ttgccagcag tgctggatct tcggtcaaat
     7141 ggcgaggcac tgagtcagat cactacgttg aaaacagaag cgacttctta tctggatcta
     7201 atcatgaaaa atatgattgg tgtttatgac acgacaaaat acggatcgat tccgttcgtt
     7261 tacgcaggtc tgctaccatt agtcctctgt ctgttttatt ttgccagtcg gaaagtaccg
     7321 ttgaaaaata aatttttgta cggcagtctt ttcgccatac ttatcgcaag tttttatctt
     7381 gttccattga atttgttctg gcatggtatg catgcaccga atatgttttt attccgttat
     7441 gcctatctgt tctctttctt agtgctcctg cttgcaggaa aaggatggga aaagatagaa
     7501 caagacgatc gtgggctgtt gttaggaatc attttggtcg tagctgctct ctttgcggtg
     7561 gcatggggca tcaaacctga aggaagctac acctatgtga cgatgacttc ctttgtattg
     7621 acagtggttt tcttagggat ttatgctttt gctgtgggat tttatcagca taaaaaactt
     7681 tcattcaatc atctgagtat attgttatta ttgatcatgt ctgctgaagc actggtcaat
     7741 acgaacagta tgatccatgg cattcttgat gattggaatt atgcttcccg cagtttatat
     7801 actgatcctt atcctgactt gaaagctctt gtaaatgaaa caaaaaaaga ttccgactcg
     7861 ttttaccgct tggaaaatct ggatccagta tctgcaaacg attcgatcaa ctatggttac
     7921 agtggtatca gtttgttttc ttctataaga aatcgtaatt cttcctctta cctggataaa
     7981 ttaggtttcc gttcaagagg gacaaatttg aatatccgtt ataacaataa tactttgctg
     8041 atggatgggt ttacaggaat caaatataac atttctaaaa acgaaagcac gttcagtaaa
     8101 tatggcttcc gccaagctgg tcaaagcggc gactatacac tgtatcagaa tgtcaatgca
     8161 ttgccactag catttactgc accgttgtcc gttaatgatg ttgagcagcc agtgacagat
     8221 aatttgacca gtcagacaaa cttgatcaat acattgtctg gtctaaatga acagtatttt
     8281 acattctata cacctacttt gaagaatcag ttgaatacga cgatcacaaa gacaacgact
     8341 ggcgtcactt actcagaaac agcagataac cagccaaaag agctcacttg ggaagtccat
     8401 gtgcctgcaa atacacaagc ttacttaagt ttgttcccaa ccaattttgc gcaattagaa
     8461 agttctagtg cgacgatcac tgtcaatggt acatcgcgaa aaacgcaaat caacatttct
     8521 ggtcaatact atgatcttgg ctattatcct caagagacaa cagtgacatt tactgcaagt
     8581 ttttatggga cgaaagaaat aagtttcatg gagcctaaag tcgttggtct agatgttgta
     8641 gcctatcaag ccgcaatgaa tcagatcaaa gaaaaaggtg tcgaaatgga agccactggc
     8701 agaaaagctt ctgggactgt aaataccgct gaagattcca tgttggtgac aactattcct
     8761 tatgacaaag gatggaaagc taaaattgat gggaaaccag tcaaggtcag caatttcaaa
     8821 gacgcattct tgatgataaa agttccagca ggtaaacata ctgtcacttt cagttacttg
     8881 cctgaaggat ttacgatagg tgcaatcctt tttgtcctat gtattgcatt gtttattctt
     8941 tatgtctggt accttgaccg caatcgcccc ttttatggca cgattgatcc agcaatagaa
     9001 gaacaaccac gaaacacaca cagaagaaga cgtagaaaat aaagaaacga tggaacctgt
     9061 tcagactgtc cgcaccaagg gcaatcaaaa caacgttcca tcgttttttg gttacagaaa
     9121 aaataggtaa caaaaagatg attcacttat caacgcctcg tccacaagtc acgatattca
     9181 tcaaagatga gaagctgttt tttgaaattg ctctttgtga ctcagagtta aatgacaggc
     9241 tcacgacctc tttcttaaac ggtgttcaat cagctgacct tcggtattag ctcaacaaac
     9301 ggcaaaatat gaggagctat tttcccgttt gtttctctaa tactcaggtc aaaacgctga
     9361 tttcacgacc tcttccttaa acggtgttcc agaagtagct tcttcgaaaa taagccgaaa
     9421 ttttgcaaaa ttttgagaag taattttcgt aaataccttc ttatttcttg aagctgacca
     9481 cttctatcat aacctcttat tcatatttca cttcatacaa ataaagtcct tcagggtgtg
     9541 ctgtaggtcc tgctgcatta cgatcttttt tcgcaataat atctgggata ctatcttcag
     9601 gcaatcggcc attgccaatt ttcaaaagcg ttcctactaa gatccgtatc atcttataca
     9661 agaagccatt tcccctaaat gtaaacaaca attcgtcttt tgtttcattt acctccatct
     9721 tagcctcgta gatcgtacgt attttatcct ctatcgagct tcctgatgca caaaaagagg
     9781 tgaaatcgtg agtccctaat aaatcaggaa gcgcccgctg aattttttcc aaatcaagtg
     9841 ggtaaggaaa ataacttgca taatggcgtc gaaaaggact gcgtggtttc cctatatcca
     9901 ctcgaaattg atacgtcttt tctttgacca aatatcttgc gtggaagtct tctgaaacaa
     9961 tttctacttg tcttacagca atatcctctg gtgactgggt atctagcgcg aaacgcattc
    10021 gttctaacgg acgctcctct ggataatcaa aatggattac ttgcccgatc gcatgtactc
    10081 cggcatctgt ccttcccgaa ccaaagaccg tgatttcttt tccatttgcc attttttgta
    10141 atgttttttc tacttcttct tgaacagtac gcccatttgg ctgtttttgg aatccattaa
    10201 aatttgttcc gtcgtacgca atgattgctt tgtaacgtac catgtttttc tctcctttgt
    10261 tcctaaaagg ttatctggaa taatgatttt tctataa
//
LOCUS       DAZKPO010000094        10048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000094 DAZKPO010000000
VERSION     DAZKPO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.096"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..733
                     /locus_tag="ACG8NZ_002489"
     CDS             254..733
                     /locus_tag="ACG8NZ_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0540106.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACG8NZ_002490"
     CDS             766..2994
                     /locus_tag="ACG8NZ_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0540107.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACG8NZ_002491"
     CDS             3123..4766
                     /locus_tag="ACG8NZ_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0540108.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACG8NZ_002492"
     CDS             complement(4839..5384)
                     /locus_tag="ACG8NZ_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0540109.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACG8NZ_002493"
     CDS             complement(5407..5892)
                     /locus_tag="ACG8NZ_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0540110.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACG8NZ_002494"
     CDS             complement(5882..6694)
                     /locus_tag="ACG8NZ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0540111.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACG8NZ_002495"
     CDS             complement(6833..8365)
                     /locus_tag="ACG8NZ_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0540112.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACG8NZ_002496"
     CDS             complement(8474..9505)
                     /locus_tag="ACG8NZ_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0540113.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3353 a         1852 c         1928 g         2915 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa tcctttttta
     9961 tataagttgt ttgcttattg atggattgga taaattaaac catcacaacg agtggaaatt
    10021 atatgaaatt tttcataaaa attcatat
//
LOCUS       DAZKPO010000095        10045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000095 DAZKPO010000000
VERSION     DAZKPO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.097"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..367)
                     /locus_tag="ACG8NZ_002497"
     CDS             complement(47..367)
                     /locus_tag="ACG8NZ_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0540114.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(515..2749)
                     /locus_tag="ACG8NZ_002498"
     CDS             complement(515..2749)
                     /locus_tag="ACG8NZ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0540115.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3228..3494
                     /locus_tag="ACG8NZ_002499"
     CDS             3228..3494
                     /locus_tag="ACG8NZ_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0540116.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3494..5221
                     /gene="ptsP"
                     /locus_tag="ACG8NZ_002500"
     CDS             3494..5221
                     /gene="ptsP"
                     /locus_tag="ACG8NZ_002500"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0540117.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5385..6431
                     /locus_tag="ACG8NZ_002501"
     CDS             5385..6431
                     /locus_tag="ACG8NZ_002501"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0540118.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6448..7671
                     /locus_tag="ACG8NZ_002502"
     CDS             6448..7671
                     /locus_tag="ACG8NZ_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0540119.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7760..8647
                     /locus_tag="ACG8NZ_002503"
     CDS             7760..8647
                     /locus_tag="ACG8NZ_002503"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0540120.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8733..8963
                     /locus_tag="ACG8NZ_002504"
     CDS             8733..8963
                     /locus_tag="ACG8NZ_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0540121.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9020..9985)
                     /locus_tag="ACG8NZ_002505"
     CDS             complement(9020..9985)
                     /locus_tag="ACG8NZ_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0540122.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3196 a         1780 c         2031 g         3038 t
ORIGIN      
        1 ctttactttt gtcccacgct ctttaattat tatgttcaaa atttgtttac acggctaatg
       61 tagcgcctaa ttcatgtttt ggaatcgaga tttcaacaag gcctgccaat tcgattactt
      121 tgatttcatc ctcgtggtaa acggcgtctt tgattttatc taagaaataa tttttagcat
      181 cttcgatttc tttcggctgg attttacgtt tgtcattgat cagcagaatt tcattatgtt
      241 gaggggcttc agtgtagaca atagtggtat ttccggctgt gtagaccttg actagatggg
      301 catcagtgtt ttcaagctgg tttaatacaa gacgggaatg gctgtttgtt acatttacca
      361 atttcatggc gctcacctct tttatgtatt tttaattgct tgtttctctt attataattt
      421 cttttttata aaatgaacag tattttgact tttctgaaaa aagaatgaga cggcatttct
      481 caaagaaata ccgtctcacg ctataaaact ggatttattc agccgtttct tcggctgatt
      541 ctttagccaa acgttctggt ttagaagtaa cgatgatttt tccatcttta tccagttttg
      601 ctttcaactc atggatagaa gggtgatcaa gataaaattc agcgatacca tcttcgattt
      661 gttcttggat cgtgcgtcga agaggacgag cacccataga tggatcatag cctaagtcaa
      721 ctaatttttc tttgacattc gtaggaactt cgatgtgaag ttgctgtgct gccagcattt
      781 ggttgacatc gtctagcatt agtgtgacga ttgtcatcaa gttttctttt gataatgcac
      841 tgaattcgat gatcccgtca aaacggttca gaaattctgg tgtaaagtag ttgtttaatt
      901 ggttcaatac agaacgagta acaccttcgc gtgccgcacc aaaaccaacg tttgcttcta
      961 ctttaccagt tccggcatta cttgtcataa tgatgatcgt atctttaaag ctgaccgttc
     1021 ttccttgtgc atctgtcaat cgaccatcat ctaagatttg caagaacata tgcaaaacat
     1081 ctggatgagc tttttctact tcatctaaca agacaagact gtaaggattt ctccgtactt
     1141 tttctgttaa ttgaccagct tcttcgtatc cgacataacc tggaggagaa ccaatcaatt
     1201 tagagacact atgtttttcc atatattcag acatatcgaa acgaatcatt gaatcttgtg
     1261 aaccgaatag ttcgtaggca agttgtttcg caagctctgt tttacctaca ccagttggtc
     1321 caacaaataa gaaggagccg attggacgat tctgtttgtt taatccaaca cggtttcggc
     1381 gaatcgcttt cgctactcga tctacagcgt tatcttgtcc gatgacatga gctttcaagt
     1441 catctgctaa gtttttcagt tgtgtctgtt ctttttcttt taattcaccc actggaatac
     1501 ctgtccgttg ttcaacgatt ttttccatat ctttttcggt aatcacaggt gtttcctctt
     1561 cagtgagctg acgttctttc attttttgaa gtttattgat ctgatcgcga taataagctg
     1621 ctttttcgaa atcttcttct cgagaagcta aaactttttg ttcttctgct tcttgcagtt
     1681 ttttctcgat tgttttagga tcaacgattt ggatcgtcaa gtttttctta gaacctgttt
     1741 catctagcaa atcgatggct ttatccggca agaagcgatc ttggacgtaa cgattggata
     1801 aagttgcggc tgcttcgatg gcctcatcag tatattttac atgatgatag tcttcataac
     1861 gtttttgcaa acctttcaaa atactgatcg tttcttcgac agttggttca tctacccgta
     1921 caggttgcat acgacgttct aaagcagcgt ctttttcgat gatgcggtac tcatttaagg
     1981 tagtagcacc aaccatttgc aattctcctc gagctaaagc tggttttaaa atattaccag
     2041 catccatatt tccatcacca gcagaaccag caccgactat ttcgtgaact tcatctatga
     2101 ataggatcac attttctgct tgacggatct cttcaatcaa tttttgcata cgttcttcaa
     2161 attgtcctcg tataccagta ccttggacta aagatacgac atctaaacga atgacttctt
     2221 tgtccattaa tttttgcggt acatcaccat ctacgatttt ttgtgctaaa ccttcaacaa
     2281 ctgctgtttt accaacacct ggttctccaa tcaagacagg attgttcttg gttcggcgat
     2341 ttaaaatttc aatgactcgt ttgatttcgt catcgcgtcc gatcacagga tcgatatcac
     2401 cttgtcgtgc ttgttctgtg atattgattc cgtattctcc caataaacct tctgtattgc
     2461 cttgaggtgg ctgaccacca ttgaagttgt tgtttccgcc aaattgtgtt ggtggtgttt
     2521 gctcataatt tccttgctgt tgcatttggc gagacatgga acggaaaaga tcgtccaaac
     2581 tgccaaatcc aaatggatca ttttgggcca tattagtcag acctccattt tgttgatttt
     2641 tgattttttg ataacagctc tgacagtaat cgagttgtgt ccgctgacca ttgacagtgg
     2701 catacaagtg aatcgttgcc tcattcttgc cgcagttttg acaaagcatg aatcattcac
     2761 gtcctttcac aaaaatgggt acatccatat tttacatctc gtttttcatg aggtaaaaga
     2821 ataaccccta gttttttaag agatttatga ggtagaaaag gtgaaatctc tattgaccat
     2881 tcctgacctt tgactcttat tatactaaaa ataaaaaggg aagcaagtaa ttcgtttcat
     2941 atgaaaacaa agagggtcaa aggaaagaat gataaacgtc aggagaagag aaagcgacaa
     3001 gaaaaaggaa ataaacacgt ttgttataaa atgtgaagaa aaggatgaaa aagaatccgg
     3061 aataatttga atacttttat ctgaaattga atgaaaactg ttgtatctaa cgaaaaaaaa
     3121 tgttaatctt tacagatgaa agggtggcat ttgttaggat tctctgaatt actaaaaaag
     3181 aaaaccctca cataaaaaca acactcactt aaaggagacc gattaatatg gaaaagaaag
     3241 aatttcacgt agtagcagaa actgggatcc acgctcgtcc agctacacta ttagtacaaa
     3301 cagcaagcaa atttaactct gatgtaaact tagagtataa aggtaaatca gtaaacttga
     3361 aatcaatcat gggcgttatg tctttaggtg taggtcaagg ttctgatgtt actattactg
     3421 cagaaggtgc tgacgaagct gatgcaatgg cagcaatcgt tgaaacaatg aagaaagaag
     3481 gcttatctga ataatggttg aaatgctaaa agggattgcc gctagtgacg gtgtagccgt
     3541 tgcaaaagct tacctgctag ttcaaccgga tttaacattc agtaaggcaa cagtagaaga
     3601 tactgcggct gaagaagctc gtttggatgc tgcattagct aaatctactg aagaattgca
     3661 gcaaattcgt gaaaaagcag cgcaaagctt aggtgaagca gaagcgcaag tatttgatgc
     3721 ccatttgatg gttctttcag atccagaaat ggtaggtcaa atcaagcaaa atattaaaga
     3781 caacagcgta aatgctgaat cagcgctaaa agaagtaacg gatatgtata tcggtatgtt
     3841 cgaagcaatg gaagataatg cttatatgca agagcgtgct gcagatattc gtgacgttgc
     3901 taaacgtatt ttagcgcact tattaggcgt tactttacca aatccatcaa tgatcaacga
     3961 agaagtggtc gttgttgcac atgacttgac tccaagtgat acagcacaat tagatcgcaa
     4021 ttttgtaaaa gcgtttgtaa cagacattgg tggacgtact tcacattctg ctatcatggc
     4081 acgttcttta gaaattccag caatcgttgg gacaaaagaa attactgcta aagtcaaaga
     4141 aggcgatatt ttggctgtca acgggatcga aggcgatgta atcatcgatc ctacagatga
     4201 acaaaaagct gaatttgaaa aagcaggtgc agattacgca gcacaaaaag cagaatggga
     4261 aaaattaaaa aatgcagaaa ctgtcacagc tgacggcaag cattttgaat tagctgcaaa
     4321 catcggaaca ccaaaagatc tagttggtgt ccataataat ggtggagaag cagttggtct
     4381 gtaccgtact gaattcctgt atatggattc tccagacttc ccaacagaag atgaccaata
     4441 tgaagcttac aaagctgtat tagaaggtat ggaaggaaaa cctgtagttg ttcgtacaat
     4501 ggacatcggt ggagataaag aactaccata tctgcaatta cctcatgaaa tgaatccatt
     4561 cttaggatat cgtgcattgc gtatcagttt atctgaacaa ggcgacgaaa tgttccgtac
     4621 acaaatgcgt gcattgcttc gtgcatctgt tcatggtaat ttacgtatca tgttccctat
     4681 ggtagctaca ctcaaagaat tccgcgcagc aaaagcgatc tttgaagaag aaaaacaaaa
     4741 attgataagt gaaggcaagg aagtatctga tacgatccaa gtcggcatca tgatcgagat
     4801 tccagcagca gcagtcttag cagacaagtt tgcaaaagaa gtagatttct tctctgtagg
     4861 tacgaacgat ttgattcaat acacaatggc agcagaccgt atgaacgaac gcgtttctta
     4921 cttgtaccaa ccatataacc catcaatttt acgtttgatc aaaaatgtaa ttgatgcagc
     4981 acacgctgaa ggaaaatggg caggtatgtg tggagaaatg gctggagatc aaacagctgt
     5041 tccattgcta gtaggtatgg gtctagatga gttctcaatg agtgcgacat ctattcttaa
     5101 aacgcgtagt ttaatgaaac gcttggacac agcgaaaatg gctgaacttg ctgatcgtgc
     5161 attgaaagaa tgcgatacga tggaagaagt cgttgaactt gttcatgaat atgtaaaata
     5221 aacaacgtat caaaagctgg tgtggcttgt aaaagagcta cgccagtttt tttgttcttt
     5281 tttaagaaaa cgtttttgtc agtaagaata gacaaaaata gggcttaagt ttgagtgaca
     5341 gtttgtaggc tatgttatac tatataagtg aagggggacg taaggtgaag gtattattat
     5401 attttgaaag tgaaaagatg cttgcaaaat ctggaatcgg gcgtgcgctt gatcatcaaa
     5461 aacgtgctct gacggaagtc ggtatctctt atacactaga tgaaaaagaa gattatgaca
     5521 tcctgcacat caatacatac ggaatcaata gtcataatat ggtcaataaa gccagaagag
     5581 aaggaaaaaa agttgtctat catgcgcatt ctacagagga agatttccga aattccttta
     5641 tcggatcgaa ccagctttct cctatcgtaa aaaaatatct ggtaggattg taccaaaaag
     5701 cggactatct gatcacgcca acaccttatt caaaacagtt gttggaaagc tatggtatcc
     5761 gtgtgccgat tcaagctatt tccaatggga tcgacttaga gaaatatcac cctgatccaa
     5821 tgaaagaaca aaagttcaga gaatatttca aactgtctcc ggatcaaaaa gtaatcattt
     5881 gtgtcggttt gtttttcgaa cgtaaaggaa tcattgattt cgtagaaatt gctaagaaga
     5941 tgcccgaata cacatttatt tggtttggtc atgttcccat gtactcgatt cctcggaaca
     6001 tccggaaaat tgtaaaagaa gatcatcctg aaaatgtcct gtttcctggt tacattcgtg
     6061 gagagatcat agaaggtgcg tattcaggtg cagatctatt ctttttccct tcttatgaag
     6121 agacagaagg gatcgttgtt ttagaagctt tagcaagcaa acaaaacgta cttgttcgag
     6181 atattccagt gtataatggc tggttagaag atagcaagaa ctgctatatg gggaagacaa
     6241 acgaagaatt tatccgactg atccaacaga ttaccaatca agaactaccg agtacgacac
     6301 aagccggtta tcaaacagca aaagagcgaa gtatcaataa aatcggtgaa gaacttaaaa
     6361 atgtatacga aagtgtactg aatgaaaaag tctctagcaa attaaaaaaa gtaaatcagg
     6421 tcaaggacta gtttggaggc gaaaaacatg aaaatcggct tttttaccga tacctacttt
     6481 ccgcaagtca gcggagttgc tacatcgatc aaaacattaa aacaagaatt agaaagacat
     6541 gggcatgaag tctatatttt tacaacgacc gatcctaatg cggataagtt ggaaaaagat
     6601 gtgattcgaa tgccaagtgt accatttatt tctttcaagg atcgaagagt tgttgttaga
     6661 ggcatgtggt atgcttattt ggttgccaaa gaactagaac tagatcttat tcatacccat
     6721 acagaattcg gagcagggat acttgggaag atggtagcta agaagttgaa aatacctctg
     6781 atccatactt accatacgat gtatgaagac taccttcatt atatcggtaa aggaaaagtg
     6841 atcaggcaat cccatgtaaa atacttgtct agattatttg caaatcatac gacaggtgta
     6901 gtttgtccaa gtgaacgtgt tattgataca ttacgcagct atggtgtaat agcaccgctt
     6961 cgcgtgattc ctactggtat cgacattgaa aaattcaaac gttctgatat cactcagcag
     7021 gatatcagca aattaaggga gtcactaggt cttcaggaaa accatttgat gattctttct
     7081 ttaagcagga tttcttatga gaaaaatatc caagccttga ttaatgggtt tcctcaggtc
     7141 atctccgctt atcctaacgc cagattagtt atcgtaggaa aaggacctta tgtagaagag
     7201 ttagaggaat tgatctcaga actgcagtta gaagaatttg tgcagttcac aggagaagtg
     7261 gacaacaatg atgtagcact ttattataaa gcggcagatt attttgtcag tgcttctact
     7321 tcagaaacac aagggcttac ttatacagaa gcgatggcag ccggaacacc ttgtgtcgtg
     7381 gaaggaaatg cttatttgaa ccgattgttc gatcatcctt cattagggaa aacttttgaa
     7441 accgacgaag actttgcacc agcacttatt gattatattt ccagtgaagt tcctaaagat
     7501 ccaaaagttt tagaagctaa actttatgat atttcatcta cacatttcgg cgaagctatg
     7561 atcgaatttt atcaagacat ggttgtttat catgaagaag agatgcgaag aaaagaagaa
     7621 gaagactcga ttgaacgaat caaaataaaa ttaaattcat ttaaaagata aaattcaaac
     7681 attttttatg aaaaaatcct tgtttctagc gtatactagc atagaagcaa ggatttttga
     7741 tttttaggag gtcgaaaaaa tgaaacttgg ttttatcgga acaggcgtca tgggaagtgc
     7801 tgtcgcacgc catctactag aagcaggaca cgaagtagct gtctataatc ggacaaaagc
     7861 aaaagctgat cctttagtaa cagaaggagc tatctgggca gatacaccaa aagcggtagc
     7921 agaacaaagt aatattctct ttacaatggt aggttacccc aaagatgtag aagaaatcta
     7981 ctatggacag tcggggattt tttctgcaga tatctcagga catattttag ttgatttgac
     8041 tacaagtaca ccaagcctgg cagaaaaaat tgcaaaaaca gcaaaagaaa aaggcgcaga
     8101 tgcgttagat gcaccggtat ccggtggtga cttaggagca aagaatggta cactgacgat
     8161 catggtcggt ggagaagaag ccgtttatga tcaagtactg cctttattca aagaatttgg
     8221 tacgacattt acattgcatg gttctgctgg gaaaggtcag catacaaaaa tggctaatca
     8281 gatcatgatt gcaggtacga tgacaggaat gacggaaatg cttgtctatg cacaaaaaaa
     8341 cggacttgat ttaaagaaag tgattgagac gctttcaggt ggaagtgcag ctaactggtc
     8401 tttaagcaat tattctcctc gtattttaaa agaagattac acacctggtt tttttgtcaa
     8461 acattttatc aaagatttaa agattgcatt agaagaagca gaaaaaatgg atcttgtatt
     8521 gccagcaact acccaagcat tgaaactata tgaagagttg gcagataaag gttttgagaa
     8581 tgacggaacc caagctttga tcaaactttg gtggcctgaa ggaaaaatac cagaaaaaaa
     8641 atcataaaaa acgagttttt ttagagaaaa tcaatgcaag taggctatgc ttttgataca
     8701 atataacaga aataagaaaa ggaggacctc cgatgacaca ttcacaatta gtcgcaatca
     8761 taaaacgact ggaggctatg gtcgagtcag cagataatga actgcaagtt cgccgttttg
     8821 aaaaagaagg cgtagagaag tgtacagtaa cttatgataa agcgaccgat acatttgaat
     8881 taactgaaac agatactcat cagcaatacg aatttgataa tatcgatatc gtagcgatgg
     8941 aaatctatga cttaatccaa taaattttag gataattgac atttgaagtt gaagatgcct
     9001 aaaagaagcc gagaagcaac taatttagtt gcttttcagc ttcttttttg tttaacagtt
     9061 caatttcaaa ctgttctttt tctaattttt tgcggtaact gatcaagatt tgtacaggat
     9121 cgatttcttc ttttgacaat tgacgtttca aagataaatg agcttctttc ttttcataat
     9181 agaagtcagg agaaacgtca ccagaggtaa tcattgtttc tgcaagttga ccataacaaa
     9241 cgaggtagtg ctttttctga ctctcatctg ctgctaatgg catgctgaag gtgagcgatg
     9301 gtgttcttaa aaactgcttt tgttcctgga tccgtctaat aaaataagta actaattcga
     9361 tatctttttg aagcataccg gctttttcga aattcaactg atagatgaaa tgttgttgtc
     9421 tttttttcaa gtaagtcagt agctttttat ttcggccttg aaacagccct tggagttcct
     9481 tttttttctg ttcaaaagtc atttcctgta ggtcaggaag ctggactcgc aaagctaaca
     9541 aactaatttc agacaaccaa ggcatttgat aagtttcttc cattatacgc aaaatagacg
     9601 gcattttttt atagagacga aaaggcccgt atgttcgagc cgtaggagta tctgttaata
     9661 caaatccctt attggtaata tgtacataat tataattact gaaataattc atttgtcggt
     9721 tataaaaagg acggtaatgt tggatcaatt gacactcgag caataaagca tctaattccg
     9781 tgtctactac gacgaaatca aaatctgtta tatgatgaat catacgtaat actttttttg
     9841 aatgctgttt attctgatga aaatagctgc ttactctatt tttgagccgt tttgctttac
     9901 caacatagat gaccttacca tctcggtctt tcataagata gacacccggc agcatgggga
     9961 gagttttcat tttttctttt aacataggat ctccttttac gctttgaata aaaagactat
    10021 aacaaatttt gaaataatgg acaag
//
LOCUS       DAZKPO010000096         9890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000096 DAZKPO010000000
VERSION     DAZKPO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.098"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..938
                     /locus_tag="ACG8NZ_002506"
     CDS             <1..938
                     /locus_tag="ACG8NZ_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0540123.1"
                     /translation="DELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLFFKQGKSAL
                     EYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYR
                     MFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTI
                     VGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGK
                     EFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQS
                     VASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1415..1615
                     /locus_tag="ACG8NZ_002507"
                     /pseudo
     CDS             1415..1615
                     /locus_tag="ACG8NZ_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2327..2932)
                     /locus_tag="ACG8NZ_002508"
     CDS             complement(2327..2932)
                     /locus_tag="ACG8NZ_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540124.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3114..3449
                     /locus_tag="ACG8NZ_002509"
                     /pseudo
     CDS             3114..3449
                     /locus_tag="ACG8NZ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3614..3829
                     /locus_tag="ACG8NZ_002510"
                     /pseudo
     CDS             3614..3829
                     /locus_tag="ACG8NZ_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3967..4938
                     /locus_tag="ACG8NZ_002511"
     CDS             3967..4938
                     /locus_tag="ACG8NZ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0540125.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5078..6391
                     /locus_tag="ACG8NZ_002512"
     CDS             5078..6391
                     /locus_tag="ACG8NZ_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0540126.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            6434..7300
                     /locus_tag="ACG8NZ_002513"
     CDS             6434..7300
                     /locus_tag="ACG8NZ_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0540127.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            7297..8145
                     /locus_tag="ACG8NZ_002514"
     CDS             7297..8145
                     /locus_tag="ACG8NZ_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0540128.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            8159..9631
                     /locus_tag="ACG8NZ_002515"
     CDS             8159..9631
                     /locus_tag="ACG8NZ_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0540129.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9648..>9890
                     /locus_tag="ACG8NZ_002516"
     CDS             9648..>9890
                     /locus_tag="ACG8NZ_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0540130.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         3153 a         1674 c         2009 g         3054 t
ORIGIN      
        1 cggatgaact tgtaataata gagtcttatt tcaaaatgaa tcaatctgtt gctaaaactg
       61 cccattgctt gaatcgttca agacaaacga tccataaagt atatctgttc ttcaagcaag
      121 gaaaatcagc cttagaatat tatcaacagt ataagaaaaa caaatcaaac tgtggtagac
      181 gtccgcttgt tttacccgag gaacaatcag aatatattca aagaaaggtt gttcaaggat
      241 ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat tagttgttct gctcgtacca
      301 tttatcgtat gttcaaaaag gggctatttg attcttctga cttaccgatg aaaggcaagc
      361 gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac tttccgccgc tctattcatg
      421 aacgtgaaaa ggattatagc caattctcaa atgagtttgg tcaccttgaa ggtgacacta
      481 tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt tgaacgatta tcaaaagtta
      541 tcatcacatt gaaaccgtgt ggtagacaag cgattgatat tgaaaaaaaa ttaaatcaat
      601 ggtttgaatc tgtaccgaaa aacctattca aatccatcac ttttgattgt ggaaaggaat
      661 tttcaaattg gaaacagatc agtaatgtca atgatattgc catttatttc gctgatccag
      721 gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg attgttacgt agagatggtt
      781 tattgaaatc tatggatttc aattcagtag atgaattttt tattcaatct gtcgcatcta
      841 aacgaaataa tattcctaga aaatcactgg attatcgaac acctttggaa gtatttttga
      901 gttacgtaag tattgatgat ctgtctaact taatttgaca attaagacat ttaaaaagaa
      961 aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat atttcatttc
     1021 taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac tggggtattg
     1081 tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt tataatgctt
     1141 ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag aaaaaaacaa
     1201 gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag aatgaaacac
     1261 attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta agttttttca
     1321 tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc ccgttattga
     1381 agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa tatttgagaa
     1441 acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga tcacctccta
     1501 ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt gccagttaga
     1561 tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga attgatcaga
     1621 aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg ctcagggcaa
     1681 ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc actttagaat
     1741 acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat ttaaacaagt
     1801 tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca aggcaataga
     1861 tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga ataaccttct
     1921 gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta ttttattggt
     1981 tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa cgattagttt
     2041 aactgtggga agctccagac atacgattcg agaagttact tctcgagcag acaggaaggg
     2101 ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc tttttccgga
     2161 aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc ataaagaact
     2221 aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac atggaacata
     2281 gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac aaatcattcg
     2341 ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca atttctgcaa
     2401 ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt ttttgcggat
     2461 ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct tttttggcca
     2521 ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt gcaatataac
     2581 ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt ccagtattaa
     2641 aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc ataaaggtaa
     2701 ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca acaatgacac
     2761 aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg atatctttac
     2821 cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt gccaattgtc
     2881 gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc ataaaaactc
     2941 cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact gttatcatag
     3001 catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt tgaatgaatg
     3061 aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc tcggaacagt
     3121 taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt agaaaaggca
     3181 aactttatca aacttattac gacggtatgg aggaacagct caacgcatta ggattagtaa
     3241 ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa caaattaaaa
     3301 cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta attagtgatc
     3361 acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg aatggaatac
     3421 tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc taccgtgcat
     3481 tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac acaagaatac
     3541 taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt cgtttctatg
     3601 ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca ttgacgactt
     3661 agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg gtcctgttta
     3721 ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg aaataaaatt
     3781 gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg aatacgaaaa
     3841 tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat tcctgctaat
     3901 ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag aggagttgtt
     3961 cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg tgtgggtacg
     4021 gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa agtaatggct
     4081 gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa aacgaatagt
     4141 agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc cgagtttgcc
     4201 tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg taatgcgaac
     4261 ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa agtagacgga
     4321 attataggta ttacctattc agacattgat aaatatgtat catcaaattt accatttgtc
     4381 agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa ttatttaggt
     4441 gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg ctatgttggg
     4501 ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta caatatttgt
     4561 gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag caatttagag
     4621 gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt taccatcaat
     4681 gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga agcacctaag
     4741 gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga ctatttacta
     4801 tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac actcatggcg
     4861 atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt tcacgctgga
     4921 gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag gttgacagaa
     4981 agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt aattgaaaaa
     5041 taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa tgttagcaat
     5101 tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta aggagaaaag
     5161 ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg atgaaacgcc
     5221 tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg agggtgaata
     5281 taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg aggataaagt
     5341 aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg gaccgaatac
     5401 tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga aagatgcgga
     5461 catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca aaatgtatgc
     5521 gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt tcaaagatgc
     5581 tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga cggaatttca
     5641 agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata tgcaaatggc
     5701 gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga atggcggcga
     5761 tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa aatccgttga
     5821 agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca gtcctattga
     5881 aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga caatggcaga
     5941 tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg tttcagatgc
     6001 aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg agaaaacaga
     6061 gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat ctagtcgtat
     6121 tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt taattaaaga
     6181 tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg gacatgcccg
     6241 cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag ttcaagatat
     6301 tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg ctgctgaaat
     6361 gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca atcaagtgtt
     6421 aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc cagctttgct
     6481 gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg cctttactga
     6541 ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt ttaagcgact
     6601 gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg ttgtttggat
     6661 tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca aaaatcgtaa
     6721 aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct tagtcgttat
     6781 ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata cgctatttgc
     6841 aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa tgtatacgct
     6901 tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt tagggggaat
     6961 gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt ctaagtttca
     7021 gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta ttttacttac
     7081 cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc aaggtggacc
     7141 aatgaattct actatgacaa tggtttatta catttaccaa actggattta cagaccgtat
     7201 ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt taattacaat
     7261 tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt tggaccgcta
     7321 atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt cccgatgctg
     7381 tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt agcttccttc
     7441 aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca agaggttatt
     7501 tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt aacctttgcc
     7561 tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt taatttttca
     7621 ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat ggagacggtt
     7681 ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg cttggcagtg
     7741 attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt ctttatcgcg
     7801 attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt taaaattttc
     7861 tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt cttatccttt
     7921 atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag cagcaaattt
     7981 tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat caatcaagtg
     8041 atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt tgcacaaaaa
     8101 tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg aatagtgaat
     8161 gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa cagtcaatca
     8221 agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg atccaaatgg
     8281 ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt acgacagcat
     8341 ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact gggagcattt
     8401 gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt ccggcagtgc
     8461 catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg tggaagggca
     8521 agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg aaaaatatgc
     8581 cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg aagatttccg
     8641 cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta ctaaaactgc
     8701 agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt gggagtttta
     8761 ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc cagacttgtt
     8821 tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc cacaaggcaa
     8881 tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt ggcaaacagg
     8941 caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt atgcacccca
     9001 gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa tgtggcagcg
     9061 taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct tgcctagaga
     9121 gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt atcaagtaat
     9181 tgatccgcag gaaaggattg aatgggcgaa gactgaagag aatcgcttct tactgaagga
     9241 cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg caacgaaact
     9301 agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg atgttgaacg
     9361 ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag ctgaaaagga
     9421 agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa ccatcgaaat
     9481 tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta tgagtacaac
     9541 tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg aagtaacctt
     9601 ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg atttatggtt
     9661 caatcgaagc aggcggcaca aaatttgtgt gtgccattag cgatgaagaa atgacgatca
     9721 aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta gtcattgact
     9781 tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt gggcctatcg
     9841 atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa
//
LOCUS       DAZKPO010000097         9813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000097 DAZKPO010000000
VERSION     DAZKPO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.099"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG8NZ_002517"
     CDS             complement(34..2154)
                     /locus_tag="ACG8NZ_002517"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0540131.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG8NZ_002518"
     CDS             2384..3562
                     /locus_tag="ACG8NZ_002518"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0540132.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8NZ_002519"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8NZ_002519"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0540133.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8NZ_002520"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8NZ_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0540134.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG8NZ_002521"
     CDS             complement(4915..6168)
                     /locus_tag="ACG8NZ_002521"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0540135.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8NZ_002522"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8NZ_002522"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0540136.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG8NZ_002523"
     CDS             7746..9092
                     /locus_tag="ACG8NZ_002523"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0540137.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG8NZ_002524"
     CDS             complement(9400..9786)
                     /locus_tag="ACG8NZ_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0540138.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3129 a         1782 c         2009 g         2893 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta tta
//
LOCUS       DAZKPO010000098         9741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000098 DAZKPO010000000
VERSION     DAZKPO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.100"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG8NZ_002525"
     CDS             283..522
                     /locus_tag="ACG8NZ_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540139.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG8NZ_002526"
     CDS             complement(884..1144)
                     /locus_tag="ACG8NZ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540140.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ACG8NZ_002527"
     CDS             complement(1329..1826)
                     /locus_tag="ACG8NZ_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0540141.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8NZ_002528"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8NZ_002528"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0540142.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8NZ_002529"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8NZ_002529"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0540143.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="ACG8NZ_002530"
     CDS             complement(4548..5210)
                     /locus_tag="ACG8NZ_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0540144.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="ACG8NZ_002531"
     CDS             complement(5220..6035)
                     /locus_tag="ACG8NZ_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0540145.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="ACG8NZ_002532"
     CDS             complement(6297..6740)
                     /locus_tag="ACG8NZ_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0540146.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="ACG8NZ_002533"
     CDS             complement(6874..7212)
                     /locus_tag="ACG8NZ_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0540147.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="ACG8NZ_002534"
     CDS             complement(7200..7577)
                     /locus_tag="ACG8NZ_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0540148.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="ACG8NZ_002535"
     CDS             7801..8994
                     /locus_tag="ACG8NZ_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0540149.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="ACG8NZ_002536"
     CDS             complement(9160..9582)
                     /locus_tag="ACG8NZ_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540150.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2905 a         1940 c         1594 g         3302 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     9721 tcatttaatc gtttattata t
//
LOCUS       DAZKPO010000099         9595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000099 DAZKPO010000000
VERSION     DAZKPO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.101"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..642)
                     /locus_tag="ACG8NZ_002537"
     CDS             complement(<1..642)
                     /locus_tag="ACG8NZ_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0540151.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTK"
     gene            complement(644..1408)
                     /locus_tag="ACG8NZ_002538"
     CDS             complement(644..1408)
                     /locus_tag="ACG8NZ_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0540152.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1416..2093)
                     /locus_tag="ACG8NZ_002539"
     CDS             complement(1416..2093)
                     /locus_tag="ACG8NZ_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0540153.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2130..3515)
                     /locus_tag="ACG8NZ_002540"
     CDS             complement(2130..3515)
                     /locus_tag="ACG8NZ_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0540154.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3725..3809)
                     /gene="ffs"
                     /locus_tag="ACG8NZ_002541"
     ncRNA           complement(3725..3809)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8NZ_002541"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3832..4347)
                     /gene="tadA"
                     /locus_tag="ACG8NZ_002542"
     CDS             complement(3832..4347)
                     /gene="tadA"
                     /locus_tag="ACG8NZ_002542"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0540155.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4454..5311
                     /locus_tag="ACG8NZ_002543"
     CDS             4454..5311
                     /locus_tag="ACG8NZ_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540156.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5392..5727)
                     /locus_tag="ACG8NZ_002544"
     CDS             complement(5392..5727)
                     /locus_tag="ACG8NZ_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540157.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5794..7164)
                     /locus_tag="ACG8NZ_002545"
     CDS             complement(5794..7164)
                     /locus_tag="ACG8NZ_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0540158.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7242..7778)
                     /locus_tag="ACG8NZ_002546"
     CDS             complement(7242..7778)
                     /locus_tag="ACG8NZ_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0540159.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8087..9568
                     /locus_tag="ACG8NZ_002547"
     CDS             8087..9568
                     /locus_tag="ACG8NZ_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540160.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1970 c         1502 g         3215 t
ORIGIN      
        1 ttttgtttgt ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa
       61 taatacaaaa aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat
      121 tggatcagta acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag
      181 gataaaaaag cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat
      241 gccgatttga aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat
      301 aaacaaaata ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat
      361 tttcacttca taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag
      421 gatggttttg aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt
      481 ctcttgatct aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag
      541 tggtcgaaag ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa
      601 caatccagca tattccatcc actgccatct cgaatttttc atattagaag ctccattcat
      661 ccagtgattg tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg
      721 taaaacctga taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg
      781 tttcatagtt atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg
      841 ctttatccat aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg
      901 tctctacaaa agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat
      961 tcttatctgg gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa
     1021 ctactttttc ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt
     1081 cttcccatgt aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat
     1141 agactttttt cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt
     1201 agactgtctc tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt
     1261 ctggtgactt agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt
     1321 cttccacaaa tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta
     1381 aatcaattaa atatccttta tagtccattc atccactact cttccctttt tctgatcgta
     1441 aatgctgttt ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag
     1501 gtttttgctg actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact
     1561 ggtccttttt tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga
     1621 gttgtctttt cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg
     1681 caatattcgt aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga
     1741 ttatccgctt ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata
     1801 taatcatatc gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct
     1861 tcacgataat cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct
     1921 aacagtgtca ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc
     1981 aaagcagctg tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta
     2041 gcaatttctg ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca
     2101 ccacctatct tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa
     2161 tctcctacca cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca
     2221 atcgttggaa aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta
     2281 ttcagatcga ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt
     2341 ccattataga ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag
     2401 ttacgtaaaa agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca
     2461 cccaatgtga catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga
     2521 ttgacccgcc cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc
     2581 gtctttcctc gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag
     2641 atatctagac tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc
     2701 ccacgttcta gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg
     2761 gtagggatgg ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg
     2821 acttcaccga catactgccc gtactttcca gcggctgcta tttggacatc attgattacg
     2881 tgaccttctc tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt
     2941 tcttttgcaa tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc
     3001 acttgtgaac gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc
     3061 cagccattcg gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc
     3121 gtctggtgtt tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta
     3181 tttttatcga ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc
     3241 aactcttctt tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca
     3301 acttcattca tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa
     3361 cgatccacaa aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt
     3421 tctcgtttac gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc
     3481 agatcattcg tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt
     3541 tatcatacca taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct
     3601 gccttgaagc aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg
     3661 aacattcatt tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt
     3721 ttcattataa tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca
     3781 cgcttcacaa gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc
     3841 attcgttgac cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc
     3901 agtcaaaatc atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt
     3961 aaaccgctcg tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata
     4021 cgcgccgaaa taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca
     4081 aggctctaag gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca
     4141 ggcttccttg attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag
     4201 gttatatcct cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc
     4261 atgaagcatt tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc
     4321 tactgcatca agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt
     4381 actttagcat atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca
     4441 agaggtgaac aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa
     4501 aaactctcca gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg
     4561 gatcaaacaa gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt
     4621 aatcaacgat caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt
     4681 tcaagaaaaa agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa
     4741 aaaagaatgg caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta
     4801 tacagacaca gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga
     4861 aatcaacggt atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt
     4921 tggttctttc cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat
     4981 ttttttaaca gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt
     5041 ggctttacat tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat
     5101 agaactgatc aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg
     5161 caatgtcagc tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat
     5221 cgaaaaattt caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg
     5281 ctatctttta ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat
     5341 tttgtcacaa ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat
     5401 tttttcaaca aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc
     5461 accataaaca gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga
     5521 atccaagtca ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa
     5581 tttgtcatag acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat
     5641 tacttttccc gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc
     5701 gattgttact tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat
     5761 agcatagaca aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa
     5821 aaagttcttt actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac
     5881 tagcaaaacc catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg
     5941 ccattccttt tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc
     6001 taatgcatag ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg
     6061 tacgactttt aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt
     6121 gaacactaaa agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag
     6181 tttttcctgc tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg
     6241 catctgattt tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt
     6301 atgaggccat atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc
     6361 tgaactaacg gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag
     6421 ccagctaatg atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg
     6481 tcctttattt tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca
     6541 gtatagcgga gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc
     6601 ttctcctgaa caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac
     6661 aagttctgcg ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt
     6721 ttctgataaa atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc
     6781 cttcatttga gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac
     6841 tttttcaccg ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct
     6901 atattcatac ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc
     6961 attgctgtag ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc
     7021 tgccggtttt agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga
     7081 aaaaaaatcg gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg
     7141 taacgttgtc gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta
     7201 cgaaaaataa aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt
     7261 aagatatttt ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt
     7321 tcaagaaaat cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc
     7381 aattcaagcg acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc
     7441 cgcttaaata gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca
     7501 tggatagaaa aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat
     7561 tcagctaagt agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg
     7621 ctgattgatt tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac
     7681 tctcttgcag taattgactt cattccatat tcttccacgt ataccatact tttgaacagc
     7741 aaatattctt tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat
     7801 ctacccataa ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat
     7861 atttagttat atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa
     7921 aataccctag gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat
     7981 ataaaaaaga aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca
     8041 gtttttgtct aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc
     8101 aagaaaaaaa gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca
     8161 agaattatcc aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat
     8221 aaatgtatca ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg
     8281 agagatccaa accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa
     8341 attatgctac ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc
     8401 acaaactgct gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct
     8461 cgaaaaactg acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc
     8521 tgtaaaaaat tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt
     8581 ttttctttca gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc
     8641 actaaaaatg aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat
     8701 ctacttgctg ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac
     8761 ttcttctgaa atcactgaat tgatggactt attgcagcat acacatgatt attcagaata
     8821 ctttacttct ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt
     8881 taattatttt ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat
     8941 tggtttttta ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac
     9001 ttacttaact caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta
     9061 ttttcttgtg ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc
     9121 tacactttgc tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa
     9181 attaagagaa aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca
     9241 ataccttagt caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat
     9301 ccatttgcag ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca
     9361 gttatttcgc aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat
     9421 tacagatcag catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca
     9481 caatacagtg ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg
     9541 ttatgagaac aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattc
//
LOCUS       DAZKPO010000100         9527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000100 DAZKPO010000000
VERSION     DAZKPO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.102"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACG8NZ_002548"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACG8NZ_002548"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0540161.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACG8NZ_002549"
     CDS             1011..2258
                     /locus_tag="ACG8NZ_002549"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0540162.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACG8NZ_002550"
     CDS             2334..2678
                     /locus_tag="ACG8NZ_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540163.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACG8NZ_002551"
     CDS             complement(2795..3121)
                     /locus_tag="ACG8NZ_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540164.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="ACG8NZ_002552"
     CDS             complement(3736..4938)
                     /locus_tag="ACG8NZ_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0540165.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="ACG8NZ_002553"
     CDS             5055..5570
                     /locus_tag="ACG8NZ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0540166.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="ACG8NZ_002554"
     CDS             complement(5769..6215)
                     /locus_tag="ACG8NZ_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0540167.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG8NZ_002555"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG8NZ_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0540168.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="ACG8NZ_002556"
     CDS             complement(8094..8723)
                     /locus_tag="ACG8NZ_002556"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0540169.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="ACG8NZ_002557"
     CDS             complement(8707..9171)
                     /locus_tag="ACG8NZ_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0540170.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT         2974 a         1967 c         1693 g         2893 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacct
//
LOCUS       DAZKPO010000101         9170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000101 DAZKPO010000000
VERSION     DAZKPO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.103"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8NZ_002558"
     CDS             134..307
                     /locus_tag="ACG8NZ_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540171.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="ACG8NZ_002559"
     CDS             947..1420
                     /locus_tag="ACG8NZ_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0540172.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="ACG8NZ_002560"
     CDS             1429..1656
                     /locus_tag="ACG8NZ_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540173.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2292..2663
                     /locus_tag="ACG8NZ_002561"
     CDS             2292..2663
                     /locus_tag="ACG8NZ_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540174.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2919..3161)
                     /locus_tag="ACG8NZ_002562"
     CDS             complement(2919..3161)
                     /locus_tag="ACG8NZ_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0540175.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3193..4095)
                     /locus_tag="ACG8NZ_002563"
     CDS             complement(3193..4095)
                     /locus_tag="ACG8NZ_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0540176.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4108..4296)
                     /locus_tag="ACG8NZ_002564"
     CDS             complement(4108..4296)
                     /locus_tag="ACG8NZ_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0540177.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4310..4873)
                     /gene="amaP"
                     /locus_tag="ACG8NZ_002565"
     CDS             complement(4310..4873)
                     /gene="amaP"
                     /locus_tag="ACG8NZ_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0540178.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4911..5804)
                     /locus_tag="ACG8NZ_002566"
     CDS             complement(4911..5804)
                     /locus_tag="ACG8NZ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0540179.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5882..6805)
                     /locus_tag="ACG8NZ_002567"
     CDS             complement(5882..6805)
                     /locus_tag="ACG8NZ_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0540180.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6854..7204)
                     /locus_tag="ACG8NZ_002568"
     CDS             complement(6854..7204)
                     /locus_tag="ACG8NZ_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0540181.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7237..8139)
                     /locus_tag="ACG8NZ_002569"
     CDS             complement(7237..8139)
                     /locus_tag="ACG8NZ_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0540182.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8132..8989)
                     /locus_tag="ACG8NZ_002570"
     CDS             complement(8132..8989)
                     /locus_tag="ACG8NZ_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0540183.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2572 a         1736 c         1509 g         3353 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     1801 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     1861 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     1921 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1981 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     2041 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     2101 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     2161 cattattttg ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac
     2221 acgaaaaagc ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta
     2281 ggaggaatag tatgccattt attgtttact tttttctttc attactcgtg atctatattc
     2341 cattaccaac atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg
     2401 ctcttacgaa actttgttta cttctatttg tttgtctaga ttactttacg agctattatt
     2461 ggctaaaaca gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct
     2521 ttgagtttct gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca
     2581 tctgggtttt caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat
     2641 ttagggaggc attgttttat taaagaagct tctctgtatc aataaagaca catttaagga
     2701 aaaactttta aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt
     2761 cttgtcacag cctcttattc cgtataaaca gtgttcaaaa gcttgcatct tcgataatat
     2821 gcccaaccaa atgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg
     2881 aagaacagct ttttcacgac ctctttttaa aagtcttttt acagttttcg agcaaagaat
     2941 gatacgatta gtactaaaat gatggcacca atcaatgatg gcacgattgc cattcctgca
     3001 atacttggtc cccaagaacc taataatgct tgaccaagtg ctgatccaac taaacctgct
     3061 acgacatttg caataatccc catcgaagaa cctttattcg tgattgctcc tgcaattgct
     3121 ccgataattc ctccgacgat aagtgaccaa ataaatccca tgtttcgttc ctcctaatgt
     3181 tttacttcct tattactgaa cccgtggttc tgtattttct tttacttttt gtgcgccgct
     3241 tccagctgct gattttactt tatctgtttg attcgaagca aattcaccgg tttttttcgc
     3301 tgcgtcagtt actttgtctt gcaccgtttc ttgatctttt tcaaattctt cttttgtttt
     3361 gatgtccact acgttagcgt tgacttcgat cacttctaaa tgtgtcatat tttttacttc
     3421 tttgccgatt acttcttgca tttcttcaaa gattttttcg atatctttgc catattccac
     3481 aatgacatcc atatcaactg ctacttgttt tttaccgact tctgtttcga tcccagccgt
     3541 tacattatct gtattcacaa gttttcctgc cacattagag aagaaaccac cgtttgcaga
     3601 taataaaccg tctacttgtt caattgcgat tccgatgatt ttttgcacaa ctttatcttc
     3661 ataattcagc tcaccgcgca ttgaatcttc ctgttcagaa tcatgctttc cttggttatt
     3721 tgtatgatca gaaccaagtt ttccttgtcc gtttgtgtgg tcagaactaa gtttttcttg
     3781 tgctttcgca tggtcagaag gatgtttttg ctcttccaca ggtttagaag gatcgattgc
     3841 atcatgttgt tctggaactt tagattctgg agttcctgga tgatgaggtg catgcttttc
     3901 tgcatcatgt ttcttctctt ctactccttg ttgaacgtta gggactactg gatctggatg
     3961 tgcagatact ggtgctgttt tgttcttttc atcgttcaca ccatttgatt ggtttggtgt
     4021 cgttgggtct ggatgtgcag acactggttt ttggttgttc attttattgt tattattttt
     4081 gttattgtta ttcatggatg tttcctccta agatttaaaa tagtgatcta agattttcgt
     4141 ttgtttcaga taaaaaccga tagcgacgcc gatcgctgcc atgatcaaga tcaataaggt
     4201 cttgaaaaag cccacggtga tccataaaac ggctaatatc aatccaagta ccccaaagat
     4261 gatagggagc ttgtatgtcg aaaatagttc tttcattttt ggtccccccc tattctactc
     4321 gtttgcggtc attgcttcgt ttagctggtt cttcaaagcc tgtgtatttc accgatactt
     4381 tcactggctc ttttgttccc aatatgtgtt gtacttcatt ttcgatttct ttcatcaatg
     4441 ttcctgtttt accaatgagt gctgaagttc gtttcaactc cccttttaca ttgacggcga
     4501 tcttgttttt cgttgctttg acatttactt ttggtgaatg gatgaattca tcttcatgca
     4561 agtgagaacg aaccattcct tcaattgctt ttttatccaa cgtcaactcc ccatcaccat
     4621 gtttcagtag aaagtttccg attgtttttg gataaaacag gaaaacaagt aacgtgataa
     4681 ttgtcaaaat agcaaatgca acggcaagcc aaaatacata ttgttgcacg tatgaattag
     4741 tgatggaaaa gaatcgaaac ttctctagct gaaaaggcaa tggataggtt gcacttaata
     4801 gtatcgcaaa caaaagcaca gatagcaaaa gtaatgttcc gatcactccg atcgttttta
     4861 cacctttatt cattttcgca cctcctcata ggggtgaaac gatcaacttc ttataagttg
     4921 atcgcttctc caccagttac tccgtaaatt tgggctgtaa cataacttgc tttattcgat
     4981 gctaagaaaa cataaactgg tgctaattct cctggctgac ctgcacgttc tagtaaactt
     5041 tgctgaccaa attcagggat cgaatcagaa ggttgtcctt cgtctagttg taatggtgtc
     5101 cagattggac caggtgctac accatttaca cggattcctt taggtgcaag ctgttttgct
     5161 aaaccgatcg taaagttagc gattgaagct tttgttgctg cgtaatctaa taaatgttcg
     5221 cttggattga atgcttgaac agaagttgtt gttacgattg ctcctcctgc tgggatatgc
     5281 ggaacagctt ctttcactaa agcaaacata gcgatgatgt ttatcataaa tgtgtctttg
     5341 acttgctcaa gtggtaactc agcaatagac tgacaagaaa tttgctgtcc tgcgtttagt
     5401 accatcgtat ctagcccgcc aagttcactc acggctttat ctatgatttc tttaggtgct
     5461 tgttcgtcac gaagatcaaa aggtagtaag acaatcttac gtcctgcaac tttcacgtat
     5521 tttgctactt cctctgcatc tttttcttct cctgggaaga attgaagagc gatatctgcc
     5581 ccttctctag caaatgcaat cgctgctgca cgaccgatcc ctgaatctcc accagtgatt
     5641 agtacacggc ggttttctag ttgattattg ccttcatagg attcttctcc acaatcagga
     5701 acaggaatca tattctgctg taaagctggt gtatcctgat cttgtttagg aaatttctcc
     5761 gtatggtaca agtttcgcgg gtcgattaat tttggttctg tcattgctgt attcctccta
     5821 gtttttaaat tgtattgcca aaacaaattt ctttgtttga tacctgtttt aaatagattc
     5881 tttaatttgt ataatctttc cggtataaat ggtaacctaa aactactgct gctatcaaag
     5941 agccaaagac tacgagtata aagcctagag cagaactttt acccggtaat cctcctgtat
     6001 tcattcccca aatccctgcg aacaacgccg gaacggaaat gattaatgca gctgaatcta
     6061 gatatttcat gatatgattt agattattgt ccatcatccc gttgaataga tcaccaatac
     6121 tctctaataa atctcgatag atctcgatca ttttttctgt ttgtcgctgt ctcaaatgga
     6181 catcatagag tagctgttca tcgtctaaac gtttgatgaa agtttcagaa ttccataatt
     6241 gatccattgt ttcccgttga tctcttaagg tatgatcgat atatacgatg tctctttcaa
     6301 gatctgcctg tttatacagt tcttcatttt ccgttgtttt acgagctgct ttatccaaag
     6361 cgtcaattcg ctttttcatc tctcttagtt ctttcacgta atgggtgtaa atatctaata
     6421 atgtatacgt gatgatcttt tcaaacgagt ttaaatgatt tccatgcttt gtcagcagcc
     6481 gatccacaaa ttcggttttt ctatcgatac aagtaatcaa gagattatcg gaaagtacaa
     6541 aagatattgg cgttaatttt ttttcgatac gattatctga ctgatctaga ttaatcacta
     6601 ccaaagaaaa tggatgctct agtttcgtct cataaaggcg ctccatccga gaaacttctt
     6661 ccggatattc ggttcctacg aaaatatcat caggaagctc gaattcttcg attgcttgct
     6721 caatctcttc gggctttgca ctatcaagta cgagccaatt atattttttt tcatctgttt
     6781 ctattacatt cttttcttca tccacaaagt agtatctcaa tgtatacctc ctttttggac
     6841 tttctcgaaa ctattagctg aagaagcagt tctttaattt gatattataa tcgagtactg
     6901 cgatttcatt ttctaatgaa aagctgtcaa gctgtttgtt gaagtccatg ttcaatcgtt
     6961 taccgaaata attttgctct aatttagatg tatagatgtc caataatgga tgctcgattt
     7021 tagtcgcata ttctccaaat tccgaaacaa aaggaatgat ttgaaaaaag ctgccttttg
     7081 caaaagcacc tttgtccctt agaaatggat cggaatcatt gataagcgcg agtgcgattt
     7141 ttgtattttc cccaaccttt tcaattaacg tctctactgc atttttagac attgtaattt
     7201 ccattcctac tccccctctt cagttgttgt cttctattat ttaacaacta catctgtttt
     7261 tggttgaact tttgcatatt ttggatattt agggaattta gctacacaac ggaaagccgt
     7321 catccctgca tatttttcgt aatatttctt cgttggccct tcaaaatcct tgctccacgg
     7381 tttcacatgt cctgaaaaat ggataatgta aggattgttg cgagtttctt cgtattcacg
     7441 ttcacctgct gctgttggat gtttttttgc ttttgccatg atatagcctt gtgcattcca
     7501 acgcggatga agtggtaacc aacggtcatg taagatagca tttaatgcat cttggtcatg
     7561 gaatcgtaat ttttctggat tatgttcgat gaaatctaat actttttgcg taatattttc
     7621 atctaaccat tttttcacat tgatcagcat caaacctgag ttgaaataac gcattgattt
     7681 agctgaaatt ttcatttttt ccagtcgttg atggaaccca gcgtcttcaa cagctgctac
     7741 gattgaatca ccgaagtcaa gacgccataa tttagagata tcttgtaaag cgatcatgtc
     7801 gcaatccata tataagatac gttcaacttc tgtgccgcgg aacaattccg gaatcgcaat
     7861 acgataataa gctgtttctg gaatacgatc gctgattacc acatttgtaa agaattcttt
     7921 attgattttc aagaattcca catctgagtt cattcgtgcg tttttcactg agaagcgtaa
     7981 aagctgttta ctttcaaaat cgatatcatc gtcaatcaca tagaaccgaa caaatttaga
     8041 cgggttgcaa ttatctaata ctgagacaaa taatgcagct aaatgtggta cgaattttgt
     8101 gttacaactt gcgactactg ctatttcttt tttattcatt gatctcgcct actttcgtta
     8161 gagtactgtg tgctaatttt tttaagtata aattggtata cggatgatct ttcaacgtat
     8221 tccaaggttt atcatgtcct gtaaagtgaa cgatcgaagg tttttcattt ccttctttat
     8281 actgacgttc atatttttca tttggtgctg gatgacgttc aaaaatcagc gaagtctgca
     8341 tgttccattt tggatgaagt tgttcccaat cttcataaag tacagcattc aaagcatctt
     8401 ggtcgtgata gatgattcga tcgccatttt cactgaggta atggatagtt ttttctgtaa
     8461 tttctttttt attccattga tcaatgtcaa tcaccatcac tcctgaattg aagtacaagt
     8521 catcggaatc gattcctaat ctttccaaag ctttcacttg gcctggatcg ataatagcgc
     8581 cgattgtttt tccatttaac ggctcatcat atagttttac gatatcgtct agtactaaca
     8641 catctgaatc taagtaaagg actttcttat aatcctcttt agctaataag tttggtaaag
     8701 aaattcttaa gtaagcggta gttgtgatat ggtcactgac taggaagtct tcgtagatat
     8761 ctttttccac tgtcaggaat tggatacttg cattacttga atatttattt accgtttctt
     8821 ccaatccttc tttactatat tctgatagac catcgtcaat cacataaaat tttatgcgtc
     8881 ttgttttatt gcaattttct aatgcagttg cgatcatcac gctcaagtaa ggagcataat
     8941 tttcgtctga agcagttacc acaggaacta cgccgtatct cttctccatc aaaatcactc
     9001 ctttttctaa tctttttcta acctgttcta catctagaat attcctcatt tggttttaaa
     9061 gcaaaatata tgctttgttt tcattgaata ttttaaaatt tttttatcac aaaacgcatc
     9121 ggtaagcgct aactattcgt ttttcatggc tttttattca aaggtattga
//
LOCUS       DAZKPO010000102         8877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000102 DAZKPO010000000
VERSION     DAZKPO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.104"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(481..705)
                     /locus_tag="ACG8NZ_002571"
     CDS             complement(481..705)
                     /locus_tag="ACG8NZ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540184.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            896..3673
                     /locus_tag="ACG8NZ_002572"
     CDS             896..3673
                     /locus_tag="ACG8NZ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0540185.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3754..4173
                     /locus_tag="ACG8NZ_002573"
     CDS             3754..4173
                     /locus_tag="ACG8NZ_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0540186.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4185..4655
                     /locus_tag="ACG8NZ_002574"
     CDS             4185..4655
                     /locus_tag="ACG8NZ_002574"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0540187.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4672..5451
                     /locus_tag="ACG8NZ_002575"
     CDS             4672..5451
                     /locus_tag="ACG8NZ_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0540188.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5438..6259
                     /locus_tag="ACG8NZ_002576"
     CDS             5438..6259
                     /locus_tag="ACG8NZ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0540189.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6276..7325
                     /locus_tag="ACG8NZ_002577"
     CDS             6276..7325
                     /locus_tag="ACG8NZ_002577"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0540190.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7338..8342
                     /locus_tag="ACG8NZ_002578"
     CDS             7338..8342
                     /locus_tag="ACG8NZ_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0540191.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2965 a         1469 c         1837 g         2606 t
ORIGIN      
        1 tgttcttgca atcttttttg ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta
       61 attgattttc aaagaaggag tatgaaaatg agaaaaatat ttattcgttt attgtgggta
      121 ctatcttaga ggaataaata atagtggaga aaactatggt ttggcgcaga ttctatcagg
      181 tttgatggaa cgaagacaac ctcccgacat caagtcaaaa gctacttgag attttgatct
      241 gaaaataaaa aaagaaaaca aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca
      301 atgggaaaaa actaagagtg gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct
      361 ctttactctc tgaaagcatc ataactcgtt tttcctcatt atgccaataa attgttgtta
      421 taccacatat aaggatttgt aatagctaaa aacagacagg aaagttgaaa tctgtgaatc
      481 ttattttgta tggaaggatt tatagaacgc aaaaatcaag ataaagatga ataaaaatcc
      541 tgtttcggct atatattgcg tatctacgcc aataaaacga gtttgttctg tcatagccat
      601 ataaagcaaa ggcaaaaata caactacagc gagataagat ccgatgattt ctcgaaaagt
      661 tttccaggaa gtatgtttcc tgtttttata tttatccgat ttcattgaat agtcatagcc
      721 tccttattct ttcatatgtt gaaattccag tataaaaact ggaaggaact gtacgttttt
      781 tagaataaaa attataaggg ataagtaggc tgctgtctat aataagatat atttttcaat
      841 tctttattaa agattttatt gacaaaatga atccgttttc tatgatggag gaggggtgaa
      901 taagttgttg aagaaggaaa ttttatgtta cttagagaac cagacagcct ttttcgattt
      961 agaacatatg aacgaggtct ttacagcaca aaggttagct gatcattttg gggtaaaaag
     1021 aaacacagtc agtcattatt tgaaccagtt aaatgatgag caactactag tcaaaatcaa
     1081 ttctcgacca gtgatttatt tccacaaaga agcttttgaa aaacaatttt ttaagttacg
     1141 cacgaatttt tatttttcta tcgatgatct caaagaggaa cagccttttt tcgcgcaacc
     1201 aaaagactta ttctctttta tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca
     1261 aatcaaaaca gctctttact atccagatgg cggattacca cttttgatta caggcgaaag
     1321 tggcacaggt aaaagttatt tagttaattt ggtctatcag tattgtctgc ttcatgatct
     1381 gctagaagat tctgcaccat tcatcacttt taattgtgca caatatgcag ataatcctga
     1441 acttttgact agcaatttgt ttggacatgt taagggagcg tacactggtg cagaagaaaa
     1501 taaaaaaggg gcttttgaag cagcagatgg tgggatcttg tttttagacg aggtacatcg
     1561 cttaacacca gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg tgatttatcg
     1621 tatgggagaa cctaatttgt cacgccggat caaaacacgt ctcttttttg ctacgactga
     1681 agagattact agtcatttct taacaacttt cattcgaaga atacctatcc gaatcgaact
     1741 acctgcactt aatcagcgta gtcgtaatga acggctagaa ctgatttact ctttttttct
     1801 cgaagaacaa agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt tgtcactttt
     1861 aacaagtcag aaattatccg gaaacatagg agagctgaaa aatatcgtca aagtttcagc
     1921 agcaaaagca tatgctgaac agcgtgaaca gcctgaaatc catgtcacga tccatcattt
     1981 gcaaaaagaa ctacttgcgc agcctattac taaaaataca gcacagcaag gaatctatat
     2041 tacgcaagac cagaccttag agcaattaat ggagaagcaa cagcccgaac agcagcgaat
     2101 cgttaaaagt tttgagcaaa tcctaattga tttcaagaaa aataactgtc tgctatattc
     2161 ttgcgaagga aaattaaagc aagaagtgat tcaattattt gattttttgc tgtttgagac
     2221 cagtagacaa gaaaaacacg agctgctggt ttattggaca caatctatcc gagagacctt
     2281 tcgacaaatg gaaactgcct atcaaatcaa attcgatgga aacagtgtct atgccttgag
     2341 ttactatctc tatcagcgaa gcactatcaa atggttaccg gaagaacatg atattatcca
     2401 actaatcaaa gaattagaac aacaagtttc tcagtcttat ccatctagtt atcactatgt
     2461 ccaacgaatt ctggaattaa gtaagatcaa attagatatt gaagacacaa gtatggatcg
     2521 gattttactg acgatctatc taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa
     2581 agcgatgatt gctgctcacg gttatgcaac tgctagcagt atggcaaatg tagtaaatcg
     2641 tttattacaa gaggatctat ttgaatcatt tgatatgcca ctagatgtca caccacagca
     2701 gattgcttca gaaatccttg attattgcga aaacagcgat gtttcaaatg gattgatcat
     2761 attagtagac atgggatctc tgaaagaaat ccatcagttt tttaaaaaac agttatccgt
     2821 accgcttttg attttgaata atgtgacgac acctttagca atcacagtag gggaatgcct
     2881 gcaaaaaaat gtatcattag aagagatagc agaagagact gttcgacaga tccagccaga
     2941 atggcgactg ctctatccag aggaaaacaa acccaaagca ctcattacta cttgcttcac
     3001 tggaatcggt acggcggtcc acttaagtga actgctagaa aaaagcttac ctacggcttg
     3061 tcagttgaaa atcatcccat atgaatatca gcaattaaaa gataagaaaa acagtgaccc
     3121 attattttcc atatacgaag tcgtaggaat gattggcacc acagatcctg atatcaccaa
     3181 tatcgactat ctttcattag aagacctcat ctcgggggaa aagatgtctg tactagctga
     3241 atggttggac actactatga atgcttcaga gaaggaaata ttcaatcagc gcatcattcg
     3301 taatttttca ttggaaaaag tactggattc cgtgactatt ctagatacag agaaggtgat
     3361 gggagaaatc gatcatttta tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa
     3421 tcccaaaaaa ttagcgttgt atgtccatgt gagctgtctc attgaacgat tgatccgaca
     3481 aatgccaatc gaaacctatc aaggattaga gaaatggaag cagtgtcaaa aagaagggtt
     3541 atctgctatt aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca tgatccccaa
     3601 ttctgagatt gcctatatct atgatatttt gagtgaagat acagagtttc tctcgataga
     3661 agaagaattt tgaacataag caagttgttg ccactaattt tggcatgcaa cttgcttttt
     3721 tagtatgtgt aagtgaaaag aggagatgaa aaagtggata gagaaattat cttagcttcc
     3781 catggaaaat ttgcttcagg gatattagat tcattagagc tgatctatgg gaaaaatcat
     3841 tctattactg tattcgattg ctatactgat gagaattttg atttagcgga aacagtaagg
     3901 cagcttttta tgaggtacaa agataaagaa atcattgttc tgacggatct ttttggcgga
     3961 agtgtcaaca acgaattctt acaatatatc aatcgggatc atgtctatct cgttgccgga
     4021 ttgaatttgc cactattgat tgagctagta tctcggttgg acgctgaagt agagacaact
     4081 gttttgatcc agcaggtgct aactgattca aaaaaaatga tccagttttg taacgagcgg
     4141 ctcaatcaga aaatagaaga agacgaattt taggaggaag aaaaatgatt gtattgacaa
     4201 gagtcgacca tcggttatta cacggacaag tagctttttc atggacacaa acaataggcg
     4261 cagactgtat tttgattgct aatgatgatg tacctacgaa tgagatacga aagacaacga
     4321 tcaaattagc caagccacaa ggagtcaaac tagtgatcaa atcaattgat gactcgattg
     4381 ccgcgttgaa aagcggggtg acagataaat ataagttatt catcgtagtg gaatcgatcg
     4441 aggatgcata taagctggca gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga
     4501 tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt aaatatttta ccagaagagg
     4561 aggaacgctt gaaacgatta attgacaaac aaatagaagt agaaatccgt caagtaccag
     4621 cagataaaaa agtggatgta agagaagttt tgtaagaaag gaagaacctc aatgattgta
     4681 caagcagtct tattaggaat tgttgcattt gtcgcacaat cagaatatgc actagggaca
     4741 tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta
     4801 aaaactggga tcattatggg cgcaacttta gagttagcat ttatcggatc gttctcagta
     4861 ggtgcttcga ttccacctga tgttgtgacc ggtgggattt tagggacggc ttttgctatt
     4921 acagctggag ccggtacaga aaccgcgctt ttactaggtt tgccgattgc tacgttgaca
     4981 ttgatattga aaaatattta cttagggctt ttgattccaa ttatgaacca taaagcagac
     5041 aattatgcag aagaaggaaa ttataaagga gtcgagcata tgcacttgct tgccggtatc
     5101 ggtctttcct tgatgcttgg tgttgtggta atggtttctt atatggtggg aagcaatacg
     5161 atcggaaatc tattaaatat gattcctgac ttcgttcaaa aggggttgtc agtagctaca
     5221 gggttgattc cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt
     5281 gcaccatatt ttttcttagg ttttgctata gcttcttact tagagattcc tgtgaccgga
     5341 gtagctattt ttggtgcgat tttagcagtt gtggtagtca atattatgaa tgtccgtcaa
     5401 ctaccacttc aaacaactgc aggggaggta gaagaagatg aagaattctg agatcaaaaa
     5461 cagcaaaata acgaaaaaag agctaaatca cgtattttgg cgttcttttc aaatggagtt
     5521 ttcttggaat tatgaacgtc agatgaatat ggcttatgtt tatgcaatga ttccgatttt
     5581 gaaaaagtta tatcagtcga aagaagaaat ggcagcagct ttgaaacggc atctagagtt
     5641 ttttaacacg accccacata tcgttacact gattttagga atcacggctg caatggagga
     5701 agaaaataag gatgatcctg aatttgatgt gactgcgatc gatagtatca aaacatcttt
     5761 gatgggacct cttgcagggt taggagattc tttcttttgg ggaactttgc gattgattgc
     5821 cacaggtgtg ggtacctctt tagccatgca aggaaatatt ttaggcccta tcttgttcat
     5881 tttgattttc aatattccgc acattctgtt tcgctatcta gcaacaggtt ggggatatcg
     5941 cttaggaacg ggttttttga aaaagatcca ggcaaacggt atgatggaaa gtttgacatt
     6001 aggcgcgtct attattggac tgatggtcgt tggagcaatg actgccacga tgatcaccat
     6061 caatattcct ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa gtatttttga
     6121 tgatatcgtt ccaaatatct tatgtcttgg agcatttggc atagtgtttt atctattgaa
     6181 gaaagaagtg aaaccattaa cgatcttgat cggactagct ttctttggaa tttttggttc
     6241 gtggataggg attttttaaa aaaggagcag agaaaatgtt tacgatgcaa gattatatct
     6301 atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa tgatttttcg acaagagaac
     6361 atatgaaaaa aacagtgaat ttactgatct tagcaacggg ttcatcttat aacgcttgtt
     6421 tagcagcgaa gccagcattg gaatcttatg gtgatcttac tgtcgatatt caagaacctt
     6481 tttatttttt caattatgga aaactttctc cttcaatcga tactgtgatt gctgtgtcac
     6541 aaagcgggaa aagtgcttct actgttgaag cagtaaaaat gatacaaaaa caagggttac
     6601 ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc tttagaagct gcacaaatta
     6661 ttgatttagg tgccggggta gaatcagtag gcttcgttac aaaaggctat tctgcgactg
     6721 ttttacagtt actactctta gggataggaa ttggaataag taaaaataaa atcagcaaaa
     6781 aaatagaaca agactacatg caacagttgc gcaaaataat caaccattta ccagcgatta
     6841 ttcaaaaaac ggaagaattt tttgatggat atcaaagttt atttcgttta gcacagcgtt
     6901 tcgtcaccat tggatatggt ccaaactggg gaacagctaa agaagcagag acgaaattga
     6961 ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga agcttatatg cacggcccat
     7021 acctggaggc agatgcttcg catttgctat tctttattga aggggaaagt gtaaataagg
     7081 aacgttcgca aaagttgcaa agatatatga gtcggtacgt tggagagact ctcaccatta
     7141 ccacaaaaaa agcaagaaac gagaaaacac ttggattagc aatagaatgt gatgaatatc
     7201 tgtctgtatt agcgcttgtc gttccgtttc aactattcgc ttataaaacc gcagtagcta
     7261 aaggtattga cttgaacaaa aagatttttg aagactttga tacagtcttg aaaagtaaat
     7321 tataagaggt ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa gcaataaaag
     7381 gcgcactagc tttacgtccg caagtagaag aaatcatcga taaactttat gcggaaaaat
     7441 ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca
     7501 cttatatgaa tgggaaaagc aacttgcctg tctttgtaca gcatgctgca gaatattaca
     7561 ctactggaaa caaaagatta acaaaagatt ctatcgttat tttgtcctct gttaccggta
     7621 caactcaaga agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg acactgattg
     7681 gatttatcga taaagcgaac agtaagttgt ctcagctatg cgattttgtg gtcacttatc
     7741 cagctcctgg aacagaacaa atcaaatttt ttatggcagc agatcgattg atgtatttga
     7801 atggagaatt cgaagcttat tcagaatact atgaacagct cgaacgttat ttaccaacag
     7861 ggttagtgga agcagagaaa aaagcagatg catttggctt atcatttgca gaaaaacatc
     7921 gacgtgatag tatgcattat tttattggag cagggaatca atggggagct gtttattcat
     7981 atgccatgtg ttactgggaa gaacaaagct ggcttccatc gaaatctatc catgctgccg
     8041 agtttcttca cgggacgtta gagatcgtag aagaaacaac cccagtcaca ttatttttag
     8101 gcgaagatga acagagagtg ttatctgaac gtgtggctaa acttttgcca aaaatttgca
     8161 gcaattatac attgatagac acaaaagact atccagtaga aggtatcagt gaaaaatatc
     8221 gcggacgtgt tttgtctttc ttattgatgc atgtagtgac acaacgcatc gatgctcatg
     8281 tggagcagtt gaactgccat ccattagaga tacgccgcta ctatcgtcag tttgattatt
     8341 aggatagact ggataaaaag aatataaaaa ataaagagag cagcgataac tgctcccttt
     8401 atttttttaa aatattggca tcaattacgt tataaaacac attttttgca ttatctacag
     8461 ttcaacttat aatctgttta atgggacatg tgggtgccaa ccattttttg ttatgcaata
     8521 gtactattta gttgttgcat ggatccgtag taaaagtcca agtacaacga gttgcgccag
     8581 tttacatagc gttttgtatc cagaatgttg actaaaacaa aagttcctat gtcaaccgtc
     8641 aacagagaca atctgtccgt tgcaggacct gacagattta gaaatgttca atctgtctct
     8701 tatgtatttt aaatcaatac tcactaggag aagtttccta acctcctaat agtaatgctg
     8761 gtagtaacat tgtcaaaact gcttcctctt aggaacaagc aaagcataac gagtgtaaaa
     8821 ataaaatgta gactattgta acatatcatt gattatttca agtaaaaacg ataaaag
//
LOCUS       DAZKPO010000103         8857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000103 DAZKPO010000000
VERSION     DAZKPO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.105"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8NZ_002579"
     CDS             14..1141
                     /locus_tag="ACG8NZ_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0540192.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG8NZ_002580"
     CDS             complement(1359..2501)
                     /locus_tag="ACG8NZ_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0540193.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG8NZ_002581"
     CDS             complement(2506..2691)
                     /locus_tag="ACG8NZ_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0540194.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACG8NZ_002582"
     CDS             complement(3045..3290)
                     /locus_tag="ACG8NZ_002582"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540195.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACG8NZ_002583"
     CDS             complement(3284..3685)
                     /locus_tag="ACG8NZ_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0540196.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG8NZ_002584"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG8NZ_002584"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0540197.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="ACG8NZ_002585"
     CDS             complement(5703..7469)
                     /locus_tag="ACG8NZ_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0540198.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="ACG8NZ_002586"
     CDS             complement(7482..8276)
                     /locus_tag="ACG8NZ_002586"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0540199.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..>8857)
                     /locus_tag="ACG8NZ_002587"
     CDS             complement(8286..>8857)
                     /locus_tag="ACG8NZ_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0540200.1"
                     /translation="NDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFF
                     SAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDG
                     TVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFL
                     IVGLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         2870 a         1697 c         1181 g         3109 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca tcattat
//
LOCUS       DAZKPO010000104         8591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000104 DAZKPO010000000
VERSION     DAZKPO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.106"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..1516)
                     /locus_tag="ACG8NZ_002588"
     CDS             complement(50..1516)
                     /locus_tag="ACG8NZ_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0540201.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1903..2499
                     /locus_tag="ACG8NZ_002589"
     CDS             1903..2499
                     /locus_tag="ACG8NZ_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0540202.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2557..3039)
                     /locus_tag="ACG8NZ_002590"
     CDS             complement(2557..3039)
                     /locus_tag="ACG8NZ_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540203.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3145..3342)
                     /locus_tag="ACG8NZ_002591"
     CDS             complement(3145..3342)
                     /locus_tag="ACG8NZ_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540204.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3637..4395
                     /locus_tag="ACG8NZ_002592"
     CDS             3637..4395
                     /locus_tag="ACG8NZ_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0540205.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4455..4883
                     /locus_tag="ACG8NZ_002593"
     CDS             4455..4883
                     /locus_tag="ACG8NZ_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540206.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4929..5001
                     /locus_tag="ACG8NZ_002594"
     tRNA            4929..5001
                     /locus_tag="ACG8NZ_002594"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4962..4964,aa:Gln,seq:ctg)
     gene            complement(5151..5522)
                     /locus_tag="ACG8NZ_002595"
     CDS             complement(5151..5522)
                     /locus_tag="ACG8NZ_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0540207.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5737..5922
                     /locus_tag="ACG8NZ_002596"
     CDS             5737..5922
                     /locus_tag="ACG8NZ_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540208.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6005..6193)
                     /locus_tag="ACG8NZ_002597"
     CDS             complement(6005..6193)
                     /locus_tag="ACG8NZ_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540209.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6217..6480)
                     /locus_tag="ACG8NZ_002598"
     CDS             complement(6217..6480)
                     /locus_tag="ACG8NZ_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540210.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6638..6895
                     /locus_tag="ACG8NZ_002599"
     CDS             6638..6895
                     /locus_tag="ACG8NZ_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0540211.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6966..7157)
                     /locus_tag="ACG8NZ_002600"
     CDS             complement(6966..7157)
                     /locus_tag="ACG8NZ_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540212.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7298..7618)
                     /locus_tag="ACG8NZ_002601"
     CDS             complement(7298..7618)
                     /locus_tag="ACG8NZ_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540213.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7641..8075)
                     /locus_tag="ACG8NZ_002602"
     CDS             complement(7641..8075)
                     /locus_tag="ACG8NZ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0540214.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8410..>8591
                     /locus_tag="ACG8NZ_002603"
     CDS             8410..>8591
                     /locus_tag="ACG8NZ_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0540215.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2723 a         1695 c         1361 g         2812 t
ORIGIN      
        1 aaaacaggaa ctgctgcaaa taagcaattc ctgtttttat gttcattgat taatgaatag
       61 agcccattag ttttttgatt ggttctttca cagtaaatag caagatacca ccgacgattg
      121 ctactgcacc gataataccg aagtaagcag attctgttcc cggtgtgtag aagcgtgcaa
      181 tttgtgcatt gattgcttgt gaagaagcat cagctaagaa ccaaatagcg attgtttgtg
      241 attcaaatgc tcgtggtgct aattttgttg tcacagacaa accaactggt gaaagacaca
      301 attcagcaat aaccacgatg aagaaacttc ccaacaacca caatggactt acttttccac
      361 ttgtaccata aagcataccc ggcaacatca atagcaagta cgataatcct gcaaataaaa
      421 gtccaagaga gaatttcact actgtagatg gttgacgttc accaagtttc gtccacatcg
      481 ttacgaatac tggtgtcaga ataaagatga aaattggatt caatgattga taccagcttg
      541 cttggatcgt tacaccaaat agcgtagatt gtgtacgttc actagcaaat aaagctaaga
      601 tagatgaacc ttgttcttgt aatgaccaga aaatgattgc tgcaaggaat aatgggatat
      661 aagcccatac tcttggtttt tcttcagcag ttacatcttt tgaagtcagc attttgataa
      721 agtaatacaa cggtaagata atccccaata cactgattgt attgacaaaa aagtcaattg
      781 tcagatgacc agttagttgt gccccgccga atactgcagc gataacaaca agtgcgatga
      841 tcaagttacg gatgaatcct ttacgttctt cagttgtcaa aggattcggc ggtgttgtac
      901 ccactccgtc taatgtcttg cgtccttgga tgatgtattg taataatcca aagaacatcc
      961 caatagcagc taaagagaac cctaaatgat agttatattc ttgaccgatt gtaccaacga
     1021 ttagtggtgc taataatgcc cctaagttga cacccatgta aaaaatcgaa aatcctgcat
     1081 ctcttcgcaa gtcagtcttt gaataaagat gtccgaccat gccagaaaca tttggtttca
     1141 aaaaggcggt tccgataata atcaatgcca tcgaaacaaa caatgtactt acaccaaatg
     1201 gtaaagccaa gacgatgtgg ccaagcataa tgaaaactcc accgaagaat acagtgcgtt
     1261 ttgctcctaa cactcggtca gatacccatc cgccgacgat actagacatg tagatcaaag
     1321 atccgtagat cgacatgata gctaatgctg ttgctcgtgg taatccaagt cccccgttgg
     1381 caactgtatc gtacatataa tagataagta atgcacgcat accgtaataa ctaaatcgtt
     1441 cccacatctc agtgaagaac aaagttgaca atcccttcgg ctgcccgaag aagcccttgt
     1501 cttgctgttt ttccataatc tataccccaa ttctttaaat tttcagaaaa tattatacat
     1561 ctttaccgaa aaatttaact aaaaaactta tttaagaaat ataaactaat tttctataaa
     1621 agcaagtaat ttttaatggc aatatcataa aaaaaagaag atagagattt aataattata
     1681 tatcagctgt ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac
     1741 taagaaaatc cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt
     1801 ttttgtgagc atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca
     1861 attaaaaaaa tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac
     1921 actttgaaaa accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa
     1981 gaattaacaa ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa
     2041 tcaacacgcg cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa
     2101 gaagcacttc gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct
     2161 ggtttcaaaa atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt
     2221 ctacaagaac aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac
     2281 ggaatcgcaa cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg
     2341 caacattaca acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac
     2401 tggaaactac gttcgcaatt agtattcggt tcaccagaaa caccggctga ggaaaaagaa
     2461 tacatgaacg atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca
     2521 gaggttgtga caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc
     2581 aatctctgcc tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc
     2641 agaaaatttc agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt
     2701 agaacctttt ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt
     2761 gtcaaagcta ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact
     2821 aataaaaatc attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat
     2881 gagtgtatac caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt
     2941 tcgcacagtc cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac
     3001 aacgaataaa aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc
     3061 ttctccagaa aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc
     3121 acgccgcacg tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct
     3181 tgctttttca tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc
     3241 gcagtttgtc atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt
     3301 gcatgcatat tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg
     3361 ctggaacttt tctccacatt accactgcta tttctttgga gatttaactc attgattttt
     3421 atttacagtt aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt
     3481 aaaacagctg ttgtatttag aagaattttt ttcaaaaaat taaaacatat tccttaaaaa
     3541 ctaattattt cctacatatt aaaatataat acttccattt ccaataaaaa tacatttata
     3601 ataggaaaga taaacagctg aaaagggaga tttcaaatga agaaaaaaac gttcaaaaaa
     3661 ctattgtttc tagggggcac agctgctctt ttattaagcg gtacaagtta ttacctacat
     3721 atccattctc cagaacatac aatgaacact gcacaaaaaa gttcaacaca aaaaacgaaa
     3781 aagacaaaag agaaagcaga agttttacta gatgttccat tagagagcca gtttgattct
     3841 ccttctttag aaaatggctg tgaagtcact tcgctttcta tgcttttatc tttttatggt
     3901 tatgaaacaa ctaaaaacca gttagctgag caattggatt acgtccctgt tttcaatgca
     3961 gatgggactc atggcgatcc taatgaagga tttgttggtg atatcagtgg cggagactgg
     4021 gctatggtcg tctatgttcc cccagttgcc tcccttgctc agcaaatcgt ccaaacggat
     4081 tatcacactt ttccaaaaac cgatgctcaa ctcgatgata tcaaaaaagc actatctaaa
     4141 ggaaatcctg tctggacttc tgtgactatc aattttgaag tcccagatga aactgatttt
     4201 atgacttgga ccacaaataa tggagaagta agagtcactc ctcttgttca tgcttgtgta
     4261 gtcactggat atgacaaaga aaatatttat gtcaacgatc cctatggtgt aaaaaatcga
     4321 gcagtaccga tcaacgattt ttcggcgatt ttcactgcaa tgggtggaca gatgctaact
     4381 ttggagaaaa gctagtaatt caaacaaaaa tcccttatac tcaagatacc aaacctttga
     4441 agggagcgta gataatggaa aaattcacac ataaaaaaat ggatccaaat gaaattccga
     4501 ttatttttgt ccgtgactgc aagggaaatg ttcaaggtaa agtctctatt aatgagtgga
     4561 acgagcgtcg cagacctgct accctcaatg aactggaaat caaattgtat cgtcaatctc
     4621 ttgtttatta cgcagatcaa gagtacgaga aagccaccga tttattgaaa tttctaattg
     4681 ctcgaacaga gtatactcgc tttgaatata ttgaacgttt agcaaacatc tatcatatta
     4741 tgaatgaacc tgtcaaagaa tatcagttat tggacaccgt cttgtcagta gcagaactga
     4801 ttgcccttcc tgctggatta gaaaagaaac tagtacgccg ccttttacga gtaaagcaac
     4861 aactatccga tcaagaaaaa taacttgcgt ttttttagga aagtgctatg attattttgt
     4921 tgaataaatg ggatgtagcc aaactggtaa ggcacttgac tctgaatcaa gcaattgtag
     4981 gttcgaaccc tgccatccca atttgatgtg cttgctgaaa aaatgaaccc cctgtaaaag
     5041 aacaatcttt ttgcaggggg attattgtat tatttttact acttgtattt agtttacata
     5101 attaaattat gaataataaa atcgggcgta ttcttaatac aaaaactgat ttacccaata
     5161 attgctaatg ctttctccgg aactttttct ctaggaactt cctcatacgt ttctatgtgt
     5221 gctcctttat tgctaatttt caaataatga ttttctttta acacagaaag accattaaat
     5281 tcaaccgttc gttctttccc attggcatca gcagctttct gcacatacct tgctgtacca
     5341 tattcattta cttcctctgg tttttgtgtc tttacataga cctctccttt ttcaactaat
     5401 ggatttagct gatcaagtat accagggatt tctccataac ttccttcagt ataatatttc
     5461 agtccaaata gcaaaatacc actaatcgcc gccagtccaa tcaaatattt aatcactttc
     5521 atctttctac ctccttgtta tatattcagt atatgggtca gttaaagaaa ttccatccgt
     5581 cccaagttgg agatttttct cctactttag aagcgagaat cgagtgtttt tttagtaaaa
     5641 atgatcgtta tttgatatta ttgacgtgtg gataaatcag tttttctcgt tttgtccatg
     5701 taaaaaatca tgttgatagc aaaggatgtg ttcaaaatga atcaagaaga attatttcaa
     5761 aaagtcaagg aaatgattaa gaacggcaat ttcgatggcg caaaacgatt tatcgaagaa
     5821 cataaagaac aattaggacc ttataaagag aaagcccaaa atcttttaaa agatgtaaat
     5881 atcgacagtg tcaaaaacaa atttaaaaat ctattcaaat aagtaaaact ttcacttttg
     5941 taaaatcaaa ggaggacaat gctgctgcat cgtcctcctt tggttctttt ctgaatactt
     6001 catactaaaa taaacgtgtc ggatcgtaat gataagtcac attgccgttc agtgaggctt
     6061 ttcccaccac atatttacga ttctccaact tcagccacgc ttctcgaaaa gccattaatt
     6121 cttctctttt tacatcaatc ttttcgattt ttccatcgat caaatcttga ataagttgtt
     6181 catatggatt catttttttc ctccatctgc tgtcaatcaa taagttgtaa aaataggttc
     6241 atatgctttt ttggtatcta gatcttctct gactttgact gtcgtataaa taattttttc
     6301 tactgggata ctataataac ttccttcacc attattgaat cggtgaacgt ctttctttgc
     6361 taaaatacca tttattttat tcgtcagtac acgttgttcg acactgttac ttacaacata
     6421 gtaaatcacc cgaatattat tgatcaaatg caaatcgatt tctaacacta catgatccat
     6481 gaaaatccca ctccttcttt cactttgatt atagcaagtt tctcctctaa tttcaaaatc
     6541 gatacagatt ttaccggata aatccacaaa actgcttgcg ccatttcgca caaagtgtta
     6601 ttttgtatca tggactttat ttatcggagg caatagtatg atcgcgatcg gtcaatttgt
     6661 tttttatatt ccttttttta ttatgataag cattcttttc tattacataa aatggacaaa
     6721 aaagaagttt tcagttttac tggcctcttt acctgctgta tattttactt atcaaatttt
     6781 ttcttttagg cattgggaaa caacgtctgt attaataaca cacatcatcg aattgacgct
     6841 ttcagtaatt tttcttatta tttggatcta ttttttatat aagaatcaga attgaatccc
     6901 tcgcatgttt aaaaagaaaa cgattaaaga tgcagcaaat ttctgccgat ctttaatcgt
     6961 tttttctatt ctgttatacc aaaaaaatgt ttgaatcggt gaaggatcgt tccttgtttt
     7021 aattctataa attcttgtgc gatcgggtac ttttcttcca aattttcttt gttcttttcg
     7081 atcaggcttt tcgcttcttc atatttttta tctgcacata gtgcttttac ttcctgtaat
     7141 acttttcctg cttccatttg cctcaccctc ttgccatttt attgctttaa gttttattta
     7201 agttatacgc attattctag tttcatccca aataaccttt tttcattttt actcctcgaa
     7261 ctaatcagtt ttctaaaggc tgattggttc gtctttttta tttttcctcc atttcattct
     7321 gctcttctgt taatttatct aatgctttga ttgatgctgc ggctcttgca taatcatagc
     7381 tgtataattc cataaattta tcaggcagaa caaaccacgc tggatgataa ccgttagctg
     7441 tgacagggaa tcctttatct tgttggttat ctccatccca tctcagagca acaccttttt
     7501 ttcctttcca ctttactaaa gcgatcgagt atcccttctt tgtttgtgtg ccgtcttcaa
     7561 taattgctaa aatttctatt ttttcttttg gcgcacggac ttcttgagga cgaaccattt
     7621 tctattcctc ccttatctca ttacttcacg accaacacac tgcaaggggc atgtgatacg
     7681 acataagaag cggtcgatcc aactaatgct tgttgtatag caccttttcc tgtcgctccc
     7741 ataacgatca aatcaatatt ttcttgtcca gggatgacat tggcaatcaa atttcttgga
     7801 tctccagttt caactattgc gtggatctca gcaatgccgt actctttggc atcattgatt
     7861 tttttcagca tggctacttc tgttctttgt ttttcatctg catagatttt tgaaaaggca
     7921 taggcactat gggtcagctc aactttgttg atgattgaca cgataaataa gtgggcttca
     7981 ttgtcctttg ctaagtcaag ggcttcagcg aacgctttga tcgattgtct tgatccgtca
     8041 actgctacca tgatgttctg gtatctttta ctcattggaa ccgcctcctt tttcttttat
     8101 tgtaacatct caagcaaaaa tacaaaaaat atacgcaata tgtaaataaa atgatgaaca
     8161 aaagcaattt ctttcaagtt atttatacat ttcttttctc atcagaacac ctgttctaat
     8221 cccattattt ctgcattatg tgaaattcct catcattttt cacatcgagc gaacatcatt
     8281 ctgttacaaa aaacgtggat ttgtcataaa ttgtcatgct tcttttacct atgaaatatt
     8341 ttcacgtgtt aaagtattcc tcgttgaaca caacaaataa aactttttaa cgaataaagg
     8401 agatttttca tgacatcact taaaacttta ctttttggaa caactttggc tgccggcgct
     8461 gcattcttca tgggaacgac tgctcatgcg gacgaagctt ataccgttca atcaggtgat
     8521 actctatcaa cgatttctca aaaatacgtt ggtgataact ccttgatcaa tgccattgca
     8581 gaatcaaatt c
//
LOCUS       DAZKPO010000105         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000105 DAZKPO010000000
VERSION     DAZKPO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.107"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8NZ_002604"
     CDS             complement(<1..149)
                     /locus_tag="ACG8NZ_002604"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0540216.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8NZ_002605"
     CDS             complement(593..1714)
                     /locus_tag="ACG8NZ_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0540217.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8NZ_002606"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8NZ_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0540218.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8NZ_002607"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8NZ_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0540219.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8NZ_002608"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8NZ_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0540220.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8NZ_002609"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8NZ_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0540221.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8NZ_002610"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8NZ_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0540222.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8NZ_002611"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8NZ_002611"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540223.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKPO010000106         8259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000106 DAZKPO010000000
VERSION     DAZKPO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.109"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..711
                     /locus_tag="ACG8NZ_002612"
     CDS             64..711
                     /locus_tag="ACG8NZ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0540224.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1404..2342)
                     /locus_tag="ACG8NZ_002613"
                     /pseudo
     CDS             complement(1404..2342)
                     /locus_tag="ACG8NZ_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2342..2959)
                     /locus_tag="ACG8NZ_002614"
     CDS             complement(2342..2959)
                     /locus_tag="ACG8NZ_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540225.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2973..3143)
                     /locus_tag="ACG8NZ_002615"
     CDS             complement(2973..3143)
                     /locus_tag="ACG8NZ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540226.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3491..4981)
                     /locus_tag="ACG8NZ_002616"
     CDS             complement(3491..4981)
                     /locus_tag="ACG8NZ_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0540227.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5024..5323)
                     /locus_tag="ACG8NZ_002617"
     CDS             complement(5024..5323)
                     /locus_tag="ACG8NZ_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540228.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5689..5973)
                     /locus_tag="ACG8NZ_002618"
     CDS             complement(5689..5973)
                     /locus_tag="ACG8NZ_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0540229.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6492..6977)
                     /gene="vanZ-A"
                     /locus_tag="ACG8NZ_002619"
     CDS             complement(6492..6977)
                     /gene="vanZ-A"
                     /locus_tag="ACG8NZ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0540230.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(7130..8041)
                     /locus_tag="ACG8NZ_002620"
     CDS             complement(7130..8041)
                     /locus_tag="ACG8NZ_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0540231.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2522 a         1471 c         1231 g         3035 t
ORIGIN      
        1 tgaaaaaatc agaggattat tcctcctaaa tataaaaatt taaaatttag gaggaagtta
       61 tatatgactt ttaatattat tgaattagaa aattgggata gaaaagaata ttttgaacac
      121 tattttaatc agcaaactac ttatagcatt actaaagaaa ttgatattac tttgtttaaa
      181 gatatgataa aaaagaaagg atatgaaatt tatccctctt taatttatgc aattatggaa
      241 gttgtaaata aaaataaagt gtttagaaca ggaattaata gtgagaataa attaggttat
      301 tgggataagt taaatccttt gtatacagtt tttaataagc aaactgaaaa atttactaac
      361 atttggactg aatctgataa aaacttcatt tctttttata ataattataa aaatgacttg
      421 cttgaatata aagataaaga agaaatgttt cctaaaaaac cgatacctga aaacaccata
      481 ccgatttcaa tgattccttg gattgatttt agttcattta atttaaatat tggtaacaat
      541 agcagctttt tattgcctat tattacgata ggtaaatttt atagtgagaa taataaaatt
      601 tatataccag ttgctctgca acttcatcat tctgtatgtg atggttacca tgcttcacta
      661 tttatgaatg aatttcaaga tataattcat agggtagatg attggattta gtttttagat
      721 tttgaaagtg aatttaattt tatacacgta agtgatcata aaatttatga acgtataaca
      781 accacatttt ttggttgctt gtggttttga ttttgaattt ggttttgaac ttatggactg
      841 atttattcag tccatttttt gtgcttgcac aaaaactagc ctcgcagagc acacgcatta
      901 atgacttatg aaacgtagta aataagtcta gtgtgttata ctttacttgg aagatgcacc
      961 gaataaaaaa tattgaagaa caactagcaa aagattttaa agagttattt tattttaagt
     1021 ctttataaca tgagtgaagc gaatttttaa atttcgatag aaatttttac atcaaaaagc
     1081 cccctgtcaa aattgacgaa gggggttttt tggcgcacgc ttttcgttag aaatatacaa
     1141 gattgaaaat cgtgtataag tgcgcccttt gttttgaact tagcacgtta catcaatttt
     1201 ttaaaatgat gtataagtgc gcccttttaa attttgagtg attatatttt ttgagttaga
     1261 aaaagggatt gggaaaattt cccaaaataa tttaaaaaat aagcaaaaat tttcgataga
     1321 gaatgtgcta ttttttgtca aaggtgtata ccttgactgt gcttgctgtt acattaagtt
     1381 tatttttaag ttattaaaaa agaaatagct tttaaagttt ggctcgctgt cgctttataa
     1441 agctgattga cttttgattg caaactactt aaagaaaaca aactcggact attcgttttc
     1501 ttctctttgg tttgaacatc agcaattatc ccctcttgat tgcctatttt agcttgttta
     1561 gaagaaacaa aagctaaaag ctcctcttgg gttttaaaac gctgtgtggg gcttagaacg
     1621 cccttaaacg acccttggtt tacttttata ctagcttcca cctcgaaaaa aggttctttt
     1681 ttaaaattct ctatggcttc ctggcgctga aaaataaggt ataaggtggg cgtttgaaca
     1741 cgtcctagtg aaaatgtacc ttgtacgccc ttctgttgta aatttaacgt atacaaaggg
     1801 cttgcgttca tgccgatcaa ccaatcggca atttggcgtg tttgcgcttc ttgataaaag
     1861 ggatagtaat tcattccagg ttgcaaattt tgaaaaccgc ttcggattac atctttttct
     1921 aagctattga tccatagtct tttaaatgtt ttatcttttg aaaaggcatt tgctttatgg
     1981 ataatcgacc aggcgatatt ttcaccttct ctgtcgctat ctgttgcaac aataattgta
     2041 tttgcctttt tgagaagttc tgcaacaatt ttaaactgct ttcccttatc ttttgcaact
     2101 tcaaaatcgt atcgatcagg aaaaatcggc aaagattcaa gtttccaatt ttgccacttt
     2161 tcgtcataat gacctggttc tgctaattcc actaaatgcc caaaaccaaa ggtgataaac
     2221 gtttcatctg taaatagtgg gtctttgatc tcaaaataac cgtctttttt ggtgctttgt
     2281 tttaaagcac ttgcgtaggc taatgcctgg cttggttttt cagctaaaat aaccgtactc
     2341 attaactatc cctcttttca ttgttttttc tttgatcgac tgtcacgtta tatcttgctc
     2401 gataccttct aaacgttcgg cgattgattc cagtttgttc ttcaacttct ttatcggata
     2461 aaccattcaa aaacaaatcg aaagcatgct ttaaacgtgg atcattttct ttaaatttat
     2521 gctgacgacc tttgaatttg ccttttttct tagcaatttc gattccttgt gcctgacgtt
     2581 ccttaatttt ttttcgttct gattctgctt gatacttgta caattcaatg acaaggctat
     2641 taatcaaacg ccttaaattt tcatcttcaa taccattcat tgagggtaaa tttaagactt
     2701 ccagggttgc ccccttaatt tgaatttgat tcatcaattc tgttaattct ttattatttc
     2761 gtcctaatcg atctaattca gtaacaataa caatatcccc ttcacgaata tagttaagca
     2821 tagcttgtaa ttgtgggcgt tcgaccgatt gaccgcttaa tttgtctgaa aagaccttag
     2881 aaacgccctg taacgcttgt aattgccgat ctaagttctg ttctttgcta ctgacacgtg
     2941 cataaccaat tttagccatt ttcaaccaac ctctaaaatt ctctcggttg caataaccaa
     3001 tcagcaatat ctactttttc aatttcaaat tgcttatcag aaattgtctt ttcgtaagcg
     3061 ataaaatctt gcgcatattg ttgctcatta aaaatagcca ccacttcgtc attttctaaa
     3121 actcgataaa taaatttttt cattttactc ctcctattat gcccaactta aatgacctat
     3181 tcaccaagtc aattatactg ctaaaatcat attaggacaa ataggtatac tctaatgacc
     3241 tataaaagat agaaaattaa aaaatcaagt gttcgcttcg ctctcactgc ccctcgacgt
     3301 tttagtagcc tttccctcac ttcgttcagt ccaagccaac taaaagtttt cgggctactc
     3361 tctccttctc cccctaataa ttaattaaaa tcttactctg tatatttctg ctaatcattc
     3421 gctaaacagc aaagaaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg
     3481 ggttttattt tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gttttagggc
     3541 gttctgctag cttgtttaaa gtctcttgaa tgaatgtatg ctctaagtca aaagaatttg
     3601 tcagcgcctt tatatagctt tctttttctt ctttttttac tttaatgatc gatagcaaca
     3661 atgatttaac actagcaagt tgaatgccac catttcttcc tggtttaatc ttaaagaaaa
     3721 tttcctgatt cgccttcagt accttcagca atttatctaa tgtccgttca ggaatgccta
     3781 gcacttctct aatctctttt ttggtcgtca ctaaataagg cttgtataca tcgctttttt
     3841 cgctaatata agccattaaa tcttctttcc attctgacaa atgaacacgt tgacgttcgc
     3901 ttcttttttt cttgaattta aaccaccctt gacggacaaa taaatcttta ctggttaaat
     3961 cacttgatac ccaagctttg caaagaatgg taatgtattc cctattagcc ccttgatagt
     4021 tttctgaata ggcacttcta acaattttga ttacttcttt ttcttctaag ggttgatcta
     4081 atcgattatt aaactcaaac atattatatt cgcacgtttc gattgaatag cctgaactaa
     4141 agtaggctaa agagagggta aacatgacgt tattacgccc tattaaaccc ttttctcctg
     4201 aaaatttcgt ttcgtgcaat aagagattaa accagggttc atctacttgt tttttgcctt
     4261 ctgtaccgct taaaaccgtt agacttgaac gagtaaagcc cttattatct gtttgtttga
     4321 aagaccaatc ttgccattct ttgaaagaat aacggtaatt aggatcaaaa aattctacat
     4381 tgtccgttct tggtatgcga gcaataccaa aatgattaca cgttagatca actggcaaag
     4441 actttccaaa atattctcgg atattttgcg aaattatttt ggctgctttg acagatttaa
     4501 attctgattt tgaagtcaca tagactggcg tttctaaaac aaaatatgct tgataacctt
     4561 tatcagattt gataatcata gtaggcataa aacctaaatc aatagcggtt gttaaaatat
     4621 cgcttgctga aatagtttct tttgccgtgt gaatatcaaa atcaataaag aaggtattga
     4681 tttgtcttaa attgttttca gaatgtcctt tcgtgtatga acggttttcg tctgcatacg
     4741 ttccataacg ataaacgttg ggtgtccaat gtgtaaatgt atcttgattt tcttgaatcg
     4801 cttcctcgga agtcagaaca acaccacgac cgccaatcat gcttgatttt gagcgatacg
     4861 caaaaatagc ccctttgctt ttacctggct tggtagtgat tgagcgaatt ttactatttt
     4921 taaatttgta ctttaacaag ccgtcatgaa gcacagtttc tacaacaaaa gggatattca
     4981 ttcagctgtt ctcctttcct ataaatccta taaaataggt tgtttaatta acttggtttg
     5041 ctttttcatt caactgtttc aatattgcat gttttgaaaa agattttttt cctttataag
     5101 tcaatttttt tccactaatc gaataaatta ttttgttatt ttctattaac ttatatatat
     5161 aatcttcccc ctccgaagaa aaatacttat ctgattttgt ttctaagtag atatttctct
     5221 tttctaactc tttcttaaac gtttctagtg tatagatatt tgctaatttt cttatctcca
     5281 ataaactatt ttttatataa gttttacatt catcatgatt catacaaact ccaccttcta
     5341 taaatgaata caaaaaaagc aatcaaacga tttccgattg attgcttaac aattcttaaa
     5401 ttcagtagct tagatacttg aaaactctct gatttcccta tataatgata gtacggttat
     5461 ataccgtctt caaacaaagt taattaaata acttcttacg agggaagagt tcatctgact
     5521 aactgataag cgttggtttg gcaatcttat cgggctatgc atttataaaa tgtcgtcaaa
     5581 cattttataa atgtgtcatg gctctttttt cgtttctatt cagttcgttg tttcgttata
     5641 tctagtatac cgcttttaaa aaaaataagc aacgatttcg tgcattattc acacgaagtc
     5701 attgcttttt tcttcttcca tttctaaatc caatgttact tgttctgatt ctgtttctgg
     5761 ttctggttct gttggctcat ttgggattaa atccactact agcgttgagt tagttaactt
     5821 tgcaatttgt tctagtgttt ttatggttgg atctgatttt cctgattcta ttcgtgaata
     5881 atttgatcta ctcatttcta attcttggga gaatttttct tttgatttgg tacctctcat
     5941 cttttttaag ctttctctaa atgctagttc caagtttctc ccccctcttt ttgtgtaata
     6001 aaattcacgc tttatttaaa ttatagcttt taatatctta ataaacaagt gattaatcac
     6061 ttgtgatttg tgattattcc aaaagtaggt aatttcacca acctttttca aaagttttta
     6121 tcgacgatta ttggactgga tcgtgtttcc taaagcgatt ctaagcgcat gtttaatttt
     6181 aaagtgggta attctcttta aaacgtccta caaagattca aaaaaagcat ggctgctagt
     6241 gggggtaccg tcaggaaaat gcggatttac aacgttaagg aaatttatta aagagaccaa
     6301 ttcctcagca ctaaaattga taattgggct ttctttgtta ctatagtgtt aggtaagctt
     6361 tttactttat caccggtttt cccctcactt cacacctacg ggcgagtttc cccacatccc
     6421 gttttccatc taatccaatt cattcaatat ctttatgtgt atgaaagatc agattgcttg
     6481 atttagacat cttacacgta atttattctt aaatgggtac ggtaaacgag caataatacg
     6541 agcaaaagta tacctacaaa aataataact ctgtctaatt ttttttgatt catatgctta
     6601 ttgcttaaac catataattt cagtccaaga aagcctccaa cagtatttgt aattacatct
     6661 gttatgtctg tcgctccaat agcgaagata aattgaatta tttcaaaagt aagacttaaa
     6721 accagtacaa aagcaaactt aggtaaaaat ccgatttctt taaaattgac attcaaaagc
     6781 aagccaaatg gaataaagat tataacatta tctatcatct ctctgaaatt cccagtagca
     6841 gtaaatggag tcaagttaag acttctttga tgataattaa atactgataa aatattgtat
     6901 tgtaatttga ataacactaa ccagattagt gtcactaaat ataaagctag caatcctcta
     6961 gataatattt ttcccaatat aatttcctct aattcttttg tttaagttgt atcattagta
     7021 tagcaatgtc ctagttaacg tgggttttaa attaaaaagc taacgttacc ccaaaagtgc
     7081 tagtacaata atgctgttca ttaatttcgt tggtttcatt ccgccatcct tacctccttg
     7141 aattagtatg tgttgatccg ggaaacactg tcacaattac accgtctata ttgtttcctg
     7201 atatctcata acgaagatta gtcggcacat gaatggtggt atttttagta acaggataat
     7261 aaaagatctc atatttttcc ccatttacac taacagaaat ggttttttct tcttttaggt
     7321 aatccatata ttcctcgaga acgaaattct tttctttcat aatcgcacta tgtggtaaac
     7381 caacatagcg aatatgccat ggttcatatt gaattcctgt taactctgtt ttgtcctctg
     7441 gataacgtaa aatgaacccg tatttccaag cattttcttc tatccacttt ccttcagggg
     7501 ctcgttccat tttcgtcaag cttgatccta catctagtga taaacctgaa ttatgctcac
     7561 tataacctgc tggtaaggca tactcagccc ccatttcttg gtaaagcaca ctttgctcat
     7621 caaagtctcg atagccacta ttaataataa aatgactaac gccacccttt acagcatcat
     7681 tgaccatctc tgaaaatttt tgtgctattt cttttgacat ataaatatta ctatcaagca
     7741 acccgtatcc atttattaat tcgtcatgtt tagataaatt cacgatatct gacttcacac
     7801 tttcttggcg aacaggatat ttactattga ttaatagcag atttccttga taaacctgtt
     7861 cttctgtaat tgttttctct tgggtatttt cagaagtccc actattttct aaatgttctt
     7921 tgggattttg atcataattt tgaaattcga ctttttcctg agaaaacagt gcttcattaa
     7981 cgtagtcata acctaagtat attaagaata acaataacaa taaaaaaaac aacttcttca
     8041 ttttcagtct cctccttaac tatatagttg gaagtatgaa atatttctag aatcttcccc
     8101 ttaaacggca caatctcgtt tgagattgtg ccgttttgcc ccgtaaatta cagtcaatta
     8161 tttcgcccta ctggatttac tattatcacc aatgtagatg aattttcata agtgataccc
     8221 atgctgaatt taacgaggct gaactgcctg ttgacaatg
//
LOCUS       DAZKPO010000107         8248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000107 DAZKPO010000000
VERSION     DAZKPO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.110"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG8NZ_002621"
     CDS             234..1559
                     /locus_tag="ACG8NZ_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0540232.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG8NZ_002622"
     CDS             1552..1902
                     /locus_tag="ACG8NZ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540233.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACG8NZ_002623"
     CDS             1899..2069
                     /locus_tag="ACG8NZ_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540234.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8NZ_002624"
     CDS             complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8NZ_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0540235.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="ACG8NZ_002625"
     CDS             complement(3220..4173)
                     /locus_tag="ACG8NZ_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0540236.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="ACG8NZ_002626"
     CDS             4785..6278
                     /locus_tag="ACG8NZ_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0540237.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="ACG8NZ_002627"
     CDS             6413..6709
                     /locus_tag="ACG8NZ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0540238.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="ACG8NZ_002628"
     CDS             6810..7661
                     /locus_tag="ACG8NZ_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540239.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7894
                     /locus_tag="ACG8NZ_002629"
     CDS             7682..7894
                     /locus_tag="ACG8NZ_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540240.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DAZKPO010000108         7766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000108 DAZKPO010000000
VERSION     DAZKPO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.111"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..2446
                     /locus_tag="ACG8NZ_002630"
     CDS             119..2446
                     /locus_tag="ACG8NZ_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0540241.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2443..3357
                     /locus_tag="ACG8NZ_002631"
                     /pseudo
     CDS             2443..3357
                     /locus_tag="ACG8NZ_002631"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3430..3945
                     /locus_tag="ACG8NZ_002632"
     CDS             3430..3945
                     /locus_tag="ACG8NZ_002632"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0540242.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3942..4388
                     /locus_tag="ACG8NZ_002633"
     CDS             3942..4388
                     /locus_tag="ACG8NZ_002633"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0540243.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4538..5353
                     /locus_tag="ACG8NZ_002634"
     CDS             4538..5353
                     /locus_tag="ACG8NZ_002634"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0540244.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5559..6731
                     /locus_tag="ACG8NZ_002635"
     CDS             5559..6731
                     /locus_tag="ACG8NZ_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0540245.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6893..7387)
                     /locus_tag="ACG8NZ_002636"
     CDS             complement(6893..7387)
                     /locus_tag="ACG8NZ_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540246.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7384..7578)
                     /locus_tag="ACG8NZ_002637"
     CDS             complement(7384..7578)
                     /locus_tag="ACG8NZ_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540247.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2628 a         1457 c         1539 g         2142 t
ORIGIN      
        1 accttagaat aaaaagagaa aaaaagcccc aaactttcta tacatcaata aaataataaa
       61 aattaaattt agaataacgt gtaaaggatt atttgatgat tttttattga gaggagggat
      121 gaaaatgatt aagaaaactc aaaataaaat ccaacgtata tgtcaaaaac attttgtttc
      181 tagactcaaa gctgtatcta aaagaaaata cttacatccc attatgattt cgatcatctt
      241 gatttttact ttcttaggga gcttatcaag tatttatgta aacgtttata cagaaacgtc
      301 aaattttgct aaccaaacga cagaggagac aaatagtcca ccctattctg aaatacagtc
      361 catagaaagc accaatcagt ctagcaacaa cgagacaacc tcaaccacat cctcgactca
      421 actgattgat ccgacagaaa aagaaatcac agcagcaaaa gcagcagggg agaaagctta
      481 ttcagaaaca ggtagaagcc agacatttag tactgttgcg gccactgcga atttcaacgg
      541 acataccata ccagatggat atacagtgat atatgccaaa gatggagtca ttgttgcggt
      601 taacggatcg atgagtgcgg ataaaactgc gttacaaggc gtaaccaaag caatcgcttt
      661 tattgataac cgtccagata tccataatct gtttattggc tccacagtaa atggtactac
      721 tatcacattc ccaaaaagta tctatatggg ggtggctacc gctggaaata cgatcgattc
      781 agttacaccc aatgcggata aaagttattc ttttacaccc tcaacatggg gagggcttcc
      841 tgccggagga ggtatgagta ctttttcaat cggtatacag ttagcaaatg atattccatt
      901 cacggggtac tcgatcaaaa atgggcagat cgttgatggt aagggaaatg tttcaaattt
      961 tccaacctat caagcagcaa cggttcaggt caatgcgaat cggacaggaa ctttttctta
     1021 tacagtcaat ggtggagcag caaaaatcgg aacgtacggg actacattat ctggaatcca
     1081 tgtgggtgac aaagtggtta tcactccaac agcagtaacg ggctattctt ttgcacaaac
     1141 aaacggttct ttcacagccc tagaaagtaa cgtcgatacg gtgacctata caggcaaaca
     1201 aacaccaatc actattaagt atcaagacta ttttggacag atgattgctc cttctaaaat
     1261 aatgaatctt acttacggaa gtgctgcaca agacctgacg actaatacgc ctactatcaa
     1321 cggatatacc tttacagctg tttcagcgat agaaacaaag aaccaatcag caatgagtgt
     1381 tttagctagt ttagatacaa atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat
     1441 ttctgaagtc atcctatatt ataagacaaa tgcaacagta tcaatcaatg caaatggcag
     1501 taagtactat gatggtcttt ctgttgtacc gatcgttaaa tatgccttta atgataaaac
     1561 agaatcaaca tcccttttaa acaatgatct cagtattgat caaatcacat ggaatccaac
     1621 tgatttcaaa gcgatgaatg aaaagggagt tgaagtaact gcgcctactg aaattggaac
     1681 ctatacgtct tggcaattga caaaaagcgg actagctaaa ctagctgcca gaacaaacta
     1741 tttatttact atcgtgcaga catctgatgt ctatacgatt aagcaaatca gcggtgcggt
     1801 agagctcagt ggcagtaaga catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa
     1861 gctagcagag ggtgtcacta gttcgcttac tccagtacaa gtggcactta gcagtatgga
     1921 ttacacagca gatgcacaat cagcaaaaaa tatggttaat gctggcagct atacaattaa
     1981 actaacgaat agcggaatag acaaagtcaa agcagcaaat cccaagttgt catttactga
     2041 tatagccaac acatcaggaa cttataccat ctataaagct aacgcagtga ttactgtcga
     2101 tgacgctgcc tttaattatg atgcccaaag tcactcgatt tcggttggaa atctccacgt
     2161 taccggtgtt gtcttaggcg aatcattgga ttatacccta acagttaaca gccggacaga
     2221 tattggatca caaatagtaa cgattagttt aggcgaagga agtgtaaaca ataattatac
     2281 gattatcgca acagataccg ctaagttgac gatcaatccg actctcttgc ttccttcgac
     2341 aggtgggata ggtctactgc cctttgtctt tttaggactg atctttattt tttctggctt
     2401 cttttatttt atccatcgta agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat
     2461 gtacgtgtgg atatatttgc tgttctcttt tttatcctac tggtcttttt tataggttct
     2521 ccgactgtgt cagctagaga atcacctatt acatttactt tgcataatcg aatgttcgaa
     2581 agcgataaga tgccaagtat cagtcaaaac gacggaataa atatcccgga taactttcaa
     2641 ggagtaaaag ggttgaacgg cgttactttc gctatatatg atgtctcgga tgaattttac
     2701 aagctgcggt cagaaggaag tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt
     2761 gatagtgaaa aaatactggc agaaaatgtg acaaaaacag tagacgagga agagggcatt
     2821 gcttctttta gtgcatcgga caaagatgag caaggtcgtg acgctgttta ccgatttacg
     2881 gaaataaaga cttcagatta agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct
     2941 gtgacagatt catctaatca taaattgacg accatccact tgtatccaaa gagcgaacaa
     3001 aaaatacctg ctgcactcac attaacaaaa acagtagaga ataagcagac agattttgca
     3061 gatggggata aagtccctta tctcatcacc gtaacaattc ctgagaacat caacgagata
     3121 ggaacctata cgatcaaaga tacagctgat cctcagttat ggctagaacc agaaacgatc
     3181 gattttttga ttggtggaga caagattcat acatttcaca cacaaaaaat aaaacatggt
     3241 tttgtcttat cagattctgg aaaagatctt agttcgtttg ctggaaaaaa attaacgatc
     3301 cgttaccaaa tgacattgaa agaaaacagt gaaaaaacga catttgataa cgatgtacgt
     3361 ttgacgactg acaatcaaac agtagaaaca catcttgcca tccaaacagg aggcaaacaa
     3421 tttgtcaaag tggatcggca aacgaaacaa aaactagcag atgctcaatt tctagtcaaa
     3481 aaaaatgacc aatacttagc gcaagaaaac gggataaatc attgggtagc caaagatgat
     3541 tcaagagcaa cgatttttac ttccagtaaa gatggaacat tttctgtcca tggattgtct
     3601 tttggaagtt acgaactagt ggagattaaa ccgcctaaag gatatatatt gagtcaatca
     3661 accattctct ttgaagttga aaaaggcagt tatgatccgc ttcatcctat accattggaa
     3721 atcatcaacg aaccaaaagc aaatccgacg aataatggaa aaacgccagt tcagatgcag
     3781 acaaatggtc catcaaatga aaaaaatgga ggaagctttc caaaaacaag cgaagagatg
     3841 cttggaggtt tctcaatttt agggttattg ctagtccttt caacagggac agcttggttt
     3901 tataaaaaac aacaaaaagg aaacaatagg agggaaataa aatgaaaaac atcaaaacaa
     3961 aaatatggac gtttttagga actgctatca tgctgcttcc tttcgttctc ggcctaggaa
     4021 ctgcagaggt ttctgcagcc gtatcaccta caccagaaaa tgttacggtt aatttgcata
     4081 aattgaaatt cacttcagca ccagaaaatc aaataaacaa cggaacggaa ttgacatttc
     4141 cgaatagcga accgttgaac ggcgttgaat tcaacgtata tgacatcact gctacgtact
     4201 atccaagtaa agacacagca gttccagcag acgctacacc cttcgcaagt gtaacgactt
     4261 ctggtgaagg attagctaac ctgactcttc caggtaaatc tgatgggaaa gatgctgtat
     4321 acgtctttgt agaaacacca aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct
     4381 taccctagac cctgacaaac ctttagacac gattcaccta tatgctaaaa atgtcatcaa
     4441 agaaagcaat atcaaaatcc agaaagctgg gaacttttca ggggtagata agttagcagg
     4501 ggcgcaattc cagatcaaaa attcagagaa tcaattcgtg accggttttg atgaaaacac
     4561 tggtgtcgct ctctatgatc ctgctgagtc agcgttgaca ttcacaactg atactgaagg
     4621 atccatcagt gtcaatggat taatagacgg caattatact ttagttgaaa caaaagcacc
     4681 agatggttat caattaacca atccacaaaa agaaactact tttatagtaa aaaacggaaa
     4741 attgcaacaa acaaccggta tcatccatgg cgcagatgaa gccaatcttg ggaatccaaa
     4801 cggtgtagtc aatcgggaaa atacaactac ggataacacg attactgtag aaaatgtcca
     4861 aaactacggt gatttccact tcatcaaaca agatgtgaac acgcaagaaa aattagccga
     4921 tgcagaattt tctattctaa gagcaaaaga tccaactgtt aaattaatga aaaaaatagc
     4981 aaaccttgcc tcaggagaat acccatatgc ttggtcagat gaagtcacag gttctgactg
     5041 ggaagaagtg aaattgattt ctgattcaga agggaaattt tcaatcgaag ggctgaaaca
     5101 aggggcatac tgccttcaag aaacaaaagc accagaaggc tacgcattac ctaagaatga
     5161 agctgcgtac actgaattta cagtagatga tgatccaaca acttctgatg aaacaacgat
     5221 cgataaccaa cctaaaggaa tcttgccttc aactggtggt atggggatca tcgtattcat
     5281 cttggtaggt actgctttag ttggaggagc agtgatctat ttcaaaaaac gccatcaaga
     5341 aacagaagct taaactattg gaaacaaaaa tcatacgata aataaacgat agtgattcaa
     5401 atggggagaa aggttgtgac aagtggtcgg tctcttggta ttcgctcaac aaacggcaaa
     5461 ataggaggaa ctattttgcc gtttgcttcg ctaatactca ggtcaaggcg ctgatttcac
     5521 aacctcttca tatacatatt agaaaatagg tggacatgat gaataaaagc agaataagaa
     5581 tactggacat cttcatggca atcattttag tcgttgggat cgggatcttt tcttatcctt
     5641 ttgtagaaga ttcactgaat gattttcttg cccagcaaat gatcatccat tatcaaaagc
     5701 aagcatcgaa taagaattca gaggaaataa aaaaacagca ggaaaaaatg acaaagaaaa
     5761 accaacagtt ggcagagcaa aacgtatcac cagggatagg cagtttcaac caagcagttg
     5821 atgcaaaagc attaaaagat ttacctagta atgccttttt tatggaacat atgcttggag
     5881 tcatcgaaat cccaaaaatc aatgtcagct tacctatatt cgatcagaca acggaaatat
     5941 ttttgcaaaa aggaacgtca ttattggaag aaagttctta tccgactggc gggaaaaaca
     6001 cccatgccgt tctttctggg catcgtggac tcccagaagc aaaattattt acagatttgc
     6061 ctaagttgaa aaaaggagac cagtttttca tccagatcaa tggaaagaca ttggcgtatc
     6121 aagtagaaaa aatccaagtg gtcttacctg atgaagtaga ttctttgggt atccaaaaag
     6181 gcagagatct cgtgactttg cttacctgta ctccttatat ggtcaataca catcgactgc
     6241 ttgtgacggg gcatcgtatt ccttatcaag caaaagaggc aaaaaaagcg atccaaggaa
     6301 ttgatcaatg gaaaaaatgg aaatttttta tttggtttat cggtatacta cttggatcaa
     6361 tcggcttagt ttggctactc atcgcttatt tagattcttt ggccatcgct aagcgaaact
     6421 atcctttatc tttttatgta aagaacacaa acggacgacc aatcgaagga atggtctttt
     6481 ctgtaaaaac gctcaacggc aaacactata ttactagaga aaaagttcca tttgtgaaag
     6541 cttctgatga atacggacta gtgagatttt ctgatttaaa aggcaggaac tatcgcttgc
     6601 aacatgagga gctcctgctt aaaatccatg taaaacataa acattcgaaa cagttttcaa
     6661 tgaaattgaa aaaaggaaga tataaactgc gaaaagaaaa agaggtgtac tatttgattg
     6721 agaaagaatg aatgaagatg tccaaaaaag cagagggaac agactaaatg aaaaaagaga
     6781 gaagctgacg aaatggacgt gtaaactgtt atttttggtt tactatctgg aacgccatct
     6841 tctatttttt tatgtttcag aagaatttta ttattatagt ataacttttg aattagtttc
     6901 cataacattt ggagcaaggg gttaaaccac gagcttgagc ttcgcttaaa gtggcagggg
     6961 tatacgtacc atttccacat ttatgtgtat ggtattttga accggtcggt gtgacataga
     7021 cagtttgttc attattttgc tcaggagctg cggcttgtgc ctgctgagag gcctgttcag
     7081 ctgctgattg ctgtcgcgcc tgttctgctt gttgctgtgc gattgcttct tgcctcgcct
     7141 gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg ttctgcttgt tgtcgtgcaa
     7201 tttcttcttg ctttttccgt tcttcttctg cttcttttgc tttgattgtg gaatcaattg
     7261 cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag cgcttggata gctgctgaag
     7321 cagttgcata gttttctttt gtaggatttg cttcagcttg tgacaaagca gattccgctt
     7381 ctgtcacttt ttttctagtg cagctttttc cgctttttgt ttctcttctt ttttcttttc
     7441 aatttgttct tgttctttcg atgaatctgc cgtactttcg gagatcgtgg tactgctgct
     7501 ggaagcctga tctgaacttg tgctttgagt agtagacgta gtagcaccac acccactcaa
     7561 caaacttcct gcgaccaaaa acaatgcaac taattttttc gctaaatttc ctccttaagt
     7621 aaaaaacaac cgttttcaat aaatgtcctt ttcaattgtt tacttacttg ttataataat
     7681 tgtgttatga atagtgattg aggaaactta atctaatccc gtgttgatac acgggatttt
     7741 ttgctatagt tctgttatta agtatg
//
LOCUS       DAZKPO010000109         7724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000109 DAZKPO010000000
VERSION     DAZKPO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.112"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8NZ_002638"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8NZ_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0540248.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACG8NZ_002639"
     CDS             complement(2944..3378)
                     /locus_tag="ACG8NZ_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540249.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="ACG8NZ_002640"
     CDS             complement(3395..4600)
                     /locus_tag="ACG8NZ_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0540250.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="ACG8NZ_002641"
     CDS             5294..5680
                     /locus_tag="ACG8NZ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0540251.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="ACG8NZ_002642"
     CDS             5774..5965
                     /locus_tag="ACG8NZ_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540252.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="ACG8NZ_002643"
     CDS             6479..6811
                     /locus_tag="ACG8NZ_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0540253.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6721..6915
                     /locus_tag="ACG8NZ_002644"
     CDS             6721..6915
                     /locus_tag="ACG8NZ_002644"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0540254.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            6954..7100
                     /locus_tag="ACG8NZ_002645"
     CDS             6954..7100
                     /locus_tag="ACG8NZ_002645"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540255.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            7538..>7724
                     /locus_tag="ACG8NZ_002646"
     CDS             7538..>7724
                     /locus_tag="ACG8NZ_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0540256.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT         2566 a         1365 c         1121 g         2672 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaat
//
LOCUS       DAZKPO010000110         7663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000110 DAZKPO010000000
VERSION     DAZKPO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.113"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..767)
                     /locus_tag="ACG8NZ_002647"
     CDS             complement(<1..767)
                     /locus_tag="ACG8NZ_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0540257.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQL"
     gene            complement(887..1534)
                     /locus_tag="ACG8NZ_002648"
     CDS             complement(887..1534)
                     /locus_tag="ACG8NZ_002648"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0540258.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1618..3045)
                     /gene="uxaC"
                     /locus_tag="ACG8NZ_002649"
     CDS             complement(1618..3045)
                     /gene="uxaC"
                     /locus_tag="ACG8NZ_002649"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0540259.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3119..4198)
                     /gene="uxuA"
                     /locus_tag="ACG8NZ_002650"
     CDS             complement(3119..4198)
                     /gene="uxuA"
                     /locus_tag="ACG8NZ_002650"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0540260.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4201..5832)
                     /locus_tag="ACG8NZ_002651"
     CDS             complement(4201..5832)
                     /locus_tag="ACG8NZ_002651"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0540261.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6136..6807
                     /locus_tag="ACG8NZ_002652"
     CDS             6136..6807
                     /locus_tag="ACG8NZ_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0540262.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(6967..7158)
                     /locus_tag="ACG8NZ_002653"
                     /pseudo
     CDS             complement(6967..7158)
                     /locus_tag="ACG8NZ_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         2483 a         1498 c         1038 g         2644 t
ORIGIN      
        1 aataattgag caaggagtaa gaaaatagcc gcaccaatta tagaaagtaa tgccctattt
       61 ttaactaacc cttttaaaga ttttaacaat gaaactttgt attgagattg gtcaattctt
      121 actcgttcag tagttagttt ataacatatt aaataacaaa taagtgcaca tatcgaaaat
      181 attcccgcaa caattgtaaa agttgatcca ccacgaacta tttgagcgcc tgaatcatct
      241 cttgtataaa taaataatgg tgttaataca ccaatgacca aacttgctaa agtagctcca
      301 acatttcgga atgtcgatag cattgttctt tcttctgtaa tagaagtaat agctgatgcc
      361 attgatccat aaggaatatt tatagatgtg taaaaaatac tcccccataa tatatacgta
      421 acatacatgt atactacctt aacgctcata gaagctgaaa tcatagaagt ttgatacatt
      481 aaaaaacttg ctaaagcgac tggaccactc attcttaaaa tccacggttt aaacttaccc
      541 tgcttagttg ctttcgatct atcaacaata cgccccatag ttacatcagt aactgcatcg
      601 ataaatcttg ctactaaaaa taatgttcca acggcagctc cgcttatccc caatactttt
      661 gtataaaaga tcattaaaaa agaactagca aaaatgaaag taaaatcgtt tccaaaatct
      721 ccaaacatat atcctatttt gtctcttata ccaaattttc tttccataca attctctccc
      781 ttctgttgtt aagcgttttc attctgatga aagtacgtac tatactctga agcgtaaaga
      841 gtcagtattt attttttaca tttcagagta ttatttgttt tttctcctaa taattcttta
      901 tctcataaaa cttatctata tatttactag ctactcgact tacttcttca aaatcacctt
      961 tttcaacaga agccaataaa ttcccaccta caccaactgc aacaactcct acttcaaacc
     1021 aatcttctaa gttctctata cttactccgc cagtaggcat tatatttacg tgtggtaatg
     1081 gagcttgata ggcttttaca atggatggac caaaagcaga acctgggaat agtttaatga
     1141 tatctacgcc agcagtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat
     1201 aaggaatttg atataaatta caaatctcag ctgtttgtcg atcaaaagat ggactaacga
     1261 tatattctgc cccagccata attgcgatac gagcagtaaa cgcatctaaa accgtaccgg
     1321 ctccgattat tacatctttt tgatcagcat attttttagc aatttctttg attatttctt
     1381 cagcattagg aactgtaaaa gttagttcaa tccctttaat tccacccgca ataattgaat
     1441 ttattgcttt aatggcctca tccttatttt tcccacgtac aaccgcaatg actccagccg
     1501 cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcctt tatggataca
     1561 tactttgtct agctgtacta acttacaaag aataaattta agcttttatt tcaagtatta
     1621 ttttctactt ccgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt
     1681 ctccactatc tgactaagat acatttcatc atctggaatt cggcctgctt taacccactc
     1741 gcttagtaat tgacataata ctcgacgaaa atactcatgg cgaggatagc ttagaaaact
     1801 tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgtg ccatcgtagt
     1861 caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat
     1921 tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcagt
     1981 tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag cggtatcgaa
     2041 caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc
     2101 tactccaatt tgttccttta atatacttga attatttctt aaagcattta aatgcatttg
     2161 catcgtccaa ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa
     2221 ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt
     2281 gtcgagtatt ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa
     2341 tcgacagcca atttgatgaa aatgatccac tcgttggctc aatgcttgct tcaacccatt
     2401 aaagtctttg attggtatag ccgctgtttg actaagtttt aaaatatagt tcgaaaagcc
     2461 tgatgatttg atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt
     2521 atcctgttta gaaagctcag tatgatacca caagtcatct gtaggatcat ccgttgtaca
     2581 gatggctttc acatccatca tctccaacaa ttctttcggg cgataggaat ccgtttgaat
     2641 tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat
     2701 atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt
     2761 ctccactgtt tttacaaaag ctaaaaattt ttcataatca tcacctggac cactgatcaa
     2821 ctcttcttct ataccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt
     2881 atcatttaac catacctgag tcaaattctt gtattgctta ttttctagca aatatgctgg
     2941 gtctaaatga caatgatagt cgataattgg ttggtgtttt gcaaatttat gaaaaagtct
     3001 tttagctatt tcattctcta ataaaaagtt cttatctaaa aacatattta aacactcctt
     3061 tccttccgtt agtttttaga agagaaaatt tttcctctaa ataaactccc ttatataatt
     3121 atttactcgc ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag
     3181 ctcggtcata taatccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat
     3241 ctggacgaat atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat
     3301 ctagagaacc atattctgat ttatgcggcg cttcgtaaaa atcgagtcca ttgtcactta
     3361 aatatttgat attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata
     3421 gaatttcata gacatcatta tctggtcgtt ctgcaatact tccagtacat aatgtaatac
     3481 cattagaagg agaatccact acattacaca accaatctaa atcgtcacga tttttaacaa
     3541 tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg gtgaactgcc attttaatcc
     3601 caacttcttc acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgtaatt
     3661 tctcttcatc aacttcttta taggcttcta ataattgctt aatttgtgat aaccgatgag
     3721 gttcccaacc cggcaattca aaattattag tagaattttc tacttctcta ataatttcct
     3781 ccggatcagt agggatattt tttgaaatat aggctaacgt agaagaccca tcatctaaac
     3841 gataatccaa atccgatttt acccaatcaa atactggcat aaaattataa cagataacct
     3901 caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata attctcaatg tattgatctc
     3961 gtgttggtaa accaattttg atatcatcat gaacattaac actctcaata actttcaagg
     4021 taagtccatt ggcttcgact tcatctttta aagatttaat tcgttctctc ggccaaacct
     4081 ctccggctgg tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa
     4141 tttcttgtag tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag
     4201 ttactttcct ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc
     4261 tactagcatt tcgcaaaaga ttttttcagt tttctttcct accccaactt gatataaatc
     4321 taaaccaaag atttctttat tttctaaaat ttcctgtacc gcacaatgaa tctttgaagg
     4381 atcttgttca cctaatttca atactgataa tttatcctgc aactctacca ataacggatc
     4441 aggacttggc acaaaatttt ccccttgatc atcgactgcc agtaaataac gtaaccaagc
     4501 agcaatagcg ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt
     4561 tttaattgtt tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg
     4621 tggtgtatct ggaatatagg gattaggcaa acgtttatta attacttcat caataaattg
     4681 ttttggatca ataatgcctg gattttcaac tactggtaaa ccttctaaat aacctattcc
     4741 tttgattaaa ccaaccaaat catcatcctg catctctgct gaaattgatt gatatcctag
     4801 cagacaacct gttactgcca aagcagtatg aagtggattc aagcttgtcg taaccttcat
     4861 gatgtctgct ttatcaaccg tctctctatc tgtcataatc acaccagcct tttccaattt
     4921 aggccggcca tttggaaaac tatcttcaat aactaaataa tgaactacct cagtattagt
     4981 gaaagcagca atattggtat gtttttttgt gtgaatgata tctaaatcct cgagccccat
     5041 catcaataga tcttccttta cattctctga aggatttggc gtaattcgat caatcatact
     5101 ccaaggaaaa gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacata
     5161 tccttctttt tgccaagctt ctgcaatcgt aaaaatacta tcttgaaaac gttgaccatt
     5221 ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac tctccagcat taaatctgct
     5281 atataacaag gatgttacaa tactcatcgt atgttgagga ttgctaggtc cagatttgat
     5341 atcagctttg actaccgaaa agaattgccc tttagaatct tttaaactgt aacctttttc
     5401 agtaatagta aacgttgtaa actgtaattt aggatcttca aaatatttaa ccactctttg
     5461 atagtccgct atattcttcg gattgcaata taaactatca gccgttactg ccaaaagtct
     5521 ttttttgagt tcaccatctc cactcataac aatttccaaa atgtcattgt tatagtcatg
     5581 ataagctttt tccactactt cttcatcaaa tgtttcacac acaactactc ctgtttctaa
     5641 ttcattttga tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc
     5701 tccaccaaaa tgaagccata ctggatgttg ctggccttct tgcttaactt ctttgacatc
     5761 aaaacacggc agtagaatct gcatttcatt gaattcttcg atagaatttt gccaattttt
     5821 aatcgaaacc ataattaacc tcctcaaact gatatgttag ttttgataca ttttatattt
     5881 taaaacccat tgtaaccgtt gtcaatataa aaagggaata aacttgcttt tttctaccat
     5941 atcagaatga ttgtattttt tattcagaaa aataaaatac tccttctttt tggactgatt
     6001 ttatcgaaac ttattttttc tttttttcat gtattctttt atttttataa ctagtgtatt
     6061 acttcaaaat ttgatattat atggcatgaa cagaattaaa atatcagtta aaataaaagt
     6121 taaggagcat tgtgtatgac aattaaaact agtctccaaa atcaagctta ccaatctatt
     6181 cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa aagtatcaga aaaaaaatta
     6241 gaagaaacac tttctattgg tcgtacacca atacgtgaag cactgattca actccgacaa
     6301 caaggtcttg ttgatacaat tcctcaaagc ggaaattata tctcattaat tgatttagat
     6361 tcggccaaaa atgctcgatt tgtcagagaa catttggaac gacaaattat gttagagtgt
     6421 tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa ctataatttc agaacaagaa
     6481 aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata atgataattt attccataaa
     6541 gcttgttttg aaattgcaga gcgagaagaa gtatggcaat ggttagaaaa taacaataca
     6601 cacttagaac gatttcgatg gctacgagta aaaacggaag gattaaagtg ggaaaaaatt
     6661 atggaacaac attatcaact atttgaggca ttaacaaaac aagatccaga agaagctaac
     6721 tttctaacat cgttgcatct tcatttaatg cttaatgagc aaaatattgt tttagctact
     6781 catccaaaat atttcaaaaa tatataactt ttaactcttt ttaaattaaa tggttgttct
     6841 gatcagaaaa atatcgcttg ttttcttatt atgtaatata tttcataaac tattctgcat
     6901 aacaactatt gtttgtactt tttacattgg ataactaatt atgactgctg ataacttttg
     6961 atattaaact agtatctcca atacaaataa aaaatagata tcttgttcat caaaattagt
     7021 tcgtggatac ttaccttcat ttacatcatt cagcggtttc ccattttctt ttattctatt
     7081 atttcatttt ctaatagact tggcttcatt tcgcaatact ttttttcaat aggcatcaaa
     7141 ttggtgttgt ttagatattt agttgtagtt ttgtgacatg ataagtatgc tcctttttct
     7201 cttttccact aatacaacga tcaaagggtg cttttttcaa aaaactagaa aaatcaaaat
     7261 aagattcgta ttttgattct atatatcctg tttcaactac atcttatatt tcacaataga
     7321 ataaatagcc ttttgcatag ctgcttaact tataagaaat tgcaatttac ttcttcaaag
     7381 tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca gataattaaa aatagcgcgt
     7441 aacaaaaatc ggcattcaat cacataatta ggattgatgc cgattactta tattctacgt
     7501 atagttttag tattcttatt ttttatcacc agtaaccaat tgagaacagc cctaaagcag
     7561 aatagtcttt tatctcaaaa cgcttaattt caggcttagg aatatgaata atcttttgag
     7621 tatattcttt ctcttttaat cttactgaag aaataccact gcc
//
LOCUS       DAZKPO010000111         7599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000111 DAZKPO010000000
VERSION     DAZKPO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.114"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG8NZ_002654"
     CDS             complement(136..426)
                     /locus_tag="ACG8NZ_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540263.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG8NZ_002655"
     CDS             complement(544..804)
                     /locus_tag="ACG8NZ_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540264.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACG8NZ_002656"
     CDS             complement(941..1354)
                     /locus_tag="ACG8NZ_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0540265.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACG8NZ_002657"
     CDS             complement(1810..2295)
                     /locus_tag="ACG8NZ_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540266.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACG8NZ_002658"
     CDS             complement(2434..2649)
                     /locus_tag="ACG8NZ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0540267.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACG8NZ_002659"
     CDS             2873..3673
                     /locus_tag="ACG8NZ_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0540268.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACG8NZ_002660"
     CDS             3692..5560
                     /locus_tag="ACG8NZ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0540269.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACG8NZ_002661"
     CDS             5553..6044
                     /locus_tag="ACG8NZ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0540270.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACG8NZ_002662"
     CDS             6032..6586
                     /locus_tag="ACG8NZ_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0540271.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACG8NZ_002663"
     CDS             6604..7599
                     /locus_tag="ACG8NZ_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0540272.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKPO010000112         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000112 DAZKPO010000000
VERSION     DAZKPO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.115"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8NZ_002664"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8NZ_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0540273.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8NZ_002665"
     CDS             1483..1935
                     /locus_tag="ACG8NZ_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540274.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8NZ_002666"
     CDS             1949..2638
                     /locus_tag="ACG8NZ_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540275.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8NZ_002667"
     CDS             2763..5114
                     /locus_tag="ACG8NZ_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0540276.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8NZ_002668"
     CDS             5194..5589
                     /locus_tag="ACG8NZ_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540277.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8NZ_002669"
     CDS             5914..6531
                     /locus_tag="ACG8NZ_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540278.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8NZ_002670"
     CDS             6572..6871
                     /locus_tag="ACG8NZ_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0540279.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8NZ_002671"
     CDS             6905..7072
                     /locus_tag="ACG8NZ_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0540280.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8NZ_002672"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8NZ_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540281.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKPO010000113         7212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000113 DAZKPO010000000
VERSION     DAZKPO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.116"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8NZ_002673"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG8NZ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0540282.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8NZ_002674"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG8NZ_002674"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0540283.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8NZ_002675"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG8NZ_002675"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0540284.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8NZ_002676"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG8NZ_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0540285.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8NZ_002677"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG8NZ_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0540286.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG8NZ_002678"
     CDS             complement(4433..5290)
                     /locus_tag="ACG8NZ_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0540287.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8NZ_002679"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG8NZ_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0540288.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2092 a         1589 c         1255 g         2276 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt ta
//
LOCUS       DAZKPO010000114         6844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000114 DAZKPO010000000
VERSION     DAZKPO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.117"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            528..722
                     /locus_tag="ACG8NZ_002680"
     CDS             528..722
                     /locus_tag="ACG8NZ_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540289.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            913..1162
                     /locus_tag="ACG8NZ_002681"
                     /pseudo
     CDS             913..1162
                     /locus_tag="ACG8NZ_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1406..2236)
                     /locus_tag="ACG8NZ_002682"
     CDS             complement(1406..2236)
                     /locus_tag="ACG8NZ_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0540290.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2444..3778
                     /locus_tag="ACG8NZ_002683"
     CDS             2444..3778
                     /locus_tag="ACG8NZ_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0540291.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3819..4730
                     /locus_tag="ACG8NZ_002684"
     CDS             3819..4730
                     /locus_tag="ACG8NZ_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0540292.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4792..5580
                     /locus_tag="ACG8NZ_002685"
     CDS             4792..5580
                     /locus_tag="ACG8NZ_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0540293.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5577..6193
                     /locus_tag="ACG8NZ_002686"
                     /pseudo
     CDS             5577..6193
                     /locus_tag="ACG8NZ_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6195..6713
                     /locus_tag="ACG8NZ_002687"
     CDS             6195..6713
                     /locus_tag="ACG8NZ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0540294.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2058 a         1186 c         1373 g         2227 t
ORIGIN      
        1 gttattttat aataaagata tgatgactaa tttagctcaa ctattccagc gtcagctgaa
       61 gatattgcaa ttcccgattc cttaatcatt tatctggatg agacagaaag tgaagttgca
      121 acagatgtgc atgtagttga ggataaaaat gatagtttaa aaattaatag ttcagatact
      181 ttactaagag taagtcctat cttttcagct gttatttaga tgcaagtagc aactaagtta
      241 ggtagtactc ctgcaatgga taggtatttg tgtgttcgat atataagtgg tcgaagtatc
      301 aaggttatgt atactggatg ggagatagaa gagttaaaaa ttggaggtca ccgccacaaa
      361 tggaatttag atttagaggt accttagatt tggtatagtt attaaacaca agatggcctg
      421 gtttgtaggg aaagtcgaag agtatttttc cacttctgcg cccctttaat ttttctagta
      481 atgccgtgaa tttgtgatta attggaaata tgaagaaagg agagtctatg aaaacaacac
      541 tagttctatt ctataaaaag catccttatt ttacacttct gataaatata ttgttggcta
      601 gtgttatcgg gatttctgtt gaatatctaa taaataaaga tttcataggt tcttgttttt
      661 atacggcttt atttcttgga cttcttgaag cattttccat ttataaaaaa tcaaaaaaat
      721 aaaatagatt gccgtaagag atctttttca tttcattttt ttctgaaaac ccgttgtttc
      781 tgtcagtgaa gcagcgggtt tttagattta ttgtctttag acctaattga gcgcgccgag
      841 cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc
      901 cgtacaaagt agatgtgtaa ataccacatt gtattcacac caaagtatag aagaaaaata
      961 atttataatc aatatcgagc caatattcag gagatcatca agagattgtg caaatctaaa
     1021 atataaattt gggtatcgac acttttgggt ggaaggatac tatgtgacaa aagtaggaca
     1081 aaatgaagcg accatcaaaa agtacattca agaacaagaa aaacatgata gtgcgttaga
     1141 taagctgagt gtacgagagt aagacgatcc ttttaagggc taaggagtag tacataaacc
     1201 ttttaagagg tgggcaaaac tggcaaaggc aatatggctt gaacataaaa tatgaaagcc
     1261 agcgtcttta ggcgctggcc ggtgatttga gcttatagct ctagagaaaa ccaccctttt
     1321 gaagggtggt tatgattaat gactttagtc agttttgctc acaaagcgtg tggaacaaaa
     1381 aacgggtgtt tctacgggta gatatttatt tcttgaatgg aggtgtccca tacatttttg
     1441 gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt aggttctttc aaccattgat
     1501 attcgccttc gccatccagt gctttgccac tagcccaaga accttcacta ctttggtctc
     1561 cctctgagaa gttatagaag tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag
     1621 tacttgggac taagattgaa ccttcttttt cttctaattc tttcactgca cattcccata
     1681 gattttgatg atagctatct cttgcaagga gaacagacag catatctttt acgccacgat
     1741 catcgatcat ttcgtaaata cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat
     1801 tagctctaaa gtctgctaac aagttgccac tcgcaatgat ataggcaccg ctccatggat
     1861 tgcccacgct gtcagcagga cgaggtccta agccagctac gatcgcgtgc tgtggattca
     1921 ttccagagat aatggcaccg attgttggat ctttttgcat cgctgcttct tgatcttcaa
     1981 caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa catttccacg tggccgattt
     2041 cttctgttgc aatgtccatc agcatatctt tatatttttc ttctcctcgg cagctccagc
     2101 cttggaacag gtactgcata gctacactca tctcgccata ttggccgccg atcagctcct
     2161 gcagatattt cgcgattaat ggattcggac gatctggttt tgcttcaaac tgcaattctt
     2221 tctggtgtct aaacatcaaa attcctccta atctattttt aatacattct cattctgcta
     2281 cgaaagatta aaaaattcaa agaaagcgct cgcgaattat ataaagtatt ggtatcatta
     2341 ataatttttt tagaatcata ccttataaat tgttattttc agaacaaaat gatatcgtta
     2401 tattaaaacc ggcatcggtt acattaaaat ttggaggtaa aatatgaaaa aattattgtt
     2461 tacagggttg ctggcaagtt cagcattgtt tttattagga ggatgcggat caagtaatgc
     2521 aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa acaagtgatg gaagaaaaaa
     2581 tattgatttc tggtcctttt ggggatccgg tgcacgaaaa gaagctatcg aagaaatcat
     2641 tgaggatttc aatcaatcac aaaacgagat ccatgtaaat tataagtatc agccatgggg
     2701 tgacatctgg actaaatctt tatctgcaat aacagctgga aatgcacccg atgttattgt
     2761 acaggacata aattccgttg cccaaagagc agaagcacag caagcaacta atctctccaa
     2821 atatgtagaa aaagacactt ctaaggattt ttatccgcag ctttgggata cagtagaata
     2881 tgaaggtgat ccttatgcga tcccattcaa tacagataca caagtcattt tttataataa
     2941 aaagatattt aaagaagctg gaatcagtga acaagatcta ccgacaactt gggatgaatt
     3001 agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa gaatttacac gcatcggttt
     3061 ttatccatta tggaatttag gggctgatgt atgggcgcta aacgcagata acgggactag
     3121 ttggtttgac aaagatgaaa aagtcaagat tgatactaaa aataaagtgg aagccttgga
     3181 gtggatccaa gactggcaag attattatgg gaaaaatacg atcaataaat tagaagccga
     3241 atttgggtct ggtgtatctg atccatttat ttccggatta gtagccatgc gtgcccaaaa
     3301 tatcaattat tactctagtt tgatggaaaa tgcaccggat gattttgatt ttggcgtgat
     3361 gcagctccca gaaaaagaag aaggttcagg ccattggtcg tggggtggcg gattcgtctt
     3421 ggaagtacca tacggagcaa aagatcctga ggcctcttat gaattcatca aatatctttc
     3481 ttctccagaa gtacaagaaa aattcggcga aaaaagtttt gatatcatgg cgagcaagac
     3541 agccaatgag aatttagtga ataatgacaa cctaacggat aaaggaaaaa tgatctatca
     3601 aatggcggac gaaaacttta aaaatacggt gatcacacca gttcctttag atgctccgga
     3661 ttacacgaca ttagtgaatg aacagattga ccagattctt ttaggaacaa aatcaccaaa
     3721 agaaggattg gcagatgcac aaaaagcagt agaaaactta gtcaagcaaa atcaataatc
     3781 catttttgac tgtctaggta cagaggaggc aagaaaattt gaagcgttta tctatccgaa
     3841 agaaaaaaga agcaagatgg gcctatttat ttatctcgcc ttttattatc gggttcttgg
     3901 tatttatgct agggcctatg cttttttcag ttattggtag tttcaccgat tataatttga
     3961 catccaagat gaatttcatt ggtctagcaa atttcaaaag aatgttcgtt cacgatgatt
     4021 tattttggaa atcgttatac aacaccgttt atttcgtcat cttcaatgta ccgctgacga
     4081 cgattttcag tgtgttttta gccacagtac tcaatcaaaa aatcaaagga atcagtgtat
     4141 tccgtacagc tttctattta ccggcagtct tatcaggagt agcagtctac atcttatgga
     4201 tgcagctgct ttctccatct gcaggattga tcaacacatt tctgggctgg ttccatatcc
     4261 ctggaccagc ttggctgttt gatccacaat ggacgaaacc tgcgttgatt ttgatgaaaa
     4321 tatggagttc tggaggagca atgctacttt acttagctac attacaaaat gttcctagat
     4381 ctttatatga atcagcagaa atcgatggag caggcgtttg gggaaagttc aaaagtatta
     4441 ctttgccatt aatcactcct attatttttt acgatgtgat cacctctacg atcggttctt
     4501 tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg aacaggcgga cctgcaaatt
     4561 ctttgctttt ttataatctg catatgtgga acaaggcgtt cgtagctttt gatatgggtt
     4621 atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt ggttctgacg tttgtcaatt
     4681 tgaagcttgc acctaagtgg gtctattatt ctggaggtga cggcaaatga tggattattt
     4741 caaaaagacc agtgtgaatc ggcaaacaaa aaaatactaa gttatgtcct catgacagta
     4801 ataggaatca ttttgatcat tccattgctt tggatggtgt ttacttcatt gaaaccaatg
     4861 gaagaaatcg ttcgttatcc tccgaccttt ttcccagaaa aaatcgtatg ggaaaactat
     4921 ctggatacga ttgccgcctt tcctttttgg cgctatgcaa gaaatacatt attcatcact
     4981 gtgctagtag tgatcggtaa tgtcctttca aattctttta ttgcttatgg atttgcgaag
     5041 cttgattttc ctgggaaaaa gctgatgttt gctttggttc tttctacgat gatgatcccg
     5101 gggtttgtaa cgatgatccc tcaatatgta ttattctcca agatcggctg ggtaggtact
     5161 tatttgcccc tgatcgttcc atcgttcttc ggcaatgcgt tcaatatatt tctgatgcgt
     5221 caattttact tatcgatcaa taatgaattg atcgaagctg ctgaaatcga cggagcgaat
     5281 catttgtata tttggagtca tttgatgctt ccattgacga aacctgcctt gattaccatt
     5341 gcgatcaatt ccttcaacgc agcttggaat gatttcttag gtcctctgtt atatatccaa
     5401 gatcaagaaa aatacacgtt acagattggc ttgcaagttt ttcagaacca agcaacgaca
     5461 caatggaatt atctgatggc aggagcaaca cttgtcttga tcccgacgat tttgctgttc
     5521 ttttttgcac aacgttattt cattgaaggg atggatttga caggaggttc aaaaggatga
     5581 tgaatcgatc gattggtaaa aaagccaatg attatttctt agaaatcatt gaatggattc
     5641 cacgtcttat ccatttacag ctactatggt tcctgtgcgt cttgcctgtt ttcacattag
     5701 gaactgcaag tcgaacagtt ctgtatatga tttatcatta caaaaaaatg aagagaaaaa
     5761 gctcggttca ctattctgga aaaagtttcg tgagaacttc tctctttatg gaaagcaaga
     5821 tagtttagca agtgtttatt ttcttcttct attgatcgat tatcagattt tccgctattg
     5881 gggtggggga attggctata cattgatgta tactacgttg tttttgcttc tattaagcgc
     5941 gcttctcttt agttacaaaa tcttgcttca actggaacaa aaagaagctt cttggattac
     6001 tgcatttttc ttgtttttta atgatttcaa aagcgcgctt ttctatttgg caggcacgtt
     6061 gatccttctt gtcgcattct ggtttgcagg accaatctat tttgctttat taggtttcag
     6121 tttcttgttt tattttcaag tcctgctttt tatccatcgt acgcaagaaa aggttcttaa
     6181 aaaggaggaa taacatgttg cgtttgattg ctaaaatcga tgatagtatt atcaacaagt
     6241 tcgccaagca acggaaaacg gatagtcgaa aaatcaattt tttgaaattt atcagtaata
     6301 tctctaatgg tggttttatt tggttgctcc ttgctgctgt tttagtcttg atacctgata
     6361 ctagagaata tggtctagcc attcttatcg gcagtttgat ctattttttt gttggtttgc
     6421 tttttatcaa aaaaacgaca agacggaaaa gaccttatga aaaaaatcgt cggcttgtcc
     6481 tttacacaag taaaccgaag acttcttctt ttccttcagg acatacagcg ctagcctttt
     6541 tatcagtagc tgcgttgttc gaattaagtc cagttgcagg aatattagcc tttttatttg
     6601 cttgtttgat cgcttattcg aggatttttt tacttttaca ttatcctact gacattttag
     6661 gaggagctat tttaggaagt gttattggca tcctatgcgc tgccgttatc tgaattaatt
     6721 gtacgaataa agcatgagtg cgtaaatgcg catttgtgct ttattttttt gtttcgagga
     6781 aagcgtagtt gtttattcat ttgtcttaat aacttaagtt ttgttaaatg agaacgttat
     6841 aaaa
//
LOCUS       DAZKPO010000115         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000115 DAZKPO010000000
VERSION     DAZKPO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.118"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8NZ_002688"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8NZ_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8NZ_002689"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8NZ_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0540295.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8NZ_002690"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8NZ_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8NZ_002691"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8NZ_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0540296.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8NZ_002692"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8NZ_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0540297.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8NZ_002693"
     CDS             3582..4271
                     /locus_tag="ACG8NZ_002693"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0540298.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8NZ_002694"
     CDS             4285..5787
                     /locus_tag="ACG8NZ_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0540299.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8NZ_002695"
     CDS             5800..6276
                     /locus_tag="ACG8NZ_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0540300.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8NZ_002696"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8NZ_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKPO010000116         6479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000116 DAZKPO010000000
VERSION     DAZKPO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.119"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..939
                     /locus_tag="ACG8NZ_002697"
     CDS             349..939
                     /locus_tag="ACG8NZ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0540301.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            966..2276
                     /locus_tag="ACG8NZ_002698"
     CDS             966..2276
                     /locus_tag="ACG8NZ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0540302.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2273..2992
                     /locus_tag="ACG8NZ_002699"
     CDS             2273..2992
                     /locus_tag="ACG8NZ_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0540303.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3007..4092
                     /locus_tag="ACG8NZ_002700"
     CDS             3007..4092
                     /locus_tag="ACG8NZ_002700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0540304.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4228..5760
                     /locus_tag="ACG8NZ_002701"
     CDS             4228..5760
                     /locus_tag="ACG8NZ_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0540305.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            5938..6159
                     /locus_tag="ACG8NZ_002702"
     CDS             5938..6159
                     /locus_tag="ACG8NZ_002702"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0540306.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2267 a          693 c         1256 g         2263 t
ORIGIN      
        1 aagcggaatg ggtagaaata ggagatttta atcataaaaa actgtactat agtgaatttt
       61 tggagattta tattccagtt atatattaat gattgtgata cttgtactat tgtaaaaatt
      121 cgttaggttt aaataactgg aaatatcgga aaatgggcat tatacatcac ggacatccct
      181 tcttttggaa aaaatgttac gatttttata agtaatattg tatccggtgg aatcaacgat
      241 gaagataaca tatccatcgt tgtagattca gtagcattga atgatgctct tgttgcaaga
      301 aatccgaata aaattgttaa gtataaattg aattaatagg agatatgaat ggaaattagc
      361 agtaaagaat atttaaaagc ggttcttcgt atggaaaaga atctatatat agaatctaaa
      421 ggacaacaca caaaattact aaaaataatg aaatcacatc cggactacaa cgcttggcgg
      481 tatactcgtc gaatgagaat tacatcttat tattattcaa agagaaagaa aaatatcatt
      541 tatgcgatta tgtatattgt gtcttgcaga agaatgaata gtttgggaag aaaatgtggt
      601 atagagtgtg gtgaaaatgt atttgatgaa ggtgtaagat tttaccatac acaaggaatt
      661 gttattaatg gtaacgccat aataggaaaa aattgttact tatatgggaa taactgtatt
      721 ggaaatgatg gaatcgatcc taagtgtcct gtaattggaa ataatgtacg tgtatgcgtg
      781 ggtgcaaaga ttattggtaa tgtaaaaata gctaacaatg tagttgttgc ggcaggagct
      841 attgttatca aagattgtct agagaatggt gcaattcttg caggtgtacc ggcaaaaata
      901 atagggtatg ttaatttaac aaaagtattt ttagaataat ttttagcgaa agaaggtgaa
      961 taacgatgcg tatttcaata aaaagatggt caggagcaga ttggacattg ttcacgttgg
     1021 cattttttgc aatctccata gttcttgtat atacttttaa cgccccaaga gcaattttat
     1081 atttgggaga tataattaat atctttgtgt tattaaatgc acttagcttt agacgcagaa
     1141 aaggaatata tgctagcaaa aatgagatgc tgatagtttt gtttttacta gtttctattt
     1201 gtagctcagt tattaattta gaaaatatag gattgattat ctgggggata cgaaataaca
     1261 ctcgtatgtt catagtatat ataaattgtg caacatattt aacaattaaa gattactata
     1321 aagtattcgc tataattgaa aaattattct ggatttcact gccactttgt ttaattgaaa
     1381 gattcatggt cacctatcca actggaacaa ttgtaggaga tatgataggt ggagtttttt
     1441 ggaattattc aggttgtaat atgccattga atttaatttt gtgtgtgaca gttactgttg
     1501 ttacaatcgg atatttcaag ggagaaaaaa atttcatcca ttttatttta tgtgtttgtg
     1561 cagcactttt tatgtctgca ttagctgaat tgaaagtttt tattcctgaa ttatttataa
     1621 tatttgttgc aatagtattc actttaaaag ttgattttaa agtagtgatc aagatattat
     1681 catgcgtaat acttgtgtcg atagtattat ccttttttat aacaatattt attacattga
     1741 acgataatgg acaaagctat gctgataatt tcacaattca aggtctttta gaatatgcgt
     1801 caagagattc tggatatgat ggtgttggtg atttaaatcg aataactggt atctctagag
     1861 tagtagatga aatatttaat aataatttta gattagtcct aatgggagta ggcattggca
     1921 atgcagagtt taccaatttt tttgtcagtg agttttataa atcgtttggt aatcttcatt
     1981 atcagtattt tcatcatatt tggttattta tagaagttgg aatattagga gtttcgatat
     2041 tcctattttt tataataaag acatttttca atgcaaaaag atcttttatg aaggataaag
     2101 atgcaagtct tgttaaagtt attgcaatta tgtcggtatt cttttttgtg tataacacaa
     2161 cacttagggg tgaaacaacg gggccactga tttatatgat tttagccatg cctgaagttg
     2221 tgaaaaggaa agagcattat tatagtgaaa aaaatacaaa aggagagata ttatgattcc
     2281 taagaagata cattactgtt ggttcggggg aaatccactt ccgaaaaaag atagaaagtg
     2341 catagaaagc tggaaaaagt attgccctga ttatgagatt attgaatgga atgaatcaaa
     2401 ttatgattta aataagaacc gttatatggc cgatgcatat aaagaacgta aatggggttt
     2461 tgttccagat tatgcaagat ttgatattat ttataatcat ggtggatttt atttagatac
     2521 agatgttgag ctaattaagt cgttagatat acttcgtgaa aatcatgcct atatgggatt
     2581 tgaaggggaa atctgggtta atggcggtat tggttttggt gcagaagaag gacattctgt
     2641 tattaaaggt ttaagggata tttacgattc attaagtttt tatcaaaatg atggtacatt
     2701 gaacttgaaa ccgagtccgt attatattac agattatctt gaatctattg gattggttcg
     2761 cgataacagc gaacaaataa taaatggcct gcatatctat cctacggatt attttgctgc
     2821 caaggattat gatactggaa aaatttataa aactagtaat acaataagca ttcatcaata
     2881 taatgcatct tggaaaacac ctaaacaaaa atttgtttat agattaaaat caacaattgg
     2941 acaaaaaaaa tttaacaggt tacttgcact gaaaaaattt ttacacttat aaaggagaaa
     3001 agtactttga agaaaatttg tttcattagt ggtgtaattt ctcgttctgg gggaactgaa
     3061 agagtaggga gtattattgc caatagcttg tctgatttag gttatgatgt cactatttta
     3121 agcttttgga atagtggaga gccttatttt gggttagata agacgattaa ggttgattat
     3181 atgttaaatc ctaaaacgga gggaaaatta tatagaactt atttatatcc ggtttggaaa
     3241 ttgcacaagt atatatgcaa aaatgaattt gatatagtaa ttgatattga tactttgttg
     3301 tctttgtata ctacaagggc aattaaagga acaaagtgca aattgatttc ttgggaacat
     3361 tttaattact ggacaatgga aatgctgaaa gaaaagaaga gattcaaggc taaaaaattg
     3421 ataaaaaaat atgcaaagag gcttgtggtt ctcacagaag aagatcgtca aaaacatatt
     3481 gatgaatata gtttatcaga agattttgtt ataactatgc caaatccgtg tatatctaat
     3541 atcgagtacg aatatcatta tgataaaaag agatttcttg cagtagggcg attagtggaa
     3601 gagaaaaatt ttgctgacat gataagtgct tggaaaattg tagaaaaaaa agtagatgat
     3661 tgggatctta tcattgtagg aaaaggtgaa ttagaaaaag atcttcgcca gcagataaaa
     3721 gatttaaatc tgcaaagagt aactatagca gggcattcag accatgtgga ggaatattat
     3781 aaatctgcat cttgttatgt gcttagttct gtttatgaag gatttcctat ggttatatta
     3841 gaagcacagt cttatggatt accagttatt tcttatgatt gtaaaactgg tccgagagat
     3901 ttggtacatc ataattttaa tgggatgttg gttgaagata agaatataga ccaattagct
     3961 aagagcatga taatgtttac taagaatact gatttagcaa tgagaatgtc actgaacgca
     4021 tataataatg tacaaaaatt taatttgaaa gaaattacaa aacaatgggt tgctttgatt
     4081 gaaaatgtat agttttttgt ggttgtccaa acatgaaaat tagcgtaatt aatcaaagag
     4141 ctagaagaaa cctattacat ttcaagattt tagaaattat atagtattag gtaacaataa
     4201 aaaatagaaa gtttggagcc atatgatatg ataattaatc gtaaacaaaa tgctaaaagg
     4261 aatataatta ctggaacaat ttttaagtta tatcagatta tcattccctt ttttatgcgt
     4321 acacttatga tttatcagat gggaatggag tatgctggat tgaacaacct gttcacatct
     4381 atatttcaaa tgttaaattt agcggaattg ggaataggtg ctgcactaac tgtaagtatg
     4441 tataaaccta tagcagaaga caatagaaaa gagatttgtg ctttactgaa cgcatataaa
     4501 aagtactttt caattattgg atggatagtg tttacattag gattgttatg cattcctttt
     4561 ttgagatttt tagttaaagg ttcgattcct ggaaatttaa atatgacagt tttgtattta
     4621 atgtatttga taaatactgt tttgtcgtat tggttatttt catatagaaa gagtctaata
     4681 tatgcacatc aaagaaatga tgtaattaac gtgtctatgc taattacttt tacattccaa
     4741 tttgtaatgc aggcatatgt tctgattttc ttaaaaaatt actatttgta tttattggct
     4801 tctattttgg gacaaatagt tcttaactta atgtgtgctg ttgtttctca aaagatgtat
     4861 cctgattata ctcctgttgg tgagttagag aaagaaacta ttgacgatat tataaaaaaa
     4921 gtaaaaggat tagttacaaa caaggtaggc ggtacaatat tacgatcttc agattcaatt
     4981 attatttcat ctttcttagg tctttcagta cttgcgattt atcagaatta ttactttatt
     5041 atttctgcca taatgagtat atttgcaata atttatgaat catgtatagc aggtataggt
     5101 aacagtctta ttgtagaaag tcaagaaaaa aattatatag atttttgtga tattacaaca
     5161 attataggtt ggtttctggc aattagttgt gcatgcttta ttacattatt tcagccgttc
     5221 atgattatct gggtgggaag tgataatctt atgagtattt catttgttat atttatgata
     5281 gttcattttt tcttatatga aattgatcag ttaattgggt tatacaaaga tgctgctgga
     5341 atatggtacg cagacaggtt tagaccatta attacagcaa tgataaacat tgtattaaat
     5401 atcatcctcg ttaattggtt cggattatat ggagttttgg cggcaaccat tgtttcagta
     5461 ttgttggttg atttaccctg gttattatat aatgttttcc aaaatatatt taaatttgaa
     5521 tcggcaggta aatatgtttg gaagctcata agtttgtgtg taatagcttt tgttgcttct
     5581 tatttaagtt attttgcttg ttcgtatgtt aatatcggaa atttcaaagt gataagttta
     5641 gtgataaatt tagttatatc ggttgtcata tcgattttag ttattcttct gtttaatatc
     5701 cgtaataaga agtttaagta tattttgaaa gttcttacgt cggtattggt aaaaagatga
     5761 gaagagacat cagaatgata tataaagtaa ctacaatttt taggctgccg aataggcgaa
     5821 cgatgtatag tttttgttcc aacgaagact cttgttgaaa ttggagatga tatgaaggag
     5881 ttacaatttt gactcatgtt tatgattgat cggttttaaa agtagtatgg agaagctttg
     5941 ggatcatcgg agaggatcaa aataggtaat aatgttttta taggaatgaa gtctagaata
     6001 ctaaagggtg tacatatagg aaataatgta gttataggtg caaatagttt agtaaacaag
     6061 gacgtatcag acgattgtgt tgttgcagga aatctagcaa aagtaattat gtccctagat
     6121 gaatattata aaaagataaa caatatgaag aatccgtaaa acttgtgaaa ctataccgag
     6181 aataatatgg gaaaaatatt gatgaaaaag cattatatga gtttttaggg ctgtttacag
     6241 atggagatga caatttgtcg aagagttggg agcaatgcaa aatttagtag gggattataa
     6301 acagactgac aaggtattta aagaacatgc acaagtattt cataatattg aagaattttt
     6361 gaatactgtg agataggagt tgctacattg agaaaagtat gtatttttcc aatgtaaaag
     6421 attacgagtt tgaaaacgac accataatat tattttttaa gaagatatta tagagctta
//
LOCUS       DAZKPO010000117         6080 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000117 DAZKPO010000000
VERSION     DAZKPO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.120"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8NZ_002703"
     CDS             complement(254..541)
                     /locus_tag="ACG8NZ_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0540307.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8NZ_002704"
     CDS             complement(561..785)
                     /locus_tag="ACG8NZ_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0540308.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8NZ_002705"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8NZ_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540309.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8NZ_002706"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8NZ_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0540310.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8NZ_002707"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8NZ_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0540311.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8NZ_002708"
     CDS             3560..3646
                     /locus_tag="ACG8NZ_002708"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0540312.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8NZ_002709"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8NZ_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540313.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG8NZ_002710"
     CDS             complement(4463..4741)
                     /locus_tag="ACG8NZ_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540314.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG8NZ_002711"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8NZ_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0540315.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG8NZ_002712"
     CDS             complement(5597..5977)
                     /locus_tag="ACG8NZ_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0540316.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1610 a         1122 c         1024 g         2324 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaagggctt
//
LOCUS       DAZKPO010000118         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000118 DAZKPO010000000
VERSION     DAZKPO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.122"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8NZ_002713"
     CDS             complement(488..1297)
                     /locus_tag="ACG8NZ_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0540317.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8NZ_002714"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8NZ_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0540318.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8NZ_002715"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8NZ_002715"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0540319.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8NZ_002716"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8NZ_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0540320.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8NZ_002717"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8NZ_002717"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0540321.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8NZ_002718"
     CDS             4179..4913
                     /locus_tag="ACG8NZ_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0540322.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8NZ_002719"
     CDS             4897..5607
                     /locus_tag="ACG8NZ_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0540323.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKPO010000119         5656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000119 DAZKPO010000000
VERSION     DAZKPO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.123"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8NZ_002720"
     CDS             complement(<1..300)
                     /locus_tag="ACG8NZ_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0540324.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8NZ_002721"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8NZ_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8NZ_002722"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8NZ_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540325.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8NZ_002723"
     CDS             1706..1942
                     /locus_tag="ACG8NZ_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540326.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8NZ_002724"
     CDS             1949..3901
                     /locus_tag="ACG8NZ_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0540327.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8NZ_002725"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8NZ_002725"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0540328.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG8NZ_002726"
     CDS             complement(4797..5489)
                     /locus_tag="ACG8NZ_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0540329.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1847 a          981 c          905 g         1923 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaat
//
LOCUS       DAZKPO010000120         5642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000120 DAZKPO010000000
VERSION     DAZKPO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.124"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8NZ_002727"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8NZ_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0540330.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG8NZ_002728"
     CDS             complement(1164..1874)
                     /locus_tag="ACG8NZ_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0540331.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2802)
                     /locus_tag="ACG8NZ_002729"
     CDS             complement(1942..2802)
                     /locus_tag="ACG8NZ_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0540332.1"
                     /translation="MFVAIWGKWSTRRIKMKVLGLGDNVVDKYENLGIMYPGGNSLNF
                     AVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGD
                     RIFLGSNSGGVTREYPIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYD
                     FSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIG
                     YDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVD
                     GSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG8NZ_002730"
     CDS             complement(2768..3736)
                     /locus_tag="ACG8NZ_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0540333.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5642
                     /locus_tag="ACG8NZ_002731"
     CDS             4499..>5642
                     /locus_tag="ACG8NZ_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0540334.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQ"
BASE COUNT         1734 a         1157 c          900 g         1851 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aa
//
LOCUS       DAZKPO010000121         5482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000121 DAZKPO010000000
VERSION     DAZKPO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.125"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..410)
                     /gene="ssrA"
                     /locus_tag="ACG8NZ_002732"
     tmRNA           complement(44..410)
                     /gene="ssrA"
                     /locus_tag="ACG8NZ_002732"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(581..1354)
                     /locus_tag="ACG8NZ_002733"
     CDS             complement(581..1354)
                     /locus_tag="ACG8NZ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0540335.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1498..1848
                     /locus_tag="ACG8NZ_002734"
     CDS             1498..1848
                     /locus_tag="ACG8NZ_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0540336.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1829..2545
                     /locus_tag="ACG8NZ_002735"
     CDS             1829..2545
                     /locus_tag="ACG8NZ_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0540337.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2545..3993
                     /locus_tag="ACG8NZ_002736"
     CDS             2545..3993
                     /locus_tag="ACG8NZ_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0540338.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4063..4572)
                     /locus_tag="ACG8NZ_002737"
     CDS             complement(4063..4572)
                     /locus_tag="ACG8NZ_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540339.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4562..5287)
                     /locus_tag="ACG8NZ_002738"
     CDS             complement(4562..5287)
                     /locus_tag="ACG8NZ_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540340.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1865 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaaataagga tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga
       61 gtcgaacccg cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt
      121 aaggattcac tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct
      181 ctttttcaac ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc
      241 gagcacatgg gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa
      301 gagttgtttt cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct
      361 acgaaacgca attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac
      421 aagggttacg tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata
      481 aaaaagcgaa tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa
      541 gcgattttct taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact
      601 tcaatgcgtg gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt
      661 ttaatccgtc ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa
      721 aatcattcgg accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt
      781 gcagacgttt gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag
      841 gaccattccg ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt
      901 gagataagat cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg
      961 gaacatctga atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc
     1021 agattttctg atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca
     1081 ttttttgaca ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa
     1141 tgaaagaacc attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga
     1201 tttctgcttc tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt
     1261 tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa
     1321 gatcaaagaa agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat
     1381 aagtttatct taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat
     1441 attggttcag tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg
     1501 atttttttca gtattggtat cttattctta ataattggca gtctttttcg gattctgccc
     1561 tcaaaaggaa atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct
     1621 cattggcggc tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg
     1681 gcattgatcg gctattattt aaagacatct gggcatacga attattttct gattgaaatg
     1741 cttctgctag tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa
     1801 tatgacctgg aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc
     1861 tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta
     1921 tgatggaaaa gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac
     1981 actagacctg aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt
     2041 ttcaagtact gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat
     2101 cggcttagag atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat
     2161 tgctcgaatg aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt
     2221 agtttcagat gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg
     2281 agaagcgtat ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt
     2341 gtttacttta gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt
     2401 ctgggattat cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg
     2461 acaaaaaatc gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa
     2521 attcgatgat tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc
     2581 ggaatggtcg cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa
     2641 acgattgaag aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca
     2701 acgcaaacct atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca
     2761 ttgctgctga cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa
     2821 aacgaacgtg tcatttatcc gactaataca gctttactga attttacctt aacagctgat
     2881 caatggaaaa atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta
     2941 ggacaagcac aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat
     3001 ggagcacttg tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca
     3061 ctgaacctgt tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct
     3121 tttgcagcat tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg
     3181 aatggtaatt ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca
     3241 gatgatttca ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa
     3301 gaaaatcgtc gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg
     3361 acaatcaacg gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa
     3421 aacgcgatta aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac
     3481 ttggattatg aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga
     3541 acagaaacat tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat
     3601 acattgacat tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc
     3661 caaatcatgg tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg
     3721 attactttag aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga
     3781 atgtctgaac aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga
     3841 aaaaatacaa aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc
     3901 ttgcataaag gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt
     3961 cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa gcggtataaa agatcatgaa
     4021 tcgatcttct ataccgcttg tttttactat aaaatgattg atttagcccg cgggctctaa
     4081 acgatcaata taaggacttt gagcaatagt agcatcatga gtaaagagta agataccttg
     4141 attttcttca tgagctaaat aagaaagcaa gtggagtagc tcttggatct ccaatacact
     4201 aagttcatca aatgtatctt caataacaat attgtttcgc ttggaaagca aggcatgaag
     4261 taattgcagc tttacttttt caaaagacgt caactcagct gccgcacaat taagcaagga
     4321 aggagagata tcaagttttt cttgataggc caaacgatct tgtttgattt ctttttccgg
     4381 tacatccaac aggacatggt ctttaagatt cagataagga aacatcggcc aatttttttg
     4441 gatgatcgca aaattatcca gttttgcaac aagaacctct tttttcaagt gctccaatat
     4501 tttatgctgt ttttcgagag gacacaaaat aacggtcaag tgttcagcat gaatcaaata
     4561 ttcaggtttc attacgtcca ggcctcctac tttgcaaatg atggataaaa aaagtgaaag
     4621 ggaatagact aagaatgaga cagcatccac tgagcccaat aagagtttgg gcaaatccct
     4681 taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat
     4741 ccatcggaat tgtaagtccg cttaccgaag tcaagatatc acggatatct ggcagttcag
     4801 tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc ttcataaact gactggaagc
     4861 ttacgatcca caataaaacc aagatacaac aaagaaagag aggaaagaac agctgccaga
     4921 aaataaaaag gaataatttg gttttagaaa gaccagtcag gtgccaattg atcaattcct
     4981 gcttgttttg cctgactgaa tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa
     5041 ataaactgaa tatgcctttt atataaaatc ggctagctaa ttctaaagcc ggaagaagct
     5101 ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg gacttgattg gctaaagccg
     5161 attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag cttgaaaata
     5221 aacaatagag tattgtctgc tttttgtaat aaattaatcg agcagtagca tagcggatta
     5281 ttttcatact tttcacctct ataaaaagta tagaaaatga atatgaataa agtatgaaat
     5341 tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg
     5401 aagaagctgc cctattctat taccgtagat gcaagctttt gaacactgtt tattcgaaaa
     5461 gaggagcagc gacaataatt tt
//
LOCUS       DAZKPO010000122         5350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000122 DAZKPO010000000
VERSION     DAZKPO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.126"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1119
                     /locus_tag="ACG8NZ_002739"
     CDS             112..1119
                     /locus_tag="ACG8NZ_002739"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0540341.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1131..2135
                     /gene="neuB"
                     /locus_tag="ACG8NZ_002740"
     CDS             1131..2135
                     /gene="neuB"
                     /locus_tag="ACG8NZ_002740"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0540342.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2132..3280
                     /gene="neuC"
                     /locus_tag="ACG8NZ_002741"
     CDS             2132..3280
                     /gene="neuC"
                     /locus_tag="ACG8NZ_002741"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0540343.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3253..3930
                     /locus_tag="ACG8NZ_002742"
     CDS             3253..3930
                     /locus_tag="ACG8NZ_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0540344.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3881..5062
                     /locus_tag="ACG8NZ_002743"
     CDS             3881..5062
                     /locus_tag="ACG8NZ_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0540345.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5075..>5350
                     /locus_tag="ACG8NZ_002744"
     CDS             5075..>5350
                     /locus_tag="ACG8NZ_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0540346.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEI"
BASE COUNT         1980 a          687 c         1047 g         1636 t
ORIGIN      
        1 atgttgattc gttacgtttt gacatcatta ttgataatta gtttagttat atatttttta
       61 aagattaaga atcgaattat agatgactat aattcatagg aggagatata agtgtttaaa
      121 ggaaaaacaa tattagtaac tggcggaact ggttccattg gaagtgaaat tgttcgtcag
      181 cttttaaaat atgaaccaaa agctatcaga atttatagta gaggtgaaga aaaacaattt
      241 tatatgcaac aagagctctc aaatcacact aatttgagat ttttaattgg tgatatcaga
      301 gactctcaaa ggattgatta tgcatgtcgt gatgtggata ttatttttca tgctgccgca
      361 atgaaacacg ttccagcaag tgaatataat cctatggaag ctgtaaaaac aaatgtgatg
      421 ggaactcaaa atgtaataga tgcagcgatc aaaaataatg taaaacattt tgtaggtatc
      481 agtacagata aagtagtgag tcctactaac accatgggtg cgacaaagtt attgagtgaa
      541 aaattaataa tagctgccaa tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta
      601 aggtttggaa atgtcatggg gtcaagtgga tctgttattc ctctttttag taaacaaata
      661 aaaaacaata aagatattac tttgacagat gttgatatga cacgatttat gatgtcaata
      721 ccggatgctg caagattagt tctgtctgct gctatgaatt caaaatttgg agaaacatat
      781 gtcttaaaaa tgccggcaat caatatttta actttagctc aatcattaat aaaaatatat
      841 aatgatacac atgatgaaaa atatagaggt gaaataaaag ttgtaggtat caggccagga
      901 gaaaaattat atgaagagct aatgactaca gacgaaattg aacgctcata tgaaaatgat
      961 aaaatgtata tcattccacc acactatcat gctactgacg aactgtacaa agattatgaa
     1021 aaaattaaaa tcactgcata ctcttcagat gcaacagaat taatgagtgg acaggaattt
     1081 attagaatcg caaaagagaa taatgtcgtg acttggtaag gagttttatg atgaaatcag
     1141 aagtatatgt aattgcagaa attggtgtaa accataatgg aagtctagat ttagctttag
     1201 aaagtattga taaagctaaa gaatgtggag ctaacgcggt taaatttcaa acatttaaaa
     1261 cgaataagct agtttccaaa aaggctccaa tggcaaaata tcagaaggat aatcttaaag
     1321 aggacagttc tcaatttgaa atgctaaaaa aattagaatt atctcaatct gattttatta
     1381 gaataaaaga ttattgtata caaaataaaa ttgatttttt gtctactcct tttgatgaag
     1441 aaagtgcaga ctttctaaaa caaattggtg tacaaggatt taaaattggc tcaggagatt
     1501 taacaaattt accttttctt agaaaaatag atatgtatgg gttgccagtt ttattatcta
     1561 caggtatggc tgatattcat gaggtcagtg aggctgtaga ttgtttcata gatagtccag
     1621 ttactatttt acattgtact tcaaattatc cggctagttc tgaagatatt aatttattgg
     1681 cgattaacac attaaaagaa atgttcggga tacccgttgg gtactccgat cattcattgg
     1741 gatatgatgt tggtatttgt gctgttgcga tgggtgctaa ggtaattgag aaacatttta
     1801 ccttggataa ggaactacct ggtccagatc ataaagcttc tttagatcct caggaatttg
     1861 cagattttgt aaagcatatt agaaacactg aaatattttt aggtgatggt gttaagcgag
     1921 caatgccttc tgaacttagt acaaaagaag tatcaagaaa aagcctagtg gtcactgaga
     1981 atttaaatgt gggagatatt cttacagaga aatctcttac aataaaacgt cctggaaatg
     2041 gaatagctcc taagttttta tatcaatata ttggcaaaaa agttaaaaga ccaataagtg
     2101 cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa atacttatag taagtggaac
     2161 acgagctgat tatggaatat ataaacctgt tgctgagaat ttactacaag ataattggga
     2221 tgtatcattt gttgtttctg gaatgcattt atcccataaa tatggattta ctaaagaatt
     2281 gatcttagct gacggattta gaattagtgg agaagttcaa agtttgtttt tagagaacaa
     2341 taagggaaac atggctcggt ctgtggcatt agagataaat ggtttcacaa atatatttga
     2401 acaaactagc cctgattttg tattattact aggggataga ggagaaatgt tggcagctgc
     2461 gatagcttgt ttgtatcttg gaatcaaaac agcacatatt cacggaggcg aagtgtctgg
     2521 tactgtagat gaatcaataa gacatgccat aacgaaaata gcatctgttc attttgctgc
     2581 tacggaaaaa agcaaagagc gtttaataaa aatgggggaa gattcttgga gagtttttcc
     2641 cgtcggagct cctagaatag atactatttt aaataataga ttacctagtt ttagagatat
     2701 aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac gttttgcaag tatttcatcc
     2761 agttgtaacc gaactagaaa atatagaaaa acaaatttta gctttgatca attcaatgga
     2821 acaacaatct caaccaatta tttgcatttt acctaatagt gatgctggta gcgaaattat
     2881 cagaaaaatg taccagaaat atttaaaaac ggatattatt tatattacga atttagagcc
     2941 ggaggaatac ctgactatat taaaagagtg caaatttatg atcggaaatt cttcttctgg
     3001 gataattgaa gctgcatcat tcaaaatacc agtaataaat ttaggaacca gacaaaatgg
     3061 acgagaacaa tctaaaaata ttataaatgc aaatctaaat gaaaatgaaa ttgaaactgc
     3121 tttgaataaa atacaagatc ctcagtttat aaaaagcttg aaaaatgtta agaacatcta
     3181 cggtgacggc aacagttcta gaagaataac aaaaacattg aatcaaatat tgaatgatgg
     3241 ggaagatatt aagtggattc aaaaacaaat agcttattaa aatatactag tgatccatac
     3301 attattattg gggctggcgg acacgcaaaa gtcattattg aaaccttaag attagaaaat
     3361 aagttaatat atggattttt tgatgataag caaaataaaa tgcaagatct gaaatatctt
     3421 gggaaaataa acgaattgaa tagctgggcg gatagcaaaa aaaaaatcta taaatttatt
     3481 gtagctattg gtgactgtgt agtaagaaaa aagattataa aaaaattgaa tatgccaagt
     3541 gagcaatatg gaatagctgt tcatccaagt gctatagtgg caaaagaaat ttcaattggt
     3601 tatggaactg ttatttttgc gaatgctgta attaacccag atgctgtaat tggagagcat
     3661 gctataataa acacaggtag cattgttgag catgattgta gaattaataa ctatgttcat
     3721 ttatctccag gtgtatgctt agcgggtgga gtacatgtag gtgtaggaac tcaaatagga
     3781 attggttctc aatgtatcca gatgaagaaa attggatctt ggtgtatgat aggcgcaggg
     3841 agcactattg ttaaggatat tccctcacat tccttagctt atggcaatcc agctaaaatt
     3901 aagaaagaag gaattaattt tgaattctag aattttatta tcaagtcctc atatgggagg
     3961 aacagaagaa aaatatgttg ctaaggcttt taaagataat tggatagcgc cattaggtga
     4021 aaatgtggat aaatttgaag aaagtattac aaaatatact ggttctaagg gcggagcttc
     4081 tgcgttaagt tcaggtacag ctgccataca tcttgcctta agactgctta atgttggaaa
     4141 aaacgatatt gtctttgctc cttcatttac ttttatagct acagtaaatc ctattttata
     4201 tcaaggggct gtaccagtgt tcattgactc agaacctaat acatggaata tgagccctgt
     4261 tgcactagag agcgcattag aagaagctga aagaaacaac caattaccta aggcaatcat
     4321 cgtagtgaat ttatatggtc aaatggctga ttatgaacga attttagcta ttgcaaacca
     4381 atttaatgtt ccagtgatag aagatgcagc ggaatcgtta ggttctactt ataagggaat
     4441 ttcgtcaggc ttatttggga caattgggat ctattcattt aacggtaata agatcatcac
     4501 aacctcaggt ggaggagctc tagtgtctag tgataaaaaa tatgttgagc attcaagatt
     4561 tttagcaaca caagcaagag atcaagcacc gtattatcaa catagcgaaa taggttataa
     4621 ttaccgtcta agcaatattt ctgccggtat cggacgtgga caaatggaag tattgaatga
     4681 tagagtagct aaacgaagag aaattttcaa tcgatataag gaagcttttt ctggaattga
     4741 tagaattgaa atgatgccag aagtggatgg catggtgcca aatcgttggt tgtctacttt
     4801 aacgatcaat gattataatt ctattgatca agtagttaac gtaataaata aactaaatga
     4861 aaaaaacatt gaaacaagag ctttgtggaa accactacat ttgcaaccag tatttcaagg
     4921 ggtaaaaatg tattaccatg ataataatct tagtgtgtca gaaaagttat ttaatagcgg
     4981 cttatgctta ccttctggaa gtaatatgac agatagtgaa caagaatttg ttattgatga
     5041 gtttaaaaaa atattcaagt aggtgtgata ttttatgaac ataggaatta ttggtttagg
     5101 ttccatgggg aaaagaagac ttagactcat tcaagaacac tttaatgata ttttactatg
     5161 tggtgttgat actaatgagg cgagacgaaa ggaagttgaa aaggaatttg gaataccaac
     5221 ttataatagt ctaacagagg caaaaaattt gtttgaaata aattcagttt ttgtttgtac
     5281 atcccctata acccatgaat ttattataaa agaggcttta aatttagatt gtaatatctt
     5341 tacggaaatc
//
LOCUS       DAZKPO010000123         5311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000123 DAZKPO010000000
VERSION     DAZKPO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.127"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8NZ_002745"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8NZ_002745"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0540347.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8NZ_002746"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8NZ_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540348.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8NZ_002747"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8NZ_002747"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0540349.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8NZ_002748"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8NZ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540350.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8NZ_002749"
     tRNA            3917..4000
                     /locus_tag="ACG8NZ_002749"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5311)
                     /locus_tag="ACG8NZ_002750"
                     /pseudo
     CDS             complement(4240..5311)
                     /locus_tag="ACG8NZ_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAZKPO010000124         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000124 DAZKPO010000000
VERSION     DAZKPO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.128"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8NZ_002751"
     CDS             137..748
                     /locus_tag="ACG8NZ_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0540351.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8NZ_002752"
     CDS             821..1750
                     /locus_tag="ACG8NZ_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0540352.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8NZ_002753"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8NZ_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG8NZ_002754"
     CDS             complement(2693..3895)
                     /locus_tag="ACG8NZ_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0540353.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG8NZ_002755"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG8NZ_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG8NZ_002756"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG8NZ_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0540354.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKPO010000125         5176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000125 DAZKPO010000000
VERSION     DAZKPO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.129"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG8NZ_002757"
     CDS             complement(136..1098)
                     /locus_tag="ACG8NZ_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0540355.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG8NZ_002758"
     CDS             complement(1100..2539)
                     /locus_tag="ACG8NZ_002758"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0540356.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG8NZ_002759"
     CDS             2724..4670
                     /locus_tag="ACG8NZ_002759"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0540357.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="ACG8NZ_002760"
     CDS             4934..>5176
                     /locus_tag="ACG8NZ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0540358.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DAZKPO010000126         5137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000126 DAZKPO010000000
VERSION     DAZKPO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.130"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACG8NZ_002761"
     CDS             complement(<1..447)
                     /locus_tag="ACG8NZ_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540359.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="ACG8NZ_002762"
     CDS             complement(754..1851)
                     /locus_tag="ACG8NZ_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0540360.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="ACG8NZ_002763"
     CDS             complement(1959..3986)
                     /locus_tag="ACG8NZ_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0540361.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4859)
                     /locus_tag="ACG8NZ_002764"
     CDS             complement(4131..4859)
                     /locus_tag="ACG8NZ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0540362.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DAZKPO010000127         4939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000127 DAZKPO010000000
VERSION     DAZKPO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.131"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..696)
                     /locus_tag="ACG8NZ_002765"
     CDS             complement(334..696)
                     /locus_tag="ACG8NZ_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540363.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ACG8NZ_002766"
     CDS             complement(770..1150)
                     /locus_tag="ACG8NZ_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0540364.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="ACG8NZ_002767"
     CDS             1232..2407
                     /locus_tag="ACG8NZ_002767"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0540365.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="ACG8NZ_002768"
     CDS             complement(2484..3068)
                     /locus_tag="ACG8NZ_002768"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0540366.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="ACG8NZ_002769"
     CDS             3269..4267
                     /locus_tag="ACG8NZ_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0540367.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="ACG8NZ_002770"
     CDS             4379..4888
                     /locus_tag="ACG8NZ_002770"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0540368.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1569 a          923 c          857 g         1590 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttctttttt
//
LOCUS       DAZKPO010000128         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000128 DAZKPO010000000
VERSION     DAZKPO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.132"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8NZ_002771"
     CDS             85..972
                     /locus_tag="ACG8NZ_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0540369.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8NZ_002772"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8NZ_002772"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0540370.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8NZ_002773"
     CDS             2274..2720
                     /locus_tag="ACG8NZ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0540371.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8NZ_002774"
     CDS             2993..4345
                     /locus_tag="ACG8NZ_002774"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0540372.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8NZ_002775"
     CDS             4516..4803
                     /locus_tag="ACG8NZ_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0540373.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKPO010000129         4757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000129 DAZKPO010000000
VERSION     DAZKPO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.133"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8NZ_002776"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8NZ_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0540374.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8NZ_002777"
     CDS             724..1878
                     /locus_tag="ACG8NZ_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0540375.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8NZ_002778"
     CDS             2093..3061
                     /locus_tag="ACG8NZ_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0540376.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8NZ_002779"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8NZ_002779"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0540377.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8NZ_002780"
     CDS             4091..4699
                     /locus_tag="ACG8NZ_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0540378.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAZKPO010000130         4749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000130 DAZKPO010000000
VERSION     DAZKPO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.134"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..934
                     /locus_tag="ACG8NZ_002781"
     CDS             125..934
                     /locus_tag="ACG8NZ_002781"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0540379.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            974..1471
                     /locus_tag="ACG8NZ_002782"
     CDS             974..1471
                     /locus_tag="ACG8NZ_002782"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0540380.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1856..1929)
                     /locus_tag="ACG8NZ_002783"
     tRNA            complement(1856..1929)
                     /locus_tag="ACG8NZ_002783"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1893..1895),aa:Ile,seq:cat)
     gene            2116..2439
                     /locus_tag="ACG8NZ_002784"
     CDS             2116..2439
                     /locus_tag="ACG8NZ_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540381.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2603..2857
                     /locus_tag="ACG8NZ_002785"
     CDS             2603..2857
                     /locus_tag="ACG8NZ_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540382.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            2927..3172
                     /locus_tag="ACG8NZ_002786"
     CDS             2927..3172
                     /locus_tag="ACG8NZ_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0540383.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            3248..3613
                     /locus_tag="ACG8NZ_002787"
     CDS             3248..3613
                     /locus_tag="ACG8NZ_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0540384.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3674..4237)
                     /locus_tag="ACG8NZ_002788"
     CDS             complement(3674..4237)
                     /locus_tag="ACG8NZ_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540385.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         1507 a          833 c          794 g         1615 t
ORIGIN      
        1 ttcttaagaa tgttttttga aaaaattcat aatgacaaag caagttagtt tcattttttt
       61 aaattctcat ttaatcttaa ggtaacagga aaatagatat gtaaaaagaa agtaggaaga
      121 taaaatgaaa ctagcagcga ttgatttaga cggtaccttg cttgatcgaa atggaaagat
      181 accagaagaa aatatccaag cacttcggaa atttgatgaa aatggcggaa tcgtaaccgt
      241 cgctaccgga aggaacagca tttctgctaa agatattttt tccgaattag gaatcggtgg
      301 ctatttgatt tcttccaatg gttcactgat tgctgaaatg aaggatggaa aaatggcgaa
      361 agttctcaga cgttcaaaaa tagatagcga tacactgaga aaagccttct tctatgctca
      421 agaagcgaaa atatccatca ttgctagccg agaaacccaa gatgatcagc tgacattcga
      481 tgaatcaaat ctagtcaaag atgaccctta ttatgaacat ttcaatttac agaaccattc
      541 ttttgatcaa attttaaata tgttggaaga tccttctctt agctatctta aattagcatt
      601 aacagataag agtgaagaaa aactacagaa gctacaaaga gatctgcaag cagatggtat
      661 ccaatcagta ttctctgatc cgcatttctt agaaatcacg cctacaggta tcactaaagc
      721 tcattcactt gtttttctgg cggattacct tggaatcgaa tcgagtgaca tcatggcttt
      781 tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc gggttgagta ttgcgatggg
      841 gaatgcacag cgacaagtaa aagaaattgc aaaccaagta acactgacga atgaagaagc
      901 tggtgttgct tatattttaa aaaacttcta ttaaaaagaa taaaatgacg atatgaatgt
      961 aggaggaaag aaaatgttag ttattattga tatgcaaaat catattcttg atccaaacag
     1021 cgaattttat attccagagt cagatcaatt ggcagaacgt attgctcaaa gattagctaa
     1081 agcacgccaa aataatgaat atattctctt cacacgggat ataccagttg aactgaagga
     1141 caaagaagaa gaacgagaag atttgcaatt gatccctctt ctttctccac aagaaaatga
     1201 acgagtgatt aaaaagtatt actttacgat tccacctgaa actttagctg aaatccagga
     1261 aactttattt gaaagcaaag aagaacaaaa agagatcgaa gtagtcggga ttgaaacaaa
     1321 cttgtgtgta ctctctaatc tgataggcct gcagagtgct tttcctgaag cagacttttt
     1381 tgttgatcca atgttagtct ccagtagaaa acacggagaa tccgctttag agctgttgaa
     1441 agatttcaac gtctctatcg tcgaatcata aaaatatcta tcttcattta cgatatttaa
     1501 cacacaataa aaaaagcacg cagaactgtt ttagtcctac gtgctttttc tatcaaagta
     1561 gattggcttt tttatgacac agagtgaaac ggcaggctca cgacctcttt cttaaatggt
     1621 gttcaatcag ctgaccctcg gtattagctt aacaaacggc aaaatatgag gaactatttt
     1681 tccgtttgtt aagctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
     1741 gtattcaaaa gcttgcatct gcagtaataa gaacagccaa gtaaaaacat aagaagctgt
     1801 ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttatgtac
     1861 cttgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctaa ccaactgagc
     1921 taaaggtaca tcataaataa aatttatttt tgttactttt caactatagc acgaatgaca
     1981 aactctcaca agtttcaaaa tataagtaaa ataatattgt tattcttatt tcttttactt
     2041 gtgtcataac tattttgtta ctttaatttt tatttgctta ttttagaaaa aatgttattc
     2101 ttttatttat tctcaatgtt aggagtgatt attttgccat ttattgcgat tttattcgat
     2161 ttagttgcgg cagcagctta ttttctccaa ttaaattatc aaacctcagg aattctgttt
     2221 attggaatga tttttcaagg tgtgattacc cttttgcttc ttgtcatgat gctcagctat
     2281 aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg ttaaatatgt ttccattcgc
     2341 tatgggatca tcattctttc ctttttagtg aatgcgatcg tcctcttttt atatgtattg
     2401 aattacttag atatcaatcc attgattttt tctcgataga taagacacat tcagcggaac
     2461 agttcctgct tatcagcaga aaactgttct gctttttcat ttttcattga tctattcttc
     2521 ttttattttc ctagcatttc atttgtgatg tatgcaataa catgttatct tttattatat
     2581 gaatcgttga ggagtccgtt acatgattac gttggtgaga gttttgtttt gggtgcctgc
     2641 ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg aacaaagaac ggttttattt
     2701 agctattttg actcttccgg ctatctactt tatgtggaaa gtttttaatt acaactactt
     2761 tgaacccgat agtgttttta tagaagaatt atcaggactg gttttgtctt tattgatcgt
     2821 cattctttat ttgatacgtt taaataaaaa acattgagta ctattgattg catgtgtagc
     2881 tttctatagt cattataact attctactat aatgaaaggt gattaaatga gtatcgaaga
     2941 agctatttta ctggatactt tacgttgtcg tctgaatttc ttaagaactg gccgatcttt
     3001 atctcaagta gctgtttcta gcgcgttaag tattccttta ttcaaatatc gtaagtacga
     3061 agaaggagaa gaaattccaa gcatcattga attaatctat atcgctgact tttacgatat
     3121 ttccgttgat tacctaattg gtcgctcaga aacagcaagc aaactgaagt gattttttcc
     3181 agccttggtg ctggtttttt catttaacgt ctaaggttaa caatctatat ataaaggaga
     3241 catttctatg cgattatggc atgaaaaatt gatttcaaag cttcctcgtc aacagctttt
     3301 aggacaacat cgggaatgct gtgcattaag aggaaatggc tggggaaaga aacatgctac
     3361 tgtggattat gtttttcact attctcctta tagattattc caatatcatc agcttgtcat
     3421 ggatgaaatg gaaaaacgtg gttacagccc agcaccagag tggaaaaatc ctatgtatcg
     3481 tggcaaatca tgcgaagcat tttttgattt acctactgta gagcgcaacg tgccgattta
     3541 tccggaacac gatgaagttt atctagcaga atgtttagca aatttggagc aaaaaggaat
     3601 ttatcttacc taatataact agaaaagcct tctatctcct cttacttcat taaaaagctt
     3661 taaccatctt agatcaagct aaaatgtcgt aagttttagg gttatcttca tcctcacgtg
     3721 caaaactaaa agcgtactct agttttggtt catcgccaac ttccctagac ataaatgaac
     3781 atctcctgac gtggtttaaa agtaccgcat cttcgacgtc tgtatacgat gcacagcaca
     3841 gattgcttct tttgatcttt cttttgattt ttctgttgct tcttctacac cttcttcgat
     3901 tactacatcg atgcttacaa ttgcttctgc ctaatattcc catgcatcgt cgatttaagc
     3961 ctctgtagtc actgtgatcg tttccacatt ttaactcctc tgtcgtggct tcagttgatt
     4021 ttgtgatcag ttcagttgct aagccagagt cagaacaacc tgccattgct agcgttaata
     4081 ttgatagtaa aaatagtttt tttatcataa ggttcccccc ctgaaaatag actaaaataa
     4141 taacgctttc tttttttgtt ttcagacgaa aattaataat ttctaaatat tctttaaaaa
     4201 agcttataac tattatgcct tttttaatat ataacacagt tatttttaaa aataagaatg
     4261 ctaaatcact ctacaataac ggcgaaagca caaaaaataa aaaaattatt acgttattaa
     4321 aaaaacagat gaaagatcag gttttattag atctcattct ttcatcactt gaagggattt
     4381 aatgaactac tttttcctct ttgtcgtttt tatatattcg tattccttca aacagtagga
     4441 tagcctcttt caatgcttga tatttgaact gtccgttact taaaaattta gatgtgccgc
     4501 cctcttccta gaacatattc ttttttactt tttcattaat tgacagacca atcgaacgaa
     4561 actgtaaatt tcgataggat aatgattggt caaactcatt cattttcaag acatctcgat
     4621 tcatattcaa gataggaatc tttttatcaa catcacttta ttcgtatttt cctctaccat
     4681 aaaagcttca cgctccacaa aattgtttta tacactacta ttcagcccat tcaacgacca
     4741 atgaatttt
//
LOCUS       DAZKPO010000131         4434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000131 DAZKPO010000000
VERSION     DAZKPO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.135"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8NZ_002789"
     CDS             complement(543..2048)
                     /locus_tag="ACG8NZ_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0540386.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8NZ_002790"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8NZ_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0540387.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8NZ_002791"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8NZ_002791"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0540388.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZKPO010000132         4412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000132 DAZKPO010000000
VERSION     DAZKPO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.136"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1132
                     /locus_tag="ACG8NZ_002792"
     CDS             143..1132
                     /locus_tag="ACG8NZ_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0540389.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPRMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAISEEKNYQFIF
                     MTLTTPNVKADKLNEEIDLFNHALKKMFMRKRVKTAIKGYVRKLEITYNKKRDDYNPH
                     FHLILAVPKHYFTVKKYYIKQSEWLDFWRDVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFEMTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYKSGELDHY
                     KKKDENEYFWFLSASWMKKESKYAVDYREMSAEERDYFSGMYVEEKSVEGENE"
     gene            1129..1548
                     /locus_tag="ACG8NZ_002793"
     CDS             1129..1548
                     /locus_tag="ACG8NZ_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540390.1"
                     /translation="MTDSTDKMTIKELADELGITKNTVRNYLVKAGYDLARYKKNGII
                     MLDKELIGVVWSIYRSKGGQLTGNLSVNKAGNDFPLEKENEFLREQLVEKDKQISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1557..1865
                     /locus_tag="ACG8NZ_002794"
     CDS             1557..1865
                     /locus_tag="ACG8NZ_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540391.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHRHLAQTTDEAEKEFWLKLMSHVMQLNQISVINSKEKGM
                     "
     gene            1869..2231
                     /locus_tag="ACG8NZ_002795"
     CDS             1869..2231
                     /locus_tag="ACG8NZ_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540392.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKTVDNEWFADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVNLR"
     gene            2452..3858
                     /locus_tag="ACG8NZ_002796"
     CDS             2452..3858
                     /locus_tag="ACG8NZ_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0540393.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQVG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVQYYKEFQERNPNLAIYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTSVKERESVSDELRYISRQREE
                     NEREKALVASERAEIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            4134..4253
                     /locus_tag="ACG8NZ_002797"
                     /pseudo
     CDS             4134..4253
                     /locus_tag="ACG8NZ_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         1547 a          615 c         1005 g         1245 t
ORIGIN      
        1 aaaaaaaaga ttgcttttcc aatatggttg atttatagtt ttaattgact agaaaaaaac
       61 taaatcagaa tggagagcaa tctatttttt gttatcggct tttcagcctt tttatacaaa
      121 attgtaggtt cagtatagca agatgaaaaa gaaaaagcaa gatttgatta acggaacatt
      181 tcgagaaaaa aagaaaaatt tgaaaaaagt ggtggcattt agtgataatc gaatttctcc
      241 cagaatgcgt gaattgatga caggctgtgg ttcatattta gaatttattg caacgattga
      301 taaggagaaa aagaaattgg tccaagctca cttttgtaaa aatcgtttct gtccgttatg
      361 tgcttggcgt aaagctcgta aggattcgat gatgttgtcg attatgatgc aggcaatcag
      421 tgaggaaaag aattaccagt ttatttttat gacgttgaca acgcctaatg taaaggcgga
      481 caagctaaac gaagaaattg atttatttaa tcatgcgttg aaaaagatgt ttatgcgaaa
      541 acgtgttaag actgcgatta aaggctatgt tcggaaattg gaaattacgt acaacaagaa
      601 acgtgatgac tataacccgc attttcactt gattttagcg gttcctaaac actatttcac
      661 tgtcaaaaaa tattatatca agcagtcgga atggcttgat ttttggcgtg atgtgaccgg
      721 aaagacagga atcaatcctg atggtacaga cgaaattact cagttggata ttcgtaaggt
      781 caaaggattc caacaagaaa aagcggtgca agaagttgcc aagtattctg cgaaagactt
      841 tgaaatgacc gagagccaag aagtctttga cacgttttac catgctatga aagggcgcca
      901 gttaatcacg tttaacggtg tcttcaagga atacaagaaa aaatacaaga gtggcgaact
      961 cgatcactac aagaaaaaag acgagaacga atatttttgg tttttgtctg cttcatggat
     1021 gaaaaaagaa tcaaaatatg cggttgatta tcgtgaaatg agtgctgagg aaagagatta
     1081 tttttcagga atgtatgtcg aggaaaaatc tgttgagggg gaaaatgaat gactgattca
     1141 acggataaaa tgacaataaa ggagcttgct gacgaactag gaataaccaa aaatacagtg
     1201 agaaattatc tagtcaaagc cggatatgat cttgctcggt ataagaaaaa tggaattatc
     1261 atgctggaca aagagttaat tggtgttgtt tggtcaattt atcggtcaaa aggtggtcaa
     1321 ttgaccggta atttatcggt caataaggca ggaaatgatt ttccattaga aaaagagaat
     1381 gagtttttac gtgaacagtt agttgaaaaa gacaaacaaa tttctgaact aacagaaatg
     1441 attcgtgacc aacaaaaatt gacattactt cagcaacaaa aaaacgatca gttgttacta
     1501 gaaaatcaag aattaaaaaa tcggaaatgg tggcaattct ggaagtgaga tgtgacatgg
     1561 ataaaattga tttgagagaa gaaacagtca atagaattga agtgatgtat gaacggttag
     1621 ctgattattt gaatctttcg gatgaagata attatagtga gatgcttgaa cgaatgatgg
     1681 atgttctcaa ctcggttatc aatgctgatc tagcaaaatc ttcagttttg actaatgaat
     1741 ttgttcatag gcatttagcg caaacaactg atgaagctga aaaagaattt tggttgaaat
     1801 taatgagtca tgttatgcag ttaaaccaaa tttccgttat taattccaaa gaaaagggga
     1861 tgtagtttat ggggatgact ttagcagata ttaaagcaca agaagaaaaa ttgaaaaaag
     1921 aacaggcaaa acttcgtcaa cagaaaaaac gattgattga gaaggaaaat gctgaaatgg
     1981 gtaaggcact tcggaagcat tttggatttg aatcttttga tgattttgag cgttggctat
     2041 cgtcaatttc tgacgatcag caacacgaaa aaacagtgga taatgagtgg tttgctgatg
     2101 aaaaatcagc gccaagcgaa cgagattatc ccaatgaatc tgacggctta ctcggagtga
     2161 taccgagtaa aaaaagtcag atgagtgaga gtaaatttac gtcaatgttt aatgacgtga
     2221 atttacgata agcgattatt ttgcgtgaag caaaataaag cgtaaaagga gttgagagcg
     2281 tgacacgctt tcggctcctt ttttcgttgt cacttgtgac agcgaaatcc ctcggagagc
     2341 gtttgggatt gtgacagtca tgtcacaatc aggaaaacgt tactctttga caaagagtaa
     2401 cagaaaggtt tacaatcttt ttattagcga cagccgaagg agggttttaa cttggggttc
     2461 agtatttcgt tcaaaaaggg aacaaagaat acaagcattt ctcataataa tcgtgagtta
     2521 actgatagcc aaaaaatcaa tgactggcat aagcatattg atttcacaaa atcggatgaa
     2581 aatatctatt tggagcaaac aaatattcgt gaaaaatatg aagaattatt tggtgaagcc
     2641 gtagaaaaat ataatgcaaa gcagaaaaga gccgatagaa aaatcgagga ctatttagca
     2701 aaggttcgca aggataaaaa attggaacca cagcgagaat ttatcgtgca agttggtact
     2761 cttgatgatt ttcgtactac tcgtgatgat ggttcttcta ctggcatatc ggaacaacaa
     2821 gcagaacaaa atcgagtgat tgccaataaa attttggttc agtattacaa ggaatttcaa
     2881 gagagaaacc caaatttggc tatttataat gctgttattc ataatgacga aataagtccg
     2941 cacctacact tgaatattgt acctgttgct gaagggtata aacgtggtgt tcagaaacaa
     3001 ccaagcttta ataaagcctt gcaacagcag ggtttacgag cagataaaga gaatggtcga
     3061 gtgatttgga ataattttag gaatcaggaa gttgagtcaa ttgaacgttt gatggcagat
     3121 tttggttggg agcgtgagtt agtcggtact aacaaaatca aagatgttca cgaatataaa
     3181 gaaatcatga gcgagatttc agaattgcgt caaacgtccg taaaagagcg tgagagcgtt
     3241 tctgatgagt tgaggtatat ttctaggcaa agagaagaaa atgagcgaga aaaggctctg
     3301 gtggcttctg agagagcgga gattgaagaa gagaagaaaa atatgctgaa tgtaaaaaaa
     3361 gagatggcgg tcattttcca aaaagatatg gttcctttag aaagtttcaa gaggcttcct
     3421 gatggttcgt atcatttatc tgggaatgat tttgttgatt tgtatcaacg agctacgaga
     3481 gcaacaaatg ccaagagaaa tgctgataac atgagtgagc aatatttaca gatgcttgag
     3541 caaaatacag aattaaatga gtatgtagct gcattggaag aagagcgtca tttgtctggt
     3601 agaaaacaat tagcagattt acaagtggaa aataaaaaac tgaaagaaga aaatcgctct
     3661 ttacgccagc gtttagagcg attaaaaaac gcatttcaaa aatcgatcac tcgtttgtct
     3721 atccgttttg gtcttgcaga aaaggatatg gggctttctg aggaattaga agtattagag
     3781 caggagaaaa ccttatttta tcgtcaaaac gtctcacagc ataaattaga gcgttctgaa
     3841 gatgagatcg agtggtagtt acgatgtgca gtcaacggta ccggaggcgg tttgtgccga
     3901 ggataaggag ttgacggcta aaccactagc cttttaagct gacatttgct aaaaaatggc
     3961 aagtccattt tttggccaac ttagcgttag cgaagtttgg gggcgccctt ttcaaagggt
     4021 gcggttttat tttttggata tttgcatagc taaagagaag tgctatttaa gagttacgtg
     4081 aacttttgaa aattctttcc agaagtctct ttttctttga ggaggaacaa aatttgagta
     4141 aacgtggcac gatcataaat gagtgggaaa aacttaaccg catttctaac aggcggttaa
     4201 agtatcaaag tttgaccctt cccttaaaaa tgcagtggtc aatcgatcga attttatgtc
     4261 tttgggtttt ggggttttaa aacgaaaaaa agggggcgat tttcgccccc ttttttgatg
     4321 ttttttctta tcttgatact ataggcaact atttctgtga agaaataggg gggtatagta
     4381 tgtctttgac cttggtgcat aagggattgc ac
//
LOCUS       DAZKPO010000133         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000133 DAZKPO010000000
VERSION     DAZKPO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.137"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8NZ_002798"
     CDS             73..234
                     /locus_tag="ACG8NZ_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0540394.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8NZ_002799"
     CDS             253..1530
                     /locus_tag="ACG8NZ_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0540395.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG8NZ_002800"
     CDS             1540..2196
                     /locus_tag="ACG8NZ_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0540396.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG8NZ_002801"
     CDS             2196..3572
                     /locus_tag="ACG8NZ_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0540397.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG8NZ_002802"
     CDS             3702..4115
                     /locus_tag="ACG8NZ_002802"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0540398.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG8NZ_002803"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG8NZ_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKPO010000134         4332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000134 DAZKPO010000000
VERSION     DAZKPO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.138"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            453..599
                     /locus_tag="ACG8NZ_002804"
                     /pseudo
     CDS             453..599
                     /locus_tag="ACG8NZ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(687..980)
                     /locus_tag="ACG8NZ_002805"
     CDS             complement(687..980)
                     /locus_tag="ACG8NZ_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0540399.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1017..1202
                     /locus_tag="ACG8NZ_002806"
     CDS             1017..1202
                     /locus_tag="ACG8NZ_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540400.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1383..2561)
                     /locus_tag="ACG8NZ_002807"
     CDS             complement(1383..2561)
                     /locus_tag="ACG8NZ_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0540401.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2665..2979)
                     /locus_tag="ACG8NZ_002808"
     CDS             complement(2665..2979)
                     /locus_tag="ACG8NZ_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540402.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3086..3301
                     /locus_tag="ACG8NZ_002809"
     CDS             3086..3301
                     /locus_tag="ACG8NZ_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540403.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3601..3843)
                     /locus_tag="ACG8NZ_002810"
     CDS             complement(3601..3843)
                     /locus_tag="ACG8NZ_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540404.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3830..4258)
                     /locus_tag="ACG8NZ_002811"
     CDS             complement(3830..4258)
                     /locus_tag="ACG8NZ_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0540405.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1375 a          827 c          649 g         1481 t
ORIGIN      
        1 taaataaaaa atactctttt tttacgatta ttgaaaaaaa aagataaaac ttgattaatt
       61 gaagtattgc ttaaatttgg aagctatgag ggaagaataa aagagttagg acgggattat
      121 ctactatttt tctttgtttt aaatatttgt tatctttgaa cataaaagag aaatgtgtcg
      181 actgcagtgt tgtgtccctc atcacgaata aacggtgttc aactagctga tcttgtattc
      241 acttaaaaaa tggtaaagta cgagttgcta ttccccattt tcaattttct aacgctcagg
      301 acaaaatact gttttcacaa cctcattgga ataaaagcat atatattgtt tcaagaacga
      361 aatcttgtta tctttttaaa gggcatctgt ccaaaatcta attttaggag gtacgttttg
      421 gtatttattc gtacaaataa aagagaatta tctttattca tgtaacgcat ccaattgatt
      481 ttttcatgta cgctcctgga acgattttac tgacgattgc ttatttaact gcgaatcgct
      541 cattagcttt tatcatagct atccatattt tgaataacgt tcttggcttt gtattataga
      601 tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat acttgaactt
      661 ttttcaatga gtataaatta aaacttctaa ttttcaattc ccatcatctg atatgcccga
      721 ccgtctgaac ttcttttgaa aaagccaaat gtgattagat aacgtctttg ttcgacataa
      781 tcgccactag aaaatacgtt cagcagtatc gaattaacct ctgtttctgt ataagtcact
      841 ccatggtcaa agcgtttcaa gacttctttc aataggatgt atttcttttt tgttgaactt
      901 ggtacttgtt ttacttttcc atcattcatg aatgtgtcta aaactttcat cttttcttgc
      961 tccgtataag tataaaccat cttctttctc cttttgattc taagaatact ccatttatgt
     1021 tagaatattc tggatttttt attaaagcgg ttactcttaa gaaagatatt atcatagtgc
     1081 tctttttttt actactactt ataggtagat ttattctaat ccattattct cccctaccaa
     1141 taattaaaga aaaaactgga tcagggtgtt caaattacat gctaagaaaa aagaaccctt
     1201 gaataccaag ggttctttct cagtctgata acaatgatta acgacggatt tctttgatac
     1261 ttgtgaaaag tcgttaaagt attgatataa aaggattttt attacctctg actacatatt
     1321 gactaatttg ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct ttcaccgttt
     1381 ttttaactct ctaaagaact aaaagcaatc agaggtttta ccttttcatt tgacgttaag
     1441 tggcgataaa ttttcgaagt tgtttggtca gtatggccta agcgatcttt caagacttcc
     1501 cagctacagt gttccgctac taagcttgcc attgtatgac gagctaaatg agtatgaaat
     1561 ggcttattaa ttcctgagcg attcacgcac cgtttgacgt gatcatttac atactctcga
     1621 cgataaggaa caccacgttt actagaatca agatacacaa acaaatatgt ttcttctata
     1681 ataaacctgt aatgttgcct catctcaccc aaagccttaa tcaactgttg tagcttttcc
     1741 ataactagag gagttactag aactcgtcta gcagaactaa ctgtcttagt agtttcaatt
     1801 cgaaaatcct tactcttacc tttaactgcc gtttgcttat agaacagaat ttcatttctt
     1861 ttgaaatcta tatctccagg ctgtagagca agcgcctcgc tgactcggca gccagtgtaa
     1921 aatagaacat ggaaaagtac agacatcggt aattcctcat gaacaatgcc ataattcaaa
     1981 aaagttctca attcatcgac agtaagaaac ttgtcctcaa tattatttat ggcttctttt
     2041 aaatcagtta ccgttaactg atattttggc attctcacag acttcattgg attttcactt
     2101 atcaaattct ttctttgaca atatttaaaa atcatattta gaacttgaat aagtgattgt
     2161 aaactatttt tggcatagcc aacatttgtt ccattaacgc cacattcctt taaggtaaaa
     2221 agcaattctt gaaggcactc tggtgtcaaa tcagacaact ttttatcacc aatccatcga
     2281 acaataattt ttaattgttc ctctcgctta tcataggtta tttccttttg ttcatttaac
     2341 gaacggtaat attggaacca ttgctcacct acaacactaa acttgtcatt gcttgcaaca
     2401 atttttttct taagcctttg ttgtttctcc atttccagat aagcgagttg cgcctcacgt
     2461 tttgaattaa agccacgtct tgttgttcga tatgcttgaa agtaccatgt gatcctacca
     2521 gtagaactga tttgcttctt gaatcgatct gttttttcca tttattgatt cctcctattt
     2581 tgaaggaacc caacaagcag aaaccctttg gaaatctttc tatctatgtc atcattgtac
     2641 cgcatcttag aaagaaaatg taacttataa agatcgaagt agtcgcaatt tttctcgata
     2701 gtcattcgaa tcactctttc tcatccattc taaaacatca tcacgaaaat agtgtcctcg
     2761 aatcccgtac caacagggag gaaactcacc aagttttact aaacggttca gtgtactatc
     2821 acttatctga aatagtcgct ttaaatcagt atttgttaat accagaggta aatgactgac
     2881 tgcaagcaat ttttccgtgt aatcttccag ttttttttgt aaaattgaat agaactctat
     2941 ttccagagaa ttaacatttg ttttaacttc caactccatc acccttctct ctcaaaatca
     3001 aaacgaccac cacggtaccc cttaaaattg taaaaaaaca ctccagtact ccatcaaaac
     3061 ggactagaaa agaggaattt ttaatatgaa acaaggaatt gcagacataa aaataattaa
     3121 agaaattctt gaaaaatcta cggctaatgc aatagcattc ggaacaggaa tcaacttgag
     3181 tacagtaaaa aaacttaaaa gtggagagag agctgaagaa aaattaaatc ttgccgatgc
     3241 tattaaaatc acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa tatggaaata
     3301 gtagtttttc tgatctatca gctgttttgc caaagcacat aggaatctca cctcccccca
     3361 gtaatggcgg gccgatttgg ttacggaagt atcattatcg tagtcgcgat tctcatatta
     3421 aaatttgtcg cttaaaagct aaccttgtca tggcgtaact agagaatcaa aatctacatg
     3481 tactgataga tttgctattc atttgtcaaa gagcgctagg ctattttcag cctatggaaa
     3541 tcctttattt tgacccgata aaaaacttcc atagaatgaa aaggaaaggg aaaaaatact
     3601 ttaacgagtt gtatcaaatt caagtatctt ctcaatcaat tttgtttcca gttgtctacg
     3661 catatattca tctacataca taaccattcc tttttcttct tcataaactg gtttcatggc
     3721 taaccgtgaa atataatttt gatattttga cagaacgatg gacatggcaa ttgaatcacc
     3781 agaagctgcg gctttgattg tcgttagtgg tatgaatggt atatatcgtt tattttgctt
     3841 catatttgac acccaaatac tttcctagct cttccaaagc catttgtcgt tggtaccaaa
     3901 cactgccgag acttttccca ataatatcgc caatttcacg atcattaaga ccgatgaaga
     3961 aggacataaa aatgatttct tgtctttgac taggtaatgc tgcaatcgca tttgccaact
     4021 cctcattgga aataaagatt ttcataccaa ggacaataaa agtaccacta ctattcaaat
     4081 ttggttccaa ataatagaac tctgtaccta accctttttg ttccaatatt tccattgata
     4141 tttgccgttg gcgaaatcta gcgtacttct tttgaatatt taacgcctca ttgcagacta
     4201 ctttcttaca aaaactgtca aacgtatgtt gaatgtgggt ttcctctgag tcgcccataa
     4261 tattctcccc ctcaaaaaat tcattcaact cgttttactt ctatcccttt ccattattac
     4321 tacaatctag ga
//
LOCUS       DAZKPO010000135         4295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000135 DAZKPO010000000
VERSION     DAZKPO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.139"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8NZ_002812"
     CDS             <1..792
                     /locus_tag="ACG8NZ_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0540406.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG8NZ_002813"
     CDS             889..3138
                     /locus_tag="ACG8NZ_002813"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0540407.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="ACG8NZ_002814"
     CDS             3141..>4295
                     /locus_tag="ACG8NZ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0540408.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DAZKPO010000136         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000136 DAZKPO010000000
VERSION     DAZKPO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.140"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8NZ_002815"
     CDS             complement(37..1257)
                     /locus_tag="ACG8NZ_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0540409.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8NZ_002816"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8NZ_002816"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0540410.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8NZ_002817"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8NZ_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0540411.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8NZ_002818"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8NZ_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0540412.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKPO010000137         4011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000137 DAZKPO010000000
VERSION     DAZKPO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.141"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..774
                     /locus_tag="ACG8NZ_002819"
     CDS             178..774
                     /locus_tag="ACG8NZ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0540413.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACG8NZ_002820"
     CDS             898..2697
                     /locus_tag="ACG8NZ_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0540414.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACG8NZ_002821"
     CDS             complement(2975..3187)
                     /locus_tag="ACG8NZ_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540415.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="ACG8NZ_002822"
     CDS             3649..3798
                     /locus_tag="ACG8NZ_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540416.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1179 a          686 c          709 g         1437 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat t
//
LOCUS       DAZKPO010000138         3923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000138 DAZKPO010000000
VERSION     DAZKPO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.142"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8NZ_002823"
     CDS             complement(573..1130)
                     /locus_tag="ACG8NZ_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540417.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8NZ_002824"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8NZ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540418.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8NZ_002825"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8NZ_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540419.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG8NZ_002826"
     CDS             complement(2887..3165)
                     /locus_tag="ACG8NZ_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540420.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG8NZ_002827"
     CDS             complement(3199..3450)
                     /locus_tag="ACG8NZ_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540421.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DAZKPO010000139         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000139 DAZKPO010000000
VERSION     DAZKPO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.143"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG8NZ_002828"
     CDS             complement(245..466)
                     /locus_tag="ACG8NZ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540422.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG8NZ_002829"
     CDS             complement(564..809)
                     /locus_tag="ACG8NZ_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540423.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG8NZ_002830"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG8NZ_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG8NZ_002831"
     CDS             complement(2033..3034)
                     /locus_tag="ACG8NZ_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0540424.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG8NZ_002832"
     CDS             complement(3193..3459)
                     /locus_tag="ACG8NZ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0540425.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG8NZ_002833"
     CDS             complement(3449..3805)
                     /locus_tag="ACG8NZ_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0540426.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKPO010000140         3765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000140 DAZKPO010000000
VERSION     DAZKPO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.144"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..884)
                     /locus_tag="ACG8NZ_002834"
     CDS             complement(459..884)
                     /locus_tag="ACG8NZ_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540427.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1258..2229
                     /locus_tag="ACG8NZ_002835"
     CDS             1258..2229
                     /locus_tag="ACG8NZ_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0540428.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2261..3658
                     /locus_tag="ACG8NZ_002836"
     CDS             2261..3658
                     /locus_tag="ACG8NZ_002836"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0540429.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1291 a          608 c          772 g         1094 t
ORIGIN      
        1 aattactacg tgatgtagaa taagcctttc tatcgtttgg ttattttata ttcaaatatt
       61 tgaaataaat aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta
      121 ctgatgaagc ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt
      181 gagttttgct tggatttgtt tatcttcttc tactttttac ataattttga acaggataag
      241 attcaaatgt tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc
      301 ttccttggtc attaaattct gtatggatat ttttgttgtt attacatgta caataatcag
      361 gactatcgat catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa
      421 aagaagggga gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca
      481 tcctcatcct tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta
      541 ttttcttttc ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc
      601 actgtttttt cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc
      661 cattccattt ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt
      721 acttttagcg tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa
      781 tcggttgttt cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc
      841 aaaagtcccg cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt
      901 gataggataa agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa
      961 cgcacaaaac acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat
     1021 ccgccacttt cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt
     1081 aaaaatgaac cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct
     1141 actgaagact ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc
     1201 atcataatcg aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg
     1261 gcatcgatcc gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac
     1321 ttgaatggtc agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg
     1381 ttggactata aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt
     1441 ggacttttga taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa
     1501 agagtagctg aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa
     1561 aaaattgaac aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc
     1621 ttatccgaag aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc
     1681 gcattgaatt gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa
     1741 agtgtagcaa aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt
     1801 gctgccactc aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc
     1861 caaaatgatc catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc
     1921 tattacgggg caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac
     1981 aatatgtcga ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat
     2041 attgcgttca gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc
     2101 acgatcacgc caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga
     2161 atcaaaaaag aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga
     2221 atcaactaaa acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc
     2281 agcaaatttt tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg
     2341 cgatggaaaa ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt
     2401 tgatcaagta ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca
     2461 gttgatgaaa cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat
     2521 acctgatgaa tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga
     2581 tgagttgatt gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat
     2641 gtatctgcaa gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata
     2701 tgccaaaagt tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga
     2761 accaatcgtt ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga
     2821 tatgaaaaaa gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa
     2881 atcttaccat gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag
     2941 ctatccaaga gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt
     3001 cttcaatcgt tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga
     3061 gatagtaaaa gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa
     3121 agaaaataca gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa
     3181 agaaacacca atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga
     3241 aatgcctggt cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta
     3301 tgatattttg atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa
     3361 cggtatgggt gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga
     3421 ttatcggatc gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg
     3481 tgccaattgt ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc
     3541 atataagaat cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat
     3601 caaaaaatcg ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga
     3661 aagaatagtt gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag
     3721 ttcaattatt tacgatcttt ttcaactttt ttgtctttct atttc
//
LOCUS       DAZKPO010000141         3664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000141 DAZKPO010000000
VERSION     DAZKPO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.146"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG8NZ_002837"
     CDS             <1..277
                     /locus_tag="ACG8NZ_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0540430.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACG8NZ_002838"
     CDS             420..641
                     /locus_tag="ACG8NZ_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540431.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACG8NZ_002839"
     CDS             657..1499
                     /locus_tag="ACG8NZ_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540432.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACG8NZ_002840"
     CDS             1562..3364
                     /locus_tag="ACG8NZ_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0540433.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACG8NZ_002841"
     CDS             complement(3423..>3664)
                     /locus_tag="ACG8NZ_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0540434.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAZKPO010000142         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000142 DAZKPO010000000
VERSION     DAZKPO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.147"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8NZ_002842"
     CDS             <1..186
                     /locus_tag="ACG8NZ_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540435.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8NZ_002843"
     CDS             329..553
                     /locus_tag="ACG8NZ_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540436.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG8NZ_002844"
     CDS             568..1437
                     /locus_tag="ACG8NZ_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0540437.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG8NZ_002845"
     CDS             1418..2152
                     /locus_tag="ACG8NZ_002845"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0540438.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG8NZ_002846"
     CDS             2185..3093
                     /locus_tag="ACG8NZ_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0540439.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG8NZ_002847"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG8NZ_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKPO010000143         3473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000143 DAZKPO010000000
VERSION     DAZKPO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.148"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8NZ_002848"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8NZ_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0540440.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8NZ_002849"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8NZ_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          986 a          763 c          541 g         1183 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataacgtg ttttattttt taa
//
LOCUS       DAZKPO010000144         3276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000144 DAZKPO010000000
VERSION     DAZKPO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.150"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8NZ_002850"
     CDS             complement(451..1761)
                     /locus_tag="ACG8NZ_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0540441.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACG8NZ_002851"
     CDS             complement(1773..3131)
                     /locus_tag="ACG8NZ_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0540442.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
BASE COUNT         1027 a          664 c          506 g         1079 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaa
//
LOCUS       DAZKPO010000145         3240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000145 DAZKPO010000000
VERSION     DAZKPO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.151"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG8NZ_002852"
     CDS             complement(554..2167)
                     /locus_tag="ACG8NZ_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0540443.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACG8NZ_002853"
     CDS             complement(2308..3216)
                     /locus_tag="ACG8NZ_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0540444.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1044 a          556 c          572 g         1068 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
//
LOCUS       DAZKPO010000146         3224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000146 DAZKPO010000000
VERSION     DAZKPO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.152"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..284)
                     /locus_tag="ACG8NZ_002854"
                     /pseudo
     CDS             complement(111..284)
                     /locus_tag="ACG8NZ_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8NZ_002855"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8NZ_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACG8NZ_002856"
     CDS             2090..3130
                     /locus_tag="ACG8NZ_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0540445.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1199 a          448 c          462 g         1115 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataa
//
LOCUS       DAZKPO010000147         3201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000147 DAZKPO010000000
VERSION     DAZKPO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.153"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8NZ_002857"
     CDS             301..585
                     /locus_tag="ACG8NZ_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540446.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8NZ_002858"
     CDS             588..1004
                     /locus_tag="ACG8NZ_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540447.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8NZ_002859"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8NZ_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0540448.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2523..2609
                     /locus_tag="ACG8NZ_002860"
     CDS             2523..2609
                     /locus_tag="ACG8NZ_002860"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0540449.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..>3201)
                     /locus_tag="ACG8NZ_002861"
     CDS             complement(2912..>3201)
                     /locus_tag="ACG8NZ_002861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540450.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DAZKPO010000148         3191 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000148 DAZKPO010000000
VERSION     DAZKPO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.154"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8NZ_002862"
     CDS             <1..1412
                     /locus_tag="ACG8NZ_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0540451.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACG8NZ_002863"
     CDS             1546..2889
                     /locus_tag="ACG8NZ_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0540452.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..>3191
                     /locus_tag="ACG8NZ_002864"
     CDS             3001..>3191
                     /locus_tag="ACG8NZ_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0540453.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSS"
BASE COUNT         1265 a          442 c          609 g          875 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg a
//
LOCUS       DAZKPO010000149         3126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000149 DAZKPO010000000
VERSION     DAZKPO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.155"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1040
                     /locus_tag="ACG8NZ_002865"
     CDS             72..1040
                     /locus_tag="ACG8NZ_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0540454.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1050..1538
                     /locus_tag="ACG8NZ_002866"
     CDS             1050..1538
                     /locus_tag="ACG8NZ_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0540455.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            1834..2115
                     /locus_tag="ACG8NZ_002867"
     CDS             1834..2115
                     /locus_tag="ACG8NZ_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540456.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            2117..2533
                     /locus_tag="ACG8NZ_002868"
     CDS             2117..2533
                     /locus_tag="ACG8NZ_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540457.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1251 a          543 c          475 g          857 t
ORIGIN      
        1 attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag ttaatcttga
       61 aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat acgattcctt
      121 tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct agaatgcgtg
      181 atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta agcaattata
      241 aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac aaattaatgg
      301 atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt gttttattta
      361 atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa gtccatgaga
      421 aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc caacaattca
      481 atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa tacagaacca
      541 aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc cctaaaagtt
      601 atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa
      661 tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag cctgtcattg
      721 gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac aaacatgctc
      781 caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt caatctgaaa
      841 aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta gaacttttac
      901 aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt aacaaacaaa
      961 tagcagataa caattccgat attaccccag aatttaaaga aaacttacta gataatctcc
     1021 aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa gaattagcag
     1081 atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta cctagtaatt
     1141 ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca acaattacaa
     1201 aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat tcattaccta
     1261 ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa aaagataggc
     1321 aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac cagcaacaac
     1381 aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc cagctaacct
     1441 atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact caaaaaggac
     1501 aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt tattcataaa
     1561 aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat ctcacgtcgc
     1621 tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt gctaggtagg
     1681 ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca ttgtggatag
     1741 cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt atgatataca
     1801 taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg gaatattgat
     1861 ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa aactggcaaa
     1921 aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga ccaggcactt
     1981 aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga tatacaagca
     2041 aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat tgaaacactt
     2101 atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag
     2161 tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata actactcgga
     2221 agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa ctaccagcaa
     2281 ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa tcattgcaga
     2341 atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata
     2401 tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa atcaacccaa
     2461 aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc caacaaactt
     2521 tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct tttttagggg
     2581 gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag aaaaatccgc
     2641 ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa tatcggttat
     2701 ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa gactgctcta
     2761 acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc gcttatgaag
     2821 cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa ggaatcggtt
     2881 aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg aacgtacctt
     2941 ttagctagac caatgaaaaa tcacaaagtg agcccctgta tcggttgata caagggctac
     3001 ttggcatact cgacattatg tctactagct cgataaataa cgattatgtt cccaaaacaa
     3061 tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa ttataggtgt
     3121 atatca
//
LOCUS       DAZKPO010000150         3068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000150 DAZKPO010000000
VERSION     DAZKPO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.156"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..1099
                     /locus_tag="ACG8NZ_002869"
     CDS             284..1099
                     /locus_tag="ACG8NZ_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540458.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1225..1482
                     /locus_tag="ACG8NZ_002870"
     CDS             1225..1482
                     /locus_tag="ACG8NZ_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540459.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1734..2306)
                     /locus_tag="ACG8NZ_002871"
     CDS             complement(1734..2306)
                     /locus_tag="ACG8NZ_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540460.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2322..2927)
                     /gene="fic"
                     /locus_tag="ACG8NZ_002872"
     CDS             complement(2322..2927)
                     /gene="fic"
                     /locus_tag="ACG8NZ_002872"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0540461.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1015 a          539 c          473 g         1041 t
ORIGIN      
        1 taagaaattc aaaaaagaaa aaactttcct taaaaacaaa aaataggagt attcagtttc
       61 ttaatagttt tgtaatagtt gtatgtagaa gttttcttaa tagtttttgt aataatttat
      121 taatcgtagt ttatataact tatttgtaag tgactagaga attaaataaa aaataattac
      181 tatctagtac gaaattaatt acataggata atatattaag gtgctggact acaagtaaaa
      241 aatactgtat gatcatgaca gattcaagaa aggagcaata agattggtta agatagcagg
      301 actttctaaa ggtggaaaaa ccgtttcaca actttataaa caaaactaca gagactggag
      361 acagttcaga aaaaacatta aggattctta ctttattcta ccaacagaac ttgaaaaata
      421 tttaccgaat aacaaagcta ttaatttata tctgtattac tgttttaagg caaagaatga
      481 aggcggggat tcctggcatt cggtcaatac catagcagag tctctaggag tgacaactaa
      541 aactataaac acctggaata aaaccttagt tgatttaggt atgattgcac gtatagacga
      601 taaccatctc agtaaatcga caattttact tcctctctct agttactata aattagccct
      661 tgatagttct ttaaaggatg ttaccgagag cactgtccat gaaattgatg gcaacttggt
      721 tgcagtttat catttgtttg aatggagaaa aaatgaggct gatgaaaatt ataacgttcc
      781 ttataacaca ctctgttgtg tttttaaaag aaaaacacaa aaagatacta tctataaatt
      841 tatcttggtc caagatgaga aaataagtaa tttcgaatta agcactcctt ctagtaagat
      901 ttatgacgat gcatatagat ttgaatgtca agatctagaa gccctccttt ccgcaaataa
      961 tctaaacaat atagaacatg aaaactttgt tgtaaacact agatttaatt tagtttctga
     1021 aaaggactca gatttgttag atttgctgat agagcttact aaaaatctag ataagaaaga
     1081 aaacattact gtactataac tagcatcggt ccatcattaa gtttggacta ttagaacact
     1141 tgctattaca gaaaaaggta tgctagccac gcttactatt tattggtatc aaacaagtat
     1201 cttaatttga aaggaagatg atttatggct aatttatata tagagaacaa aggagacttg
     1261 aatgccatta ttactttaat cgtagttatg gtaaaatcaa taggtaatat cacaatagga
     1321 atgctgttta ttatattagc agcagtgctg atttttatga tagttatttg gttcttaaat
     1381 gaaagatcta aagacaatca acaccgtagg gatatggaga taaaatcaat agagtcaata
     1441 gattatttat cgataataac actaaaaaag ccacgttcct aaacgtgact tacgctgaac
     1501 gatatgtgtt gtttggtaga cgaacaaatc gttcattata aatatatgat ggtatcctaa
     1561 tatagtaagc gtggctagca tacctttaat tactttacct tttacagtta ccatacacat
     1621 tttttgagca gaaatcaagt aaataaaaaa atattatatt tcacaacatg ctaatagact
     1681 catatctaga acgcattaag tatttacctg tctggttcgc tgagacttct tttttaactt
     1741 tcttcaattt tcttctttat tctaaaaagc gtactgcgac tgaaaccggt tatagcttca
     1801 acttccttat acgatttacc tgaagtaagc agttcatatg cgtgcctctt cttcggtgtg
     1861 attgttgctt tcggacgtcc ttctctgaaa ttaggattat tctttcttgc aaacattttt
     1921 ccttcttggg tacgttccac aatcatatca cgttccattt ctgctacact caacaacgtc
     1981 cgtgtcacca ttcgccccat ggacgtattt tcaatcgttc ctagattcaa aaccttgatc
     2041 gttacgttat catcgagtaa aggttcaatg atctctaatg cttctctcgt gttacgagca
     2101 aatcgatcaa gcttcgtaac aactatttca tctccaggct tcagaccagc caacagctga
     2161 ttgaattttg gccgatcccg ttttgtaccc gtaaattttt ctgaaaaaat attcttcttc
     2221 tctacgccgt tctcaagcaa ctgttgaatt tgttcttttg tactttgaga agcggttgag
     2281 acacgtgcat atccatattt cgtcatttgg tccacctact tttacaaatc atctatcgta
     2341 tactcagaat acccttcata gaaatagctg acatcgattc ctttcatata taactcgcga
     2401 tcgtcaattt tatcagtcag agcattggaa attagatagc gtatttccaa atcgtttatg
     2461 ggacttcgct ccattgcaga aagatagtct gctttatcga ttaagttcca atctacaact
     2521 ttttgcaatt cttcctttaa aatgagatcc agccaaattc tagtacttcg gccatttcct
     2581 tctcgaaatg ggtgagcgat attcatctcc acatatttct tgataatctc ttcaaaagaa
     2641 gtttgtggca tctgatcaat atgctgtaac gaatgttcta aatacataac tggtgcaaat
     2701 cgaaaatttc cttttgcaat attgaccgat cggatttttc cggcaaaatc gtaaatatcc
     2761 gaaaatagaa attcatgaat ttcagataaa cctttaaatg taccaacttc gatttcattt
     2821 atttttccac aatcgtacaa ttctttcgct ttttgtttac tgagtttttc ttcaacctta
     2881 gccaactcaa cttgattggt taatcccaat ttattctcta aaatcatgcg aaatctcctt
     2941 aactttatga cataagttta tgaaacactc tcaaccccat tatatcgcga ttttgtttat
     3001 gtgtcaaaaa ctattaagtt tctgaccccc gatagatttt tcaagtgtct ctattttgca
     3061 cagtctag
//
LOCUS       DAZKPO010000151         3038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000151 DAZKPO010000000
VERSION     DAZKPO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.157"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8NZ_002873"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8NZ_002873"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0540462.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG8NZ_002874"
     CDS             complement(1034..1954)
                     /locus_tag="ACG8NZ_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0540463.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG8NZ_002875"
     CDS             2067..2819
                     /locus_tag="ACG8NZ_002875"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0540464.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAZKPO010000152         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000152 DAZKPO010000000
VERSION     DAZKPO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.158"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8NZ_002876"
     CDS             85..981
                     /locus_tag="ACG8NZ_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0540465.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8NZ_002877"
     CDS             1073..1288
                     /locus_tag="ACG8NZ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0540466.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8NZ_002878"
     CDS             1306..1578
                     /locus_tag="ACG8NZ_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0540467.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8NZ_002879"
     CDS             1580..2443
                     /locus_tag="ACG8NZ_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0540468.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKPO010000153         2875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000153 DAZKPO010000000
VERSION     DAZKPO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.160"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..531)
                     /locus_tag="ACG8NZ_002880"
     CDS             complement(154..531)
                     /locus_tag="ACG8NZ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540469.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(831..1712)
                     /gene="galU"
                     /locus_tag="ACG8NZ_002881"
     CDS             complement(831..1712)
                     /gene="galU"
                     /locus_tag="ACG8NZ_002881"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0540470.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1755..2774)
                     /locus_tag="ACG8NZ_002882"
     CDS             complement(1755..2774)
                     /locus_tag="ACG8NZ_002882"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0540471.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          793 a          643 c          436 g         1003 t
ORIGIN      
        1 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
       61 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
      121 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
      181 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
      241 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
      301 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
      361 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
      421 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
      481 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
      541 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
      601 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
      661 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
      721 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
      781 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
      841 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
      901 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
      961 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
     1021 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
     1081 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
     1141 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
     1201 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
     1261 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
     1321 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
     1381 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
     1441 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
     1501 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
     1561 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
     1621 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
     1681 gcggcaggaa tgactgcttt tttgaccttc acttgctgtt cctcctaaac gacgattaac
     1741 tattgatcat ctatttaacg aagagaaaat tcattctcca tttttccgtc acgtaacatg
     1801 atttcttttg ctgctttttt gatatcttct ccattataca aaacattata gattgtttga
     1861 gtaattggca tctcaacacc tagctgttga gctagctcta ctgctgcttt tgtcgttgcc
     1921 acaccctcaa cgaccattcc catgttttct agaacttctt ccagcttatg tccctgtcct
     1981 aacaagttac cggcacgcca attgcgagaa tgaacacttg tacaagtaac gatcaagtcc
     2041 cccacaccac tcaaaccgat aaatgtcaga ggattggcac ccatcgcgac accaagacgg
     2101 ctgatttctg ccaatcctcg agtcatgatt gctgcttttg catcatctcc gaatcctaat
     2161 ccatggatag cgccggcacc aattgcaatg atatttttca gtgctgcacc agtttctacg
     2221 ccaatcacat cattatttgt ataaatcctg aaataatcat tcatgaacag tttttgtaca
     2281 taggctgctg ctttttgatc aatacttgca gctgtaattg ttgtgatatc atgtactgcc
     2341 acttcctcag catggctagg accagataat acaacaattg cctgtctttt ttcttctgga
     2401 atttcttcag ccaaaacttc tgagattcgt ttatgcgtat cttgttctag tcctttactt
     2461 gcatgtataa tgattggctt agtattcatt ttaccaataa gttcctgtgc gactgaacga
     2521 atcgcttttg ttggaacaac aaataaaata gcatctgcgt tatccactgc ttctgccaat
     2581 tctttatatc cgataatcga ttctgggatc gtcaaatcag gtaaaaaatg ctgatttgta
     2641 tgatacttat tgatttcatc aatctgttca ggtacatttc cccaaatacg gacatcatgc
     2701 ccgttttctg ccaatgtttg tgctaaagca gtgccccaag atccaggacc aagaactgcg
     2761 attttttgtt tcatggatat tcctccaaat aaataattga taatttccat tatagtatac
     2821 atttcttaaa gttgggacta atttccattg tttttaagtg tttgtttact tcttt
//
LOCUS       DAZKPO010000154         2711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000154 DAZKPO010000000
VERSION     DAZKPO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.161"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG8NZ_002883"
     CDS             288..695
                     /locus_tag="ACG8NZ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540472.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG8NZ_002884"
     CDS             709..1110
                     /locus_tag="ACG8NZ_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540473.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACG8NZ_002885"
     CDS             1112..1483
                     /locus_tag="ACG8NZ_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540474.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="ACG8NZ_002886"
     CDS             complement(1519..1818)
                     /locus_tag="ACG8NZ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0540475.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="ACG8NZ_002887"
     CDS             complement(1824..2054)
                     /locus_tag="ACG8NZ_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540476.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(2313..2426)
                     /locus_tag="ACG8NZ_002888"
                     /pseudo
     CDS             complement(2313..2426)
                     /locus_tag="ACG8NZ_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          996 a          382 c          439 g          894 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcaca tcagtcacga tatcaaacaa
     1621 aaaatatacg tctttcttca ttctagtttt cgcagcaggg attttaaagt agttcttatt
     1681 agaatagtag agattaatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatc
     1741 ccacacctta taaaaatctt tgacaaatct attcgaagga tcaaatttaa accataattg
     1801 cgtctcatta aaaagcataa ccattactcc gatctatttt taaacttagt ctctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat ctggaatttg
     1981 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
     2041 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
     2101 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
     2161 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
     2221 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
     2281 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
     2341 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
     2401 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
     2461 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
     2521 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
     2581 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
     2641 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
     2701 atataaaaaa a
//
LOCUS       DAZKPO010000155         2542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000155 DAZKPO010000000
VERSION     DAZKPO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.162"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACG8NZ_002889"
     CDS             14..1168
                     /locus_tag="ACG8NZ_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0540477.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1141..1584
                     /locus_tag="ACG8NZ_002890"
     CDS             1141..1584
                     /locus_tag="ACG8NZ_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540478.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          892 a          431 c          440 g          779 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga at
//
LOCUS       DAZKPO010000156         2451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000156 DAZKPO010000000
VERSION     DAZKPO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.163"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..179
                     /locus_tag="ACG8NZ_002891"
     CDS             <1..179
                     /locus_tag="ACG8NZ_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0540479.1"
                     /translation="ILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEI
                     FLSYVQEAFYSSLI"
     gene            complement(1045..1575)
                     /locus_tag="ACG8NZ_002892"
     CDS             complement(1045..1575)
                     /locus_tag="ACG8NZ_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0540480.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1572..2189)
                     /locus_tag="ACG8NZ_002893"
     CDS             complement(1572..2189)
                     /locus_tag="ACG8NZ_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0540481.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          806 a          467 c          273 g          905 t
ORIGIN      
        1 ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg aatcagacat
       61 ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg aattacagaa
      121 caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc ttaatttgac
      181 aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt acctttctcc
      241 tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata ttcatcaaac
      301 tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt aatcttcttg
      361 ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga ttcttttctg
      421 ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact agtcctttgt
      481 tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac tccaagactt
      541 ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta ataaatatac
      601 attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct ttacaatcct
      661 aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct ttcttgatag
      721 tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt gctcaaaagt
      781 ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt tagggtcttg
      841 tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta tttggtttca
      901 ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg gagccccccc
      961 tataatctca ttattatttt catgactttt tactaccgtg gaattacaag caacaacaat
     1021 attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc aacaatgttt
     1081 acctataact attttattat ttttattcaa aatcctatga gtattcaatt caaaaatcgt
     1141 atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat ctttgaattg
     1201 aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg acccaatatc
     1261 taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg ctggcccttg
     1321 tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag tatttaatac
     1381 tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt tccctctcga
     1441 tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg catctgaaat
     1501 atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat tctttattat
     1561 caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt ataaaggatt
     1621 acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa cgttcctagc
     1681 ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat ttttcctcca
     1741 tatactattg aggaccatat tttgggtttc tttatatttt catatttaat agacattacc
     1801 atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga taactctttt
     1861 gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc agataaagga
     1921 tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt atagaatatc
     1981 aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag ttccattagt
     2041 atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa atagaccagt
     2101 cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata taatatatct
     2161 gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag tatacactta
     2221 tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta atattcaatt
     2281 tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt tttatacgat
     2341 tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt tttcatccac
     2401 ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc t
//
LOCUS       DAZKPO010000157         2419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000157 DAZKPO010000000
VERSION     DAZKPO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.164"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACG8NZ_002894"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACG8NZ_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACG8NZ_002895"
     CDS             1321..1779
                     /locus_tag="ACG8NZ_002895"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0540482.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACG8NZ_002896"
     CDS             1938..2408
                     /locus_tag="ACG8NZ_002896"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0540483.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          456 g          699 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DAZKPO010000158         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000158 DAZKPO010000000
VERSION     DAZKPO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.165"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACG8NZ_002897"
     CDS             complement(<1..103)
                     /locus_tag="ACG8NZ_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0540484.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACG8NZ_002898"
     CDS             complement(135..458)
                     /locus_tag="ACG8NZ_002898"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0540485.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACG8NZ_002899"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACG8NZ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DAZKPO010000159         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000159 DAZKPO010000000
VERSION     DAZKPO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.166"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8NZ_002900"
     CDS             207..2069
                     /locus_tag="ACG8NZ_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0540486.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8NZ_002901"
     CDS             2069..>2398
                     /locus_tag="ACG8NZ_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0540487.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKPO010000160         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000160 DAZKPO010000000
VERSION     DAZKPO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.167"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8NZ_002902"
     CDS             316..570
                     /locus_tag="ACG8NZ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0540488.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8NZ_002903"
     CDS             571..888
                     /locus_tag="ACG8NZ_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0540489.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8NZ_002904"
     CDS             1447..2382
                     /locus_tag="ACG8NZ_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540490.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKPO010000161         2320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000161 DAZKPO010000000
VERSION     DAZKPO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.168"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..386)
                     /locus_tag="ACG8NZ_002905"
     CDS             complement(90..386)
                     /locus_tag="ACG8NZ_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0540491.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            741..1097
                     /locus_tag="ACG8NZ_002906"
     CDS             741..1097
                     /locus_tag="ACG8NZ_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0540492.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1107..2006
                     /locus_tag="ACG8NZ_002907"
     CDS             1107..2006
                     /locus_tag="ACG8NZ_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0540493.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          767 a          387 c          439 g          727 t
ORIGIN      
        1 caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc ttcggtatta
       61 gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag cggttgatag
      121 ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg ttttagtaat
      181 tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa tccaaaacta
      241 ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt atcttcattg
      301 acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc ttcgataaaa
      361 tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt tatttgataa
      421 gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct ttttccaata
      481 gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag gaataaggtt
      541 aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag
      601 ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat
      661 aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc
      721 tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga
      781 agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat
      841 ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa
      901 aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa
      961 tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt gctttagaac
     1021 caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag
     1081 ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg
     1141 tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc
     1201 acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag
     1261 ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg
     1321 agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa
     1381 atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg
     1441 actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt
     1501 ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg
     1561 cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca
     1621 ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga
     1681 acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa
     1741 atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc
     1801 acacgattta cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt
     1861 ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc
     1921 gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac
     1981 aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt
     2041 gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt
     2101 tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta
     2161 gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt
     2221 tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcgag agtgtaaaat
     2281 attttgtgta aatgaaaaaa tccatacaaa aaaggaagtc
//
LOCUS       DAZKPO010000162         2279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000162 DAZKPO010000000
VERSION     DAZKPO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.169"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(149..448)
                     /locus_tag="ACG8NZ_002908"
     CDS             complement(149..448)
                     /locus_tag="ACG8NZ_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0540494.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            799..1431
                     /locus_tag="ACG8NZ_002909"
     CDS             799..1431
                     /locus_tag="ACG8NZ_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0540495.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1554..2192
                     /locus_tag="ACG8NZ_002910"
     CDS             1554..2192
                     /locus_tag="ACG8NZ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0540496.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          763 a          362 c          332 g          822 t
ORIGIN      
        1 tgttaattaa aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa
       61 aaatacagac tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt
      121 ttaattcatt gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag
      181 ccattcctcg acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg
      241 gtgatactgg tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat
      301 tatttatgac tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa
      361 gtatacgttc cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca
      421 tgttttctat ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc
      481 actacattct ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt
      541 gttaaaacct ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt
      601 aaatcagtaa aaatttttat tctaagattt ttattagaat tacgcctaat ttgtgtctct
      661 cagggcaatt ctaagaacgt ttcaagagat agcgacacta tcgttatcga aaaaggcgaa
      721 aacctatctc attttctgct aatgtaagtt ggtttagctt ttgttaatat gactttatga
      781 aaatggaggt aattaaatat gatttttcct aaagttaatg aagcaagctt gaataaagga
      841 tccttttttc atatctatga aaacgaagat tttgaactgt ataatatcct ttcacaaaaa
      901 gaggtcatcg aaaaccaaat aatctgccat ccaattattt acaaatctat tttcaagtat
      961 gcattttttg cttccaatga ttttcaaaga atggagatta ccgactgtat attcgaaaac
     1021 tgtgattttt caggctgtat attgacaaaa agcatgttgc atcgcgtgaa atttgtcaat
     1081 tgtaaattaa caggtactaa attgatggaa agttatattg ggaacacgtt attcgagaat
     1141 tgtaaaatgg actatgtaaa tttatctggt tcaaacataa aagaaagtac ctttgagcac
     1201 tctgtcttat caagcgcaga ttttgtagac tgttccctca ctaaaataat gttttctact
     1261 aatgatttag aaaatgctag cttttttgat actgatttaa aaaatatcga tttaagtagc
     1321 aactcctttg aaatgattga agtacgctcg gaacatctaa aaaattgtac gcttaatcag
     1381 ttgcaagcac ttggttttgc tagaaaattt ttacaaataa aagtagtata atcaaaatga
     1441 atatataaga taaattttta gcaatacaaa tatccataaa aaaacattgc ctattttaac
     1501 tcttcatgta tactgactct atcttcaaaa actgaatagt aggagttatt atcatgaata
     1561 acattttagg aaaaaaaata gaacttaaaa aagggatatt gggcactatt gtcgttgtac
     1621 tcggtttgtt agggttgcta tttgcgccat ccattattag cattttttct ttaactatca
     1681 ctggatttat agcatttaca cttgtttttg gtaaaaaaga tacgaaaaaa atgttctcga
     1741 aacctgtcgc tcctgtcaaa aatatagcca aatatttttt tatcaatgta gtaatcagtg
     1801 ccgcagtctc ggtatttttg caacaaattt tgaaatgggg tttaacaggc aatccaataa
     1861 atgaggcttt cagcccgtta ttatttatta ttttgcctat catgatactt ggagaagaat
     1921 tgttctcagt gtattttctc gctatttttt ctagtaagtt cagcattcct gtcgctagtt
     1981 tcttgtcagc aatcatcttt ggatttatcc attacagtac ttatgataat ggaaatattt
     2041 tacacactgt agcacacatc cttttgatac aaggggtggc aagattgtta ttcaaccaag
     2101 ctgccattaa aagcaactcg atcattacaa gttgggcagt tcatgtgata tttgatttca
     2161 cagctatatt gctagtcgtt ttattctcct aaatttttat agaaataata catgctctat
     2221 tgctaagaaa gcatacttta tttcctttaa acaaaaagac agatgtatca atggattaa
//
LOCUS       DAZKPO010000163         2259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000163 DAZKPO010000000
VERSION     DAZKPO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.170"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..>2259)
                     /locus_tag="ACG8NZ_002911"
     CDS             complement(148..>2259)
                     /locus_tag="ACG8NZ_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0540497.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DAZKPO010000164         2197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000164 DAZKPO010000000
VERSION     DAZKPO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.171"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..239)
                     /locus_tag="ACG8NZ_002912"
     CDS             complement(<1..239)
                     /locus_tag="ACG8NZ_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540498.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHN"
     gene            complement(241..1668)
                     /locus_tag="ACG8NZ_002913"
     CDS             complement(241..1668)
                     /locus_tag="ACG8NZ_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0540499.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1661..>2197)
                     /locus_tag="ACG8NZ_002914"
     CDS             complement(1661..>2197)
                     /locus_tag="ACG8NZ_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0540500.1"
                     /translation="HDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHG
                     SQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGP
                     DLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSD
                     KLLNRIDKEILKVGNIDE"
BASE COUNT          826 a          355 c          262 g          754 t
ORIGIN      
        1 tgattatggt tagttaacaa gttaatttta cgtttaaagg aagaaccgat tttccggtga
       61 ttcacaaaaa caacatcatt aaatagccct aattctttta tacgctcact atatcttcta
      121 gcgttttcaa attcagaata aataaataaa tctccttttg attctcggta aaaattttga
      181 taaacattta atatattaat caaatgtaaa ggagtccaag caatgaaagc tgtcttcata
      241 ttaattctcc ttttttaaat acaagtaaga aatgcttccc aaataatacc agaaaacaat
      301 tgaaatgaca tcgcgcttat tgaaaagtac atgagcctcc accatttcat ctataaaaat
      361 agatagtata aaaattaaaa caataccaag aaacgcataa ttcactttac ttctaaaaaa
      421 tgttttgagt aaactaaaga tatttaacac atagaaagca tagatacata taaaagcaat
      481 tataccactt gatacaagta cttgaagaaa tccattatgc atgtttccat gtgctctttt
      541 tagttcattc acatctgtag tatcaagatc tacttttttc aaaaattttg catcgctatt
      601 cccatagaaa tcggtctctc cataaccaaa taacaaagtt ggtcttataa ctttaagcgc
      661 agctttccaa atcgtaaaac gaccagctga aatttcggag ttatcactag aatgctgtaa
      721 tattacttct ttttctggaa gtggagtttt atctgattca tttgactcta taacttctac
      781 ttttctatca atcgatacag gaattgttgc taaaaaatcc ttagcataat tagtaccaaa
      841 agaaaaatat accaatagaa ctaataatat gcttaccatc catttgattt ttacaggtag
      901 tacttctcta atgctctttc taaattttga attggcaaac aacaaaagaa ataatactaa
      961 aaaagaacaa attactacct gggcacctct agacgaagaa agtgtaatat aaaatatttg
     1021 tacgataaaa ttcatcatat aaaaaacacg aatcgttttt gttatttttg aaattaatac
     1081 actaattaaa cttaaaataa tagaagtata tccaaacata gctccaacat ttggacttgt
     1141 atagacacca aataaccgat gttcaataaa accttgtctt gcaagtaatc ctggcgttcc
     1201 tgttgggaca tgataagaaa tcaaaaaaat aaattgatag atagatatta gcgcagctaa
     1261 aaaaattatg ataatgaaaa cattattaaa atacaccatt tttttctttt tttcctcttc
     1321 actttgtgaa aagtctgcag gaaaaattac tatcaaagta atcagcaaat atccaaaatt
     1381 ataaatagat ggaattaaaa aattttctct gttaatgatt atagttaaga taaaactaat
     1441 cattgctcca atcagtagcc aagaaaaacg cattttaaaa aaatttttct ttataaaaac
     1501 ctcatacaaa actataattc caccccaagc aaaagctatt ttcatcggga tattagttac
     1561 attaaaagta ctaggcataa aataaagcag acaaatgatt aaatttatta ctcgaaacgc
     1621 atctcgactt aataaactct tctctaaaac aggtactatt ctattcatct atatttccaa
     1681 ctttcaaaat ttctttatca attctattta atagtttatc agagtaatcc gatttaactt
     1741 ttcttacaaa taatttttca ctttctttta tttgatctat atccctttca tcaaaccatt
     1801 tgttcatgga aggatcaata atatgaatat ttgctaattt cgaagtttgt aaagtagttt
     1861 cttcaatatt tttcaattca agtggtaaat ctggtcctcc aataacattt ttatttttga
     1921 acgaggaatt aaaaaaaagt gtatgaaaaa atttttcatc tggggcaaac atacctattt
     1981 taaaattcag gtagtcttgt tgattttgtt catacatttg tattaattct gttaggcatt
     2041 ctttcgacaa agcccaccat tgagaaccat gatacaaatc aaatttatgg tgtctaattt
     2101 ttgggggaag tttaatcatc ctcagacaac gattaattat agctctaaaa attttaaatg
     2161 cagttgtgtt attcctatta attctaggat aatcatg
//
LOCUS       DAZKPO010000165         2177 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000165 DAZKPO010000000
VERSION     DAZKPO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.172"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8NZ_002915"
     CDS             complement(<1..914)
                     /locus_tag="ACG8NZ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0540501.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8NZ_002916"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8NZ_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0540502.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG8NZ_002917"
     CDS             2006..2176
                     /locus_tag="ACG8NZ_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540503.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          740 a          384 c          416 g          637 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaa
//
LOCUS       DAZKPO010000166         2119 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000166 DAZKPO010000000
VERSION     DAZKPO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.173"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            732..1064
                     /gene="traE"
                     /locus_tag="ACG8NZ_002918"
     CDS             732..1064
                     /gene="traE"
                     /locus_tag="ACG8NZ_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0540504.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1683..2024
                     /locus_tag="ACG8NZ_002919"
     CDS             1683..2024
                     /locus_tag="ACG8NZ_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0540505.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          746 a          279 c          438 g          656 t
ORIGIN      
        1 aatagctatt ttaatagaat ttataagtta aaatattatg ataaaaatca caaacgtagc
       61 cttttacgtc ataagtgtta aaagaaataa ttaatttcaa atatagaaaa gggaacgtat
      121 gtttgcatac tccttttttt tgagttataa taatacgcga agagagataa tttcattatt
      181 taaacaagct aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta
      241 cattttttgt ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca
      301 cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc
      361 ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg
      421 ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag
      481 tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat
      541 gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa
      601 attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc
      661 aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg
      721 tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta
      781 aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg
      841 ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt
      901 acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc
      961 caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag
     1021 tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata
     1081 acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt
     1141 agagtcacta gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga
     1201 cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt
     1261 ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt
     1321 tagaagttag acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct
     1381 agactttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac
     1441 aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga
     1501 tcaatcgagc atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc
     1561 gaaatttttt atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat
     1621 aaagtgaaaa aattaatata aagcatagag aaaatgaaat ttacgtaatg gaggaatgac
     1681 aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc
     1741 aacgttggga ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag
     1801 atgttttagc attagacgag gatgatttat acgatcaaac attgactgat aatccaattg
     1861 gattcggctt gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga
     1921 atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg
     1981 tcagctttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa
     2041 ttttacagat aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta
     2101 ggcattgagt aaggaattt
//
LOCUS       DAZKPO010000167         2065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000167 DAZKPO010000000
VERSION     DAZKPO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.174"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8NZ_002920"
     CDS             <1..156
                     /locus_tag="ACG8NZ_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0540506.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8NZ_002921"
     CDS             169..1077
                     /locus_tag="ACG8NZ_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0540507.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="ACG8NZ_002922"
     CDS             1077..1694
                     /locus_tag="ACG8NZ_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540508.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1698..>2065
                     /locus_tag="ACG8NZ_002923"
     CDS             1698..>2065
                     /locus_tag="ACG8NZ_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540509.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          745 a          298 c          444 g          578 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcggt gtacaggttt
     1741 atccacaaac tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag
     1801 gcgatcctgg ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt
     1861 ctgttacgaa tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag
     1921 ataaaggtga tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg
     1981 ggttgatgtc gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa
     2041 atccaggtaa tgctacaact acgac
//
LOCUS       DAZKPO010000168         2019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000168 DAZKPO010000000
VERSION     DAZKPO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.175"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG8NZ_002924"
     CDS             100..570
                     /locus_tag="ACG8NZ_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0540510.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG8NZ_002925"
     CDS             792..1895
                     /locus_tag="ACG8NZ_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0540511.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKPO010000169         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000169 DAZKPO010000000
VERSION     DAZKPO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.176"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8NZ_002926"
     CDS             <1..378
                     /locus_tag="ACG8NZ_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0540512.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8NZ_002927"
     CDS             426..1826
                     /locus_tag="ACG8NZ_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0540513.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKPO010000170         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000170 DAZKPO010000000
VERSION     DAZKPO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.177"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8NZ_002928"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8NZ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8NZ_002929"
     CDS             complement(728..1744)
                     /locus_tag="ACG8NZ_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0540514.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKPO010000171         1945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000171 DAZKPO010000000
VERSION     DAZKPO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.178"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8NZ_002930"
     CDS             <1..156
                     /locus_tag="ACG8NZ_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0540515.1"
                     /translation="FEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEV
                     LKIAEEK"
     gene            157..909
                     /locus_tag="ACG8NZ_002931"
     CDS             157..909
                     /locus_tag="ACG8NZ_002931"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0540516.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            914..1861
                     /locus_tag="ACG8NZ_002932"
     CDS             914..1861
                     /locus_tag="ACG8NZ_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0540517.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          677 a          263 c          388 g          617 t
ORIGIN      
        1 tttgaagaaa acattgttaa agaaatgcct gaatctgcta atgatatgta tgagaaggag
       61 ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga tttatatcat
      121 gcaaacgaag tattgaaaat agctgaggag aaataaatgg aaattttatt tactatttgt
      181 ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga aatatttggc aggaaagccc
      241 ctagtccatt tcgctttagc aatgatagat atctatcaac aaaaacatcc tgaacataat
      301 tgcacgactt gtttgaatac ggacagtaat gagttagtag aattgacgac taagttaaat
      361 ccgtctgtga aaattgtaga acgtaaacct gaattagcta cagatactgt agggaaaatt
      421 gaagtgatac gtgattcatt cttacaacta gaaagagaag ataatatatt tgatttaatt
      481 atagatttag atttgacttc acctttacgt gaagtttcta atcttgaaga attaatagat
      541 gaacatctaa aaaatttaga taaagacgtc tacttttcag ttgtttctgc aagacgtaat
      601 ccctatttta atatggtaaa aagaattgaa aatgaagtta ctatagttaa taaatcaaga
      661 tacacatcta ggcaacagac acctgaagtt tttgaaatga atgcctcaat gtattctttt
      721 aaacctgaat ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg gataataaaa
      781 atgagagatt atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt
      841 ttgtttccta aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat
      901 cggaggtaaa aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac
      961 cttagccaat catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg
     1021 aaaagtagaa aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga
     1081 gaaatccttg atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc
     1141 agcagttgct agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt
     1201 tgaaagtgta ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat
     1261 cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga
     1321 gtccgtgatt cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt
     1381 gctagattat tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta
     1441 tggtccaaat caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg
     1501 ctataagaaa caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca
     1561 gtctagagac tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga
     1621 ggaagctgca ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga
     1681 attgattcat gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga
     1741 gcgttcgggt gatatccatg actctctagc agatattact aagatccagt caattggcta
     1801 tcaaccgaaa tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta
     1861 aaacatacta agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga
     1921 gttttgtaaa atcaaatctg ggata
//
LOCUS       DAZKPO010000172         1793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000172 DAZKPO010000000
VERSION     DAZKPO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.180"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..613
                     /locus_tag="ACG8NZ_002933"
     CDS             <1..613
                     /locus_tag="ACG8NZ_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540518.1"
                     /translation="KKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIE
                     EVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKN
                     ILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALS
                     EDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            600..>1793
                     /locus_tag="ACG8NZ_002934"
     CDS             600..>1793
                     /locus_tag="ACG8NZ_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0540519.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFL"
BASE COUNT          594 a          230 c          286 g          683 t
ORIGIN      
        1 aaaaaaaatt gacgattatt ttgtctattg tccggaattg ttactttatg atgtaaaaaa
       61 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
      121 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      181 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      241 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      301 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      361 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      421 aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata cagcattatc
      481 tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct
      541 atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta
      601 tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc tggtatacta
      661 tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt gtaaatatta
      721 ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca attttttcga
      781 ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac tataaagatg
      841 acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta attgtgttct
      901 tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg aaccaacttg
      961 tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat atccagtctt
     1021 attttacgat taactttaac cattttaaat atttgatttt atcttttata tctacttttt
     1081 tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg
     1141 gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa
     1201 taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct
     1261 ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta
     1321 tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag
     1381 gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg
     1441 gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag
     1501 tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg
     1561 ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta ggctatttct
     1621 ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta
     1681 ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg attttcatac
     1741 ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt tta
//
LOCUS       DAZKPO010000173         1762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000173 DAZKPO010000000
VERSION     DAZKPO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.181"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      198..364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            538..1644
                     /locus_tag="ACG8NZ_002935"
     CDS             538..1644
                     /locus_tag="ACG8NZ_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0540520.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          522 a          284 c          360 g          596 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg
      541 atcggtcttt tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat
      601 acaaaaagtc tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc
      661 ttagctcgtt cagtcaaact acactattta cttcacccta aaatagcagg atacggttta
      721 gccttatcga ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac
      781 ggcgcacaac gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat
      841 ctttttttga ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat
      901 ctaaaaaaac cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag
      961 attgctggag cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt
     1021 ccatttaaaa aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca
     1081 ggcggggtat tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat
     1141 gaaaggatcg cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg
     1201 acccattcgt tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt
     1261 atcacaaaaa aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca
     1321 gaagagcttg ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg
     1381 agaatctttt gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga
     1441 ttcggaactt tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg
     1501 ccaatgactg gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt
     1561 tctttaggaa tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg
     1621 ctctatcgac ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata
     1681 aagtacaatc ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa
     1741 agtatctcaa aaaatcatat ta
//
LOCUS       DAZKPO010000174         1731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000174 DAZKPO010000000
VERSION     DAZKPO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.182"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8NZ_002936"
     CDS             <1..156
                     /locus_tag="ACG8NZ_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0540521.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8NZ_002937"
     CDS             169..1077
                     /locus_tag="ACG8NZ_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0540522.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="ACG8NZ_002938"
     CDS             1077..1697
                     /locus_tag="ACG8NZ_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540523.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          613 a          251 c          395 g          472 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DAZKPO010000175         1728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000175 DAZKPO010000000
VERSION     DAZKPO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.183"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..1121)
                     /locus_tag="ACG8NZ_002939"
     CDS             complement(102..1121)
                     /locus_tag="ACG8NZ_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0540524.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1118..1342)
                     /locus_tag="ACG8NZ_002940"
     CDS             complement(1118..1342)
                     /locus_tag="ACG8NZ_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0540525.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1339..1632)
                     /locus_tag="ACG8NZ_002941"
     CDS             complement(1339..1632)
                     /locus_tag="ACG8NZ_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540526.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          485 a          354 c          291 g          598 t
ORIGIN      
        1 tatgggtgat agtgaagata tgcgataaca tcactatttt atcgaatgat ttttatagtt
       61 gtcaatatag ttatgtaatg ctcctcaacg aggagctatt tttatcgttt aggaatattt
      121 aaataccaac gtttgtcatg gaaatcttgc gcaccgcctt tagtgtttcc ctctggatca
      181 ttcgttgccc gcatcatgac atagactttc ttattaggga agttacgcat gttaaaagat
      241 acatgatagc caacatttcc agaagtatta taagcttgat ttacatctga tctataaatt
      301 ccatcagctc ttactcgagc taattctttc ccagtattgt aatccataat gaagatatac
      361 tcgtatttat agttagcaat gtgccatcca gctacatgca aatttgcatt ttcgatttct
      421 ccaaactgat caatgtgggc gtaattcgtt ccatctgtca gtgtaggatt agctgcacca
      481 gctcgtgttg gatcaatgac aggcttgttt tcagaagttg ttggattttc atcggtaaat
      541 ccatgagcta aatcatatgc taatttttct ttacttacgc ccatttcaga aagataaccg
      601 taaggatctg tatgatcgcc ccagatgttt tgtgttaccc ataaatgcga tttgattccc
      661 ggttggttat aaggagtgtc caacgttaat ggaataccat atttcattgc tgaatctcta
      721 gccaattcaa cgtatgcctt gtagtttttc tcaaacgttg ctttatcatg tgtgtgttgt
      781 aactcaatct gcacaggact gttggcatta gcatacgaac cagcaccgta ctgtacataa
      841 ccaggttgac cgacttgata aacaattccg ccgtctccca caatataagc agtataagcg
      901 ctagtccatg aacgttgcat atactgcgct tcattgcgtc ctgttgctgt ttcattagcc
      961 gtttcatgca gtaaaatata ctgattattt gctacttgtg agctaccttc atttgcgccc
     1021 aaattaaatt cattgttgat agtataggca aatccgttaa ttggcaataa aaaaagagcc
     1081 gttaacaggc tcatcgcagt aatagtaatt ttctttttca tttgtttcct ccttcttcgc
     1141 tttcagccga gaacattttg taggttcgat ttgatacacc caacacactc cctaaaaacg
     1201 cgccaaaacc agtaatgatg acaacacaga tatctgtgta ctgccaattg agcgctttac
     1261 caactaaccc cacgaaagta gctagtgcgg gaataattac cagtgcgaac cattttagta
     1321 cttcgaacgt tttattattc attttcttct ctccctaaac aaagttttga tttgttgcgt
     1381 gtgttccacc aatttttctg tatgtgtatc taatctttca tcgtgtttct ttagttcttc
     1441 atgaatcatc aatcgatctg atttgctcga ttctaaatct ttagtcagca aatctaaatt
     1501 gtggcttact tttgaaagag tctcagtaat tttcgagaaa gatgcagcaa tcggtctaat
     1561 tactaataaa atcaaagaaa cgatagcggt tattgatcct gctatcattc cccattcccc
     1621 taaattaatc atgtgacaac tcctttacct taaataaaaa cgcatcaatt aagatgcgct
     1681 cttatcttta ttaatgattt tatctgcttc ttcgtctgta atgcataa
//
LOCUS       DAZKPO010000176         1636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000176 DAZKPO010000000
VERSION     DAZKPO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.184"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1502)
                     /locus_tag="ACG8NZ_002942"
     CDS             complement(183..1502)
                     /locus_tag="ACG8NZ_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0540527.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAZKPO010000177         1564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000177 DAZKPO010000000
VERSION     DAZKPO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.185"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8NZ_002943"
     CDS             131..505
                     /locus_tag="ACG8NZ_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0540528.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8NZ_002944"
     CDS             complement(619..1248)
                     /locus_tag="ACG8NZ_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0540529.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8NZ_002945"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8NZ_002945"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          538 a          232 c          271 g          523 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaa
//
LOCUS       DAZKPO010000178         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000178 DAZKPO010000000
VERSION     DAZKPO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.186"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8NZ_002946"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8NZ_002946"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKPO010000179         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000179 DAZKPO010000000
VERSION     DAZKPO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.187"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8NZ_002947"
     CDS             complement(150..1337)
                     /locus_tag="ACG8NZ_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0540530.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKPO010000180         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000180 DAZKPO010000000
VERSION     DAZKPO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.188"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8NZ_002948"
     CDS             82..>1364
                     /locus_tag="ACG8NZ_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0540531.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKPO010000181         1359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000181 DAZKPO010000000
VERSION     DAZKPO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.189"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG8NZ_002949"
     CDS             complement(<1..208)
                     /locus_tag="ACG8NZ_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540532.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="ACG8NZ_002950"
     CDS             complement(305..757)
                     /locus_tag="ACG8NZ_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540533.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..1312)
                     /locus_tag="ACG8NZ_002951"
     CDS             complement(764..1312)
                     /locus_tag="ACG8NZ_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0540534.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          487 a          264 c          132 g          476 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
     1261 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1321 ctccataaaa aataagttac tttttaaata tcttgttta
//
LOCUS       DAZKPO010000182         1354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000182 DAZKPO010000000
VERSION     DAZKPO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.190"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            553..1086
                     /locus_tag="ACG8NZ_002952"
     CDS             553..1086
                     /locus_tag="ACG8NZ_002952"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0540535.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          430 a          249 c          254 g          421 t
ORIGIN      
        1 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
       61 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
      121 tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattga
      181 gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca aaatagttcc
      241 tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg
      301 gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg aaaatggttc
      361 ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca tctaactcca
      421 aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat aaaaaattct
      481 atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat ttcccaagga
      541 ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca tcagccttat
      601 agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga tgaattctat
      661 acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca cggaatccat
      721 caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat ccaaccatat
      781 tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg
      841 ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg tacagtgaaa
      901 agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat
      961 ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc atgtgcggaa
     1021 atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact tgtgactata
     1081 aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt tcactccgag
     1141 tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt gactggtgac
     1201 cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct gttctgcatc
     1261 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttggacttat
     1321 taccgcagat gcaagctttt ggacaccgtt tacc
//
LOCUS       DAZKPO010000183         1324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000183 DAZKPO010000000
VERSION     DAZKPO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.191"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8NZ_002953"
     CDS             278..820
                     /locus_tag="ACG8NZ_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540536.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACG8NZ_002954"
     CDS             976..>1324
                     /locus_tag="ACG8NZ_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0540537.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAZKPO010000184         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000184 DAZKPO010000000
VERSION     DAZKPO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.192"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8NZ_002955"
     CDS             <1..1244
                     /locus_tag="ACG8NZ_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0540538.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKPO010000185         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000185 DAZKPO010000000
VERSION     DAZKPO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.193"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8NZ_002956"
     CDS             <1..1076
                     /locus_tag="ACG8NZ_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0540539.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZKPO010000186         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000186 DAZKPO010000000
VERSION     DAZKPO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.194"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..499)
                     /locus_tag="ACG8NZ_002957"
     CDS             complement(<1..499)
                     /locus_tag="ACG8NZ_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0540540.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGG"
     gene            complement(499..>1170)
                     /locus_tag="ACG8NZ_002958"
     CDS             complement(499..>1170)
                     /locus_tag="ACG8NZ_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0540541.1"
                     /translation="IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQ
                     YLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDID
                     YSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETS
                     SMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLIN
                     DIEVN"
BASE COUNT          396 a          203 c          131 g          440 t
ORIGIN      
        1 ctcctccccc catagattta aaggcagaat aattatttct ataatcagta ccttttctcc
       61 aatcaggaag ataagtagaa caaatagctc ttacagaata tactttttta ctttgaatac
      121 tattacgaac aacttccatg atatttttat acctcagtgg agcagcaaca taaatatttt
      181 tgtaatcata attatattga gtaagatcaa tactctcaga aaaagcaggt ttttctaaaa
      241 atatgtaatc aactttttca acaattacat ttagtgtttc cagatgctta aaagtcggat
      301 ttgtaataaa agctatatca taatgtgtat ccaaatcgtc tctattttga atatacgtta
      361 tttcttcaaa ctcttttttt ccactgctac tataagctgt tatatcaaaa ttaatagaat
      421 tcttttttcc atagcttatt agatttttta gatgtctctt tccaattgac cctaacccaa
      481 aaaaaattac tttcatcatt aatttacctc aatgtcgtta attaagttaa taatatactt
      541 atctttttca aaagtatatt ggggttccat tttcttttcc aaaagcgtga aaaattcttt
      601 aatctcctct aaatatgcat cttcaataat agtatgtgaa taattattat catgcataac
      661 ctcatcgtac aattgaatat ctgtcatact agaagtttct tcattccatg aaaatagaga
      721 atccgggtta ccttcccatt ctattaaaat cttttcacca attatagtta aatttctttt
      781 agctactctt gaaacaacat ctacacttaa agttccaact ataccagttt catgctcaat
      841 aataagagtg aaactgtccg aataatcaat atcaagattg gataatttct gggtcattac
      901 agtaaacgat ttaactttcc caaatgtatc tataatccaa ggtaattcaa tagcgaatat
      961 ttctctacac ccattagttt ttttgtttga aacaaaaaaa ttgcgataat cttcccaagg
     1021 atgccagtcg ggtaaatact gtcctacatg gtattgataa gaaatttttt tattatgcgt
     1081 cactttagac ttaatatatt gaatttcttt tctcttcaaa aatgtagaag atagataaag
     1141 taatctatct ttctttttag ctacttcgat
//
LOCUS       DAZKPO010000187         1151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000187 DAZKPO010000000
VERSION     DAZKPO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.195"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8NZ_002959"
     CDS             154..417
                     /locus_tag="ACG8NZ_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0540542.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="ACG8NZ_002960"
     CDS             417..689
                     /locus_tag="ACG8NZ_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0540543.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="ACG8NZ_002961"
     CDS             766..1095
                     /locus_tag="ACG8NZ_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0540544.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1085..>1151
                     /locus_tag="ACG8NZ_002962"
     CDS             1085..>1151
                     /locus_tag="ACG8NZ_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0540545.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKP"
BASE COUNT          442 a          145 c          226 g          338 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa acatgtcatt aagtatttaa aaaagctaaa
     1141 agagaagcca t
//
LOCUS       DAZKPO010000188         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000188 DAZKPO010000000
VERSION     DAZKPO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.197"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8NZ_002963"
     CDS             <1..355
                     /locus_tag="ACG8NZ_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540546.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8NZ_002964"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8NZ_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACG8NZ_002965"
     CDS             985..>1137
                     /locus_tag="ACG8NZ_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0540547.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAZKPO010000189         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000189 DAZKPO010000000
VERSION     DAZKPO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.198"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8NZ_002966"
     rRNA            <1..>1073
                     /locus_tag="ACG8NZ_002966"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKPO010000190         1069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000190 DAZKPO010000000
VERSION     DAZKPO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.199"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8NZ_002967"
     CDS             <1..969
                     /locus_tag="ACG8NZ_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0540548.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZKPO010000191         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000191 DAZKPO010000000
VERSION     DAZKPO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.200"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8NZ_002968"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8NZ_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0540549.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DAZKPO010000192         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000192 DAZKPO010000000
VERSION     DAZKPO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.201"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..190)
                     /locus_tag="ACG8NZ_002969"
     tRNA            complement(117..190)
                     /locus_tag="ACG8NZ_002969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Ile,seq:cat)
     gene            complement(745..1059)
                     /locus_tag="ACG8NZ_002970"
     CDS             complement(745..1059)
                     /locus_tag="ACG8NZ_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540550.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          399 a          168 c          166 g          334 t
ORIGIN      
        1 tccataaaag gatacataaa aaagccactc atttgagtgg caatgaagaa aagctttagc
       61 ttgtataata ctcttcaaaa aattctaaca cagaacgatt caattggcta cattaatgta
      121 ccctgtagga ctcgaaccta cgaccggacg gttatgagcc gtctgctctg accaactgag
      181 ctaagggtag tggttgttgc cacataaagc cataaacaat caaccagtag aatgcgtggc
      241 aacaaacctg ttatcgcata tcttggagtg tgactattta tgagtgatag tgaagatatg
      301 cgtctccata tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa
      361 cgttttttta ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat
      421 tttgcattca aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct
      481 attttaaggt ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg
      541 ctgatggaag gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat
      601 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
      661 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      721 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      781 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      841 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      901 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      961 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
     1021 tattgctatt attaagataa aattactgta aaaatccaaa agctatc
//
LOCUS       DAZKPO010000193         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000193 DAZKPO010000000
VERSION     DAZKPO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.202"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8NZ_002971"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8NZ_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0540551.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZKPO010000194         1057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000194 DAZKPO010000000
VERSION     DAZKPO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.203"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG8NZ_002972"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG8NZ_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          370 a          192 c          213 g          282 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaata
//
LOCUS       DAZKPO010000195         1002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000195 DAZKPO010000000
VERSION     DAZKPO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.204"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1002
                     /locus_tag="ACG8NZ_002973"
                     /pseudo
     CDS             369..1002
                     /locus_tag="ACG8NZ_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAZKPO010000196          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000196 DAZKPO010000000
VERSION     DAZKPO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.205"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8NZ_002974"
     CDS             <1..459
                     /locus_tag="ACG8NZ_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540552.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8NZ_002975"
     CDS             473..904
                     /locus_tag="ACG8NZ_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540553.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAZKPO010000197          957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000197 DAZKPO010000000
VERSION     DAZKPO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.206"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8NZ_002976"
     CDS             <1..878
                     /locus_tag="ACG8NZ_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0540554.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          300 a          212 c          191 g          254 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttt
//
LOCUS       DAZKPO010000198          921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000198 DAZKPO010000000
VERSION     DAZKPO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.208"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>921
                     /gene="bsh"
                     /locus_tag="ACG8NZ_002977"
     CDS             4..>921
                     /gene="bsh"
                     /locus_tag="ACG8NZ_002977"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0540555.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDS"
BASE COUNT          315 a          123 c          169 g          314 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag t
//
LOCUS       DAZKPO010000199          908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000199 DAZKPO010000000
VERSION     DAZKPO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.209"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>908
                     /locus_tag="ACG8NZ_002978"
     CDS             <1..>908
                     /locus_tag="ACG8NZ_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540556.1"
                     /translation="RWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFH
                     INRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYI
                     VTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLI
                     LLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFL
                     IIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSE
                     LLSFGIGLTLLICLIILTFNFKKLGI"
BASE COUNT          289 a          118 c          132 g          369 t
ORIGIN      
        1 gaggtggtat atgaatttga aaaaaatttt ttctgtaaaa atatttatag tttatgcatt
       61 tgttagttta ttatatttat ttccagcatt taaaaacatg gaaatatttg gcttttatga
      121 tacggcattt cacattaatc gtgcgttaag tttagaatct atttttagct cccctattaa
      181 ttttgaaacg tttagatcat atggcatgca agtaaataat ttttacccat ggttaactct
      241 atatccttta tttttattga tcaagtttac taatttagca ataggctata acctttttct
      301 ttatattgtt acgctcatta ctctatttat atgtcattat gtaatgtatg aaattaccaa
      361 aaaacatgtt actagctcct ttttcgctat tatatataca acatcttctt ttaggagtgt
      421 agaaatattt cttcgagggg caatggggga attactcgca atgtcaatac ttcctcttat
      481 tttactcggg tttataaagc tttacgactc aaaaaaagaa tcttgggtta tgttggcgat
      541 tagcatgact ttattaattt acactcatgt tctttctgta gctatgacta tggtaatgat
      601 tataattgct ttaattattc aattttatcg gaaaaagaaa atagaaatct tcttaattat
      661 taaattaata aaagctgcta tagttacttt acttctttca ctagcgttta taggtcctat
      721 gattgagcag acattgtatc aagatctgaa tagaccttat gtggatatat tacaaaaaag
      781 agcaatttat ctgggtaaag agtttttgat tggaagtata gattcagaat tactatcatt
      841 tggtattggc ttaactttgc ttatttgttt aattatacta acatttaact ttaaaaaatt
      901 aggaatct
//
LOCUS       DAZKPO010000200          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000200 DAZKPO010000000
VERSION     DAZKPO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.210"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG8NZ_002979"
     CDS             256..879
                     /locus_tag="ACG8NZ_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540557.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaagaa
//
LOCUS       DAZKPO010000201          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000201 DAZKPO010000000
VERSION     DAZKPO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.212"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG8NZ_002980"
     CDS             complement(<1..153)
                     /locus_tag="ACG8NZ_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0540558.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            231..809
                     /locus_tag="ACG8NZ_002981"
                     /pseudo
     CDS             231..809
                     /locus_tag="ACG8NZ_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          259 a          151 c          158 g          254 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DAZKPO010000202          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000202 DAZKPO010000000
VERSION     DAZKPO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.213"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8NZ_002982"
     CDS             <1..672
                     /locus_tag="ACG8NZ_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0540559.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKPO010000203          768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000203 DAZKPO010000000
VERSION     DAZKPO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.214"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..732
                     /locus_tag="ACG8NZ_002983"
     CDS             19..732
                     /locus_tag="ACG8NZ_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540560.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT          254 a          150 c          137 g          227 t
ORIGIN      
        1 aataatgagg tattttatat gaaaaagtta ggttttattt tattgttggt ctctttattc
       61 cttgtaggat gtacgtctcc tagtaaagaa aatagtgatc catctgcgac cacagacaaa
      121 agttcaatca gacaaaccag ttcagctgaa aaatcaagta ctcagaaatc agcttctact
      181 gaaacgaata catcttcaac aagcagtaaa gtctcagagc ccaatacttc ttctacatct
      241 agtagtacag ttgagacaaa tatttcttct tcaacaatag acacaacctc ctcacagtct
      301 tctcagtcaa tagatccgtt agcaggatat tctgatctac aaattgagta cgcacgtgtt
      361 tggctagcta ttagaggtaa tacttttaga gatacatcgg gtgatttcga attacatgtt
      421 attcattatc ctgcaggaac acctatcgcc ccttatgatg cagggagtgc agtctatccg
      481 aaagatgtgg ttatgctcac tgggaaatac agttaccagg gagttttagt gtattcaagt
      541 aaccatgacg ggacaatcac tagttatcca gttccttcac attggcaaat gcctgcagat
      601 caaacatctt atccagcgtt cattagagaa acaactcaag aaatcattga taatgcatct
      661 gtagtagcta ttcctacagg aaatcccaat gacgtgaaac agttaatcga tgtgacagtt
      721 atagataatt agactttcta atatgttata caaaaagaag ctgacagc
//
LOCUS       DAZKPO010000204          764 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000204 DAZKPO010000000
VERSION     DAZKPO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.215"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..106)
                     /locus_tag="ACG8NZ_002984"
     tRNA            complement(34..106)
                     /locus_tag="ACG8NZ_002984"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:His,seq:gtg)
     gene            complement(109..182)
                     /locus_tag="ACG8NZ_002985"
     tRNA            complement(109..182)
                     /locus_tag="ACG8NZ_002985"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Trp,seq:cca)
     gene            complement(189..269)
                     /locus_tag="ACG8NZ_002986"
     tRNA            complement(189..269)
                     /locus_tag="ACG8NZ_002986"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Tyr,seq:gta)
     gene            complement(271..346)
                     /locus_tag="ACG8NZ_002987"
     tRNA            complement(271..346)
                     /locus_tag="ACG8NZ_002987"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Phe,seq:gaa)
     gene            complement(382..454)
                     /locus_tag="ACG8NZ_002988"
     tRNA            complement(382..454)
                     /locus_tag="ACG8NZ_002988"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(419..421),aa:Asp,seq:gtc)
     gene            complement(464..536)
                     /locus_tag="ACG8NZ_002989"
     tRNA            complement(464..536)
                     /locus_tag="ACG8NZ_002989"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Val,seq:tac)
     gene            complement(552..623)
                     /locus_tag="ACG8NZ_002990"
     tRNA            complement(552..623)
                     /locus_tag="ACG8NZ_002990"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Glu,seq:ttc)
     gene            complement(638..727)
                     /locus_tag="ACG8NZ_002991"
     tRNA            complement(638..727)
                     /locus_tag="ACG8NZ_002991"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Ser,seq:gga)
BASE COUNT          210 a          210 c          176 g          168 t
ORIGIN      
        1 cttaccgctt ggctataccc caagaataaa aagggcgaat gatgggaatc gaacccacga
       61 atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatg gcaggggcag
      121 caggaattga acccacacta acggttttgg agaccgctgt tctaccttta aactatgccc
      181 ctataaaatg gaggagagtg gattcgaacc actgaaccct aaggaacgga tttacagtcc
      241 gtcgcgttta gccacttcgc tactcctcca tggtggcgcg ggacagaatc gaactgccga
      301 cacacggagc ttcaatccgt tgctctacca actgagctac cgcgccatat tttaaaatta
      361 ttaattgaat aacaagaacg acggttccga cgggaatcga acccgcgatc tcctgcgtga
      421 caggcaggca tgttaacccc tacaccacgg aaccagttca ttatggaggt taacgggatc
      481 gaaccgctga ccctctgctt gtaaggcaga tgctctccca gctgagctaa acctccaaca
      541 ttcaattaaa atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt
      601 cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg attcgaaccc
      661 ttgagacagt ttggcaccat ctacatgatt tccaatcatg ctccttcggc ctctcggaca
      721 actctccaga gtgaaagaag ctcaatactc tttctcaaaa tgag
//
LOCUS       DAZKPO010000205          754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000205 DAZKPO010000000
VERSION     DAZKPO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.216"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..>754)
                     /locus_tag="ACG8NZ_002992"
     CDS             complement(91..>754)
                     /locus_tag="ACG8NZ_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0540561.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          204 a          177 c          145 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgc
//
LOCUS       DAZKPO010000206          750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000206 DAZKPO010000000
VERSION     DAZKPO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.217"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8NZ_002993"
     CDS             132..344
                     /locus_tag="ACG8NZ_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540562.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>750
                     /locus_tag="ACG8NZ_002994"
     CDS             506..>750
                     /locus_tag="ACG8NZ_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0540563.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDI"
BASE COUNT          277 a          105 c          127 g          241 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa
//
LOCUS       DAZKPO010000207          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000207 DAZKPO010000000
VERSION     DAZKPO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.218"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8NZ_002995"
     CDS             <1..>725
                     /locus_tag="ACG8NZ_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0540564.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKPO010000208          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000208 DAZKPO010000000
VERSION     DAZKPO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.219"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..>719)
                     /locus_tag="ACG8NZ_002996"
     CDS             complement(334..>719)
                     /locus_tag="ACG8NZ_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0540565.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          247 a          101 c          125 g          246 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DAZKPO010000209          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000209 DAZKPO010000000
VERSION     DAZKPO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.220"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACG8NZ_002997"
     CDS             <1..600
                     /locus_tag="ACG8NZ_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0540566.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAZKPO010000210          705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000210 DAZKPO010000000
VERSION     DAZKPO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.221"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8NZ_002998"
     CDS             complement(<1..461)
                     /locus_tag="ACG8NZ_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0540567.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          224 a          128 c          101 g          252 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcga
//
LOCUS       DAZKPO010000211          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000211 DAZKPO010000000
VERSION     DAZKPO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.222"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8NZ_002999"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8NZ_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0540568.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKPO010000212          582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000212 DAZKPO010000000
VERSION     DAZKPO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.224"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>582)
                     /locus_tag="ACG8NZ_003000"
     CDS             complement(37..>582)
                     /locus_tag="ACG8NZ_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0540569.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          166 a          110 c          100 g          206 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg at
//
LOCUS       DAZKPO010000213          539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000213 DAZKPO010000000
VERSION     DAZKPO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.225"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..359)
                     /locus_tag="ACG8NZ_003001"
     CDS             complement(57..359)
                     /locus_tag="ACG8NZ_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0540570.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          185 a           92 c           75 g          187 t
ORIGIN      
        1 ttttataagt atggtgataa aatccgttcc gggctacctt ttttaggtag cctactttaa
       61 tctttatacc tttctggatc aacgaaagta tacttcaaat agtcataacg ccgatgatcg
      121 ctccgtgcgt ctggcacatc agtcacgata tcaaacaaaa aatacacatc tttcttcatt
      181 ctggtttttg ttgccggaat tttaaagtag ttcttattag agtaatagag attgattaat
      241 aagctatctt cgattgctaa aaagaaaact tctgaatccc acaccttata aaaatctttg
      301 acaaatctat tcgaaggatc aaatttaaac cataattgtg tttttccttc catcaacata
      361 atgttcacct caaaaagagt atacgaacta atgttctttt tgtaaagtag gaatatcatt
      421 gataatatgg atggaacata tatattaaaa agtgttcttt ttgaatgcta ttttgaatgc
      481 cattttcatt catttaacga aaatctgcga aaataaaaaa tgctcaaacc cttataaaa
//
LOCUS       DAZKPO010000214          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000214 DAZKPO010000000
VERSION     DAZKPO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.226"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG8NZ_003002"
     CDS             <1..354
                     /locus_tag="ACG8NZ_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0540571.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKPO010000215          519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000215 DAZKPO010000000
VERSION     DAZKPO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.227"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..189
                     /locus_tag="ACG8NZ_003003"
     CDS             67..189
                     /locus_tag="ACG8NZ_003003"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540572.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            162..293
                     /locus_tag="ACG8NZ_003004"
     CDS             162..293
                     /locus_tag="ACG8NZ_003004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540573.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          195 a           78 c           91 g          155 t
ORIGIN      
        1 aaaattaact agcaccacgg gttaaatcta tattttgata tacaaattac agcttcagat
       61 actaagttgc ataaaccttt tccggacccc atacaaaagg caattgatga attgggcata
      121 aaaaataaga aacgatttat attggtgatt cattatatga catgcaaagt gcgaaagctt
      181 gtgggatagc atttggttta gctaaatggg gagccaaaaa tatagaaaat tttcaaagtg
      241 tagatataat tattgattct cctcaagaaa tacttgattt gttagaaaaa tagacaaaga
      301 gcgtgagata aggatagggt ctcacgctct ttgtttttcg ccatttaaag attgaaattt
      361 ttgccatcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      421 aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata cccatcttac
      481 aacggatgaa cttgtaataa tagagtctta tttcaaaat
//
LOCUS       DAZKPO010000216          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000216 DAZKPO010000000
VERSION     DAZKPO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.228"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8NZ_003005"
     CDS             complement(<1..132)
                     /locus_tag="ACG8NZ_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0540574.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKPO010000217          511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000217 DAZKPO010000000
VERSION     DAZKPO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.229"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACG8NZ_003006"
     CDS             <1..342
                     /locus_tag="ACG8NZ_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540575.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           68 c           93 g          144 t
ORIGIN      
        1 gataacatac gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta
       61 tcagaagata atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta
      121 gagcgagatc ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga
      181 aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta
      241 aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct
      301 agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta
      361 atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat
      421 gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt
      481 atgtaaatgc ttataaattc ggccctataa t
//
LOCUS       DAZKPO010000218          510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000218 DAZKPO010000000
VERSION     DAZKPO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.230"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8NZ_003007"
     CDS             complement(<1..434)
                     /locus_tag="ACG8NZ_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540576.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DAZKPO010000219          487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000219 DAZKPO010000000
VERSION     DAZKPO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.232"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACG8NZ_003008"
     CDS             143..400
                     /locus_tag="ACG8NZ_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540577.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          211 a           64 c           68 g          144 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa attagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgacaaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaa
//
LOCUS       DAZKPO010000220          475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000220 DAZKPO010000000
VERSION     DAZKPO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.233"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>475
                     /locus_tag="ACG8NZ_003009"
     CDS             <1..>475
                     /locus_tag="ACG8NZ_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0540578.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           70 c          112 g          101 t
ORIGIN      
        1 ttactgcagg agaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg
       61 gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg
      121 cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa
      181 gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag
      241 tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt
      301 taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata
      361 aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg
      421 ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAZKPO010000221          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000221 DAZKPO010000000
VERSION     DAZKPO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.234"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG8NZ_003010"
     CDS             <1..>468
                     /locus_tag="ACG8NZ_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0540579.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKPO010000222          461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000222 DAZKPO010000000
VERSION     DAZKPO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.235"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>461
                     /locus_tag="ACG8NZ_003011"
     CDS             82..>461
                     /locus_tag="ACG8NZ_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0540580.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTE"
BASE COUNT          161 a           79 c           90 g          131 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa a
//
LOCUS       DAZKPO010000223          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000223 DAZKPO010000000
VERSION     DAZKPO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.236"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACG8NZ_003012"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACG8NZ_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DAZKPO010000224          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000224 DAZKPO010000000
VERSION     DAZKPO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.237"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8NZ_003013"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8NZ_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKPO010000225          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000225 DAZKPO010000000
VERSION     DAZKPO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.238"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8NZ_003014"
     rRNA            <1..>411
                     /locus_tag="ACG8NZ_003014"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKPO010000226          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000226 DAZKPO010000000
VERSION     DAZKPO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.239"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8NZ_003015"
     CDS             <1..>386
                     /locus_tag="ACG8NZ_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0540581.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKPO010000227          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000227 DAZKPO010000000
VERSION     DAZKPO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.240"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8NZ_003016"
     CDS             <1..>386
                     /locus_tag="ACG8NZ_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0540582.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKPO010000228          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000228 DAZKPO010000000
VERSION     DAZKPO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.241"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>369
                     /locus_tag="ACG8NZ_003017"
     CDS             98..>369
                     /locus_tag="ACG8NZ_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540583.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DAZKPO010000229          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000229 DAZKPO010000000
VERSION     DAZKPO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.242"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8NZ_003018"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8NZ_003018"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKPO010000230          351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000230 DAZKPO010000000
VERSION     DAZKPO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.243"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..351
                     /locus_tag="ACG8NZ_003019"
                     /pseudo
     CDS             165..351
                     /locus_tag="ACG8NZ_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          131 a           45 c           65 g          110 t
ORIGIN      
        1 aatcttcttt atagagaatt gggctatttt taatggcaaa gaggggaaga agatgtttca
       61 aaacaaagga caacgctatg ttgtaaaagg agtaaaggaa tcttactgga atcaaaaatc
      121 tttattgaga aaattatctt tgaatttaag cacaaagaaa tgaaggtaca ctagatagca
      181 atgaagatat ctttgtcatt aaacgtcgct attttacaca agtaagtgat gatttgattc
      241 atgtcttttc caatgagtgg tttgtttctg agaaaaaatt acatgcttct aatctgcaat
      301 atatgccagg tgaagatcca attcctaata tgaaagcaat tatcaacagt a
//
LOCUS       DAZKPO010000231          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000231 DAZKPO010000000
VERSION     DAZKPO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.244"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8NZ_003020"
     CDS             <1..>344
                     /locus_tag="ACG8NZ_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0540584.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKPO010000232          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000232 DAZKPO010000000
VERSION     DAZKPO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.245"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8NZ_003021"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8NZ_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0540585.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKPO010000233          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000233 DAZKPO010000000
VERSION     DAZKPO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.246"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8NZ_003022"
     CDS             <1..>321
                     /locus_tag="ACG8NZ_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0540586.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKPO010000234          302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000234 DAZKPO010000000
VERSION     DAZKPO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.249"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          107 a           33 c           48 g          114 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtttgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga tctgtggggt aaatcattat
      181 acaaaagtaa aattttactt attaacaggc tgtagaaata taaaaaagaa ctaaaattta
      241 actaaattat gtgctattct taaggaaagc caagtgtatg agaaagtttc tgagaatata
      301 at
//
LOCUS       DAZKPO010000235          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000235 DAZKPO010000000
VERSION     DAZKPO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.250"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..301
                     /locus_tag="ACG8NZ_003023"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACG8NZ_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAZKPO010000236          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000236 DAZKPO010000000
VERSION     DAZKPO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.251"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8NZ_003024"
     CDS             <1..>291
                     /locus_tag="ACG8NZ_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0540587.1"
                     /translation="GIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVP
                     KNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          100 a           62 c           52 g           77 t
ORIGIN      
        1 cggcatccac cataaaagtg ccgtcatcac actcgttgag cgactttcaa aagccattat
       61 cgccttgaaa ccagaaggcc gtaaggcagt tgatattgaa aattcgatta atgaatggct
      121 tcaatccgta cccaaaaatc ttttcaaatc aattaccttt gattgtggaa aagaattctc
      181 taattggaaa agtattagta atacgaatga tattgatatt tatttcgcag acccaggaac
      241 gccttcccaa cggggattaa atgaacattc aaacggactc ctccgaaaag a
//
LOCUS       DAZKPO010000237          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000237 DAZKPO010000000
VERSION     DAZKPO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.252"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8NZ_003025"
     CDS             <1..>291
                     /locus_tag="ACG8NZ_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0540588.1"
                     /translation="QQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQE
                     YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLR"
BASE COUNT           98 a           43 c           58 g           92 t
ORIGIN      
        1 tcagcaggat gtgattattg tagccgtggg ctactatctt cgttataacc ttagctatcg
       61 tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt tctcatacga cgatttatcg
      121 ttgggtgcaa gaatatggca aactactcta tcaaatttgg aaaaagaaaa ataaaaaatc
      181 cttttattca tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt
      241 gtatcgagcc atcgatgcag atggtttaac cttggatatt tggttacgta a
//
LOCUS       DAZKPO010000238          288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000238 DAZKPO010000000
VERSION     DAZKPO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.denovo.253"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>288
                     /locus_tag="ACG8NZ_003026"
     CDS             <1..>288
                     /locus_tag="ACG8NZ_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0540589.1"
                     /translation="AYFHQETPVAIVAKQLKRGRQTIYNVYNFLKCGGTALEYFEQYK
                     ENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCSVSTLYRR"
BASE COUNT           94 a           60 c           60 g           74 t
ORIGIN      
        1 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
       61 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      121 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt tcctgctgag
      181 gaaaaagaat acattgaaaa aagatcaact gaaggttgga ccccagacgt cattattggc
      241 cgtgcagagc gaaccttctc ttgttcagta agtaccctct atcgccgg
//
LOCUS       DAZKPO010000239        16866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.063, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000239 DAZKPO010000000
VERSION     DAZKPO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.063"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8NZ_003027"
     CDS             complement(134..577)
                     /locus_tag="ACG8NZ_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0540590.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8NZ_003028"
     CDS             complement(795..2135)
                     /locus_tag="ACG8NZ_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0540591.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8NZ_003029"
     CDS             2404..3300
                     /locus_tag="ACG8NZ_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0540592.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8NZ_003030"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8NZ_003030"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0540593.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG8NZ_003031"
     CDS             4517..5638
                     /locus_tag="ACG8NZ_003031"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0540594.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8NZ_003032"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8NZ_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0540595.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG8NZ_003033"
     CDS             7465..7992
                     /locus_tag="ACG8NZ_003033"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0540596.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG8NZ_003034"
     CDS             8203..9264
                     /locus_tag="ACG8NZ_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0540597.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG8NZ_003035"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG8NZ_003035"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0540598.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG8NZ_003036"
     CDS             10177..12159
                     /locus_tag="ACG8NZ_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0540599.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG8NZ_003037"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG8NZ_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0540600.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACG8NZ_003038"
     CDS             12881..14305
                     /locus_tag="ACG8NZ_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0540601.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ACG8NZ_003039"
     CDS             complement(14486..15259)
                     /locus_tag="ACG8NZ_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0540602.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ACG8NZ_003040"
     CDS             complement(15256..16161)
                     /locus_tag="ACG8NZ_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0540603.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="ACG8NZ_003041"
     CDS             complement(16315..16635)
                     /locus_tag="ACG8NZ_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0540604.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5434 a         3085 c         3470 g         4877 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caattt
//
LOCUS       DAZKPO010000240        15209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000240 DAZKPO010000000
VERSION     DAZKPO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.071"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACG8NZ_003042"
     CDS             complement(<1..270)
                     /locus_tag="ACG8NZ_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540605.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIA"
     gene            complement(533..3349)
                     /gene="uvrA"
                     /locus_tag="ACG8NZ_003043"
     CDS             complement(533..3349)
                     /gene="uvrA"
                     /locus_tag="ACG8NZ_003043"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0540606.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(3359..5353)
                     /gene="uvrB"
                     /locus_tag="ACG8NZ_003044"
     CDS             complement(3359..5353)
                     /gene="uvrB"
                     /locus_tag="ACG8NZ_003044"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0540607.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5612..7114
                     /locus_tag="ACG8NZ_003045"
     CDS             5612..7114
                     /locus_tag="ACG8NZ_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0540608.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            7116..7853
                     /locus_tag="ACG8NZ_003046"
     CDS             7116..7853
                     /locus_tag="ACG8NZ_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0540609.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8024..9217)
                     /locus_tag="ACG8NZ_003047"
     CDS             complement(8024..9217)
                     /locus_tag="ACG8NZ_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0540610.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(9296..9502)
                     /locus_tag="ACG8NZ_003048"
     CDS             complement(9296..9502)
                     /locus_tag="ACG8NZ_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0540611.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(9495..10637)
                     /locus_tag="ACG8NZ_003049"
     CDS             complement(9495..10637)
                     /locus_tag="ACG8NZ_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0540612.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            11149..11784
                     /locus_tag="ACG8NZ_003050"
     CDS             11149..11784
                     /locus_tag="ACG8NZ_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0540613.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            11938..12015
                     /locus_tag="ACG8NZ_003051"
                     /pseudo
     CDS             11938..12015
                     /locus_tag="ACG8NZ_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            12329..12376
                     /locus_tag="ACG8NZ_003052"
                     /pseudo
     CDS             12329..12376
                     /locus_tag="ACG8NZ_003052"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12392..14311
                     /gene="tet(M)"
                     /locus_tag="ACG8NZ_003053"
     CDS             12392..14311
                     /gene="tet(M)"
                     /locus_tag="ACG8NZ_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0540614.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14430..14597
                     /locus_tag="ACG8NZ_003054"
     CDS             14430..14597
                     /locus_tag="ACG8NZ_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0540615.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14657..15010)
                     /locus_tag="ACG8NZ_003055"
     CDS             complement(14657..15010)
                     /locus_tag="ACG8NZ_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540616.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         4476 a         3012 c         2844 g         4877 t
ORIGIN      
        1 agcaatcaaa agatattttt tactcttatc agaatatctt tcttttgcca aacgaagcat
       61 cgtctcgata aatacagggt ctttatcaaa tcctggaata acttctacat tgacaatttt
      121 actgtagcgg gtaggggcta actgatccga agcatatgct tggcagattt tgccgtatgc
      181 ttcattaaaa gaacgtgcca taccataaac gttcatgtcg cttcctaata atatagggat
      241 aaattcttca ttttcaatac tgttcatcat tatctctcac ttactcataa tctcttgttt
      301 ttcttttcca actacacaag gaatatcata ccataaagaa aaaagcaatc aaaggaaagt
      361 cctccgattg ctcaattctt ttttgaacgg attgaactta taggaagttt ttatctatac
      421 caatcagtgt agaaagaact accatgtaat gattctagaa cactgatatt aataaaacaa
      481 gcaaaaattc atttttttat gtttttttaa ctatctcagc tatctatcct ctttatacca
      541 ttattttttt caaatagtga cctgtataac tttcttttac gttaatgatt tcttctggtg
      601 ttccagtcgc aaggatcgtt cctccacctt ctccaccttc tggtcctaaa tcaatgatat
      661 aatctgctgt tttgattaca tctagattgt gttcaatcac taaaactgtg ttaccagcat
      721 ctactaatct ttgtaataca tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg
      781 ttggttcatc tagtatatag aaattctttc cattagagat tttgtgcaat tcactggcaa
      841 gtttcatccg ttgtgcctca ccaccggaca atgtcgttgc tggttgcccc atcgtcacat
      901 aacctaagcc aacatcaaca atcgtttgca gtttgcgatg aatctttgga atgtgcttga
      961 agaattctac agcatcctct actgtcattt ccaaaatatc agcaatgctt tttcctttgt
     1021 aatgcacttc taatgtttca gaattatatc gtttaccatg acatacttca caaggaacat
     1081 agacatcagg caaaaagtgc atttcgatct taataattcc atccccgcga caagcttcac
     1141 aacgaccgcc ttttacgttg aaactaaaac gccctttctt ataaccccgc atttttgcct
     1201 cgttcgtttg agcaaataac ccacggatat catcaaatac acttgtataa gtcgctggat
     1261 tacttctcgg cgtccggccg attgggcttt gatcgatatc gatgatcttt tcgatacttt
     1321 cgtagccgga aattgtcttg aatttacctg gcttagcaga attcttattt aatttttgcg
     1381 ctaacgattt ctttaagata ctattgacca atgtactctt tccagaacca gacacacctg
     1441 tgactgctac aaattctcct aatggaaatt caacactaat atttttcaga ttgttttctg
     1501 aagcaccagt gatcttgatt gctttcccat tacctttacg gcgttctttt ggtacaggga
     1561 ttgatttttt ccctgaaaga tatttccctg tcaaagaatt ttcatcatta gctacttctt
     1621 caggagtgcc tgcagccacg atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa
     1681 tcaaataatc tgctgcccac atcgtatctt catcatgttc gactacaacg agggtgttgc
     1741 caagatcacg cattttcttt aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc
     1801 cgatcgatgg ttcatctaaa atatacaaaa ctccagaaag attcgatcca atttgtgtag
     1861 ccaatctgat tcgctgcgct tctcccccag ataatgttcc ggaagcgcga ctcaaagtta
     1921 gatagtctag tcccacattt tccaagaatg aaagtctgtc atttacttct tttaaaatcg
     1981 gtttggcaat tactttctct tgttcagata acgttaagtt tgagaagaaa tctactgctt
     2041 tttgaataga caactcattt gtttgcccga tattcgttcc attgatttga acagatagag
     2101 cttggggatt caatcgataa cctttacatg tctgacaagt taattctgtc atataaagac
     2161 gcatttgctc acgcgtgaaa tcactgttcg tatcatgata acgacgctcg atattcttca
     2221 ataccccttc gaaagtcgtt tctacatcgc gaacgccacc aaaatcattt tcataatgga
     2281 aatggaaaag atcatctgtc ccattcaaga tgatctcctg atgctcttcc ggtaattctt
     2341 cgaatggtgt atctaaatcg ataccgaaac tttcacaagc ctgcgctaac atttgaggat
     2401 aatactgaga gctgattgga ttccatggaa cgatagcacc ttgactaagt gtcttcgttc
     2461 tatctggaat cactaaatct tgatcgactt ctaatttgac acctaaacca tcacagtctg
     2521 gacaagctcc aaaaggcgca ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa
     2581 atccgcaata aggacaggca tagtgttcgc taaacaacat ttcttcttca ccgatcacgt
     2641 caaccagagc atagccgtct gctaatcgca gggccgcctc taaagaatca aataggcgag
     2701 aacgaatccc ttctttcaca atgatccggt cgatgacgat cgcgatatca tgttttttgt
     2761 ttttctccag ctcaggcact tcacttaaat catagatctc accatcgact cgaacgcgga
     2821 cgtatccctc tttttggatt cgttcgaata tttttttatg ttgtcctttt ttctttacca
     2881 cgatcggtgc caacacttgg atctttgtgc gctcaggcaa agccattact tgatctgcca
     2941 tttgatctac tgattgactg gtaatctcga tatgatcgtt gggacaaatc ggatgtccta
     3001 cacgtgcaaa caacaagcgt aaatagtcat tgatttccgt cacagtacct actgtcgacc
     3061 gaggattttt acttgttgtt ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc
     3121 tatctacgtc cggcttatcc atttgtccta aaaactgacg agcataagca gaaaggctct
     3181 ctacgtatct ccgctgccct tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag
     3241 aaccggacaa acctgtaacg accacgaatt tatctcgtgg aatcgttaca tctatatttt
     3301 tcaagttatg ggcacgtgca ccatgaatcg taattttatc gttagccatg ttgtcctcct
     3361 actttgaagc ttttaattct aaaatcatat ctcgcacatt cgcggctgtt tcaaaatcca
     3421 aagcttttgc cgcatctttc atttcttttt ctaatttcat caataacgtg tctttttctt
     3481 ccttcgtcat ttcttcgtac gagacttgta ccgcttcttt tgtatcatct tccgattctt
     3541 tcgtaataga aatcaaatct cggatttctt taatgatcgt ttttggaacg atcccatgtt
     3601 cttcattgta cttctgctga atcgtacgac gtcttgacgt ttcatccatc gctagacgca
     3661 tagaatctgt tactttgtct gcgtacatga ttactttacc ttcttcattc cgagcagctc
     3721 gtccaatcgt ttggactaag gaacgttcac tacgtaaaaa gccttcttta tccgcatcca
     3781 agatagcaac aagggaaact tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa
     3841 ctaacacatc aaattcacct aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt
     3901 tgatatcact atgcaaatat ttgactttga tccctaattc tttgaaataa tctgtgaggt
     3961 cttctgccat ttttttcgtc aatgtcgtta caaacacccg ctggtctttt tcgactcgtt
     4021 catttatttc gcctactaga tcatcgattt gtcccatgat tggacgaact tcgatcactg
     4081 ggtcaagcag ccctgtcgga cggatgattt gttggataac ggtatctgtt tgttcatgtt
     4141 catatggtcc tggcgtcgca gagacataaa tgatctgatt gacatgtttt tcaaactctt
     4201 ctaaccgaag tggccgattg tctaaagcag atggcaaacg gaaaccgtaa tctactagca
     4261 tctgttttct cgctcggtca ccattataca ttcctctgat ttgaggcatg gttacgtgcg
     4321 attcgtccgc cacgatcaaa aaatcttcag gaaagaaatc caatagcgta taaggcggtt
     4381 caccttcttt acgtccgtcc atatgccgag aataattttc aattcccgac gtgtagccca
     4441 tttctaacat catttcaata tcgtaattgg ttcgctgttc taaacgttga gcttccagaa
     4501 gtttgttttc atttcgcaag acagttaaac gctgttctaa ttcctctttg atattcgcta
     4561 cggcatgctc catatgttca tcattcgtca caaagtgagt cgccgggaag attgctacgt
     4621 gttccgtttc tcctaaaact tcacctgtca gtgcattgac ttctcggata cgttcgacct
     4681 catctccaaa aaactccaca cgtaaggcac gttcatctct tgaagccgga aaaatctcca
     4741 cgacatcacc gcgcactcga aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt
     4801 ggatactgac taagtcacga atcaactgat tgcgatctag ttctgctccc tgtcgaatcg
     4861 aaactacttg tttttggtac tcaaaaggcg agcctaatcc aaaaatacat gaaacggatg
     4921 caatgacgat cacatcattt ctttctaata atgaactagt agccgaatgt cgtaatttgt
     4981 cgatctcatc atttacactt gaatcttttt cgatatacgt atcgcttgaa ggcacataag
     5041 cttcaggctg ataataatca taataagaaa caaaatattc aacagcattg ttagggaaaa
     5101 attccttgaa ttccccgtac agctgaccag ccaatgtttt gttatgggca ataatcagtg
     5161 ttggtttatt cactttctca ataagattgg aaatcgtata tgttttccct gttccagtcg
     5221 cacctaataa gatttgtgct tttttcccgc cgactacgcc atccactaat tggttaatcg
     5281 cttcaggctg atcaccagcc ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat
     5341 ctctctcaat cattcttcgt cctccttttc attcattcct tttcatttta cgaccaataa
     5401 aaagaaaagg aaagcaaagg cattccttac taaaaaatta taccacaccg aacatacttt
     5461 cgccactttt ttatcttatt atacatgtta tgttttataa cataaaatgt tagaaactcg
     5521 catttttttc attttttcga gcttacagtc ttgtctgatt cagcactttt cgcctataat
     5581 aataaaatat tggaaattta ggaggttgat tatgaaaaga aagcatttat ttcactcatt
     5641 tgttttctta atgacaggac ttgtgacact tgcgtttggt catgttgcac aagctgacaa
     5701 cactacaacg gcagagtcaa gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga
     5761 cgtatacgta attgccagtg actctgcttt tgctccattt gaatttcaaa acgcaaaggg
     5821 cgagtacgaa ggcatcgatg taaaattgat ggaaaacatc gccaaactgc aaggatttaa
     5881 catccgaatg gactaccgtg gtttctctgc tgctttgcaa gctttggaat caggacaagc
     5941 agacggaatg attgccggaa tgactgtaac agatgaacga aagaaaagct atgatttttc
     6001 tgattcttac tttgatagcg ggatccaatt agctgtcaaa aaaggagata cttcgatcaa
     6061 gtcttatgaa gatttgaaag gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga
     6121 atttttaagc gaccatgaat ctgaatatgg ctattctatc aaatattttg atgctgctga
     6181 tcaattgtac gacgggttaa aagttggcgc aatcgatgca atgatggatg attatccggt
     6241 aatcggttat gcgatcaaac aaggacaaga attggaaaca ccgatcaaaa aagaatctgg
     6301 cggaaaatat gcatttgccg ttaaaaaagg acaaaatcct gagcttctta atatgttcaa
     6361 tgaaggattg aaagaattaa aaagaacagg cgaatacgat aagattatta aagaatatgt
     6421 agcggatggt tcagaaactt ccagtgagtc ttccactgct gatgaatcaa catttcttgg
     6481 acttatccaa aacaactatc aagcattgtt gaaaggactt tggagaacaa ttctcctaac
     6541 tttgatctcc tttgctttgg ctatagtaat cggtgtgatt tttggattat tcagtgtagc
     6601 accaatacgc gctcttcgca caattgctag tatttatgtc gatatcatcc gaggaattcc
     6661 aatgatggtt cttgcgttct ttattttctt tgggttgcct ggtatcgttg gatttacgat
     6721 tcctgacttc cttgctggtg ttatcacctt gactcttaac gcaagtgctt atatcgcaga
     6781 gattgttcga ggcgggatca acgccgttcc tgtcggacaa atggaagctt ccagaagttt
     6841 aggtctttct tataatcgga caatgcaaaa aatcattttg ccgcaagcga tacgcatcat
     6901 gatcccatca ttcgtcaatc agttcgttat ctcattaaaa gacacaacga tcatttccgc
     6961 aatcggtgta gtcgaattgc tgcaaactgg taagatcatt gttgcacgta caacacaaag
     7021 tacgtatgtt tacttgatca ttgcgatcat gtacttgatt ttgatcacag cattgacaaa
     7081 attagcaaaa gttttagaaa agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc
     7141 atttagtcaa aaaatacggc gataacacag tattaaacga catcaatgta tccatcaacg
     7201 aaggagatgt cgtctgtatc attggccctt caggttcagg aaaaagtaca ttcttgcgtt
     7261 gcttgaatcg tttagaagaa ccttctgacg gtgaaattat tattgacggc gcccacttga
     7321 tggataaaag cacagatatc aacttggtga gacaacatat cggaatggta ttccaacact
     7381 ttaacctatt ccctcatctt tctattttgg aaaacatcat tttagcaccg atggacctga
     7441 agaaagaatc tagagaagaa gcagagaaaa aagcaatcaa attactgcag acagttggtc
     7501 tagaagataa aaaagatatg tatcctgaaa tgctttctgg tggacaaaaa cagcgtgtgg
     7561 ctattgctcg tgctctagcg atgaaccctg acatcatgct ttttgatgag cctacttctg
     7621 cactcgatcc tgaaatggtc ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag
     7681 gtatgacgat ggttatcgtt acgcacgaaa tggggttcgc taaagaagtg gccaatcgcg
     7741 tcatgttcat tgacgacgga aactttttag aagatggaac gcctcaacaa atctttgaaa
     7801 atccgcaaaa tgaacggaca aaagatttct tgaataaagt attaaatatt taaaataaat
     7861 tatatatgtc tttttgacta tctttttata tcttcttaaa tgtccgcaaa gccttatttt
     7921 ataggctcta cgggcatttt gcttttgtgg taaacctcac atatctaggt ctatcttctt
     7981 atattttcac tatcaagcgt ggttaaaatc gtggtaaata cttctatgca attagacgct
     8041 gaacctctga ttttgcggta tctatggacg catgagcata ccagttcatt gtgatactga
     8101 tatttgaatg ccccatgata tactgtaaat cttttgggtt catgttcttg cttgccagtc
     8161 ttgtgcagaa tgtatggcgt agcgtatgcg gtgtgatatg tggcaatgga ttgtccttgt
     8221 ggtgcttgtt gtatttcttt accatacgga taaataaggc gttataatca atcgcaactt
     8281 tgggcttgcc cttatgattg acaaatagga agttgccccg tccgtctatc acaaatggtt
     8341 ctgcctttgg acgttttttc ataacctgtt gaaatgcctg tatcgtttct ttgcttaatg
     8401 gtacttgcct tattccgctt tttgttttag gcgtttcaat ataatagccc tgttccttgc
     8461 tctttagtaa ctggtggtcg ataatcacaa cttcattctt gaaatcaatg tcggctactg
     8521 tcagtccgca cagttccgaa atacgaagtc ctgtctttaa cagtatcagc acatcatcat
     8581 aatgcttgtg atacacattg tccgtcttga tgaatgagag taaggcttgt tcctgttcct
     8641 ctgtcaatgc gactttcttt ttggtatcat tttctaggac ttcactcaac ttgaaatcaa
     8701 agggattttt cctcacataa tcatcttgaa tggctatgta gaatgacgct tttaacgagc
     8761 gtttatggtt gttaatggta ttgtaggaaa agcctttgtc tttcatgcgt aacgcccatt
     8821 ccttagcgtc agagggtttg attgtatcaa tgctcctagc acctaacttg tcctctttca
     8881 ataaccgcat gagttgttcc cgttgtttct gtgtgctttt cttcacattt gccctctgtg
     8941 cgttctgttt ggcataaagt tggcaaagcg tcattttctt gccagtgctg tcgataccgt
     9001 cctcaatatc ccgtcttatc tgctgttcca gttctcgaag tgagggtttt tcccgttttc
     9061 cctttggtgt cgggtctgtg ggtgtcaatc tccaagcata cacatatttt gtgtttccaa
     9121 atgcgtctac atatttatat aagtattttc tgtctgttcg ttggctctct ccagtatgca
     9181 ggatacgacc tttgttatcc cgtctttttt ctttcatggt gtctgctcct ttccttgttg
     9241 gaaagagcct tgatatgact tgtgttcatc ataacacata caaggctcat ttgcattaga
     9301 tagcgtccaa tttatcaatg acctgttcaa actgtcggcg tttaatctgt atgcggttgc
     9361 cattcataat gagccagcca gcgtccttgt tttcctctgc caatcgtctt aacttgtttt
     9421 ctccgatacg gaaatacttt gacgcttctt caatggtaag ggtgtatttt tcccatacgg
     9481 gaatatcctg tgcattactc ataaaacacc ccctcacttt cgattacgtc tgctatgtcc
     9541 gtacacatct gttccacaag catttcctgc ttttcagaga ggtgggtaca ttcatcaatc
     9601 attttcatgt agtttgagcc tgtcagcctg tcgatttcct ctatcatcac gattgacggc
     9661 atgacctgtc tttccagcca ttgcagagtg cgttccatat tgacaggtac ggggttcatt
     9721 tcaaaaggta aactgtcatg ggaaatgaat atctcccaca gcggatagtc gggaaattgc
     9781 acaaaatcag taataaggta aaacaccagc ccatgagggt tatatgtcaa taacaattcc
     9841 tcaactgcct gtactgcttt tgtaccacgc agacgaattt caaaacgatt gataatatcc
     9901 gtatgtttct ttttggttgc ctgttccttt tgtttttcat acaggcagaa gtattttgtg
     9961 cttgcctttg aaccgatata aagggtgctt gctaaattac ggttttttcc gcttagtttt
    10021 ccaccctgca cattttcata atttttacag cgacaatccg caccgccatt tctgtatttt
    10081 tcagacaaaa cgggaatatc caacagacca cacatatcat tgattgccaa gtcaaaacgt
    10141 cggataacac cgtcacagtc aagacagcgg tttaaaaatg aataccaatc tctgttctgt
    10201 gcctgcaaga catattccat gttgcgtgag cctgcccctt ttaattccaa gaatacgccc
    10261 atgtgttcgt catcagaagc catgaccttg atttctccgt aggcgtaatg ctctttatag
    10321 ccataatacc catagtcata atggataaaa taatctgctt tcatgccaag cacctttcgg
    10381 ataatttcca atgcgtcagt cgttggataa cggacagaca gataatcaac gagcagataa
    10441 aaaggcttat cgcagatata ataattcaga caacgcagga taatattttg tgtttccttg
    10501 ctggcgttgg cttttccgtt ctcaaagcgg ttgatactat atcttgatac atgggtaaga
    10561 cttgcaagtt ctccttgtga aagaccgctt gcaagacgtt tgttcttcat ttcaagtgca
    10621 aattctaaat tgttcattgt attcctccaa tcccacaata gccttgaaat acctgtaaac
    10681 ctctattcaa aaagtgtgat atgcactatc ttcttaaaac ttctcattac tactcacaac
    10741 tttgggattg tatggttttt gacttgtctt ttgtgaaata gtgcccccct gttagataac
    10801 gggggcacag acaaggctcg caggctcgcc agccgacaaa gcatttcttc cccttgacac
    10861 aaggtgtcat ggcggtaagc ctgagaggct tccgccaccg tgtcagaaat actttgtcag
    10921 caagttctgt tttgcctttt ctaacaagtt ccatttcaac cgatattcat atctacggat
    10981 ttaatcagat aggtatttct ttttgaatgt ttttatattt cattctattg tgcttattcg
    11041 attacaagaa catagtaatt cttatatgct tatttgtcaa tatctttttt tgcctttaca
    11101 aatcatatta agcatgatag acttataata gcgaagatat aacgagatat ggagggttat
    11161 gcaatgaaaa taagccattt aggaaataat atacagacca taagaaaatt taggggaatg
    11221 aaacaacagg aacttgcgga caaaatcggt atcaatatgc agagcctttc caagattgaa
    11281 agagggttga actatcctgc ctatgaaacg ctggaaaaga taatggaagt gctggacgtt
    11341 acgcccaatg aattgttgtc gggagaatgg aagtatgtca atcaatctga aaaggaagtc
    11401 tgtcagtttt taagagccga agaacgtcta aatgcagaac tgaaacatgg acactatgat
    11461 aacttctttg acagcgagga agaatggctg gaatatgagt tagaaaagtt acgggaatat
    11521 attacagact atatcaacgg gaaaagcatt gaagcgtccg acctttaccc aatcaaagaa
    11581 tttatccagc atttgaagtt tcaaaaatta ttagaccgct atgatgattt atacagtatg
    11641 gatatgtttg gggaaagcat agagggacat aagtataaga caccttatca agtcgtgaaa
    11701 atgataaacc cgaactcaaa agaagatatg gaactgttac gagaagtatt aaggaataac
    11761 aattttgatg atgaggacga gtaacttttt tcctcttgcg agaagataca gcaaacaaaa
    11821 agcccgatca agagtgcaaa gcaccaccaa tggtggcaaa gctcttgaca gggctttttc
    11881 ttttctgttg tgggcaatca agaggaagaa agggataaaa tctgatttgt ggtaaatcag
    11941 gcaaaggcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg
    12001 aagattatag aataacaaat attggtacat tattacagct attttgtaat cacgtactct
    12061 ctttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta
    12121 tctatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
    12181 atagtgggaa caaataattg gatgtccttt tttaggaggg cttagttttt tgtacccagt
    12241 ttaagaatac ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat
    12301 gcctatggtt atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc
    12361 ccttttgggt ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
    12421 tcatgttgat gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
    12481 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
    12541 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
    12601 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
    12661 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
    12721 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
    12781 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
    12841 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
    12901 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
    12961 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
    13021 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
    13081 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
    13141 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct
    13201 ttatagtgga gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa
    13261 agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc
    13321 tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac
    13381 aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt
    13441 tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga
    13501 tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt
    13561 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
    13621 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
    13681 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
    13741 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
    13801 tcaatcgttt caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta
    13861 tggttggaat gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt
    13921 tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa
    13981 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
    14041 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
    14101 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
    14161 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
    14221 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
    14281 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
    14341 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
    14401 agatagttat tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat
    14461 taaagataag ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac
    14521 aagaaaattt aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa
    14581 agacgcagag ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat
    14641 gtatggattc ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag
    14701 attccattaa ggatagttct ttgtctgtaa agctatccat gtatttctct atctgtaatc
    14761 gtcgggtgct ttttaccaag ttattagcag gtaagaaaaa ttcatcaacg gaaacatgaa
    14821 gtaacgatac aaggtcataa agaacttgta tgctggggtg ttgcccttta ttttcaatat
    14881 tagttaagta ccgtgggtca atttcaatca atgctcccac ttgttcacga gttaaacctc
    14941 gtttcaatcg agcttcttta atggctaaac caaaggctct aaaatcatat ttatcttctt
    15001 ttttacgcat agtagaccac ctctatacat tttattgttc ctactgaatt aaaaacaggt
    15061 atagaaaaac gtgttatatg gtttataggt ttatatttaa taaaaagcac tactaaacgc
    15121 caataaaaaa aaccgttata tggtagtgct atttacgctg ttaaaatatt gtatattact
    15181 tccaaatggc ggtttgttgg aggtcaacg
//
LOCUS       DAZKPO010000241         8315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000241 DAZKPO010000000
VERSION     DAZKPO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.108"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..726
                     /locus_tag="ACG8NZ_003056"
     CDS             79..726
                     /locus_tag="ACG8NZ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0540617.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1419..2357)
                     /locus_tag="ACG8NZ_003057"
                     /pseudo
     CDS             complement(1419..2357)
                     /locus_tag="ACG8NZ_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2357..2974)
                     /locus_tag="ACG8NZ_003058"
     CDS             complement(2357..2974)
                     /locus_tag="ACG8NZ_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0540618.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2988..3158)
                     /locus_tag="ACG8NZ_003059"
     CDS             complement(2988..3158)
                     /locus_tag="ACG8NZ_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540619.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3506..4996)
                     /locus_tag="ACG8NZ_003060"
     CDS             complement(3506..4996)
                     /locus_tag="ACG8NZ_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0540620.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5039..5338)
                     /locus_tag="ACG8NZ_003061"
     CDS             complement(5039..5338)
                     /locus_tag="ACG8NZ_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540621.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5704..5988)
                     /locus_tag="ACG8NZ_003062"
     CDS             complement(5704..5988)
                     /locus_tag="ACG8NZ_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0540622.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6507..6992)
                     /gene="vanZ-A"
                     /locus_tag="ACG8NZ_003063"
     CDS             complement(6507..6992)
                     /gene="vanZ-A"
                     /locus_tag="ACG8NZ_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0540623.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(7145..8056)
                     /locus_tag="ACG8NZ_003064"
     CDS             complement(7145..8056)
                     /locus_tag="ACG8NZ_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0540624.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2541 a         1478 c         1244 g         3052 t
ORIGIN      
        1 aggtgacata acgtatgaaa aaatcagagg attattcctc ctaaatataa aaatttaaaa
       61 tttaggagga agttatatat gacttttaat attattgaat tagaaaattg ggatagaaaa
      121 gaatattttg aacactattt taatcagcaa actacttata gcattactaa agaaattgat
      181 attactttgt ttaaagatat gataaaaaag aaaggatatg aaatttatcc ctctttaatt
      241 tatgcaatta tggaagttgt aaataaaaat aaagtgttta gaacaggaat taatagtgag
      301 aataaattag gttattggga taagttaaat cctttgtata cagtttttaa taagcaaact
      361 gaaaaattta ctaacatttg gactgaatct gataaaaact tcatttcttt ttataataat
      421 tataaaaatg acttgcttga atataaagat aaagaagaaa tgtttcctaa aaaaccgata
      481 cctgaaaaca ccataccgat ttcaatgatt ccttggattg attttagttc atttaattta
      541 aatattggta acaatagcag ctttttattg cctattatta cgataggtaa attttatagt
      601 gagaataata aaatttatat accagttgct ctgcaacttc atcattctgt atgtgatggt
      661 taccatgctt cactatttat gaatgaattt caagatataa ttcatagggt agatgattgg
      721 atttagtttt tagattttga aagtgaattt aattttatac acgtaagtga tcataaaatt
      781 tatgaacgta taacaaccac attttttggt tgcttgtggt tttgattttg aatttggttt
      841 tgaacttatg gactgattta ttcagtccat tttttgtgct tgcacaaaaa ctagcctcgc
      901 agagcacacg cattaatgac ttatgaaacg tagtaaataa gtctagtgtg ttatacttta
      961 cttggaagat gcaccgaata aaaaatattg aagaacaact agcaaaagat tttaaagagt
     1021 tattttattt taagtcttta taacatgagt gaagcgaatt tttaaatttc gatagaaatt
     1081 tttacatcaa aaagccccct gtcaaaattg acgaaggggg ttttttggcg cacgcttttc
     1141 gttagaaata tacaagattg aaaatcgtgt ataagtgcgc cctttgtttt gaacttagca
     1201 cgttacatca attttttaaa atgatgtata agtgcgccct tttaaatttt gagtgattat
     1261 attttttgag ttagaaaaag ggattgggaa aatttcccaa aataatttaa aaaataagca
     1321 aaaattttcg atagagaatg tgctattttt tgtcaaaggt gtataccttg actgtgcttg
     1381 ctgttacatt aagtttattt ttaagttatt aaaaaagaaa tagcttttaa agtttggctc
     1441 gctgtcgctt tataaagctg attgactttt gattgcaaac tacttaaaga aaacaaactc
     1501 ggactattcg ttttcttctc tttggtttga acatcagcaa ttatcccctc ttgattgcct
     1561 attttagctt gtttagaaga aacaaaagct aaaagctcct cttgggtttt aaaacgctgt
     1621 gtggggctta gaacgccctt aaacgaccct tggtttactt ttatactagc ttccacctcg
     1681 aaaaaaggtt cttttttaaa attctctatg gcttcctggc gctgaaaaat aaggtataag
     1741 gtgggcgttt gaacacgtcc tagtgaaaat gtaccttgta cgcccttctg ttgtaaattt
     1801 aacgtataca aagggcttgc gttcatgccg atcaaccaat cggcaatttg gcgtgtttgc
     1861 gcttcttgat aaaagggata gtaattcatt ccaggttgca aattttgaaa accgcttcgg
     1921 attacatctt tttctaagct attgatccat agtcttttaa atgttttatc ttttgaaaag
     1981 gcatttgctt tatggataat cgaccaggcg atattttcac cttctctgtc gctatctgtt
     2041 gcaacaataa ttgtatttgc ctttttgaga agttctgcaa caattttaaa ctgctttccc
     2101 ttatcttttg caacttcaaa atcgtatcga tcaggaaaaa tcggcaaaga ttcaagtttc
     2161 caattttgcc acttttcgtc ataatgacct ggttctgcta attccactaa atgcccaaaa
     2221 ccaaaggtga taaacgtttc atctgtaaat agtgggtctt tgatctcaaa ataaccgtct
     2281 tttttggtgc tttgttttaa agcacttgcg taggctaatg cctggcttgg tttttcagct
     2341 aaaataaccg tactcattaa ctatccctct tttcattgtt ttttctttga tcgactgtca
     2401 cgttatatct tgctcgatac cttctaaacg ttcggcgatt gattccagtt tgttcttcaa
     2461 cttctttatc ggataaacca ttcaaaaaca aatcgaaagc atgctttaaa cgtggatcat
     2521 tttctttaaa tttatgctga cgacctttga atttgccttt tttcttagca atttcgattc
     2581 cttgtgcctg acgttcctta attttttttc gttctgattc tgcttgatac ttgtacaatt
     2641 caatgacaag gctattaatc aaacgcctta aattttcatc ttcaatacca ttcattgagg
     2701 gtaaatttaa gacttccagg gttgccccct taatttgaat ttgattcatc aattctgtta
     2761 attctttatt atttcgtcct aatcgatcta attcagtaac aataacaata tccccttcac
     2821 gaatatagtt aagcatagct tgtaattgtg ggcgttcgac cgattgaccg cttaatttgt
     2881 ctgaaaagac cttagaaacg ccctgtaacg cttgtaattg ccgatctaag ttctgttctt
     2941 tgctactgac acgtgcataa ccaattttag ccattttcaa ccaacctcta aaattctctc
     3001 ggttgcaata accaatcagc aatatctact ttttcaattt caaattgctt atcagaaatt
     3061 gtcttttcgt aagcgataaa atcttgcgca tattgttgct cattaaaaat agccaccact
     3121 tcgtcatttt ctaaaactcg ataaataaat tttttcattt tactcctcct attatgccca
     3181 acttaaatga cctattcacc aagtcaatta tactgctaaa atcatattag gacaaatagg
     3241 tatactctaa tgacctataa aagatagaaa attaaaaaat caagtgttcg cttcgctctc
     3301 actgcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
     3361 gttttcgggc tactctctcc ttctccccct aataattaat taaaatctta ctctgtatat
     3421 ttctgctaat cattcgctaa acagcaaaga aaaaacaaac acgtatcata gatataaatg
     3481 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
     3541 tgtccgtttt agggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgctcta
     3601 agtcaaaaga atttgtcagc gcctttatat agctttcttt ttcttctttt tttactttaa
     3661 tgatcgatag caacaatgat ttaacactag caagttgaat gccaccattt cttcctggtt
     3721 taatcttaaa gaaaatttcc tgattcgcct tcagtacctt cagcaattta tctaatgtcc
     3781 gttcaggaat gcctagcact tctctaatct cttttttggt cgtcactaaa taaggcttgt
     3841 atacatcgct tttttcgcta atataagcca ttaaatcttc tttccattct gacaaatgaa
     3901 cacgttgacg ttcgcttctt tttttcttga atttaaacca cccttgacgg acaaataaat
     3961 ctttactggt taaatcactt gatacccaag ctttgcaaag aatggtaatg tattccctat
     4021 tagccccttg atagttttct gaataggcac ttctaacaat tttgattact tctttttctt
     4081 ctaagggttg atctaatcga ttattaaact caaacatatt atattcgcac gtttcgattg
     4141 aatagcctga actaaagtag gctaaagaga gggtaaacat gacgttatta cgccctatta
     4201 aacccttttc tcctgaaaat ttcgtttcgt gcaataagag attaaaccag ggttcatcta
     4261 cttgtttttt gccttctgta ccgcttaaaa ccgttagact tgaacgagta aagcccttat
     4321 tatctgtttg tttgaaagac caatcttgcc attctttgaa agaataacgg taattaggat
     4381 caaaaaattc tacattgtcc gttcttggta tgcgagcaat accaaaatga ttacacgtta
     4441 gatcaactgg caaagacttt ccaaaatatt ctcggatatt ttgcgaaatt attttggctg
     4501 ctttgacaga tttaaattct gattttgaag tcacatagac tggcgtttct aaaacaaaat
     4561 atgcttgata acctttatca gatttgataa tcatagtagg cataaaacct aaatcaatag
     4621 cggttgttaa aatatcgctt gctgaaatag tttcttttgc cgtgtgaata tcaaaatcaa
     4681 taaagaaggt attgatttgt cttaaattgt tttcagaatg tcctttcgtg tatgaacggt
     4741 tttcgtctgc atacgttcca taacgataaa cgttgggtgt ccaatgtgta aatgtatctt
     4801 gattttcttg aatcgcttcc tcggaagtca gaacaacacc acgaccgcca atcatgcttg
     4861 attttgagcg atacgcaaaa atagcccctt tgcttttacc tggcttggta gtgattgagc
     4921 gaattttact atttttaaat ttgtacttta acaagccgtc atgaagcaca gtttctacaa
     4981 caaaagggat attcattcag ctgttctcct ttcctataaa tcctataaaa taggttgttt
     5041 aattaacttg gtttgctttt tcattcaact gtttcaatat tgcatgtttt gaaaaagatt
     5101 tttttccttt ataagtcaat ttttttccac taatcgaata aattattttg ttattttcta
     5161 ttaacttata tatataatct tccccctccg aagaaaaata cttatctgat tttgtttcta
     5221 agtagatatt tctcttttct aactctttct taaacgtttc tagtgtatag atatttgcta
     5281 attttcttat ctccaataaa ctatttttta tataagtttt acattcatca tgattcatac
     5341 aaactccacc ttctataaat gaatacaaaa aaagcaatca aacgatttcc gattgattgc
     5401 ttaacaattc ttaaattcag tagcttagat acttgaaaac tctctgattt ccctatataa
     5461 tgatagtacg gttatatacc gtcttcaaac aaagttaatt aaataacttc ttacgaggga
     5521 agagttcatc tgactaactg ataagcgttg gtttggcaat cttatcgggc tatgcattta
     5581 taaaatgtcg tcaaacattt tataaatgtg tcatggctct tttttcgttt ctattcagtt
     5641 cgttgtttcg ttatatctag tataccgctt ttaaaaaaaa taagcaacga tttcgtgcat
     5701 tattcacacg aagtcattgc ttttttcttc ttccatttct aaatccaatg ttacttgttc
     5761 tgattctgtt tctggttctg gttctgttgg ctcatttggg attaaatcca ctactagcgt
     5821 tgagttagtt aactttgcaa tttgttctag tgtttttatg gttggatctg attttcctga
     5881 ttctattcgt gaataatttg atctactcat ttctaattct tgggagaatt tttcttttga
     5941 tttggtacct ctcatctttt ttaagctttc tctaaatgct agttccaagt ttctcccccc
     6001 tctttttgtg taataaaatt cacgctttat ttaaattata gcttttaata tcttaataaa
     6061 caagtgatta atcacttgtg atttgtgatt attccaaaag taggtaattt caccaacctt
     6121 tttcaaaagt ttttatcgac gattattgga ctggatcgtg tttcctaaag cgattctaag
     6181 cgcatgttta attttaaagt gggtaattct ctttaaaacg tcctacaaag attcaaaaaa
     6241 agcatggctg ctagtggggg taccgtcagg aaaatgcgga tttacaacgt taaggaaatt
     6301 tattaaagag accaattcct cagcactaaa attgataatt gggctttctt tgttactata
     6361 gtgttaggta agctttttac tttatcaccg gttttcccct cacttcacac ctacgggcga
     6421 gtttccccac atcccgtttt ccatctaatc caattcattc aatatcttta tgtgtatgaa
     6481 agatcagatt gcttgattta gacatcttac acgtaattta ttcttaaatg ggtacggtaa
     6541 acgagcaata atacgagcaa aagtatacct acaaaaataa taactctgtc taattttttt
     6601 tgattcatat gcttattgct taaaccatat aatttcagtc caagaaagcc tccaacagta
     6661 tttgtaatta catctgttat gtctgtcgct ccaatagcga agataaattg aattatttca
     6721 aaagtaagac ttaaaaccag tacaaaagca aacttaggta aaaatccgat ttctttaaaa
     6781 ttgacattca aaagcaagcc aaatggaata aagattataa cattatctat catctctctg
     6841 aaattcccag tagcagtaaa tggagtcaag ttaagacttc tttgatgata attaaatact
     6901 gataaaatat tgtattgtaa tttgaataac actaaccaga ttagtgtcac taaatataaa
     6961 gctagcaatc ctctagataa tatttttccc aatataattt cctctaattc ttttgtttaa
     7021 gttgtatcat tagtatagca atgtcctagt taacgtgggt tttaaattaa aaagctaacg
     7081 ttaccccaaa agtgctagta caataatgct gttcattaat ttcgttggtt tcattccgcc
     7141 atccttacct ccttgaatta gtatgtgttg atccgggaaa cactgtcaca attacaccgt
     7201 ctatattgtt tcctgatatc tcataacgaa gattagtcgg cacatgaatg gtggtatttt
     7261 tagtaacagg ataataaaag atctcatatt tttccccatt tacactaaca gaaatggttt
     7321 tttcttcttt taggtaatcc atatattcct cgagaacgaa attcttttct ttcataatcg
     7381 cactatgtgg taaaccaaca tagcgaatat gccatggttc atattgaatt cctgttaact
     7441 ctgttttgtc ctctggataa cgtaaaatga acccgtattt ccaagcattt tcttctatcc
     7501 actttccttc aggggctcgt tccattttcg tcaagcttga tcctacatct agtgataaac
     7561 ctgaattatg ctcactataa cctgctggta aggcatactc agcccccatt tcttggtaaa
     7621 gcacactttg ctcatcaaag tctcgatagc cactattaat aataaaatga ctaacgccac
     7681 cctttacagc atcattgacc atctctgaaa atttttgtgc tatttctttt gacatataaa
     7741 tattactatc aagcaacccg tatccattta ttaattcgtc atgtttagat aaattcacga
     7801 tatctgactt cacactttct tggcgaacag gatatttact attgattaat agcagatttc
     7861 cttgataaac ctgttcttct gtaattgttt tctcttgggt attttcagaa gtcccactat
     7921 tttctaaatg ttctttggga ttttgatcat aattttgaaa ttcgactttt tcctgagaaa
     7981 acagtgcttc attaacgtag tcataaccta agtatattaa gaataacaat aacaataaaa
     8041 aaaacaactt cttcattttc agtctcctcc ttaactatat agttggaagt atgaaatatt
     8101 tctagaatct tccccttaaa cggcacaatc tcgtttgaga ttgtgccgtt ttgccccgta
     8161 aattacagtc aattatttcg ccctactgga tttactatta tcaccaatgt agatgaattt
     8221 tcataagtga tacccatgct gaatttaacg aggctgaact gcctgttgac aatgggggtt
     8281 ctgttgcaaa gttttaaatc tactatcaaa taagg
//
LOCUS       DAZKPO010000242         5879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.121, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000242 DAZKPO010000000
VERSION     DAZKPO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.121"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            398..862
                     /locus_tag="ACG8NZ_003065"
     CDS             398..862
                     /locus_tag="ACG8NZ_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0540625.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            846..1475
                     /locus_tag="ACG8NZ_003066"
     CDS             846..1475
                     /locus_tag="ACG8NZ_003066"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0540626.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1829..3307
                     /gene="msr(C)"
                     /locus_tag="ACG8NZ_003067"
     CDS             1829..3307
                     /gene="msr(C)"
                     /locus_tag="ACG8NZ_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0540627.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3354..3800
                     /locus_tag="ACG8NZ_003068"
     CDS             3354..3800
                     /locus_tag="ACG8NZ_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0540628.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3999..4514)
                     /locus_tag="ACG8NZ_003069"
     CDS             complement(3999..4514)
                     /locus_tag="ACG8NZ_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0540629.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4631..5833
                     /locus_tag="ACG8NZ_003070"
     CDS             4631..5833
                     /locus_tag="ACG8NZ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0540630.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1817 a          980 c         1240 g         1842 t
ORIGIN      
        1 ctatttttct tgcacctttt acttgactaa tcgtaggaaa aaggttgtga caacatcttg
       61 tcataacttt tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt
      121 cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa ttatctactg cagaagagct
      181 atcttctata gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca
      241 ttttttccgt tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt
      301 tttttattac acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc
      361 aatcgttgac gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca
      421 ccttttatat caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat
      481 cggtataagt cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct
      541 acaaagcagt ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa
      601 ggtattggaa gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt
      661 gtatgtagcg ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa tggaccataa
      721 agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac
      781 acttttagat aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa
      841 taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg
      901 tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga
      961 cgactgcacc ttcttctgtc aatatgaaac catggcgctt tgtggtcgct gaaagcgagg
     1021 aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt
     1081 ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctaca
     1141 accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga
     1201 tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt
     1261 ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc
     1321 caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt
     1381 atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc
     1441 gtttagcacc agaaaagatc actatgttcg aataaaaggt tttcagttac tattcagtag
     1501 ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac
     1561 tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat
     1621 gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg
     1681 tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga
     1741 tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg
     1801 ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct
     1861 acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca
     1921 agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat
     1981 agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct
     2041 gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt
     2101 ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat
     2161 cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac
     2221 ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac
     2281 gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga
     2341 agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa
     2401 ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg
     2461 attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag
     2521 taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag
     2581 tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg
     2641 agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga
     2701 actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac
     2761 attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga
     2821 aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct
     2881 tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga
     2941 agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag
     3001 tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag
     3061 tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt
     3121 gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct
     3181 aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg
     3241 tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa
     3301 tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta
     3361 caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga
     3421 tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc
     3481 tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga
     3541 aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta
     3601 tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg
     3661 aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga
     3721 ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag
     3781 caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt
     3841 tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc
     3901 tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc
     3961 tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt
     4021 tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg
     4081 atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt
     4141 tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct
     4201 gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta
     4261 ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag
     4321 tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg
     4381 tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc
     4441 agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag
     4501 gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa
     4561 gtttccacgt tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag
     4621 agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga
     4681 aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg
     4741 agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc
     4801 tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac
     4861 aggtttctta tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat
     4921 cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg
     4981 tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag
     5041 tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg
     5101 aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt
     5161 ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga
     5221 aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca
     5281 taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct ttactttact
     5341 tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt
     5401 tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa
     5461 gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt
     5521 attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt
     5581 tttccaagga attatcaata cactgttgac gacgattgct atggaaatac caggtctgca
     5641 acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc
     5701 atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg
     5761 tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa
     5821 tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DAZKPO010000243         3671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.145, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000243 DAZKPO010000000
VERSION     DAZKPO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.145"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..561
                     /gene="dfrG"
                     /locus_tag="ACG8NZ_003071"
     CDS             64..561
                     /gene="dfrG"
                     /locus_tag="ACG8NZ_003071"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0540631.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(633..2585)
                     /locus_tag="ACG8NZ_003072"
     CDS             complement(633..2585)
                     /locus_tag="ACG8NZ_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0540632.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2592..2828)
                     /locus_tag="ACG8NZ_003073"
     CDS             complement(2592..2828)
                     /locus_tag="ACG8NZ_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540633.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3230..3520
                     /locus_tag="ACG8NZ_003074"
     CDS             3230..3520
                     /locus_tag="ACG8NZ_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0540634.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1142 a          635 c          636 g         1258 t
ORIGIN      
        1 aaaaggtttt ttgatagaaa taagaaatta gatggtaaat tagttaaaaa ggctgtgtta
       61 taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg caaagagaat
      121 gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac aaagggacat
      181 ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc tgacagaaga
      241 aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat tgttcattca
      301 atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt cggaggagaa
      361 cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa aatacatcat
      421 gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa tgaggtattt
      481 gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca tgtatatgaa
      541 agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa agataaagca
      601 caagagcttg ggattactcc aagctctttt tgtcatttga aataaattat atctttccaa
      661 tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt tttatccgct
      721 gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc aaattgagca
      781 tacttcggat tgaagttagc gtaaatatag ctcttcttat caccacgagc tgttgttaat
      841 ggtctttcaa gagatgatat ataacgtctt atctgttgaa taaagctatt ggtcgaatgg
      901 tcattaacat ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga taaatccgtt
      961 ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta atggatgttt gatagggtta
     1021 tcggcatatt tgcgatatct ttctccatct ttactaatag cctcttcgtg gaattgatga
     1081 cttgttagta attcagttag ataatgagat gccaaagtcc gtagaaactt tgttttgaat
     1141 cctctgatat ctccccaatc gagcagttct gcttttgctt gtccgaattc ttttaagcat
     1201 tgttttctgc ttttcgtctt gttaacttgg caaataaaat gatgtgcatc gctcaacctt
     1261 aattctttcg tgaatactct ataaaaggcg tttctaatag agaagtcatc atcactaatc
     1321 attcgccatt cgtcggcatt aaccatttgt ttaattaacc aatagtgagc agttgtagta
     1381 taaggagcat caacatttaa accatcgaca aatctactcc tattagtaat tttccctaat
     1441 tcgtgaagat aagcattcct attttcgcta tcgttagcgg atggttcttg tggataataa
     1501 gaccaatcta acctatcatt tttacgacta aaagtattga gatggtcttc tttgaatttt
     1561 cttgtatctt catttaattc atccattgaa atattccaat cataggcaac atcagaacgg
     1621 aacacatatc ttgataagac ctcagcagat acaactatat acgtttgcat attaaggtct
     1681 tcaaaccccg tatatttctt tgaaccttgt cctttcttac ggacgttatt taggtagtag
     1741 tgcatcttgt ccgtattaag ccacatctcg ttaaatttct ttgtttgaag gggttgtgtc
     1801 tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc actccagctt atgatagtag
     1861 gttccaactc caattcctaa aatatcacaa gtacgactga ctgaaaccct tccaacaacc
     1921 atcttagcga acattggaag aatggtgttc ttttgttggt gataagtagt ggtttcttct
     1981 ctttttggta gaacattagt gaacttctta cacgccttac attgatagcg ttgagatttg
     2041 cccctacttt tccccctttt gtaaaattgc ttcggctcat tgaatggggt tgattcttct
     2101 tccgagcagc cttctttatg aaattgataa tctggctcaa catcttggat gctattgatt
     2161 tctataaggc gtttaatttc ttcaacaaca gaccaattag ataaaggggt tactgtacag
     2221 ttttgcgata cacctctgcc aataggattg tcattgcagt acataccttt atgaccttta
     2281 tctttggaac taccagtcat tttatatcga cttggtttcc cttttacatc tttgaatttg
     2341 tgctgttctt tcccaaaatt acaacaaaac ggattgatgc agtaattata ttgaatggga
     2401 tattcagaac cgttccattt aaaagacgtt ggcaagaaca acattttgtt ataccttgtt
     2461 gcaaatcgtt ttggagttaa taaatcatat tgtttagccc tatcttttaa ttcttcgttt
     2521 gaaatcggaa ttttaacatc tacaatttca tcttccttcg tggctaatct tttaagcttg
     2581 gtcattgtct atcaacccac tctatctttg atacccattt tttcaacaat ccgcttatta
     2641 cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt caataagaat
     2701 tcatccactg cttcgctttc agtacgctct gcatattcag cgtattgctt aactatcacc
     2761 cttacttgct ctgatatttc ccaatccaca gacttagcgt tcttattttt tggctcaaga
     2821 aattgcatag gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt taacacacca
     2881 atttgcattc ctacaataat tatttcacgt tcgcattttt atatacattt taacttacat
     2941 tttgtggtgt aaattaacaa caaacgttga tttaacaata aaaaacgagt atcaaattca
     3001 gttgcaattt gatactcatt taggtttaca ttttcacttt ttgtactcca aaactgaact
     3061 acttaaatct atactgctgc ctcttttttt tagtacatgt ccttcatctt cgcaggaagt
     3121 gccaatatga ttttaagaaa ggtaatagac aaaaagtcaa aaagataaaa cagagtttct
     3181 tctttacaaa gggaatgtat gttcgtataa tggtattgag gtgaaggtta tggagaaaaa
     3241 atatggaaca gtagataaga tccgtacttt gcaagttagt gccagaccgt tgatccgttt
     3301 cagtttaaat ggggtcaatt gtttgattgc ttcccatagt ttgaacttcc tagctgaagt
     3361 agacgaaggg atgaagttag tcgttacagg ttattataat aatagaaagc aatttgttgt
     3421 gagatcctat catgtgttag ggaaacccaa aattgtggta gaatatgaga aaagtctata
     3481 cccaagaaaa aaggtccgac aagatgaaag aataaactag aaaacaagcg gctgagacaa
     3541 aaatgtccta gccgcttgtt ttggaaagat ggatgcagac gatgggattt gaacccacac
     3601 gagcatacgc tcacaagaac ctcaatctat atataactac atgtatttaa gtatttgtga
     3661 tgaatcacta a
//
LOCUS       DAZKPO010000244         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000244 DAZKPO010000000
VERSION     DAZKPO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.149"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG8NZ_003075"
     CDS             3..188
                     /locus_tag="ACG8NZ_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0540635.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG8NZ_003076"
     CDS             203..1072
                     /locus_tag="ACG8NZ_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0540636.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG8NZ_003077"
     CDS             1053..1787
                     /locus_tag="ACG8NZ_003077"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0540637.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG8NZ_003078"
     CDS             1820..2728
                     /locus_tag="ACG8NZ_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0540638.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG8NZ_003079"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG8NZ_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKPO010000245         2877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.159, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000245 DAZKPO010000000
VERSION     DAZKPO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.159"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1175
                     /locus_tag="ACG8NZ_003080"
     CDS             207..1175
                     /locus_tag="ACG8NZ_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0540639.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1168..2199
                     /gene="vanA"
                     /locus_tag="ACG8NZ_003081"
     CDS             1168..2199
                     /gene="vanA"
                     /locus_tag="ACG8NZ_003081"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0540640.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2205..2813
                     /locus_tag="ACG8NZ_003082"
     CDS             2205..2813
                     /locus_tag="ACG8NZ_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0540641.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          852 a          552 c          698 g          775 t
ORIGIN      
        1 tataattttt taggaaaatc tcaaggttat ctttactttt tcttaggaaa ttaacaattt
       61 aatattaaga aacggctcgt tcttacacgg tagacttaat accgtaagaa cgagccgttt
      121 tcgttcttca gagaaagatt tgacaagatt accattggca tccccgtttt atttggtgcc
      181 tttcacagaa agggttggtc ttaattatga ataacatcgg cattactgtt tatggatgtg
      241 agcaggatga ggcagatgca ttccatgctc tttcgcctcg ctttggcgtt atggcaacga
      301 taattaacgc caacgtgtcg gaatccaacg ccaaatccgc gcctttcaat caatgtatca
      361 gtgtgggaca taaatcagag atttccgcct ctattcttct tgcgctgaag agagccggtg
      421 tgaaatatat ttctacccga agcatcggct gcaatcatat agatacaact gctgctaaga
      481 gaatgggcat cactgtcgac aatgtggcgt actcgccgga tagcgttgcc gattatacta
      541 tgatgctaat tcttatggca gtacgcaacg taaaatcgat tgtgcgctct gtggaaaaac
      601 atgatttcag gttggacagc gaccgtggca aggtactcag cgacatgaca gttggtgtgg
      661 tgggaacggg ccagataggc aaagcggtta ttgagcggct gcgaggattt ggatgtaaag
      721 tgttggctta tagtcgcagc cgaagtatag aggtaaacta tgtaccgttt gatgagttgc
      781 tgcaaaatag cgatatcgtt acgcttcatg tgccgctcaa tacggatacg cactatatta
      841 tcagccacga acaaatacag agaatgaagc aaggagcatt tcttatcaat actgggcgcg
      901 gtccacttgt agatacctat gagttggtta aagcattaga aaacgggaaa ctgggcggtg
      961 ccgcattgga tgtattggaa ggagaggaag agtttttcta ctctgattgc acccaaaaac
     1021 caattgataa tcaattttta cttaaacttc aaagaatgcc taacgtgata atcacaccgc
     1081 atacggccta ttataccgag caagcgttgc gtgataccgt tgaaaaaacc attaaaaact
     1141 gtttggattt tgaaaggaga caggagcatg aatagaataa aagttgcaat actgtttggg
     1201 ggttgctcag aggagcatga cgtatcggta aaatctgcaa tagagatagc cgctaacatt
     1261 aataaagaaa aatacgagcc gttatacatt ggaattacga aatctggtgt atggaaaatg
     1321 tgcgaaaaac cttgcgcgga atgggaaaac gacaattgct attcagctgt actctcgccg
     1381 gataaaaaaa tgcacggatt acttgttaaa aagaaccatg aatatgaaat caaccatgtt
     1441 gatgtagcat tttcagcttt gcatggcaag tcaggtgaag atggatccat acaaggtctg
     1501 tttgaattgt ccggtatccc ttttgtaggc tgcgatattc aaagctcagc aatttgtatg
     1561 gacaaatcgt tgacatacat cgttgcgaaa aatgctggga tagctactcc cgccttttgg
     1621 gttattaata aagatgatag gccggtggca gctacgttta cctatcctgt ttttgttaag
     1681 ccggcgcgtt caggctcatc cttcggtgtg aaaaaagtca atagcgcgga cgaattggac
     1741 tacgcaattg aatcggcaag acaatatgac agcaaaatct taattgagca ggctgtttcg
     1801 ggctgtgagg tcggttgtgc ggtattggga aacagtgccg cgttagttgt tggcgaggtg
     1861 gaccaaatca ggctgcagta cggaatcttt cgtattcatc aggaagtcga gccggaaaaa
     1921 ggctctgaaa acgcagttat aaccgttccc gcagaccttt cagcagagga gcgaggacgg
     1981 atacaggaaa cggcaaaaaa aatatataaa gcgctcggct gtagaggtct agcccgtgtg
     2041 gatatgtttt tacaagataa cggccgcatt gtactgaacg aagtcaatac tctgcccggt
     2101 ttcacgtcat acagtcgtta tccccgtatg atggccgctg caggtattgc acttcccgaa
     2161 ctgattgacc gcttgatcgt attagcgtta aaggggtgat aagcatggaa ataggattta
     2221 cttttttaga tgaaatagta cacggtgttc gttgggacgc taaatatgcc acttgggata
     2281 atttcaccgg aaaaccggtt gacggttatg aagtaaatcg cattgtaggg acatacgagt
     2341 tggctgaatc gcttttgaag gcaaaagaac tggctgctac ccaagggtac ggattgcttc
     2401 tatgggacgg ttaccgtcct aagcgtgctg taaactgttt tatgcaatgg gctgcacagc
     2461 cggaaaataa cctgacaaag gaaagttatt atcccaatat tgaccgaact gagatgattt
     2521 caaaaggata cgtggcttca aaatcaagcc atagccgcgg cagtgccatt gatcttacgc
     2581 tttatcgatt agacacgggt gagcttgtac caatggggag ccgatttgat tttatggatg
     2641 aacgctctca tcatgcggca aatggaatat catgcaatga agcgcaaaat cgcagacgtt
     2701 tgcgctccat catggaaaac agtgggtttg aagcatatag cctcgaatgg tggcactatg
     2761 tattaagaga cgaaccatac cccaatagct attttgattt ccccgttaaa taaactttta
     2821 accgttgcac ggacaaacgg ttctgttgca aagttttaaa taaagaataa aatccct
//
LOCUS       DAZKPO010000246         1924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.179, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000246 DAZKPO010000000
VERSION     DAZKPO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.179"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8NZ_003083"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8NZ_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0540642.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8NZ_003084"
     CDS             763..1917
                     /locus_tag="ACG8NZ_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0540643.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          628 a          369 c          421 g          506 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgt
//
LOCUS       DAZKPO010000247         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.196, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000247 DAZKPO010000000
VERSION     DAZKPO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.196"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8NZ_003085"
     CDS             26..76
                     /locus_tag="ACG8NZ_003085"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0540644.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8NZ_003086"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8NZ_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0540645.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DAZKPO010000248          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.207, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000248 DAZKPO010000000
VERSION     DAZKPO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.207"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACG8NZ_003087"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACG8NZ_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACG8NZ_003088"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACG8NZ_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          325 a          171 c          121 g          326 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt ata
//
LOCUS       DAZKPO010000249          824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3334
            SAMEA115706648-rid22575633.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAZKPO010000249 DAZKPO010000000
VERSION     DAZKPO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706648
            Sequence Read Archive: ERR13196383
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287717.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287717.1
            Genome Coverage       :: 182x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:59:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 3,029
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 52 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706648-rid22575633.guided.211"
                     /isolate="VRE3334"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..656
                     /gene="aac(6')"
                     /locus_tag="ACG8NZ_003089"
     CDS             108..656
                     /gene="aac(6')"
                     /locus_tag="ACG8NZ_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0540646.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          295 a          126 c          186 g          217 t
ORIGIN      
        1 ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt
       61 tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg
      121 aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg agactgactt
      181 ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac
      241 gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc
      301 aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga
      361 accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa
      421 tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc
      481 tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg
      541 aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg
      601 acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa
      661 aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact
      721 tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt
      781 accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaa
//
